iGEM 2018 Distribution Plate 2
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member: BBa_K759012
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member: iGEM 2018 Distribution Plate 2 Well 5G
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iGEM 2018 Distribution Plate 2 Well 13D
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member: BBa_J202010

iGEM 2018 Distribution Plate 2 Well 20N
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member: BBa_I732902

iGEM 2018 Distribution Plate 2 Well 4B
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member: cI lam

iGEM 2018 Distribution Plate 2 Well 17F
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member: BBa_K847101

iGEM 2018 Distribution Plate 2 Well 7O
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member: BBa_K808014

iGEM 2018 Distribution Plate 2 Well 9A
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member: BBa_K808033

iGEM 2018 Distribution Plate 2 Well 24H
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member: BBa_S01002

iGEM 2018 Distribution Plate 2 Well 8E
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member: BBa_K747026

iGEM 2018 Distribution Plate 2 Well 17J
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member: BBa_K801071

iGEM 2018 Distribution Plate 2 Well 22H
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member: BBa_I6106

iGEM 2018 Distribution Plate 2 Well 20A
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member: BBa_K747072

iGEM 2018 Distribution Plate 2 Well 9I
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member: BBa_K850002

iGEM 2018 Distribution Plate 2 Well 19K
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member: fhlA-E363

iGEM 2018 Distribution Plate 2 Well 17I
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member: BBa_K895002

iGEM 2018 Distribution Plate 2 Well 16D
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member: BBa_I14044

iGEM 2018 Distribution Plate 2 Well 7K
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member: Operon2

iGEM 2018 Distribution Plate 2 Well 4N
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member: ecfp

iGEM 2018 Distribution Plate 2 Well 21C
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member: HMG-CoA-R

iGEM 2018 Distribution Plate 2 Well 21P
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member: BBa_K737051

iGEM 2018 Distribution Plate 2 Well 12H
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member: BBa_I6060

iGEM 2018 Distribution Plate 2 Well 24C
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version: 1
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member: BBa_K747089

iGEM 2018 Distribution Plate 2 Well 6D
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member: lacI+pL

iGEM 2018 Distribution Plate 2 Well 22J
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member: BBa_I6107

iGEM 2018 Distribution Plate 2 Well 20B
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member: BBa_I13528

iGEM 2018 Distribution Plate 2 Well 19E
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member: BBa_K863204

iGEM 2018 Distribution Plate 2 Well 19I
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member: fhlA

iGEM 2018 Distribution Plate 2 Well 12O
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member: BBa_K747047

iGEM 2018 Distribution Plate 2 Well 1J
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member: BBa_K936013

iGEM 2018 Distribution Plate 2 Well 17L
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member: BBa_K801072

iGEM 2018 Distribution Plate 2 Well 10J
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member: BBa_I6032

iGEM 2018 Distribution Plate 2 Well 8C
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member: BBa_K747025

iGEM 2018 Distribution Plate 2 Well 10F
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member: BBa_I6030

iGEM 2018 Distribution Plate 2 Well 3H
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member: BBa_K801010

iGEM 2018 Distribution Plate 2 Well 15P
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member: BBa_K909003

iGEM 2018 Distribution Plate 2 Well 17H
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member: BBa_K801070

iGEM 2018 Distribution Plate 2 Well 21H
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member: BBa_K876073

iGEM 2018 Distribution Plate 2 Well 20C
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member: BBa_K747073

iGEM 2018 Distribution Plate 2 Well 5N
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member: BBa_K817000

iGEM 2018 Distribution Plate 2 Well 4E
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member: BBa_K747010

iGEM 2018 Distribution Plate 2 Well 20J
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member: BBa_J01142

iGEM 2018 Distribution Plate 2 Well 24A
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member: BBa_K747088

iGEM 2018 Distribution Plate 2 Well 16N
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member: BBa_J61051

iGEM 2018 Distribution Plate 2 Well 21D
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member: BBa_K876067

iGEM 2018 Distribution Plate 2 Well 18F
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member: BBa_J06602

iGEM 2018 Distribution Plate 2 Well 9C
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member: BBa_K808034

iGEM 2018 Distribution Plate 2 Well 14A
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member: BBa_K747048

iGEM 2018 Distribution Plate 2 Well 12M
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member: BBa_K747046

iGEM 2018 Distribution Plate 2 Well 16K
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iGEM 2018 Distribution Plate 2 Well 3M
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iGEM 2018 Distribution Plate 2 Well 23K
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iGEM 2018 Distribution Plate 2 Well 10A
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member: BBa_K747032

