https://synbiohub.org/public/SEGA/SEGA064/1
type: ComponentDefinition
persistent identity: SEGA064
display id: SEGA064
version: 1
description: natural linear DNA.
molecule: ds-DNA
keywords: .
locus: SEGA064
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: DnaRegion
type: SO:0000987
role: SO:0000804
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI
sequence annotation: lacI promoter
sequence annotation: T7 promoter
sequence annotation: lac operator
sequence annotation: high TIR
sequence annotation: TEV site
sequence annotation: GFP
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA064_seq/1

https://synbiohub.org/public/SEGA/reference0_1/1
type: Reference
top level: https://synbiohub.org/public/SEGA/SEGA022/1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
authors: Morten Norholm Group
journal: Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com
label: 1 (bases 1 to 4458)
title: Direct Submission

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
label: glmS
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
codon start: 1
note: glmS
location: https://synbiohub.org/public/SEGA/SEGA064/annotation0/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA064/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
note: L3S2P11 Terminator
location: https://synbiohub.org/public/SEGA/SEGA064/annotation1/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA064/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
start: 538
end: 594
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
label: lacI
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
codon start: 1
gene: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
location: https://synbiohub.org/public/SEGA/SEGA064/annotation2/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA064/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
start: 604
end: 1686
orientation: reverseComplement

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: lacI promoter
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
gene: lacI
note: lacI promoter
location: https://synbiohub.org/public/SEGA/SEGA064/annotation3/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA064/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
start: 1687
end: 1764
orientation: reverseComplement

T7 promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: T7 promoter
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
note: promoter for bacteriophage T7 RNA polymerase
note: T7 promoter
location: https://synbiohub.org/public/SEGA/SEGA064/annotation4/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA064/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
start: 1795
end: 1813
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor encoded by lacI
label: lac operator
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lac operator
feature type: protein_bind
location: https://synbiohub.org/public/SEGA/SEGA064/annotation5/range/1
role: SO:0000110

https://synbiohub.org/public/SEGA/SEGA064/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
start: 1814
end: 1838
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
note: TIR
note: high TIR
location: https://synbiohub.org/public/SEGA/SEGA064/annotation6/range/1
role: SO:0000139

https://synbiohub.org/public/SEGA/SEGA064/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
start: 1850
end: 1876
orientation: inline

TEV site
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
translation: MENLYFQ
label: TEV site
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
codon start: 1
note: TEV site
product: tobacco etch virus (TEV) protease recognition and cleavage site
location: https://synbiohub.org/public/SEGA/SEGA064/annotation7/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA064/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
start: 1868
end: 1888
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
label: GFP
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
codon start: 1
note: GFP
product: green fluorescent protein
location: https://synbiohub.org/public/SEGA/SEGA064/annotation8/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA064/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
start: 1889
end: 2599
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
note: L3S3P22 Terminator
location: https://synbiohub.org/public/SEGA/SEGA064/annotation9/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA064/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
start: 2603
end: 2651
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
note: Promoter 3BCD2
location: https://synbiohub.org/public/SEGA/SEGA064/annotation10/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA064/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
start: 2662
end: 2690
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
label: TcR
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
codon start: 1
gene: tet
note: confers resistance to tetracycline
note: TcR
product: tetracycline efflux protein
location: https://synbiohub.org/public/SEGA/SEGA064/annotation11/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA064/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
start: 2730
end: 3920
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
note: rrnC
location: https://synbiohub.org/public/SEGA/SEGA064/annotation12/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA064/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
start: 3931
end: 3971
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
label: pstS
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: carbay
owned by: morno
codon start: 1
note: pstS
location: https://synbiohub.org/public/SEGA/SEGA064/annotation13/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA064/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA064/1
owned by: morno
owned by: carbay
start: 4166
end: 4413
orientation: inline

https://synbiohub.org/public/SEGA/SEGA021/1
type: ComponentDefinition
persistent identity: SEGA021
display id: SEGA021
version: 1
description: natural linear DNA.
molecule: ds-DNA
keywords: .
locus: SEGA021
owned by: carbay
owned by: morno
date: 08-SEP-2021
division: UNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: SO:0000987
type: DnaRegion
role: SO:0000804
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI
sequence annotation: lacI promoter
sequence annotation: trc promoter
sequence annotation: lacO
sequence annotation: medium TIR
sequence annotation: BCD
sequence annotation: GFP
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA021_seq/1

