Intein_assisted_Bisection_Mapping
type: Collection
persistent identity: Intein assisted Bisection Mapping collection
display id: Intein_assisted_Bisection_Mapping_collection
version: 1
description: Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
creator: Trevor Yuan Heng Ho
created: 2020-11-23T00:02:29.914Z
modified: 2020-11-23T00:08:06
mutable description: Plasmid maps and sequences for constructs used in the study "Intein-assisted bisection mapping systematically splits proteins for Boolean logic and inducibility engineering"
owned by: trevorho
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387_seq/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412_seq/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470_seq/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329_seq/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406_seq/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458_seq/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393_seq/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176_seq/1
member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
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member: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078_seq/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
type: ComponentDefinition
persistent identity: IBMc405
display id: IBMc405
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-54)-KLG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-54)-KLG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: araC
sequence annotation: pSEVA PS2 Rev
sequence annotation: lambda t0 terminator
sequence annotation: cat promoter
sequence annotation: M86N
sequence annotation: BBa_B0030
sequence annotation: ECK120033737
sequence annotation: RFC10 Prefix (full)
sequence annotation: trfA CDS (replication protein)
sequence annotation: PBAD(SapI-)
sequence annotation: cat (BsmBI-)
sequence annotation: SrpR
sequence annotation: ECK120029600
sequence annotation: pSEVA PS1 Fwd
sequence annotation: cat
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405_seq/1

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 78
end: 956
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 2256
end: 2272
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 2202
end: 2296
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 2349
end: 2446
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 1468
end: 1767
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 1276
end: 1290
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 1877
end: 1933
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 57
end: 77
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 3424
end: 4572
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 984
end: 1267
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 1962
end: 2102
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 1297
end: 1458
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 1779
end: 1868
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 5613
end: 5630
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405/1
start: 2447
end: 3106
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
type: ComponentDefinition
persistent identity: IBMc333
display id: IBMc333
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR(1-22)-BsaI-BsaI-SrpR(192-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR(1-22)-BsaI-BsaI-SrpR(192-213)-Ter Expression vector to generate ORF insertion library in IBM on SrpR
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: SrpR(1-22)
sequence annotation: araC
sequence annotation: VF2
sequence annotation: p15A origin of replication
sequence annotation: spacing sequence
sequence annotation: ECK120033736
sequence annotation: tetR
sequence annotation: SrpR(192-213)
sequence annotation: RFC10 Suffix
sequence annotation: VR
sequence annotation: tetR promoter
sequence annotation: RFC10 Prefix (full)
sequence annotation: BBa_B0030
sequence annotation: PBAD(SapI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333_seq/1

SrpR(1-22)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR(1-22)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 2735
end: 2802
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 1516
end: 2394
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 1371
end: 1390
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 3545
end: 4369
orientation: inline

spacing sequence
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: spacing sequence
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 2810
end: 2825
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 2911
end: 2963
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 98
end: 1291
orientation: inline

SrpR(192-213)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR(192-213)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 2833
end: 2899
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 2964
end: 2984
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 3079
end: 3098
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 9
end: 97
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 1494
end: 1515
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 2714
end: 2728
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333/1
start: 2422
end: 2705
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
type: ComponentDefinition
persistent identity: IBMc459
display id: IBMc459
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B32-bla-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B32-bla-Ter beta-lactamase expression plasmid. Activity reference in IBM experimenton beta-lactamase
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: p15A origin of replication
sequence annotation: tetR promoter
sequence annotation: VF2
sequence annotation: tetR
sequence annotation: araC
sequence annotation: TEM-1 bla
sequence annotation: VR
sequence annotation: RFC10 Prefix (full)
sequence annotation: PBAD(SapI-)
sequence annotation: RFC10 Suffix
sequence annotation: BBa_B0032
sequence annotation: ECK120033736
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459_seq/1

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 4236
end: 5060
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 9
end: 97
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 1371
end: 1390
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 98
end: 1291
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 1516
end: 2394
orientation: reverseComplement

TEM-1 bla
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: TEM-1 bla
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 2733
end: 3590
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 3770
end: 3789
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 1494
end: 1515
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 2422
end: 2705
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 3655
end: 3675
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 2714
end: 2726
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459/1
start: 3602
end: 3654
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
type: ComponentDefinition
persistent identity: IBMc448
display id: IBMc448
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(157-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(157-236)-H6-Ter Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: L3S3P21
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: PPhlF
sequence annotation: VF2
sequence annotation: ECK120033736
sequence annotation: IOT
sequence annotation: RBS for CDS 1
sequence annotation: mCherry*
sequence annotation: p15A origin of replication
sequence annotation: tetR
sequence annotation: RBS for CDS2
sequence annotation: PhlF
sequence annotation: PlacIQ
sequence annotation: RiboJ
sequence annotation: phl2
sequence annotation: L3S2P21
sequence annotation: VR
sequence annotation: SYNZIP18
sequence annotation: BCD1
sequence annotation: tetR promoter
sequence annotation: H6
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448_seq/1

L3S3P21
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 2530
end: 2582
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 1494
end: 1515
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 3294
end: 3314
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 2602
end: 2667
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation15/range/1
type: Range
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ECK120033736
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation5/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3241
end: 3293
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation2/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2275
end: 2529
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation16/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2765
end: 2772
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation9/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2972
end: 3211
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
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role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation1/range/1

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type: Range
persistent identity: range
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version: 1
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start: 3875
end: 4699
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation17/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 98
end: 1291
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation13/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2816
end: 2824
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation4/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 1663
end: 2265
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation8/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 1581
end: 1634
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 2668
end: 2742
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation6/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 1635
end: 1662
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 1516
end: 1576
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 3409
end: 3428
orientation: reverseComplement

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 2831
end: 2956
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 2746
end: 2830
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 9
end: 97
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448/1
start: 3212
end: 3229
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
type: ComponentDefinition
persistent identity: IBMc399
display id: IBMc399
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-188)-KNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(189-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-188)-KNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(189-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat promoter
sequence annotation: BBa_B0030
sequence annotation: Plux2
sequence annotation: cat (BsmBI-)
sequence annotation: araC
sequence annotation: BBa_J23117
sequence annotation: H6
sequence annotation: mCherry*
sequence annotation: PBAD(SapI-)
sequence annotation: BBa_B0012
sequence annotation: ECK120033736
sequence annotation: ECK120029600
sequence annotation: RFC10 Suffix
sequence annotation: L3S2P55
sequence annotation: tetR
sequence annotation: VF2
sequence annotation: M86C
sequence annotation: luxR
sequence annotation: ECK120033737
sequence annotation: VR
sequence annotation: M86N
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Prefix (full)
sequence annotation: BBa_B0032
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399_seq/1

cat promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 4462
end: 4559
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 2714
end: 2728
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 5527
end: 5580
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 3802
end: 4461
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 1516
end: 2394
orientation: reverseComplement

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 5443
end: 5477
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 5920
end: 5937
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 2735
end: 3303
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 2422
end: 2705
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 5478
end: 5518
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 5949
end: 6001
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation16/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3619
end: 3708
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation4/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 6002
end: 6022
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation0/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 5420
end: 5434
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation5/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 4568
end: 4624
orientation: reverseComplement

tetR
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation25/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 98
end: 1291
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation15/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 1371
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orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation14/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 5611
end: 5772
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation2/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 4658
end: 5413
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 3717
end: 3773
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 6117
end: 6136
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 3308
end: 3607
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 6583
end: 7407
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 5776
end: 5919
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 1494
end: 1515
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 5589
end: 5601
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399/1
start: 9
end: 97
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
type: ComponentDefinition
persistent identity: IBMc188
display id: IBMc188
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-2aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-2aa-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-2aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-2aa-ECF20(102-193)-T Expression plasmid for ECF20 spit at 101/102 fused with acVHH via 2 aa linker
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR
sequence annotation: ECK120033737
sequence annotation: RFC10 Suffix
sequence annotation: araC
sequence annotation: PBAD(SapI-)
sequence annotation: ECF20 102-193
sequence annotation: tetR promoter
sequence annotation: L3S2P55
sequence annotation: acVHH
sequence annotation: cat promoter
sequence annotation: BCD22
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Prefix (full)
sequence annotation: RiboJ
sequence annotation: ECF20 1-101
sequence annotation: VR
sequence annotation: RBS for 2nd CDS
sequence annotation: cat (BsmBI-)
sequence annotation: GG linker
sequence annotation: VF2
sequence annotation: BBa_B0033
sequence annotation: RBS for CDS 1
sequence annotation: ECK120029600
sequence annotation: ECK120033736
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188_seq/1

tetR
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 98
end: 1291
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 3506
end: 3562
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 5588
end: 5608
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 1516
end: 2394
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 2422
end: 2705
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 5248
end: 5523
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 9
end: 97
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 4357
end: 4413
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 3040
end: 3396
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 4251
end: 4348
orientation: reverseComplement

BCD22
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: BCD22
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 4797
end: 4884
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 6169
end: 6993
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 1494
end: 1515
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 4885
end: 5241
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 4722
end: 4796
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 2731
end: 3033
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 5703
end: 5722
orientation: reverseComplement

RBS for 2nd CDS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: RBS for 2nd CDS
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 4867
end: 4875
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 3591
end: 4250
orientation: reverseComplement

GG linker
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: GG linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 3034
end: 3039
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 1371
end: 1390
orientation: inline

GG linker
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: GG linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 5242
end: 5247
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 2714
end: 2724
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 4816
end: 4823
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 4433
end: 4716
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 3408
end: 3497
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188/1
start: 5535
end: 5587
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
type: ComponentDefinition
persistent identity: IBMc228
display id: IBMc228
version: 1
description: pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI Staging plasmid for IBM on TetR
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1C3 Origin of replication: pMB1 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat (BsmBI-)
sequence annotation: VR
sequence annotation: rep (pMB1)
sequence annotation: TetR
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence annotation: VF2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228_seq/1

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
start: 1
end: 660
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
start: 1589
end: 1608
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
start: 1695
end: 2309
orientation: reverseComplement

TetR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
start: 919
end: 1427
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
start: 1435
end: 1455
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
start: 890
end: 911
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228/1
start: 772
end: 791
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
type: ComponentDefinition
persistent identity: IBMc413
display id: IBMc413
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-170)-LRG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-170)-LRG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat
sequence annotation: ECK120033737
sequence annotation: RFC10 Prefix (full)
sequence annotation: trfA CDS (replication protein)
sequence annotation: pSEVA PS2 Rev
sequence annotation: SrpR
sequence annotation: M86N
sequence annotation: PBAD(SapI-)
sequence annotation: pSEVA PS1 Fwd
sequence annotation: BBa_B0030
sequence annotation: araC
sequence annotation: lambda t0 terminator
sequence annotation: ECK120029600
sequence annotation: cat promoter
sequence annotation: cat (BsmBI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413_seq/1

cat
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 2795
end: 3454
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 2225
end: 2281
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 57
end: 77
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 3772
end: 4920
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 2604
end: 2620
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 1297
end: 1806
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 1816
end: 2115
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 984
end: 1267
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 5961
end: 5978
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 1276
end: 1290
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 78
end: 956
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 2550
end: 2644
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 2127
end: 2216
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 2697
end: 2794
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413/1
start: 2310
end: 2450
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
type: ComponentDefinition
persistent identity: IBMc378
display id: IBMc378
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry*(193-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry*(193-236)-H6-T Expression plasmid for mCherry split at 192/193 split by M86 intein split at 17/18 by acVHH
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: H6
sequence annotation: ECK120033736
sequence annotation: tetR promoter
sequence annotation: p15A origin of replication
sequence annotation: cat (BsmBI-)
sequence annotation: ECK120029600
sequence annotation: RiboJ
sequence annotation: RFC10 Suffix
sequence annotation: RBS for CDS 1
sequence annotation: M86(18-154)
sequence annotation: PBAD(SapI-)
sequence annotation: cat promoter
sequence annotation: VR
sequence annotation: RBS for CDS2
sequence annotation: L3S2P55
sequence annotation: mCherry*(193-236)
sequence annotation: acVHH
sequence annotation: M86(1-17)
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence annotation: mCherry*(1-192)
sequence annotation: araC
sequence annotation: 2x(GGGGS) linker
sequence annotation: ECK120033737
sequence annotation: VF2
sequence annotation: BBa_B0030
sequence annotation: BCD1
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378_seq/1

H6
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 6171
end: 6188
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 6200
end: 6252
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 1
end: 89
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 6834
end: 7658
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 3944
end: 4603
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 3761
end: 3850
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 5075
end: 5149
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 6253
end: 6273
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation27
display id: annotation27
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation27/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation27/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 5169
end: 5176
orientation: inline

M86(18-154)
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(18-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 5625
end: 6035
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 2414
end: 2697
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation28
display id: annotation28
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation28/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation28/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 4604
end: 4701
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 6368
end: 6387
orientation: reverseComplement

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 5220
end: 5228
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 4710
end: 4766
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 6039
end: 6170
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 5238
end: 5594
orientation: inline

M86(1-17)
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-17)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 3306
end: 3356
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 90
end: 1283
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 1486
end: 1507
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation8/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 2727
end: 3302
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation29
display id: annotation29
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation29/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation29/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 1508
end: 2386
orientation: reverseComplement

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 3363
end: 3392
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 3859
end: 3915
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 4786
end: 5069
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 1363
end: 1382
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 2706
end: 2720
orientation: inline

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 5595
end: 5624
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 3393
end: 3749
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378/1
start: 5150
end: 5234
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
type: ComponentDefinition
persistent identity: IBMc187
display id: IBMc187
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-T-cat-PBAD(SapI-)-RiboJ-BCD22-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-T-cat-PBAD(SapI-)-RiboJ-BCD22-ECF20(102-193)-T Expression plasmid for ECF20 spit at 101/102
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Suffix
sequence annotation: ECF20 102-193
sequence annotation: araC
sequence annotation: L3S2P55
sequence annotation: ECK120033736
sequence annotation: PBAD(SapI-)
sequence annotation: RBS for CDS 1
sequence annotation: VF2
sequence annotation: BBa_B0033
sequence annotation: VR
sequence annotation: cat (BsmBI-)
sequence annotation: cat promoter
sequence annotation: ECK120029600
sequence annotation: ECF20 1-101
sequence annotation: BCD22
sequence annotation: ECK120033737
sequence annotation: tetR promoter
sequence annotation: RFC10 Prefix (full)
sequence annotation: p15A origin of replication
sequence annotation: tetR
sequence annotation: RBS for 2nd CDS
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187_seq/1

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 4862
end: 4882
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 4522
end: 4797
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 1516
end: 2394
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 3994
end: 4050
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 4809
end: 4861
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 2422
end: 2705
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 4453
end: 4460
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 1371
end: 1390
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 2714
end: 2724
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 4977
end: 4996
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 3228
end: 3887
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 3888
end: 3985
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 3045
end: 3134
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 2731
end: 3033
orientation: inline

BCD22
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: BCD22
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 4434
end: 4521
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 3143
end: 3199
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 9
end: 97
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 1494
end: 1515
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 4070
end: 4353
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 5443
end: 6267
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 98
end: 1291
orientation: inline

RBS for 2nd CDS
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: RBS for 2nd CDS
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187/1
start: 4504
end: 4512
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
type: ComponentDefinition
persistent identity: IBMc412
display id: IBMc412
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-169)-GLG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-169)-GLG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: pSEVA PS2 Rev
sequence annotation: BBa_B0030
sequence annotation: RFC10 Prefix (full)
sequence annotation: PBAD(SapI-)
sequence annotation: pSEVA PS1 Fwd
sequence annotation: araC
sequence annotation: SrpR
sequence annotation: cat promoter
sequence annotation: M86N
sequence annotation: cat
sequence annotation: lambda t0 terminator
sequence annotation: ECK120029600
sequence annotation: ECK120033737
sequence annotation: cat (BsmBI-)
sequence annotation: trfA CDS (replication protein)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412_seq/1

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 2601
end: 2617
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 1276
end: 1290
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 57
end: 77
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 984
end: 1267
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 5958
end: 5975
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 78
end: 956
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 1297
end: 1803
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 2694
end: 2791
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 1813
end: 2112
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 2792
end: 3451
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 2547
end: 2641
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 2124
end: 2213
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 2222
end: 2278
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 2307
end: 2447
orientation: reverseComplement

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412/1
start: 3769
end: 4917
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
type: ComponentDefinition
persistent identity: IBMc450
display id: IBMc450
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(177-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(177-236)-H6-Ter Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BCD1
sequence annotation: L3S2P21
sequence annotation: L3S3P21
sequence annotation: VF2
sequence annotation: VR
sequence annotation: H6
sequence annotation: PPhlF
sequence annotation: RBS for CDS2
sequence annotation: RFC10 Suffix
sequence annotation: tetR promoter
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Prefix (full)
sequence annotation: SYNZIP18
sequence annotation: RBS for CDS 1
sequence annotation: PlacIQ
sequence annotation: RiboJ
sequence annotation: phl2
sequence annotation: PhlF
sequence annotation: tetR
sequence annotation: IOT
sequence annotation: mCherry*(177-236)
sequence annotation: ECK120033736
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450_seq/1

BCD1
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2746
end: 2830
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 1516
end: 1576
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2530
end: 2582
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 1371
end: 1390
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 3349
end: 3368
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 3152
end: 3169
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2602
end: 2667
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2816
end: 2824
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 3234
end: 3254
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation3/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 9
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orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 3815
end: 4639
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 1494
end: 1515
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation18/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2831
end: 2956
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2765
end: 2772
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 1581
end: 1634
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2668
end: 2742
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 1635
end: 1662
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 1663
end: 2265
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 98
end: 1291
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2275
end: 2529
orientation: inline

mCherry*(177-236)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(177-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 2972
end: 3151
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450/1
start: 3181
end: 3233
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
type: ComponentDefinition
persistent identity: IBMc386
display id: IBMc386
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-171)-LNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(172-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-171)-LNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(172-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VR
sequence annotation: ECK120029600
sequence annotation: VF2
sequence annotation: p15A origin of replication
sequence annotation: BBa_B0030
sequence annotation: luxR
sequence annotation: ECK120033736
sequence annotation: BBa_B0032
sequence annotation: cat (BsmBI-)
sequence annotation: Plux2
sequence annotation: RFC10 Prefix (full)
sequence annotation: tetR promoter
sequence annotation: ECK120033737
sequence annotation: H6
sequence annotation: cat promoter
sequence annotation: BBa_B0012
sequence annotation: M86N
sequence annotation: PBAD(SapI-)
sequence annotation: mCherry*
sequence annotation: tetR
sequence annotation: BBa_J23117
sequence annotation: M86C
sequence annotation: RFC10 Suffix
sequence annotation: araC
sequence annotation: L3S2P55
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386_seq/1

VR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 6117
end: 6136
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 3568
end: 3657
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 1371
end: 1390
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 6583
end: 7407
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5369
end: 5383
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 4607
end: 5362
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5949
end: 6001
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5538
end: 5550
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 3751
end: 4410
orientation: reverseComplement

Plux2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5476
end: 5529
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 1494
end: 1515
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 9
end: 97
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 2714
end: 2728
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 3666
end: 3722
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5920
end: 5937
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 4411
end: 4508
orientation: reverseComplement

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5427
end: 5467
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 3257
end: 3556
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 2422
end: 2705
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 2735
end: 3252
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 98
end: 1291
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5392
end: 5426
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5560
end: 5721
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 6002
end: 6022
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 1516
end: 2394
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 5725
end: 5919
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386/1
start: 4517
end: 4573
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
type: ComponentDefinition
persistent identity: IBMc252
display id: IBMc252
version: 1
description: pSEVA471(BsbI+)
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA471(BsbI+) Empty plasmid for generating autofluorescence control strains
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA471 Origin of replication: pSC101 Selection Markers: Spectinomycin
owned by: trevorho
date: 17-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: Rep101
sequence annotation: lambda t0 terminator
sequence annotation: PS2
sequence annotation: pSEVA PS2 Rev
sequence annotation: RFC10 Prefix (full)
sequence annotation: SmR
sequence annotation: pSEVA PS1 Fwd
sequence annotation: rrnB T1 terminator
sequence annotation: RFC10 Suffix
sequence annotation: pSC101 ori
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252_seq/1

Rep101
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #993366
ap einfo revcolor: #993366
label: Rep101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 1242
end: 2192
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 2955
end: 3049
orientation: inline

PS2
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: PS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 3009
end: 3025
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 3009
end: 3025
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 2877
end: 2898
orientation: inline

SmR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: SmR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 186
end: 977
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 2719
end: 2736
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: rrnB T1 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 2729
end: 2815
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 2930
end: 2950
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: pSC101 ori
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252/1
start: 2240
end: 2462
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
type: ComponentDefinition
persistent identity: IBMc337
display id: IBMc337
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-deltaI-SMTR(WT)-S-mCherry*(193-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-deltaI-SMTR(WT)-S-mCherry*(193-236)-H6-Ter Expression plasmid for mCherry split at 192/193 inserted with Mtu RecA ?I-SMTR
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PBAD(SapI-)
sequence annotation: araC
sequence annotation: VR
sequence annotation: Mtu RecA 1-109
sequence annotation: RFC10 Prefix (full)
sequence annotation: VF2
sequence annotation: tetR
sequence annotation: tetR promoter
sequence annotation: MtuRecA 384-441
sequence annotation: mCherry*(1-192)
sequence annotation: ECK120033736
sequence annotation: BBa_B0030
sequence annotation: THRB
sequence annotation: H6
sequence annotation: RFC10 Suffix
sequence annotation: mCherry*(193-236)
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337_seq/1

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 2422
end: 2705
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 1516
end: 2394
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 4936
end: 4955
orientation: reverseComplement

Mtu RecA 1-109
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: Mtu RecA 1-109
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 3314
end: 3640
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 1494
end: 1515
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 1371
end: 1390
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 98
end: 1291
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation9/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 9
end: 97
orientation: inline

MtuRecA 384-441
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: MtuRecA 384-441
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation16/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 4430
end: 4603
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 2735
end: 3310
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 4768
end: 4820
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 2714
end: 2728
orientation: inline

THRB
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: THRB
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 3647
end: 4423
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 4739
end: 4756
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 4821
end: 4841
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 4607
end: 4738
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337/1
start: 5402
end: 6226
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
type: ComponentDefinition
persistent identity: IBMc390
display id: IBMc390
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-185)-YNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(186-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-185)-YNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(186-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR
sequence annotation: p15A origin of replication
sequence annotation: BBa_B0030
sequence annotation: L3S2P55
sequence annotation: M86C
sequence annotation: cat promoter
sequence annotation: BBa_B0032
sequence annotation: tetR promoter
sequence annotation: VR
sequence annotation: mCherry*
sequence annotation: Plux2
sequence annotation: cat (BsmBI-)
sequence annotation: PBAD(SapI-)
sequence annotation: BBa_B0012
sequence annotation: araC
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECK120033737
sequence annotation: H6
sequence annotation: ECK120029600
sequence annotation: ECK120033736
sequence annotation: BBa_J23117
sequence annotation: RFC10 Suffix
sequence annotation: M86N
sequence annotation: luxR
sequence annotation: VF2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390_seq/1

tetR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 98
end: 1291
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 6583
end: 7407
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5411
end: 5425
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 4559
end: 4615
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5602
end: 5763
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 4453
end: 4550
orientation: reverseComplement

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5580
end: 5592
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 9
end: 97
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 6117
end: 6136
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 2735
end: 3294
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5518
end: 5571
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 3793
end: 4452
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 2422
end: 2705
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5469
end: 5509
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 1516
end: 2394
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 1494
end: 1515
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 3708
end: 3764
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5920
end: 5937
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 3610
end: 3699
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BBa_B0030
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation14/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2714
end: 2728
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation25/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5949
end: 6001
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5434
end: 5468
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 5767
end: 5919
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 6002
end: 6022
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 3299
end: 3598
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 4649
end: 5404
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390/1
start: 1371
end: 1390
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
type: ComponentDefinition
persistent identity: IBMc395
display id: IBMc395
version: 1
description: pSB3K3-TT-PBAD(SapI-)-B30-His-FKBP-14aa-T7RNAP(181+)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3K3-TT-PBAD(SapI-)-B30-His-FKBP-14aa-T7RNAP(181+)-T Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to FKBP
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3K3 Origin of replication: p15A Selection Markers: Kanamycin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VR
sequence annotation: L3S2P11
sequence annotation: PBAD(SapI-)
sequence annotation: araC
sequence annotation: H6
sequence annotation: kanR
sequence annotation: T7 181+
sequence annotation: linker
sequence annotation: FKBP E coli optimized
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence annotation: L3S3P21
sequence annotation: VF2
sequence annotation: BBa_B0015
sequence annotation: p15A origin of replication
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395_seq/1

VR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 47
end: 66
orientation: reverseComplement

L3S2P11
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: L3S2P11
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 2702
end: 2758
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 3673
end: 3956
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 2767
end: 3645
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 3989
end: 4006
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: kanR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 129
end: 944
orientation: reverseComplement

T7 181+
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: T7 181+
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 4364
end: 6478
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 4346
end: 4363
orientation: inline

FKBP E coli optimized
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: FKBP E coli optimized
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 4007
end: 4327
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 6616
end: 6636
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 2619
end: 2640
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 2641
end: 2693
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 2501
end: 2520
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0015
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 6487
end: 6615
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 1276
end: 2100
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 3965
end: 3979
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395/1
start: 4328
end: 4345
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
type: ComponentDefinition
persistent identity: IBMc483
display id: IBMc483
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-192)-5aa-SYNZIP17-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-192)-5aa-SYNZIP17-Ter Expression plasmid for mCherry N-lobe-SYNZIP17. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PBAD(SapI-)
sequence annotation: VF2
sequence annotation: ampR
sequence annotation: mCherry*(1-192)
sequence annotation: BBa_B0030
sequence annotation: araC
sequence annotation: VR
sequence annotation: L3S2P55
sequence annotation: rep (pMB1)
sequence annotation: SYNZIP17
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483_seq/1

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 2024
end: 2307
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 982
end: 1001
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 1
end: 861
orientation: reverseComplement

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 2337
end: 2912
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 2316
end: 2330
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 1118
end: 1996
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 3198
end: 3217
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 3078
end: 3134
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 3304
end: 3918
orientation: reverseComplement

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483/1
start: 2928
end: 3053
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
type: ComponentDefinition
persistent identity: IBMc226
display id: IBMc226
version: 1
description: pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter Expression plasmid for TetR
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3A5* Origin of replication: p15A Selection Markers: Ampicillin
owned by: trevorho
date: 09-OCT-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PBAD(SapI-)
sequence annotation: tet1
sequence annotation: TetR
sequence annotation: ECK120033736
sequence annotation: VF2
sequence annotation: ampR (BsaI-)
sequence annotation: araC
sequence annotation: VR
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226_seq/1

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 1994
end: 2277
orientation: inline

tet1
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: tet1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 2286
end: 2313
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 2314
end: 2934
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 2946
end: 2998
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 943
end: 962
orientation: inline

ampR (BsaI-)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (BsaI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 3
end: 863
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 1088
end: 1966
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 3114
end: 3133
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 1066
end: 1087
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 2999
end: 3019
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226/1
start: 3580
end: 4404
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
type: ComponentDefinition
persistent identity: IBMc425
display id: IBMc425
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(170-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(170-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: M86C
sequence annotation: PhlF
sequence annotation: L3S2P21
sequence annotation: VF2
sequence annotation: cat promoter
sequence annotation: VR
sequence annotation: PlacIQ
sequence annotation: RFC10 Prefix (full)
sequence annotation: tetR
sequence annotation: SrpR
sequence annotation: ECK120033736
sequence annotation: IOT
sequence annotation: phl2
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Suffix
sequence annotation: cat (BsmBI-)
sequence annotation: L3S3P21
sequence annotation: PPhlF
sequence annotation: tetR promoter
sequence annotation: BBa_B0032
sequence annotation: RiboJ
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425_seq/1

M86C
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 3391
end: 3552
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 2281
end: 2883
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 2134
end: 2194
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 1371
end: 1390
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 2036
end: 2133
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 3867
end: 3886
orientation: reverseComplement

PlacIQ
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 2199
end: 2252
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 1494
end: 1494
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 98
end: 1291
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation11/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3556
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orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation3/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3699
end: 3751
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation10/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2893
end: 3147
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 2253
end: 2280
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 4333
end: 5157
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 3752
end: 3772
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 1517
end: 2035
orientation: reverseComplement

L3S3P21
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 3148
end: 3200
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 3220
end: 3285
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 9
end: 97
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 3369
end: 3381
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
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role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425/1
start: 3286
end: 3360
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
type: ComponentDefinition
persistent identity: IBMc090
display id: IBMc090
version: 1
description: pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2 Plasmid carrying engineered transposon for IBM
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pTHSSd_1 Origin of replication: pMB1 Selection Markers: Kanamycin / Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: Plac
sequence annotation: CmR
sequence annotation: KanR
sequence annotation: BbsI, SapI modified transpsoson
sequence annotation: R2
sequence annotation: modified R1 for SapI
sequence annotation: CG>AA (SapI)
sequence annotation: ori
sequence annotation: ECK120033737
sequence annotation: lambda t0 terminator
sequence annotation: overhang for BbsI
sequence annotation: overhang by SapI
sequence annotation: Reporter lacZα
sequence annotation: G>T (BbsI)
sequence annotation: GCA>TTC (BbsI)
sequence annotation: ECK120029600
sequence annotation: C>A (SapI)
sequence annotation: A>G (Glycine)
sequence annotation: modified R1 for BbsI
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090_seq/1

Plac
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: Plac
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2472
end: 2501
orientation: reverseComplement

CmR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: CmR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2826
end: 3485
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: KanR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 1143
end: 1958
orientation: inline

BbsI, SapI modified transpsoson
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: BbsI, SapI modified transpsoson
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 966
end: 2632
orientation: inline

R2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: R2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000286
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2584
end: 2605
orientation: reverseComplement

modified R1 for SapI
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: modified R1 for SapI
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000286
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2606
end: 2627
orientation: reverseComplement

CG>AA (SapI)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: CG>AA (SapI)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2626
end: 2627
orientation: reverseComplement

ori
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: ori
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 183
end: 771
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2072
end: 2128
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 3506
end: 3600
orientation: inline

overhang for BbsI
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: overhang for BbsI
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 966
end: 969
orientation: inline

overhang by SapI
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: overhang by SapI
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2630
end: 2632
orientation: inline

Reporter lacZα
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: Reporter lacZα
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2131
end: 2427
orientation: reverseComplement

G>T (BbsI)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: G>T (BbsI)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 973
end: 973
orientation: inline

GCA>TTC (BbsI)
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: GCA>TTC (BbsI)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 975
end: 977
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 1974
end: 2063
orientation: inline

R2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: R2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000286
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 993
end: 1014
orientation: inline

C>A (SapI)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: C>A (SapI)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 2624
end: 2624
orientation: reverseComplement

A>G (Glycine)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: A>G (Glycine)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 968
end: 968
orientation: inline

modified R1 for BbsI
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: modified R1 for BbsI
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
role: SO:0000286
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090/1
start: 971
end: 992
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
type: ComponentDefinition
persistent identity: IBMc429
display id: IBMc429
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(177-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(177-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: SrpR
sequence annotation: ECK120033736
sequence annotation: PhlF
sequence annotation: tetR promoter
sequence annotation: BBa_B0032
sequence annotation: PPhlF
sequence annotation: tetR
sequence annotation: IOT
sequence annotation: PlacIQ
sequence annotation: cat promoter
sequence annotation: cat (BsmBI-)
sequence annotation: L3S3P21
sequence annotation: M86C
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Suffix
sequence annotation: L3S2P21
sequence annotation: VF2
sequence annotation: phl2
sequence annotation: RFC10 Prefix (full)
sequence annotation: VR
sequence annotation: RiboJ
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429_seq/1

SrpR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3556
end: 3666
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3678
end: 3730
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 2281
end: 2883
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 9
end: 97
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3369
end: 3381
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3220
end: 3285
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 98
end: 1291
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 2893
end: 3147
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 2199
end: 2252
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 2036
end: 2133
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 1517
end: 2035
orientation: reverseComplement

L3S3P21
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3148
end: 3200
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3391
end: 3552
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 4312
end: 5136
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3731
end: 3751
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 2134
end: 2194
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 1371
end: 1390
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 2253
end: 2280
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 1494
end: 1494
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3846
end: 3865
orientation: reverseComplement

RiboJ
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429/1
start: 3286
end: 3360
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
type: ComponentDefinition
persistent identity: IBMc334
display id: IBMc334
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter Expression plasmid for mCherry split at 192/193 inserted with Npu DnaE, caged intein fused with FKBP/FRB
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: (NpuC1-30(K2E,R6E,K11E))x2
sequence annotation: NpuN(51-102)
sequence annotation: tetR
sequence annotation: VR
sequence annotation: ECK120029600
sequence annotation: RFC10 Suffix
sequence annotation: cat (BsmBI-)
sequence annotation: mCherry*(193-236)
sequence annotation: VF2
sequence annotation: L3S2P55
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECK120033736
sequence annotation: BBa_B0030
sequence annotation: NpuN(E52K,E54K,D85K,E89R,E91K)
sequence annotation: p15A origin of replication
sequence annotation: Caspase3 cut site
sequence annotation: RiboJ
sequence annotation: BCD1
sequence annotation: araC
sequence annotation: cat promoter
sequence annotation: RBS for CDS2
sequence annotation: PBAD(SapI-)
sequence annotation: FRB E coli optimized
sequence annotation: ECK120033737
sequence annotation: mCherry*(1-192)
sequence annotation: RBS for CDS 1
sequence annotation: FKBP E coli optimized
sequence annotation: tetR promoter
sequence annotation: NpuC
sequence annotation: H6
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334_seq/1

(NpuC1-30(K2E,R6E,K11E))x2
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: (NpuC1-30(K2E,R6E,K11E))x2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 3680
end: 3862
orientation: inline

NpuN(51-102)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: NpuN(51-102)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 5987
end: 6142
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
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type: Range
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start: 98
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VR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
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label: VR
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation19/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 6631
end: 6650
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
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label: ECK120029600
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation3/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 4216
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orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #9eafd2
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label: RFC10 Suffix
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation21/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 6516
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cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation29
display id: annotation29
version: 1
ap einfo fwdcolor: #ff9ccd
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label: cat (BsmBI-)
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation29/range/1

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type: Range
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version: 1
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start: 4399
end: 5058
orientation: reverseComplement

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation30
display id: annotation30
version: 1
ap einfo fwdcolor: #faac61
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label: mCherry*(193-236)
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation30/range/1

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type: Range
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version: 1
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start: 6302
end: 6433
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #75c6a9
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label: VF2
owned by: trevorho
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation22/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 1371
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orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation23/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 5165
end: 5221
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 1494
end: 1515
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation25/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 6463
end: 6515
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 2714
end: 2728
orientation: inline

NpuN(E52K,E54K,D85K,E89R,E91K)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: NpuN(E52K,E54K,D85K,E89R,E91K)
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation11/range/1
type: Range
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display id: range
version: 1
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start: 3323
end: 3628
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 7097
end: 7921
orientation: inline

Caspase3 cut site
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: Caspase3 cut site
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 3644
end: 3655
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 5530
end: 5604
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 5605
end: 5689
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 1516
end: 2394
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 5059
end: 5156
orientation: reverseComplement

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 5675
end: 5683
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation31
display id: annotation31
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation31/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation31/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 2422
end: 2705
orientation: inline

FRB E coli optimized
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: FRB E coli optimized
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 5693
end: 5971
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 4314
end: 4370
orientation: reverseComplement

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation28
display id: annotation28
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation28/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation28/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 2735
end: 3309
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 5624
end: 5631
orientation: inline

FKBP E coli optimized
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: FKBP E coli optimized
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 3884
end: 4204
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 9
end: 97
orientation: inline

Caspase3 cut site
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: Caspase3 cut site
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 6158
end: 6169
orientation: inline

NpuC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: NpuC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 6185
end: 6298
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation27
display id: annotation27
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation27/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation27/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 6434
end: 6451
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
start: 5241
end: 5524
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
type: ComponentDefinition
persistent identity: IBMc393
display id: IBMc393
version: 1
description: pSB4A3-T-PT7-B30-mScarlet-I-3T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB4A3-T-PT7-B30-mScarlet-I-3T T7 RNAP polymerase reporter plasmid
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB4A3 Origin of replication: pSC101 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PT7
sequence annotation: BBa_B0030
sequence annotation: mScarlet-I
sequence annotation: PTH TERM terminator
sequence annotation: BBa_B1006
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECK120029600
sequence annotation: VF2
sequence annotation: T7Tphi terminator
sequence annotation: RFC10 Suffix
sequence annotation: VR
sequence annotation: repA
sequence annotation: pSC101 origin
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393_seq/1

PT7
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: PT7
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 2593
end: 2613
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 2622
end: 2636
orientation: inline

mScarlet-I
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: mScarlet-I
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 2643
end: 3338
orientation: inline

PTH TERM terminator
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: PTH TERM terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 3534
end: 3564
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: BBa_B1006
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 3350
end: 3388
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 2474
end: 2495
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 2496
end: 2585
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 2356
end: 2375
orientation: inline

T7Tphi terminator
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: T7Tphi terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 3397
end: 3525
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 3565
end: 3585
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 3719
end: 3738
orientation: reverseComplement

repA
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: repA
multi range type: join
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation5/range1/1
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation5/range0/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation5/range1/1
type: Range
persistent identity: range1
display id: range1
version: 1
position: position0
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 1
end: 490
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation5/range0/1
type: Range
persistent identity: range0
display id: range0
version: 1
position: position1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 3946
end: 4406
orientation: reverseComplement

pSC101 origin
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: pSC101 origin
multi range type: join
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation4/range0/1
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation4/range1/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation4/range0/1
type: Range
persistent identity: range0
display id: range0
version: 1
position: position1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 3745
end: 4406
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/annotation4/range1/1
type: Range
persistent identity: range1
display id: range1
version: 1
position: position0
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393/1
start: 1
end: 1375
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
type: ComponentDefinition
persistent identity: IBMc402
display id: IBMc402
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-QLG-M86N-cat-Plux2-B32-M86C-S-mCherry*(193-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-QLG-M86N-cat-Plux2-B32-M86C-S-mCherry*(193-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: L3S2P55
sequence annotation: tetR
sequence annotation: PBAD(SapI-)
sequence annotation: RFC10 Suffix
sequence annotation: M86C
sequence annotation: ECK120033737
sequence annotation: BBa_B0032
sequence annotation: H6
sequence annotation: VR
sequence annotation: tetR promoter
sequence annotation: p15A origin of replication
sequence annotation: BBa_B0012
sequence annotation: VF2
sequence annotation: BBa_B0030
sequence annotation: mCherry*
sequence annotation: Plux2
sequence annotation: cat promoter
sequence annotation: M86N
sequence annotation: ECK120029600
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECK120033736
sequence annotation: luxR
sequence annotation: cat (BsmBI-)
sequence annotation: araC
sequence annotation: BBa_J23117
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402_seq/1

L3S2P55
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 4580
end: 4636
orientation: reverseComplement

tetR
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 98
end: 1291
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 2422
end: 2705
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 6002
end: 6022
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5623
end: 5784
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 3729
end: 3785
orientation: reverseComplement

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5601
end: 5613
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5920
end: 5937
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 6117
end: 6136
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 9
end: 97
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 6583
end: 7407
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5490
end: 5530
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 1371
end: 1390
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5432
end: 5446
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5788
end: 5919
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5539
end: 5592
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 2738
end: 3310
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 4474
end: 4571
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 2714
end: 2728
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 3320
end: 3619
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 3631
end: 3720
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 1494
end: 1515
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5949
end: 6001
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 4670
end: 5425
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 3814
end: 4473
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 1516
end: 2394
orientation: reverseComplement

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402/1
start: 5455
end: 5489
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
type: ComponentDefinition
persistent identity: IBMc052
display id: IBMc052
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-T mCherry expression plasmid. Activity reference in IBM experimenton mCherry
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 17-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0030
sequence annotation: tetR
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: VF2
sequence annotation: VR
sequence annotation: ECK120033736
sequence annotation: mCherry*
sequence annotation: tetR promoter
sequence annotation: araC
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052_seq/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 2714
end: 2728
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 98
end: 1291
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 4088
end: 4912
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 1494
end: 1515
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 3507
end: 3527
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 1371
end: 1390
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 3622
end: 3641
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 3454
end: 3506
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 2735
end: 3442
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 9
end: 97
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052/1
start: 1516
end: 2394
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
type: ComponentDefinition
persistent identity: IBMc407
display id: IBMc407
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-77)-LGG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-77)-LGG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat (BsmBI-)
sequence annotation: M86N
sequence annotation: araC
sequence annotation: ECK120029600
sequence annotation: PBAD(SapI-)
sequence annotation: pSEVA PS2 Rev
sequence annotation: BBa_B0030
sequence annotation: RFC10 Prefix (full)
sequence annotation: trfA CDS (replication protein)
sequence annotation: SrpR
sequence annotation: lambda t0 terminator
sequence annotation: cat
sequence annotation: cat promoter
sequence annotation: pSEVA PS1 Fwd
sequence annotation: ECK120033737
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407_seq/1

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 2031
end: 2171
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 1537
end: 1836
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 78
end: 956
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 1848
end: 1937
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 984
end: 1267
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 2325
end: 2341
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 1276
end: 1290
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 57
end: 77
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 3493
end: 4641
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 1297
end: 1527
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 2271
end: 2365
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 2516
end: 3175
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 2418
end: 2515
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 5682
end: 5699
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407/1
start: 1946
end: 2002
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
type: ComponentDefinition
persistent identity: IBMc401
display id: IBMc401
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-191)-VHG-M86N-cat-Plux2-B32-M86C-S-mCherry*(192-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-191)-VHG-M86N-cat-Plux2-B32-M86C-S-mCherry*(192-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: p15A origin of replication
sequence annotation: BBa_B0030
sequence annotation: mCherry*
sequence annotation: BBa_B0012
sequence annotation: cat (BsmBI-)
sequence annotation: Plux2
sequence annotation: tetR
sequence annotation: araC
sequence annotation: ECK120033736
sequence annotation: BBa_J23117
sequence annotation: VF2
sequence annotation: cat promoter
sequence annotation: L3S2P55
sequence annotation: luxR
sequence annotation: BBa_B0032
sequence annotation: M86C
sequence annotation: tetR promoter
sequence annotation: VR
sequence annotation: M86N
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: ECK120029600
sequence annotation: H6
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033737
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401_seq/1

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 6583
end: 7407
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 2714
end: 2728
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5785
end: 5919
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5487
end: 5527
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 3811
end: 4470
orientation: reverseComplement

Plux2
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5536
end: 5589
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 98
end: 1291
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 1516
end: 2394
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5949
end: 6001
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5452
end: 5486
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 1371
end: 1390
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 4471
end: 4568
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 4577
end: 4633
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 4667
end: 5422
orientation: reverseComplement

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5598
end: 5610
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5620
end: 5781
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 9
end: 97
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 6117
end: 6136
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 3317
end: 3616
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 1494
end: 1515
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 2735
end: 3312
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5429
end: 5443
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 6002
end: 6022
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 3628
end: 3717
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 5920
end: 5937
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 2422
end: 2705
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401/1
start: 3726
end: 3782
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
type: ComponentDefinition
persistent identity: IBMc457
display id: IBMc457
version: 1
description: pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-166)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(167-207)-HA-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-166)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(167-207)-HA-Ter Expression plasmid for tagged TetR split at 166/167 with SYNZIP17 & SYNZIP18
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3A5* Origin of replication: p15A Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ECK120033736
sequence annotation: ampR (BsaI-)
sequence annotation: BCD1
sequence annotation: p15A origin of replication
sequence annotation: araC
sequence annotation: RFC10 Suffix
sequence annotation: TetR(167-207)
sequence annotation: BBa_B0030
sequence annotation: PBAD(SapI-)
sequence annotation: RFC10 Prefix (full)
sequence annotation: RBS for CDS2
sequence annotation: TetR(2-166)
sequence annotation: HA tag
sequence annotation: SYNZIP17
sequence annotation: VR
sequence annotation: VF2
sequence annotation: SYNZIP18
sequence annotation: H6
sequence annotation: RBS for CDS 1
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457_seq/1

ECK120033736
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3354
end: 3406
orientation: inline

ampR (BsaI-)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (BsaI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3
end: 863
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 2967
end: 3051
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3988
end: 4812
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 1088
end: 1966
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3407
end: 3427
orientation: inline

TetR(167-207)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR(167-207)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3193
end: 3315
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 2286
end: 2300
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 1994
end: 2277
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 1066
end: 1087
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3037
end: 3045
orientation: inline

TetR(2-166)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR(2-166)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 2328
end: 2822
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: HA tag
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3316
end: 3342
orientation: inline

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 2838
end: 2963
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3522
end: 3541
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 943
end: 962
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 3052
end: 3177
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 2310
end: 2327
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457/1
start: 2986
end: 2993
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
type: ComponentDefinition
persistent identity: IBMc449
display id: IBMc449
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(174-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(174-236)-H6-Ter Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR promoter
sequence annotation: p15A origin of replication
sequence annotation: L3S2P21
sequence annotation: PlacIQ
sequence annotation: RBS for CDS2
sequence annotation: VR
sequence annotation: mCherry*(174-236)
sequence annotation: L3S3P21
sequence annotation: RFC10 Suffix
sequence annotation: phl2
sequence annotation: BCD1
sequence annotation: RBS for CDS 1
sequence annotation: VF2
sequence annotation: RFC10 Prefix (full)
sequence annotation: IOT
sequence annotation: SYNZIP18
sequence annotation: H6
sequence annotation: tetR
sequence annotation: ECK120033736
sequence annotation: PhlF
sequence annotation: PPhlF
sequence annotation: RiboJ
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449_seq/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 9
end: 97
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 3824
end: 4648
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 1516
end: 1576
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 1581
end: 1634
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2816
end: 2824
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 3358
end: 3377
orientation: reverseComplement

mCherry*(174-236)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(174-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2972
end: 3160
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2530
end: 2582
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 3243
end: 3263
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 1635
end: 1662
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2746
end: 2830
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2765
end: 2772
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 1371
end: 1390
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 1494
end: 1515
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2275
end: 2529
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2831
end: 2956
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 3161
end: 3178
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 98
end: 1291
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 3190
end: 3242
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 1663
end: 2265
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2602
end: 2667
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449/1
start: 2668
end: 2742
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
type: ComponentDefinition
persistent identity: IBMc476
display id: IBMc476
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-159)-5aa-SYNZIP17-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-159)-5aa-SYNZIP17-Ter Expression plasmid for mCherry N-lobe-SYNZIP17. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0030
sequence annotation: ampR
sequence annotation: VF2
sequence annotation: PBAD(SapI-)
sequence annotation: rep (pMB1)
sequence annotation: araC
sequence annotation: mCherry*(1-159)
sequence annotation: L3S2P55
sequence annotation: VR
sequence annotation: SYNZIP17
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476_seq/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 2316
end: 2330
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 1
end: 861
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 982
end: 1001
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 2024
end: 2307
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 3205
end: 3819
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 1118
end: 1996
orientation: reverseComplement

mCherry*(1-159)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-159)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 2337
end: 2813
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 2979
end: 3035
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 3099
end: 3118
orientation: reverseComplement

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476/1
start: 2829
end: 2954
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
type: ComponentDefinition
persistent identity: IBMc383
display id: IBMc383
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-156)-PDG-M86N-cat-Plux2-B32-M86C-S-mCherry*(157-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-156)-PDG-M86N-cat-Plux2-B32-M86C-S-mCherry*(157-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ECK120029600
sequence annotation: araC
sequence annotation: p15A origin of replication
sequence annotation: mCherry*
sequence annotation: luxR
sequence annotation: VR
sequence annotation: cat (BsmBI-)
sequence annotation: ECK120033737
sequence annotation: M86C
sequence annotation: tetR
sequence annotation: M86N
sequence annotation: BBa_B0012
sequence annotation: RFC10 Prefix (full)
sequence annotation: BBa_B0030
sequence annotation: BBa_B0032
sequence annotation: Plux2
sequence annotation: PBAD(SapI-)
sequence annotation: tetR promoter
sequence annotation: H6
sequence annotation: VF2
sequence annotation: L3S2P55
sequence annotation: cat promoter
sequence annotation: RFC10 Suffix
sequence annotation: ECK120033736
sequence annotation: BBa_J23117
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383_seq/1

ECK120029600
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 3523
end: 3612
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 1516
end: 2394
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 6583
end: 7407
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 2735
end: 3207
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 4562
end: 5317
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5680
end: 5919
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 6117
end: 6136
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 3706
end: 4365
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 3621
end: 3677
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5515
end: 5676
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 98
end: 1291
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 3212
end: 3511
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5382
end: 5422
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 1494
end: 1515
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 2714
end: 2728
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5493
end: 5505
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5431
end: 5484
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 2422
end: 2705
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 9
end: 97
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5920
end: 5937
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 1371
end: 1390
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 4472
end: 4528
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 4366
end: 4463
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 6002
end: 6022
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5949
end: 6001
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5347
end: 5381
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383/1
start: 5324
end: 5338
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
type: ComponentDefinition
persistent identity: IBMc460
display id: IBMc460
version: 1
description: pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI Plasmid carrying Golden Gate substitution insert for IBM, gp41-1 intien, using PPhlF as C-lobe promoter
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicillin / Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PhlF
sequence annotation: IOT
sequence annotation: gp41-1N
sequence annotation: ampR (rev)
sequence annotation: ECK120029600
sequence annotation: RiboJ
sequence annotation: RFC10 Suffix
sequence annotation: gp41-1C
sequence annotation: PlacIQ
sequence annotation: VF2
sequence annotation: BBa_B0032
sequence annotation: rep (pMB1)
sequence annotation: VR
sequence annotation: ECK120033737
sequence annotation: L3S2P21
sequence annotation: RFC10 Prefix (full)
sequence annotation: L3S3P21
sequence annotation: phl2
sequence annotation: PPhlF
sequence annotation: cat (BsmBI-)
sequence annotation: cat promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460_seq/1

PhlF
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 2500
end: 3102
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3112
end: 3366
orientation: inline

gp41-1N
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: gp41-1N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 1137
end: 1400
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (rev)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 1
end: 861
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 1412
end: 1501
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3505
end: 3579
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3732
end: 3752
orientation: inline

gp41-1C
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: gp41-1C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3607
end: 3717
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 2418
end: 2471
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 982
end: 1001
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3588
end: 3600
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3916
end: 4530
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3810
end: 3829
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 1510
end: 1566
orientation: reverseComplement

L3S2P21
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 2353
end: 2413
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 1100
end: 1121
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3367
end: 3419
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 2472
end: 2499
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 3439
end: 3504
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 1595
end: 2254
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460/1
start: 2255
end: 2352
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
type: ComponentDefinition
persistent identity: IBMc065
display id: IBMc065
version: 1
description: pSB3T5(BsaI-)-PBAD-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-T Outcome simulation control for IBM on mCherry
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 17-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: p15A origin of replication
sequence annotation: L3S2P55
sequence annotation: RFC10 Suffix
sequence annotation: BBa_J23117
sequence annotation: ECK120033736
sequence annotation: cat promoter
sequence annotation: VF2
sequence annotation: luxR
sequence annotation: mCherry*
sequence annotation: ECK120029600
sequence annotation: araC
sequence annotation: M86C
sequence annotation: VR
sequence annotation: M86N
sequence annotation: BBa_B0030
sequence annotation: BBa_B0032
sequence annotation: Plux2
sequence annotation: BBa_B0012
sequence annotation: tetR
sequence annotation: tetR promoter
sequence annotation: cat (BsmBI-)
sequence annotation: ECK120033737
sequence annotation: RFC10 Prefix (full)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065_seq/1

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 6565
end: 7389
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 4481
end: 4537
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 5984
end: 6004
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 5356
end: 5390
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 5931
end: 5983
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation1/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 4375
end: 4472
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 1371
end: 1390
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation21/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 4571
end: 5326
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 5689
end: 5919
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 2735
end: 3211
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 3532
end: 3621
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 1516
end: 2394
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 5524
end: 5685
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 6099
end: 6118
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 3221
end: 3520
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 2714
end: 2728
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 5502
end: 5514
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 5440
end: 5493
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 5391
end: 5431
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 98
end: 1291
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 9
end: 97
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 3715
end: 4374
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 3630
end: 3686
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 1494
end: 1515
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065/1
start: 5333
end: 5347
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
type: ComponentDefinition
persistent identity: IBMc414
display id: IBMc414
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-171)-RSG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-171)-RSG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ECK120029600
sequence annotation: pSEVA PS2 Rev
sequence annotation: BBa_B0030
sequence annotation: SrpR
sequence annotation: cat
sequence annotation: pSEVA PS1 Fwd
sequence annotation: cat (BsmBI-)
sequence annotation: ECK120033737
sequence annotation: cat promoter
sequence annotation: araC
sequence annotation: PBAD(SapI-)
sequence annotation: M86N
sequence annotation: trfA CDS (replication protein)
sequence annotation: RFC10 Prefix (full)
sequence annotation: lambda t0 terminator
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414_seq/1

ECK120029600
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 2130
end: 2219
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 2607
end: 2623
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 1276
end: 1290
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 1297
end: 1809
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 2798
end: 3457
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 5964
end: 5981
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 2313
end: 2453
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 2228
end: 2284
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 2700
end: 2797
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 78
end: 956
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 984
end: 1267
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 1819
end: 2118
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 3775
end: 4923
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 57
end: 77
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414/1
start: 2553
end: 2647
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
type: ComponentDefinition
persistent identity: IBMc249
display id: IBMc249
version: 1
description: pSB3A5*(BsaI-)(empty)
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3A5*(BsaI-)(empty) Empty plasmid for generating autofluorescence control strains
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3A5* Origin of replication: p15A Selection Markers: Ampicillin
owned by: trevorho
date: 17-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VF2
sequence annotation: ampR (BsaI-)
sequence annotation: VR
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249_seq/1

VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
start: 1114
end: 1133
orientation: inline

ampR (BsaI-)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (BsaI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
start: 174
end: 1034
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
start: 1366
end: 1385
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249/1
start: 1832
end: 2656
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
type: ComponentDefinition
persistent identity: IBMc454
display id: IBMc454
version: 1
description: pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR-HA-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR-HA-Ter Expression plasmid for tagged TetR
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3A5* Origin of replication: p15A Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Prefix (full)
sequence annotation: BBa_B0030
sequence annotation: HA tag
sequence annotation: VR
sequence annotation: ampR (BsaI-)
sequence annotation: ECK120033736
sequence annotation: VF2
sequence annotation: PBAD(SapI-)
sequence annotation: araC
sequence annotation: RFC10 Suffix
sequence annotation: H6
sequence annotation: p15A origin of replication
sequence annotation: TetR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454_seq/1