iGEM 2018 Distribution Plate 2 Well 15J
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member: BBa_K936020

iGEM 2018 Distribution Plate 2 Well 1C
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member: BBa_K598014

iGEM 2018 Distribution Plate 2 Well 3C
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member: BBa_K519030

iGEM 2018 Distribution Plate 2 Well 10B
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member: BBa_P0153

iGEM 2018 Distribution Plate 2 Well 22O
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member: BBa_K747087

iGEM 2018 Distribution Plate 2 Well 2D
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member: BBa_B0013

iGEM 2018 Distribution Plate 2 Well 1H
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member: BBa_K812032

iGEM 2018 Distribution Plate 2 Well 4F
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member: c2 P22

iGEM 2018 Distribution Plate 2 Well 19P
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member: BBa_K876060

iGEM 2018 Distribution Plate 2 Well 9J
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member: BBa_K916000

iGEM 2018 Distribution Plate 2 Well 12K
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version: 1
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member: BBa_K747045

iGEM 2018 Distribution Plate 2 Well 13H
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display id: iGEM_2018_Plate2_Well13H
version: 1
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member: BBa_J202012

iGEM 2018 Distribution Plate 2 Well 5L
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display id: iGEM_2018_Plate2_Well5L
version: 1
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member: BBa_K876070

iGEM 2018 Distribution Plate 2 Well 22B
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display id: iGEM_2018_Plate2_Well22B
version: 1
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member: BBa_I732903

iGEM 2018 Distribution Plate 2 Well 5F
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persistent identity: iGEM 2018 Plate2 Well5F
display id: iGEM_2018_Plate2_Well5F
version: 1
owned by: myers
member: BBa_K876015

iGEM 2018 Distribution Plate 2 Well 13I
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persistent identity: iGEM 2018 Plate2 Well13I
display id: iGEM_2018_Plate2_Well13I
version: 1
owned by: myers
member: BBa_K863000

iGEM 2018 Distribution Plate 2 Well 18C
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persistent identity: iGEM 2018 Plate2 Well18C
display id: iGEM_2018_Plate2_Well18C
version: 1
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member: BBa_K747065

iGEM 2018 Distribution Plate 2 Well 12B
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persistent identity: iGEM 2018 Plate2 Well12B
display id: iGEM_2018_Plate2_Well12B
version: 1
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member: BBa_I6046

iGEM 2018 Distribution Plate 2 Well 7H
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persistent identity: iGEM 2018 Plate2 Well7H
display id: iGEM_2018_Plate2_Well7H
version: 1
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member: BBa_K779500

iGEM 2018 Distribution Plate 2 Well 20H
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display id: iGEM_2018_Plate2_Well20H
version: 1
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member: BBa_J01141

iGEM 2018 Distribution Plate 2 Well 5M
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display id: iGEM_2018_Plate2_Well5M
version: 1
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member: BBa_K731250

iGEM 2018 Distribution Plate 2 Well 1G
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persistent identity: iGEM 2018 Plate2 Well1G
display id: iGEM_2018_Plate2_Well1G
version: 1
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member: BBa_K598020

iGEM 2018 Distribution Plate 2 Well 22M
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display id: iGEM_2018_Plate2_Well22M
version: 1
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member: BBa_K747086

iGEM 2018 Distribution Plate 2 Well 8I
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persistent identity: iGEM 2018 Plate2 Well8I
display id: iGEM_2018_Plate2_Well8I
version: 1
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member: BBa_K747028

iGEM 2018 Distribution Plate 2 Well 4C
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persistent identity: iGEM 2018 Plate2 Well4C
display id: iGEM_2018_Plate2_Well4C
version: 1
owned by: myers
member: BBa_K747009

iGEM 2018 Distribution Plate 2 Well 11K
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persistent identity: iGEM 2018 Plate2 Well11K
display id: iGEM_2018_Plate2_Well11K
version: 1
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member: BBa_K750000

iGEM 2018 Distribution Plate 2 Well 2J
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persistent identity: iGEM 2018 Plate2 Well2J
display id: iGEM_2018_Plate2_Well2J
version: 1
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member: BBa_B0032

iGEM 2018 Distribution Plate 2 Well 8J
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display id: iGEM_2018_Plate2_Well8J
version: 1
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member: BBa_S0110

iGEM 2018 Distribution Plate 2 Well 22K
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persistent identity: iGEM 2018 Plate2 Well22K
display id: iGEM_2018_Plate2_Well22K
version: 1
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member: BBa_K747085

