LOCUS SEGA043 5489 bp ds-DNA linear UNA 08-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA043 VERSION SEGA043.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(607..1452) /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG AIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH LNSQKD" /label="rhaR" /codon_start=1 /gene="E. coli rhaR" /note="needed for rhamnose-dependent activation of the rhaB promoter" /note="rhaR" /product="transcriptional activator of rhaR and rhaS" CDS complement(1526..2362) /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF LQ" /label="rhaS" /codon_start=1 /gene="E. coli rhaS" /note="activated by L-rhamnose" /note="rhaS" /product="positive regulator of the rhaB promoter" promoter 2508..2626 /label="rhaB promoter" /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /note="rhaB promoter" RBS 2638..2664 /note="TIR" /note="high TIR" CDS 2656..2955 /translation="MQIFVKTLTGKTITLEVEPGSAHHHHHHHHHHAGSSDTIENVKA KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQEKSTLELVLELQNETQSATSDA" /label="Ubiquitin" /codon_start=1 /note="Ubiquitin" CDS 2965..3675 /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGI KANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="GFP" /codon_start=1 /note="GFP" /product="green fluorescent protein" terminator 3679..3727 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 3738..3766 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 3806..4996 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="confers resistance to tetracycline" /note="TcR" /product="tetracycline efflux protein" terminator 5007..5047 /label="rrnC" /note="rrnC" CDS 5242..5489 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgcttaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg 661 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact 721 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgtcgcag 781 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact 841 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag 901 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt 961 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt 1021 agcaaacggc acatgctgac tactttcatg ctcaagctga ccaataacct gccgcgcctg 1081 cgccatcccc atgctaccta agcgccagtg tggttgccct gcgctggcgt taaatcccgg 1141 aatcgccccc tgccagtcaa gattcagctt cagacgctcc gggcaataaa taatattctg 1201 caaaaccaga tcgttaacgg aagcgtagga gtgtttatca tcagcatgaa tgtaaaagag 1261 atcgccacgg gtaatgcgat aagggcgatc gttgagtaca tgcaggccat taccgcgcca 1321 gacaatcacc agctcacaaa aatcatgtgt atgttcagca aagacatctt gcggataacg 1381 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa 1441 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg 1501 tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat 1561 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga 1621 atccacagcg ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc agatgtcggg 1681 ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa 1741 gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca 1801 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt 1861 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga 1921 tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa 1981 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc 2041 catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg 2101 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat 2161 gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat 2221 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa 2281 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttaccagac ggaaaaaaat 2341 ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa 2401 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt 2461 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag 2521 caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca ttttcttgtc 2581 agtaacgaga aggtcgcgaa tccaggcgct ttttagactg gtcgtatact agagattaaa 2641 gaggagaaac attgtatgca gatattcgtg aaaacactga ccggcaaaac cattaccctg 2701 gaggtggagc ctggcagcgc ccatcatcat caccatcacc atcaccatca tgctggtagc 2761 agcgatacga tcgaaaacgt caaagccaaa attcaggaca aagagggcat tccgcctgat 2821 caacaacgtc tgatcttcgc cggaaaacag ctggaggatg gccgtacact gagtgactat 2881 aacattcaag agaaatcgac actggaactg gttctggagc tgcagaatga aacccagtca 2941 gcaacttctg acgcttctgg cggtagcaaa ggagaagaac ttttcactgg agttgtgcca 3001 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt 3061 gaaggtgatg ctacatacgg aaaactcacc cttaaattta tttgcactac tggaaaacta 3121 cctgttccat ggccaacact tgtcactact ctgacctatg gcgttcaatg cttttcccgt 3181 tatccggatc acatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta 3241 caggaacgca ctatatcttt caaagatgac gggaactaca agacgcgtgc tgaagtcaag 3301 tttgaaggtg atacccttgt taatcgtatc gagttaaagg gtattgattt taaagaagat 3361 ggaaacattc tcggacacaa actagagtac aactataact cacacaatgt atacatcacg 3421 gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa cattgaagat 3481 ggttccgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc 3541 cttttaccag acaaccatta cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa 3601 aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac acatggcatg 3661 gatgagctct acaaatgacc aattattgaa ggccgctaac gcggcctttt tttgtttctg 3721 gtctcccaaa aatttatttg cttattaatc atccggctcg tataatgtgt ggagggccca 3781 agttcactta aaaaggagaa tagttatgaa atcgaacaat gcgctcatcg tcatcctcgg 3841 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 3901 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 3961 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 4021 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 4081 cacacccgtc ctgtggattc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 4141 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 4201 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 4261 actgttgggc gccatctccc tgcacgcacc attccttgcg gcggcggtgc tcaacggcct 4321 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gccgtccgat 4381 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgaccattgt 4441 ggccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 4501 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgagcg gcctgtcgct 4561 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 4621 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 4681 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 4741 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 4801 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 4861 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 4921 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 4981 ggccacctcg acctgagaca attgtccaga taaaaaaaat ccttagcttt cgctaaggat 5041 gatttctaaa atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca 5101 acgcttattt aaatcagact gaagacttta tctctctgtc ataaaactgt catattcctt 5161 acatataact gtcacctgtt tgtcctattt tgcttctcgt agccaacaaa caatgcttta 5221 tgaatcctcc caggagacat tatgaaagtt atgcgtacca ccgtcgcaac tgttgtcgcc 5281 gcgaccttat cgatgagtgc tttctctgtg tttgcagaag caagcctgac aggtgcaggt 5341 gcaaccttcc ctgcgccggt gtatgccaaa tgggctgaca cttaccagaa agaaaccggt 5401 aataaagtta actaccaggg tatcggttct tccggtggcg taaaacagat tatcgctaat 5461 accgttgatt ttggtgcctc tgacgcgcc //