LOCUS SEGA074 4423 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA074 VERSION SEGA074.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(611..1513) /label="NahR-AM" /note="NahR-AM" RBS 1514..1542 /label="nah2" /note="nah2" promoter complement(1543..1596) /label="pLacIQ" /note="lacI promoter" /note="pLacIQ" promoter 1623..1738 /label="pSalTTC" /note="pSalTTC" misc_feature 1739..1813 /label="RiboJ" /note="RiboJ" /note="insulator: self-cleaving ribozyme RiboJ" RBS 1825..1851 /note="TIR" /note="low TIR" CDS 1843..1898 /translation="MKAIFVLKHLNHAKEVF" /label="BCD" /codon_start=1 /note="BCD" CDS 1899..2609 /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGI KANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="GFP" /codon_start=1 /note="GFP" /product="green fluorescent protein" terminator 2613..2661 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator (synthetic)" promoter 2672..2700 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 2740..3930 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="confers resistance to tetracycline" /note="TcR" /product="tetracycline efflux protein" terminator 3941..3981 /label="rrnC" /note="rrnC" CDS 4176..4423 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccccaatt 601 aggatcctta tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta 661 ggtccttgtg gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg 721 ggtgcggcaa ggcgcttagg ccgaagggct ctacgcagca gtcggctaaa catatcggca 781 cagtggcgag cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt 841 ccagacggat gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc 901 cggtgccagc ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg 961 gttcgcgggt gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc 1021 ggcgctgaaa gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc 1081 cgttctgcaa ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat 1141 tgggggcctg gtgagccagc gcatccatca gccgcggcat gaagtagatc tcgccaatgt 1201 cggtcatggc cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct 1261 gtagggcgtt gcgcagtgcg tgcatggccg aagtgacgtg ctcggccaga tgcgcggcat 1321 agggtgtggg ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc 1381 gcaggcgttt cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga 1441 cagagacgcg tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt 1501 cacgcagttc catattcaac ccccatgaat tgactctctt ccgggcgcta tcatgccata 1561 ccgcgaaagg ttttgcacca ttcgatggcg cgccgccatt ggagcttaac gatcgttggc 1621 tgggggcctc gcttgggtta ttgctggtgc ccggccgggc gcaatattca tgttgatgat 1681 ttattatata tcgagtggtg tatttattta tattgtttgc tccgttaccg ttattaacag 1741 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa 1801 taattttgtt taatactaga gattaaagag gagaaagggg ggatgaaggc tattttcgta 1861 ctgaaacatc ttaatcatgc taaggaggtt ttctaatgag caaaggagaa gaacttttca 1921 ctggagttgt gccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg 1981 tcagtggaga gggtgaaggt gatgctacat acggaaaact cacccttaaa tttatttgca 2041 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactctgacc tatggcgttc 2101 aatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc 2161 ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggaac tacaagacgc 2221 gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg 2281 attttaaaga agatggaaac attctcggac acaaactaga gtacaactat aactcacaca 2341 atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc 2401 acaacattga agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg 2461 gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct gccctttcga 2521 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga 2581 ttacacatgg catggatgag ctctacaaat gaccaattat tgaaggccgc taacgcggcc 2641 tttttttgtt tctggtctcc caaaaattta tttgcttatt aatcatccgg ctcgtataat 2701 gtgtggaggg cccaagttca cttaaaaagg agaatagtta tgaaatcgaa caatgcgctc 2761 atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta 2821 ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg 2881 ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc 2941 gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac 3001 gcgatcatgg cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggccggc 3061 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 3121 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 3181 cccgtggccg ggggactgtt gggcgccatc tccctgcacg caccattcct tgcggcggcg 3241 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 3301 gagcgccgtc cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg 3361 ggcatgacca ttgtggccgc acttatgact gtcttcttta tcatgcaact cgtaggacag 3421 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg 3481 agcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact 3541 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac 3601 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg 3661 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag 3721 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact 3781 tcgatcactg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac 3841 gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc 3901 ggtgcatgga gccgggccac ctcgacctga gacaattgtc cagataaaaa aaatccttag 3961 ctttcgctaa ggatgatttc taaaattttt ctgtcatctc ttcgttatta atgtttgtaa 4021 ttgactgaat atcaacgctt atttaaatca gactgaagac tttatctctc tgtcataaaa 4081 ctgtcatatt ccttacatat aactgtcacc tgtttgtcct attttgcttc tcgtagccaa 4141 caaacaatgc tttatgaatc ctcccaggag acattatgaa agttatgcgt accaccgtcg 4201 caactgttgt cgccgcgacc ttatcgatga gtgctttctc tgtgtttgca gaagcaagcc 4261 tgacaggtgc aggtgcaacc ttccctgcgc cggtgtatgc caaatgggct gacacttacc 4321 agaaagaaac cggtaataaa gttaactacc agggtatcgg ttcttccggt ggcgtaaaac 4381 agattatcgc taataccgtt gattttggtg cctctgacgc gcc //