LOCUS IBMc426 5485 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(171-213)-Ter ACCESSION IBMc426 VERSION IBMc426.1 FEATURES Location/Qualifiers CDS 3391..3552 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" promoter 3220..3285 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" terminator 3148..3200 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 3696..3748 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" terminator 2893..3147 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" promoter 2199..2252 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" CDS complement(1517..2035) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" misc_feature 3749..3769 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" RBS 3369..3381 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" rep_origin 4330..5154 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator 2134..2194 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" misc_feature 1494..1494 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 3556..3684 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" CDS 2281..2883 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" misc_feature 3286..3360 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" promoter complement(2036..2133) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" primer_bind complement(3864..3883) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" RBS 2253..2280 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT 1561 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA AACGAAAAAC ATATTCTCAA 1621 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 1681 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG 1741 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT 1801 CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA 1861 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA 1921 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT 1981 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG 2041 CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT 2101 CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCctcggta ccaaattcca gaaaagaggc 2161 ctcccgaaag gggggccttt tttcgttttg gtccaatggc ggcgcgccat cgaatggtgc 2221 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcat gggggtgaat 2281 atggcacgta ccccgagccg tagcagcatt ggtagcctgc gtagtccgca tacccataaa 2341 gcaattctga ccagcaccat tgaaatcctg aaagaatgtg gttatagcgg tctgagcatt 2401 gaaagcgtgg cacgtcgcgc cggtgcaggc aaaccgacca tttatcgttg gtggaccaac 2461 aaagcagcac tgattgccga agtgtatgaa aatgaaatcg aacaggtacg taaatttccg 2521 gatttgggta gctttaaagc cgatctggat tttctgctgc ataatctgtg gaaagtttgg 2581 cgtgaaacca tttgtggtga agcatttcgt tgtgttattg cagaagcaca gttggaccct 2641 gtaaccctga cccaactgaa agatcagttt atggaacgtc gtcgtgagat accgaaaaaa 2701 ctggttgaag atgccattag caatggtgaa ctgccgaaag atatcaatcg tgaactgctg 2761 ctggatatga tttttggttt ttgttggtat cgcctgctga ccgaacagtt gaccgttgaa 2821 caggatattg aagaatttac cttcctgctg attaatggtg tttgtccggg tacacagtgt 2881 tgataaggat cctaattggt aacgaatcag acaattgacg gctcgaggga gtagcatagg 2941 gtttgcagaa tccctgcttc gtccatttga caggcacatt atgcatcgat gataagctgt 3001 caaacatgag cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg 3061 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt 3121 cgggctcatg agcaaatatt ttatctgcca attattgaag gcctccctaa cggggggcct 3181 ttttttgttt ctggtctccc gcttaacgat cgttggctgc gacgtacggt ggaatctgat 3241 tcgttaccaa ttgacatgat acgaaacgta ccgtatcgtt aaggtagctg tcaccggatg 3301 tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa 3361 tactagagtc acacaggaaa gtactagATG agctcctctt tacaattggc accagaaata 3421 gaaaagttgc ctcagagtga tatttactgg gaccccatcg tttctattac ggagactgga 3481 gtcgaagagg tttttgattt gactgtgcca ggactacgta actttgtcgc caatgacatc 3541 attgtacata acTCACTGCG TAGCAAAGAT CAGCAGGCAC AGATTATCAA AGTTGCACTG 3601 GGTAGCTTTT GGGCACTGCT GCGTGAACCG CCTCGTTTTC TGCTGTGTGA AGAAGCACAG 3661 ATTAAACAGG TGAAATCCTT CGAATAAtac tagagaacgc atgagAAAGC CCCCGGAAGA 3721 TCACCTTCCG GGGGCTTTtt tattgcgcta ctagtagcgg ccgctgcagg agtcactaag 3781 ggttagttag ttagattagc agaaagtcaa aagcctccga ccggaggctt ttgactaaaa 3841 cttcccttgg ggttatcatt ggggctcact caaaggcggt aatcagataa aaaaaatcct 3901 tagctttcgc taaggatgat ttctgctaga gatggaatag actggatgga ggcggataaa 3961 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 4021 ggagccggtg agcgtggatc tcgcggtatc attgcagcac tggggccaga tggtaagccc 4081 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 4141 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 4201 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 4261 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 4321 tcagacccct taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc 4381 tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac 4441 tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt 4501 tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc 4561 tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa 4621 ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc 4681 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga 4741 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca ggggaaacgc 4801 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg 4861 atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc 4921 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc 4981 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc 5041 ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca 5101 cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc gctagcgctg 5161 aggtctgcct cgtgaagaag gtgttgctga ctcataccag gcctgaatcg ccccatcatc 5221 cagccagaaa gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt 5281 gattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg 5341 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct 5401 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta 5461 cataaacagt aatacaaggg gtgtt //