LOCUS IBMc398 7738 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-187)-ANG-M86N-cat-Plux2-B3 2-M86C-S-mCherry*(188-236)-H6-T ACCESSION IBMc398 VERSION IBMc398.1 FEATURES Location/Qualifiers RBS 5586..5598 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS 3305..3604 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" promoter 5524..5577 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Plux2" CDS 2738..3295 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator 5475..5515 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0012" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind complement(6117..6136) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" RBS complement(5417..5431) /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" rep_origin 6583..7407 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(4655..5410) /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="luxR" misc_feature 6002..6022 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator complement(4565..4621) /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" terminator 5949..6001 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" promoter complement(4459..4556) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 5920..5937 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS complement(3799..4458) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 5773..5919 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator complement(3714..3770) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5608..5769 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" promoter complement(5440..5474) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="BBa_J23117" terminator 3616..3705 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 TGGAtgcatc tcgggagata gtttgatcag cttggcgagc acagggaaaa gagttcctat 3361 taaggatttg ttaggcgaaa aagattttga aatatgggca attaatgaac agacgatgaa 3421 gctggaatca gctaaagtta gtcgtgtatt ttgtaccggc aaaaagctag tctatactct 3481 aaaaactcga ctaggtagaa ctatcaaggc aacagcaaat catagatttt taactattga 3541 tggttggaaa agattagatg agctatcttt aaaagagcat attgctctac cccgtaaact 3601 agaaTAATAC TAGAGttgag aagagaaaag aaaaccgccg atcctgtcca ccgcattact 3661 gcaaggtagt ggacaagacc ggcggtctta agttttttgg ctgaaTACTA GAGcctttgg 3721 tcgAAAAAAA AAGCCCGCAC TGTCAGGTGC GGGCTTTTTT ctgtgtttcc TACTAGAGCA 3781 AGCGAGCTCG ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC 3841 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG 3901 CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA 3961 GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC 4021 TGAAACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA 4081 ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT 4141 CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT 4201 ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT 4261 CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT 4321 CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA 4381 CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC 4441 AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA 4501 TACGCCCGGT AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCTACT 4561 AGAGGGACCA AAACGAAAAA AGACGCTTTT CAGCGTCTTA TTGTTCGTCT TTGGTACCGA 4621 GCTCTAGTAG TGATCTACAC TAGCACTATC AGTGTTATTA ATTTTTAAAG TATGGGCAAT 4681 CAATTGCTCC TGTTAAAATT GCTTTAGAAA TACTTTGGCA GCGGTTTGTT GTATTGAGTT 4741 TCATTTGCGC ATTGGTTAAA TGGAAAGTGA CAGTACGCTC ACTGCAACCT AATATTTTTG 4801 AAATATCCCA AGAGCTTTTT CCTTCGCATG CCCACGCTAA ACATTCTTTT TCTCTTTTGG 4861 TTAAATCGTT GTTTGATTTA TTATTTGCTA TATTTATTTT TCGATAATTA TCAACTAGAG 4921 AAGGAACAAT TAATGGTATG TTCATACACG CATGTAAAAA TAAACTATCT ATATAGTTGT 4981 CTTTTTCTGA ATGTGCAAAA CTAAGCATTC CGAAGCCATT GTTAGCCGTA TGAATAGGGA 5041 AACTAAACCC AGTGATAAGA CCTGATGTTT TCGCTTCTTT AATTACATTT GGAGATTTTT 5101 TATTTACAGC ATTGTTTTCA AATATATTCC AATTAATTGG TGAATGATTG GAGTTAGAAT 5161 AATCTACTAT AGGATCATAT TTTATTAAAT TAGCGTCATC ATAATATTGC CTCCATTTTT 5221 TAGGGTAATT ATCTAGGATT GAAATATCAG ATTTAACCAT AGAATGAGGA TAAATGATCG 5281 CGAGTAAATA ATATTCACAA TGTACCATTT TAGTCATATC AGATAAGCAT TGATTAATAT 5341 CATTATTGCT TCTACAAGCT TTAATTTTAT TAATTATTCT GTATGTGTCG TCGGCATTTA 5401 TGTTTTTCAT CTAGTATTTC TCCTCTTTAA TCTCTAGTAG CTAGCACAAT CCCTAGGACT 5461 GAGCTAGCTG TCAATCACAC TGGCTCACCT TCGGGTGGGC CTTTCTGCGT TTATATACTA 5521 GACACCTGTA GGATCGTACA GGTTTACGCA AGAAAATGGT TTGTTACTTT CGAATAAtac 5581 tagagtcaca caggaaagta ctagATGagc tcctctttac aattggcacc agaaatagaa 5641 aagttgcctc agagtgatat ttactgggac cccatcgttt ctattacgga gactggagtc 5701 gaagaggttt ttgatttgac tgtgccagga ctacgtaact ttgtcgccaa tgacatcatt 5761 gtacataacT CAgccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 5821 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 5881 ggccgccact ccaccggcgg catggacgag ctgtacaagc atcatcacca tcaccactaa 5941 tactagagaa cgcatgagAA AGCCCCCGGA AGATCACCTT CCGGGGGCTT Ttttattgcg 6001 ctactagtag cggccgctgc aggagtcact aagggttagt tagttagatt agcagaaagt 6061 caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc attggggctc 6121 actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgct 6181 agagatggaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6241 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg atctcgcggt 6301 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6361 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6421 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6481 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6541 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct 6601 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 6661 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 6721 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 6781 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 6901 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 6961 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 7021 gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt 7081 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 7141 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 7201 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 7261 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc 7321 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa 7381 catagtaagc cagtatacac tccgctagcg ctgaggtctg cctcgtgaag aaggtgttgc 7441 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt 7501 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga 7561 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt 7621 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 7681 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgtt //