LOCUS SEGA034 4468 bp ds-DNA linear UNA 08-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA034 VERSION SEGA034.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(604..1686) /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label="lacI" /codon_start=1 /gene="lacI" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalacto pyranoside (IPTG)." /product="lac repressor" promoter complement(1687..1764) /label="lacI promoter" /gene="lacI" /note="lacI promoter" promoter 1795..1824 /label="trc promoter" /note="trc promoter" /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 1825..1848 /bound_moiety="lac repressor" /label="lacO" /note="lacO" /note="lac operator" /featureType="protein_bind" RBS 1860..1886 /note="high TIR" /note="TIR" CDS 1878..1943 /translation="MKYLLPTAAAGLLLLAAQPAMA" /label="pelB signal sequence" /codon_start=1 /gene="pelB (fragment)" /note="pelB signal sequence" /product="leader peptide for secretion" CDS 1944..2654 /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGI KANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="sfGFP" /codon_start=1 /note="sfGFP" /product="superfolder GFP" terminator 2658..2706 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 2717..2745 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 2785..3975 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="TcR" /note="confers resistance to tetracycline" /product="tetracycline efflux protein" terminator 3986..4026 /label="rrnC" /note="rrnC" CDS 4221..4468 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 721 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 781 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 841 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 901 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 961 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 1021 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 1081 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 1141 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 1201 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 1261 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 1321 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 1381 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 1441 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 1501 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 1561 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 1621 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 1681 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 1741 ggttttgcgc cattcgatgg tgtcggatcg agatcgatct cgatcccgcg aaatttgaca 1801 attaatcatc cggctcgtat aatggaattg tgagcggata acaattccta ctagagatta 1861 aagaggagaa attaattatg aaatatctgc tgccgaccgc tgctgctggt ctgctgctcc 1921 tcgctgccca gccggcgatg gcccgtaaag gcgaagagct gttcactggt gtcgtcccta 1981 ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg 2041 aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc 2101 cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt 2161 atccggacca catgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc 2221 aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat 2281 ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg 2341 gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg 2401 ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg 2461 gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc 2521 tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga 2581 aacgcgatca catggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg 2641 atgaactgta caaatgacca attattgaag gccgctaacg cggccttttt ttgtttctgg 2701 tctcccaaaa atttatttgc ttattaatca tccggctcgt ataatgtgtg gagggcccaa 2761 gttcacttaa aaaggagaat agttatgaaa tcgaacaatg cgctcatcgt catcctcggc 2821 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg 2881 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat 2941 gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc 3001 cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc 3061 acacccgtcc tgtggattct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca 3121 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac 3181 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga 3241 ctgttgggcg ccatctccct gcacgcacca ttccttgcgg cggcggtgct caacggcctc 3301 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg ccgtccgatg 3361 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gaccattgtg 3421 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc 3481 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgagcgg cctgtcgctt 3541 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa 3601 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc 3661 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc 3721 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat 3781 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg 3841 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt 3901 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg 3961 gccacctcga cctgagacaa ttgtccagat aaaaaaaatc cttagctttc gctaaggatg 4021 atttctaaaa tttttctgtc atctcttcgt tattaatgtt tgtaattgac tgaatatcaa 4081 cgcttattta aatcagactg aagactttat ctctctgtca taaaactgtc atattcctta 4141 catataactg tcacctgttt gtcctatttt gcttctcgta gccaacaaac aatgctttat 4201 gaatcctccc aggagacatt atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg 4261 cgaccttatc gatgagtgct ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg 4321 caaccttccc tgcgccggtg tatgccaaat gggctgacac ttaccagaaa gaaaccggta 4381 ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt atcgctaata 4441 ccgttgattt tggtgcctct gacgcgcc //