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 1066
end: 1087
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 2286
end: 2300
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: HA tag
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 2946
end: 2972
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 3152
end: 3171
orientation: reverseComplement

ampR (BsaI-)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (BsaI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 3
end: 863
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 2984
end: 3036
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 943
end: 962
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 1994
end: 2277
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 1088
end: 1966
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 3037
end: 3057
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 2310
end: 2327
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 3618
end: 4442
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454/1
start: 2328
end: 2945
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
type: ComponentDefinition
persistent identity: IBMc227
display id: IBMc227
version: 1
description: pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter Expression vector to generate ORF insertion library in IBM on TetR
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3A5* Origin of replication: p15A Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: araC
sequence annotation: PBAD(SapI-)
sequence annotation: spacing sequence
sequence annotation: TetR(1-20)
sequence annotation: TetR(188-207)
sequence annotation: VF2
sequence annotation: ECK120033736
sequence annotation: RFC10 Prefix (full)
sequence annotation: ampR (BsaI-)
sequence annotation: RFC10 Suffix
sequence annotation: p15A origin of replication
sequence annotation: tet1
sequence annotation: VR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227_seq/1

araC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 1259
end: 2137
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 2165
end: 2448
orientation: inline

spacing sequence
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: spacing sequence
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 2552
end: 2567
orientation: inline

TetR(1-20)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR(1-20)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 2485
end: 2544
orientation: inline

TetR(188-207)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR(188-207)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 2575
end: 2634
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 1114
end: 1133
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 2646
end: 2698
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 1237
end: 1258
orientation: inline

ampR (BsaI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (BsaI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 174
end: 1034
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 2699
end: 2719
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 3280
end: 4104
orientation: inline

tet1
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: tet1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 2457
end: 2484
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227/1
start: 2814
end: 2833
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
type: ComponentDefinition
persistent identity: IBMc371
display id: IBMc371
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-85)-ELG-ER-LBD-S-M86(86-154)-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-85)-ELG-ER-LBD-S-M86(86-154)-S-ECF20(102-193)-T Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 85/86 by ER-LBD
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: M86(85-154)
sequence annotation: tetR promoter
sequence annotation: VR
sequence annotation: M86(1-76)
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033736
sequence annotation: RFC10 Suffix
sequence annotation: ECF20 1-101
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECF20 102-193
sequence annotation: VF2
sequence annotation: BBa_B0033
sequence annotation: p15A origin of replication
sequence annotation: tetR
sequence annotation: ER-LBD
sequence annotation: araC
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371_seq/1

M86(85-154)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(85-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 4048
end: 4254
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 9
end: 97
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 4713
end: 4732
orientation: reverseComplement

M86(1-76)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-76)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 3037
end: 3291
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 2422
end: 2705
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 4545
end: 4597
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 4598
end: 4618
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 2731
end: 3033
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 1494
end: 1515
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 4258
end: 4533
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 1371
end: 1390
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 2714
end: 2724
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 5179
end: 6003
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 98
end: 1291
orientation: inline

ER-LBD
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ER-LBD
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 3301
end: 4044
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371/1
start: 1516
end: 2394
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
type: ComponentDefinition
persistent identity: IBMc330
display id: IBMc330
version: 1
description: pSEVA171-PSrpR-B30-mScarlet-I-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA171-PSrpR-B30-mScarlet-I-Ter SrpR reporter plasmid
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA171 Origin of replication: pSC101 Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: Rep101
sequence annotation: pSC101 ori
sequence annotation: fd terminator
sequence annotation: AmpR
sequence annotation: lambda t0 terminator
sequence annotation: AmpR promoter
sequence annotation: pSEVA PS2 Rev
sequence annotation: BBa_B0030
sequence annotation: rrnB T1 terminator
sequence annotation: L3S2P11
sequence annotation: 2ndVal-mScarlet-I
sequence annotation: PSrpR
sequence annotation: pSEVA PS1 Fwd
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330_seq/1

Rep101
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #993366
ap einfo revcolor: #993366
label: Rep101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 2644
end: 3594
orientation: reverseComplement

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: pSC101 ori
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation3/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3642
end: 3864
orientation: inline

fd terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: fd terminator
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation1/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2339
end: 2378
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: AmpR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 1475
end: 2335
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation12/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 1208
end: 1302
orientation: inline

AmpR promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: AmpR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 1383
end: 1474
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 1262
end: 1278
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 394
end: 408
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: rrnB T1 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 140
end: 226
orientation: reverseComplement

L3S2P11
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P11
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation6/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 1117
end: 1173
orientation: inline

2ndVal-mScarlet-I
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 2ndVal-mScarlet-I
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 415
end: 1113
orientation: inline

PSrpR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PSrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 319
end: 385
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330/1
start: 130
end: 147
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
type: ComponentDefinition
persistent identity: IBMc387
display id: IBMc387
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-173)-DGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(174-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-173)-DGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(174-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: araC
sequence annotation: VR
sequence annotation: L3S2P55
sequence annotation: BBa_B0030
sequence annotation: M86C
sequence annotation: Plux2
sequence annotation: RFC10 Suffix
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: H6
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033736
sequence annotation: luxR
sequence annotation: p15A origin of replication
sequence annotation: mCherry*
sequence annotation: tetR promoter
sequence annotation: RFC10 Prefix (full)
sequence annotation: cat promoter
sequence annotation: VF2
sequence annotation: ECK120029600
sequence annotation: ECK120033737
sequence annotation: M86N
sequence annotation: tetR
sequence annotation: BBa_J23117
sequence annotation: cat (BsmBI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387_seq/1

araC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 1516
end: 2394
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 6117
end: 6136
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 4523
end: 4579
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5375
end: 5389
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5566
end: 5727
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5482
end: 5535
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 6002
end: 6022
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5544
end: 5556
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5433
end: 5473
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5920
end: 5937
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 2422
end: 2705
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5949
end: 6001
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 4613
end: 5368
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 6583
end: 7407
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 2735
end: 3258
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 9
end: 97
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 1494
end: 1515
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 4417
end: 4514
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation8/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 1371
end: 1390
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 2714
end: 2728
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 3574
end: 3663
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 3672
end: 3728
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 3263
end: 3562
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 98
end: 1291
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5398
end: 5432
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 3757
end: 4416
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387/1
start: 5731
end: 5919
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
type: ComponentDefinition
persistent identity: IBMc380
display id: IBMc380
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry*(193-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry*(193-236)-H6-T Expression plasmid for mCherry split at 192/193 split by M86 intein split at 39/40 by acVHH
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RBS for CDS 1
sequence annotation: RiboJ
sequence annotation: BCD1
sequence annotation: mCherry*(1-192)
sequence annotation: H6
sequence annotation: acVHH
sequence annotation: M86(1-39)
sequence annotation: M86(40-154)
sequence annotation: cat promoter
sequence annotation: ECK120029600
sequence annotation: VF2
sequence annotation: RFC10 Prefix (full)
sequence annotation: VR
sequence annotation: L3S2P55
sequence annotation: PBAD(SapI-)
sequence annotation: araC
sequence annotation: RFC10 Suffix
sequence annotation: 2x(GGGGS) linker
sequence annotation: RBS for CDS2
sequence annotation: ECK120033736
sequence annotation: tetR
sequence annotation: mCherry*(193-236)
sequence annotation: BBa_B0030
sequence annotation: cat (BsmBI-)
sequence annotation: tetR promoter
sequence annotation: p15A origin of replication
sequence annotation: ECK120033737
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380_seq/1

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 5235
end: 5242
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 5141
end: 5215
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 5216
end: 5300
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation29
display id: annotation29
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation29/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation29/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 2727
end: 3302
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 6171
end: 6188
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 3459
end: 3815
orientation: inline

M86(1-39)
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-39)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 3306
end: 3422
orientation: inline

M86(40-154)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(40-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 5691
end: 6035
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation28
display id: annotation28
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation28/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation28/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 4670
end: 4767
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 3827
end: 3916
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 1363
end: 1382
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 1486
end: 1507
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 5304
end: 5660
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 6368
end: 6387
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 4776
end: 4832
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 2414
end: 2697
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 1508
end: 2386
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 6253
end: 6273
orientation: inline

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 5661
end: 5690
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 5286
end: 5294
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 4852
end: 5135
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation27
display id: annotation27
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation27/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation27/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 6200
end: 6252
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 90
end: 1283
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 6039
end: 6170
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 2706
end: 2720
orientation: inline

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 3429
end: 3458
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 4010
end: 4669
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 1
end: 89
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 6834
end: 7658
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380/1
start: 3925
end: 3981
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
type: ComponentDefinition
persistent identity: IBMc481
display id: IBMc481
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-185)-5aa-SYNZIP17-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-185)-5aa-SYNZIP17-Ter Expression plasmid for mCherry N-lobe-SYNZIP17. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ampR
sequence annotation: L3S2P55
sequence annotation: PBAD(SapI-)
sequence annotation: VF2
sequence annotation: araC
sequence annotation: mCherry*(1-185)
sequence annotation: VR
sequence annotation: rep (pMB1)
sequence annotation: SYNZIP17
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481_seq/1

ampR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 1
end: 861
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 3057
end: 3113
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 2024
end: 2307
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 982
end: 1001
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 1118
end: 1996
orientation: reverseComplement

mCherry*(1-185)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-185)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 2340
end: 2891
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 3177
end: 3196
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 3283
end: 3897
orientation: reverseComplement

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 2907
end: 3032
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481/1
start: 2316
end: 2330
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
type: ComponentDefinition
persistent identity: IBMc473
display id: IBMc473
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-185)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-185)-Ter Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0030
sequence annotation: VR
sequence annotation: mCherry*(1-185)
sequence annotation: L3S2P55
sequence annotation: araC
sequence annotation: ampR
sequence annotation: rep (pMB1)
sequence annotation: VF2
sequence annotation: PBAD(SapI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473_seq/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
start: 2316
end: 2330
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
start: 3036
end: 3055
orientation: reverseComplement

mCherry*(1-185)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-185)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
start: 2340
end: 2891
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
start: 2916
end: 2972
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
start: 1118
end: 1996
orientation: reverseComplement

ampR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
start: 1
end: 861
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation8/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3142
end: 3756
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation1/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 982
end: 1001
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/annotation3/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473/1
start: 2024
end: 2307
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
type: ComponentDefinition
persistent identity: IBMc447
display id: IBMc447
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(122-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(122-236)-H6-Ter Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: mCherry*(122-236)
sequence annotation: phl2
sequence annotation: BCD1
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: PPhlF
sequence annotation: RBS for CDS 1
sequence annotation: L3S2P21
sequence annotation: RiboJ
sequence annotation: RBS for CDS2
sequence annotation: SYNZIP18
sequence annotation: p15A origin of replication
sequence annotation: tetR
sequence annotation: PlacIQ
sequence annotation: VR
sequence annotation: L3S3P21
sequence annotation: IOT
sequence annotation: PhlF
sequence annotation: ECK120033736
sequence annotation: H6
sequence annotation: VF2
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447_seq/1

mCherry*(122-236)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(122-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation7/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2972
end: 3316
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation4/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 1635
end: 1662
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2746
end: 2830
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 1494
end: 1515
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 3399
end: 3419
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2602
end: 2667
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2765
end: 2772
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 1516
end: 1576
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2668
end: 2742
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2816
end: 2824
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2831
end: 2956
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 3980
end: 4804
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 98
end: 1291
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 1581
end: 1634
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 3514
end: 3533
orientation: reverseComplement

L3S3P21
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2530
end: 2582
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 2275
end: 2529
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 1663
end: 2265
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 3346
end: 3398
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 3317
end: 3334
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 1371
end: 1390
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447/1
start: 9
end: 97
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
type: ComponentDefinition
persistent identity: IBMc400
display id: IBMc400
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-190)-PVG-M86N-cat-Plux2-B32-M86C-S-mCherry*(191-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-190)-PVG-M86N-cat-Plux2-B32-M86C-S-mCherry*(191-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ECK120033736
sequence annotation: luxR
sequence annotation: BBa_B0032
sequence annotation: M86C
sequence annotation: cat promoter
sequence annotation: ECK120029600
sequence annotation: PBAD(SapI-)
sequence annotation: mCherry*
sequence annotation: VR
sequence annotation: BBa_B0012
sequence annotation: p15A origin of replication
sequence annotation: L3S2P55
sequence annotation: VF2
sequence annotation: araC
sequence annotation: BBa_B0030
sequence annotation: BBa_J23117
sequence annotation: tetR promoter
sequence annotation: tetR
sequence annotation: RFC10 Suffix
sequence annotation: Plux2
sequence annotation: H6
sequence annotation: RFC10 Prefix (full)
sequence annotation: M86N
sequence annotation: ECK120033737
sequence annotation: cat (BsmBI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400_seq/1

ECK120033736
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5949
end: 6001
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 4664
end: 5419
orientation: reverseComplement

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5595
end: 5607
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5617
end: 5778
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 4468
end: 4565
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 3625
end: 3714
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 2422
end: 2705
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 2735
end: 3309
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 6117
end: 6136
orientation: reverseComplement

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5484
end: 5524
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 6583
end: 7407
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 4574
end: 4630
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 1371
end: 1390
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 1516
end: 2394
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 2714
end: 2728
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5449
end: 5483
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 9
end: 97
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 98
end: 1291
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 6002
end: 6022
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5533
end: 5586
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5782
end: 5919
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5920
end: 5937
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 5426
end: 5440
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 1494
end: 1515
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 3314
end: 3613
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 3723
end: 3779
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400/1
start: 3808
end: 4467
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
type: ComponentDefinition
persistent identity: IBMc331
display id: IBMc331
version: 1
description: pSB1C3(BsmBI-,BsaI+)-BsaI-SrpR(23-192)-BsaI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1C3(BsmBI-,BsaI+)-BsaI-SrpR(23-192)-BsaI Staging plasmid for IBM on SrpR
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1C3 Origin of replication: pMB1 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Prefix (full)
sequence annotation: lambda t0 terminator
sequence annotation: SrpR
sequence annotation: VR
sequence annotation: cat (BsmBI-)
sequence annotation: cat promoter
sequence annotation: rep (pMB1)
sequence annotation: RFC10 Suffix
sequence annotation: VF2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331_seq/1

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 890
end: 911
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 2483
end: 2577
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 919
end: 1430
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 1592
end: 1611
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 1
end: 660
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 661
end: 758
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 1698
end: 2312
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 1438
end: 1458
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331/1
start: 772
end: 791
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
type: ComponentDefinition
persistent identity: IBMc406
display id: IBMc406
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-75)-PDG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-75)-PDG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: SrpR
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECK120029600
sequence annotation: trfA CDS (replication protein)
sequence annotation: PBAD(SapI-)
sequence annotation: M86N
sequence annotation: cat promoter
sequence annotation: cat (BsmBI-)
sequence annotation: pSEVA PS2 Rev
sequence annotation: ECK120033737
sequence annotation: BBa_B0030
sequence annotation: araC
sequence annotation: lambda t0 terminator
sequence annotation: pSEVA PS1 Fwd
sequence annotation: cat
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406_seq/1

SrpR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 1297
end: 1521
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 57
end: 77
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 1842
end: 1931
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 3487
end: 4635
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 984
end: 1267
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 1531
end: 1830
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 2412
end: 2509
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 2025
end: 2165
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 2319
end: 2335
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 1940
end: 1996
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 1276
end: 1290
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 78
end: 956
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 2265
end: 2359
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 5676
end: 5693
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406/1
start: 2510
end: 3169
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
type: ComponentDefinition
persistent identity: IBMc335
display id: IBMc335
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter Expression plasmid for mCherry split at 192/193 inserted with Npu DnaE, caged intein fused with acVHH
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: mCherry*(1-192)
sequence annotation: Caspase3 cut site
sequence annotation: L3S2P55
sequence annotation: BCD1
sequence annotation: NpuN(E52K,E54K,D85K,E89R,E91K)
sequence annotation: p15A origin of replication
sequence annotation: acVHH
sequence annotation: RBS for CDS 1
sequence annotation: PBAD(SapI-)
sequence annotation: tetR promoter
sequence annotation: VF2
sequence annotation: tetR
sequence annotation: cat promoter
sequence annotation: RBS for CDS2
sequence annotation: H6
sequence annotation: ECK120033736
sequence annotation: (NpuC1-30(K2E,R6E,K11E))x2
sequence annotation: RFC10 Prefix (full)
sequence annotation: BBa_B0030
sequence annotation: ECK120033737
sequence annotation: VR
sequence annotation: RFC10 Suffix
sequence annotation: RiboJ
sequence annotation: cat (BsmBI-)
sequence annotation: NpuN(51-102)
sequence annotation: mCherry*(193-236)
sequence annotation: ECK120029600
sequence annotation: NpuC
sequence annotation: araC
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335_seq/1

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 2735
end: 3309
orientation: inline

Caspase3 cut site
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: Caspase3 cut site
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 3644
end: 3655
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 5201
end: 5257
orientation: inline

Caspase3 cut site
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: Caspase3 cut site
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 6272
end: 6283
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 5641
end: 5725
orientation: inline

NpuN(E52K,E54K,D85K,E89R,E91K)
type: SequenceAnnotation
persistent identity: annotation27
display id: annotation27
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: NpuN(E52K,E54K,D85K,E89R,E91K)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation27/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation27/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 3323
end: 3628
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 7211
end: 8035
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation15/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3884
end: 4240
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation16/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 5660
end: 5667
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 2422
end: 2705
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 9
end: 97
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 1371
end: 1390
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 98
end: 1291
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation28
display id: annotation28
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation28/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation28/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 5095
end: 5192
orientation: reverseComplement

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 5711
end: 5719
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation29
display id: annotation29
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation29/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation29/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 6548
end: 6565
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 5729
end: 6085
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 6577
end: 6629
orientation: inline

(NpuC1-30(K2E,R6E,K11E))x2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: (NpuC1-30(K2E,R6E,K11E))x2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 3680
end: 3862
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 1494
end: 1515
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 5277
end: 5560
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation31
display id: annotation31
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation31/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation31/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 2714
end: 2728
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 4350
end: 4406
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 6745
end: 6764
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 6630
end: 6650
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 5566
end: 5640
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation30
display id: annotation30
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation30/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation30/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 4435
end: 5094
orientation: reverseComplement

NpuN(51-102)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: NpuN(51-102)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 6101
end: 6256
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 6416
end: 6547
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 4252
end: 4341
orientation: inline

NpuC
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: NpuC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 6299
end: 6412
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335/1
start: 1516
end: 2394
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
type: ComponentDefinition
persistent identity: IBMc474
display id: IBMc474
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-191)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-191)-Ter Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: mCherry*(1-191)
sequence annotation: VF2
sequence annotation: araC
sequence annotation: VR
sequence annotation: PBAD(SapI-)
sequence annotation: L3S2P55
sequence annotation: BBa_B0030
sequence annotation: ampR
sequence annotation: rep (pMB1)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474_seq/1

mCherry*(1-191)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-191)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 2337
end: 2909
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 982
end: 1001
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 1118
end: 1996
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 3054
end: 3073
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 2024
end: 2307
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 2934
end: 2990
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 2316
end: 2330
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 1
end: 861
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474/1
start: 3160
end: 3774
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
type: ComponentDefinition
persistent identity: IBMc381
display id: IBMc381
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-H6-T Expression plasmid for split mCherry construct as positive control for splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR promoter
sequence annotation: cat (BsmBI-)
sequence annotation: BBa_B0032
sequence annotation: p15A origin of replication
sequence annotation: BBa_B0030
sequence annotation: RFC10 Prefix (full)
sequence annotation: Plux2
sequence annotation: tetR
sequence annotation: L3S2P55
sequence annotation: araC
sequence annotation: VR
sequence annotation: luxR
sequence annotation: VF2
sequence annotation: mCherry*
sequence annotation: ECK120029600
sequence annotation: M86N
sequence annotation: RFC10 Suffix
sequence annotation: ECK120033736
sequence annotation: M86C
sequence annotation: PBAD(SapI-)
sequence annotation: BBa_J23117
sequence annotation: H6
sequence annotation: BBa_B0012
sequence annotation: ECK120033737
sequence annotation: cat promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381_seq/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 9
end: 97
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 3715
end: 4374
orientation: reverseComplement

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5502
end: 5514
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 6583
end: 7407
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 2714
end: 2728
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 1494
end: 1515
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5440
end: 5493
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 98
end: 1291
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 4481
end: 4537
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 1516
end: 2394
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 6117
end: 6136
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 4571
end: 5326
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 1371
end: 1390
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5689
end: 5919
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 3532
end: 3621
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 3221
end: 3520
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 6002
end: 6022
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5949
end: 6001
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5524
end: 5685
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 2422
end: 2705
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5356
end: 5390
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5920
end: 5937
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5391
end: 5431
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 3630
end: 3686
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 4375
end: 4472
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 2735
end: 3211
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381/1
start: 5333
end: 5347
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
type: ComponentDefinition
persistent identity: IBMc175
display id: IBMc175
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86-S-ECF20(102-193)-T Expression plasmid for ECF20 inserted with cis-M86 intein. Activity reference in acVHH-assisted bisection mapping experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Prefix (full)
sequence annotation: VR
sequence annotation: ECF20 102-193
sequence annotation: p15A origin of replication
sequence annotation: ECF20 1-101
sequence annotation: VF2
sequence annotation: M86N
sequence annotation: RFC10 Suffix
sequence annotation: tetR
sequence annotation: araC
sequence annotation: M86C
sequence annotation: ECK120033736
sequence annotation: tetR promoter
sequence annotation: BBa_B0033
sequence annotation: PBAD(SapI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175_seq/1

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 1494
end: 1515
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 3957
end: 3976
orientation: reverseComplement

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 3502
end: 3777
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 4423
end: 5247
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 2731
end: 3033
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 1371
end: 1390
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 3037
end: 3336
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 3842
end: 3862
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 98
end: 1291
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 1516
end: 2394
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 3337
end: 3498
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 3789
end: 3841
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 9
end: 97
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 2714
end: 2724
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175/1
start: 2422
end: 2705
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
type: ComponentDefinition
persistent identity: IBMc322
display id: IBMc322
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GCS-mCherry*(193-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GCS-mCherry*(193-236)-H6-Ter Expression plasmid for mCherry split at 192/193 inserted with extein junction of Mtu RecA
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PBAD(SapI-)
sequence annotation: VR
sequence annotation: mCherry*(193-236)
sequence annotation: H6
sequence annotation: mCherry*(1-192)
sequence annotation: p15A origin of replication
sequence annotation: ECK120033736
sequence annotation: BBa_B0030
sequence annotation: RFC10 Suffix
sequence annotation: araC
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence annotation: VF2
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322_seq/1

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 2422
end: 2705
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 3649
end: 3668
orientation: reverseComplement

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 3320
end: 3451
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 3452
end: 3469
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 2735
end: 3310
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 4115
end: 4939
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 3481
end: 3533
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 2714
end: 2728
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 3534
end: 3554
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 1516
end: 2394
orientation: reverseComplement

tetR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 98
end: 1291
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 1494
end: 1515
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 1371
end: 1390
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322/1
start: 9
end: 97
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
type: ComponentDefinition
persistent identity: IBMc374
display id: IBMc374
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-119)-WDG-ER-LBD-S-M86(120-154)-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-119)-WDG-ER-LBD-S-M86(120-154)-S-ECF20(102-193)-T Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 119/120 by ER-LBD
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR promoter
sequence annotation: ECF20 1-101
sequence annotation: tetR
sequence annotation: PBAD(SapI-)
sequence annotation: M86(120-154)
sequence annotation: ECK120033736
sequence annotation: ER-LBD
sequence annotation: RFC10 Prefix (full)
sequence annotation: M86(1-119)
sequence annotation: BBa_B0033
sequence annotation: RFC10 Suffix
sequence annotation: VR
sequence annotation: ECF20 102-193
sequence annotation: VF2
sequence annotation: araC
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374_seq/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 9
end: 97
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 2731
end: 3033
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 98
end: 1291
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 2422
end: 2705
orientation: inline

M86(120-154)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(120-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 4150
end: 4254
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 4545
end: 4597
orientation: inline

ER-LBD
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ER-LBD
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 3403
end: 4146
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 1494
end: 1515
orientation: inline

M86(1-119)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-119)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 3037
end: 3393
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 2714
end: 2724
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 4598
end: 4618
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 4713
end: 4732
orientation: reverseComplement

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 4258
end: 4533
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 1371
end: 1390
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 1516
end: 2394
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374/1
start: 5179
end: 6003
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
type: ComponentDefinition
persistent identity: IBMc186
display id: IBMc186
version: 1
description: pSB4A3(empty)
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB4A3(empty) Empty plasmid for generating autofluorescence control strains
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB4A3 Origin of replication: pSC101 Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VR
sequence annotation: AmpR
sequence annotation: pSC101 origin
sequence annotation: VF2
sequence annotation: repA
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186_seq/1

VR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
start: 1561
end: 1580
orientation: reverseComplement

AmpR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: AmpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
start: 293
end: 1153
orientation: reverseComplement

pSC101 origin
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: pSC101 origin
multi range type: join
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation0/range1/1
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation0/range0/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation0/range1/1
type: Range
persistent identity: range1
display id: range1
version: 1
position: position0
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
start: 1
end: 292
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation0/range0/1
type: Range
persistent identity: range0
display id: range0
version: 1
position: position1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
start: 1587
end: 3331
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
start: 1273
end: 1292
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: repA
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186/1
start: 1788
end: 2738
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
type: ComponentDefinition
persistent identity: IBMc318
display id: IBMc318
version: 1
description: pSB1A3-BbsI-G-ER-LBD-S-SapI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-BbsI-G-ER-LBD-S-SapI Plasmid carrying Golden Gate substitution insert for ER-LBD domain-insertion mapping
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VF2
sequence annotation: ampR (rev)
sequence annotation: VR
sequence annotation: rep (pMB1)
sequence annotation: ER-LBD
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318_seq/1

VF2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
start: 1040
end: 1059
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (rev)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
start: 59
end: 919
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
start: 2025
end: 2044
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
start: 2131
end: 2745
orientation: reverseComplement

ER-LBD
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ER-LBD
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
start: 1192
end: 1935
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
start: 1947
end: 1967
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318/1
start: 1158
end: 1179
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
type: ComponentDefinition
persistent identity: IBMc452
display id: IBMc452
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(192-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(192-236)-H6-Ter Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR
sequence annotation: H6
sequence annotation: SYNZIP18
sequence annotation: phl2
sequence annotation: PhlF
sequence annotation: L3S3P21
sequence annotation: p15A origin of replication
sequence annotation: VF2
sequence annotation: BCD1
sequence annotation: IOT
sequence annotation: tetR promoter
sequence annotation: RiboJ
sequence annotation: PlacIQ
sequence annotation: PPhlF
sequence annotation: RBS for CDS2
sequence annotation: RFC10 Suffix
sequence annotation: ECK120033736
sequence annotation: mCherry*(192-236)
sequence annotation: RFC10 Prefix (full)
sequence annotation: L3S2P21
sequence annotation: RBS for CDS 1
sequence annotation: VR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452_seq/1

tetR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 98
end: 1291
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 3107
end: 3124
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2831
end: 2956
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 1635
end: 1662
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 1663
end: 2265
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2530
end: 2582
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 3770
end: 4594
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 1371
end: 1390
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2746
end: 2830
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2275
end: 2529
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 9
end: 97
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2668
end: 2742
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 1581
end: 1634
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2602
end: 2667
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2816
end: 2824
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 3189
end: 3209
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 3136
end: 3188
orientation: inline

mCherry*(192-236)
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(192-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2972
end: 3106
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 1494
end: 1515
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 1516
end: 1576
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 2765
end: 2772
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452/1
start: 3304
end: 3323
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
type: ComponentDefinition
persistent identity: IBMc471
display id: IBMc471
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-Ter Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VR
sequence annotation: mCherry*(1-173)
sequence annotation: PBAD(SapI-)
sequence annotation: araC
sequence annotation: L3S2P55
sequence annotation: BBa_B0030
sequence annotation: rep (pMB1)
sequence annotation: VF2
sequence annotation: ampR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471_seq/1

VR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 3000
end: 3019
orientation: reverseComplement

mCherry*(1-173)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-173)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 2337
end: 2855
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 2024
end: 2307
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 1118
end: 1996
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 2880
end: 2936
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 2316
end: 2330
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 3106
end: 3720
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 982
end: 1001
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471/1
start: 1
end: 861
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
type: ComponentDefinition
persistent identity: IBMc382
display id: IBMc382
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-121)-EFG-M86N-cat-Plux2-B32-M86C-S-mCherry*(122-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-121)-EFG-M86N-cat-Plux2-B32-M86C-S-mCherry*(122-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: Plux2
sequence annotation: RFC10 Prefix (full)
sequence annotation: M86C
sequence annotation: BBa_B0030
sequence annotation: mCherry*
sequence annotation: luxR
sequence annotation: M86N
sequence annotation: tetR promoter
sequence annotation: ECK120029600
sequence annotation: cat (BsmBI-)
sequence annotation: araC
sequence annotation: VF2
sequence annotation: tetR
sequence annotation: VR
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033736
sequence annotation: BBa_J23117
sequence annotation: L3S2P55
sequence annotation: p15A origin of replication
sequence annotation: ECK120033737
sequence annotation: H6
sequence annotation: cat promoter
sequence annotation: RFC10 Suffix
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382_seq/1

Plux2
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5326
end: 5379
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 1494
end: 1515
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5410
end: 5571
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5219
end: 5233
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5575
end: 5919
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 4457
end: 5212
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 2735
end: 3097
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 3107
end: 3406
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 9
end: 97
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 3418
end: 3507
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 3601
end: 4260
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 2714
end: 2728
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 1516
end: 2394
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 1371
end: 1390
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 98
end: 1291
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 6117
end: 6136
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 2422
end: 2705
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5949
end: 6001
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5242
end: 5276
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 4367
end: 4423
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 6583
end: 7407
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 3516
end: 3572
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5920
end: 5937
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 4261
end: 4358
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 6002
end: 6022
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5277
end: 5317
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382/1
start: 5388
end: 5400
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
type: ComponentDefinition
persistent identity: IBMc427
display id: IBMc427
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(172-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(172-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PPhlF
sequence annotation: phl2
sequence annotation: L3S3P21
sequence annotation: p15A origin of replication
sequence annotation: VF2
sequence annotation: cat promoter
sequence annotation: RFC10 Prefix (full)
sequence annotation: PlacIQ
sequence annotation: M86C
sequence annotation: tetR
sequence annotation: SrpR
sequence annotation: VR
sequence annotation: tetR promoter
sequence annotation: cat (BsmBI-)
sequence annotation: PhlF
sequence annotation: RiboJ
sequence annotation: BBa_B0032
sequence annotation: RFC10 Suffix
sequence annotation: IOT
sequence annotation: ECK120033736
sequence annotation: L3S2P21
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427_seq/1

PPhlF
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3220
end: 3285
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 2253
end: 2280
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3148
end: 3200
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 4327
end: 5151
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 1371
end: 1390
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 2036
end: 2133
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 1494
end: 1494
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 2199
end: 2252
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3391
end: 3552
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 98
end: 1291
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3556
end: 3681
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3861
end: 3880
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 9
end: 97
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 1517
end: 2035
orientation: reverseComplement

PhlF
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 2281
end: 2883
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3286
end: 3360
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3369
end: 3381
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3746
end: 3766
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 2893
end: 3147
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 3693
end: 3745
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427/1
start: 2134
end: 2194
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
type: ComponentDefinition
persistent identity: IBMc404
display id: IBMc404
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-52)-NGG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-52)-NGG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: trfA CDS (replication protein)
sequence annotation: pSEVA PS1 Fwd
sequence annotation: ECK120033737
sequence annotation: ECK120029600
sequence annotation: cat promoter
sequence annotation: cat (BsmBI-)
sequence annotation: araC
sequence annotation: SrpR
sequence annotation: M86N
sequence annotation: lambda t0 terminator
sequence annotation: RFC10 Prefix (full)
sequence annotation: PBAD(SapI-)
sequence annotation: pSEVA PS2 Rev
sequence annotation: cat
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404_seq/1

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 3418
end: 4566
orientation: reverseComplement

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 5607
end: 5624
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 1871
end: 1927
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 1773
end: 1862
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 2343
end: 2440
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 1956
end: 2096
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 78
end: 956
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 1297
end: 1452
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 1462
end: 1761
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 2196
end: 2290
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 57
end: 77
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 984
end: 1267
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 2250
end: 2266
orientation: reverseComplement

cat
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 2441
end: 3100
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404/1
start: 1276
end: 1290
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
type: ComponentDefinition
persistent identity: IBMc470
display id: IBMc470
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-156)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-156)-Ter Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ampR
sequence annotation: VR
sequence annotation: araC
sequence annotation: rep (pMB1)
sequence annotation: BBa_B0030
sequence annotation: L3S2P55
sequence annotation: PBAD(SapI-)
sequence annotation: VF2
sequence annotation: mCherry*(1-156)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470_seq/1

ampR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 1
end: 861
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 2949
end: 2968
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 1118
end: 1996
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 3055
end: 3669
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 2316
end: 2330
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 2829
end: 2885
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 2024
end: 2307
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 982
end: 1001
orientation: inline

mCherry*(1-156)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-156)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470/1
start: 2337
end: 2804
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
type: ComponentDefinition
persistent identity: IBMc421
display id: IBMc421
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(110-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(110-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: L3S2P21
sequence annotation: PlacIQ
sequence annotation: cat promoter
sequence annotation: M86C
sequence annotation: PhlF
sequence annotation: tetR promoter
sequence annotation: PPhlF
sequence annotation: tetR
sequence annotation: RFC10 Suffix
sequence annotation: p15A origin of replication
sequence annotation: BBa_B0032
sequence annotation: IOT
sequence annotation: ECK120033736
sequence annotation: SrpR
sequence annotation: VR
sequence annotation: VF2
sequence annotation: L3S3P21
sequence annotation: cat (BsmBI-)
sequence annotation: phl2
sequence annotation: RiboJ
sequence annotation: RFC10 Prefix (full)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421_seq/1

L3S2P21
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 2134
end: 2194
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 2199
end: 2252
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 2036
end: 2133
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 3391
end: 3552
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 2281
end: 2883
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 9
end: 97
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 3220
end: 3285
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 98
end: 1291
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 3932
end: 3952
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 4513
end: 5337
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 3369
end: 3381
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 2893
end: 3147
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 3879
end: 3931
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 3556
end: 3867
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 4047
end: 4066
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 1371
end: 1390
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 3148
end: 3200
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 1517
end: 2035
orientation: reverseComplement

phl2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 2253
end: 2280
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 3286
end: 3360
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421/1
start: 1494
end: 1494
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
type: ComponentDefinition
persistent identity: IBMc189
display id: IBMc189
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-5aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-5aa-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-5aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-5aa-ECF20(102-193)-T Expression plasmid for ECF20 spit at 101/102 fused with acVHH via 5 aa linker
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VR
sequence annotation: GGGGS linker
sequence annotation: PBAD(SapI-)
sequence annotation: BBa_B0033
sequence annotation: tetR
sequence annotation: acVHH
sequence annotation: RFC10 Prefix (full)
sequence annotation: RBS for CDS 1
sequence annotation: BCD22
sequence annotation: araC
sequence annotation: ECF20 102-193
sequence annotation: VF2
sequence annotation: cat (BsmBI-)
sequence annotation: RiboJ
sequence annotation: ECK120033737
sequence annotation: cat promoter
sequence annotation: ECK120033736
sequence annotation: ECK120029600
sequence annotation: RBS for 2nd CDS
sequence annotation: tetR promoter
sequence annotation: L3S2P55
sequence annotation: ECF20 1-101
sequence annotation: RFC10 Suffix
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189_seq/1

VR
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 5721
end: 5740
orientation: reverseComplement

GGGGS linker
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: GGGGS linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 5251
end: 5265
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 4442
end: 4725
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 2714
end: 2724
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 98
end: 1291
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 3049
end: 3405
orientation: inline

GGGGS linker
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: GGGGS linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 3034
end: 3048
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 1494
end: 1515
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 4825
end: 4832
orientation: inline

BCD22
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: BCD22
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 4806
end: 4893
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 1516
end: 2394
orientation: reverseComplement

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 5266
end: 5541
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 1371
end: 1390
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 3600
end: 4259
orientation: reverseComplement

RiboJ
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 4731
end: 4805
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 3515
end: 3571
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 4260
end: 4357
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 5553
end: 5605
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 3417
end: 3506
orientation: inline

RBS for 2nd CDS
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: RBS for 2nd CDS
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 4876
end: 4884
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 9
end: 97
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 4366
end: 4422
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 2731
end: 3033
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 2422
end: 2705
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 5606
end: 5626
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 6187
end: 7011
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189/1
start: 4894
end: 5250
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
type: ComponentDefinition
persistent identity: IBMc384
display id: IBMc384
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-158)-DGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(159-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-158)-DGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(159-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0030
sequence annotation: M86C
sequence annotation: ECK120033737
sequence annotation: Plux2
sequence annotation: BBa_B0032
sequence annotation: cat promoter
sequence annotation: araC
sequence annotation: p15A origin of replication
sequence annotation: ECK120033736
sequence annotation: VR
sequence annotation: luxR
sequence annotation: tetR promoter
sequence annotation: BBa_J23117
sequence annotation: mCherry*
sequence annotation: VF2
sequence annotation: cat (BsmBI-)
sequence annotation: RFC10 Suffix
sequence annotation: PBAD(SapI-)
sequence annotation: H6
sequence annotation: ECK120029600
sequence annotation: M86N
sequence annotation: BBa_B0012
sequence annotation: L3S2P55
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384_seq/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 2714
end: 2728
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5521
end: 5682
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 3627
end: 3683
orientation: reverseComplement

Plux2
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5437
end: 5490
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5499
end: 5511
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 4372
end: 4469
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 1516
end: 2394
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 6583
end: 7407
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5949
end: 6001
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 6117
end: 6136
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 4568
end: 5323
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 9
end: 97
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5330
end: 5344
orientation: reverseComplement

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5353
end: 5387
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 2735
end: 3213
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 1371
end: 1390
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 3712
end: 4371
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 6002
end: 6022
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5686
end: 5919
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 2422
end: 2705
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5920
end: 5937
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 3529
end: 3618
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 3218
end: 3517
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 5388
end: 5428
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 4478
end: 4534
orientation: reverseComplement

tetR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 98
end: 1291
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384/1
start: 1494
end: 1515
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
type: ComponentDefinition
persistent identity: IBMc410
display id: IBMc410
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-114)-SGG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-114)-SGG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: SrpR
sequence annotation: M86N
sequence annotation: pSEVA PS1 Fwd
sequence annotation: lambda t0 terminator
sequence annotation: cat promoter
sequence annotation: BBa_B0030
sequence annotation: RFC10 Prefix (full)
sequence annotation: cat (BsmBI-)
sequence annotation: cat
sequence annotation: ECK120029600
sequence annotation: ECK120033737
sequence annotation: PBAD(SapI-)
sequence annotation: trfA CDS (replication protein)
sequence annotation: pSEVA PS2 Rev
sequence annotation: araC
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410_seq/1

SrpR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 1297
end: 1638
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 1648
end: 1947
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 5793
end: 5810
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 2382
end: 2476
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 2529
end: 2626
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 1276
end: 1290
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 57
end: 77
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 2142
end: 2282
orientation: reverseComplement

cat
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 2627
end: 3286
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 1959
end: 2048
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 2057
end: 2113
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 984
end: 1267
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 3604
end: 4752
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 2436
end: 2452
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410/1
start: 78
end: 956
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
type: ComponentDefinition
persistent identity: IBMc456
display id: IBMc456
version: 1
description: pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-112)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(113-207)-HA-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-112)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(113-207)-HA-Ter Expression plasmid for tagged TetR split at 112/113 with SYNZIP17 & SYNZIP18
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3A5* Origin of replication: p15A Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: TetR(2-112)
sequence annotation: BCD1
sequence annotation: VF2
sequence annotation: araC
sequence annotation: PBAD(SapI-)
sequence annotation: RBS for CDS 1
sequence annotation: BBa_B0030
sequence annotation: ampR (BsaI-)
sequence annotation: HA tag
sequence annotation: H6
sequence annotation: p15A origin of replication
sequence annotation: TetR(113-207)
sequence annotation: VR
sequence annotation: SYNZIP18
sequence annotation: RFC10 Suffix
sequence annotation: RBS for CDS2
sequence annotation: RFC10 Prefix (full)
sequence annotation: SYNZIP17
sequence annotation: ECK120033736
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456_seq/1

TetR(2-112)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR(2-112)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 2328
end: 2660
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 2805
end: 2889
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 943
end: 962
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 1088
end: 1966
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 1994
end: 2277
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 2824
end: 2831
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 2286
end: 2300
orientation: inline

ampR (BsaI-)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (BsaI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 3
end: 863
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: HA tag
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 3316
end: 3342
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 2310
end: 2327
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 3988
end: 4812
orientation: inline

TetR(113-207)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR(113-207)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 3031
end: 3315
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 3522
end: 3541
orientation: reverseComplement

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 2890
end: 3015
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 3407
end: 3427
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 2875
end: 2883
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 1066
end: 1087
orientation: inline

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 2676
end: 2801
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456/1
start: 3354
end: 3406
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
type: ComponentDefinition
persistent identity: IBMc458
display id: IBMc458
version: 1
description: pSB1C3(BsmBI-)-BsaI-bla(28-285)-BsaI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1C3(BsmBI-)-BsaI-bla(28-285)-BsaI Staging plasmid for IBM on beta-lactamase
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1C3 Origin of replication: pMB1 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat (BsmBI-)
sequence annotation: lambda t0 terminator
sequence annotation: TEM-1 bla
sequence annotation: cat promoter
sequence annotation: VF2
sequence annotation: VR
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: rep (pMB1)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458_seq/1

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 1
end: 660
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 2745
end: 2839
orientation: reverseComplement

TEM-1 bla
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: TEM-1 bla
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 919
end: 1693
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 661
end: 758
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 772
end: 791
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 1855
end: 1874
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 890
end: 911
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 1701
end: 1721
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458/1
start: 1961
end: 2575
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
type: ComponentDefinition
persistent identity: IBMc370
display id: IBMc370
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-78)-DGG-ER-LBD-S-M86(79-154)-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-78)-DGG-ER-LBD-S-M86(79-154)-S-ECF20(102-193)-T Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 78/79 by ER-LBD
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VR
sequence annotation: ECF20 102-193
sequence annotation: RFC10 Prefix (full)
sequence annotation: ER-LBD
sequence annotation: VF2
sequence annotation: M86(79-154)
sequence annotation: BBa_B0033
sequence annotation: PBAD(SapI-)
sequence annotation: araC
sequence annotation: ECF20 1-101
sequence annotation: tetR promoter
sequence annotation: ECK120033736
sequence annotation: RFC10 Suffix
sequence annotation: p15A origin of replication
sequence annotation: tetR
sequence annotation: M86(1-78)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370_seq/1

VR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 4713
end: 4732
orientation: reverseComplement

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 4258
end: 4533
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 1494
end: 1515
orientation: inline

ER-LBD
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ER-LBD
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 3280
end: 4023
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 1371
end: 1390
orientation: inline

M86(79-154)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(79-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 4027
end: 4254
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 2714
end: 2724
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 2422
end: 2705
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 1516
end: 2394
orientation: reverseComplement

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 2731
end: 3033
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 9
end: 97
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 4545
end: 4597
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 4598
end: 4618
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 5179
end: 6003
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 98
end: 1291
orientation: inline

M86(1-78)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-78)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370/1
start: 3037
end: 3270
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
type: ComponentDefinition
persistent identity: IBMc446
display id: IBMc446
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(160-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(160-236)-H6-Ter Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Prefix (full)
sequence annotation: RBS for CDS2
sequence annotation: L3S3P21
sequence annotation: IOT
sequence annotation: tetR promoter
sequence annotation: mCherry*(160-236)
sequence annotation: SYNZIP18
sequence annotation: L3S2P21
sequence annotation: BCD1
sequence annotation: RBS for CDS 1
sequence annotation: tetR
sequence annotation: H6
sequence annotation: ECK120033736
sequence annotation: RFC10 Suffix
sequence annotation: VF2
sequence annotation: PPhlF
sequence annotation: VR
sequence annotation: RiboJ
sequence annotation: phl2
sequence annotation: PlacIQ
sequence annotation: PhlF
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446_seq/1

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 1494
end: 1515
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2816
end: 2824
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2530
end: 2582
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2275
end: 2529
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 9
end: 97
orientation: inline

mCherry*(160-236)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(160-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2972
end: 3202
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2831
end: 2956
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 1516
end: 1576
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2746
end: 2830
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2765
end: 2772
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 98
end: 1291
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 3203
end: 3220
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 3232
end: 3284
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 3285
end: 3305
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 1371
end: 1390
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2602
end: 2667
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 3400
end: 3419
orientation: reverseComplement

RiboJ
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 2668
end: 2742
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 1635
end: 1662
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 1581
end: 1634
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 1663
end: 2265
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446/1
start: 3866
end: 4690
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
type: ComponentDefinition
persistent identity: IBMc131
display id: IBMc131
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-H6-Ter mCherry expression plasmid. Activity reference in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Suffix
sequence annotation: VF2
sequence annotation: araC
sequence annotation: H6
sequence annotation: VR
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence annotation: mCherry*
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033736
sequence annotation: tetR promoter
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131_seq/1

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 4106
end: 4930
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 3525
end: 3545
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 1371
end: 1390
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 1516
end: 2394
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 3443
end: 3460
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 3640
end: 3659
orientation: reverseComplement

tetR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 98
end: 1291
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 1494
end: 1515
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 2738
end: 3442
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 2422
end: 2705
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 3472
end: 3524
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 9
end: 97
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131/1
start: 2714
end: 2728
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
type: ComponentDefinition
persistent identity: IBMc469
display id: IBMc469
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-121)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-121)-Ter Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0030
sequence annotation: VF2
sequence annotation: PBAD(SapI-)
sequence annotation: rep (pMB1)
sequence annotation: mCherry*(1-121)
sequence annotation: araC
sequence annotation: VR
sequence annotation: L3S2P55
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469_seq/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
start: 2316
end: 2330
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
start: 982
end: 1001
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
start: 2024
end: 2307
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
start: 2950
end: 3564
orientation: reverseComplement

mCherry*(1-121)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-121)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
start: 2337
end: 2699
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation1/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
start: 1118
end: 1996
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
start: 2844
end: 2863
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469/1
start: 2724
end: 2780
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
type: ComponentDefinition
persistent identity: IBMc420
display id: IBMc420
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(78-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(78-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR
sequence annotation: VR
sequence annotation: IOT
sequence annotation: RiboJ
sequence annotation: L3S2P21
sequence annotation: VF2
sequence annotation: tetR promoter
sequence annotation: PPhlF
sequence annotation: cat (BsmBI-)
sequence annotation: RFC10 Suffix
sequence annotation: p15A origin of replication
sequence annotation: M86C
sequence annotation: PlacIQ
sequence annotation: ECK120033736
sequence annotation: L3S3P21
sequence annotation: SrpR
sequence annotation: phl2
sequence annotation: RFC10 Prefix (full)
sequence annotation: BBa_B0032
sequence annotation: PhlF
sequence annotation: cat promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420_seq/1

tetR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 98
end: 1291
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 4143
end: 4162
orientation: reverseComplement

IOT
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 2893
end: 3147
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 3286
end: 3360
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 2134
end: 2194
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 1371
end: 1390
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 9
end: 97
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 3220
end: 3285
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 1517
end: 2035
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 4028
end: 4048
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 4609
end: 5433
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 3391
end: 3552
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 2199
end: 2252
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 3975
end: 4027
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 3148
end: 3200
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 3556
end: 3963
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 2253
end: 2280
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 1494
end: 1494
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 3369
end: 3381
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 2281
end: 2883
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420/1
start: 2036
end: 2133
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
type: ComponentDefinition
persistent identity: IBMc191
display id: IBMc191
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-15aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-15aa-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-15aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-15aa-ECF20(102-193)-T Expression plasmid for ECF20 spit at 101/102 fused with acVHH via 15 aa linker
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ECK120033737
sequence annotation: BBa_B0033
sequence annotation: p15A origin of replication
sequence annotation: VR
sequence annotation: cat promoter
sequence annotation: araC
sequence annotation: acVHH
sequence annotation: ECF20 1-101
sequence annotation: 3x(GGGGS) linker
sequence annotation: L3S2P55
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: PBAD(SapI-)
sequence annotation: VF2
sequence annotation: RBS for CDS 1
sequence annotation: ECK120029600
sequence annotation: RBS for 2nd CDS
sequence annotation: RiboJ
sequence annotation: tetR promoter
sequence annotation: cat (BsmBI-)
sequence annotation: ECK120033736
sequence annotation: ECF20 102-193
sequence annotation: BCD22
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191_seq/1

ECK120033737
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 3545
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BBa_B0033
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version: 1
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label: BBa_B0033
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p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
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label: p15A origin of replication
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start: 6247
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VR
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
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orientation: reverseComplement

cat promoter
type: SequenceAnnotation
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display id: annotation23
version: 1
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ap einfo revcolor: #c6c9d1
label: cat promoter
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orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
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ap einfo revcolor: #75c6a9
label: araC
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start: 1516
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orientation: reverseComplement

acVHH
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
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ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
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start: 4924
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orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation9/range/1

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start: 2731
end: 3033
orientation: inline

3x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: 3x(GGGGS) linker
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation7/range/1

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type: Range
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start: 3034
end: 3078
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation21/range/1

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type: Range
persistent identity: range
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version: 1
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start: 4396
end: 4452
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation18/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 98
end: 1291
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 1494
end: 1515
orientation: inline

3x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 3x(GGGGS) linker
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 5281
end: 5325
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 5666
end: 5686
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 4472
end: 4755
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 1371
end: 1390
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 4855
end: 4862
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 2422
end: 2705
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 3447
end: 3536
orientation: inline

RBS for 2nd CDS
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: RBS for 2nd CDS
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 4906
end: 4914
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 4761
end: 4835
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 9
end: 97
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 3630
end: 4289
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 5613
end: 5665
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 5326
end: 5601
orientation: inline

BCD22
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: BCD22
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 4836
end: 4923
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191/1
start: 3079
end: 3435
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
type: ComponentDefinition
persistent identity: IBMc416
display id: IBMc416
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-176)-QAG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-176)-QAG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ECK120029600
sequence annotation: ECK120033737
sequence annotation: pSEVA PS2 Rev
sequence annotation: cat
sequence annotation: pSEVA PS1 Fwd
sequence annotation: trfA CDS (replication protein)
sequence annotation: M86N
sequence annotation: SrpR
sequence annotation: araC
sequence annotation: BBa_B0030
sequence annotation: cat promoter
sequence annotation: PBAD(SapI-)
sequence annotation: lambda t0 terminator
sequence annotation: RFC10 Prefix (full)
sequence annotation: cat (BsmBI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416_seq/1

ECK120029600
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation12/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 2145
end: 2234
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 2243
end: 2299
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 2622
end: 2638
orientation: reverseComplement

cat
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 2813
end: 3472
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 5979
end: 5996
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 3790
end: 4938
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 1834
end: 2133
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 1297
end: 1824
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 78
end: 956
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 1276
end: 1290
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 2715
end: 2812
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 984
end: 1267
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 2568
end: 2662
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 57
end: 77
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416/1
start: 2328
end: 2468
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
type: ComponentDefinition
persistent identity: IBMc422
display id: IBMc422
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(112-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(112-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Suffix
sequence annotation: tetR
sequence annotation: cat (BsmBI-)
sequence annotation: p15A origin of replication
sequence annotation: PhlF
sequence annotation: L3S2P21
sequence annotation: cat promoter
sequence annotation: VF2
sequence annotation: tetR promoter
sequence annotation: PlacIQ
sequence annotation: PPhlF
sequence annotation: M86C
sequence annotation: phl2
sequence annotation: RFC10 Prefix (full)
sequence annotation: IOT
sequence annotation: BBa_B0032
sequence annotation: SrpR
sequence annotation: ECK120033736
sequence annotation: L3S3P21
sequence annotation: RiboJ
sequence annotation: VR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422_seq/1

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 3926
end: 3946
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 98
end: 1291
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 1517
end: 2035
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 4507
end: 5331
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 2281
end: 2883
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 2134
end: 2194
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 2036
end: 2133
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 1371
end: 1390
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 9
end: 97
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 2199
end: 2252
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 3220
end: 3285
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 3391
end: 3552
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 2253
end: 2280
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 1494
end: 1494
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 2893
end: 3147
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 3369
end: 3381
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 3556
end: 3861
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation16/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 3873
end: 3925
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 3148
end: 3200
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation5/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 3286
end: 3360
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/annotation8/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422/1
start: 4041
end: 4060
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
type: ComponentDefinition
persistent identity: IBMc453
display id: IBMc453
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(193-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(193-236)-H6-Ter Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: L3S3P21
sequence annotation: mCherry*(193-236)
sequence annotation: RBS for CDS 1
sequence annotation: RBS for CDS2
sequence annotation: BCD1
sequence annotation: p15A origin of replication
sequence annotation: RiboJ
sequence annotation: VF2
sequence annotation: PPhlF
sequence annotation: RFC10 Suffix
sequence annotation: tetR promoter
sequence annotation: tetR
sequence annotation: L3S2P21
sequence annotation: PhlF
sequence annotation: RFC10 Prefix (full)
sequence annotation: IOT
sequence annotation: ECK120033736
sequence annotation: H6
sequence annotation: SYNZIP18
sequence annotation: VR
sequence annotation: PlacIQ
sequence annotation: phl2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453_seq/1

L3S3P21
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation15/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2530
end: 2582
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation2/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2972
end: 3103
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2765
end: 2772
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2816
end: 2824
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2746
end: 2830
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 3767
end: 4591
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2668
end: 2742
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 1371
end: 1390
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2602
end: 2667
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 3186
end: 3206
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 9
end: 97
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 98
end: 1291
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 1516
end: 1576
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 1663
end: 2265
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 1494
end: 1515
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2275
end: 2529
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 3133
end: 3185
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 3104
end: 3121
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 2831
end: 2956
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 3301
end: 3320
orientation: reverseComplement

PlacIQ
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 1581
end: 1634
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453/1
start: 1635
end: 1662
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
type: ComponentDefinition
persistent identity: IBMc360
display id: IBMc360
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-M86(1-100)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(101-154)-S-mCherry(193-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-M86(1-100)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(101-154)-S-mCherry(193-236)-H6-T Expression plasmid for mCherry split at 192/193 split by M86 intein split at 100/101 by acVHH
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: 2x(GGGGS) linker
sequence annotation: RiboJ
sequence annotation: araC
sequence annotation: M86C
sequence annotation: H6
sequence annotation: p15A origin of replication
sequence annotation: acVHH
sequence annotation: VF2
sequence annotation: tetR promoter
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033736
sequence annotation: RFC10 Suffix
sequence annotation: BCD1
sequence annotation: L3S2P55
sequence annotation: mCherry*(1-192)
sequence annotation: cat promoter
sequence annotation: BBa_B0030
sequence annotation: cat (BsmBI-)
sequence annotation: RBS for CDS 1
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence annotation: M86N
sequence annotation: ECK120029600
sequence annotation: ECK120033737
sequence annotation: VR
sequence annotation: RBS for CDS2
sequence annotation: mCherry*(193-236)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360_seq/1

