LOCUS SEGA022 4458 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA022 VERSION SEGA022.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 4458) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(604..1686) /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label="lacI" /codon_start=1 /gene="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalacto pyranoside (IPTG)." /note="lacI" /product="lac repressor" promoter complement(1687..1764) /label="lacI promoter" /gene="lacI" /note="lacI promoter" promoter 1795..1824 /label="trc promoter" /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" /note="trc promoter" protein_bind 1825..1848 /bound_moiety="lac repressor" /label="lacO" /note="lac operator" /note="lacO" /featureType="protein_bind" RBS 1860..1886 /note="TIR" /note="high TIR" CDS 1878..1933 /translation="MKAIFVLKHLNHAKEVF" /label="BCD" /codon_start=1 /note="BCD" CDS 1934..2644 /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGI KANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="GFP" /codon_start=1 /note="GFP" /product="green fluorescent protein" terminator 2648..2696 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 2707..2735 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 2775..3965 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="confers resistance to tetracycline" /note="TcR" /product="tetracycline efflux protein" terminator 3976..4016 /label="rrnC" /note="rrnC" CDS 4211..4458 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 721 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 781 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 841 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 901 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 961 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 1021 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 1081 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 1141 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 1201 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 1261 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 1321 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 1381 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 1441 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 1501 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 1561 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 1621 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 1681 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 1741 ggttttgcgc cattcgatgg tgtcggatcg agatcgatct cgatcccgcg aaatttgaca 1801 attaatcatc cggctcgtat aatggaattg tgagcggata acaattccta ctagagatta 1861 aagaggagaa actatatatg aaagcaattt tcgtactgaa acatcttaat catgctaagg 1921 aggttttcta atgagcaaag gagaagaact tttcactgga gttgtgccaa ttcttgttga 1981 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 2041 tacatacgga aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 2101 gccaacactt gtcactactc tgacctatgg cgttcaatgc ttttcccgtt atccggatca 2161 catgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac 2221 tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga 2281 tacccttgtt aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct 2341 cggacacaaa ctagagtaca actataactc acacaatgta tacatcacgg cagacaaaca 2401 aaagaatgga atcaaagcta acttcaaaat tcgccacaac attgaagatg gttccgttca 2461 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 2521 caaccattac ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca 2581 catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta 2641 caaatgacca attattgaag gccgctaacg cggccttttt ttgtttctgg tctcccaaaa 2701 atttatttgc ttattaatca tccggctcgt ataatgtgtg gagggcccaa gttcacttaa 2761 aaaggagaat agttatgaaa tcgaacaatg cgctcatcgt catcctcggc accgtcaccc 2821 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg 2881 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc 2941 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc 3001 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc 3061 tgtggattct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 3121 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 3181 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 3241 ccatctccct gcacgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 3301 tgggctgctt cctaatgcag gagtcgcata agggagagcg ccgtccgatg cccttgagag 3361 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gaccattgtg gccgcactta 3421 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt 3481 tcggcgagga ccgctttcgc tggagcgcga cgatgagcgg cctgtcgctt gcggtattcg 3541 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg 3601 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt 3661 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg 3721 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc 3781 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca 3841 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg 3901 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga 3961 cctgagacaa ttgtccagat aaaaaaaatc cttagctttc gctaaggatg atttctaaaa 4021 tttttctgtc atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttattta 4081 aatcagactg aagactttat ctctctgtca taaaactgtc atattcctta catataactg 4141 tcacctgttt gtcctatttt gcttctcgta gccaacaaac aatgctttat gaatcctccc 4201 aggagacatt atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg cgaccttatc 4261 gatgagtgct ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg caaccttccc 4321 tgcgccggtg tatgccaaat gggctgacac ttaccagaaa gaaaccggta ataaagttaa 4381 ctaccagggt atcggttctt ccggtggcgt aaaacagatt atcgctaata ccgttgattt 4441 tggtgcctct gacgcgcc // LOCUS SEGA078 6528 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA078 VERSION SEGA078.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 4458) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(605..1753) /protein_id="NP_415278.1" /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETLYVCKPSQGAGQC" /function="enzyme; Degradation of small molecules: Carbon compounds" /label="galK" /gene_synonym="ECK0746; galA; JW0740" /EC_number="2.7.1.6" /codon_start=1 /db_xref="GI:16128725" /db_xref="EcoGene:EG10363" /db_xref="ASAP:ABE-0002568" /db_xref="GeneID:945358" /db_xref="UniProtKB/Swiss-Prot:P0A6T3" /gene="galK" /note="galK" /product="galactokinase" /locus_tag="b0757" promoter complement(1783..1830) /label="EM7 promoter" /note="synthetic bacterial promoter" /note="EM7 promoter" terminator 1843..1886 /label="Terminator rrnB T1" /label="rrnB" /note="rrnB" CDS complement(1899..2744) /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG AIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH LNSQKD" /label="rhaR" /codon_start=1 /gene="E. coli rhaR" /note="rhaR" /note="needed for rhamnose-dependent activation of the rhaB promoter" /product="transcriptional activator of rhaR and rhaS" CDS complement(2818..3654) /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF LQ" /label="rhaS" /codon_start=1 /gene="E. coli rhaS" /note="activated by L-rhamnose" /note="rhaS" /product="positive regulator of the rhaB promoter" promoter 3800..3918 /label="rhaB promoter" /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /note="rhaB promoter" RBS 3930..3956 /note="TIR" /note="low TIR" CDS 3948..4003 /translation="MKAIFVLKHLNHAKEVF" /label="BCD" /codon_start=1 /note="BCD" CDS 4004..4714 /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGI KANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="GFP" /codon_start=1 /note="GFP" /product="green fluorescent protein" terminator 4718..4766 /label="L3S3P22" /note="L3S3P22" promoter 4777..4805 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 4845..6035 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="TcR" /note="confers resistance to tetracycline" /product="tetracycline efflux protein" terminator 6046..6086 /label="rrnC" /note="rrnC" CDS 6281..6528 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccaccgta 601 ttcatcagca ctgtcctgct ccttgtgatg gtttacaaac gtaaagagtc tctttaatac 661 ctgtttttgc ttcatattgt tcagcgacag cttgctgtac ggcaggcacc agctcttccg 721 ggatcagcgc gacgatacag ccgccaaatc cgccgccggt catgcgtacg ccacctttgt 781 cgccaatcac agctttgacg atttctacca gagtgtcaat ttgcggcacg gtgatttcga 841 aatcatcgcg catagaggca tgagactccg ccatcaactc gcccatacgt ttcaggtcgc 901 cttgctccag cgcgctggca gcttcaacgg tgcgggcgtt ttcagtcagt atatgacgca 961 cgcgttttgc cacgatcggg tccagttcat gcgcaacagc gttgaactct tcaatggtga 1021 catcacgcag ggctggctgc tggaagaaac gcgcaccggt ttcgcactgt tcacgacggg 1081 tgttgtattc gctgccaacc agggtacgtt tgaagttact gttgatgatg acgacagcca 1141 cacctttggg catggaaact gctttggtcc ccagtgagcg gcaatcgatc agcaaggcat 1201 gatctttctt gccgagcgcg gaaattagct gatccatgat cccgcagtta cagcctacaa 1261 actggttttc tgcttcctga ccgttaagcg cgatttgtgc gccgtccagc ggcagatgat 1321 aaagctgctg caatacggtt ccgaccgcga cttccagtga agcggaagaa cttaacccgg 1381 caccctgcgg cacattgccg ctgatcacca tgtccacgcc gccgaagctg ttgttacgca 1441 gttgcagatg tttcaccacg ccacgaacgt agttagccca ttgatagttt tcatgtgcga 1501 caatgggcgc atcgagggaa aactcgtcga gctgattttc ataatcggct gccatcacgc 1561 gaactttacg gtcatcgcgt ggtgcacaac tgatcacggt ttgataatca atcgcgcagg 1621 gcagaacgaa accgtcgttg tagtcggtgt gttcaccaat caaattcacg cggccaggcg 1681 cctgaatggt gtgagtggca gggtagccaa atgcgttggc aaacagagat tgtgtttttt 1741 ctttcagact catgattgcc tcctgggtta gttcctcacc ttgtcgtatt atactatgcc 1801 gatatactat gccgatgatt aattgtcaac ctttaatgaa ttataaaacg aaaggctcag 1861 tcgaaagact gggcctttcg ttttatgcgt tgcgcttaat ctttctgcga attgagatga 1921 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta 1981 tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga 2041 tattgcgcat gacacactct gacctgtcgc agatattgat tgatggtcat tccagtctgc 2101 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc 2161 agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc cagcaacgtt 2221 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc 2281 aactgcccga acagcaactc agccatttcg ttagcaaacg gcacatgctg actactttca 2341 tgctcaagct gaccaataac ctgccgcgcc tgcgccatcc ccatgctacc taagcgccag 2401 tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc cctgccagtc aagattcagc 2461 ttcagacgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag 2521 gagtgtttat catcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga 2581 tcgttgagta catgcaggcc attaccgcgc cagacaatca ccagctcaca aaaatcatgt 2641 gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg 2701 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc 2761 agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta 2821 ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt gaccagttaa actctcggcg 2881 aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag cgataggcga tgtcagtaac 2941 gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc ggttcaggta 3001 tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag 3061 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca 3121 ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt 3181 acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc 3241 attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct 3301 gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt 3361 cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag 3421 attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc 3481 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac 3541 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat 3601 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc 3661 ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt 3721 ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga 3781 aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct 3841 ttccctggtt gccaatggcc cattttcttg tcagtaacga gaaggtcgcg aatccaggcg 3901 ctttttagac tggtcgtata ctagagatta aagaggagaa aggggggatg aaggctattt 3961 tcgtactgaa acatcttaat catgctaagg aggttttcta atgagcaaag gagaagaact 4021 tttcactgga gttgtgccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt 4081 ttctgtcagt ggagagggtg aaggtgatgc tacatacgga aaactcaccc ttaaatttat 4141 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactc tgacctatgg 4201 cgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc 4261 catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg ggaactacaa 4321 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg 4381 tattgatttt aaagaagatg gaaacattct cggacacaaa ctagagtaca actataactc 4441 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat 4501 tcgccacaac attgaagatg gttccgttca actagcagac cattatcaac aaaatactcc 4561 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgccct 4621 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc 4681 tgggattaca catggcatgg atgagctcta caaatgacca attattgaag gccgctaacg 4741 cggccttttt ttgtttctgg tctcccaaaa atttatttgc ttattaatca tccggctcgt 4801 ataatgtgtg gagggcccaa gttcacttaa aaaggagaat agttatgaaa tcgaacaatg 4861 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc 4921 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg 4981 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac 5041 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg 5101 actacgcgat catggcgacc acacccgtcc tgtggattct ctacgccgga cgcatcgtgg 5161 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg 5221 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg 5281 caggccccgt ggccggggga ctgttgggcg ccatctccct gcacgcacca ttccttgcgg 5341 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata 5401 agggagagcg ccgtccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg 5461 cgcggggcat gaccattgtg gccgcactta tgactgtctt ctttatcatg caactcgtag 5521 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga 5581 cgatgagcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg 5641 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg 5701 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca 5761 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca 5821 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc 5881 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat 5941 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc 6001 gtcgcggtgc atggagccgg gccacctcga cctgagacaa ttgtccagat aaaaaaaatc 6061 cttagctttc gctaaggatg atttctaaaa tttttctgtc atctcttcgt tattaatgtt 6121 tgtaattgac tgaatatcaa cgcttattta aatcagactg aagactttat ctctctgtca 6181 taaaactgtc atattcctta catataactg tcacctgttt gtcctatttt gcttctcgta 6241 gccaacaaac aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac 6301 cgtcgcaact gttgtcgccg cgaccttatc gatgagtgct ttctctgtgt ttgcagaagc 6361 aagcctgaca ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac 6421 ttaccagaaa gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt 6481 aaaacagatt atcgctaata ccgttgattt tggtgcctct gacgcgcc // LOCUS SEGA021 4458 bp ds-DNA linear UNA 08-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA021 VERSION SEGA021.