iGEM 2018 Distribution Plate 2 Well 3K
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persistent identity: iGEM 2018 Plate2 Well3K
display id: iGEM_2018_Plate2_Well3K
version: 1
owned by: myers
member: BBa_K561000

iGEM 2018 Distribution Plate 2 Well 23C
type: Collection
persistent identity: iGEM 2018 Plate2 Well23C
display id: iGEM_2018_Plate2_Well23C
version: 1
owned by: myers
member: BBa_K953002

iGEM 2018 Distribution Plate 2 Well 10E
type: Collection
persistent identity: iGEM 2018 Plate2 Well10E
display id: iGEM_2018_Plate2_Well10E
version: 1
owned by: myers
member: BBa_K747034

iGEM 2018 Distribution Plate 2 Well 17O
type: Collection
persistent identity: iGEM 2018 Plate2 Well17O
display id: iGEM_2018_Plate2_Well17O
version: 1
owned by: myers
member: BBa_K741008

iGEM 2018 Distribution Plate 2 Well 7J
type: Collection
persistent identity: iGEM 2018 Plate2 Well7J
display id: iGEM_2018_Plate2_Well7J
version: 1
owned by: myers
member: BBa_K779501

iGEM 2018 Distribution Plate 2 Well 16O
type: Collection
persistent identity: iGEM 2018 Plate2 Well16O
display id: iGEM_2018_Plate2_Well16O
version: 1
owned by: myers
member: BBa_K747063

iGEM 2018 Distribution Plate 2 Well 3F
type: Collection
persistent identity: iGEM 2018 Plate2 Well3F
display id: iGEM_2018_Plate2_Well3F
version: 1
owned by: myers
member: BBa_K880005

iGEM 2018 Distribution Plate 2 Well 21M
type: Collection
persistent identity: iGEM 2018 Plate2 Well21M
display id: iGEM_2018_Plate2_Well21M
version: 1
owned by: myers
member: BBa_K864680

iGEM 2018 Distribution Plate 2 Well 13F
type: Collection
persistent identity: iGEM 2018 Plate2 Well13F
display id: iGEM_2018_Plate2_Well13F
version: 1
owned by: myers
member: BBa_J202011

iGEM 2018 Distribution Plate 2 Well 8G
type: Collection
persistent identity: iGEM 2018 Plate2 Well8G
display id: iGEM_2018_Plate2_Well8G
version: 1
owned by: myers
member: BBa_K747027

iGEM 2018 Distribution Plate 2 Well 7E
type: Collection
persistent identity: iGEM 2018 Plate2 Well7E
display id: iGEM_2018_Plate2_Well7E
version: 1
owned by: myers
member: Ara

iGEM 2018 Distribution Plate 2 Well 19D
type: Collection
persistent identity: iGEM 2018 Plate2 Well19D
display id: iGEM_2018_Plate2_Well19D
version: 1
owned by: myers
member: BBa_K902059

iGEM 2018 Distribution Plate 2 Well 18A
type: Collection
persistent identity: iGEM 2018 Plate2 Well18A
display id: iGEM_2018_Plate2_Well18A
version: 1
owned by: myers
member: BBa_K747064

iGEM 2018 Distribution Plate 2 Well 19H
type: Collection
persistent identity: iGEM 2018 Plate2 Well19H
display id: iGEM_2018_Plate2_Well19H
version: 1
owned by: myers
member: BBa_K876011

iGEM 2018 Distribution Plate 2 Well 18P
type: Collection
persistent identity: iGEM 2018 Plate2 Well18P
display id: iGEM_2018_Plate2_Well18P
version: 1
owned by: myers
member: BBa_I7108

iGEM 2018 Distribution Plate 2 Well 16M
type: Collection
persistent identity: iGEM 2018 Plate2 Well16M
display id: iGEM_2018_Plate2_Well16M
version: 1
owned by: myers
member: BBa_K747062

iGEM 2018 Distribution Plate 2 Well 10C
type: Collection
persistent identity: iGEM 2018 Plate2 Well10C
display id: iGEM_2018_Plate2_Well10C
version: 1
owned by: myers
member: BBa_K747033

iGEM 2018 Distribution Plate 2 Well 6N
type: Collection
persistent identity: iGEM 2018 Plate2 Well6N
display id: iGEM_2018_Plate2_Well6N
version: 1
owned by: myers
member: lux pR

iGEM 2018 Distribution Plate 2 Well 17B
type: Collection
persistent identity: iGEM 2018 Plate2 Well17B
display id: iGEM_2018_Plate2_Well17B
version: 1
owned by: myers
member: BBa_K909006

