LOCUS       SEGA013                 3213 bp    ds-DNA  linear   UNA 09-SEP-2021
DEFINITION  natural linear DNA.
ACCESSION   SEGA013
VERSION     SEGA013.1  
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
FEATURES             Location/Qualifiers
     CDS             1..528
                     /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP
                     IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE
                     VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT
                     DVDQPRNLAKSVTVE"
                     /label="glmS"
                     /codon_start=1
                     /note="glmS"
     terminator      538..594
                     /label="L3S2P11 Terminator"
                     /note="L3S2P11 Terminator"
     promoter        604..638
                     /label="P-J23100"
                     /note="P-J23100"
     RBS             650..676
                     /note="TIR"
                     /note="high TIR"
     CDS             668..688
                     /translation="MENLYFQ"
                     /label="TEV site"
                     /codon_start=1
                     /note="TEV site"
                     /product="tobacco etch virus (TEV) protease recognition 
                     and cleavage site"
     CDS             689..1399
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGI
                     KANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSTLSKDPNEKRDHMV
                     LLEFVTAAGITHGMDELYK"
                     /label="GFP"
                     /codon_start=1
                     /note="GFP"
                     /product="green fluorescent protein"
     terminator      1403..1451
                     /label="L3S3P22 Terminator (synthetic)"
                     /note="L3S3P22 Terminator"
     promoter        1462..1490
                     /label="Promoter 3BCD2"
                     /note="Promoter 3BCD2"
     CDS             1530..2720
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
                     /label="TcR"
                     /codon_start=1
                     /gene="tet"
                     /note="confers resistance to tetracycline"
                     /note="TcR"
                     /product="tetracycline efflux protein"
     terminator      2731..2771
                     /label="rrnC"
                     /note="rrnC"
     CDS             2966..3213
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA"
                     /label="pstS"
                     /codon_start=1
                     /note="pstS"
ORIGIN
        1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc
       61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc
      121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac
      181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat
      241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt
      301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt
      361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg
      421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc
      481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc
      541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg
      601 cgcttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta aagaggagaa
      661 aatatcgatg gagaacctgt acttccagag caaaggagaa gaacttttca ctggagttgt
      721 gccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      781 gggtgaaggt gatgctacat acggaaaact cacccttaaa tttatttgca ctactggaaa
      841 actacctgtt ccatggccaa cacttgtcac tactctgacc tatggcgttc aatgcttttc
      901 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
      961 tgtacaggaa cgcactatat ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt
     1021 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga
     1081 agatggaaac attctcggac acaaactaga gtacaactat aactcacaca atgtatacat
     1141 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacattga
     1201 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     1261 tgtcctttta ccagacaacc attacctgtc gacacaatct accctttcga aagatcccaa
     1321 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg
     1381 catggatgag ctctacaaat gaccaattat tgaaggccgc taacgcggcc tttttttgtt
     1441 tctggtctcc caaaaattta tttgcttatt aatcatccgg ctcgtataat gtgtggaggg
     1501 cccaagttca cttaaaaagg agaatagtta tgaaatcgaa caatgcgctc atcgtcatcc
     1561 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
     1621 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc
     1681 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg
     1741 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg
     1801 cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggccggc atcaccggcg
     1861 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     1921 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     1981 ggggactgtt gggcgccatc tccctgcacg caccattcct tgcggcggcg gtgctcaacg
     2041 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgccgtc
     2101 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacca
     2161 ttgtggccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2221 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg agcggcctgt
     2281 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     2341 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     2401 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     2461 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     2521 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg
     2581 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     2641 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     2701 gccgggccac ctcgacctga gacaattgtc cagataaaaa aaatccttag ctttcgctaa
     2761 ggatgatttc taaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat
     2821 atcaacgctt atttaaatca gactgaagac tttatctctc tgtcataaaa ctgtcatatt
     2881 ccttacatat aactgtcacc tgtttgtcct attttgcttc tcgtagccaa caaacaatgc
     2941 tttatgaatc ctcccaggag acattatgaa agttatgcgt accaccgtcg caactgttgt
     3001 cgccgcgacc ttatcgatga gtgctttctc tgtgtttgca gaagcaagcc tgacaggtgc
     3061 aggtgcaacc ttccctgcgc cggtgtatgc caaatgggct gacacttacc agaaagaaac
     3121 cggtaataaa gttaactacc agggtatcgg ttcttccggt ggcgtaaaac agattatcgc
     3181 taataccgtt gattttggtg cctctgacgc gcc
//