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 4458) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(604..1686) /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" /label="lacI" /codon_start=1 /gene="lacI" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalacto pyranoside (IPTG)." /product="lac repressor" promoter complement(1687..1764) /label="lacI promoter" /gene="lacI" /note="lacI promoter" promoter 1795..1824 /label="trc promoter" /note="trc promoter" /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 1825..1848 /bound_moiety="lac repressor" /label="lacO" /note="lac operator" /note="lacO" /featureType="protein_bind" RBS 1860..1886 /note="medium TIR" /note="TIR" CDS 1878..1933 /translation="MKAIFVLKHLNHAKEVF" /label="BCD" /codon_start=1 /note="BCD" CDS 1934..2644 /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGI KANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="GFP" /codon_start=1 /note="GFP" /product="green fluorescent protein" terminator 2648..2696 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 2707..2735 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 2775..3965 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="TcR" /note="confers resistance to tetracycline" /product="tetracycline efflux protein" terminator 3976..4016 /label="rrnC" /note="rrnC" CDS 4211..4458 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 721 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 781 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 841 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 901 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 961 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 1021 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 1081 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 1141 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 1201 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 1261 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 1321 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 1381 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 1441 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 1501 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 1561 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 1621 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 1681 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 1741 ggttttgcgc cattcgatgg tgtcggatcg agatcgatct cgatcccgcg aaatttgaca 1801 attaatcatc cggctcgtat aatggaattg tgagcggata acaattccta ctagagatta 1861 aagaggagaa agagctaatg aaggctattt tcgtactgaa acatcttaat catgctaagg 1921 aggttttcta atgagcaaag gagaagaact tttcactgga gttgtgccaa ttcttgttga 1981 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 2041 tacatacgga aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 2101 gccaacactt gtcactactc tgacctatgg cgttcaatgc ttttcccgtt atccggatca 2161 catgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac 2221 tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga 2281 tacccttgtt aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct 2341 cggacacaaa ctagagtaca actataactc acacaatgta tacatcacgg cagacaaaca 2401 aaagaatgga atcaaagcta acttcaaaat tcgccacaac attgaagatg gttccgttca 2461 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 2521 caaccattac ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca 2581 catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta 2641 caaatgacca attattgaag gccgctaacg cggccttttt ttgtttctgg tctcccaaaa 2701 atttatttgc ttattaatca tccggctcgt ataatgtgtg gagggcccaa gttcacttaa 2761 aaaggagaat agttatgaaa tcgaacaatg cgctcatcgt catcctcggc accgtcaccc 2821 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg 2881 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc 2941 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc 3001 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc 3061 tgtggattct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 3121 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 3181 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 3241 ccatctccct gcacgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 3301 tgggctgctt cctaatgcag gagtcgcata agggagagcg ccgtccgatg cccttgagag 3361 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gaccattgtg gccgcactta 3421 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt 3481 tcggcgagga ccgctttcgc tggagcgcga cgatgagcgg cctgtcgctt gcggtattcg 3541 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg 3601 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt 3661 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg 3721 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc 3781 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca 3841 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg 3901 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga 3961 cctgagacaa ttgtccagat aaaaaaaatc cttagctttc gctaaggatg atttctaaaa 4021 tttttctgtc atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttattta 4081 aatcagactg aagactttat ctctctgtca taaaactgtc atattcctta catataactg 4141 tcacctgttt gtcctatttt gcttctcgta gccaacaaac aatgctttat gaatcctccc 4201 aggagacatt atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg cgaccttatc 4261 gatgagtgct ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg caaccttccc 4321 tgcgccggtg tatgccaaat gggctgacac ttaccagaaa gaaaccggta ataaagttaa 4381 ctaccagggt atcggttctt ccggtggcgt aaaacagatt atcgctaata ccgttgattt 4441 tggtgcctct gacgcgcc //