LOCUS SEGA093 6826 bp ds-DNA linear UNA 09-SEP-2021 DEFINITION natural linear DNA. ACCESSION SEGA093 VERSION SEGA093.1 KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified FEATURES Location/Qualifiers CDS 1..528 /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYP IALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEE VRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGT DVDQPRNLAKSVTVE" /label="glmS" /codon_start=1 /note="glmS" terminator 538..594 /label="L3S2P11 Terminator" /note="L3S2P11 Terminator" CDS complement(605..1753) /protein_id="NP_415278.1" /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC" /function="enzyme; Degradation of small molecules: Carbon compounds" /label="galK" /gene_synonym="ECK0746; galA; JW0740" /EC_number="2.7.1.6" /codon_start=1 /db_xref="GI:16128725" /db_xref="ASAP:ABE-0002568" /db_xref="EcoGene:EG10363" /db_xref="GeneID:945358" /db_xref="UniProtKB/Swiss-Prot:P0A6T3" /gene="galK" /note="galK" /product="galactokinase" /locus_tag="b0757" promoter complement(1783..1830) /label="EM7 promoter" /note="synthetic bacterial promoter" /note="EM7 promoter" terminator 1843..1886 /label="Terminator rrnB T1" /note="Terminator rrnB T1" CDS complement(1899..2744) /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG AIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH LNSQKD" /label="rhaR" /codon_start=1 /gene="E. coli rhaR" /note="rhaR" /note="needed for rhamnose-dependent activation of the rhaB promoter" /product="transcriptional activator of rhaR and rhaS" CDS complement(2818..3654) /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF LQ" /label="rhaS" /codon_start=1 /gene="E. coli rhaS" /note="rhaS" /note="activated by L-rhamnose" /product="positive regulator of the rhaB promoter" promoter 3800..3918 /label="rhaB promoter" /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /note="rhaB promoter" RBS 3930..3956 /note="TIR" /note="low TIR" CDS 3948..4010 /protein_id="NP_416361.2" /translation="MKKRGAFLGLLLVSACASVFA" /label="YebFss" /gene_synonym="ECK1848; JW1836" /codon_start=1 /db_xref="UniProtKB/Swiss-Prot:P33219" /db_xref="GI:145698278" /db_xref="GeneID:946363" /db_xref="ASAP:ABE-0006141" /db_xref="EcoGene:EG11807" /gene="yebF" /note="YebFss" /product="yebF signal sequence" /locus_tag="b1847" CDS 4011..4301 /translation="ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKI VGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR" /label="YebF" /codon_start=1 /note="YebF" /product="full size yebF protein" CDS 4302..5012 /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGI KANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMV LLEFVTAAGITHGMDELYK" /label="sfGFP" /codon_start=1 /note="sfGFP" /product="superfolder GFP" terminator 5016..5064 /label="L3S3P22 Terminator (synthetic)" /note="L3S3P22 Terminator" promoter 5075..5103 /label="Promoter 3BCD2" /note="Promoter 3BCD2" CDS 5143..6333 /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" /label="TcR" /codon_start=1 /gene="tet" /note="confers resistance to tetracycline" /note="TcR" /product="tetracycline efflux protein" terminator 6344..6384 /label="rrnC" /note="rrnC" CDS 6579..6826 /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" /label="pstS" /codon_start=1 /note="pstS" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccaccgta 601 ttcatcagca ctgtcctgct ccttgtgatg gtttacaaac gtaaaaagtc tctttaatac 661 ctgtttttgc ttcatattgt tcagcgacag cttgctgtac ggcaggcacc agctcttccg 721 ggatcagcgc gacgatacag ccgccaaatc cgccgccggt catgcgtacg ccacctttgt 781 cgccaatcac agctttgacg atttctacca gagtgtcaat ttgcggcacg gtgatttcga 841 aatcatcgcg catagaggca tgagactccg ccatcaactc gcccatacgt ttcaggtcgc 901 cttgctccag cgcgctggca gcttcaacgg tgcgggcgtt ttcagtcagt atatgacgca 961 cgcgttttgc cacgatcggg tccagttcat gcgcaacagc gttgaactct tcaatggtga 1021 catcacgcag ggctggctgc tggaagaaac gcgcaccggt ttcgcactgt tcacgacggg 1081 tgttgtattc gctgccaacc agggtacgtt tgaagttact gttgatgatg acgacagcca 1141 cacctttggg catggaaact gctttggtcc ccagtgagcg gcaatcgatc agcaaggcat 1201 gatctttctt gccgagcgcg gaaattagct gatccatgat cccgcagtta cagcctacaa 1261 actggttttc tgcttcctga ccgttaagcg cgatttgtgc gccgtccagc ggcagatgat 1321 aaagctgctg caatacggtt ccgaccgcga cttccagtga agcggaagaa cttaacccgg 1381 caccctgcgg cacattgccg ctgatcacca tgtccacgcc gccgaagctg ttgttacgca 1441 gttgcagatg tttcaccacg ccacgaacgt agttagccca ttgatagttt tcatgtgcga 1501 caatgggcgc atcgagggaa aactcgtcga gctgattttc ataatcggct gccatcacgc 1561 gaactttacg gtcatcgcgt ggtgcacaac tgatcacggt ttgataatca atcgcgcagg 1621 gcagaacgaa accgtcgttg tagtcggtgt gttcaccaat caaattcacg cggccaggcg 1681 cctgaatggt gtgagtggca gggtagccaa atgcgttggc aaacagagat tgtgtttttt 1741 ctttcagact catgattgcc tcctgggtta gttcctcacc ttgtcgtatt atactatgcc 1801 gatatactat gccgatgatt aattgtcaac ctttaatgaa ttataaaacg aaaggctcag 1861 tcgaaagact gggcctttcg ttttatgcgt tgcgcttaat ctttctgcga attgagatga 1921 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta 1981 tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga 2041 tattgcgcat gacacactct gacctgtcgc agatattgat tgatggtcat tccagtctgc 2101 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc 2161 agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc