##gff-version 3 ##sequence-region SEGA035 1 4756 SEGA035 . CDS 3073 4263 . + 0 ID=annotation12;Name=TcR SEGA035 . CDS 1878 1940 . + 0 ID=annotation7;Name=YebFss SEGA035 . terminator 538 594 . + 0 ID=annotation1;Name=L3S2P11 Terminator SEGA035 . CDS 2232 2942 . + 0 ID=annotation9;Name=sfGFP SEGA035 . CDS 4509 4756 . + 0 ID=annotation14;Name=pstS SEGA035 . terminator 2946 2994 . + 0 ID=annotation10;Name=L3S3P22 Terminator (synthetic) SEGA035 . sequence_feature 1825 1848 . + 0 ID=annotation5;Name=lacO SEGA035 . CDS 604 1686 . - 0 ID=annotation2;Name=lacI SEGA035 . CDS 1 528 . + 0 ID=annotation0;Name=glmS SEGA035 . terminator 4274 4314 . + 0 ID=annotation13;Name=rrnC SEGA035 . promoter 3005 3033 . + 0 ID=annotation11;Name=Promoter 3BCD2 SEGA035 . CDS 1941 2231 . + 0 ID=annotation8;Name=YebF SEGA035 . ribosome_entry_site 1860 1886 . + 0 ID=annotation6;Name=low TIR SEGA035 . promoter 1687 1764 . - 0 ID=annotation3;Name=lacI promoter SEGA035 . promoter 1795 1824 . + 0 ID=annotation4;Name=trc promoter >SEGA035 gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaag atttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagtt gaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgcc gatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgc gtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgcc gcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaa ggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattcc agaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacct gtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttct tttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctg gtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgt atcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatc gttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactc cagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcg ccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcg cgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacatta gtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcga gaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttg atcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagc aacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttttt cccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgc gacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcga aaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcg tataatggaattgtgagcggataacaattcctactagagattaaagaggagaaaccctcgatgaagaaaagaggggcgt ttttagggctgttgttggtttctgcctgcgcatcagttttcgctgcgaataacgaaacgagcaagagcgtcaccttccc gaagtgcgaggatctggacgcagcgggcattgctgccagcgtgaaacgtgactaccaacagaaccgcgtggctcgttgg gcagacgaccagaagatcgtcggtcaagcggacccggttgcctgggtttccctgcaagatatccagggcaaagatgata aatggagcgtcccgctgaccgttcgtggtaagagcgcggacattcactatcaggtgagcgtcgattgcaaagccggcat ggcggagtaccaacgccgccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgat gtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatct gtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgtta tccggaccacatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttccttt aaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaag gcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcac cgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggct gatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcg ttctgtctaaagatccgaacgagaaacgcgatcacatggttctgctggagttcgtaaccgcagcgggcatcacgcatgg tatggatgaactgtacaaatgaccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaattt atttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcg aacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgg gcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgca atttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttgga gccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatca ccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggct catgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccctgcacgca ccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggag agcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgaccattgtggccgc acttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgc tttcgctggagcgcgacgatgagcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtca ctggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtctt gctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcg ttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcc taacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggat tgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgagac aattgtccagataaaaaaaatccttagctttcgctaaggatgatttctaaaatttttctgtcatctcttcgttattaat gtttgtaattgactgaatatcaacgcttatttaaatcagactgaagactttatctctctgtcataaaactgtcatattc cttacatataactgtcacctgtttgtcctattttgcttctcgtagccaacaaacaatgctttatgaatcctcccaggag acattatgaaagttatgcgtaccaccgtcgcaactgttgtcgccgcgaccttatcgatgagtgctttctctgtgtttgc agaagcaagcctgacaggtgcaggtgcaaccttccctgcgccggtgtatgccaaatgggctgacacttaccagaaagaa accggtaataaagttaactaccagggtatcggttcttccggtggcgtaaaacagattatcgctaataccgttgattttg gtgcctctgacgcgcc