LOCUS IBMc454 4781 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR-HA-Ter ACCESSION IBMc454 VERSION IBMc454.1 FEATURES Location/Qualifiers rep_origin 3618..4442 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" misc_feature 1066..1087 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 2946..2972 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="HA tag" CDS complement(1088..1966) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" RBS 2286..2300 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind complement(3152..3171) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 2310..2327 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS 2328..2945 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="TetR" promoter 1994..2277 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" primer_bind 943..962 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 2984..3036 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" misc_feature 3037..3057 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 3..863 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR (BsaI-)" ORIGIN 1 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 61 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 121 cacgagtggg ttatatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 181 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 241 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 301 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 361 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 421 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 481 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 541 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 601 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 661 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtGGAt 721 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 781 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 841 cctcactgat taagcattgg taatactagc tccggcaaaa aaacgggcaa ggtgtcacca 901 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct gacgtctaag 961 aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga aggtgagcca 1021 gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct 1081 tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 1141 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 1201 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 1261 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 1321 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 1381 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 1441 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 1501 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 1561 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 1621 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 1681 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 1741 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 1801 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 1861 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 1921 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 1981 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct 2041 tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac 2101 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa 2161 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 2221 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccattac 2281 tagagattaa agaggagaaa tactagatgc atcatcacca tcaccactcc agattagata 2341 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 2401 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 2461 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 2521 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 2581 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 2641 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 2701 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 2761 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 2821 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 2881 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 2941 ggtccTATCC GTATGATGTT CCGGATTATG CCtgatacta gagaacgcat gagAAAGCCC 3001 CCGGAAGATC ACCTTCCGGG GGCTTTttta ttgcgctact agtagcggcc gctgcaggag 3061 tcactaaggg ttagttagtt agattagcag aaagtcaaaa gcctccgacc ggaggctttt 3121 gactaaaact tcccttgggg ttatcattgg ggctcactca aaggcggtaa tcagataaaa 3181 aaaatcctta gctttcgcta aggatgattt ctgctagaga tggaatagac tggatggagg 3241 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 3301 ataaatctgg agccggtgag cgtggatctc gcggtatcat tgcagcactg gggccagatg 3361 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 3421 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 3481 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 3541 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 3601 actgagcgtc agacccctta ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa 3661 tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag 3721 ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc 3781 tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca 3841 gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta 3901 ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag 3961 cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag 4021 cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg 4081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag 4141 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc 4201 tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag 4261 ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg 4321 aatatatcct gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac 4381 atgaagcact tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc 4441 tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc 4501 ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac 4561 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc 4621 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct 4681 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 4741 tctgcttaca taaacagtaa tacaaggggt gtttactaga g //