cagcaacgtt 2221 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc 2281 aactgcccga acagcaactc agccatttcg ttagcaaacg gcacatgctg actactttca 2341 tgctcaagct gaccaataac ctgccgcgcc tgcgccatcc ccatgctacc taagcgccag 2401 tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc cctgccagtc aagattcagc 2461 ttcagacgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag 2521 gagtgtttat catcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga 2581 tcgttgagta catgcaggcc attaccgcgc cagacaatca ccagctcaca aaaatcatgt 2641 gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg 2701 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc 2761 agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta 2821 ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt gaccagttaa actctcggcg 2881 aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag cgataggcga tgtcagtaac 2941 gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc ggttcaggta 3001 tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag 3061 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca 3121 ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt 3181 acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc 3241 attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct 3301 gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt 3361 cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag 3421 attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc 3481 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac 3541 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat 3601 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc 3661 ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt 3721 ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga 3781 aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct 3841 ttccctggtt gccaatggcc cattttcttg tcagtaacga gaaggtcgcg aatccaggcg 3901 ctttttagac tggtcgtata ctagagatta aagaggagaa accctcgatg aagaaaagag 3961 gggcgttttt agggctgttg ttggtttctg cctgcgcatc agttttcgct gcgaataacg 4021 aaacgagcaa gagcgtcacc ttcccgaagt gcgaggatct ggacgcagcg ggcattgctg 4081 ccagcgtgaa acgtgactac caacagaacc gcgtggctcg ttgggcagac gaccagaaga 4141 tcgtcggtca agcggacccg gttgcctggg tttccctgca agatatccag ggcaaagatg 4201 ataaatggag cgtcccgctg accgttcgtg gtaagagcgc ggacattcac tatcaggtga 4261 gcgtcgattg caaagccggc atggcggagt accaacgccg ccgtaaaggc gaagagctgt 4321 tcactggtgt cgtccctatt ctggtggaac tggatggtga tgtcaacggt cataagtttt 4381 ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg aagttcatct 4441 gtactactgg taaactgccg gtaccttggc cgactctggt aacgacgctg acttatggtg 4501 ttcagtgctt tgctcgttat ccggaccaca tgaagcagca tgacttcttc aagtccgcca 4561 tgccggaagg ctatgtgcag gaacgcacga tttcctttaa ggatgacggc acgtacaaaa 4621 cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca 4681 ttgactttaa agaagacggc aatatcctgg gccataagct ggaatacaat tttaacagcc 4741 acaatgttta catcaccgcc gataaacaaa aaaatggcat taaagcgaat tttaaaattc 4801 gccacaacgt ggaggatggc agcgtgcagc tggctgatca ctaccagcaa aacactccaa 4861 tcggtgatgg tcctgttctg ctgccagaca atcactatct gagcacgcaa agcgttctgt 4921 ctaaagatcc gaacgagaaa cgcgatcaca tggttctgct ggagttcgta accgcagcgg 4981 gcatcacgca tggtatggat gaactgtaca aatgaccaat tattgaaggc cgctaacgcg 5041 gccttttttt gtttctggtc tcccaaaaat ttatttgctt attaatcatc cggctcgtat 5101 aatgtgtgga gggcccaagt tcacttaaaa aggagaatag ttatgaaatc gaacaatgcg 5161 ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg 5221 gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc 5281 gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg 5341 tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac 5401 tacgcgatca tggcgaccac acccgtcctg tggattctct acgccggacg catcgtggcc 5461 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg 5521 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca 5581 ggccccgtgg ccgggggact gttgggcgcc atctccctgc acgcaccatt ccttgcggcg 5641 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag 5701 ggagagcgcc gtccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg 5761 cggggcatga ccattgtggc cgcacttatg actgtcttct ttatcatgca actcgtagga 5821 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg 5881 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc 5941 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc 6001 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt 6061 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg 6121 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta 6181 acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg 6241 aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt 6301 cgcggtgcat ggagccgggc cacctcgacc tgagacaatt gtccagataa aaaaaatcct 6361 tagctttcgc taaggatgat ttctaaaatt tttctgtcat ctcttcgtta ttaatgtttg 6421 taattgactg aatatcaacg cttatttaaa tcagactgaa gactttatct ctctgtcata 6481 aaactgtcat attccttaca tataactgtc acctgtttgt cctattttgc ttctcgtagc 6541 caacaaacaa tgctttatga atcctcccag gagacattat gaaagttatg cgtaccaccg 6601 tcgcaactgt tgtcgccgcg accttatcga tgagtgcttt ctctgtgttt gcagaagcaa 6661 gcctgacagg tgcaggtgca accttccctg cgccggtgta tgccaaatgg gctgacactt 6721 accagaaaga aaccggtaat aaagttaact accagggtat cggttcttcc ggtggcgtaa 6781 aacagattat cgctaatacc gttgattttg gtgcctctga cgcgcc //