LOCUS IBMc404 5709 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-52)-NGG-M86N-T ACCESSION IBMc404 VERSION IBMc404.1 FEATURES Location/Qualifiers primer_bind 5607..5624 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" primer_bind complement(2250..2266) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" CDS 1462..1761 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" terminator complement(1871..1927) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(3418..4566) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" CDS complement(1956..2096) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" terminator 2196..2290 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator 1773..1862 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter 2343..2440 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 1297..1452 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 2441..3100 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTGG Atgcatctcg ggagatagtt tgatcagctt ggcgagcaca 1501 gggaaaagag ttcctattaa ggatttgtta ggcgaaaaag attttgaaat atgggcaatt 1561 aatgaacaga cgatgaagct ggaatcagct aaagttagtc gtgtattttg taccggcaaa 1621 aagctagtct atactctaaa aactcgacta ggtagaacta tcaaggcaac agcaaatcat 1681 agatttttaa ctattgatgg ttggaaaaga ttagatgagc tatctttaaa agagcatatt 1741 gctctacccc gtaaactaga aTAATACTAG AGttgagaag agaaaagaaa accgccgatc 1801 ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt tttttggctg 1861 aaTACTAGAG cctttggtcg AAAAAAAAAG CCCGCACTGT CAGGTGCGGG CTTTTTTctg 1921 tgtttccTAC TAGAGCAAGC GAGCTCGATA TCAAATTACG CCCCGCCCTG CCACTCATCG 1981 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA 2041 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG 2101 GGATCGATCG ATCGATCgga tcctctagag tcgacctgca ggcatgcaag cttgcggccg 2161 cgtcgtgact gggaaaaccc tggcgactag tcttggactc ctgttgatag atccagtaat 2221 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat 2281 tggtgagaat ccaggggtcc ccaataatta cgatttaaat tggcgaaaat gagacgttga 2341 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt 2401 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg 2461 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc 2521 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac 2581 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat 2641 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag 2701 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag 2761 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta 2821 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc 2881 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt 2941 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct 3001 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga 3061 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgactttt gtccttttcc 3121 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc 3181 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg 3241 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact 3301 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag 3361 gccggccgcg atgcaggtgg ctgctgaacc cccagccgga actgacccca caaggcccta 3421 gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg 3481 caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga 3541 tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa 3601 atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt 3661 cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg 3721 ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag 3781 gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc 3841 ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta 3901 gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg 3961 ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc 4021 cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg 4081 cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca 4141 cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag 4201 caacgcggcc tgcttggctt cgctgacctg ttttgccagg tcctcgccgg cggtttttcg 4261 cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc 4321 ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg 4381 agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacta tcgagccgac 4441 ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc 4501 ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg 4561 attcattcac cctccttgcg ggattgcccc ggaattaatt ccccggatcg atccgtcgat 4621 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag 4681 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat 4741 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt 4801 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac 4861 cgtttctgcg gactggcttt ctacgtggct gccatttttg gggtgaggcc gttcgcggcc 4921 gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga 4981 agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa 5041 aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa 5101 aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt 5161 caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc 5221 tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct 5281 tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc 5341 tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc 5401 gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt 5461 ttccgcgagg tatccacaac gccggcggcc ctacatggct ctgctgtagt gagtgggttg 5521 cgctccggca gcggtcctga tcccccgcag aaaaaaagga tctcaagaag atcctttgat 5581 cttttctacg gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt 5641 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat 5701 ttgatgcct //