LOCUS IBMc421 5668 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(110-213)-Ter ACCESSION IBMc421 VERSION IBMc421.1 FEATURES Location/Qualifiers CDS 3556..3867 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" promoter complement(2036..2133) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" misc_feature 3286..3360 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" terminator 2893..3147 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" primer_bind complement(4047..4066) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" RBS 2253..2280 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" CDS complement(1517..2035) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 3391..3552 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 3148..3200 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" promoter 3220..3285 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" promoter 2199..2252 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" misc_feature 1494..1494 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 3879..3931 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" RBS 3369..3381 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" misc_feature 3932..3952 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator 2134..2194 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" rep_origin 4513..5337 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 2281..2883 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT 1561 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA AACGAAAAAC ATATTCTCAA 1621 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 1681 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG 1741 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT 1801 CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA 1861 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA 1921 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT 1981 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG 2041 CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT 2101 CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCctcggta ccaaattcca gaaaagaggc 2161 ctcccgaaag gggggccttt tttcgttttg gtccaatggc ggcgcgccat cgaatggtgc 2221 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcat gggggtgaat 2281 atggcacgta ccccgagccg tagcagcatt ggtagcctgc gtagtccgca tacccataaa 2341 gcaattctga ccagcaccat tgaaatcctg aaagaatgtg gttatagcgg tctgagcatt 2401 gaaagcgtgg cacgtcgcgc cggtgcaggc aaaccgacca tttatcgttg gtggaccaac 2461 aaagcagcac tgattgccga agtgtatgaa aatgaaatcg aacaggtacg taaatttccg 2521 gatttgggta gctttaaagc cgatctggat tttctgctgc ataatctgtg gaaagtttgg 2581 cgtgaaacca tttgtggtga agcatttcgt tgtgttattg cagaagcaca gttggaccct 2641 gtaaccctga cccaactgaa agatcagttt atggaacgtc gtcgtgagat accgaaaaaa 2701 ctggttgaag atgccattag caatggtgaa ctgccgaaag atatcaatcg tgaactgctg 2761 ctggatatga tttttggttt ttgttggtat cgcctgctga ccgaacagtt gaccgttgaa 2821 caggatattg aagaatttac cttcctgctg attaatggtg tttgtccggg tacacagtgt 2881 tgataaggat cctaattggt aacgaatcag acaattgacg gctcgaggga gtagcatagg 2941 gtttgcagaa tccctgcttc gtccatttga caggcacatt atgcatcgat gataagctgt 3001 caaacatgag cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg 3061 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt 3121 cgggctcatg agcaaatatt ttatctgcca attattgaag gcctccctaa cggggggcct 3181 ttttttgttt ctggtctccc gcttaacgat cgttggctgc gacgtacggt ggaatctgat 3241 tcgttaccaa ttgacatgat acgaaacgta ccgtatcgtt aaggtagctg tcaccggatg 3301 tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa 3361 tactagagtc acacaggaaa gtactagATG agctcctctt tacaattggc accagaaata 3421 gaaaagttgc ctcagagtga tatttactgg gaccccatcg tttctattac ggagactgga 3481 gtcgaagagg tttttgattt gactgtgcca ggactacgta actttgtcgc caatgacatc 3541 attgtacata acTCACAGGG TCTGGATGAA AGCGGTCTGA TTCATAATCG TATGGTTCAG 3601 GCAAGCGATC GTTTTCTGCA ATATATTCAT CAGGTTCTGC GTCATGCAGT TACCCAGGGT 3661 GAACTGCCGA TTAATCTGGA TCTGCAAACC AGCATTGGTG TTTTTAAAGG TCTGATTACC 3721 GGTCTGCTGT ATGAAGGTCT GCGTAGCAAA GATCAGCAGG CACAGATTAT CAAAGTTGCA 3781 CTGGGTAGCT TTTGGGCACT GCTGCGTGAA CCGCCTCGTT TTCTGCTGTG TGAAGAAGCA 3841 CAGATTAAAC AGGTGAAATC CTTCGAATAA tactagagaa cgcatgagAA AGCCCCCGGA 3901 AGATCACCTT CCGGGGGCTT Ttttattgcg ctactagtag cggccgctgc aggagtcact 3961 aagggttagt tagttagatt agcagaaagt caaaagcctc cgaccggagg cttttgacta 4021 aaacttccct tggggttatc attggggctc actcaaaggc ggtaatcaga taaaaaaaat 4081 ccttagcttt cgctaaggat gatttctgct agagatggaa tagactggat ggaggcggat 4141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 4201 tctggagccg gtgagcgtgg atctcgcggt atcattgcag cactggggcc agatggtaag 4261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 4321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 4381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 4441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 4501 gcgtcagacc ccttaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct 4561 tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc 4621 aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca 4681 gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc 4741 tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga 4801 taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac 4861 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa 4921 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccaggggaaa 4981 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc 5041 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact 5101 tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt 5161 tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat 5221 atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct gccacatgaa 5281 gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac tccgctagcg 5341 ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc 5401 atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt 5461 ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat 5521 ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa 5581 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 5641 ttacataaac agtaatacaa ggggtgtt //