LOCUS IBMc447 5135 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(122-236)-H6-Te r ACCESSION IBMc447 VERSION IBMc447.1 FEATURES Location/Qualifiers terminator 2275..2529 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" misc_feature 3399..3419 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 1663..2265 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" terminator 3346..3398 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" RBS 1635..1662 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" CDS 3317..3334 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" promoter 1581..1634 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" CDS 2972..3316 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(122-236)" terminator 1516..1576 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" CDS 2831..2956 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP18" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" RBS 2816..2824 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" RBS 2765..2772 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" misc_feature 2668..2742 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" promoter 2602..2667 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" misc_feature 2746..2830 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" terminator 2530..2582 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" primer_bind complement(3514..3533) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" rep_origin 3980..4804 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct 1561 tttttcgttt tggtccaatg gcggcgcgcc atcgaatggt gcaaaacctt tcgcggtatg 1621 gcatgatagc gcccggaaga gagtcaattc atgggggtga atatggcacg taccccgagc 1681 cgtagcagca ttggtagcct gcgtagtccg catacccata aagcaattct gaccagcacc 1741 attgaaatcc tgaaagaatg tggttatagc ggtctgagca ttgaaagcgt ggcacgtcgc 1801 gccggtgcag gcaaaccgac catttatcgt tggtggacca acaaagcagc actgattgcc 1861 gaagtgtatg aaaatgaaat cgaacaggta cgtaaatttc cggatttggg tagctttaaa 1921 gccgatctgg attttctgct gcataatctg tggaaagttt ggcgtgaaac catttgtggt 1981 gaagcatttc gttgtgttat tgcagaagca cagttggacc ctgtaaccct gacccaactg 2041 aaagatcagt ttatggaacg tcgtcgtgag ataccgaaaa aactggttga agatgccatt 2101 agcaatggtg aactgccgaa agatatcaat cgtgaactgc tgctggatat gatttttggt 2161 ttttgttggt atcgcctgct gaccgaacag ttgaccgttg aacaggatat tgaagaattt 2221 accttcctgc tgattaatgg tgtttgtccg ggtacacagt gttgataagg atcctaattg 2281 gtaacgaatc agacaattga cggctcgagg gagtagcata gggtttgcag aatccctgct 2341 tcgtccattt gacaggcaca ttatgcatcg atgataagct gtcaaacatg agcagatcct 2401 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 2461 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcaaata 2521 ttttatctgc caattattga aggcctccct aacggggggc ctttttttgt ttctggtctc 2581 ccgcttaacg atcgttggct gcgacgtacg gtggaatctg attcgttacc aattgacatg 2641 atacgaaacg taccgtatcg ttaaggtagc tgtcaccgga tgtgctttcc ggtctgatga 2701 gtccgtgagg acgaaacagc ctctacaaat aattttgttt aataaGGGCC CAAGTTCACT 2761 TAAAAAGGAG ATCAACAATG AAAGCAATTT TCGTACTGAA ACATCTTAAT CATGCACAGG 2821 AGACTTTCTA atgagcatcg cggcgaccct ggagaacgat ctggcgcgtc tggaaaacga 2881 aaacgctcgt ctcgaaaaag acatcgcgaa cctggaacgt gacctggcga aactggagcg 2941 tgaagaagcg tacttcggag gttcaggtgg tgagttcatc tacaaggtga agctgcgcgg 3001 caccaacttc ccctccgacg gccccgtaat gcagaagAAA accatgggct gggaggcctc 3061 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 3121 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 3181 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 3241 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 3301 ggacgagctg tacaagcatc atcaccatca ccactaatac tagagaacgc atgagAAAGC 3361 CCCCGGAAGA TCACCTTCCG GGGGCTTTtt tattgcgcta ctagtagcgg ccgctgcagg 3421 agtcactaag ggttagttag ttagattagc agaaagtcaa aagcctccga ccggaggctt 3481 ttgactaaaa cttcccttgg ggttatcatt ggggctcact caaaggcggt aatcagataa 3541 aaaaaatcct tagctttcgc taaggatgat ttctgctaga gatggaatag actggatgga 3601 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 3661 tgataaatct ggagccggtg agcgtggatc tcgcggtatc attgcagcac tggggccaga 3721 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 3781 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 3841 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 3901 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 3961 ccactgagcg tcagacccct taataagatg atcttcttga gatcgttttg gtctgcgcgt 4021 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg 4081 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt 4141 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac 4201 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt 4261 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg 4321 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac 4381 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca 4441 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc 4501 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc 4561 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta 4621 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc 4681 ggaatatatc ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc 4741 acatgaagca cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc 4801 gctagcgctg aggtctgcct cgtgaagaag gtgttgctga ctcataccag gcctgaatcg 4861 ccccatcatc cagccagaaa gtgagggagc cacggttgat gagagctttg ttgtaggtgg 4921 accagttggt gattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat 4981 gcgtgatctg atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgt 5041 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac 5101 tgtctgctta cataaacagt aatacaaggg gtgtt //