iGEM 2018 Distribution Plate 2 Well 13O
type: Collection
persistent identity: iGEM 2018 Plate2 Well13O
display id: iGEM_2018_Plate2_Well13O
version: 1
owned by: myers
member: BBa_K863006

iGEM 2018 Distribution Plate 2 Well 15L
type: Collection
persistent identity: iGEM 2018 Plate2 Well15L
display id: iGEM_2018_Plate2_Well15L
version: 1
owned by: myers
member: BBa_K876002

iGEM 2018 Distribution Plate 2 Well 20E
type: Collection
persistent identity: iGEM 2018 Plate2 Well20E
display id: iGEM_2018_Plate2_Well20E
version: 1
owned by: myers
member: BBa_K747074

iGEM 2018 Distribution Plate 2 Well 2L
type: Collection
persistent identity: iGEM 2018 Plate2 Well2L
display id: iGEM_2018_Plate2_Well2L
version: 1
owned by: myers
member: BBa_B0033

iGEM 2018 Distribution Plate 2 Well 4K
type: Collection
persistent identity: iGEM 2018 Plate2 Well4K
display id: iGEM_2018_Plate2_Well4K
version: 1
owned by: myers
member: BBa_K747013

iGEM 2018 Distribution Plate 2 Well 12P
type: Collection
persistent identity: iGEM 2018 Plate2 Well12P
display id: iGEM_2018_Plate2_Well12P
version: 1
owned by: myers
member: BBa_I6076

iGEM 2018 Distribution Plate 2 Well 19J
type: Collection
persistent identity: iGEM 2018 Plate2 Well19J
display id: iGEM_2018_Plate2_Well19J
version: 1
owned by: myers
member: BBa_K876013

iGEM 2018 Distribution Plate 2 Well 4P
type: Collection
persistent identity: iGEM 2018 Plate2 Well4P
display id: iGEM_2018_Plate2_Well4P
version: 1
owned by: myers
member: ECFP

iGEM 2018 Distribution Plate 2 Well 17K
type: Collection
persistent identity: iGEM 2018 Plate2 Well17K
display id: iGEM_2018_Plate2_Well17K
version: 1
owned by: myers
member: BBa_K861110

iGEM 2018 Distribution Plate 2 Well 2N
type: Collection
persistent identity: iGEM 2018 Plate2 Well2N
display id: iGEM_2018_Plate2_Well2N
version: 1
owned by: myers
member: lacI

iGEM 2018 Distribution Plate 2 Well 18G
type: Collection
persistent identity: iGEM 2018 Plate2 Well18G
display id: iGEM_2018_Plate2_Well18G
version: 1
owned by: myers
member: BBa_K747067

iGEM 2018 Distribution Plate 2 Well 11C
type: Collection
persistent identity: iGEM 2018 Plate2 Well11C
display id: iGEM_2018_Plate2_Well11C
version: 1
owned by: myers
member: phaC1-A-B1

iGEM 2018 Distribution Plate 2 Well 24F
type: Collection
persistent identity: iGEM 2018 Plate2 Well24F
display id: iGEM_2018_Plate2_Well24F
version: 1
owned by: myers
member: BBa_S01003

iGEM 2018 Distribution Plate 2 Well 13N
type: Collection
persistent identity: iGEM 2018 Plate2 Well13N
display id: iGEM_2018_Plate2_Well13N
version: 1
owned by: myers
member: BBa_K909009

iGEM 2018 Distribution Plate 2 Well 8N
type: Collection
persistent identity: iGEM 2018 Plate2 Well8N
display id: iGEM_2018_Plate2_Well8N
version: 1
owned by: myers
member: BBa_S0117

iGEM 2018 Distribution Plate 2 Well 5H
type: Collection
persistent identity: iGEM 2018 Plate2 Well5H
display id: iGEM_2018_Plate2_Well5H
version: 1
owned by: myers
member: BBa_K876057

iGEM 2018 Distribution Plate 2 Well 9G
type: Collection
persistent identity: iGEM 2018 Plate2 Well9G
display id: iGEM_2018_Plate2_Well9G
version: 1
owned by: myers
member: BBa_K850000

iGEM 2018 Distribution Plate 2 Well 16F
type: Collection
persistent identity: iGEM 2018 Plate2 Well16F
display id: iGEM_2018_Plate2_Well16F
version: 1
owned by: myers
member: BBa_I13013

iGEM 2018 Distribution Plate 2 Well 11B
type: Collection
persistent identity: iGEM 2018 Plate2 Well11B
display id: iGEM_2018_Plate2_Well11B
version: 1
owned by: myers
member: BBa_J202000

