LOCUS IBMc409 5886 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-111)-LDG-M86N-T ACCESSION IBMc409 VERSION IBMc409.1 FEATURES Location/Qualifiers promoter 2520..2617 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 1297..1629 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" CDS 2618..3277 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind 5784..5801 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind complement(2427..2443) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" terminator complement(2048..2104) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(3595..4743) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" CDS 1639..1938 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator 2373..2467 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" terminator 1950..2039 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS complement(2133..2273) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTATTGAAC GTAGCTGGGA AGCAGTTGTT 1561 GTTGCAATGC TGGATGCAGT TCATAGTCCG CAGAGCAAAC AGTTTAGCGA AATTCTGATT 1621 TATCAGGGTC TGGATGGAtg catctcggga gatagtttga tcagcttggc gagcacaggg 1681 aaaagagttc ctattaagga tttgttaggc gaaaaagatt ttgaaatatg ggcaattaat 1741 gaacagacga tgaagctgga atcagctaaa gttagtcgtg tattttgtac cggcaaaaag 1801 ctagtctata ctctaaaaac tcgactaggt agaactatca aggcaacagc aaatcataga 1861 tttttaacta ttgatggttg gaaaagatta gatgagctat ctttaaaaga gcatattgct 1921 ctaccccgta aactagaaTA ATACTAGAGt tgagaagaga aaagaaaacc gccgatcctg 1981 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaT 2041 ACTAGAGcct ttggtcgAAA AAAAAAGCCC GCACTGTCAG GTGCGGGCTT TTTTctgtgt 2101 ttccTACTAG AGCAAGCGAG CTCGATATCA AATTACGCCC CGCCCTGCCA CTCATCGCAG 2161 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 2221 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGGA 2281 TCGATCGATC GATCggatcc tctagagtcg acctgcaggc atgcaagctt gcggccgcgt 2341 cgtgactggg aaaaccctgg cgactagtct tggactcctg ttgatagatc cagtaatgac 2401 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 2461 tgagaatcca ggggtcccca ataattacga tttaaattgg cgaaaatgag acgttgatcg 2521 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt 2581 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata 2641 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt 2701 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt 2761 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa 2821 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt 2881 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga 2941 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg 3001 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa 3061 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc 3121 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc 3181 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt 3241 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gacttttgtc cttttccgct 3301 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca 3361 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt 3421 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc 3481 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc 3541 ggccgcgatg caggtggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg 3601 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa 3661 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc 3721 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata 3781 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt 3841 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga 3901 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg 3961 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc 4021 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa 4081 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca 4141 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt 4201 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt 4261 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg 4321 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa 4381 cgcggcctgc ttggcttcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt 4441 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc 4501 ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc 4561 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggactatcg agccgacgga 4621 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc 4681 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt 4741 cattcaccct ccttgcggga ttgccccgga attaattccc cggatcgatc cgtcgatctt 4801 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc 4861 ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa 4921 accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg 4981 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt 5041 ttctgcggac tggctttcta cgtggctgcc atttttgggg tgaggccgtt cgcggccgag 5101 gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg 5161 gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac 5221 aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa 5281 cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa 5341 taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca 5401 tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt 5461 ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg 5521 ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg 5581 tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc 5641 cgcgaggtat ccacaacgcc ggcggcccta catggctctg ctgtagtgag tgggttgcgc 5701 tccggcagcg gtcctgatcc cccgcagaaa aaaaggatct caagaagatc ctttgatctt 5761 ttctacggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac 5821 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg 5881 atgcct //