##gff-version 3
##sequence-region SEGA029 1 4459
SEGA029	.	promoter	2708	2736	.	+	0	ID=annotation11;Name=Promoter 3BCD2
SEGA029	.	promoter	1795	1824	.	+	0	ID=annotation4;Name=The trc promoter combines the -35 region from the trp promoter and the -10 region from the lacUV5 promoter
SEGA029	.	terminator	3977	4017	.	+	0	ID=annotation13;Name=rrnC
SEGA029	.	CDS	1878	1898	.	+	0	ID=annotation7;Name=TEV site
SEGA029	.	sequence_feature	1825	1848	.	+	0	ID=annotation5;Name=lacO
SEGA029	.	biological_region	1899	1934	.	+	0	ID=annotation8;Name=PROTi-Linker
SEGA029	.	CDS	1935	2645	.	+	0	ID=annotation9;Name=GFP
SEGA029	.	terminator	538	594	.	+	0	ID=annotation1;Name=L3S2P11 Terminator
SEGA029	.	CDS	604	1686	.	-	0	ID=annotation2;Name=lacI
SEGA029	.	CDS	4212	4459	.	+	0	ID=annotation14;Name=pstS
SEGA029	.	CDS	2776	3966	.	+	0	ID=annotation12;Name=TcR
SEGA029	.	CDS	1	528	.	+	0	ID=annotation0;Name=glmS
SEGA029	.	promoter	1687	1764	.	-	0	ID=annotation3;Name=lacI promoter
SEGA029	.	ribosome_entry_site	1860	1886	.	+	0	ID=annotation6;Name=low TIR
SEGA029	.	terminator	2649	2697	.	+	0	ID=annotation10;Name=L3S3P22 Terminator (synthetic)
>SEGA029
gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaag
atttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagtt
gaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgcc
gatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgc
gtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgcc
gcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaa
ggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattcc
agaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacct
gtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttct
tttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctg
gtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgt
atcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatc
gttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactc
cagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcg
ccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcg
cgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacatta
gtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcga
gaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttg
atcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagc
aacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttttt
cccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgc
gacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcga
aaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcg
tataatggaattgtgagcggataacaattcctactagagattaaagaggagaaacgggggatggagaacctgtacttcc
agtttcgtagcaaaggagaagaacttgtcactggagttagcaaaggagaagaacttttcactggagttgtgccaattct
tgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaa
ctcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcg
ttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtaca
ggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgtt
aatcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataact
cacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaaga
tggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccat
tacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactg
ctgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgtt
tctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaag
gagaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttg
gttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctag
cgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcct
gctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccgga
cgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatc
gggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttggg
cgccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatg
caggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggg
gcatgaccattgtggccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggt
cattttcggcgaggaccgctttcgctggagcgcgacgatgagcggcctgtcgcttgcggtattcggaatcttgcacgcc
ctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccg
acgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccgg
cggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcg
ctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacat
ggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccg
ggccacctcgacctgagacaattgtccagataaaaaaaatccttagctttcgctaaggatgatttctaaaatttttctg
tcatctcttcgttattaatgtttgtaattgactgaatatcaacgcttatttaaatcagactgaagactttatctctctg
tcataaaactgtcatattccttacatataactgtcacctgtttgtcctattttgcttctcgtagccaacaaacaatgct
ttatgaatcctcccaggagacattatgaaagttatgcgtaccaccgtcgcaactgttgtcgccgcgaccttatcgatga
gtgctttctctgtgtttgcagaagcaagcctgacaggtgcaggtgcaaccttccctgcgccggtgtatgccaaatgggc
tgacacttaccagaaagaaaccggtaataaagttaactaccagggtatcggttcttccggtggcgtaaaacagattatc
gctaataccgttgattttggtgcctctgacgcgcc