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
label: glmS
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
codon start: 1
note: glmS
location: https://synbiohub.org/public/SEGA/SEGA021/annotation0/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA021/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
note: L3S2P11 Terminator
location: https://synbiohub.org/public/SEGA/SEGA021/annotation1/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA021/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 538
end: 594
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
label: lacI
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
codon start: 1
gene: lacI
note: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
product: lac repressor
location: https://synbiohub.org/public/SEGA/SEGA021/annotation2/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA021/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
start: 604
end: 1686
orientation: reverseComplement

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: lacI promoter
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
gene: lacI
note: lacI promoter
location: https://synbiohub.org/public/SEGA/SEGA021/annotation3/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA021/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 1687
end: 1764
orientation: reverseComplement

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
note: trc promoter
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
location: https://synbiohub.org/public/SEGA/SEGA021/annotation4/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA021/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 1795
end: 1824
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
label: lacO
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
note: lac operator
note: lacO
feature type: protein_bind
location: https://synbiohub.org/public/SEGA/SEGA021/annotation5/range/1
role: SO:0000110

https://synbiohub.org/public/SEGA/SEGA021/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
start: 1825
end: 1848
orientation: inline

medium TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
note: medium TIR
note: TIR
location: https://synbiohub.org/public/SEGA/SEGA021/annotation6/range/1
role: SO:0000139

https://synbiohub.org/public/SEGA/SEGA021/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
start: 1860
end: 1886
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
translation: MKAIFVLKHLNHAKEVF
label: BCD
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
codon start: 1
note: BCD
location: https://synbiohub.org/public/SEGA/SEGA021/annotation7/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA021/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 1878
end: 1933
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
label: GFP
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
codon start: 1
note: GFP
product: green fluorescent protein
location: https://synbiohub.org/public/SEGA/SEGA021/annotation8/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA021/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 1934
end: 2644
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
note: L3S3P22 Terminator
location: https://synbiohub.org/public/SEGA/SEGA021/annotation9/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA021/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
start: 2648
end: 2696
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
note: Promoter 3BCD2
location: https://synbiohub.org/public/SEGA/SEGA021/annotation10/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA021/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 2707
end: 2735
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
label: TcR
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
codon start: 1
gene: tet
note: TcR
note: confers resistance to tetracycline
product: tetracycline efflux protein
location: https://synbiohub.org/public/SEGA/SEGA021/annotation11/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA021/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 2775
end: 3965
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
note: rrnC
location: https://synbiohub.org/public/SEGA/SEGA021/annotation12/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA021/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: morno
owned by: carbay
start: 3976
end: 4016
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
label: pstS
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
codon start: 1
note: pstS
location: https://synbiohub.org/public/SEGA/SEGA021/annotation13/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA021/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA021/1
owned by: carbay
owned by: morno
start: 4211
end: 4458
orientation: inline

https://synbiohub.org/public/SEGA/SEGA022/1
type: ComponentDefinition
persistent identity: SEGA022
display id: SEGA022
version: 1
description: natural linear DNA.
molecule: ds-DNA
keywords: .
locus: SEGA022
owned by: morno
owned by: carbay
date: 09-SEP-2021
division: UNA
organism: unspecified
reference: https://synbiohub.org/public/SEGA/reference0_1/1
source: natural DNA sequence
type: SO:0000987
type: DnaRegion
role: SO:0000804
sequence annotation: glmS
sequence annotation: L3S2P11 Terminator
sequence annotation: lacI
sequence annotation: lacI promoter
sequence annotation: trc promoter
sequence annotation: lacO
sequence annotation: high TIR
sequence annotation: BCD
sequence annotation: GFP
sequence annotation: L3S3P22 Terminator (synthetic)
sequence annotation: Promoter 3BCD2
sequence annotation: TcR
sequence annotation: rrnC
sequence annotation: pstS
sequence: https://synbiohub.org/public/SEGA/SEGA022_seq/1

glmS
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
translation: VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE
label: glmS
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: carbay
owned by: morno
codon start: 1
note: glmS
location: https://synbiohub.org/public/SEGA/SEGA022/annotation0/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA022/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: carbay
owned by: morno
start: 1
end: 528
orientation: inline