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation27
display id: annotation27
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation27/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation27/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 3620
end: 3649
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation3/range/1

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type: Range
persistent identity: range
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version: 1
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start: 5332
end: 5406
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation8/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 1516
end: 2394
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 5882
end: 6043
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 6179
end: 6196
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 6842
end: 7666
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 3650
end: 4006
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 1371
end: 1390
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 9
end: 97
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 5043
end: 5326
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 6208
end: 6260
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 6261
end: 6281
orientation: inline

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 5852
end: 5881
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 2422
end: 2705
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 5407
end: 5491
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 4967
end: 5023
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 2735
end: 3310
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 4861
end: 4958
orientation: reverseComplement

acVHH
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 5495
end: 5851
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 2714
end: 2728
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 4201
end: 4860
orientation: reverseComplement

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 5426
end: 5433
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation29
display id: annotation29
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation29/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation29/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 98
end: 1291
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 1494
end: 1515
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 3314
end: 3613
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 4018
end: 4107
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 4116
end: 4172
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 6376
end: 6395
orientation: reverseComplement

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation28
display id: annotation28
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation28/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation28/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 5477
end: 5485
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360/1
start: 6047
end: 6178
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
type: ComponentDefinition
persistent identity: IBMc097
display id: IBMc097
version: 1
description: pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI Staging plasmid for IBM on mCherry
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1C3 Origin of replication: pMB1 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Prefix (full)
sequence annotation: rep (pMB1)
sequence annotation: VR
sequence annotation: mCherry*
sequence annotation: VF2
sequence annotation: RFC10 Suffix
sequence annotation: cat (BsmBI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097_seq/1

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
start: 1008
end: 1029
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
start: 1880
end: 2494
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
start: 1774
end: 1793
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
start: 1037
end: 1612
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
start: 890
end: 909
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
start: 1620
end: 1640
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097/1
start: 119
end: 778
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
type: ComponentDefinition
persistent identity: IBMc418
display id: IBMc418
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(55-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(55-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0032
sequence annotation: L3S3P21
sequence annotation: IOT
sequence annotation: RFC10 Suffix
sequence annotation: tetR
sequence annotation: M86C
sequence annotation: PlacIQ
sequence annotation: SrpR
sequence annotation: PhlF
sequence annotation: L3S2P21
sequence annotation: VF2
sequence annotation: RiboJ
sequence annotation: RFC10 Prefix (full)
sequence annotation: cat promoter
sequence annotation: ECK120033736
sequence annotation: phl2
sequence annotation: tetR promoter
sequence annotation: cat (BsmBI-)
sequence annotation: PPhlF
sequence annotation: p15A origin of replication
sequence annotation: VR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418_seq/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 3369
end: 3381
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 3148
end: 3200
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 2893
end: 3147
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 4097
end: 4117
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 98
end: 1291
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 3391
end: 3552
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 2199
end: 2252
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 3556
end: 4032
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 2281
end: 2883
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 2134
end: 2194
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 1371
end: 1390
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 3286
end: 3360
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 1494
end: 1494
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 2036
end: 2133
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 4044
end: 4096
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 2253
end: 2280
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 9
end: 97
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 1517
end: 2035
orientation: reverseComplement

PPhlF
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 3220
end: 3285
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 4678
end: 5502
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418/1
start: 4212
end: 4231
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
type: ComponentDefinition
persistent identity: IBMc391
display id: IBMc391
version: 1
description: pSEVA321
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321 Empty plasmid for generating autofluorescence control strains
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat promoter
sequence annotation: cat
sequence annotation: trfA CDS (replication protein)
sequence annotation: lambda t0 terminator
sequence annotation: pSEVA PS1 Fwd
sequence annotation: pSEVA PS2 Rev
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391_seq/1

cat promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
start: 302
end: 399
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
start: 400
end: 1059
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
start: 1377
end: 2525
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
start: 155
end: 249
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
start: 3566
end: 3583
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391/1
start: 209
end: 225
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
type: ComponentDefinition
persistent identity: IBMc478
display id: IBMc478
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-156)-5aa-SYNZIP17-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-156)-5aa-SYNZIP17-Ter Expression plasmid for mCherry N-lobe-SYNZIP17. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: rep (pMB1)
sequence annotation: PBAD(SapI-)
sequence annotation: L3S2P55
sequence annotation: mCherry*(1-156)
sequence annotation: VR
sequence annotation: ampR
sequence annotation: VF2
sequence annotation: araC
sequence annotation: BBa_B0030
sequence annotation: SYNZIP17
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478_seq/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 3196
end: 3810
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 2024
end: 2307
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 2970
end: 3026
orientation: inline

mCherry*(1-156)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-156)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 2340
end: 2804
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 3090
end: 3109
orientation: reverseComplement

ampR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 1
end: 861
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 982
end: 1001
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 1118
end: 1996
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 2316
end: 2330
orientation: inline

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478/1
start: 2820
end: 2945
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
type: ComponentDefinition
persistent identity: IBMc369
display id: IBMc369
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-77)-IDG-ER-LBD-S-M86(78-154)-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-77)-IDG-ER-LBD-S-M86(78-154)-S-ECF20(102-193)-T Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 77/78 by ER-LBD
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: BBa_B0033
sequence annotation: ECF20 1-101
sequence annotation: VF2
sequence annotation: M86(78-154)
sequence annotation: tetR promoter
sequence annotation: PBAD(SapI-)
sequence annotation: p15A origin of replication
sequence annotation: ECK120033736
sequence annotation: araC
sequence annotation: M86(1-77)
sequence annotation: VR
sequence annotation: ER-LBD
sequence annotation: ECF20 102-193
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369_seq/1

tetR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 98
end: 1291
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 1494
end: 1515
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 4598
end: 4618
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 2714
end: 2724
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 2731
end: 3033
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 1371
end: 1390
orientation: inline

M86(78-154)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(78-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 4024
end: 4254
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 9
end: 97
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 2422
end: 2705
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 5179
end: 6003
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 4545
end: 4597
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 1516
end: 2394
orientation: reverseComplement

M86(1-77)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-77)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 3037
end: 3267
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 4713
end: 4732
orientation: reverseComplement

ER-LBD
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ER-LBD
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 3277
end: 4020
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369/1
start: 4258
end: 4533
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
type: ComponentDefinition
persistent identity: IBMc480
display id: IBMc480
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-176)-5aa-SYNZIP17-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-176)-5aa-SYNZIP17-Ter Expression plasmid for mCherry N-lobe-SYNZIP17. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PBAD(SapI-)
sequence annotation: rep (pMB1)
sequence annotation: mCherry*(1-176)
sequence annotation: L3S2P55
sequence annotation: VR
sequence annotation: VF2
sequence annotation: BBa_B0030
sequence annotation: ampR
sequence annotation: SYNZIP17
sequence annotation: araC
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480_seq/1

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 2024
end: 2307
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 3256
end: 3870
orientation: reverseComplement

mCherry*(1-176)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-176)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 2340
end: 2864
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 3030
end: 3086
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 3150
end: 3169
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 982
end: 1001
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 2316
end: 2330
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 1
end: 861
orientation: reverseComplement

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 2880
end: 3005
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480/1
start: 1118
end: 1996
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
type: ComponentDefinition
persistent identity: IBMc392
display id: IBMc392
version: 1
description: pSB3K3-TT-PBAD-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3K3-TT-PBAD-T Empty plasmid for generating autofluorescence control strains
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3K3 Origin of replication: p15A Selection Markers: Kanamycin
owned by: trevorho
date: 17-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence annotation: kanR
sequence annotation: p15A origin of replication
sequence annotation: VR
sequence annotation: VF2
sequence annotation: araC
sequence annotation: BBa_B0015
sequence annotation: L3S3P21
sequence annotation: L3S2P11
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392_seq/1

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 2809
end: 2829
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 1334
end: 1355
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: kanR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 3045
end: 3860
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
multi range type: join
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation6/range1/1
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation6/range0/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation6/range1/1
type: Range
persistent identity: range1
display id: range1
version: 1
position: position0
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 1
end: 815
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation6/range0/1
type: Range
persistent identity: range0
display id: range0
version: 1
position: position1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 4192
end: 4201
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 2963
end: 2982
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 1216
end: 1235
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 1482
end: 2360
orientation: reverseComplement

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0015
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 2680
end: 2808
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 1356
end: 1408
orientation: inline

L3S2P11
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: L3S2P11
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392/1
start: 1417
end: 1473
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
type: ComponentDefinition
persistent identity: IBMc368
display id: IBMc368
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-76)-TIG-ER-LBD-S-M86(77-154)-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-76)-TIG-ER-LBD-S-M86(77-154)-S-ECF20(102-193)-T Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 76/77 by ER-LBD
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PBAD(SapI-)
sequence annotation: RFC10 Prefix (full)
sequence annotation: VF2
sequence annotation: RFC10 Suffix
sequence annotation: araC
sequence annotation: ECK120033736
sequence annotation: ER-LBD
sequence annotation: tetR promoter
sequence annotation: BBa_B0033
sequence annotation: M86(1-76)
sequence annotation: ECF20 102-193
sequence annotation: tetR
sequence annotation: M86(77-154)
sequence annotation: ECF20 1-101
sequence annotation: VR
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368_seq/1

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 2422
end: 2705
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 1494
end: 1515
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 1371
end: 1390
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 4598
end: 4618
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 1516
end: 2394
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 4545
end: 4597
orientation: inline

ER-LBD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ER-LBD
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 3274
end: 4017
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 9
end: 97
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 2714
end: 2724
orientation: inline

M86(1-76)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-76)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 3037
end: 3264
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 4258
end: 4533
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 98
end: 1291
orientation: inline

M86(77-154)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(77-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 4021
end: 4254
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 2731
end: 3033
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 4713
end: 4732
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368/1
start: 5179
end: 6003
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
type: ComponentDefinition
persistent identity: IBMc050
display id: IBMc050
version: 1
description: pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI Plasmid carrying Golden Gate substitution insert for IBM, M86 intein, using Plux2 as C-lobe promoter
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicillin / Chloramphenicol
owned by: trevorho
date: 09-OCT-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_J23117
sequence annotation: luxR
sequence annotation: cat (BsmBI-)
sequence annotation: cat promoter
sequence annotation: M86N
sequence annotation: VF2
sequence annotation: VR
sequence annotation: ECK120029600
sequence annotation: L3S2P55
sequence annotation: BBa_B0030
sequence annotation: ampR (rev)
sequence annotation: M86C
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECK120033737
sequence annotation: RFC10 Suffix
sequence annotation: Plux2
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: rep (pMB1)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050_seq/1

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3269
end: 3303
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 2484
end: 3239
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 1628
end: 2287
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 2288
end: 2385
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 1134
end: 1433
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 982
end: 1001
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3688
end: 3707
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 1445
end: 1534
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 2394
end: 2450
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3246
end: 3260
orientation: reverseComplement

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (rev)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 1
end: 861
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3437
end: 3598
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 1100
end: 1121
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 1543
end: 1599
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3610
end: 3630
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3353
end: 3406
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3415
end: 3427
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3304
end: 3344
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050/1
start: 3794
end: 4408
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
type: ComponentDefinition
persistent identity: IBMc479
display id: IBMc479
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-5aa-SYNZIP17-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-5aa-SYNZIP17-Ter Expression plasmid for mCherry N-lobe-SYNZIP17. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: L3S2P55
sequence annotation: araC
sequence annotation: mCherry*(1-173)
sequence annotation: SYNZIP17
sequence annotation: ampR
sequence annotation: VF2
sequence annotation: PBAD(SapI-)
sequence annotation: VR
sequence annotation: BBa_B0030
sequence annotation: rep (pMB1)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479_seq/1

L3S2P55
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 3021
end: 3077
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 1118
end: 1996
orientation: reverseComplement

mCherry*(1-173)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-173)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 2337
end: 2855
orientation: inline

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 2871
end: 2996
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 1
end: 861
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 982
end: 1001
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 2024
end: 2307
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 3141
end: 3160
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 2316
end: 2330
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479/1
start: 3247
end: 3861
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
type: ComponentDefinition
persistent identity: IBMc259
display id: IBMc259
version: 1
description: pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter TetR reporter plasmid
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA471 Origin of replication: pSC101 Selection Markers: Spectinomycin
owned by: trevorho
date: 09-OCT-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Prefix (full)
sequence annotation: SmR
sequence annotation: mScarlet-I
sequence annotation: pSEVA PS2 Rev
sequence annotation: RiboJ10
sequence annotation: RFC10 Suffix
sequence annotation: BBa_B0064
sequence annotation: rrnB T1 terminator
sequence annotation: L3S2P21
sequence annotation: pSEVA PS1 Fwd
sequence annotation: lambda t0 terminator
sequence annotation: pTet*
sequence annotation: Rep101
sequence annotation: pSC101 ori
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259_seq/1

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 2877
end: 2898
orientation: inline

SmR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: SmR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 186
end: 977
orientation: inline

mScarlet-I
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: mScarlet-I
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 3095
end: 3790
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 3934
end: 3950
orientation: reverseComplement

RiboJ10
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
feature type: insulator
label: RiboJ10
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 2994
end: 3068
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 3855
end: 3875
orientation: inline

BBa_B0064
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: BBa_B0064
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 3077
end: 3088
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: rrnB T1 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 2729
end: 2815
orientation: reverseComplement

L3S2P21
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 3794
end: 3854
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 2719
end: 2736
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 3880
end: 3974
orientation: inline

pTet*
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: pTet*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 2918
end: 2993
orientation: inline

Rep101
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #993366
ap einfo revcolor: #993366
label: Rep101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 1242
end: 2192
orientation: reverseComplement

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: pSC101 ori
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259/1
start: 2240
end: 2462
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
type: ComponentDefinition
persistent identity: IBMc120
display id: IBMc120
version: 1
description: pSB4A3-PECF20-B30-mScarlet-I-B15
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB4A3-PECF20-B30-mScarlet-I-B15 ECF20 reporter plasmid
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB4A3 Origin of replication: pSC101 Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: VF2
sequence annotation: pSC101 origin
sequence annotation: BBa_B0015
sequence annotation: BBa_B0030
sequence annotation: 2ndVal-mScarlet-I
sequence annotation: repA
sequence annotation: VR
sequence annotation: PECF20
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120_seq/1

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 1540
end: 1561
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 2490
end: 2510
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 1422
end: 1441
orientation: inline

pSC101 origin
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: pSC101 origin
multi range type: join
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation4/range0/1
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation4/range1/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation4/range0/1
type: Range
persistent identity: range0
display id: range0
version: 1
position: position1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 2670
end: 4265
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation4/range1/1
type: Range
persistent identity: range1
display id: range1
version: 1
position: position0
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 1
end: 441
orientation: reverseComplement

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0015
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 2361
end: 2489
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 1630
end: 1644
orientation: inline

2ndVal-mScarlet-I
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 2ndVal-mScarlet-I
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 1651
end: 2349
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: repA
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 2871
end: 3821
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 2644
end: 2663
orientation: reverseComplement

PECF20
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: PECF20
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120/1
start: 1562
end: 1621
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
type: ComponentDefinition
persistent identity: IBMc323
display id: IBMc323
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEYCFNS-mCherry*(193-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEYCFNS-mCherry*(193-236)-H6-Ter Expression plasmid for mCherry split at 192/193 inserted with extein junction of Npu DnaE
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: araC
sequence annotation: mCherry*(193-236)
sequence annotation: BBa_B0030
sequence annotation: tetR
sequence annotation: ECK120033736
sequence annotation: RFC10 Suffix
sequence annotation: PBAD(SapI-)
sequence annotation: p15A origin of replication
sequence annotation: VR
sequence annotation: tetR promoter
sequence annotation: H6
sequence annotation: VF2
sequence annotation: RFC10 Prefix (full)
sequence annotation: mCherry*(1-192)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323_seq/1

araC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 1516
end: 2394
orientation: reverseComplement

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 3335
end: 3466
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 2714
end: 2728
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 98
end: 1291
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 3496
end: 3548
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 3549
end: 3569
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 2422
end: 2705
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 4130
end: 4954
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 3664
end: 3683
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 9
end: 97
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 3467
end: 3484
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 1371
end: 1390
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 1494
end: 1515
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323/1
start: 2735
end: 3310
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
type: ComponentDefinition
persistent identity: IBMc379
display id: IBMc379
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-22)-LG-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(23-154)-S-mCherry*(193-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-22)-LG-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(23-154)-S-mCherry*(193-236)-H6-T Expression plasmid for mCherry split at 192/193 split by M86 intein split at 22/23 by acVHH
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RBS for CDS 1
sequence annotation: PBAD(SapI-)
sequence annotation: M86(23-154)
sequence annotation: cat promoter
sequence annotation: cat (BsmBI-)
sequence annotation: ECK120033737
sequence annotation: BBa_B0030
sequence annotation: acVHH
sequence annotation: tetR
sequence annotation: araC
sequence annotation: 2x(GGGGS) linker
sequence annotation: mCherry*(1-192)
sequence annotation: L3S2P55
sequence annotation: RBS for CDS2
sequence annotation: RFC10 Prefix (full)
sequence annotation: VF2
sequence annotation: M86(1-22)
sequence annotation: tetR promoter
sequence annotation: H6
sequence annotation: VR
sequence annotation: p15A origin of replication
sequence annotation: ECK120033736
sequence annotation: mCherry*(193-236)
sequence annotation: BCD1
sequence annotation: RFC10 Suffix
sequence annotation: ECK120029600
sequence annotation: RiboJ
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379_seq/1

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 5184
end: 5191
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 4801
end: 5084
orientation: inline

M86(23-154)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(23-154)
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 5640
end: 6035
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 4619
end: 4716
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 3959
end: 4618
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 3874
end: 3930
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 2706
end: 2720
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 5253
end: 5609
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 2414
end: 2697
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 90
end: 1283
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 1508
end: 2386
orientation: reverseComplement

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 3378
end: 3407
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 2727
end: 3302
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 4725
end: 4781
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 5235
end: 5243
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation29
display id: annotation29
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation29/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation29/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 1486
end: 1507
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 3408
end: 3764
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 1363
end: 1382
orientation: inline

M86(1-22)
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-22)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 3306
end: 3371
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 1
end: 89
orientation: inline

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 5610
end: 5639
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 6171
end: 6188
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation28
display id: annotation28
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation28/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation28/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 6368
end: 6387
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 6834
end: 7658
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 6200
end: 6252
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 6039
end: 6170
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 5165
end: 5249
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 6253
end: 6273
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 3776
end: 3865
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation27
display id: annotation27
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation27/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/annotation27/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379/1
start: 5090
end: 5164
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
type: ComponentDefinition
persistent identity: IBMc475
display id: IBMc475
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-192)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-192)-Ter Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ampR
sequence annotation: BBa_B0030
sequence annotation: L3S2P55
sequence annotation: VF2
sequence annotation: PBAD(SapI-)
sequence annotation: mCherry*(1-192)
sequence annotation: araC
sequence annotation: rep (pMB1)
sequence annotation: VR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475_seq/1

ampR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 1
end: 861
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 2316
end: 2330
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 2937
end: 2993
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 982
end: 1001
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 2024
end: 2307
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 2337
end: 2912
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 1118
end: 1996
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 3163
end: 3777
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475/1
start: 3057
end: 3076
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
type: ComponentDefinition
persistent identity: IBMc078
display id: IBMc078
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-27)-BsaI-BsaI-mCherry*(218-236)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-27)-BsaI-BsaI-mCherry*(218-236)-T Expression vector to generate ORF insertion library in IBM on mCherry
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR promoter
sequence annotation: araC
sequence annotation: ECK120033736
sequence annotation: PBAD(SapI-)
sequence annotation: RFC10 Prefix (full)
sequence annotation: spacing sequence
sequence annotation: RFC10 Suffix
sequence annotation: p15A origin of replication
sequence annotation: mCherry*
sequence annotation: tetR
sequence annotation: BBa_B0030
sequence annotation: VR
sequence annotation: VF2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078_seq/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 125
end: 213
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 1632
end: 2510
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 3032
end: 3084
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 2538
end: 2821
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 1610
end: 1631
orientation: inline

spacing sequence
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: spacing sequence
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 2940
end: 2955
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 3085
end: 3105
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 3666
end: 4490
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 2854
end: 2932
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 214
end: 1407
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 2963
end: 3020
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 2830
end: 2844
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 3200
end: 3219
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078/1
start: 1487
end: 1506
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
type: ComponentDefinition
persistent identity: IBMc176
display id: IBMc176
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-ECF20(102-193)-T Expression vector to generate ORF insertion library in acVHH-assisted bisection mapping experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: M86N
sequence annotation: M86C
sequence annotation: PBAD(SapI-)
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECF20 1-101
sequence annotation: VF2
sequence annotation: ECF20 102-193
sequence annotation: RFC10 Suffix
sequence annotation: tetR
sequence annotation: VR
sequence annotation: ECK120033736
sequence annotation: araC
sequence annotation: spacing sequence
sequence annotation: p15A origin of replication
sequence annotation: tetR promoter
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176_seq/1

M86N
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 3037
end: 3055
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 3086
end: 3104
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 2422
end: 2705
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 1494
end: 1515
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 2731
end: 3033
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 1371
end: 1390
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 3108
end: 3383
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 3448
end: 3468
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 98
end: 1291
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 3563
end: 3582
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 3395
end: 3447
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 1516
end: 2394
orientation: reverseComplement

spacing sequence
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: spacing sequence
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 3063
end: 3078
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 4029
end: 4853
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 9
end: 97
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176/1
start: 2714
end: 2724
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
type: ComponentDefinition
persistent identity: IBMc339
display id: IBMc339
version: 1
description: pSEVA171
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA171 Empty plasmid for generating autofluorescence control strains
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA171 Origin of replication: pSC101 Selection Markers: Ampicillin
owned by: trevorho
date: 11-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: pSEVA PS2 Rev
sequence annotation: rrnB T1 terminator
sequence annotation: pSEVA PS1 Fwd
sequence annotation: Rep101
sequence annotation: lambda t0 terminator
sequence annotation: fd terminator
sequence annotation: pSC101 ori
sequence annotation: AmpR
sequence annotation: AmpR promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339_seq/1

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 55
end: 71
orientation: reverseComplement

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: rrnB T1 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 2924
end: 3010
orientation: reverseComplement

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 2914
end: 2931
orientation: inline

Rep101
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #993366
ap einfo revcolor: #993366
label: Rep101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 1437
end: 2387
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 1
end: 95
orientation: inline

fd terminator
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: fd terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 1132
end: 1171
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: pSC101 ori
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 2435
end: 2657
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: AmpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 268
end: 1128
orientation: inline

AmpR promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: AmpR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339/1
start: 176
end: 267
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
type: ComponentDefinition
persistent identity: IBMc385
display id: IBMc385
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-160)-ALG-M86N-cat-Plux2-B32-M86C-S-mCherry*(161-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-160)-ALG-M86N-cat-Plux2-B32-M86C-S-mCherry*(161-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR
sequence annotation: H6
sequence annotation: L3S2P55
sequence annotation: ECK120033736
sequence annotation: BBa_B0032
sequence annotation: M86C
sequence annotation: mCherry*
sequence annotation: VR
sequence annotation: BBa_B0012
sequence annotation: Plux2
sequence annotation: BBa_B0030
sequence annotation: araC
sequence annotation: BBa_J23117
sequence annotation: tetR promoter
sequence annotation: M86N
sequence annotation: VF2
sequence annotation: cat (BsmBI-)
sequence annotation: luxR
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: ECK120029600
sequence annotation: cat promoter
sequence annotation: p15A origin of replication
sequence annotation: ECK120033737
sequence annotation: PBAD(SapI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385_seq/1

tetR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 98
end: 1291
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 5923
end: 5940
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 4484
end: 4540
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 5952
end: 6004
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 5505
end: 5517
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 5527
end: 5688
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation5/range/1

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start: 5692
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VR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation4/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 6120
end: 6139
orientation: reverseComplement

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation16/range/1

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version: 1
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start: 5394
end: 5434
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation15/range/1

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version: 1
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start: 2735
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Plux2
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation14/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 5443
end: 5496
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation17/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2714
end: 2728
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation25/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 1516
end: 2394
orientation: reverseComplement

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation18/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 5359
end: 5393
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation21/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 9
end: 97
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation12/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 3224
end: 3523
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 1371
end: 1390
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 3718
end: 4377
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 4574
end: 5329
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 1494
end: 1515
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 6005
end: 6025
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 3535
end: 3624
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 4378
end: 4475
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 6586
end: 7410
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 3633
end: 3689
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 5336
end: 5350
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385/1
start: 2422
end: 2705
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
type: ComponentDefinition
persistent identity: IBMc396
display id: IBMc396
version: 1
description: pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat
sequence annotation: 3x(GGGGS) linker
sequence annotation: 29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T
sequence annotation: pSEVA PS1 Fwd
sequence annotation: BBa_B0030
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033736
sequence annotation: araC
sequence annotation: trfA CDS (replication protein)
sequence annotation: RFC10 Prefix (full)
sequence annotation: acVHH
sequence annotation: RFC10 Suffix
sequence annotation: H6
sequence annotation: pSEVA PS2 Rev
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396_seq/1

cat
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 2638
end: 3297
orientation: inline

3x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: 3x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 1852
end: 1896
orientation: inline

29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: 29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 1318
end: 1851
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 5804
end: 5821
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 1276
end: 1290
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 984
end: 1267
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 2265
end: 2317
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 78
end: 956
orientation: reverseComplement

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 3615
end: 4763
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 56
end: 77
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 1897
end: 2253
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 2318
end: 2338
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 1300
end: 1317
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396/1
start: 2447
end: 2463
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
type: ComponentDefinition
persistent identity: IBMc190
display id: IBMc190
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-10aa-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-10aa-ECF20(102-193)-T Expression plasmid for ECF20 spit at 101/102 fused with acVHH via 10 aa linker
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0033
sequence annotation: cat (BsmBI-)
sequence annotation: acVHH
sequence annotation: VF2
sequence annotation: 2x(GGGGS) linker
sequence annotation: VR
sequence annotation: ECF20 1-101
sequence annotation: ECF20 102-193
sequence annotation: ECK120033736
sequence annotation: ECK120029600
sequence annotation: RBS for CDS 1
sequence annotation: RiboJ
sequence annotation: RFC10 Suffix
sequence annotation: cat promoter
sequence annotation: p15A origin of replication
sequence annotation: tetR promoter
sequence annotation: RBS for 2nd CDS
sequence annotation: PBAD(SapI-)
sequence annotation: araC
sequence annotation: RFC10 Prefix (full)
sequence annotation: tetR
sequence annotation: L3S2P55
sequence annotation: ECK120033737
sequence annotation: BCD22
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190_seq/1

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 2714
end: 2724
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 3615
end: 4274
orientation: reverseComplement

acVHH
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 3064
end: 3420
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 1371
end: 1390
orientation: inline

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 3034
end: 3063
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 5751
end: 5770
orientation: reverseComplement

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 2731
end: 3033
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 5296
end: 5571
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 5583
end: 5635
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 3432
end: 3521
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 4840
end: 4847
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 4746
end: 4820
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 5636
end: 5656
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 4275
end: 4372
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 6217
end: 7041
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 9
end: 97
orientation: inline

