LOCUS SEGA085 6493 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA085 VERSION SEGA085.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(605..1753) /protein_id="NP_415278.1" /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC" /function="enzyme; Degradation of small molecules: Carbon compounds" /label="galK" /gene_synonym="ECK0746; galA; JW0740" /EC_number="2.7.1.6" /codon_start=1 /db_xref="EcoGene:EG10363" /db_xref="GeneID:945358" /db_xref="UniProtKB/Swiss-Prot:P0A6T3" /db_xref="GI:16128725" /db_xref="ASAP:ABE-0002568" /gene="galK" /note="galK" /product="galactokinase" /locus_tag="b0757" promoter complement(1783..1830) /label="EM7 promoter" /note="synthetic bacterial promoter" /note="EM7 promoter" terminator 1843..1886 /label="Terminator rrnB T1" /note="Terminator rrnB T1" CDS complement(1899..2744) /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG AIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH LNSQKD" /label="rhaR" /codon_start=1 /gene="E. coli rhaR" /note="rhaR" /note="needed for rhamnose-dependent activation of the rhaB promoter" /product="transcriptional activator of rhaR and rhaS" CDS complement(2818..3654) /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF LQ" /label="rhaS" /codon_start=1 /gene="E. coli rhaS" /note="activated by L-rhamnose" /note="rhaS" /product="positive regulator of the rhaB promoter" promoter 3800..3918 /label="rhaB promoter" /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /note="rhaB promoter" RBS 3930..3956 /note="medium TIR" /note="TIR" CDS 3948..3968 /translation="MENLYFQ" /label="TEV site" /codon_start=1 /note="TEV site" /product="tobacco etch virus (TEV) protease recognition and cleavage site" CDS 3969..4679 /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGI KANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="GFP" /codon_start=1 /note="GFP" /product="green fluorescent protein" terminator 4683..4731 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 4742..4770 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 4810..6000 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="TcR" /note="confers resistance to tetracycline" /product="tetracycline efflux protein" terminator 6011..6051 /label="rrnC" /note="rrnC" CDS 6246..6493 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccaccgta 601 ttcatcagca ctgtcctgct ccttgtgatg gtttacaaac gtaaaaagtc tctttaatac 661 ctgtttttgc ttcatattgt tcagcgacag cttgctgtac ggcaggcacc agctcttccg 721 ggatcagcgc gacgatacag ccgccaaatc cgccgccggt catgcgtacg ccacctttgt 781 cgccaatcac agctttgacg atttctacca gagtgtcaat ttgcggcacg gtgatttcga 841 aatcatcgcg catagaggca tgagactccg ccatcaactc gcccatacgt ttcaggtcgc 901 cttgctccag cgcgctggca gcttcaacgg tgcgggcgtt ttcagtcagt atatgacgca 961 cgcgttttgc cacgatcggg tccagttcat gcgcaacagc gttgaactct tcaatggtga 1021 catcacgcag ggctggctgc tggaagaaac gcgcaccggt ttcgcactgt tcacgacggg 1081 tgttgtattc gctgccaacc agggtacgtt tgaagttact gttgatgatg acgacagcca 1141 cacctttggg catggaaact gctttggtcc ccagtgagcg gcaatcgatc agcaaggcat 1201 gatctttctt gccgagcgcg gaaattagct gatccatgat cccgcagtta cagcctacaa 1261 actggttttc tgcttcctga ccgttaagcg cgatttgtgc gccgtccagc ggcagatgat 1321 aaagctgctg caatacggtt ccgaccgcga cttccagtga agcggaagaa cttaacccgg 1381 caccctgcgg cacattgccg ctgatcacca tgtccacgcc gccgaagctg ttgttacgca 1441 gttgcagatg tttcaccacg ccacgaacgt agttagccca ttgatagttt tcatgtgcga 1501 caatgggcgc atcgagggaa aactcgtcga gctgattttc ataatcggct gccatcacgc 1561 gaactttacg gtcatcgcgt ggtgcacaac tgatcacggt ttgataatca atcgcgcagg 1621 gcagaacgaa accgtcgttg tagtcggtgt gttcaccaat caaattcacg cggccaggcg 1681 cctgaatggt gtgagtggca gggtagccaa atgcgttggc aaacagagat tgtgtttttt 1741 ctttcagact catgattgcc tcctgggtta gttcctcacc ttgtcgtatt atactatgcc 1801 gatatactat gccgatgatt aattgtcaac ctttaatgaa ttataaaacg aaaggctcag 1861 tcgaaagact gggcctttcg ttttatgcgt tgcgcttaat ctttctgcga attgagatga 1921 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta 1981 tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga 2041 tattgcgcat gacacactct gacctgtcgc agatattgat tgatggtcat tccagtctgc 2101 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc 2161 agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc cagcaacgtt 2221 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc 2281 aactgcccga acagcaactc agccatttcg ttagcaaacg gcacatgctg actactttca 2341 tgctcaagct gaccaataac ctgccgcgcc tgcgccatcc ccatgctacc taagcgccag 2401 tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc cctgccagtc