LOCUS SEGA072 4746 bp ds-DNA linear UNA 08-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA072 VERSION SEGA072.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(604..1686) /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label="lacI" /codon_start=1 /gene="lacI" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalacto pyranoside (IPTG)." /product="lac repressor" promoter complement(1687..1764) /label="lacI promoter" /gene="lacI" /note="lacI promoter" promoter 1795..1813 /label="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" /note="T7 promoter" protein_bind 1814..1838 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalacto pyranoside (IPTG)." /featureType="protein_bind" RBS 1850..1876 /note="medium TIR" /note="TIR" CDS 1868..1930 /protein_id="NP_416361.2" /translation="MKKRGAFLGLLLVSACASVFA" /label="yebF" /gene_synonym="ECK1848; JW1836" /codon_start=1 /db_xref="GI:145698278" /db_xref="GeneID:946363" /db_xref="ASAP:ABE-0006141" /db_xref="EcoGene:EG11807" /db_xref="UniProtKB/Swiss-Prot:P33219" /gene="yebF" /note="yebFss" /product="yebF signal sequence" /locus_tag="b1847" CDS 1931..2221 /translation="ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKI VGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR" /label="YebF" /codon_start=1 /note="YebF" /product="full size yebF protein" CDS 2222..2932 /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGI KANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="sfGFP" /codon_start=1 /note="sfGFP" /product="superfolder GFP" terminator 2936..2984 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 2995..3023 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 3063..4253 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="confers resistance to tetracycline" /note="TcR" /product="tetracycline efflux protein" terminator 4264..4304 /label="rrnC" /note="rrnC" CDS 4499..4746 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 721 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 781 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 841 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 901 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 961 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 1021 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 1081 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 1141 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 1201 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 1261 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 1321 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 1381 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 1441 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 1501 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 1561 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 1621 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 1681 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 1741 ggttttgcgc cattcgatgg tgtcggatcg agatcgatct cgatcccgcg aaattaatac 1801 gactcactat aggggaattg tgagcggata acaattccta ctagagatta aagaggagaa 1861 agactggatg aaaaagagag gggcgttttt agggctgttg ttggtttctg cctgcgcatc 1921 agttttcgct gcgaataacg aaacgagcaa gagcgtcacc ttcccgaagt gcgaggatct 1981 ggacgcagcg ggcattgctg ccagcgtgaa acgtgactac caacagaacc gcgtggctcg 2041 ttgggcagac gaccagaaga tcgtcggtca agcggacccg gttgcctggg tttccctgca 2101 agatatccag ggcaaagatg ataaatggag cgtcccgctg accgttcgtg gtaagagcgc 2161 ggacattcac tatcaggtga gcgtcgattg caaagccggc atggcggagt accaacgccg 2221 ccgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac tggatggtga 2281 tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa 2341 actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc cgactctggt 2401 aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccaca tgaagcagca 2461 tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga tttcctttaa 2521 ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa 2581 ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg gccataagct 2641 ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa aaaatggcat 2701 taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc tggctgatca 2761 ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca atcactatct 2821 gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcaca tggttctgct 2881 ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca aatgaccaat 2941 tattgaaggc cgctaacgcg gccttttttt gtttctggtc tcccaaaaat ttatttgctt 3001 attaatcatc cggctcgtat aatgtgtgga gggcccaagt tcacttaaaa aggagaatag 3061 ttatgaaatc gaacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag 3121 gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca 3181 gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg 3241 cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc 3301 tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg tggattctct 3361 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata 3421 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt 3481 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccctgc 3541 acgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc 3601 taatgcagga gtcgcataag ggagagcgcc gtccgatgcc cttgagagcc ttcaacccag 3661 tcagctcctt ccggtgggcg cggggcatga ccattgtggc cgcacttatg actgtcttct 3721 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc 3781 gctttcgctg gagcgcgacg atgagcggcc tgtcgcttgc ggtattcgga atcttgcacg 3841 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca 3901 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag 3961 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt 4021 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc 4081 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg 4141 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg 4201 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgagacaatt 4261 gtccagataa aaaaaatcct tagctttcgc taaggatgat ttctaaaatt tttctgtcat 4321 ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttaaa tcagactgaa 4381 gactttatct ctctgtcata aaactgtcat attccttaca tataactgtc acctgtttgt 4441 cctattttgc ttctcgtagc caacaaacaa tgctttatga atcctcccag gagacattat 4501 gaaagttatg cgtaccaccg tcgcaactgt tgtcgccgcg accttatcga tgagtgcttt 4561 ctctgtgttt gcagaagcaa gcctgacagg tgcaggtgca accttccctg cgccggtgta 4621 tgccaaatgg gctgacactt accagaaaga aaccggtaat aaagttaact accagggtat 4681 cggttcttcc ggtggcgtaa aacagattat cgctaatacc gttgattttg gtgcctctga 4741 cgcgcc //