RBS for 2nd CDS
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: RBS for 2nd CDS
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 4891
end: 4899
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 2422
end: 2705
orientation: inline

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 5266
end: 5295
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 1516
end: 2394
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 1494
end: 1515
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 4909
end: 5265
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 4457
end: 4740
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 98
end: 1291
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 4381
end: 4437
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 3530
end: 3586
orientation: reverseComplement

BCD22
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: BCD22
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190/1
start: 4821
end: 4908
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
type: ComponentDefinition
persistent identity: IBMc477
display id: IBMc477
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-121)-5aa-SYNZIP17-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-121)-5aa-SYNZIP17-Ter Expression plasmid for mCherry N-lobe-SYNZIP17. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: rep (pMB1)
sequence annotation: ampR
sequence annotation: araC
sequence annotation: VF2
sequence annotation: mCherry*(1-121)
sequence annotation: BBa_B0030
sequence annotation: PBAD(SapI-)
sequence annotation: VR
sequence annotation: L3S2P55
sequence annotation: SYNZIP17
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477_seq/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 3091
end: 3705
orientation: reverseComplement

ampR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 1
end: 861
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 1118
end: 1996
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 982
end: 1001
orientation: inline

mCherry*(1-121)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-121)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 2337
end: 2699
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 2316
end: 2330
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 2024
end: 2307
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 2985
end: 3004
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 2865
end: 2921
orientation: inline

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477/1
start: 2715
end: 2840
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
type: ComponentDefinition
persistent identity: IBMc409
display id: IBMc409
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-111)-LDG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-111)-LDG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: SrpR
sequence annotation: araC
sequence annotation: pSEVA PS2 Rev
sequence annotation: M86N
sequence annotation: BBa_B0030
sequence annotation: PBAD(SapI-)
sequence annotation: pSEVA PS1 Fwd
sequence annotation: ECK120033737
sequence annotation: cat promoter
sequence annotation: ECK120029600
sequence annotation: cat
sequence annotation: lambda t0 terminator
sequence annotation: RFC10 Prefix (full)
sequence annotation: trfA CDS (replication protein)
sequence annotation: cat (BsmBI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409_seq/1

SrpR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 1297
end: 1629
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 78
end: 956
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 2427
end: 2443
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 1639
end: 1938
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 1276
end: 1290
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 984
end: 1267
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 5784
end: 5801
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 2048
end: 2104
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 2520
end: 2617
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 1950
end: 2039
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 2618
end: 3277
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 2373
end: 2467
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 57
end: 77
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 3595
end: 4743
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409/1
start: 2133
end: 2273
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
type: ComponentDefinition
persistent identity: IBMc455
display id: IBMc455
version: 1
description: pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-70)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(71-207)-HA-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-70)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(71-207)-HA-Ter Expression plasmid for tagged TetR split at 70/71 with SYNZIP17 & SYNZIP18
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3A5* Origin of replication: p15A Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: SYNZIP17
sequence annotation: ampR (BsaI-)
sequence annotation: TetR(2-70)
sequence annotation: SYNZIP18
sequence annotation: ECK120033736
sequence annotation: VR
sequence annotation: PBAD(SapI-)
sequence annotation: RBS for CDS 1
sequence annotation: BBa_B0030
sequence annotation: TetR(71-207)
sequence annotation: HA tag
sequence annotation: RFC10 Suffix
sequence annotation: VF2
sequence annotation: BCD1
sequence annotation: RBS for CDS2
sequence annotation: araC
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Prefix (full)
sequence annotation: H6
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455_seq/1

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2550
end: 2675
orientation: inline

ampR (BsaI-)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (BsaI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 3
end: 863
orientation: inline

TetR(2-70)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR(2-70)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2328
end: 2534
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2764
end: 2889
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 3354
end: 3406
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 3522
end: 3541
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 1994
end: 2277
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2698
end: 2705
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2286
end: 2300
orientation: inline

TetR(71-207)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: TetR(71-207)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2905
end: 3315
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: HA tag
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 3316
end: 3342
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 3407
end: 3427
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 943
end: 962
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2679
end: 2763
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2749
end: 2757
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 1088
end: 1966
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 3988
end: 4812
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 1066
end: 1087
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455/1
start: 2310
end: 2327
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
type: ComponentDefinition
persistent identity: IBMc408
display id: IBMc408
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-109)-QGG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-109)-QGG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat (BsmBI-)
sequence annotation: lambda t0 terminator
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECK120029600
sequence annotation: BBa_B0030
sequence annotation: M86N
sequence annotation: cat
sequence annotation: pSEVA PS2 Rev
sequence annotation: trfA CDS (replication protein)
sequence annotation: SrpR
sequence annotation: cat promoter
sequence annotation: PBAD(SapI-)
sequence annotation: pSEVA PS1 Fwd
sequence annotation: ECK120033737
sequence annotation: araC
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408_seq/1

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 2127
end: 2267
orientation: reverseComplement

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 2367
end: 2461
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 57
end: 77
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 1944
end: 2033
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 1276
end: 1290
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 1633
end: 1932
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 2612
end: 3271
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 2421
end: 2437
orientation: reverseComplement

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 3589
end: 4737
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 1297
end: 1623
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 2514
end: 2611
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 984
end: 1267
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 5778
end: 5795
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 2042
end: 2098
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408/1
start: 78
end: 956
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
type: ComponentDefinition
persistent identity: IBMc417
display id: IBMc417
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(53-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(53-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VF2
sequence annotation: ECK120033736
sequence annotation: SrpR
sequence annotation: IOT
sequence annotation: RiboJ
sequence annotation: PPhlF
sequence annotation: PhlF
sequence annotation: L3S2P21
sequence annotation: RFC10 Prefix (full)
sequence annotation: cat promoter
sequence annotation: VR
sequence annotation: tetR promoter
sequence annotation: PlacIQ
sequence annotation: BBa_B0032
sequence annotation: M86C
sequence annotation: tetR
sequence annotation: p15A origin of replication
sequence annotation: L3S3P21
sequence annotation: phl2
sequence annotation: RFC10 Suffix
sequence annotation: cat (BsmBI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417_seq/1

VF2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 1371
end: 1390
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 4050
end: 4102
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 3556
end: 4038
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 2893
end: 3147
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 3286
end: 3360
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 3220
end: 3285
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 2281
end: 2883
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 2134
end: 2194
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 1494
end: 1494
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 2036
end: 2133
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 4218
end: 4237
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 9
end: 97
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 2199
end: 2252
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 3369
end: 3381
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 3391
end: 3552
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 98
end: 1291
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 4684
end: 5508
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 3148
end: 3200
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 2253
end: 2280
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 4103
end: 4123
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417/1
start: 1517
end: 2035
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
type: ComponentDefinition
persistent identity: IBMc411
display id: IBMc411
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-116)-LIG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-116)-LIG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: lambda t0 terminator
sequence annotation: trfA CDS (replication protein)
sequence annotation: pSEVA PS1 Fwd
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120029600
sequence annotation: cat (BsmBI-)
sequence annotation: pSEVA PS2 Rev
sequence annotation: araC
sequence annotation: ECK120033737
sequence annotation: cat
sequence annotation: SrpR
sequence annotation: BBa_B0030
sequence annotation: RFC10 Prefix (full)
sequence annotation: cat promoter
sequence annotation: M86N
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411_seq/1

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 2388
end: 2482
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 3610
end: 4758
orientation: reverseComplement

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 5799
end: 5816
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 984
end: 1267
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 1965
end: 2054
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 2148
end: 2288
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 2442
end: 2458
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 78
end: 956
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 2063
end: 2119
orientation: reverseComplement

cat
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 2633
end: 3292
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 1297
end: 1644
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 1276
end: 1290
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 57
end: 77
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 2535
end: 2632
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411/1
start: 1654
end: 1953
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
type: ComponentDefinition
persistent identity: IBMc180
display id: IBMc180
version: 1
description: pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI Plasmid carrying Golden Gate substitution insert for acVHH-assisted bisection mapping
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicillin / Chloramphenicol
owned by: trevorho
date: 09-OCT-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RiboJ
sequence annotation: 2x(GGGGS) linker
sequence annotation: ECK120029600
sequence annotation: L3S2P55
sequence annotation: PBAD(SapI-)
sequence annotation: acVHH
sequence annotation: RFC10 Suffix
sequence annotation: RBS for CDS 1
sequence annotation: VR
sequence annotation: ECK120033737
sequence annotation: cat (BsmBI-)
sequence annotation: rep (pMB1)
sequence annotation: ampR (rev)
sequence annotation: RFC10 Prefix (full)
sequence annotation: RBS for 2nd CDS
sequence annotation: BCD22
sequence annotation: cat promoter
sequence annotation: VF2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180_seq/1

RiboJ
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 2843
end: 2917
orientation: inline

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 3363
end: 3392
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 1529
end: 1618
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 2478
end: 2534
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 2554
end: 2837
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 1161
end: 1517
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 3401
end: 3421
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 2937
end: 2944
orientation: inline

acVHH
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 3006
end: 3362
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 3479
end: 3498
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 1627
end: 1683
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 1712
end: 2371
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 3585
end: 4199
orientation: reverseComplement

2x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: 2x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 1131
end: 1160
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (rev)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 1
end: 861
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 1100
end: 1121
orientation: inline

RBS for 2nd CDS
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: RBS for 2nd CDS
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 2988
end: 2996
orientation: inline

BCD22
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: BCD22
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 2918
end: 3005
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 2372
end: 2469
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180/1
start: 982
end: 1001
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
type: ComponentDefinition
persistent identity: IBMc397
display id: IBMc397
version: 1
description: pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3K3 Origin of replication: p15A Selection Markers: Kanamycin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: L3S3P21
sequence annotation: kanR
sequence annotation: 3x(GGGGS) linker
sequence annotation: VR
sequence annotation: araC
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence annotation: p15A origin of replication
sequence annotation: H6
sequence annotation: L3S2P11
sequence annotation: acVHH
sequence annotation: PBAD(SapI-)
sequence annotation: BBa_B0030
sequence annotation: VF2
sequence annotation: BBa_B0015
sequence annotation: T7 181+
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397_seq/1

L3S3P21
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 1273
end: 1325
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: kanR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 5529
end: 6344
orientation: reverseComplement

3x(GGGGS) linker
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: 3x(GGGGS) linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 2996
end: 3040
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 5447
end: 5466
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 1399
end: 2277
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 5293
end: 5313
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 1251
end: 1272
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
multi range type: join
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation14/range1/1
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation14/range0/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation14/range1/1
type: Range
persistent identity: range1
display id: range1
version: 1
position: position0
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 1
end: 732
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation14/range0/1
type: Range
persistent identity: range0
display id: range0
version: 1
position: position1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 6676
end: 6768
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 2621
end: 2638
orientation: inline

L3S2P11
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: L3S2P11
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 1334
end: 1390
orientation: reverseComplement

acVHH
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: acVHH
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 2639
end: 2995
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 2305
end: 2588
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 2597
end: 2611
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 1133
end: 1152
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0015
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 5164
end: 5292
orientation: inline

T7 181+
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: T7 181+
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397/1
start: 3041
end: 5155
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
type: ComponentDefinition
persistent identity: IBMc419
display id: IBMc419
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(76-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(76-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: M86C
sequence annotation: PhlF
sequence annotation: IOT
sequence annotation: L3S3P21
sequence annotation: ECK120033736
sequence annotation: tetR
sequence annotation: cat (BsmBI-)
sequence annotation: PPhlF
sequence annotation: BBa_B0032
sequence annotation: RFC10 Suffix
sequence annotation: RiboJ
sequence annotation: tetR promoter
sequence annotation: SrpR
sequence annotation: PlacIQ
sequence annotation: VF2
sequence annotation: VR
sequence annotation: L3S2P21
sequence annotation: phl2
sequence annotation: RFC10 Prefix (full)
sequence annotation: cat promoter
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419_seq/1

M86C
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 3391
end: 3552
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 2281
end: 2883
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation1/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2893
end: 3147
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation20/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3148
end: 3200
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation8/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3981
end: 4033
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 98
end: 1291
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 1517
end: 2035
orientation: reverseComplement

PPhlF
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation5/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 3220
end: 3285
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation4/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 3369
end: 3381
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 4034
end: 4054
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
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role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 3286
end: 3360
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 9
end: 97
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 3556
end: 3969
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 2199
end: 2252
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 1371
end: 1390
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 4149
end: 4168
orientation: reverseComplement

L3S2P21
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 2134
end: 2194
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 2253
end: 2280
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 1494
end: 1494
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 2036
end: 2133
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419/1
start: 4615
end: 5439
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
type: ComponentDefinition
persistent identity: IBMc373
display id: IBMc373
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-118)-YCG-ER-LBD-S-M86(119-154)-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-118)-YCG-ER-LBD-S-M86(119-154)-S-ECF20(102-193)-T Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 118/119 by ER-LBD
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033736
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence annotation: M86(1-118)
sequence annotation: araC
sequence annotation: VR
sequence annotation: ECF20 102-193
sequence annotation: VF2
sequence annotation: ECF20 1-101
sequence annotation: M86(119-154)
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Suffix
sequence annotation: BBa_B0033
sequence annotation: ER-LBD
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373_seq/1

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 2422
end: 2705
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 4545
end: 4597
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 98
end: 1291
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 1494
end: 1515
orientation: inline

M86(1-118)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-118)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 3037
end: 3390
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 1516
end: 2394
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 4713
end: 4732
orientation: reverseComplement

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 4258
end: 4533
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 1371
end: 1390
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 2731
end: 3033
orientation: inline

M86(119-154)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(119-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation11/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 4147
end: 4254
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 5179
end: 6003
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 4598
end: 4618
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 2714
end: 2724
orientation: inline

ER-LBD
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ER-LBD
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 3400
end: 4143
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373/1
start: 9
end: 97
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
type: ComponentDefinition
persistent identity: IBMc233
display id: IBMc233
version: 1
description: pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI Plasmid carrying Golden Gate substitution insert for IBM, M86 intien, using PPhlF as C-lobe promoter
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicillin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ECK120033737
sequence annotation: phl2
sequence annotation: M86C
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence annotation: RiboJ
sequence annotation: IOT
sequence annotation: ampR (rev)
sequence annotation: L3S3P21
sequence annotation: PhlF
sequence annotation: BBa_B0032
sequence annotation: ECK120029600
sequence annotation: M86N
sequence annotation: cat promoter
sequence annotation: PPhlF
sequence annotation: PlacIQ
sequence annotation: cat (BsmBI-)
sequence annotation: VR
sequence annotation: L3S2P21
sequence annotation: rep (pMB1)
sequence annotation: VF2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233_seq/1

ECK120033737
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 1543
end: 1599
orientation: reverseComplement

phl2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation10/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 2505
end: 2532
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 3643
end: 3804
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 3816
end: 3836
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 1100
end: 1121
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 3538
end: 3612
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 3145
end: 3399
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR (rev)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 1
end: 861
orientation: reverseComplement

L3S3P21
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 3400
end: 3452
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 2533
end: 3135
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 3621
end: 3633
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 1445
end: 1534
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 1134
end: 1433
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 2288
end: 2385
orientation: reverseComplement

PPhlF
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 3472
end: 3537
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 2451
end: 2504
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 1628
end: 2287
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 3894
end: 3913
orientation: reverseComplement

L3S2P21
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 2386
end: 2446
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 4000
end: 4614
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233/1
start: 982
end: 1001
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
type: ComponentDefinition
persistent identity: IBMc461
display id: IBMc461
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B32-bla(1-28)-BsaI-BsaI-bla(285-286)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B32-bla(1-28)-BsaI-BsaI-bla(285-286)-Ter Expression vector to generate ORF insertion library in IBM on beta-lactamase
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR promoter
sequence annotation: RFC10 Prefix (full)
sequence annotation: BBa_B0032
sequence annotation: VR
sequence annotation: araC
sequence annotation: spacing sequence
sequence annotation: bla
sequence annotation: ECK120033736
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Suffix
sequence annotation: tetR
sequence annotation: PBAD(SapI-)
sequence annotation: VF2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461_seq/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 9
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orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 1494
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orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: BBa_B0032
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 2714
end: 2726
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 3033
end: 3052
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 1516
end: 2394
orientation: reverseComplement

spacing sequence
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: spacing sequence
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 2825
end: 2840
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: bla
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 2848
end: 2853
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: bla
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 2733
end: 2817
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 2865
end: 2917
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 3499
end: 4323
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 2918
end: 2938
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 98
end: 1291
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 2422
end: 2705
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461/1
start: 1371
end: 1390
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
type: ComponentDefinition
persistent identity: IBMc329
display id: IBMc329
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR-Ter Expression plasmid for SrpR
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0030
sequence annotation: RFC10 Prefix (full)
sequence annotation: VF2
sequence annotation: p15A origin of replication
sequence annotation: araC
sequence annotation: ECK120033736
sequence annotation: tetR
sequence annotation: RFC10 Suffix
sequence annotation: tetR promoter
sequence annotation: VR
sequence annotation: PBAD(SapI-)
sequence annotation: SrpR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329_seq/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 2714
end: 2728
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 1494
end: 1515
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 1371
end: 1390
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 4019
end: 4843
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 1516
end: 2394
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 3385
end: 3437
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 98
end: 1291
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 3438
end: 3458
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 9
end: 97
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 3553
end: 3572
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 2422
end: 2705
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329/1
start: 2735
end: 3373
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
type: ComponentDefinition
persistent identity: IBMc101
display id: IBMc101
version: 1
description: pSB3T5(empty)
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(empty) Empty plasmid for generating autofluorescence control strains
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 17-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VF2
sequence annotation: tetR promoter
sequence annotation: tetR
sequence annotation: p15A origin of replication
sequence annotation: VR
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101_seq/1

VF2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
start: 2089
end: 2108
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
start: 727
end: 815
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
start: 816
end: 2009
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
multi range type: join
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation2/range1/1
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation2/range0/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation2/range1/1
type: Range
persistent identity: range1
display id: range1
version: 1
position: position1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
start: 1
end: 387
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation2/range0/1
type: Range
persistent identity: range0
display id: range0
version: 1
position: position0
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
start: 2807
end: 3244
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101/1
start: 2341
end: 2360
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
type: ComponentDefinition
persistent identity: IBMc071
display id: IBMc071
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T ECF20 expression plasmid. Activity reference in IBM experiment on ECF20
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR promoter
sequence annotation: ECF20
sequence annotation: p15A origin of replication
sequence annotation: araC
sequence annotation: RFC10 Prefix (full)
sequence annotation: RFC10 Suffix
sequence annotation: BBa_B0033
sequence annotation: VR
sequence annotation: VF2
sequence annotation: tetR
sequence annotation: PBAD(SapI-)
sequence annotation: ECK120033736
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071_seq/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 9
end: 97
orientation: inline

ECF20
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation7/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 2731
end: 3309
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 3955
end: 4779
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 1516
end: 2394
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 1494
end: 1515
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 3374
end: 3394
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 2714
end: 2724
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 3489
end: 3508
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 1371
end: 1390
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 98
end: 1291
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 2422
end: 2705
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071/1
start: 3321
end: 3373
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
type: ComponentDefinition
persistent identity: IBMc325
display id: IBMc325
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-37R3-2-S-mCherry*(193-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-37R3-2-S-mCherry*(193-236)-H6-Ter Expression plasmid for mCherry split at 192/193 inserted with Mtu RecA 37R3-2
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR
sequence annotation: VF2
sequence annotation: PBAD(SapI-)
sequence annotation: Mtu RecA 37R3-2
sequence annotation: BBa_B0030
sequence annotation: araC
sequence annotation: tetR promoter
sequence annotation: mCherry*(193-236)
sequence annotation: RFC10 Prefix (full)
sequence annotation: p15A origin of replication
sequence annotation: ECK120033736
sequence annotation: VR
sequence annotation: mCherry*(1-192)
sequence annotation: RFC10 Suffix
sequence annotation: H6
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325_seq/1

tetR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 98
end: 1291
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 1371
end: 1390
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 2422
end: 2705
orientation: inline

Mtu RecA 37R3-2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Mtu RecA 37R3-2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 3314
end: 4552
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 2714
end: 2728
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 1516
end: 2394
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 9
end: 97
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 4556
end: 4687
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 1494
end: 1515
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 5351
end: 6175
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 4717
end: 4769
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 4885
end: 4904
orientation: reverseComplement

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 2735
end: 3310
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation7/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 4770
end: 4790
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/annotation11/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325/1
start: 4688
end: 4705
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
type: ComponentDefinition
persistent identity: IBMc372
display id: IBMc372
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-91)-HIG-ER-LBD-S-M86(92-154)-S-ECF20(102-193)-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-91)-HIG-ER-LBD-S-M86(92-154)-S-ECF20(102-193)-T Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 91/92 by ER-LBD
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0033
sequence annotation: ECF20 102-193
sequence annotation: ECF20 1-101
sequence annotation: M86(92-154)
sequence annotation: tetR
sequence annotation: PBAD(SapI-)
sequence annotation: ER-LBD
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECK120033736
sequence annotation: M86(1-91)
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Suffix
sequence annotation: araC
sequence annotation: tetR promoter
sequence annotation: VR
sequence annotation: VF2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372_seq/1

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 2714
end: 2724
orientation: inline

ECF20 102-193
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 102-193
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation0/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 4258
end: 4533
orientation: inline

ECF20 1-101
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20 1-101
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation7/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 2731
end: 3033
orientation: inline

M86(92-154)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(92-154)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 4066
end: 4254
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 98
end: 1291
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 2422
end: 2705
orientation: inline

ER-LBD
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ER-LBD
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 3319
end: 4062
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation2/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 1494
end: 1515
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 4545
end: 4597
orientation: inline

M86(1-91)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86(1-91)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 3037
end: 3309
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 5179
end: 6003
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 4598
end: 4618
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 1516
end: 2394
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 9
end: 97
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 4713
end: 4732
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372/1
start: 1371
end: 1390
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
type: ComponentDefinition
persistent identity: IBMc423
display id: IBMc423
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(115-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(115-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VF2
sequence annotation: RiboJ
sequence annotation: PhlF
sequence annotation: cat (BsmBI-)
sequence annotation: SrpR
sequence annotation: RFC10 Prefix (full)
sequence annotation: IOT
sequence annotation: BBa_B0032
sequence annotation: RFC10 Suffix
sequence annotation: tetR promoter
sequence annotation: VR
sequence annotation: L3S2P21
sequence annotation: L3S3P21
sequence annotation: M86C
sequence annotation: p15A origin of replication
sequence annotation: cat promoter
sequence annotation: phl2
sequence annotation: PPhlF
sequence annotation: tetR
sequence annotation: ECK120033736
sequence annotation: PlacIQ
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423_seq/1

VF2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 1371
end: 1390
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 3286
end: 3360
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 2281
end: 2883
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 1517
end: 2035
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 3556
end: 3852
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 1494
end: 1494
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 2893
end: 3147
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 3369
end: 3381
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 3917
end: 3937
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 9
end: 97
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 4032
end: 4051
orientation: reverseComplement

L3S2P21
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 2134
end: 2194
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 3148
end: 3200
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 3391
end: 3552
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 4498
end: 5322
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 2036
end: 2133
orientation: reverseComplement

phl2
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 2253
end: 2280
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 3220
end: 3285
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 98
end: 1291
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 3864
end: 3916
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423/1
start: 2199
end: 2252
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
type: ComponentDefinition
persistent identity: IBMc468
display id: IBMc468
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-159)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-159)-Ter Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VF2
sequence annotation: rep (pMB1)
sequence annotation: araC
sequence annotation: L3S2P55
sequence annotation: VR
sequence annotation: BBa_B0030
sequence annotation: mCherry*(1-159)
sequence annotation: PBAD(SapI-)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468_seq/1

VF2
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
start: 982
end: 1001
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
start: 3064
end: 3678
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
start: 1118
end: 1996
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
start: 2838
end: 2894
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
start: 2958
end: 2977
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
start: 2316
end: 2330
orientation: inline

mCherry*(1-159)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-159)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
start: 2337
end: 2813
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468/1
start: 2024
end: 2307
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
type: ComponentDefinition
persistent identity: IBMc415
display id: IBMc415
version: 1
description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-175)-QHG-M86N-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-SrpR(1-175)-QHG-M86N-T SrpRN-M86N expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: araC
sequence annotation: pSEVA PS2 Rev
sequence annotation: trfA CDS (replication protein)
sequence annotation: M86N
sequence annotation: BBa_B0030
sequence annotation: ECK120033737
sequence annotation: SrpR
sequence annotation: cat (BsmBI-)
sequence annotation: ECK120029600
sequence annotation: cat
sequence annotation: pSEVA PS1 Fwd
sequence annotation: lambda t0 terminator
sequence annotation: PBAD(SapI-)
sequence annotation: RFC10 Prefix (full)
sequence annotation: cat promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415_seq/1

araC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 78
end: 956
orientation: reverseComplement

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 2619
end: 2635
orientation: reverseComplement

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 3787
end: 4935
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 1831
end: 2130
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 1276
end: 1290
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 2240
end: 2296
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 1297
end: 1821
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 2325
end: 2465
orientation: reverseComplement

ECK120029600
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 2142
end: 2231
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 2810
end: 3469
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 5976
end: 5993
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 2565
end: 2659
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 984
end: 1267
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 57
end: 77
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415/1
start: 2712
end: 2809
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
type: ComponentDefinition
persistent identity: IBMc403
display id: IBMc403
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-194)-PGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(195-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-194)-PGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(195-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: M86C
sequence annotation: BBa_B0030
sequence annotation: cat (BsmBI-)
sequence annotation: VR
sequence annotation: luxR
sequence annotation: VF2
sequence annotation: cat promoter
sequence annotation: p15A origin of replication
sequence annotation: Plux2
sequence annotation: RFC10 Prefix (full)
sequence annotation: H6
sequence annotation: ECK120029600
sequence annotation: tetR
sequence annotation: PBAD(SapI-)
sequence annotation: mCherry*
sequence annotation: M86N
sequence annotation: ECK120033737
sequence annotation: L3S2P55
sequence annotation: BBa_J23117
sequence annotation: ECK120033736
sequence annotation: BBa_B0032
sequence annotation: araC
sequence annotation: tetR promoter
sequence annotation: BBa_B0012
sequence annotation: RFC10 Suffix
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403_seq/1

M86C
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5629
end: 5790
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5438
end: 5452
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 3820
end: 4479
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 6117
end: 6136
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 4676
end: 5431
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 1371
end: 1390
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 4480
end: 4577
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 6583
end: 7407
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5545
end: 5598
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 1494
end: 1515
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5920
end: 5937
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 3637
end: 3726
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 98
end: 1291
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 2422
end: 2705
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 2735
end: 3321
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 3326
end: 3625
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 3735
end: 3791
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 4586
end: 4642
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5794
end: 5919
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5461
end: 5495
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5949
end: 6001
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 2714
end: 2728
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5607
end: 5619
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 1516
end: 2394
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 9
end: 97
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 5496
end: 5536
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403/1
start: 6002
end: 6022
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
type: ComponentDefinition
persistent identity: IBMc326
display id: IBMc326
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter Expression vector to generate ORF insertion library in IBM on ECF20
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR promoter
sequence annotation: ECF20
sequence annotation: tetR
sequence annotation: p15A origin of replication
sequence annotation: BBa_B0033
sequence annotation: araC
sequence annotation: RFC10 Suffix
sequence annotation: PBAD(SapI-)
sequence annotation: VF2
sequence annotation: RFC10 Prefix (full)
sequence annotation: VR
sequence annotation: ECK120033736
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326_seq/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 9
end: 97
orientation: inline