aagattcagc 2461 ttcagacgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag 2521 gagtgtttat catcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga 2581 tcgttgagta catgcaggcc attaccgcgc cagacaatca ccagctcaca aaaatcatgt 2641 gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg 2701 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc 2761 agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta 2821 ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt gaccagttaa actctcggcg 2881 aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag cgataggcga tgtcagtaac 2941 gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc ggttcaggta 3001 tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag 3061 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca 3121 ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt 3181 acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc 3241 attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct 3301 gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt 3361 cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag 3421 attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc 3481 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac 3541 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat 3601 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc 3661 ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt 3721 ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga 3781 aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct 3841 ttccctggtt gccaatggcc cattttcttg tcagtaacga gaaggtcgcg aatccaggcg 3901 ctttttagac tggtcgtata ctagagatta aagaggagaa aaactacatg gaaaacctgt 3961 acttccagag caaaggagaa gaacttttca ctggagttgt gccaattctt gttgaattag 4021 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgctacat 4081 acggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa 4141 cacttgtcac tactctgacc tatggcgttc aatgcttttc ccgttatccg gatcacatga 4201 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat 4261 ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt caagtttgaa ggtgataccc 4321 ttgttaatcg tatcgagtta aagggtattg attttaaaga agatggaaac attctcggac 4381 acaaactaga gtacaactat aactcacaca atgtatacat cacggcagac aaacaaaaga 4441 atggaatcaa agctaacttc aaaattcgcc acaacattga agatggttcc gttcaactag 4501 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc 4561 attacctgtc gacacaatct gccctttcga aagatcccaa cgaaaagcgt gaccacatgg 4621 tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaat 4681 gaccaattat tgaaggccgc taacgcggcc tttttttgtt tctggtctcc caaaaattta 4741 tttgcttatt aatcatccgg ctcgtataat gtgtggaggg cccaagttca cttaaaaagg 4801 agaatagtta tgaaatcgaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat 4861 gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat 4921 tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt 4981 ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc 5041 gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg 5101 attctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc 5161 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc 5221 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc 5281 tccctgcacg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc 5341 tgcttcctaa tgcaggagtc gcataaggga gagcgccgtc cgatgccctt gagagccttc 5401 aacccagtca gctccttccg gtgggcgcgg ggcatgacca ttgtggccgc acttatgact 5461 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc 5521 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc 5581 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag 5641 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg 5701 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg 5761 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa 5821 ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg 5881 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta 5941 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga 6001 gacaattgtc cagataaaaa aaatccttag ctttcgctaa ggatgatttc taaaattttt 6061 ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttaaatca 6121 gactgaagac tttatctctc tgtcataaaa ctgtcatatt ccttacatat aactgtcacc 6181 tgtttgtcct attttgcttc tcgtagccaa caaacaatgc tttatgaatc ctcccaggag 6241 acattatgaa agttatgcgt accaccgtcg caactgttgt cgccgcgacc ttatcgatga 6301 gtgctttctc tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc 6361 cggtgtatgc caaatgggct gacacttacc agaaagaaac cggtaataaa gttaactacc 6421 agggtatcgg ttcttccggt ggcgtaaaac agattatcgc taataccgtt gattttggtg 6481 cctctgacgc gcc //