##gff-version 3
##sequence-region IBMc457 3 4812
IBMc457	.	origin_of_replication	3988	4812	.	+	0	ID=annotation2;Name=p15A origin of replication
IBMc457	.	CDS	3052	3177	.	+	0	ID=annotation18;Name=SYNZIP18
IBMc457	.	CDS	1088	1966	.	-	0	ID=annotation7;Name=araC
IBMc457	.	CDS	3316	3342	.	+	0	ID=annotation8;Name=HA tag
IBMc457	.	CDS	3	863	.	+	0	ID=annotation12;Name=ampR (BsaI-)
IBMc457	.	terminator	3354	3406	.	+	0	ID=annotation17;Name=ECK120033736
IBMc457	.	CDS	2328	2822	.	+	0	ID=annotation3;Name=TetR(2-166)
IBMc457	.	ribosome_entry_site	3037	3045	.	+	0	ID=annotation4;Name=RBS for CDS2
IBMc457	.	primer_binding_site	943	962	.	+	0	ID=annotation15;Name=VF2
IBMc457	.	CDS	3193	3315	.	+	0	ID=annotation14;Name=TetR(167-207)
IBMc457	.	ribosome_entry_site	2986	2993	.	+	0	ID=annotation9;Name=RBS for CDS 1
IBMc457	.	biological_region	2967	3051	.	+	0	ID=annotation16;Name=BCD1
IBMc457	.	CDS	2838	2963	.	+	0	ID=annotation6;Name=SYNZIP17
IBMc457	.	CDS	2310	2327	.	+	0	ID=annotation1;Name=H6
IBMc457	.	biological_region	1066	1087	.	+	0	ID=annotation10;Name=RFC10 Prefix (full)
IBMc457	.	promoter	1994	2277	.	+	0	ID=annotation5;Name=PBAD(SapI-)
IBMc457	.	ribosome_entry_site	2286	2300	.	+	0	ID=annotation0;Name=BBa_B0030
IBMc457	.	biological_region	3407	3427	.	+	0	ID=annotation11;Name=RFC10 Suffix
IBMc457	.	primer_binding_site	3522	3541	.	-	0	ID=annotation13;Name=VR
>IBMc457
gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccaga
aacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggt
aagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtat
tatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcacc
agtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacact
gcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaa
ctcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaat
ggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggag
gcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtg
agcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggg
gagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactag
ctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccac
ctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagtt
gattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggct
acatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgaga
aatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgc
ctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgac
ggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaag
cctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaag
cagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaa
tgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagt
aggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcc
tggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatg
gtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcg
ttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcat
actcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtc
tcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgta
acaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatt
tttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaa
gaggagaaatactagatgcatcatcaccatcaccactccagattagataaaagtaaagtgattaacagcgcattagagc
tgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgta
ttggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgc
cctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcg
atggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatg
ccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaa
gatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgggaggtggtggatccaacgaaa
aagaagaactgaaatccaaaaaagcggaactgcgcaaccgtatcgaacagctgaaacagaaacgtgaacaactgaagca
gaaaatcgcgaacctgcgtaaagaaatcgaagcttacaaataaGGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGA
AAGCAATTTTCGTACTGAAACATCTTAATCATGCACAGGAGACTTTCTAatgagcatcgcggcgaccctggagaacgat
ctggcgcgtctggaaaacgaaaacgctcgtctcgaaaaagacatcgcgaacctggaacgtgacctggcgaaactggagc
gtgaagaagcgtacttcggaggttcaggtggtccgccattattacgacaagctatcgaattatttgatcaccaaggtgc
agagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccTAT
CCGTATGATGTTCCGGATTATGCCtgatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTt
ttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctcc
gaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaat
ccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttc
tgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgc
agcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacga
aatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatacttt
agattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatccc
ttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgta
atctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaacc
gaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagac
taactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgata
gttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccg
gaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaaca
ggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttga
gcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgt
taagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccga
gcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagcctttttt
ctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctga
ggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagc
cacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgtt
gtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaat
ctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaa
ggggtgtttactagag