LOCUS IBMc474 3933 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB1A3-PBAD(SapI-)-B30-mCherry*(1-191)-Ter ACCESSION IBMc474 VERSION IBMc474.1 FEATURES Location/Qualifiers promoter 2024..2307 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS complement(1..861) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR" CDS 2337..2909 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-191)" terminator 2934..2990 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" CDS complement(1118..1996) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" rep_origin complement(3160..3774) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" RBS 2316..2330 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind 982..1001 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" primer_bind complement(3054..3073) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatata acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 1021 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 1081 tcgctaagga tgatttctgg aattcgagct cggtacctta tgacaacttg acggctacat 1141 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt 1201 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga 1261 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc 1321 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca 1381 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga 1441 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg 1501 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc 1561 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt 1621 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt 1681 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg 1741 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc 1801 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa 1861 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa 1921 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat 1981 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat 2041 attgcatcag acattgccgt cactgcgtct tttactggcA cttctcgcta accaaaccgg 2101 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc 2161 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca 2221 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt 2281 tatcgcaact ctctactgtt tctccattac tagagattaa agaggagaaa tactagatgg 2341 tgagcaaggg cgaagaaGAT aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 2401 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2461 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 2521 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 2581 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 2641 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg 2701 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 2761 agaagAAAac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 2821 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 2881 tcaagaccac ctacaaggcc aagaagccct aaGGGCCCAA GTTCACTTAA AAACTCGGTA 2941 CCAAAGACGA ACAATAAGAC GCTGAAAAGC GTCTTTTTTC GTTTTGGTCC ctgcaggctt 3001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 3061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 3121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca 3181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 3241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 3301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 3361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 3421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 3481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 3541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 3601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 3661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 3721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 3781 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 3841 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 3901 aaagtatata tgagtaaact tggtctgaca gtt //