##gff-version 3
##sequence-region IBMc450 9 4639
IBMc450	.	ribosome_entry_site	2816	2824	.	+	0	ID=annotation20;Name=RBS for CDS2
IBMc450	.	terminator	1516	1576	.	+	0	ID=annotation17;Name=L3S2P21
IBMc450	.	ribosome_entry_site	2765	2772	.	+	0	ID=annotation0;Name=RBS for CDS 1
IBMc450	.	ribosome_entry_site	1635	1662	.	+	0	ID=annotation13;Name=phl2
IBMc450	.	promoter	1581	1634	.	+	0	ID=annotation15;Name=PlacIQ
IBMc450	.	promoter	2602	2667	.	+	0	ID=annotation4;Name=PPhlF
IBMc450	.	terminator	3181	3233	.	+	0	ID=annotation12;Name=ECK120033736
IBMc450	.	primer_binding_site	3349	3368	.	-	0	ID=annotation5;Name=VR
IBMc450	.	insulator	2668	2742	.	+	0	ID=annotation2;Name=RiboJ
IBMc450	.	biological_region	2746	2830	.	+	0	ID=annotation6;Name=BCD1
IBMc450	.	biological_region	3234	3254	.	+	0	ID=annotation10;Name=RFC10 Suffix
IBMc450	.	CDS	2972	3151	.	+	0	ID=annotation16;Name=mCherry*(177-236)
IBMc450	.	terminator	2275	2529	.	+	0	ID=annotation9;Name=IOT
IBMc450	.	CDS	98	1291	.	+	0	ID=annotation1;Name=tetR
IBMc450	.	promoter	9	97	.	+	0	ID=annotation3;Name=tetR promoter
IBMc450	.	CDS	2831	2956	.	+	0	ID=annotation18;Name=SYNZIP18
IBMc450	.	origin_of_replication	3815	4639	.	+	0	ID=annotation8;Name=p15A origin of replication
IBMc450	.	primer_binding_site	1371	1390	.	+	0	ID=annotation21;Name=VF2
IBMc450	.	CDS	3152	3169	.	+	0	ID=annotation14;Name=H6
IBMc450	.	biological_region	1494	1515	.	+	0	ID=annotation19;Name=RFC10 Prefix (full)
IBMc450	.	CDS	1663	2265	.	+	0	ID=annotation11;Name=PhlF
IBMc450	.	terminator	2530	2582	.	+	0	ID=annotation7;Name=L3S3P21
>IBMc450
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatg
gcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtg
aatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagca
ccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaacc
gaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaa
tttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccattt
gtggtgaagcatttcgttgtgttattgcagaagcacagttggaccctgtaaccctgacccaactgaaagatcagtttat
ggaacgtcgtcgtgagataccgaaaaaactggttgaagatgccattagcaatggtgaactgccgaaagatatcaatcgt
gaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagttgaccgttgaacaggatattg
aagaatttaccttcctgctgattaatggtgtttgtccgggtacacagtgttgataaggatcctaattggtaacgaatca
gacaattgacggctcgagggagtagcatagggtttgcagaatccctgcttcgtccatttgacaggcacattatgcatcg
atgataagctgtcaaacatgagcagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggtt
gctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcaaatattttatct
gccaattattgaaggcctccctaacggggggcctttttttgtttctggtctcccgcttaacgatcgttggctgcgacgt
acggtggaatctgattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggtagctgtcaccggatgtgct
ttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaataaGGGCCCAAGTTCACTTAAAA
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCATGCACAGGAGACTTTCTAatgagcatcgcggc
gaccctggagaacgatctggcgcgtctggaaaacgaaaacgctcgtctcgaaaaagacatcgcgaacctggaacgtgac
ctggcgaaactggagcgtgaagaagcgtacttcggaggttcaggtggtcactacgacgctgaggtcaagaccacctaca
aggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggacta
caccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagcatcatcac
catcaccactaatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactag
tagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttg
actaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgcta
aggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttc
cggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccaga
tggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgct
gagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaac
ttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttc
gttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaa
aacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttg
gaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatc
aattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggc
gcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcagg
cgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagg
gagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtg
atgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctgg
catcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagt
gagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaa
gcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaa
gaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagag
ctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgt
gatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacatt
gcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt