##gff-version 3 ##sequence-region SEGA076 1 4103 ##sequence-region SEGA021 1 4458 ##sequence-region SEGA022 1 4458 SEGA076 . CDS 1 528 . + 0 ID=annotation0;Name=glmS SEGA076 . terminator 3621 3661 . + 0 ID=annotation13;Name=rrnC SEGA076 . CDS 1523 1578 . + 0 ID=annotation8;Name=BCD SEGA076 . CDS 3856 4103 . + 0 ID=annotation14;Name=pstS SEGA076 . terminator 2293 2341 . + 0 ID=annotation10;Name=L3S3P22 Terminator (synthetic) SEGA076 . promoter 1263 1316 . - 0 ID=annotation4;Name=pLacI SEGA076 . biological_region 611 1234 . - 0 ID=annotation2;Name=TetR SEGA076 . terminator 538 594 . + 0 ID=annotation1;Name=L3S2P11 Terminator SEGA076 . ribosome_entry_site 1235 1262 . + 0 ID=annotation3;Name=tet1 SEGA076 . biological_region 1419 1493 . + 0 ID=annotation6;Name=RiboJ SEGA076 . promoter 1343 1418 . + 0 ID=annotation5;Name=pTet* SEGA076 . CDS 2420 3610 . + 0 ID=annotation12;Name=TcR SEGA076 . ribosome_entry_site 1505 1531 . + 0 ID=annotation7;Name=low TIR SEGA076 . promoter 2352 2380 . + 0 ID=annotation11;Name=Promoter 3BCD2 SEGA076 . CDS 1579 2289 . + 0 ID=annotation9;Name=GFP SEGA021 . CDS 4211 4458 . + 0 ID=annotation13;Name=pstS SEGA021 . terminator 3976 4016 . + 0 ID=annotation12;Name=rrnC SEGA021 . CDS 1878 1933 . + 0 ID=annotation7;Name=BCD SEGA021 . ribosome_entry_site 1860 1886 . + 0 ID=annotation6;Name=medium TIR SEGA021 . terminator 2648 2696 . + 0 ID=annotation9;Name=L3S3P22 Terminator (synthetic) SEGA021 . promoter 1795 1824 . + 0 ID=annotation4;Name=trc promoter SEGA021 . promoter 2707 2735 . + 0 ID=annotation10;Name=Promoter 3BCD2 SEGA021 . CDS 1 528 . + 0 ID=annotation0;Name=glmS SEGA021 . sequence_feature 1825 1848 . + 0 ID=annotation5;Name=lacO SEGA021 . promoter 1687 1764 . - 0 ID=annotation3;Name=lacI promoter SEGA021 . CDS 1934 2644 . + 0 ID=annotation8;Name=GFP SEGA021 . CDS 2775 3965 . + 0 ID=annotation11;Name=TcR SEGA021 . terminator 538 594 . + 0 ID=annotation1;Name=L3S2P11 Terminator SEGA021 . CDS 604 1686 . - 0 ID=annotation2;Name=lacI SEGA022 . CDS 1 528 . + 0 ID=annotation0;Name=glmS SEGA022 . CDS 604 1686 . - 0 ID=annotation2;Name=lacI SEGA022 . CDS 1878 1933 . + 0 ID=annotation7;Name=BCD SEGA022 . ribosome_entry_site 1860 1886 . + 0 ID=annotation6;Name=high TIR SEGA022 . promoter 2707 2735 . + 0 ID=annotation10;Name=Promoter 3BCD2 SEGA022 . terminator 3976 4016 . + 0 ID=annotation12;Name=rrnC SEGA022 . CDS 4211 4458 . + 0 ID=annotation13;Name=pstS SEGA022 . sequence_feature 1825 1848 . + 0 ID=annotation5;Name=lacO SEGA022 . CDS 2775 3965 . + 0 ID=annotation11;Name=TcR SEGA022 . terminator 538 594 . + 0 ID=annotation1;Name=L3S2P11 Terminator SEGA022 . promoter 1795 1824 . + 0 ID=annotation4;Name=trc promoter SEGA022 . promoter 1687 1764 . - 0 ID=annotation3;Name=lacI promoter SEGA022 . terminator 2648 2696 . + 0 ID=annotation9;Name=L3S3P22 Terminator (synthetic) SEGA022 . CDS 1934 2644 . + 0 ID=annotation8;Name=GFP >SEGA076 gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaag atttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagtt gaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgcc gatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgc gtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgcc gcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaa ggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattcc agaaaagagacgctttcgagcgtcttttttcgttttggtccccaattaggatccttatcaggacccactttcacattta agttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataatt cgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttg atcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgt tggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctc catcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaa agggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgc caatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaa gcagctctaatgcgctgttaatcactttacttttatctaatctggacatattcaccaccctgaattgactctcttccgg gcgctatcatgccataccgcgaaaggttttgcgccattcgatggcgcgccgccattggagcttaacgatcgttggctgt tttcagcaggacgcactgacctccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacagct gtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaatactagag attaaagaggagaaaggggggatgaaggctattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgag caaaggagaagaacttttcactggagttgtgccaattcttgttgaattagatggtgatgttaatgggcacaaattttct gtcagtggagagggtgaaggtgatgctacatacggaaaactcacccttaaatttatttgcactactggaaaactacctg ttccatggccaacacttgtcactactctgacctatggcgttcaatgcttttcccgttatccggatcacatgaaacggca tgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaag acgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatg gaaacattctcggacacaaactagagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatgg aatcaaagctaacttcaaaattcgccacaacattgaagatggttccgttcaactagcagaccattatcaacaaaatact ccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacg aaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatg accaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaaaaatttatttgcttattaatcatccgg ctcgtataatgtgtggagggcccaagttcacttaaaaaggagaatagttatgaaatcgaacaatgcgctcatcgtcatc ctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtcc attccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttct cggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatc atggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttg ctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgt gggtatggtggcaggccccgtggccgggggactgttgggcgccatctccctgcacgcaccattccttgcggcggcggtg ctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgccgtccgatgcccttga gagccttcaacccagtcagctccttccggtgggcgcggggcatgaccattgtggccgcacttatgactgtcttctttat catgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatg agcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtt tcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgagg ctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccagg caggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgc tgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctatacct tgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgagacaattgtccagataaaaaaaat ccttagctttcgctaaggatgatttctaaaatttttctgtcatctcttcgttattaatgtttgtaattgactgaatatc aacgcttatttaaatcagactgaagactttatctctctgtcataaaactgtcatattccttacatataactgtcacctg tttgtcctattttgcttctcgtagccaacaaacaatgctttatgaatcctcccaggagacattatgaaagttatgcgta ccaccgtcgcaactgttgtcgccgcgaccttatcgatgagtgctttctctgtgtttgcagaagcaagcctgacaggtgc aggtgcaaccttccctgcgccggtgtatgccaaatgggctgacacttaccagaaagaaaccggtaataaagttaactac cagggtatcggttcttccggtggcgtaaaacagattatcgctaataccgttgattttggtgcctctgacgcgcc >SEGA021 gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaag atttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagtt gaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgcc gatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgc gtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgcc gcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaa ggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattcc agaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacct gtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttct tttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctg gtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgt atcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatc gttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactc cagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcg ccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcg cgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacatta gtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcga gaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttg atcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagc aacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttttt cccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgc gacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcga aaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcg tataatggaattgtgagcggataacaattcctactagagattaaagaggagaaagagctaatgaaggctattttcgtac tgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattctt gttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaac tcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgt tcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacag gaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgtta atcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactc acacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagat ggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccatt acctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgc tgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgttt ctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaagg agaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttgg ttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagc gctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctg ctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggac gcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcg ggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggc gccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgc aggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcgggg catgaccattgtggccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtc attttcggcgaggaccgctttcgctggagcgcgacgatgagcggcctgtcgcttgcggtattcggaatcttgcacgccc tcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccga cgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggc ggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgc tcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatg gaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgg gccacctcgacctgagacaattgtccagataaaaaaaatccttagctttcgctaaggatgatttctaaaatttttctgt catctcttcgttattaatgtttgtaattgactgaatatcaacgcttatttaaatcagactgaagactttatctctctgt cataaaactgtcatattccttacatataactgtcacctgtttgtcctattttgcttctcgtagccaacaaacaatgctt tatgaatcctcccaggagacattatgaaagttatgcgtaccaccgtcgcaactgttgtcgccgcgaccttatcgatgag tgctttctctgtgtttgcagaagcaagcctgacaggtgcaggtgcaaccttccctgcgccggtgtatgccaaatgggct gacacttaccagaaagaaaccggtaataaagttaactaccagggtatcggttcttccggtggcgtaaaacagattatcg ctaataccgttgattttggtgcctctgacgcgcc >SEGA022 gtgcatggtctgcaggcgctgccgagccgtattgagcagatgctgtctcaggacaaacgcattgaagcgctggcagaag atttctctgacaaacatcacgcgctgttcctgggccgtggcgatcagtacccaatcgcgctggaaggcgcattgaagtt gaaagagatctcttacattcacgctgaagcctacgctgctggcgaactgaaacacggtccgctggcgctaattgatgcc gatatgccggttattgttgttgcaccgaacaacgaattgctggaaaaactgaaatccaacattgaagaagttcgcgcgc gtggcggtcagttgtatgtcttcgccgatcaggatgcgggttttgtaagtagcgataacatgcacatcatcgagatgcc gcatgtggaagaggtgattgcaccgatcttctacaccgttccgctgcagctgctggcttaccatgtcgcgctgatcaaa ggcaccgacgttgaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatctcggtaccaaattcc agaaaagagacgctttcgagcgtcttttttcgttttggtccgcgttgcgctcactgcccgctttccagtcgggaaacct gtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttct tttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctg gtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgt atcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatc gttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactc cagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcg ccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcg cgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacatta gtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcga gaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttg atcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagc aacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttttt cccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgc gacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcga aaggttttgcgccattcgatggtgtcggatcgagatcgatctcgatcccgcgaaatttgacaattaatcatccggctcg tataatggaattgtgagcggataacaattcctactagagattaaagaggagaaactatatatgaaagcaattttcgtac tgaaacatcttaatcatgctaaggaggttttctaatgagcaaaggagaagaacttttcactggagttgtgccaattctt gttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaaac tcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggcgt tcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacag gaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgtta atcgtatcgagttaaagggtattgattttaaagaagatggaaacattctcggacacaaactagagtacaactataactc acacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagat ggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccatt acctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgc tgctgggattacacatggcatggatgagctctacaaatgaccaattattgaaggccgctaacgcggcctttttttgttt ctggtctcccaaaaatttatttgcttattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaagg agaatagttatgaaatcgaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttgg ttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagc gctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctg ctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggac gcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcg ggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggc gccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgc aggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcgggg catgaccattgtggccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtc attttcggcgaggaccgctttcgctggagcgcgacgatgagcggcctgtcgcttgcggtattcggaatcttgcacgccc tcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccga cgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggc ggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgc tcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatg gaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgg gccacctcgacctgagacaattgtccagataaaaaaaatccttagctttcgctaaggatgatttctaaaatttttctgt catctcttcgttattaatgtttgtaattgactgaatatcaacgcttatttaaatcagactgaagactttatctctctgt cataaaactgtcatattccttacatataactgtcacctgtttgtcctattttgcttctcgtagccaacaaacaatgctt tatgaatcctcccaggagacattatgaaagttatgcgtaccaccgtcgcaactgttgtcgccgcgaccttatcgatgag tgctttctctgtgtttgcagaagcaagcctgacaggtgcaggtgcaaccttccctgcgccggtgtatgccaaatgggct gacacttaccagaaagaaaccggtaataaagttaactaccagggtatcggttcttccggtggcgtaaaacagattatcg ctaataccgttgattttggtgcctctgacgcgcc