ECF20
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 2731
end: 2747
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 98
end: 1291
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 3452
end: 4276
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: BBa_B0033
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 2714
end: 2724
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 1516
end: 2394
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 2871
end: 2891
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 2422
end: 2705
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 1371
end: 1390
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 1494
end: 1515
orientation: inline

ECF20
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 2785
end: 2809
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 2986
end: 3005
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326/1
start: 2818
end: 2870
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
type: ComponentDefinition
persistent identity: IBMc451
display id: IBMc451
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(186-236)-H6-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(186-236)-H6-Ter Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RBS for CDS2
sequence annotation: SYNZIP18
sequence annotation: VR
sequence annotation: phl2
sequence annotation: ECK120033736
sequence annotation: tetR promoter
sequence annotation: L3S2P21
sequence annotation: RBS for CDS 1
sequence annotation: mCherry*(186-236)
sequence annotation: PlacIQ
sequence annotation: H6
sequence annotation: p15A origin of replication
sequence annotation: IOT
sequence annotation: RiboJ
sequence annotation: RFC10 Prefix (full)
sequence annotation: VF2
sequence annotation: RFC10 Suffix
sequence annotation: BCD1
sequence annotation: tetR
sequence annotation: PhlF
sequence annotation: PPhlF
sequence annotation: L3S3P21
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451_seq/1

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2816
end: 2824
orientation: inline

SYNZIP18
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP18
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2831
end: 2956
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 3322
end: 3341
orientation: reverseComplement

phl2
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 1635
end: 1662
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 3154
end: 3206
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 9
end: 97
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 1516
end: 1576
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2765
end: 2772
orientation: inline

mCherry*(186-236)
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(186-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2972
end: 3124
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation17/range/1

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type: Range
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display id: range
version: 1
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start: 1581
end: 1634
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation16/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3125
end: 3142
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
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role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation10/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 3788
end: 4612
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation11/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2275
end: 2529
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
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role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation4/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2668
end: 2742
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation21/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 1494
end: 1515
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 1371
end: 1390
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 3207
end: 3227
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2746
end: 2830
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 98
end: 1291
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 1663
end: 2265
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2602
end: 2667
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451/1
start: 2530
end: 2582
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
type: ComponentDefinition
persistent identity: IBMc307
display id: IBMc307
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86-S-mCherry*(193-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86-S-mCherry*(193-236)-H6-T Expression plasmid for mCherry split at 192/193 inserted with cis-M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: mCherry*(193-236)
sequence annotation: M86
sequence annotation: VR
sequence annotation: H6
sequence annotation: tetR
sequence annotation: BBa_B0030
sequence annotation: araC
sequence annotation: PBAD(SapI-)
sequence annotation: p15A origin of replication
sequence annotation: mCherry*(1-192)
sequence annotation: VF2
sequence annotation: tetR promoter
sequence annotation: ECK120033736
sequence annotation: RFC10 Prefix (full)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307_seq/1

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 3779
end: 3910
orientation: inline

M86
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 3314
end: 3775
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 4108
end: 4127
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 3911
end: 3928
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 98
end: 1291
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 2714
end: 2728
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 1516
end: 2394
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 2422
end: 2705
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 4574
end: 5398
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 2735
end: 3310
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 1371
end: 1390
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 9
end: 97
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 3940
end: 3992
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307/1
start: 1494
end: 1515
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
type: ComponentDefinition
persistent identity: IBMc426
display id: IBMc426
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(171-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(171-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: PhlF
sequence annotation: PlacIQ
sequence annotation: RFC10 Prefix (full)
sequence annotation: VF2
sequence annotation: p15A origin of replication
sequence annotation: RFC10 Suffix
sequence annotation: M86C
sequence annotation: BBa_B0032
sequence annotation: IOT
sequence annotation: PPhlF
sequence annotation: tetR promoter
sequence annotation: cat promoter
sequence annotation: cat (BsmBI-)
sequence annotation: VR
sequence annotation: ECK120033736
sequence annotation: RiboJ
sequence annotation: L3S3P21
sequence annotation: tetR
sequence annotation: L3S2P21
sequence annotation: SrpR
sequence annotation: phl2
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426_seq/1

PhlF
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
start: 2281
end: 2883
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PlacIQ
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
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role: SO:0000167
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RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #d6b295
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label: RFC10 Prefix (full)
owned by: trevorho
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role: SO:0001411
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start: 1494
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VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
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role: SO:0005850
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p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
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ap einfo fwdcolor: #b4abac
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label: p15A origin of replication
owned by: trevorho
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role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation11/range/1

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type: Range
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start: 4330
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RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation8/range/1

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type: Range
persistent identity: range
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start: 3749
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orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation0/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3391
end: 3552
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation9/range/1

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type: Range
persistent identity: range
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version: 1
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start: 3369
end: 3381
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation5/range/1

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type: Range
persistent identity: range
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start: 2893
end: 3147
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation1/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3220
end: 3285
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation16/range/1

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type: Range
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display id: range
version: 1
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start: 9
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orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation18/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2036
end: 2133
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation7/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 1517
end: 2035
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
start: 3864
end: 3883
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
start: 3696
end: 3748
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
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role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
start: 3286
end: 3360
orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
start: 3148
end: 3200
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
start: 98
end: 1291
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/1
start: 2134
end: 2194
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 3556
end: 3684
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 2253
end: 2280
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
type: ComponentDefinition
persistent identity: IBMc398
display id: IBMc398
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-187)-ANG-M86N-cat-Plux2-B32-M86C-S-mCherry*(188-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-187)-ANG-M86N-cat-Plux2-B32-M86C-S-mCherry*(188-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: luxR
sequence annotation: BBa_B0032
sequence annotation: BBa_B0030
sequence annotation: ECK120029600
sequence annotation: VF2
sequence annotation: mCherry*
sequence annotation: VR
sequence annotation: L3S2P55
sequence annotation: M86N
sequence annotation: ECK120033737
sequence annotation: PBAD(SapI-)
sequence annotation: tetR
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence annotation: tetR promoter
sequence annotation: M86C
sequence annotation: H6
sequence annotation: p15A origin of replication
sequence annotation: araC
sequence annotation: cat (BsmBI-)
sequence annotation: BBa_B0012
sequence annotation: BBa_J23117
sequence annotation: Plux2
sequence annotation: ECK120033736
sequence annotation: cat promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398_seq/1

luxR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation13/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 4655
end: 5410
orientation: reverseComplement

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation0/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 5586
end: 5598
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 2714
end: 2728
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 3616
end: 3705
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 1371
end: 1390
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 2738
end: 3295
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 6117
end: 6136
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 4565
end: 4621
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
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ECK120033737
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation23/range/1

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start: 3714
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orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation2/range/1

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type: Range
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version: 1
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start: 2422
end: 2705
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tetR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 98
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orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation14/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 6002
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orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
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role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation11/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 5417
end: 5431
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation6/range/1

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type: Range
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version: 1
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start: 1494
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orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation10/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 9
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orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation24/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 5608
end: 5769
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation19/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 5920
end: 5937
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation12/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 6583
end: 7407
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 1516
end: 2394
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 3799
end: 4458
orientation: reverseComplement

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 5475
end: 5515
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 5440
end: 5474
orientation: reverseComplement

Plux2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 5524
end: 5577
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 5949
end: 6001
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 4459
end: 4556
orientation: reverseComplement

mCherry*
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398/1
start: 5773
end: 5919
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
type: ComponentDefinition
persistent identity: IBMc389
display id: IBMc389
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-176)-HYG-M86N-cat-Plux2-B32-M86C-S-mCherry*(177-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-176)-HYG-M86N-cat-Plux2-B32-M86C-S-mCherry*(177-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: ECK120033736
sequence annotation: VR
sequence annotation: BBa_J23117
sequence annotation: cat (BsmBI-)
sequence annotation: RFC10 Suffix
sequence annotation: tetR
sequence annotation: BBa_B0012
sequence annotation: ECK120033737
sequence annotation: tetR promoter
sequence annotation: ECK120029600
sequence annotation: Plux2
sequence annotation: BBa_B0030
sequence annotation: L3S2P55
sequence annotation: M86N
sequence annotation: H6
sequence annotation: VF2
sequence annotation: mCherry*
sequence annotation: BBa_B0032
sequence annotation: p15A origin of replication
sequence annotation: cat promoter
sequence annotation: araC
sequence annotation: luxR
sequence annotation: RFC10 Prefix (full)
sequence annotation: PBAD(SapI-)
sequence annotation: M86C
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389_seq/1

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 5949
end: 6001
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 6117
end: 6136
orientation: reverseComplement

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 5407
end: 5441
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 3766
end: 4425
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 6002
end: 6022
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 98
end: 1291
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 5442
end: 5482
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 3681
end: 3737
orientation: reverseComplement

tetR promoter
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 9
end: 97
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 3583
end: 3672
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation24/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 5491
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orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 2714
end: 2728
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 4532
end: 4588
orientation: reverseComplement

M86N
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 3272
end: 3571
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation14/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 5920
end: 5937
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation19/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 1371
end: 1390
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 5740
end: 5919
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 5553
end: 5565
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 2735
end: 3267
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 6583
end: 7407
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 4426
end: 4523
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 1516
end: 2394
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 4622
end: 5377
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 1494
end: 1515
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 5384
end: 5398
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 2422
end: 2705
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389/1
start: 5575
end: 5736
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
type: ComponentDefinition
persistent identity: IBMc388
display id: IBMc388
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-174)-GGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(175-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-174)-GGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(175-236)-H6-T Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: BBa_B0032
sequence annotation: p15A origin of replication
sequence annotation: M86N
sequence annotation: PBAD(SapI-)
sequence annotation: mCherry*
sequence annotation: tetR
sequence annotation: M86C
sequence annotation: ECK120029600
sequence annotation: VF2
sequence annotation: BBa_B0012
sequence annotation: BBa_J23117
sequence annotation: H6
sequence annotation: cat promoter
sequence annotation: RFC10 Suffix
sequence annotation: Plux2
sequence annotation: tetR promoter
sequence annotation: BBa_B0030
sequence annotation: VR
sequence annotation: ECK120033736
sequence annotation: RFC10 Prefix (full)
sequence annotation: araC
sequence annotation: L3S2P55
sequence annotation: cat (BsmBI-)
sequence annotation: luxR
sequence annotation: ECK120033737
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388_seq/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5547
end: 5559
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 6583
end: 7407
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 3266
end: 3565
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 2422
end: 2705
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5734
end: 5919
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 98
end: 1291
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5569
end: 5730
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 3577
end: 3666
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation25/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 1371
end: 1390
orientation: inline

mCherry*
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 2735
end: 3261
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0012
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5436
end: 5476
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: BBa_J23117
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5401
end: 5435
orientation: reverseComplement

H6
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation24/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation24/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5920
end: 5937
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation23/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 4420
end: 4517
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 6002
end: 6022
orientation: inline

Plux2
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: Plux2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5485
end: 5538
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 9
end: 97
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 2714
end: 2728
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 6117
end: 6136
orientation: reverseComplement

ECK120033736
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation22/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation22/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5949
end: 6001
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 1494
end: 1515
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 5378
end: 5392
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation21/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 1516
end: 2394
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 4526
end: 4582
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation26/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 3760
end: 4419
orientation: reverseComplement

luxR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: luxR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 4616
end: 5371
orientation: reverseComplement

ECK120033737
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388/1
start: 3675
end: 3731
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
type: ComponentDefinition
persistent identity: IBMc394
display id: IBMc394
version: 1
description: pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-14aa-FRB-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-14aa-FRB-T Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to FRB
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA321 Origin of replication: RK2 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: FRB E coli optimized
sequence annotation: trfA CDS (replication protein)
sequence annotation: 29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T
sequence annotation: ECK120033736
sequence annotation: BBa_B0030
sequence annotation: cat promoter
sequence annotation: PBAD(SapI-)
sequence annotation: RFC10 Suffix
sequence annotation: H6
sequence annotation: pSEVA PS2 Rev
sequence annotation: araC
sequence annotation: Long Linker
sequence annotation: cat
sequence annotation: lambda t0 terminator
sequence annotation: pSEVA PS1 Fwd
sequence annotation: RFC10 Prefix (full)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394_seq/1

FRB E coli optimized
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: FRB E coli optimized
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 1894
end: 2172
orientation: inline

trfA CDS (replication protein)
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: trfA CDS (replication protein)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 3534
end: 4682
orientation: reverseComplement

29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: 29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 1318
end: 1851
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 2184
end: 2236
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 1276
end: 1290
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 2459
end: 2556
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 984
end: 1267
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 2237
end: 2257
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 1300
end: 1317
orientation: inline

pSEVA PS2 Rev
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS2 Rev
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 2366
end: 2382
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 78
end: 956
orientation: reverseComplement

Long Linker
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: Long Linker
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 1852
end: 1893
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 2557
end: 3216
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 2312
end: 2406
orientation: inline

pSEVA PS1 Fwd
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: pSEVA PS1 Fwd
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
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top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 5723
end: 5740
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394/1
start: 56
end: 77
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
type: ComponentDefinition
persistent identity: IBMc308
display id: IBMc308
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-mCherry*(193-236)-H6-T
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-mCherry*(193-236)-H6-T Expression vector to generate ORF insertion library in ER-LBD domain-insertion mapping
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: RFC10 Suffix
sequence annotation: VR
sequence annotation: tetR
sequence annotation: VF2
sequence annotation: tetR promoter
sequence annotation: H6
sequence annotation: ECK120033736
sequence annotation: mCherry*(1-192)
sequence annotation: RFC10 Prefix (full)
sequence annotation: BBa_B0030
sequence annotation: PBAD(SapI-)
sequence annotation: araC
sequence annotation: M86C
sequence annotation: p15A origin of replication
sequence annotation: M86N
sequence annotation: spacing sequence
sequence annotation: mCherry*(193-236)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308_seq/1

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation6/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 3599
end: 3619
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
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role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation7/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 3714
end: 3733
orientation: reverseComplement

tetR
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 98
end: 1291
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 1371
end: 1390
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 9
end: 97
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 3517
end: 3534
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 3546
end: 3598
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 2735
end: 3310
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 1494
end: 1515
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 2714
end: 2728
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 2422
end: 2705
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 1516
end: 2394
orientation: reverseComplement

M86C
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 3363
end: 3381
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 4180
end: 5004
orientation: inline

M86N
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86N
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 3314
end: 3332
orientation: inline

spacing sequence
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: spacing sequence
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 3340
end: 3355
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308/1
start: 3385
end: 3516
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
type: ComponentDefinition
persistent identity: IBMc367
display id: IBMc367
version: 1
description: pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3 Staging plasmid for IBM on ECF20
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1C3 Origin of replication: pMB1 Selection Markers: Chloramphenicol
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VF2
sequence annotation: VR
sequence annotation: rep (pMB1)
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence annotation: ECF20
sequence annotation: lambda t0 terminator
sequence annotation: cat (BsmBI-)
sequence annotation: cat promoter
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367_seq/1

VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
start: 992
end: 1011
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
start: 1848
end: 1867
orientation: reverseComplement

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
start: 1954
end: 2568
orientation: reverseComplement

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
start: 1694
end: 1714
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
start: 1110
end: 1131
orientation: inline

ECF20
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: ECF20
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
start: 1139
end: 1685
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: lambda t0 terminator
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
start: 106
end: 200
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
start: 221
end: 880
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
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start: 881
end: 978
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
type: ComponentDefinition
persistent identity: IBMc424
display id: IBMc424
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(117-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(117-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: L3S3P21
sequence annotation: cat promoter
sequence annotation: SrpR
sequence annotation: p15A origin of replication
sequence annotation: VR
sequence annotation: IOT
sequence annotation: PPhlF
sequence annotation: tetR promoter
sequence annotation: PhlF
sequence annotation: RFC10 Suffix
sequence annotation: PlacIQ
sequence annotation: L3S2P21
sequence annotation: phl2
sequence annotation: RiboJ
sequence annotation: cat (BsmBI-)
sequence annotation: BBa_B0032
sequence annotation: ECK120033736
sequence annotation: VF2
sequence annotation: tetR
sequence annotation: RFC10 Prefix (full)
sequence annotation: M86C
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424_seq/1

L3S3P21
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S3P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation14/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 3148
end: 3200
orientation: inline

cat promoter
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation17/range/1

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type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 2036
end: 2133
orientation: reverseComplement

SrpR
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 3556
end: 3846
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 4492
end: 5316
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 4026
end: 4045
orientation: reverseComplement

IOT
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 2893
end: 3147
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 3220
end: 3285
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 9
end: 97
orientation: inline

PhlF
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 2281
end: 2883
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 3911
end: 3931
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 2199
end: 2252
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 2134
end: 2194
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 2253
end: 2280
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 3286
end: 3360
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 1517
end: 2035
orientation: reverseComplement

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 3369
end: 3381
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 3858
end: 3910
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 1371
end: 1390
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 98
end: 1291
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 1494
end: 1494
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation15/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/annotation15/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424/1
start: 3391
end: 3552
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
type: ComponentDefinition
persistent identity: IBMc428
display id: IBMc428
version: 1
description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(176-213)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(176-213)-Ter M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat promoter
sequence annotation: tetR
sequence annotation: SrpR
sequence annotation: VF2
sequence annotation: BBa_B0032
sequence annotation: ECK120033736
sequence annotation: L3S2P21
sequence annotation: L3S3P21
sequence annotation: RFC10 Suffix
sequence annotation: IOT
sequence annotation: cat (BsmBI-)
sequence annotation: PhlF
sequence annotation: PlacIQ
sequence annotation: VR
sequence annotation: RFC10 Prefix (full)
sequence annotation: PPhlF
sequence annotation: M86C
sequence annotation: RiboJ
sequence annotation: tetR promoter
sequence annotation: phl2
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428_seq/1

cat promoter
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation13/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 2036
end: 2133
orientation: reverseComplement

tetR
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 98
end: 1291
orientation: inline

SrpR
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: SrpR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation18/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation18/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 3556
end: 3669
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 1371
end: 1390
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: BBa_B0032
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation20/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 3369
end: 3381
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation12/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 3681
end: 3733
orientation: inline

L3S2P21
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: L3S2P21
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation8/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 2134
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orientation: inline

L3S3P21
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #84b0dc
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label: L3S3P21
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation15/range/1

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type: Range
persistent identity: range
display id: range
version: 1
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start: 3148
end: 3200
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation14/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation14/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 3734
end: 3754
orientation: inline

IOT
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: IOT
owned by: trevorho
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role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 2893
end: 3147
orientation: inline

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation17/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 1517
end: 2035
orientation: reverseComplement

PhlF
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: PhlF
owned by: trevorho
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role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
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start: 2281
end: 2883
orientation: inline

PlacIQ
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: PlacIQ
owned by: trevorho
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role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 2199
end: 2252
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 3849
end: 3868
orientation: reverseComplement

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
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role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 1494
end: 1494
orientation: inline

PPhlF
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: PPhlF
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation10/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 3220
end: 3285
orientation: inline

M86C
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86C
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 3391
end: 3552
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
feature type: insulator
label: RiboJ
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000627
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 3286
end: 3360
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation19/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 9
end: 97
orientation: inline

phl2
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: phl2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation11/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 2253
end: 2280
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation16/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428/1
start: 4315
end: 5139
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
type: ComponentDefinition
persistent identity: IBMc482
display id: IBMc482
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-191)-5aa-SYNZIP17-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-191)-5aa-SYNZIP17-Ter Expression plasmid for mCherry N-lobe-SYNZIP17. Used in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: mCherry*(1-191)
sequence annotation: rep (pMB1)
sequence annotation: VR
sequence annotation: PBAD(SapI-)
sequence annotation: L3S2P55
sequence annotation: araC
sequence annotation: ampR
sequence annotation: VF2
sequence annotation: BBa_B0030
sequence annotation: SYNZIP17
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482_seq/1

mCherry*(1-191)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-191)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 2337
end: 2909
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 3301
end: 3915
orientation: reverseComplement

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 3195
end: 3214
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation9/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 2024
end: 2307
orientation: inline

L3S2P55
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 3075
end: 3131
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 1118
end: 1996
orientation: reverseComplement

ampR
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 1
end: 861
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 982
end: 1001
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 2316
end: 2330
orientation: inline

SYNZIP17
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: SYNZIP17
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482/1
start: 2925
end: 3050
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
type: ComponentDefinition
persistent identity: IBMc472
display id: IBMc472
version: 1
description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-176)-Ter
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1A3-PBAD(SapI-)-B30-mCherry*(1-176)-Ter Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicllin
owned by: trevorho
date: 18-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: VF2
sequence annotation: ampR
sequence annotation: L3S2P55
sequence annotation: rep (pMB1)
sequence annotation: PBAD(SapI-)
sequence annotation: mCherry*(1-176)
sequence annotation: VR
sequence annotation: araC
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472_seq/1

VF2
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 982
end: 1001
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: ampR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation0/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 1
end: 861
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0000141
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 2889
end: 2945
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 3115
end: 3729
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0000167
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation8/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 2024
end: 2307
orientation: inline

mCherry*(1-176)
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-176)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 2340
end: 2864
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation7/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 3009
end: 3028
orientation: reverseComplement

araC
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 1118
end: 1996
orientation: reverseComplement

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
role: SO:0000139
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472/1
start: 2316
end: 2330
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
type: ComponentDefinition
persistent identity: IBMc181
display id: IBMc181
version: 1
description: pSB1C3(BsmBI-,BsaI+)-BsaI-M86(6-149)-BsaI
creator: Baojun Wang
creator: Trevor Y. H. Ho
mutable description: pSB1C3(BsmBI-,BsaI+)-BsaI-M86(6-149)-BsaI Staging plasmid for insertion on the M86 intein
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1C3 Origin of replication: pMB1 Selection Markers: Chloramphenicol
owned by: trevorho
date: 17-NOV-2020
molecule: ds-DNA
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: cat (BsmBI-)
sequence annotation: M86
sequence annotation: VR
sequence annotation: VF2
sequence annotation: RFC10 Suffix
sequence annotation: RFC10 Prefix (full)
sequence annotation: rep (pMB1)
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181_seq/1

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
multi range type: join
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation0/range1/1
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation0/range0/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation0/range1/1
type: Range
persistent identity: range1
display id: range1
version: 1
position: position0
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
start: 1
end: 343
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation0/range0/1
type: Range
persistent identity: range0
display id: range0
version: 1
position: position1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
start: 2201
end: 2517
orientation: reverseComplement

M86
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #84b0dc
ap einfo revcolor: #84b0dc
label: M86
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
role: SO:0000316
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation1/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
start: 602
end: 1033
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation6/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
start: 1195
end: 1214
orientation: reverseComplement

VF2
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
role: SO:0005850
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation3/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
start: 455
end: 474
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation5/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
start: 1041
end: 1061
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
role: SO:0001411
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation4/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
start: 573
end: 594
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: rep (pMB1)
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
role: SO:0000296
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation2/range/1

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
owned by: trevorho
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181/1
start: 1301
end: 1915
orientation: reverseComplement

