LOCUS IBMc460 4689 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI ACCESSION IBMc460 VERSION IBMc460.1 FEATURES Location/Qualifiers CDS complement(1595..2254) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" RBS 2472..2499 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" terminator 3112..3366 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" terminator 3367..3419 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" misc_feature 3732..3752 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator complement(1510..1566) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" primer_bind complement(3810..3829) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 3505..3579 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" primer_bind 982..1001 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 2353..2413 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" CDS 1137..1400 /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="gp41-1N" rep_origin complement(3916..4530) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" misc_feature 1100..1121 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" terminator 1412..1501 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 3607..3717 /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="gp41-1C" promoter complement(2255..2352) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" promoter 3439..3504 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" RBS 3588..3600 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS complement(1..861) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR (rev)" promoter 2418..2471 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" CDS 2500..3102 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatata acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 1021 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 1081 tcgctaagga tgatttctgg aattcgcggc cgcttctaga gGAAGACGCT GGATATTGTC 1141 TGGATCTGAA AACCCAGGTT CAGACACCGC AGGGTATGAA AGAAATTTCA AATATTCAGG 1201 TGGGTGATCT GGTTCTGAGC AATACCGGTT ATAATGAAGT GCTGAATGTG TTTCCGAAAA 1261 GCAAAAAAAA AAGCTATAAA ATCACCCTGG AAGATGGCAA AGAAATCATT TGTAGCGAAG 1321 AACACCTGTT TCCGACCCAG ACCGGTGAAA TGAATATTAG CGGTGGTCTG AAAGAAGGTA 1381 TGTGCCTGTA TGTTAAAGAA TAATACTAGA Gttgagaaga gaaaagaaaa ccgccgatcc 1441 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga 1501 aTACTAGAGc ctttggtcgA AAAAAAAAGC CCGCACTGTC AGGTGCGGGC TTTTTTctgt 1561 gtttccTACT AGAGCAAGCG AGCTCGATAT CAAATTACGC CCCGCCCTGC CACTCATCGC 1621 AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT 1681 GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG 1741 TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC 1801 TCACCCAGGG ATTGGCTGAA ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG 1861 CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT 1921 CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT 1981 AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGAAATT 2041 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT 2101 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG 2161 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT 2221 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC 2281 TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC ATTATGGTGA AAGTTGGAAC 2341 CTCTTACGTG CCctcggtac caaattccag aaaagaggcc tcccgaaagg ggggcctttt 2401 ttcgttttgg tccaatggcg gcgcgccatc gaatggtgca aaacctttcg cggtatggca 2461 tgatagcgcc cggaagagag tcaattcatg ggggtgaata tggcacgtac cccgagccgt 2521 agcagcattg gtagcctgcg tagtccgcat acccataaag caattctgac cagcaccatt 2581 gaaatcctga aagaatgtgg ttatagcggt ctgagcattg aaagcgtggc acgtcgcgcc 2641 ggtgcaggca aaccgaccat ttatcgttgg tggaccaaca aagcagcact gattgccgaa 2701 gtgtatgaaa atgaaatcga acaggtacgt aaatttccgg atttgggtag ctttaaagcc 2761 gatctggatt ttctgctgca taatctgtgg aaagtttggc gtgaaaccat ttgtggtgaa 2821 gcatttcgtt gtgttattgc agaagcacag ttggaccctg taaccctgac ccaactgaaa 2881 gatcagttta tggaacgtcg tcgtgagata ccgaaaaaac tggttgaaga tgccattagc 2941 aatggtgaac tgccgaaaga tatcaatcgt gaactgctgc tggatatgat ttttggtttt 3001 tgttggtatc gcctgctgac cgaacagttg accgttgaac aggatattga agaatttacc 3061 ttcctgctga ttaatggtgt ttgtccgggt acacagtgtt gataaggatc ctaattggta 3121 acgaatcaga caattgacgg ctcgagggag tagcataggg tttgcagaat ccctgcttcg 3181 tccatttgac aggcacatta tgcatcgatg ataagctgtc aaacatgagc agatcctcta 3241 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 3301 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcaaatattt 3361 tatctgccaa ttattgaagg cctccctaac ggggggcctt tttttgtttc tggtctcccg 3421 cttaacgatc gttggctgcg acgtacggtg gaatctgatt cgttaccaat tgacatgata 3481 cgaaacgtac cgtatcgtta aggtagctgt caccggatgt gctttccggt ctgatgagtc 3541 cgtgaggacg aaacagcctc tacaaataat tttgtttaat actagagtca cacaggaaag 3601 tactagATGA TGCTGAAAAA AATCCTGAAA ATCGAGGAAC TGGATGAACG CGAACTGATT 3661 GATATTGAAG TTAGCGGTAA CCACCTGTTC TATGCCAATG ATATTCTGAC CCATAATTCG 3721 TCAAGAAGAG Ctactagtag cggccgctgc aggcttcctc gctcactgac tcgctgcgct 3781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 3841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 3901 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc 3961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 4021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 4081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 4141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 4201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 4261 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 4321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 4381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 4441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 4501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 4561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 4621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 4681 ctgacagtt //