LOCUS SEGA035 4756 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA035 VERSION SEGA035.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(604..1686) /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label="lacI" /codon_start=1 /gene="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalacto pyranoside (IPTG)." /note="lacI" /product="lac repressor" promoter complement(1687..1764) /label="lacI promoter" /gene="lacI" /note="lacI promoter" promoter 1795..1824 /label="trc promoter" /note="trc promoter" /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 1825..1848 /bound_moiety="lac repressor" /label="lacO" /note="lac operator" /note="lacO" /featureType="protein_bind" RBS 1860..1886 /note="TIR" /note="low TIR" CDS 1878..1940 /protein_id="NP_416361.2" /translation="MKKRGAFLGLLLVSACASVFA" /label="YebFss" /gene_synonym="ECK1848; JW1836" /codon_start=1 /db_xref="GeneID:946363" /db_xref="ASAP:ABE-0006141" /db_xref="EcoGene:EG11807" /db_xref="UniProtKB/Swiss-Prot:P33219" /db_xref="GI:145698278" /gene="yebF" /note="YebFss" /product="yebF signal sequence" /locus_tag="b1847" CDS 1941..2231 /translation="ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKI VGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR" /label="YebF" /codon_start=1 /note="YebF" /product="full size yebF protein" CDS 2232..2942 /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGI KANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="sfGFP" /codon_start=1 /note="sfGFP" /product="superfolder GFP" terminator 2946..2994 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 3005..3033 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 3073..4263 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="TcR" /note="confers resistance to tetracycline" /product="tetracycline efflux protein" terminator 4274..4314 /label="rrnC" /note="rrnC" CDS 4509..4756 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 721 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 781 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 841 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 901 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 961 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 1021 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 1081 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 1141 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 1201 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 1261 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 1321 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 1381 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 1441 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 1501 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 1561 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 1621 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 1681 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 1741 ggttttgcgc cattcgatgg tgtcggatcg agatcgatct cgatcccgcg aaatttgaca 1801 attaatcatc cggctcgtat aatggaattg tgagcggata acaattccta ctagagatta 1861 aagaggagaa accctcgatg aagaaaagag gggcgttttt agggctgttg ttggtttctg 1921 cctgcgcatc agttttcgct gcgaataacg aaacgagcaa gagcgtcacc ttcccgaagt 1981 gcgaggatct ggacgcagcg ggcattgctg ccagcgtgaa acgtgactac caacagaacc 2041 gcgtggctcg ttgggcagac gaccagaaga tcgtcggtca agcggacccg gttgcctggg 2101 tttccctgca agatatccag ggcaaagatg ataaatggag cgtcccgctg accgttcgtg 2161 gtaagagcgc ggacattcac tatcaggtga gcgtcgattg caaagccggc atggcggagt 2221 accaacgccg ccgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac 2281 tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa 2341 ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc 2401 cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccaca 2461 tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga 2521 tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata 2581 ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg 2641 gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa 2701 aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc 2761 tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca 2821 atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcaca 2881 tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca 2941 aatgaccaat tattgaaggc cgctaacgcg gccttttttt gtttctggtc tcccaaaaat 3001 ttatttgctt attaatcatc cggctcgtat aatgtgtgga gggcccaagt tcacttaaaa 3061 aggagaatag ttatgaaatc gaacaatgcg ctcatcgtca tcctcggcac cgtcaccctg 3121 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc 3181 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa 3241 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg 3301 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg 3361 tggattctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct 3421 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg 3481 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc 3541 atctccctgc acgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg 3601 ggctgcttcc taatgcagga gtcgcataag ggagagcgcc gtccgatgcc cttgagagcc 3661 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ccattgtggc cgcacttatg 3721 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc 3781 ggcgaggacc gctttcgctg gagcgcgacg atgagcggcc tgtcgcttgc ggtattcgga 3841 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag 3901 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc 3961 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg 4021 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt 4081 caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg 4141 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc 4201 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc 4261 tgagacaatt gtccagataa aaaaaatcct tagctttcgc taaggatgat ttctaaaatt 4321 tttctgtcat ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttaaa 4381 tcagactgaa gactttatct ctctgtcata aaactgtcat attccttaca tataactgtc 4441 acctgtttgt cctattttgc ttctcgtagc caacaaacaa tgctttatga atcctcccag 4501 gagacattat gaaagttatg cgtaccaccg tcgcaactgt tgtcgccgcg accttatcga 4561 tgagtgcttt ctctgtgttt gcagaagcaa gcctgacagg tgcaggtgca accttccctg 4621 cgccggtgta tgccaaatgg gctgacactt accagaaaga aaccggtaat aaagttaact 4681 accagggtat cggttcttcc ggtggcgtaa aacagattat cgctaatacc gttgattttg 4741 gtgcctctga cgcgcc //