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc050_seq/1
type: Sequence
persistent identity: IBMc050 seq
display id: IBMc050_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGAAGACGCTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc450_seq/1
type: Sequence
persistent identity: IBMc450 seq
display id: IBMc450_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc252_seq/1
type: Sequence
persistent identity: IBMc252 seq
display id: IBMc252_seq
version: 1
owned by: trevorho
elements: aacgcagcggtggtaacggcgcagtggcggttttcatggcttgttatgactgtttttttggggtacagtctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAGACaaTTGTCCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATT…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc419_seq/1
type: Sequence
persistent identity: IBMc419 seq
display id: IBMc419_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc052_seq/1
type: Sequence
persistent identity: IBMc052 seq
display id: IBMc052_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc323_seq/1
type: Sequence
persistent identity: IBMc323 seq
display id: IBMc323_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc423_seq/1
type: Sequence
persistent identity: IBMc423 seq
display id: IBMc423_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc472_seq/1
type: Sequence
persistent identity: IBMc472 seq
display id: IBMc472_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc418_seq/1
type: Sequence
persistent identity: IBMc418 seq
display id: IBMc418_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc429_seq/1
type: Sequence
persistent identity: IBMc429 seq
display id: IBMc429_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc392_seq/1
type: Sequence
persistent identity: IBMc392 seq
display id: IBMc392_seq
version: 1
owned by: trevorho
elements: tatactggcttactatgttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttcccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaaggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattccatggtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagCCAATTATTGAAGGCCTCCCTAACGGGGGGCCTTTTTTTGTTTCTGGTCTCCCTACTAGAGGGACCAAAACGAAAAAAGACGCTCGAAAGCGTCTCTTTTCTGGAATTTGGTACCGAGTACTAGAGTTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCG…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc417_seq/1
type: Sequence
persistent identity: IBMc417 seq
display id: IBMc417_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc471_seq/1
type: Sequence
persistent identity: IBMc471 seq
display id: IBMc471_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334_seq/1
type: Sequence
persistent identity: IBMc334 seq
display id: IBMc334_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc188_seq/1
type: Sequence
persistent identity: IBMc188 seq
display id: IBMc188_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc448_seq/1
type: Sequence
persistent identity: IBMc448 seq
display id: IBMc448_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc337_seq/1
type: Sequence
persistent identity: IBMc337 seq
display id: IBMc337_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc175_seq/1
type: Sequence
persistent identity: IBMc175 seq
display id: IBMc175_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc186_seq/1
type: Sequence
persistent identity: IBMc186 seq
display id: IBMc186_seq
version: 1
owned by: trevorho
elements: ctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatctcgaggttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttgg…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc453_seq/1
type: Sequence
persistent identity: IBMc453 seq
display id: IBMc453_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc385_seq/1
type: Sequence
persistent identity: IBMc385 seq
display id: IBMc385_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc407_seq/1
type: Sequence
persistent identity: IBMc407 seq
display id: IBMc407_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgttt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc369_seq/1
type: Sequence
persistent identity: IBMc369 seq
display id: IBMc369_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc390_seq/1
type: Sequence
persistent identity: IBMc390 seq
display id: IBMc390_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc386_seq/1
type: Sequence
persistent identity: IBMc386 seq
display id: IBMc386_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc368_seq/1
type: Sequence
persistent identity: IBMc368 seq
display id: IBMc368_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc228_seq/1
type: Sequence
persistent identity: IBMc228 seq
display id: IBMc228_seq
version: 1
owned by: trevorho
elements: ttacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaAACgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGGTCTCAggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcgTGAGACCtactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc399_seq/1
type: Sequence
persistent identity: IBMc399 seq
display id: IBMc399_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc451_seq/1
type: Sequence
persistent identity: IBMc451 seq
display id: IBMc451_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc406_seq/1
type: Sequence
persistent identity: IBMc406 seq
display id: IBMc406_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACT…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc475_seq/1
type: Sequence
persistent identity: IBMc475 seq
display id: IBMc475_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc391_seq/1
type: Sequence
persistent identity: IBMc391 seq
display id: IBMc391_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttgcggccgcgtcgtgactgggaaaaccctggcgactagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaggggtccccaataattacgatttaaattggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttgacttttgtccttttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgtaggccggccgcgatgcaggtggctgctgaacccccagccggaactgaccccacaaggccctagcgtttgcaatgcaccaggtcatcattgacccaggcgtgttccaccaggccgctgcctcgcaactcttcgcaggcttcgccgacctgctcgcgccacttcttcacgcgggtggaatccgatccgcacatgaggcggaaggtttccagcttgagcgggtacggctcccggtgcgagctgaaatagtcgaacatccgtcgggccgtcggcgacagcttgcggtacttctcccatatgaatttcgtgtagtggtcgccagcaaacagcacgacgatttcctcgtcgatcaggacctggcaacgggacgttttcttgccacggtccaggacgcggaagcggtgcagcagcgacaccgattccaggtgcccaacgcggtcggacgtgaagcccatcgccgtcgcctgtaggcgcgacaggcattcctcggccttcgtgtaataccggccattgatcgaccagcccaggtcctggcaaagctcgtagaacgtgaaggtgatcggctcgccgataggggtgcgcttcgcgtactccaacacctgctgccacaccagttcgtcatcgtcggcccgcagctcgacgccggtgtaggtgatcttcacgtccttgttgacgtggaaaatgac…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc180_seq/1
type: Sequence
persistent identity: IBMc180 seq
display id: IBMc180_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGAAGACGCTGGAGGCGGTGGTAGCGGTGGTGGCGGTAGCGAAGTTCAGCTGCAAGCAAGCGGTGGTGGTCTGGTTCAGGCAGGCGGTAGCCTGCGTCTGAGCTGTACCGCAAGCGGTCGTACCGGCACCATTTATAGCATGGCATGGTTTCGTCAGGCACCGGGTAAAGAACGTGAATTTCTGGCAACCGTTGGTTGGAGCAGCGGTATTACCTATTATATGGATAGCGTGAAAGGTCGCTTTACCATTAGCCGTGATAATGCAAAAAATAGCGCCTACCTGCAAATGAATAGCCTGAAACCGGAAGATACCGCAGTGTATTATTGTACAGCAACCCGTGCATATAGCGTGGGTTATGATTATTGGGGTCAGGGCACCCAGGTTACCGTTAGCCATtaaTACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCC…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc470_seq/1
type: Sequence
persistent identity: IBMc470 seq
display id: IBMc470_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc457_seq/1
type: Sequence
persistent identity: IBMc457 seq
display id: IBMc457_seq
version: 1
owned by: trevorho
elements: gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaacc…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc326_seq/1
type: Sequence
persistent identity: IBMc326 seq
display id: IBMc326_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc456_seq/1
type: Sequence
persistent identity: IBMc456 seq
display id: IBMc456_seq
version: 1
owned by: trevorho
elements: gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaacc…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc097_seq/1
type: Sequence
persistent identity: IBMc097 seq
display id: IBMc097_seq
version: 1
owned by: trevorho
elements: tggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaAACgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGGTCTCAgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagAAAaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagTGAGACCtactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaag…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc478_seq/1
type: Sequence
persistent identity: IBMc478 seq
display id: IBMc478_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc394_seq/1
type: Sequence
persistent identity: IBMc394 seq
display id: IBMc394_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaaTACTAGATGcatcatcaccatcaccacaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtTCAgctcgcGGAcagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattGCCcgcatcaacgactggtttgaggaagtgaaagctaagcgcggcAGGcgcccgacagccttccagttcCTAAAAgaaatcaagccggaagccgtagcgtacatcaccattaagaccTCTctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcggACCattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagGGTGGCTCTGGCTCGAGCGGTGGCTCTGGCTCTGGCTCGAGCGCCGTGTTATGGCACGAACAGTGGTATGAGGGTTTGGATGACGCATCGCGCCAGTTCTTCGGAGAACATAACACCGAAAAAATGTTTGCGGCACTGGAACCTCTTTA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc414_seq/1
type: Sequence
persistent identity: IBMc414 seq
display id: IBMc414_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGAAAGCGGTCTGATTCATAATCGTATGGTTCAGGCAAGCGATCGTTTTCTGCAATATATTCATCAGGTTCTGCGTCATGCAGTTACCCAGGGTGAACTGCCGATTAATCTGGATCTGCAAACCAGCATTGGTGTTTTTAAAGGTCTGATTACCGGTCTGCTGTATGAAGGTCTGCGTAGTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaac…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc249_seq/1
type: Sequence
persistent identity: IBMc249 seq
display id: IBMc249_seq
version: 1
owned by: trevorho
elements: gggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtttactagaggtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcag…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc473_seq/1
type: Sequence
persistent identity: IBMc473 seq
display id: IBMc473_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc413_seq/1
type: Sequence
persistent identity: IBMc413 seq
display id: IBMc413_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGAAAGCGGTCTGATTCATAATCGTATGGTTCAGGCAAGCGATCGTTTTCTGCAATATATTCATCAGGTTCTGCGTCATGCAGTTACCCAGGGTGAACTGCCGATTAATCTGGATCTGCAAACCAGCATTGGTGTTTTTAAAGGTCTGATTACCGGTCTGCTGTATGAAGGTCTGCGTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcg…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc480_seq/1
type: Sequence
persistent identity: IBMc480 seq
display id: IBMc480_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc424_seq/1
type: Sequence
persistent identity: IBMc424 seq
display id: IBMc424_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc460_seq/1
type: Sequence
persistent identity: IBMc460 seq
display id: IBMc460_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGAAGACGCTGGATATTGTCTGGATCTGAAAACCCAGGTTCAGACACCGCAGGGTATGAAAGAAATTTCAAATATTCAGGTGGGTGATCTGGTTCTGAGCAATACCGGTTATAATGAAGTGCTGAATGTGTTTCCGAAAAGCAAAAAAAAAAGCTATAAAATCACCCTGGAAGATGGCAAAGAAATCATTTGTAGCGAAGAACACCTGTTTCCGACCCAGACCGGTGAAATGAATATTAGCGGTGGTCTGAAAGAAGGTATGTGCCTGTATGTTAAAGAATAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCC…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc397_seq/1
type: Sequence
persistent identity: IBMc397 seq
display id: IBMc397_seq
version: 1
owned by: trevorho
elements: tcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttcccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaaggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattccatggtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagCCAATTATTGAAGGCCTCCCTAACGGGGGGCCTTTTTTTGTTTCTGGTCTCCCTACTAGAGGGACCAAAACGAAAAAAGACGCTCGAAAGCGTCTCTTTTCTGGAATTTGGTACCGAGTACTAGAGTTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGT…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc393_seq/1
type: Sequence
persistent identity: IBMc393 seq
display id: IBMc393_seq
version: 1
owned by: trevorho
elements: ctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc330_seq/1
type: Sequence
persistent identity: IBMc330 seq
display id: IBMc330_seq
version: 1
owned by: trevorho
elements: ATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAAGCGGATAACAATTTCACACAGGAGGCCGCCTAGGCCGCGGCCGCGCGAATTCcttctagagGAAGACagtacgagaggattcgttaccaattgacagctagctcagtcctaggtATATACATACATGCTTGTTTGTTTGTAAACTACTAGAGATTAAAGAGGAGAAATACTAGatggtgagtaaaggagaagctgtgattaaagagttcatgcgcttcaaagttcacatggagggttctatgaacggtcacgagttcgagatcgaaggcgaaggcgagggccgtccgtatgaaggcacccagaccgccaaactgaaagtgactaaaggcggcccgctgcctttttcctgggacatcctgagcccgcaatttatgtacggttctagggcgttcatcaaacacccagcggatatcccggactattataagcagtcttttccggaaggtttcaagtgggaacgcgtaatgaattttgaagatggtggtgccgtgaccgtcactcaggacacctccctggaggatggcaccctgatctataaagttaaactgcgtggtactaattttccacctgatggcccggtgatgcagaaaaagacgatgggttgggaggcgtctaccgaacgcttgtatccggaagatggtgtgctgaaaggcgacattaaaatggccctgcgcctgaaagatggcggccgctatctggctgacttcaaaaccacgtacaaagccaagaaacctgtgcagatgcctggcgcgtacaatgtggaccgcaaactggacatcacctctcataatgaagattatacggtggtagagcaatatgagcgctccgagggtcgtcattctaccggtggcatggatgaactatacaaataataaCTCGGTACCAAATTCCAGAAAAGAGACGCTTTCGAGCGTCTTTTTTCGTTTTGGTCCgtacagctgcGTCTTCtactagtaCTGCAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTaaattagtagcccgcctaatgagcgggcttttttttaattcccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagcattcagcattttcgtgtggcgctgattccgttttttgcggcgttttgcctgccggtgtttgcgcatccggaaaccctggtgaaagtgaaagatgcggaagatcaactgggtgcgcgcgtgggctatattgaactggatctgaacagcggcaaaattctggaatcttttcgtccggaagaacgttttccgatgatgagcacctttaaagtgctgctgtgcggtgcggttctgagccgtgtggatgcgggccaggaacaactgggccgtcgtattcattatagccagaacgatctggtggaatatagcccggtgaccgaaaaacatctgaccgatggcatgaccgtgcgtgaactgtgcagcgcggcgattaccatgagcgataacaccgcggcgaacctgctgctgacgaccattggcggtccgaaagaactgaccgcgtttctgcataacatgggcgatcatgtgacccgtctggatcgttgggaaccggaactgaacgaagcgattccgaacgatgaac…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc189_seq/1
type: Sequence
persistent identity: IBMc189 seq
display id: IBMc189_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc181_seq/1
type: Sequence
persistent identity: IBMc181 seq
display id: IBMc181_seq
version: 1
owned by: trevorho
elements: gccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGGTCTCAagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaagctcctctttacaattggcaccagaaatagaaaagttgcctcagagtgatatttactgggaccccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggactacgtaactttgtcgccaatgacTGAGACCtactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc427_seq/1
type: Sequence
persistent identity: IBMc427 seq
display id: IBMc427_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc367_seq/1
type: Sequence
persistent identity: IBMc367 seq
display id: IBMc367_seq
version: 1
owned by: trevorho
elements: ttatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctcgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaAACgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGGTCTCAatcctgatctggaactgctgaaacgtattggtaataatgatgcacaggccgttaaagaaatggttacccgtaaactgcctcgtctgctggcactggcaagtcgcctgctgggtgatgcagatgaagcacgtgatattgcacaagaaagttttctgcgcatttggaaacaggcagcaagctggcgtagcgaacaggcacgttttgatacctggctgcatcgtgttgcactgaatctgtgttatgatcgtctgcgtcgtcgtaaagaacatgtgccggttgatagcgaacatgcctgtgaagcactggatacccgtccggcaccggatgaacagctggaagcaagcgcacagagccgtcgtatggcacaggcactggatcagctgccggatcgtcagcgtgaagcaattgttctgcagtattatcaagaactgagcaataccgaagcagcagcactgatgcaaattagcgttgaagccctggaaagcctgctgagccgtgcacgtcgtaatctgcgtagccatctggccgaagcaccgggtgcagatctgTGAGACCtactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcac…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc396_seq/1
type: Sequence
persistent identity: IBMc396 seq
display id: IBMc396_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaaTACTAGATGcatcatcaccatcaccacaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtTCAgctcgcGGAcagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattGCCcgcatcaacgactggtttgaggaagtgaaagctaagcgcggcAGGcgcccgacagccttccagttcCTAAAAgaaatcaagccggaagccgtagcgtacatcaccattaagaccTCTctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcggACCattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagGGTGGTGGCGGTAGCGGAGGCGGTGGTAGCGGTGGTGGCGGTAGCGAAGTTCAGCTGCAAGCAAGCGGTGGTGGTCTGGTTCAGGCAGGCGGTAGCCTGCGTCTGAGCTGTACCGCAAGCGGTCGTACCGGCACCATTTATAGCATGGC…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc446_seq/1
type: Sequence
persistent identity: IBMc446 seq
display id: IBMc446_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc329_seq/1
type: Sequence
persistent identity: IBMc329 seq
display id: IBMc329_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc374_seq/1
type: Sequence
persistent identity: IBMc374 seq
display id: IBMc374_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc412_seq/1
type: Sequence
persistent identity: IBMc412 seq
display id: IBMc412_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGAAAGCGGTCTGATTCATAATCGTATGGTTCAGGCAAGCGATCGTTTTCTGCAATATATTCATCAGGTTCTGCGTCATGCAGTTACCCAGGGTGAACTGCCGATTAATCTGGATCTGCAAACCAGCATTGGTGTTTTTAAAGGTCTGATTACCGGTCTGCTGTATGAAGGTCTTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgact…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc191_seq/1
type: Sequence
persistent identity: IBMc191 seq
display id: IBMc191_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc402_seq/1
type: Sequence
persistent identity: IBMc402 seq
display id: IBMc402_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc469_seq/1
type: Sequence
persistent identity: IBMc469 seq
display id: IBMc469_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc370_seq/1
type: Sequence
persistent identity: IBMc370 seq
display id: IBMc370_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc389_seq/1
type: Sequence
persistent identity: IBMc389 seq
display id: IBMc389_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc428_seq/1
type: Sequence
persistent identity: IBMc428 seq
display id: IBMc428_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc477_seq/1
type: Sequence
persistent identity: IBMc477 seq
display id: IBMc477_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc090_seq/1
type: Sequence
persistent identity: IBMc090 seq
display id: IBMc090_seq
version: 1
owned by: trevorho
elements: ccaagcctcgagctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcctgcataacgcgaaGATCTGGAGCGtCttcCGAAAAACGCGAAAGCGTTTCACGATAAATGCGAAAACTGTCTGGATCTGAATAATTAATTAAATTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGGTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTGTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGGAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTACTAGAGttgagaagagaaaagaaaaccgccgat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc372_seq/1
type: Sequence
persistent identity: IBMc372 seq
display id: IBMc372_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc382_seq/1
type: Sequence
persistent identity: IBMc382 seq
display id: IBMc382_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc333_seq/1
type: Sequence
persistent identity: IBMc333 seq
display id: IBMc333_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc452_seq/1
type: Sequence
persistent identity: IBMc452 seq
display id: IBMc452_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc403_seq/1
type: Sequence
persistent identity: IBMc403 seq
display id: IBMc403_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc476_seq/1
type: Sequence
persistent identity: IBMc476 seq
display id: IBMc476_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc401_seq/1
type: Sequence
persistent identity: IBMc401 seq
display id: IBMc401_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc307_seq/1
type: Sequence
persistent identity: IBMc307 seq
display id: IBMc307_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc371_seq/1
type: Sequence
persistent identity: IBMc371 seq
display id: IBMc371_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc388_seq/1
type: Sequence
persistent identity: IBMc388 seq
display id: IBMc388_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc318_seq/1
type: Sequence
persistent identity: IBMc318 seq
display id: IBMc318_seq
version: 1
owned by: trevorho
elements: taaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGAAGACGCTGGAAATAGCCTGGCACTGAGCCTGACCGCAGATCAGATGGTTAGCGCACTGCTGGATGCAGAACCGCCTATTCTGTATAGCGAATATGATCCGACACGTCCGTTTAGCGAAGCAAGCATGATGGGTCTGCTGACCAATCTGGCAGATCGTGAACTGGTTCATATGATTAATTGGGCAAAACGTGTTCCGGGTTTTGTTGATCTGACCCTGCATGATCAGGTTCATCTGCTGGAATGTGCATGGCTGGAAATTCTGATGATTGGTCTGGTTTGGCGTAGCATGGAACATCCGGGTAAACTGCTGTTTGCACCGAATCTGCTGCTGGATCGTAATCAGGGTAAATGTGTTGAAGGTATGGTGGAAATCTTTGATATGCTGCTGGCAACCAGCAGCCGTTTTCGTATGATGAATCTGCAAGGTGAAGAATTTGTTTGCCTGAAAAGCATTATTCTGCTGAACAGCGGTGTTTATACCTTTCTGAGCAGCACCCTGAAAAGTCTGGAAGAAAAAGATCATATCCATCGTGTGCTGGATAAAATCACCGATACACTGATTCATCTGATGGCAAAAGCCGGTCTGACCTTACAGCAGCAGCATCAGCGTCTGGCACAACTGCTGCTGATTCTGAGCCATATTCGTCATATGAGCAATAAAGGTATGGAACACCTGTACAGCATGAAATGCAAAAATGTTGTTCCGCTGTATGACCTGCTGTTAGAAATGCTGGACGCACATCGTCTGCATGCATCAAGAAGAGCtactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtc…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc461_seq/1
type: Sequence
persistent identity: IBMc461 seq
display id: IBMc461_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc426_seq/1
type: Sequence
persistent identity: IBMc426 seq
display id: IBMc426_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc459_seq/1
type: Sequence
persistent identity: IBMc459 seq
display id: IBMc459_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc339_seq/1
type: Sequence
persistent identity: IBMc339 seq
display id: IBMc339_seq
version: 1
owned by: trevorho
elements: GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTaaattagtagcccgcctaatgagcgggcttttttttaattcccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagcattcagcattttcgtgtggcgctgattccgttttttgcggcgttttgcctgccggtgtttgcgcatccggaaaccctggtgaaagtgaaagatgcggaagatcaactgggtgcgcgcgtgggctatattgaactggatctgaacagcggcaaaattctggaatcttttcgtccggaagaacgttttccgatgatgagcacctttaaagtgctgctgtgcggtgcggttctgagccgtgtggatgcgggccaggaacaactgggccgtcgtattcattatagccagaacgatctggtggaatatagcccggtgaccgaaaaacatctgaccgatggcatgaccgtgcgtgaactgtgcagcgcggcgattaccatgagcgataacaccgcggcgaacctgctgctgacgaccattggcggtccgaaagaactgaccgcgtttctgcataacatgggcgatcatgtgacccgtctggatcgttgggaaccggaactgaacgaagcgattccgaacgatgaacgtgataccaccatgccggcagcaatggcgaccaccctgcgtaaactgctgacgggtgagctgctgaccctggcaagccgccagcaactgattgattggatggaagcggataaagtggcgggtccgctgctgcgtagcgcgctgccggctggctggtttattgcggataaaagcggtgcgggcgaacgtggcagccgtggcattattgcggcgctgggcccggatggtaaaccgagccgtattgtggtgatttataccaccggcagccaggcgacgatggatgaacgtaaccgtcagattgcggaaattggcgcgagcctgattaaacattggtaaaccgatacaattaaaggctccttttggagcctttttttttggacgacccTTGTCCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATT…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc233_seq/1
type: Sequence
persistent identity: IBMc233 seq
display id: IBMc233_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGAAGACGCTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc379_seq/1
type: Sequence
persistent identity: IBMc379 seq
display id: IBMc379_seq
version: 1
owned by: trevorho
elements: gagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaa…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc449_seq/1
type: Sequence
persistent identity: IBMc449 seq
display id: IBMc449_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc482_seq/1
type: Sequence
persistent identity: IBMc482 seq
display id: IBMc482_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc384_seq/1
type: Sequence
persistent identity: IBMc384 seq
display id: IBMc384_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc381_seq/1
type: Sequence
persistent identity: IBMc381 seq
display id: IBMc381_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc226_seq/1
type: Sequence
persistent identity: IBMc226 seq
display id: IBMc226_seq
version: 1
owned by: trevorho
elements: gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaacc…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc425_seq/1
type: Sequence
persistent identity: IBMc425 seq
display id: IBMc425_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc360_seq/1
type: Sequence
persistent identity: IBMc360 seq
display id: IBMc360_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc335_seq/1
type: Sequence
persistent identity: IBMc335 seq
display id: IBMc335_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc387_seq/1
type: Sequence
persistent identity: IBMc387 seq
display id: IBMc387_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc422_seq/1
type: Sequence
persistent identity: IBMc422 seq
display id: IBMc422_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc421_seq/1
type: Sequence
persistent identity: IBMc421 seq
display id: IBMc421_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc474_seq/1
type: Sequence
persistent identity: IBMc474 seq
display id: IBMc474_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc468_seq/1
type: Sequence
persistent identity: IBMc468 seq
display id: IBMc468_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc325_seq/1
type: Sequence
persistent identity: IBMc325 seq
display id: IBMc325_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc395_seq/1
type: Sequence
persistent identity: IBMc395 seq
display id: IBMc395_seq
version: 1
owned by: trevorho
elements: cactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctcgagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaaccgtggctccctcactttctggctggatgatggggcgattcaggcctggtatgagtcagcaacaccttcttcacgaggcagacctcagcgctagcggagtgtatactggcttactatgttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttcccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctc…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc120_seq/1
type: Sequence
persistent identity: IBMc120 seq
display id: IBMc120_seq
version: 1
owned by: trevorho
elements: gtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGCGCGGATAAAAATTTCATTTGCCCGCGACGGATTCCCCGCCCATCTATCGTTGAACCCATACTAGAGATTAAAGAGGAGAAATACTAGatggtgagtaaaggagaagctgtgattaaagagttcatgcgcttcaaagttcacatggagggttctatgaacggtcacgagttcgagatcgaaggcgaaggcgagggccgtccgtatgaaggcacccagaccgccaaactgaaagtgactaaaggcggcccgctgcctttttcctgggacatcctgagcccgcaatttatgtacggttctagggcgttcatcaaacacccagcggatatcccggactattataagcagtcttttccggaaggtttcaagtgggaacgcgtaatgaattttgaagatggtggtgccgtgaccgtcactcaggacacctccctggaggatgg…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc101_seq/1
type: Sequence
persistent identity: IBMc101 seq
display id: IBMc101_seq
version: 1
owned by: trevorho
elements: gagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtttactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcg…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc373_seq/1
type: Sequence
persistent identity: IBMc373 seq
display id: IBMc373_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc408_seq/1
type: Sequence
persistent identity: IBMc408 seq
display id: IBMc408_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtgg…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc454_seq/1
type: Sequence
persistent identity: IBMc454 seq
display id: IBMc454_seq
version: 1
owned by: trevorho
elements: gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaacc…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc405_seq/1
type: Sequence
persistent identity: IBMc405 seq
display id: IBMc405_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc227_seq/1
type: Sequence
persistent identity: IBMc227 seq
display id: IBMc227_seq
version: 1
owned by: trevorho
elements: gggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtttactagaggtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgaga…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc071_seq/1
type: Sequence
persistent identity: IBMc071 seq
display id: IBMc071_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc131_seq/1
type: Sequence
persistent identity: IBMc131 seq
display id: IBMc131_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc483_seq/1
type: Sequence
persistent identity: IBMc483 seq
display id: IBMc483_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc420_seq/1
type: Sequence
persistent identity: IBMc420 seq
display id: IBMc420_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATA…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc458_seq/1
type: Sequence
persistent identity: IBMc458 seq
display id: IBMc458_seq
version: 1
owned by: trevorho
elements: ttacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaAACgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGGTCTCActggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggtTACatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattTGAGACCtactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc481_seq/1
type: Sequence
persistent identity: IBMc481 seq
display id: IBMc481_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc322_seq/1
type: Sequence
persistent identity: IBMc322 seq
display id: IBMc322_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc409_seq/1
type: Sequence
persistent identity: IBMc409 seq
display id: IBMc409_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaagg…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc331_seq/1
type: Sequence
persistent identity: IBMc331 seq
display id: IBMc331_seq
version: 1
owned by: trevorho
elements: ttacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaAACgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGGTCTCAAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGAAAGCGGTCTGATTCATAATCGTATGGTTCAGGCAAGCGATCGTTTTCTGCAATATATTCATCAGGTTCTGCGTCATGCAGTTACCCAGGGTGAACTGCCGATTAATCTGGATCTGCAAACCAGCATTGGTGTTTTTAAAGGTCTGATTACCGGTCTGCTGTATGAAGGTCTGCGTAGCAAAGATCAGCAGGCACAGATTATCAAAGTTGCACTGGGTAGCTTTTGGGCACTGCTGTGAGACCtactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcct…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc176_seq/1
type: Sequence
persistent identity: IBMc176 seq
display id: IBMc176_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc400_seq/1
type: Sequence
persistent identity: IBMc400 seq
display id: IBMc400_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc259_seq/1
type: Sequence
persistent identity: IBMc259 seq
display id: IBMc259_seq
version: 1
owned by: trevorho
elements: aacgcagcggtggtaacggcgcagtggcggttttcatggcttgttatgactgtttttttggggtacagtctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAGACaaTTGTCCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATT…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc190_seq/1
type: Sequence
persistent identity: IBMc190 seq
display id: IBMc190_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc415_seq/1
type: Sequence
persistent identity: IBMc415 seq
display id: IBMc415_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGAAAGCGGTCTGATTCATAATCGTATGGTTCAGGCAAGCGATCGTTTTCTGCAATATATTCATCAGGTTCTGCGTCATGCAGTTACCCAGGGTGAACTGCCGATTAATCTGGATCTGCAAACCAGCATTGGTGTTTTTAAAGGTCTGATTACCGGTCTGCTGTATGAAGGTCTGCGTAGCAAAGATCAGCATGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtcta…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc416_seq/1
type: Sequence
persistent identity: IBMc416 seq
display id: IBMc416_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGAAAGCGGTCTGATTCATAATCGTATGGTTCAGGCAAGCGATCGTTTTCTGCAATATATTCATCAGGTTCTGCGTCATGCAGTTACCCAGGGTGAACTGCCGATTAATCTGGATCTGCAAACCAGCATTGGTGTTTTTAAAGGTCTGATTACCGGTCTGCTGTATGAAGGTCTGCGTAGCAAAGATCAGCAGGCTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc455_seq/1
type: Sequence
persistent identity: IBMc455 seq
display id: IBMc455_seq
version: 1
owned by: trevorho
elements: gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaacc…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc479_seq/1
type: Sequence
persistent identity: IBMc479 seq
display id: IBMc479_seq
version: 1
owned by: trevorho
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgagctcggtaccttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc308_seq/1
type: Sequence
persistent identity: IBMc308 seq
display id: IBMc308_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc383_seq/1
type: Sequence
persistent identity: IBMc383 seq
display id: IBMc383_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc078_seq/1
type: Sequence
persistent identity: IBMc078 seq
display id: IBMc078_seq
version: 1
owned by: trevorho
elements: ttcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtttactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggag…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc447_seq/1
type: Sequence
persistent identity: IBMc447 seq
display id: IBMc447_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgttggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttgggtagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttat…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc410_seq/1
type: Sequence
persistent identity: IBMc410 seq
display id: IBMc410_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGAAAGCGGTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcatt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc187_seq/1
type: Sequence
persistent identity: IBMc187 seq
display id: IBMc187_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc065_seq/1
type: Sequence
persistent identity: IBMc065 seq
display id: IBMc065_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc398_seq/1
type: Sequence
persistent identity: IBMc398 seq
display id: IBMc398_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc411_seq/1
type: Sequence
persistent identity: IBMc411 seq
display id: IBMc411_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTAAACTGGAAGTTCTGCAAGCAGTTCTGGCAAGCCGTCAGCATCCGCTGGAACTGGATTTTACACCGGATCTGGGTATTGAACGTAGCTGGGAAGCAGTTGTTGTTGCAATGCTGGATGCAGTTCATAGTCCGCAGAGCAAACAGTTTAGCGAAATTCTGATTTATCAGGGTCTGGATGAAAGCGGTCTGATTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccac…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc404_seq/1
type: Sequence
persistent identity: IBMc404 seq
display id: IBMc404_seq
version: 1
owned by: trevorho
elements: ttaattaaagcggataacaatttcacacaggaggccgcctaggccgcggccgcgcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagATGGCACGTAAAACCGCAGCAGAAGCAGAAGAAACCCGTCAGCGTATTATTGATGCAGCACTGGAAGTTTTTGTTGCACAGGGTGTTAGTGATGCAACCCTGGATCAGATTGCACGTAAAGCCGGTGTTACCCGTGGTGCAGTTTATTGGCATTTTAATGGTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATT…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc378_seq/1
type: Sequence
persistent identity: IBMc378 seq
display id: IBMc378_seq
version: 1
owned by: trevorho
elements: gagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaa…
encoding: naseq.html

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc380_seq/1
type: Sequence
persistent identity: IBMc380 seq
display id: IBMc380_seq
version: 1
owned by: trevorho
elements: gagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaa…
encoding: naseq.html