LOCUS IBMc456 5151 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-112)-5aa-SYNZIP17-BCD1-SYN ZIP18-5aa-TetR(113-207)-HA-Ter ACCESSION IBMc456 VERSION IBMc456.1 FEATURES Location/Qualifiers CDS 3316..3342 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="HA tag" misc_feature 1066..1087 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" RBS 2875..2883 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" RBS 2286..2300 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" CDS 2328..2660 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="TetR(2-112)" misc_feature 3407..3427 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" primer_bind 943..962 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2890..3015 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP18" misc_feature 2805..2889 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" CDS 3..863 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR (BsaI-)" CDS 3031..3315 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="TetR(113-207)" CDS complement(1088..1966) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" RBS 2824..2831 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" promoter 1994..2277 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" terminator 3354..3406 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" primer_bind complement(3522..3541) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" rep_origin 3988..4812 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 2676..2801 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP17" CDS 2310..2327 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" ORIGIN 1 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 61 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 121 cacgagtggg ttatatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 181 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 241 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 301 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 361 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 421 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 481 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 541 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 601 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 661 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtGGAt 721 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 781 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 841 cctcactgat taagcattgg taatactagc tccggcaaaa aaacgggcaa ggtgtcacca 901 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct gacgtctaag 961 aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga aggtgagcca 1021 gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct 1081 tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 1141 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 1201 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 1261 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 1321 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 1381 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 1441 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 1501 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 1561 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 1621 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 1681 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 1741 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 1801 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 1861 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 1921 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 1981 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct 2041 tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac 2101 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa 2161 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 2221 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccattac 2281 tagagattaa agaggagaaa tactagatgc atcatcacca tcaccactcc agattagata 2341 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 2401 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 2461 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 2521 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 2581 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 2641 cagaaaaaca gtatgaaact ggaggtggtg gatccaacga aaaagaagaa ctgaaatcca 2701 aaaaagcgga actgcgcaac cgtatcgaac agctgaaaca gaaacgtgaa caactgaagc 2761 agaaaatcgc gaacctgcgt aaagaaatcg aagcttacaa ataaGGGCCC AAGTTCACTT 2821 AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA CATCTTAATC ATGCACAGGA 2881 GACTTTCTAa tgagcatcgc ggcgaccctg gagaacgatc tggcgcgtct ggaaaacgaa 2941 aacgctcgtc tcgaaaaaga catcgcgaac ctggaacgtg acctggcgaa actggagcgt 3001 gaagaagcgt acttcggagg ttcaggtggt ctcgaaaatc aattagcctt tttatgccaa 3061 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 3121 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 3181 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 3241 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 3301 tgtgaaagtg ggtccTATCC GTATGATGTT CCGGATTATG CCtgatacta gagaacgcat 3361 gagAAAGCCC CCGGAAGATC ACCTTCCGGG GGCTTTttta ttgcgctact agtagcggcc 3421 gctgcaggag tcactaaggg ttagttagtt agattagcag aaagtcaaaa gcctccgacc 3481 ggaggctttt gactaaaact tcccttgggg ttatcattgg ggctcactca aaggcggtaa 3541 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctgctagaga tggaatagac 3601 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 3661 tttattgctg ataaatctgg agccggtgag cgtggatctc gcggtatcat tgcagcactg 3721 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 3781 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 3841 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 3901 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 3961 ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga tcgttttggt 4021 ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag 4081 gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca 4141 aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa 4201 tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag 4261 acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc 4321 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg 4381 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg 4441 agccgccagg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt 4501 tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc 4561 cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc 4621 cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc 4681 gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc agcctttttt 4741 ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag taagccagta 4801 tacactccgc tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc 4861 ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt 4921 gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc 4981 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca 5041 cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac 5101 aataaaactg tctgcttaca taaacagtaa tacaaggggt gtttactaga g //