##gff-version 3
##sequence-region IBMc456 3 4812
IBMc456	.	CDS	2676	2801	.	+	0	ID=annotation17;Name=SYNZIP17
IBMc456	.	CDS	2890	3015	.	+	0	ID=annotation7;Name=SYNZIP18
IBMc456	.	primer_binding_site	3522	3541	.	-	0	ID=annotation15;Name=VR
IBMc456	.	primer_binding_site	943	962	.	+	0	ID=annotation6;Name=VF2
IBMc456	.	biological_region	3407	3427	.	+	0	ID=annotation5;Name=RFC10 Suffix
IBMc456	.	CDS	3	863	.	+	0	ID=annotation9;Name=ampR (BsaI-)
IBMc456	.	promoter	1994	2277	.	+	0	ID=annotation13;Name=PBAD(SapI-)
IBMc456	.	CDS	3316	3342	.	+	0	ID=annotation0;Name=HA tag
IBMc456	.	CDS	1088	1966	.	-	0	ID=annotation11;Name=araC
IBMc456	.	ribosome_entry_site	2824	2831	.	+	0	ID=annotation12;Name=RBS for CDS 1
IBMc456	.	CDS	2328	2660	.	+	0	ID=annotation4;Name=TetR(2-112)
IBMc456	.	biological_region	2805	2889	.	+	0	ID=annotation8;Name=BCD1
IBMc456	.	ribosome_entry_site	2875	2883	.	+	0	ID=annotation2;Name=RBS for CDS2
IBMc456	.	origin_of_replication	3988	4812	.	+	0	ID=annotation16;Name=p15A origin of replication
IBMc456	.	terminator	3354	3406	.	+	0	ID=annotation14;Name=ECK120033736
IBMc456	.	biological_region	1066	1087	.	+	0	ID=annotation1;Name=RFC10 Prefix (full)
IBMc456	.	CDS	3031	3315	.	+	0	ID=annotation10;Name=TetR(113-207)
IBMc456	.	CDS	2310	2327	.	+	0	ID=annotation18;Name=H6
IBMc456	.	ribosome_entry_site	2286	2300	.	+	0	ID=annotation3;Name=BBa_B0030
>IBMc456
gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccaga
aacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggt
aagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtat
tatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcacc
agtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacact
gcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaa
ctcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaat
ggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggag
gcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtg
agcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggg
gagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactag
ctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccac
ctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagtt
gattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggct
acatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgaga
aatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgc
ctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgac
ggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaag
cctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaag
cagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaa
tgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagt
aggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcc
tggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatg
gtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcg
ttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcat
actcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtc
tcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgta
acaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatt
tttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaa
gaggagaaatactagatgcatcatcaccatcaccactccagattagataaaagtaaagtgattaacagcgcattagagc
tgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgta
ttggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgc
cctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcg
atggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactggaggtggtggatccaacgaaaaaga
agaactgaaatccaaaaaagcggaactgcgcaaccgtatcgaacagctgaaacagaaacgtgaacaactgaagcagaaa
atcgcgaacctgcgtaaagaaatcgaagcttacaaataaGGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGC
AATTTTCGTACTGAAACATCTTAATCATGCACAGGAGACTTTCTAatgagcatcgcggcgaccctggagaacgatctgg
cgcgtctggaaaacgaaaacgctcgtctcgaaaaagacatcgcgaacctggaacgtgacctggcgaaactggagcgtga
agaagcgtacttcggaggttcaggtggtctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaat
gcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaag
aagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgc
agagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccTAT
CCGTATGATGTTCCGGATTATGCCtgatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTt
ttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctcc
gaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaat
ccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttc
tgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgc
agcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacga
aatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatacttt
agattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatccc
ttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgta
atctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaacc
gaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagac
taactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgata
gttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccg
gaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaaca
ggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttga
gcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgt
taagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccga
gcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagcctttttt
ctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctga
ggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagc
cacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgtt
gtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaat
ctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaa
ggggtgtttactagag