LOCUS IBMc446 5021 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(160-236)-H6-Te r ACCESSION IBMc446 VERSION IBMc446.1 FEATURES Location/Qualifiers misc_feature 3285..3305 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" RBS 1635..1662 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" terminator 3232..3284 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter 1581..1634 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" rep_origin 3866..4690 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 3203..3220 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" terminator 1516..1576 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" CDS 2972..3202 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(160-236)" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 2831..2956 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP18" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" RBS 2816..2824 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" RBS 2765..2772 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" promoter 2602..2667 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" misc_feature 2746..2830 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" terminator 2530..2582 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" misc_feature 2668..2742 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" terminator 2275..2529 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" primer_bind complement(3400..3419) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 1663..2265 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct 1561 tttttcgttt tggtccaatg gcggcgcgcc atcgaatggt gcaaaacctt tcgcggtatg 1621 gcatgatagc gcccggaaga gagtcaattc atgggggtga atatggcacg taccccgagc 1681 cgtagcagca ttggtagcct gcgtagtccg catacccata aagcaattct gaccagcacc 1741 attgaaatcc tgaaagaatg tggttatagc ggtctgagca ttgaaagcgt ggcacgtcgc 1801 gccggtgcag gcaaaccgac catttatcgt tggtggacca acaaagcagc actgattgcc 1861 gaagtgtatg aaaatgaaat cgaacaggta cgtaaatttc cggatttggg tagctttaaa 1921 gccgatctgg attttctgct gcataatctg tggaaagttt ggcgtgaaac catttgtggt 1981 gaagcatttc gttgtgttat tgcagaagca cagttggacc ctgtaaccct gacccaactg 2041 aaagatcagt ttatggaacg tcgtcgtgag ataccgaaaa aactggttga agatgccatt 2101 agcaatggtg aactgccgaa agatatcaat cgtgaactgc tgctggatat gatttttggt 2161 ttttgttggt atcgcctgct gaccgaacag ttgaccgttg aacaggatat tgaagaattt 2221 accttcctgc tgattaatgg tgtttgtccg ggtacacagt gttgataagg atcctaattg 2281 gtaacgaatc agacaattga cggctcgagg gagtagcata gggtttgcag aatccctgct 2341 tcgtccattt gacaggcaca ttatgcatcg atgataagct gtcaaacatg agcagatcct 2401 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 2461 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcaaata 2521 ttttatctgc caattattga aggcctccct aacggggggc ctttttttgt ttctggtctc 2581 ccgcttaacg atcgttggct gcgacgtacg gtggaatctg attcgttacc aattgacatg 2641 atacgaaacg taccgtatcg ttaaggtagc tgtcaccgga tgtgctttcc ggtctgatga 2701 gtccgtgagg acgaaacagc ctctacaaat aattttgttt aataaGGGCC CAAGTTCACT 2761 TAAAAAGGAG ATCAACAATG AAAGCAATTT TCGTACTGAA ACATCTTAAT CATGCACAGG 2821 AGACTTTCTA atgagcatcg cggcgaccct ggagaacgat ctggcgcgtc tggaaaacga 2881 aaacgctcgt ctcgaaaaag acatcgcgaa cctggaacgt gacctggcga aactggagcg 2941 tgaagaagcg tacttcggag gttcaggtgg tggcgccctg aagggcgaga tcaagcagag 3001 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3061 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 3121 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 3181 cggcatggac gagctgtaca agcatcatca ccatcaccac taatactaga gaacgcatga 3241 gAAAGCCCCC GGAAGATCAC CTTCCGGGGG CTTTtttatt gcgctactag tagcggccgc 3301 tgcaggagtc actaagggtt agttagttag attagcagaa agtcaaaagc ctccgaccgg 3361 aggcttttga ctaaaacttc ccttggggtt atcattgggg ctcactcaaa ggcggtaatc 3421 agataaaaaa aatccttagc tttcgctaag gatgatttct gctagagatg gaatagactg 3481 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 3541 tattgctgat aaatctggag ccggtgagcg tggatctcgc ggtatcattg cagcactggg 3601 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 3661 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 3721 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 3781 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 3841 ttcgttccac tgagcgtcag accccttaat aagatgatct tcttgagatc gttttggtct 3901 gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt 3961 tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc agtcaccaaa 4021 acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact cctctaaatc 4081 aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg gactcaagac 4141 gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca 4201 gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga caaacgcggc 4261 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag 4321 ccgccagggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg 4381 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg 4441 cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg 4501 ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga 4561 ggaagcggaa tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct 4621 cctgccacat gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata 4681 cactccgcta gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct 4741 gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt 4801 aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg 4861 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccacg 4921 ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa 4981 taaaactgtc tgcttacata aacagtaata caaggggtgt t //