L3S2P11 Terminator
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
label: L3S2P11 Terminator
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: carbay
owned by: morno
note: L3S2P11 Terminator
location: https://synbiohub.org/public/SEGA/SEGA022/annotation1/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA022/annotation1/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: carbay
owned by: morno
start: 538
end: 594
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
translation: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
label: lacI
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
codon start: 1
gene: lacI
note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
note: lacI
product: lac repressor
location: https://synbiohub.org/public/SEGA/SEGA022/annotation2/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA022/annotation2/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 604
end: 1686
orientation: reverseComplement

lacI promoter
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
label: lacI promoter
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
gene: lacI
note: lacI promoter
location: https://synbiohub.org/public/SEGA/SEGA022/annotation3/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA022/annotation3/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 1687
end: 1764
orientation: reverseComplement

trc promoter
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
label: trc promoter
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
note: strong E. coli promoter; hybrid between the trp and lac UV5 promoters
note: trc promoter
location: https://synbiohub.org/public/SEGA/SEGA022/annotation4/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA022/annotation4/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 1795
end: 1824
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
bound moiety: lac repressor
label: lacO
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
note: lac operator
note: lacO
feature type: protein_bind
location: https://synbiohub.org/public/SEGA/SEGA022/annotation5/range/1
role: SO:0000110

https://synbiohub.org/public/SEGA/SEGA022/annotation5/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 1825
end: 1848
orientation: inline

high TIR
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
note: TIR
note: high TIR
location: https://synbiohub.org/public/SEGA/SEGA022/annotation6/range/1
role: SO:0000139

https://synbiohub.org/public/SEGA/SEGA022/annotation6/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 1860
end: 1886
orientation: inline

BCD
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
translation: MKAIFVLKHLNHAKEVF
label: BCD
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
codon start: 1
note: BCD
location: https://synbiohub.org/public/SEGA/SEGA022/annotation7/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA022/annotation7/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 1878
end: 1933
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
translation: SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
label: GFP
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
codon start: 1
note: GFP
product: green fluorescent protein
location: https://synbiohub.org/public/SEGA/SEGA022/annotation8/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA022/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 1934
end: 2644
orientation: inline

L3S3P22 Terminator (synthetic)
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
label: L3S3P22 Terminator (synthetic)
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
note: L3S3P22 Terminator
location: https://synbiohub.org/public/SEGA/SEGA022/annotation9/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA022/annotation9/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 2648
end: 2696
orientation: inline

Promoter 3BCD2
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
label: Promoter 3BCD2
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: carbay
owned by: morno
note: Promoter 3BCD2
location: https://synbiohub.org/public/SEGA/SEGA022/annotation10/range/1
role: SO:0000167

https://synbiohub.org/public/SEGA/SEGA022/annotation10/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 2707
end: 2735
orientation: inline

TcR
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
translation: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST
label: TcR
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
codon start: 1
gene: tet
note: confers resistance to tetracycline
note: TcR
product: tetracycline efflux protein
location: https://synbiohub.org/public/SEGA/SEGA022/annotation11/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA022/annotation11/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 2775
end: 3965
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
label: rrnC
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
note: rrnC
location: https://synbiohub.org/public/SEGA/SEGA022/annotation12/range/1
role: SO:0000141

https://synbiohub.org/public/SEGA/SEGA022/annotation12/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 3976
end: 4016
orientation: inline

pstS
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
translation: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA
label: pstS
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
codon start: 1
note: pstS
location: https://synbiohub.org/public/SEGA/SEGA022/annotation13/range/1
role: SO:0000316

https://synbiohub.org/public/SEGA/SEGA022/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/SEGA/SEGA022/1
owned by: morno
owned by: carbay
start: 4211
end: 4458
orientation: inline

https://synbiohub.org/public/SEGA/SEGA064_seq/1
type: Sequence
persistent identity: SEGA064 seq
display id: SEGA064_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcctactagagattaaagaggagaaaatatcgatggagaacctgtacttccagagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacat…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA021_seq/1
type: Sequence
persistent identity: SEGA021 seq
display id: SEGA021_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaagagctaatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatg…
encoding: naseq.html

https://synbiohub.org/public/SEGA/SEGA022_seq/1
type: Sequence
persistent identity: SEGA022 seq
display id: SEGA022_seq
version: 1
owned by: carbay
owned by: morno
elements: gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaagatttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagttgaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgccgatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgcgtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgccgcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaaggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattccagaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcgtataatggaattgtgagcggataacaattcctactagagattaaagaggagaaactatatatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatg…
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