##gff-version 3
##sequence-region IBMc446 9 4690
IBMc446	.	biological_region	2746	2830	.	+	0	ID=annotation16;Name=BCD1
IBMc446	.	biological_region	1494	1515	.	+	0	ID=annotation8;Name=RFC10 Prefix (full)
IBMc446	.	ribosome_entry_site	2765	2772	.	+	0	ID=annotation13;Name=RBS for CDS 1
IBMc446	.	CDS	2972	3202	.	+	0	ID=annotation7;Name=mCherry*(160-236)
IBMc446	.	promoter	1581	1634	.	+	0	ID=annotation3;Name=PlacIQ
IBMc446	.	insulator	2668	2742	.	+	0	ID=annotation18;Name=RiboJ
IBMc446	.	terminator	1516	1576	.	+	0	ID=annotation6;Name=L3S2P21
IBMc446	.	biological_region	3285	3305	.	+	0	ID=annotation0;Name=RFC10 Suffix
IBMc446	.	ribosome_entry_site	1635	1662	.	+	0	ID=annotation1;Name=phl2
IBMc446	.	primer_binding_site	3400	3419	.	-	0	ID=annotation20;Name=VR
IBMc446	.	promoter	9	97	.	+	0	ID=annotation14;Name=tetR promoter
IBMc446	.	promoter	2602	2667	.	+	0	ID=annotation15;Name=PPhlF
IBMc446	.	CDS	1663	2265	.	+	0	ID=annotation21;Name=PhlF
IBMc446	.	CDS	3203	3220	.	+	0	ID=annotation5;Name=H6
IBMc446	.	terminator	3232	3284	.	+	0	ID=annotation2;Name=ECK120033736
IBMc446	.	terminator	2275	2529	.	+	0	ID=annotation19;Name=IOT
IBMc446	.	CDS	98	1291	.	+	0	ID=annotation12;Name=tetR
IBMc446	.	origin_of_replication	3866	4690	.	+	0	ID=annotation4;Name=p15A origin of replication
IBMc446	.	ribosome_entry_site	2816	2824	.	+	0	ID=annotation11;Name=RBS for CDS2
IBMc446	.	terminator	2530	2582	.	+	0	ID=annotation17;Name=L3S3P21
IBMc446	.	CDS	2831	2956	.	+	0	ID=annotation9;Name=SYNZIP18
IBMc446	.	primer_binding_site	1371	1390	.	+	0	ID=annotation10;Name=VF2
>IBMc446
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatg
gcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtg
aatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagca
ccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaacc
gaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaa
tttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccattt
gtggtgaagcatttcgttgtgttattgcagaagcacagttggaccctgtaaccctgacccaactgaaagatcagtttat
ggaacgtcgtcgtgagataccgaaaaaactggttgaagatgccattagcaatggtgaactgccgaaagatatcaatcgt
gaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagttgaccgttgaacaggatattg
aagaatttaccttcctgctgattaatggtgtttgtccgggtacacagtgttgataaggatcctaattggtaacgaatca
gacaattgacggctcgagggagtagcatagggtttgcagaatccctgcttcgtccatttgacaggcacattatgcatcg
atgataagctgtcaaacatgagcagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggtt
gctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcaaatattttatct
gccaattattgaaggcctccctaacggggggcctttttttgtttctggtctcccgcttaacgatcgttggctgcgacgt
acggtggaatctgattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggtagctgtcaccggatgtgct
ttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaataaGGGCCCAAGTTCACTTAAAA
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCATGCACAGGAGACTTTCTAatgagcatcgcggc
gaccctggagaacgatctggcgcgtctggaaaacgaaaacgctcgtctcgaaaaagacatcgcgaacctggaacgtgac
ctggcgaaactggagcgtgaagaagcgtacttcggaggttcaggtggtggcgccctgaagggcgagatcaagcagaggc
tgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccgg
cgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgcc
gagggccgccactccaccggcggcatggacgagctgtacaagcatcatcaccatcaccactaatactagagaacgcatg
agAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcactaaggg
ttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattg
gggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatag
actggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatc
tggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatc
tacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcatt
ggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggt
gaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaata
agatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtt
tttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcct
ttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgc
ttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcg
tgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataac
agcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatct
ttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatgg
aaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttc
gtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtat
cacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgcc
aacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcct
gaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgat
tttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagtt
cgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaa
caataaaactgtctgcttacataaacagtaatacaaggggtgtt