LOCUS SEGA090 6538 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA090 VERSION SEGA090.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(605..1753) /protein_id="NP_415278.1" /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC" /function="enzyme; Degradation of small molecules: Carbon compounds" /label="galK" /gene_synonym="ECK0746; galA; JW0740" /EC_number="2.7.1.6" /codon_start=1 /db_xref="GI:16128725" /db_xref="ASAP:ABE-0002568" /db_xref="EcoGene:EG10363" /db_xref="GeneID:945358" /db_xref="UniProtKB/Swiss-Prot:P0A6T3" /gene="galK" /note="galK" /product="galactokinase" /locus_tag="b0757" promoter complement(1783..1830) /label="EM7 promoter" /note="EM7 promoter" /note="synthetic bacterial promoter" terminator 1843..1886 /label="Terminator rrnB T1" /note="Terminator rrnB T1" CDS complement(1899..2744) /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG AIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH LNSQKD" /label="rhaR" /codon_start=1 /gene="E. coli rhaR" /note="needed for rhamnose-dependent activation of the rhaB promoter" /note="rhaR" /product="transcriptional activator of rhaR and rhaS" CDS complement(2818..3654) /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF LQ" /label="rhaS" /codon_start=1 /gene="E. coli rhaS" /note="activated by L-rhamnose" /note="rhaS" /product="positive regulator of the rhaB promoter" promoter 3800..3918 /label="rhaB promoter" /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /note="rhaB promoter" GC_signal 3930..3956 /note="low TIR" /note="TIR" CDS 3948..4013 /translation="MKYLLPTAAAGLLLLAAQPAMA" /label="pelB signal sequence" /codon_start=1 /gene="pelB (fragment)" /note="pelB signal sequence" /product="leader peptide for secretion" CDS 4014..4724 /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGI KANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="sfGFP" /codon_start=1 /note="sfGFP" /product="superfolder GFP" terminator 4728..4776 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 4787..4815 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 4855..6045 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="confers resistance to tetracycline" /note="TcR" /product="tetracycline efflux protein" terminator 6056..6096 /label="rrnC" /note="rrnC" CDS 6291..6538 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccaccgta 601 ttcatcagca ctgtcctgct ccttgtgatg gtttacaaac gtaaaaagtc tctttaatac 661 ctgtttttgc ttcatattgt tcagcgacag cttgctgtac ggcaggcacc agctcttccg 721 ggatcagcgc gacgatacag ccgccaaatc cgccgccggt catgcgtacg ccacctttgt 781 cgccaatcac agctttgacg atttctacca gagtgtcaat ttgcggcacg gtgatttcga 841 aatcatcgcg catagaggca tgagactccg ccatcaactc gcccatacgt ttcaggtcgc 901 cttgctccag cgcgctggca gcttcaacgg tgcgggcgtt ttcagtcagt atatgacgca 961 cgcgttttgc cacgatcggg tccagttcat gcgcaacagc gttgaactct tcaatggtga 1021 catcacgcag ggctggctgc tggaagaaac gcgcaccggt ttcgcactgt tcacgacggg 1081 tgttgtattc gctgccaacc agggtacgtt tgaagttact gttgatgatg acgacagcca 1141 cacctttggg catggaaact gctttggtcc ccagtgagcg gcaatcgatc agcaaggcat 1201 gatctttctt gccgagcgcg gaaattagct gatccatgat cccgcagtta cagcctacaa 1261 actggttttc tgcttcctga ccgttaagcg cgatttgtgc gccgtccagc ggcagatgat 1321 aaagctgctg caatacggtt ccgaccgcga cttccagtga agcggaagaa cttaacccgg 1381 caccctgcgg cacattgccg ctgatcacca tgtccacgcc gccgaagctg ttgttacgca 1441 gttgcagatg tttcaccacg ccacgaacgt agttagccca ttgatagttt tcatgtgcga 1501 caatgggcgc atcgagggaa aactcgtcga gctgattttc ataatcggct gccatcacgc 1561 gaactttacg gtcatcgcgt ggtgcacaac tgatcacggt ttgataatca atcgcgcagg 1621 gcagaacgaa accgtcgttg tagtcggtgt gttcaccaat caaattcacg cggccaggcg 1681 cctgaatggt gtgagtggca gggtagccaa atgcgttggc aaacagagat tgtgtttttt 1741 ctttcagact catgattgcc tcctgggtta gttcctcacc ttgtcgtatt atactatgcc 1801 gatatactat gccgatgatt aattgtcaac ctttaatgaa ttataaaacg aaaggctcag 1861 tcgaaagact gggcctttcg ttttatgcgt tgcgcttaat ctttctgcga attgagatga 1921 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta 1981 tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga 2041 tattgcgcat gacacactct gacctgtcgc agatattgat tgatggtcat tccagtctgc 2101 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc 2161 agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc cagcaacgtt 2221 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc 2281 aactgcccga acagcaactc agccatttcg ttagcaaacg gcacatgctg actactttca 2341 tgctcaagct gaccaataac ctgccgcgcc tgcgccatcc ccatgctacc taagcgccag 2401 tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc cctgccagtc aagattcagc 2461 ttcagacgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag 2521 gagtgtttat catcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga 2581 tcgttgagta catgcaggcc attaccgcgc cagacaatca ccagctcaca aaaatcatgt 2641 gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg 2701 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc 2761 agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta 2821 ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt gaccagttaa actctcggcg 2881 aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag cgataggcga tgtcagtaac 2941 gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc ggttcaggta 3001 tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag 3061 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca 3121 ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt 3181 acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc 3241 attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct 3301 gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt 3361 cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag 3421 attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc 3481 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac 3541 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat 3601 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc 3661 ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt 3721 ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga 3781 aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct 3841 ttccctggtt gccaatggcc cattttcttg tcagtaacga gaaggtcgcg aatccaggcg 3901 ctttttagac tggtcgtata ctagagatta aagaggagaa acggacgatg aagtacctgc 3961 tgccgaccgc tgctgctggt ctgctgctcc tcgctgccca gccggcgatg gcccgtaaag 4021 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg 4081 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc 4141 tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg gtaacgacgc 4201 tgacttatgg tgttcagtgc tttgctcgtt atccggacca catgaagcag catgacttct 4261 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg 4321 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg 4381 agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag ctggaataca 4441 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga 4501 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc 4561 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc 4621 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca catggttctg ctggagttcg 4681 taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgacca attattgaag 4741 gccgctaacg cggccttttt ttgtttctgg tctcccaaaa atttatttgc ttattaatca 4801 tccggctcgt ataatgtgtg gagggcccaa gttcacttaa aaaggagaat agttatgaaa 4861 tcgaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc 4921 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc 4981 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt 5041 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga 5101 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggattct ctacgccgga 5161 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac 5221 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg 5281 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctccct gcacgcacca 5341 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag 5401 gagtcgcata agggagagcg ccgtccgatg cccttgagag ccttcaaccc agtcagctcc 5461 ttccggtggg cgcggggcat gaccattgtg gccgcactta tgactgtctt ctttatcatg 5521 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc 5581 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct 5641 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc 5701 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg 5761 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc 5821 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct 5881 cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg 5941 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc 6001 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgagacaa ttgtccagat 6061 aaaaaaaatc cttagctttc gctaaggatg atttctaaaa tttttctgtc atctcttcgt 6121 tattaatgtt tgtaattgac tgaatatcaa cgcttattta aatcagactg aagactttat 6181 ctctctgtca taaaactgtc atattcctta catataactg tcacctgttt gtcctatttt 6241 gcttctcgta gccaacaaac aatgctttat gaatcctccc aggagacatt atgaaagtta 6301 tgcgtaccac cgtcgcaact gttgtcgccg cgaccttatc gatgagtgct ttctctgtgt 6361 ttgcagaagc aagcctgaca ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat 6421 gggctgacac ttaccagaaa gaaaccggta ataaagttaa ctaccagggt atcggttctt 6481 ccggtggcgt aaaacagatt atcgctaata ccgttgattt tggtgcctct gacgcgcc //