##gff-version 3
##sequence-region IBMc424 9 5316
IBMc424	.	biological_region	3911	3931	.	+	0	ID=annotation3;Name=RFC10 Suffix
IBMc424	.	CDS	1517	2035	.	-	0	ID=annotation1;Name=cat (BsmBI-)
IBMc424	.	terminator	3858	3910	.	+	0	ID=annotation0;Name=ECK120033736
IBMc424	.	CDS	3391	3552	.	+	0	ID=annotation15;Name=M86C
IBMc424	.	CDS	2281	2883	.	+	0	ID=annotation4;Name=PhlF
IBMc424	.	promoter	9	97	.	+	0	ID=annotation18;Name=tetR promoter
IBMc424	.	CDS	3556	3846	.	+	0	ID=annotation8;Name=SrpR
IBMc424	.	terminator	3148	3200	.	+	0	ID=annotation14;Name=L3S3P21
IBMc424	.	primer_binding_site	1371	1390	.	+	0	ID=annotation12;Name=VF2
IBMc424	.	promoter	2199	2252	.	+	0	ID=annotation6;Name=PlacIQ
IBMc424	.	CDS	98	1291	.	+	0	ID=annotation2;Name=tetR
IBMc424	.	biological_region	1494	1494	.	+	0	ID=annotation7;Name=RFC10 Prefix (full)
IBMc424	.	promoter	2036	2133	.	-	0	ID=annotation17;Name=cat promoter
IBMc424	.	terminator	2893	3147	.	+	0	ID=annotation16;Name=IOT
IBMc424	.	ribosome_entry_site	2253	2280	.	+	0	ID=annotation9;Name=phl2
IBMc424	.	primer_binding_site	4026	4045	.	-	0	ID=annotation13;Name=VR
IBMc424	.	insulator	3286	3360	.	+	0	ID=annotation10;Name=RiboJ
IBMc424	.	terminator	2134	2194	.	+	0	ID=annotation11;Name=L3S2P21
IBMc424	.	origin_of_replication	4492	5316	.	+	0	ID=annotation5;Name=p15A origin of replication
IBMc424	.	ribosome_entry_site	3369	3381	.	+	0	ID=annotation20;Name=BBa_B0032
IBMc424	.	promoter	3220	3285	.	+	0	ID=annotation19;Name=PPhlF
>IBMc424
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAA
CTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAAC
ACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTC
AGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGA
AATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGG
TCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG
TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCT
GAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCC
ctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcg
aatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtac
cccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctg
aaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgtt
ggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttggg
tagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcattt
cgttgtgttattgcagaagcacagttggaccctgtaaccctgacccaactgaaagatcagtttatggaacgtcgtcgtg
agataccgaaaaaactggttgaagatgccattagcaatggtgaactgccgaaagatatcaatcgtgaactgctgctgga
tatgatttttggtttttgttggtatcgcctgctgaccgaacagttgaccgttgaacaggatattgaagaatttaccttc
ctgctgattaatggtgtttgtccgggtacacagtgttgataaggatcctaattggtaacgaatcagacaattgacggct
cgagggagtagcatagggtttgcagaatccctgcttcgtccatttgacaggcacattatgcatcgatgataagctgtca
aacatgagcagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatat
cgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcaaatattttatctgccaattattgaag
gcctccctaacggggggcctttttttgtttctggtctcccgcttaacgatcgttggctgcgacgtacggtggaatctga
ttcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggtagctgtcaccggatgtgctttccggtctgatga
gtccgtgaggacgaaacagcctctacaaataattttgtttaatactagagtcacacaggaaagtactagATGagctcct
ctttacaattggcaccagaaatagaaaagttgcctcagagtgatatttactgggaccccatcgtttctattacggagac
tggagtcgaagaggtttttgatttgactgtgccaggactacgtaactttgtcgccaatgacatcattgtacataacTCA
CTGATTCATAATCGTATGGTTCAGGCAAGCGATCGTTTTCTGCAATATATTCATCAGGTTCTGCGTCATGCAGTTACCC
AGGGTGAACTGCCGATTAATCTGGATCTGCAAACCAGCATTGGTGTTTTTAAAGGTCTGATTACCGGTCTGCTGTATGA
AGGTCTGCGTAGCAAAGATCAGCAGGCACAGATTATCAAAGTTGCACTGGGTAGCTTTTGGGCACTGCTGCGTGAACCG
CCTCGTTTTCTGCTGTGTGAAGAAGCACAGATTAAACAGGTGAAATCCTTCGAATAAtactagagaacgcatgagAAAG
CCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcactaagggttagtt
agttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctc
actcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactgga
tggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagc
cggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacg
acggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaac
tgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagat
cctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatga
tcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcga
aggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagt
ttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgc
atgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcata
cagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcgga
atgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatag
tcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaac
ggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagc
catttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacata
ttctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacata
gtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcg
ccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaa
cttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgattt
attcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataa
aactgtctgcttacataaacagtaatacaaggggtgtt