LOCUS IBMc420 5764 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(78-213)-Ter ACCESSION IBMc420 VERSION IBMc420.1 FEATURES Location/Qualifiers misc_feature 3286..3360 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" promoter complement(2036..2133) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" terminator 2893..3147 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" primer_bind complement(4143..4162) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 2281..2883 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" CDS 3391..3552 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" CDS complement(1517..2035) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" promoter 3220..3285 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" RBS 2253..2280 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 3975..4027 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter 2199..2252 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" misc_feature 1494..1494 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" misc_feature 4028..4048 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator 3148..3200 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" rep_origin 4609..5433 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator 2134..2194 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 3556..3963 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" RBS 3369..3381 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT 1561 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA AACGAAAAAC ATATTCTCAA 1621 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 1681 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG 1741 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT 1801 CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA 1861 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA 1921 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT 1981 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG 2041 CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT 2101 CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCctcggta ccaaattcca gaaaagaggc 2161 ctcccgaaag gggggccttt tttcgttttg gtccaatggc ggcgcgccat cgaatggtgc 2221 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcat gggggtgaat 2281 atggcacgta ccccgagccg tagcagcatt ggtagcctgc gtagtccgca tacccataaa 2341 gcaattctga ccagcaccat tgaaatcctg aaagaatgtg gttatagcgg tctgagcatt 2401 gaaagcgtgg cacgtcgcgc cggtgcaggc aaaccgacca tttatcgttg gtggaccaac 2461 aaagcagcac tgattgccga agtgtatgaa aatgaaatcg aacaggtacg taaatttccg 2521 gatttgggta gctttaaagc cgatctggat tttctgctgc ataatctgtg gaaagtttgg 2581 cgtgaaacca tttgtggtga agcatttcgt tgtgttattg cagaagcaca gttggaccct 2641 gtaaccctga cccaactgaa agatcagttt atggaacgtc gtcgtgagat accgaaaaaa 2701 ctggttgaag atgccattag caatggtgaa ctgccgaaag atatcaatcg tgaactgctg 2761 ctggatatga tttttggttt ttgttggtat cgcctgctga ccgaacagtt gaccgttgaa 2821 caggatattg aagaatttac cttcctgctg attaatggtg tttgtccggg tacacagtgt 2881 tgataaggat cctaattggt aacgaatcag acaattgacg gctcgaggga gtagcatagg 2941 gtttgcagaa tccctgcttc gtccatttga caggcacatt atgcatcgat gataagctgt 3001 caaacatgag cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg 3061 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt 3121 cgggctcatg agcaaatatt ttatctgcca attattgaag gcctccctaa cggggggcct 3181 ttttttgttt ctggtctccc gcttaacgat cgttggctgc gacgtacggt ggaatctgat 3241 tcgttaccaa ttgacatgat acgaaacgta ccgtatcgtt aaggtagctg tcaccggatg 3301 tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa 3361 tactagagtc acacaggaaa gtactagATG agctcctctt tacaattggc accagaaata 3421 gaaaagttgc ctcagagtga tatttactgg gaccccatcg tttctattac ggagactgga 3481 gtcgaagagg tttttgattt gactgtgcca ggactacgta actttgtcgc caatgacatc 3541 attgtacata acTCACTGGG TATTGAACGT AGCTGGGAAG CAGTTGTTGT TGCAATGCTG 3601 GATGCAGTTC ATAGTCCGCA GAGCAAACAG TTTAGCGAAA TTCTGATTTA TCAGGGTCTG 3661 GATGAAAGCG GTCTGATTCA TAATCGTATG GTTCAGGCAA GCGATCGTTT TCTGCAATAT 3721 ATTCATCAGG TTCTGCGTCA TGCAGTTACC CAGGGTGAAC TGCCGATTAA TCTGGATCTG 3781 CAAACCAGCA TTGGTGTTTT TAAAGGTCTG ATTACCGGTC TGCTGTATGA AGGTCTGCGT 3841 AGCAAAGATC AGCAGGCACA GATTATCAAA GTTGCACTGG GTAGCTTTTG GGCACTGCTG 3901 CGTGAACCGC CTCGTTTTCT GCTGTGTGAA GAAGCACAGA TTAAACAGGT GAAATCCTTC 3961 GAATAAtact agagaacgca tgagAAAGCC CCCGGAAGAT CACCTTCCGG GGGCTTTttt 4021 attgcgctac tagtagcggc cgctgcagga gtcactaagg gttagttagt tagattagca 4081 gaaagtcaaa agcctccgac cggaggcttt tgactaaaac ttcccttggg gttatcattg 4141 gggctcactc aaaggcggta atcagataaa aaaaatcctt agctttcgct aaggatgatt 4201 tctgctagag atggaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 4261 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggatct 4321 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 4381 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 4441 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 4501 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 4561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagacccctt aataagatga 4621 tcttcttgag atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc 4681 ttgcagggcg gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg 4741 cttggaggag cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac 4801 ttcaagacta actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg 4861 tctttccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac 4921 ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag 4981 gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag 5041 gaacaggaga gcgcacgagg gagccgccag gggaaacgcc tggtatcttt atagtcctgt 5101 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag 5161 cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca 5221 tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc 5281 gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga 5341 cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag 5401 tgccaacata gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg 5461 tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc 5521 acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc 5581 cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt 5641 tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga 5701 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg 5761 tgtt //