PspaRK PspaRK PolyA_Tail_5bp PolyA_Tail RBS_SpaS RBS_SpaS PspaS_core PspaS RBS_SpaK RBS_SpaK Shim_RBS_ds Shim_RBS met_codon met_codon Shim_PspaRK Shim_PspaRK EcoRI_rs EcoRI SpaK spaK NheI_rs NheI Shim_SpaR_ds Shim_SpaR SubtilinReceiver_spaRK_separated RBS_SpaS rrnO_ter Shim_SpaR Shim_PspaRK Shim_RBS_ds rrnO_shim met_codon PolyA_Tail_5bp NheI_rs RBS_SpaK RBS_SpaR Shim_RBS_SpaK_downstream SpaR SpaK component0 rrnB_T1_ter PspaRK EcoRI_rs Shim_SpaR_ds PspaS_core SpaR_annotation range 150 815 rrnO_shim_annotation range 2456 2460 Shim_SpaR_annotation range 143 149 PolyA_Tail_5bp_annotation range 1 5 NheI_rs_annotation range 2538 2543 RBS_SpaS_annotation range 2519 2526 rrnO_ter_annotation range 2370 2455 annotation20 range 2544 2548 SpaK_annotation range 907 2289 Shim_PspaRK_annotation range 12 21 RBS_SpaR_annotation range 133 142 Shim_RBS_SpaK_downstream_annotation range 897 906 RBS_SpaK_annotation range 886 896 PspaS_core_annotation range 2461 2518 met_codon_annotation range 2535 2537 PspaRK_annotation range 22 132 rrnB_T1_ter_annotation range 2290 2369 EcoRI_rs_annotation range 6 11 Shim_RBS_ds_annotation range 2527 2534 Shim_SpaR_ds_annotation range 816 885 Shim_SpaR Shim_SpaR rrnO_shim rrnO_shim rrnB_T1_ter rrnB SpaR spaR RBS_SpaR RBS_SpaR rrnO_ter rrnO Shim_RBS_SpaK_downstream Spacing sequence between the RBS and a CDS Shim_SpaR_seq ccgggaa RBS_SpaK_seq ggtatggggcg PolyA_Tail_5bp_seq_5bp aaaaa SpaR_seq atggcaaaaatccttgctgtcgatgatgaaaaagatattcttgtactgatacaaaatattttgcgccgtgaccagcatcaagttgacattttagatcatgttcatggacagccgcctgatgtatttcaaggatatgatttaattttgttagatgtgatgatgccggatattgacggttttgagttatgtaaacagatacggcctttggttgattgtcccattttattcctcacagctaaaacggaagaggaagcgatagtaaaaggcctcattaccggcggagatgattatattacaaagccgttcggcgttagagagcttagtgccagagtgaatgctcacttaagaagagaacggagagataaacatcaatcgaagagagtgatctccggttttttatttcattttgatagtaaagaagtatttatcaataacaacaaactcaacctaacaaaaaatgaatataaaatctgtgaatttttagctcaacacaaagggagaactttttcgagagaacaaatttacgaggagatatatggattagaggggaatgctttgtattcaacaattactgaatttattcgaacgattcgaaaaaaatgtaaagagcataatgcagatcccattaaaacggtatggggcgttgggtacaaatgggaataataa PspaS_core_seq taaaaaaaggaaaaaattgataaaatcttgatatttgtctgttactatttaggtattg Shim_SpaR_ds_seq aggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaaca RBS_SpaR_seq tatgaggggg NheI_rs_seq gctagc SubtilinReceiver_spaRK_separated_seq aaaaagaattcctagagatccgcatgaaataaattcaggggtattgatgatggtttttggtataatatgtacaatcattgccagtcttatatggtttagtaaatgggaaggcagaaaaacgtttgaatagtatatgagggggccgggaaatggcaaaaatccttgctgtcgatgatgaaaaagatattcttgtactgatacaaaatattttgcgccgtgaccagcatcaagttgacattttagatcatgttcatggacagccgcctgatgtatttcaaggatatgatttaattttgttagatgtgatgatgccggatattgacggttttgagttatgtaaacagatacggcctttggttgattgtcccattttattcctcacagctaaaacggaagaggaagcgatagtaaaaggcctcattaccggcggagatgattatattacaaagccgttcggcgttagagagcttagtgccagagtgaatgctcacttaagaagagaacggagagataaacatcaatcgaagagagtgatctccggttttttatttcattttgatagtaaagaagtatttatcaataacaacaaactcaacctaacaaaaaatgaatataaaatctgtgaatttttagctcaacacaaagggagaactttttcgagagaacaaatttacgaggagatatatggattagaggggaatgctttgtattcaacaattactgaatttattcgaacgattcgaaaaaaatgtaaagagcataatgcagatcccattaaaacggtatggggcgttgggtacaaatgggaataataaaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacaggtatggggcgttgggtacaaatgggaataggttttaagggccggaaaacgcttcttagagaactggtaaagtatatggtgaccctatgtattagcttggtcgtacttgctctgctatatatatttataaatacgattgcaatgaatacaggtttctcacacccagcgaattataatgaacgagaagcggaaaaacttgcgccgaagctggaaaccatcgacaaagtaactgccgatatgattcctgatacaatgtcatatgccattcttaacaaagaaacaaaacaaaaaacagctggaactattaaagaaaaagatctgcagctggtaaaaaagaagattgaaaaaaaaccatatgtcaattataagcaaaaaggctatttggttatagaaagaaacaatgaatattgtgttttgcaatattcattgcgtgccgatttcagttcgcctttactaagaaaataccttcctaattatgaattaacgagtatttgtattttgattatcttgctcattatagttatctctattataacaacttactttgcgaatagattaagaaaacattttgagacattgaacgtaatcactcgatatataaaagagcaaaatttgcaattcactcccgaatttacacatattaaggagtttgatgatgtgattgactcgttgattgaaatgagagatgctctccagtcctcacttgaggcacagtggcgtttagaaaaaaacaagaaagaacaaataggtgctttggcacatgatatcaagattccgatcaccattattaaggggaatgctgaattactcagcttgtcaatgcaaaacgaagaacaagctgagtatacaaagtatattcttggagcaggaaatcaaattgaacagtatatttatcagctgattcatctttcgaaaacggaggatgccttaacgattcatttggagaaagcatctgtagatgagttgacagaaactctagtgaaagatatatccgcctataaaggaaataaaaatataaacatttcgttcaaaaaagaaaatctaatgaaagaagcgaaaatagactggcaattgttacatagggcattattgaatattttaacaaatgcagttgattatacaccggaaggcggcaccgtctcggtacatgcagaatgcgattctgagatattttatttttttgtaaaagacacgggaaatggattttcggaaatggggttaaaaaaagccacagaacttttttatatggatgacaagagccgtcattctaaaggtcattatggaatgggattgacttttgcaaaaaatgcagtgaatcttcataatggagaacttactcttgggaacactatagccggtggagcagaggttcgagtaaaaatacctttaagaaacgaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcaggacgccgccaagcaagcttaaacccagctcaatgagctgggttttttgtttgttaaaaatgaagaagaaactgtgaagcgtattgatcttaaaaaaaggaaaaaattgataaaatcttgatatttgtctgttactatttaggtattgaaaggaggtgaccaatatggctagcaaaaa Shim_RBS_SpaK_downstream_seq ttgggtacaa PspaRK_seq gcatgaaataaattcaggggtattgatgatggtttttggtataatatgtacaatcattgccagtcttatatggtttagtaaatgggaaggcagaaaaacgtttgaatagta RBS_SpaS_seq aaaggagg Shim_RBS_ds_seq tgaccaat SpaK_seq atgggaataggttttaagggccggaaaacgcttcttagagaactggtaaagtatatggtgaccctatgtattagcttggtcgtacttgctctgctatatatatttataaatacgattgcaatgaatacaggtttctcacacccagcgaattataatgaacgagaagcggaaaaacttgcgccgaagctggaaaccatcgacaaagtaactgccgatatgattcctgatacaatgtcatatgccattcttaacaaagaaacaaaacaaaaaacagctggaactattaaagaaaaagatctgcagctggtaaaaaagaagattgaaaaaaaaccatatgtcaattataagcaaaaaggctatttggttatagaaagaaacaatgaatattgtgttttgcaatattcattgcgtgccgatttcagttcgcctttactaagaaaataccttcctaattatgaattaacgagtatttgtattttgattatcttgctcattatagttatctctattataacaacttactttgcgaatagattaagaaaacattttgagacattgaacgtaatcactcgatatataaaagagcaaaatttgcaattcactcccgaatttacacatattaaggagtttgatgatgtgattgactcgttgattgaaatgagagatgctctccagtcctcacttgaggcacagtggcgtttagaaaaaaacaagaaagaacaaataggtgctttggcacatgatatcaagattccgatcaccattattaaggggaatgctgaattactcagcttgtcaatgcaaaacgaagaacaagctgagtatacaaagtatattcttggagcaggaaatcaaattgaacagtatatttatcagctgattcatctttcgaaaacggaggatgccttaacgattcatttggagaaagcatctgtagatgagttgacagaaactctagtgaaagatatatccgcctataaaggaaataaaaatataaacatttcgttcaaaaaagaaaatctaatgaaagaagcgaaaatagactggcaattgttacatagggcattattgaatattttaacaaatgcagttgattatacaccggaaggcggcaccgtctcggtacatgcagaatgcgattctgagatattttatttttttgtaaaagacacgggaaatggattttcggaaatggggttaaaaaaagccacagaacttttttatatggatgacaagagccgtcattctaaaggtcattatggaatgggattgacttttgcaaaaaatgcagtgaatcttcataatggagaacttactcttgggaacactatagccggtggagcagaggttcgagtaaaaatacctttaagaaacgaataataa EcoRI_rs_seq gaattc rrnO_shim_seq gatct rrnB_T1_ter_seq ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc met_codon_seq atg rrnO_ter_seq aggacgccgccaagcaagcttaaacccagctcaatgagctgggttttttgtttgttaaaaatgaagaagaaactgtgaagcgtatt Shim_PspaRK_seq ctagagatcc