LOCUS IBMc428 5470 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(176-213)-Ter ACCESSION IBMc428 VERSION IBMc428.1 FEATURES Location/Qualifiers misc_feature 3286..3360 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" promoter 2199..2252 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" misc_feature 1494..1494 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind complement(3849..3868) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 2281..2883 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 3391..3552 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 2893..3147 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" terminator 2134..2194 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" promoter 3220..3285 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" RBS 2253..2280 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" terminator 3681..3733 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter complement(2036..2133) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" misc_feature 3734..3754 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator 3148..3200 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" rep_origin 4315..5139 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(1517..2035) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 3556..3669 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" RBS 3369..3381 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT 1561 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA AACGAAAAAC ATATTCTCAA 1621 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 1681 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG 1741 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT 1801 CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA 1861 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA 1921 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT 1981 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG 2041 CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT 2101 CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCctcggta ccaaattcca gaaaagaggc 2161 ctcccgaaag gggggccttt tttcgttttg gtccaatggc ggcgcgccat cgaatggtgc 2221 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcat gggggtgaat 2281 atggcacgta ccccgagccg tagcagcatt ggtagcctgc gtagtccgca tacccataaa 2341 gcaattctga ccagcaccat tgaaatcctg aaagaatgtg gttatagcgg tctgagcatt 2401 gaaagcgtgg cacgtcgcgc cggtgcaggc aaaccgacca tttatcgttg gtggaccaac 2461 aaagcagcac tgattgccga agtgtatgaa aatgaaatcg aacaggtacg taaatttccg 2521 gatttgggta gctttaaagc cgatctggat tttctgctgc ataatctgtg gaaagtttgg 2581 cgtgaaacca tttgtggtga agcatttcgt tgtgttattg cagaagcaca gttggaccct 2641 gtaaccctga cccaactgaa agatcagttt atggaacgtc gtcgtgagat accgaaaaaa 2701 ctggttgaag atgccattag caatggtgaa ctgccgaaag atatcaatcg tgaactgctg 2761 ctggatatga tttttggttt ttgttggtat cgcctgctga ccgaacagtt gaccgttgaa 2821 caggatattg aagaatttac cttcctgctg attaatggtg tttgtccggg tacacagtgt 2881 tgataaggat cctaattggt aacgaatcag acaattgacg gctcgaggga gtagcatagg 2941 gtttgcagaa tccctgcttc gtccatttga caggcacatt atgcatcgat gataagctgt 3001 caaacatgag cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg 3061 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt 3121 cgggctcatg agcaaatatt ttatctgcca attattgaag gcctccctaa cggggggcct 3181 ttttttgttt ctggtctccc gcttaacgat cgttggctgc gacgtacggt ggaatctgat 3241 tcgttaccaa ttgacatgat acgaaacgta ccgtatcgtt aaggtagctg tcaccggatg 3301 tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa 3361 tactagagtc acacaggaaa gtactagATG agctcctctt tacaattggc accagaaata 3421 gaaaagttgc ctcagagtga tatttactgg gaccccatcg tttctattac ggagactgga 3481 gtcgaagagg tttttgattt gactgtgcca ggactacgta actttgtcgc caatgacatc 3541 attgtacata acTCACAGCA GGCACAGATT ATCAAAGTTG CACTGGGTAG CTTTTGGGCA 3601 CTGCTGCGTG AACCGCCTCG TTTTCTGCTG TGTGAAGAAG CACAGATTAA ACAGGTGAAA 3661 TCCTTCGAAT AAtactagag aacgcatgag AAAGCCCCCG GAAGATCACC TTCCGGGGGC 3721 TTTtttattg cgctactagt agcggccgct gcaggagtca ctaagggtta gttagttaga 3781 ttagcagaaa gtcaaaagcc tccgaccgga ggcttttgac taaaacttcc cttggggtta 3841 tcattggggc tcactcaaag gcggtaatca gataaaaaaa atccttagct ttcgctaagg 3901 atgatttctg ctagagatgg aatagactgg atggaggcgg ataaagttgc aggaccactt 3961 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 4021 ggatctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 4081 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 4141 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 4201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 4261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccttaata 4321 agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa 4381 accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt 4441 aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg 4501 catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt 4561 tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga 4621 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag 4681 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa 4741 aggcaggaac aggagagcgc acgagggagc cgccagggga aacgcctggt atctttatag 4801 tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg 4861 gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc 4921 ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga 4981 acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc 5041 tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct 5101 catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc tgcctcgtga 5161 agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag 5221 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg 5281 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc 5341 aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc 5401 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac 5461 aaggggtgtt //