LOCUS IBMc448 5030 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(157-236)-H6-Te r ACCESSION IBMc448 VERSION IBMc448.1 FEATURES Location/Qualifiers terminator 2530..2582 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" rep_origin 3875..4699 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator 2275..2529 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" misc_feature 3294..3314 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 1663..2265 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" terminator 3241..3293 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" RBS 1635..1662 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" CDS 3212..3229 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" promoter 1581..1634 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" CDS 2972..3211 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator 1516..1576 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" CDS 2831..2956 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP18" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" RBS 2816..2824 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" primer_bind complement(3409..3428) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" RBS 2765..2772 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" misc_feature 2668..2742 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" promoter 2602..2667 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" misc_feature 2746..2830 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct 1561 tttttcgttt tggtccaatg gcggcgcgcc atcgaatggt gcaaaacctt tcgcggtatg 1621 gcatgatagc gcccggaaga gagtcaattc atgggggtga atatggcacg taccccgagc 1681 cgtagcagca ttggtagcct gcgtagtccg catacccata aagcaattct gaccagcacc 1741 attgaaatcc tgaaagaatg tggttatagc ggtctgagca ttgaaagcgt ggcacgtcgc 1801 gccggtgcag gcaaaccgac catttatcgt tggtggacca acaaagcagc actgattgcc 1861 gaagtgtatg aaaatgaaat cgaacaggta cgtaaatttc cggatttggg tagctttaaa 1921 gccgatctgg attttctgct gcataatctg tggaaagttt ggcgtgaaac catttgtggt 1981 gaagcatttc gttgtgttat tgcagaagca cagttggacc ctgtaaccct gacccaactg 2041 aaagatcagt ttatggaacg tcgtcgtgag ataccgaaaa aactggttga agatgccatt 2101 agcaatggtg aactgccgaa agatatcaat cgtgaactgc tgctggatat gatttttggt 2161 ttttgttggt atcgcctgct gaccgaacag ttgaccgttg aacaggatat tgaagaattt 2221 accttcctgc tgattaatgg tgtttgtccg ggtacacagt gttgataagg atcctaattg 2281 gtaacgaatc agacaattga cggctcgagg gagtagcata gggtttgcag aatccctgct 2341 tcgtccattt gacaggcaca ttatgcatcg atgataagct gtcaaacatg agcagatcct 2401 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 2461 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcaaata 2521 ttttatctgc caattattga aggcctccct aacggggggc ctttttttgt ttctggtctc 2581 ccgcttaacg atcgttggct gcgacgtacg gtggaatctg attcgttacc aattgacatg 2641 atacgaaacg taccgtatcg ttaaggtagc tgtcaccgga tgtgctttcc ggtctgatga 2701 gtccgtgagg acgaaacagc ctctacaaat aattttgttt aataaGGGCC CAAGTTCACT 2761 TAAAAAGGAG ATCAACAATG AAAGCAATTT TCGTACTGAA ACATCTTAAT CATGCACAGG 2821 AGACTTTCTA atgagcatcg cggcgaccct ggagaacgat ctggcgcgtc tggaaaacga 2881 aaacgctcgt ctcgaaaaag acatcgcgaa cctggaacgt gacctggcga aactggagcg 2941 tgaagaagcg tacttcggag gttcaggtgg tcccgaggac ggcgccctga agggcgagat 3001 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 3061 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 3121 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 3181 ctccaccggc ggcatggacg agctgtacaa gcatcatcac catcaccact aatactagag 3241 aacgcatgag AAAGCCCCCG GAAGATCACC TTCCGGGGGC TTTtttattg cgctactagt 3301 agcggccgct gcaggagtca ctaagggtta gttagttaga ttagcagaaa gtcaaaagcc 3361 tccgaccgga ggcttttgac taaaacttcc cttggggtta tcattggggc tcactcaaag 3421 gcggtaatca gataaaaaaa atccttagct ttcgctaagg atgatttctg ctagagatgg 3481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3541 tggctggttt attgctgata aatctggagc cggtgagcgt ggatctcgcg gtatcattgc 3601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 3781 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 3841 acgtgagttt tcgttccact gagcgtcaga ccccttaata agatgatctt cttgagatcg 3901 ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt 3961 ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca 4021 gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc 4081 ctctaaatca attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg 4141 actcaagacg atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca 4201 tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac 4261 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc 4321 acgagggagc cgccagggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 4381 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg 4441 gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc 4501 tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc 4561 agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc 4621 cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa 4681 gccagtatac actccgctag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat 4741 accaggcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag 4801 ctttgttgta ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg 4861 cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac 4921 aaagccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat 4981 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt //