LOCUS IBMc480 4029 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB1A3-PBAD(SapI-)-B30-mCherry*(1-176)-5aa-SYNZIP17-Ter ACCESSION IBMc480 VERSION IBMc480.1 FEATURES Location/Qualifiers primer_bind 982..1001 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2880..3005 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP17" CDS complement(1118..1996) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" rep_origin complement(3256..3870) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" CDS complement(1..861) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR" CDS 2340..2864 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-176)" primer_bind complement(3150..3169) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter 2024..2307 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" RBS 2316..2330 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator 3030..3086 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatata acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 1021 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 1081 tcgctaagga tgatttctgg aattcgagct cggtacctta tgacaacttg acggctacat 1141 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt 1201 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga 1261 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc 1321 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca 1381 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga 1441 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg 1501 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc 1561 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt 1621 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt 1681 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg 1741 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc 1801 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa 1861 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa 1921 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat 1981 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat 2041 attgcatcag acattgccgt cactgcgtct tttactggcA cttctcgcta accaaaccgg 2101 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc 2161 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca 2221 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt 2281 tatcgcaact ctctactgtt tctccattac tagagattaa agaggagaaa tactagatgg 2341 tgagcaaggg cgaagaaGAT aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 2401 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2461 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 2521 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 2581 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 2641 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg 2701 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 2761 agaagAAAac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 2821 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggcggaggt ggtggatcca 2881 acgaaaaaga agaactgaaa tccaaaaaag cggaactgcg caaccgtatc gaacagctga 2941 aacagaaacg tgaacaactg aagcagaaaa tcgcgaacct gcgtaaagaa atcgaagctt 3001 acaaataaGG GCCCAAGTTC ACTTAAAAAC TCGGTACCAA AGACGAACAA TAAGACGCTG 3061 AAAAGCGTCT TTTTTCGTTT TGGTCCctgc aggcttcctc gctcactgac tcgctgcgct 3121 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 3181 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 3241 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc 3301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 3361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 3421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 3481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 3541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 3601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 3661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 3721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 3781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 3841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 3901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 3961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 4021 ctgacagtt //