LOCUS       SubtilinReceive         2548 bp    DNA     linear       22-Dec-2024
ACCESSION   SubtilinReceiver_spaRK_separated
FEATURES             Location/Qualifiers
     misc_feature    6..11
                     /label=EcoRI
     misc_feature    2538..2543
                     /label=NheI
     polyA_site      1..5
                     /label=PolyA_Tail
     promoter        22..132
                     /label=PspaRK
     promoter        2461..2518
                     /label=PspaS
     RBS             886..896
                     /label=RBS_SpaK
     RBS             133..142
                     /label=RBS_SpaR
     RBS             2519..2526
                     /label=RBS_SpaS
     misc_feature    12..21
                     /label=Shim_PspaRK
     misc_feature    897..906
     misc_feature    2527..2534
                     /label=Shim_RBS
     misc_feature    143..149
                     /label=Shim_SpaR
     misc_feature    816..885
                     /label=Shim_SpaR
     CDS             907..2289
                     /label=spaK
     CDS             150..815
                     /label=spaR
     polyA_site      2544..2548
                     /label=PolyA_Tail
     misc_feature    2535..2537
                     /label=met_codon
     terminator      2290..2369
                     /label=rrnB
     misc_feature    2456..2460
                     /label=rrnO_shim
     terminator      2370..2455
                     /label=rrnO
ORIGIN
        1 aaaaagaatt cctagagatc cgcatgaaat aaattcaggg gtattgatga tggtttttgg
       61 tataatatgt acaatcattg ccagtcttat atggtttagt aaatgggaag gcagaaaaac
      121 gtttgaatag tatatgaggg ggccgggaaa tggcaaaaat ccttgctgtc gatgatgaaa
      181 aagatattct tgtactgata caaaatattt tgcgccgtga ccagcatcaa gttgacattt
      241 tagatcatgt tcatggacag ccgcctgatg tatttcaagg atatgattta attttgttag
      301 atgtgatgat gccggatatt gacggttttg agttatgtaa acagatacgg cctttggttg
      361 attgtcccat tttattcctc acagctaaaa cggaagagga agcgatagta aaaggcctca
      421 ttaccggcgg agatgattat attacaaagc cgttcggcgt tagagagctt agtgccagag
      481 tgaatgctca cttaagaaga gaacggagag ataaacatca atcgaagaga gtgatctccg
      541 gttttttatt tcattttgat agtaaagaag tatttatcaa taacaacaaa ctcaacctaa
      601 caaaaaatga atataaaatc tgtgaatttt tagctcaaca caaagggaga actttttcga
      661 gagaacaaat ttacgaggag atatatggat tagaggggaa tgctttgtat tcaacaatta
      721 ctgaatttat tcgaacgatt cgaaaaaaat gtaaagagca taatgcagat cccattaaaa
      781 cggtatgggg cgttgggtac aaatgggaat aataaaggtt ctgttaagta actgaaccca
      841 atgtcgttag tgacgcttac ctcttaagag gtcactgacc taacaggtat ggggcgttgg
      901 gtacaaatgg gaataggttt taagggccgg aaaacgcttc ttagagaact ggtaaagtat
      961 atggtgaccc tatgtattag cttggtcgta cttgctctgc tatatatatt tataaatacg
     1021 attgcaatga atacaggttt ctcacaccca gcgaattata atgaacgaga agcggaaaaa
     1081 cttgcgccga agctggaaac catcgacaaa gtaactgccg atatgattcc tgatacaatg
     1141 tcatatgcca ttcttaacaa agaaacaaaa caaaaaacag ctggaactat taaagaaaaa
     1201 gatctgcagc tggtaaaaaa gaagattgaa aaaaaaccat atgtcaatta taagcaaaaa
     1261 ggctatttgg ttatagaaag aaacaatgaa tattgtgttt tgcaatattc attgcgtgcc
     1321 gatttcagtt cgcctttact aagaaaatac cttcctaatt atgaattaac gagtatttgt
     1381 attttgatta tcttgctcat tatagttatc tctattataa caacttactt tgcgaataga
     1441 ttaagaaaac attttgagac attgaacgta atcactcgat atataaaaga gcaaaatttg
     1501 caattcactc ccgaatttac acatattaag gagtttgatg atgtgattga ctcgttgatt
     1561 gaaatgagag atgctctcca gtcctcactt gaggcacagt ggcgtttaga aaaaaacaag
     1621 aaagaacaaa taggtgcttt ggcacatgat atcaagattc cgatcaccat tattaagggg
     1681 aatgctgaat tactcagctt gtcaatgcaa aacgaagaac aagctgagta tacaaagtat
     1741 attcttggag caggaaatca aattgaacag tatatttatc agctgattca tctttcgaaa
     1801 acggaggatg ccttaacgat tcatttggag aaagcatctg tagatgagtt gacagaaact
     1861 ctagtgaaag atatatccgc ctataaagga aataaaaata taaacatttc gttcaaaaaa
     1921 gaaaatctaa tgaaagaagc gaaaatagac tggcaattgt tacatagggc attattgaat
     1981 attttaacaa atgcagttga ttatacaccg gaaggcggca ccgtctcggt acatgcagaa
     2041 tgcgattctg agatatttta tttttttgta aaagacacgg gaaatggatt ttcggaaatg
     2101 gggttaaaaa aagccacaga acttttttat atggatgaca agagccgtca ttctaaaggt
     2161 cattatggaa tgggattgac ttttgcaaaa aatgcagtga atcttcataa tggagaactt
     2221 actcttggga acactatagc cggtggagca gaggttcgag taaaaatacc tttaagaaac
     2281 gaataataac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2341 ttatctgttg tttgtcggtg aacgctctca ggacgccgcc aagcaagctt aaacccagct
     2401 caatgagctg ggttttttgt ttgttaaaaa tgaagaagaa actgtgaagc gtattgatct
     2461 taaaaaaagg aaaaaattga taaaatcttg atatttgtct gttactattt aggtattgaa
     2521 aggaggtgac caatatggct agcaaaaa
//