LOCUS SEGA066 4449 bp ds-DNA linear UNA 08-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA066 VERSION SEGA066.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(604..1686) /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label="lacI" /codon_start=1 /gene="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalacto pyranoside (IPTG)." /note="lacI" /product="lac repressor" promoter complement(1687..1764) /label="lacI promoter" /gene="lacI" /note="lacI promoter" promoter 1795..1813 /label="T7 promoter" /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 1814..1838 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalacto pyranoside (IPTG)." /featureType="protein_bind" RBS 1850..1876 /note="TIR" /note="medium TIR" CDS 1868..1888 /translation="MENLYFQ" /label="TEV site" /codon_start=1 /note="TEV site" /product="tobacco etch virus (TEV) protease recognition and cleavage site" misc_feature 1889..1924 /label="PROTi-Linker" /note="PROTi-Linker" CDS 1925..2635 /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGI KANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="GFP" /codon_start=1 /note="GFP" /product="green fluorescent protein" terminator 2639..2687 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 2698..2726 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 2766..3956 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="confers resistance to tetracycline" /note="TcR" /product="tetracycline efflux protein" terminator 3967..4007 /label="rrnC" /note="rrnC" CDS 4202..4449 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 721 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 781 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 841 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 901 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 961 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 1021 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 1081 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 1141 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 1201 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 1261 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 1321 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 1381 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 1441 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 1501 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 1561 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 1621 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 1681 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 1741 ggttttgcgc cattcgatgg tgtcggatcg agatcgatct cgatcccgcg aaattaatac 1801 gactcactat aggggaattg tgagcggata acaattccta ctagagatta aagaggagaa 1861 aaactacatg gaaaacctgt acttccagtt tcgtagcaaa ggagaagaac ttgtcactgg 1921 agttagcaaa ggagaagaac ttttcactgg agttgtgcca attcttgttg aattagatgg 1981 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg ctacatacgg 2041 aaaactcacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact 2101 tgtcactact ctgacctatg gcgttcaatg cttttcccgt tatccggatc acatgaaacg 2161 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt 2221 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt 2281 taatcgtatc gagttaaagg gtattgattt taaagaagat ggaaacattc tcggacacaa 2341 actagagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg 2401 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggttccgttc aactagcaga 2461 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta 2521 cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct 2581 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaatgacc 2641 aattattgaa ggccgctaac gcggcctttt tttgtttctg gtctcccaaa aatttatttg 2701 cttattaatc atccggctcg tataatgtgt ggagggccca agttcactta aaaaggagaa 2761 tagttatgaa atcgaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg 2821 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg 2881 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat 2941 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt 3001 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggattc 3061 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct 3121 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt 3181 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctccc 3241 tgcacgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct 3301 tcctaatgca ggagtcgcat aagggagagc gccgtccgat gcccttgaga gccttcaacc 3361 cagtcagctc cttccggtgg gcgcggggca tgaccattgt ggccgcactt atgactgtct 3421 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg 3481 accgctttcg ctggagcgcg acgatgagcg gcctgtcgct tgcggtattc ggaatcttgc 3541 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg 3601 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc 3661 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg 3721 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat 3781 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt 3841 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc 3901 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgagaca 3961 attgtccaga taaaaaaaat ccttagcttt cgctaaggat gatttctaaa atttttctgt 4021 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt aaatcagact 4081 gaagacttta tctctctgtc ataaaactgt catattcctt acatataact gtcacctgtt 4141 tgtcctattt tgcttctcgt agccaacaaa caatgcttta tgaatcctcc caggagacat 4201 tatgaaagtt atgcgtacca ccgtcgcaac tgttgtcgcc gcgaccttat cgatgagtgc 4261 tttctctgtg tttgcagaag caagcctgac aggtgcaggt gcaaccttcc ctgcgccggt 4321 gtatgccaaa tgggctgaca cttaccagaa agaaaccggt aataaagtta actaccaggg 4381 tatcggttct tccggtggcg taaaacagat tatcgctaat accgttgatt ttggtgcctc 4441 tgacgcgcc //