iGEM 2018 Distribution Plate 2 Well 18N
type: Collection
persistent identity: iGEM 2018 Plate2 Well18N
display id: iGEM_2018_Plate2_Well18N
version: 1
owned by: myers
member: BBa_I13542

iGEM 2018 Distribution Plate 2 Well 9N
type: Collection
persistent identity: iGEM 2018 Plate2 Well9N
display id: iGEM_2018_Plate2_Well9N
version: 1
owned by: myers
member: BBa_K747095

iGEM 2018 Distribution Plate 2 Well 6F
type: Collection
persistent identity: iGEM 2018 Plate2 Well6F
display id: iGEM_2018_Plate2_Well6F
version: 1
owned by: myers
member: p(tetR)

iGEM 2018 Distribution Plate 2 Well 2M
type: Collection
persistent identity: iGEM 2018 Plate2 Well2M
display id: iGEM_2018_Plate2_Well2M
version: 1
owned by: myers
member: BBa_K747006

iGEM 2018 Distribution Plate 2 Well 22D
type: Collection
persistent identity: iGEM 2018 Plate2 Well22D
display id: iGEM_2018_Plate2_Well22D
version: 1
owned by: myers
member: BBa_I732018

iGEM 2018 Distribution Plate 2 Well 17A
type: Collection
persistent identity: iGEM 2018 Plate2 Well17A
display id: iGEM_2018_Plate2_Well17A
version: 1
owned by: myers
member: BBa_K759012

iGEM 2018 Distribution Plate 2 Well 13B
type: Collection
persistent identity: iGEM 2018 Plate2 Well13B
display id: iGEM_2018_Plate2_Well13B
version: 1
owned by: myers
member: BBa_J202009

iGEM 2018 Distribution Plate 2 Well 22P
type: Collection
persistent identity: iGEM 2018 Plate2 Well22P
display id: iGEM_2018_Plate2_Well22P
version: 1
owned by: myers
member: BBa_I6211

iGEM 2018 Distribution Plate 2 Well 4I
type: Collection
persistent identity: iGEM 2018 Plate2 Well4I
display id: iGEM_2018_Plate2_Well4I
version: 1
owned by: myers
member: BBa_K747012

iGEM 2018 Distribution Plate 2 Well 5P
type: Collection
persistent identity: iGEM 2018 Plate2 Well5P
display id: iGEM_2018_Plate2_Well5P
version: 1
owned by: myers
member: BBa_K844000

iGEM 2018 Distribution Plate 2 Well 3G
type: Collection
persistent identity: iGEM 2018 Plate2 Well3G
display id: iGEM_2018_Plate2_Well3G
version: 1
owned by: myers
member: BBa_K574006

iGEM 2018 Distribution Plate 2 Well 16J
type: Collection
persistent identity: iGEM 2018 Plate2 Well16J
display id: iGEM_2018_Plate2_Well16J
version: 1
owned by: myers
member: BBa_J37019

iGEM 2018 Distribution Plate 2 Well 24L
type: Collection
persistent identity: iGEM 2018 Plate2 Well24L
display id: iGEM_2018_Plate2_Well24L
version: 1
owned by: myers
member: BBa_I9026

iGEM 2018 Distribution Plate 2 Well 14P
type: Collection
persistent identity: iGEM 2018 Plate2 Well14P
display id: iGEM_2018_Plate2_Well14P
version: 1
owned by: myers
member: mRFP1

iGEM 2018 Distribution Plate 2 Well 24D
type: Collection
persistent identity: iGEM 2018 Plate2 Well24D
display id: iGEM_2018_Plate2_Well24D
version: 1
owned by: myers
member: GFP genera

iGEM 2018 Distribution Plate 2 Well 20D
type: Collection
persistent identity: iGEM 2018 Plate2 Well20D
display id: iGEM_2018_Plate2_Well20D
version: 1
owned by: myers
member: BBa_M30011

iGEM 2018 Distribution Plate 2 Well 9P
type: Collection
persistent identity: iGEM 2018 Plate2 Well9P
display id: iGEM_2018_Plate2_Well9P
version: 1
owned by: myers
member: BBa_K911003

iGEM 2018 Distribution Plate 2 Well 9D
type: Collection
persistent identity: iGEM 2018 Plate2 Well9D
display id: iGEM_2018_Plate2_Well9D
version: 1
owned by: myers
member: BBa_K737032

iGEM 2018 Distribution Plate 2 Well 13K
type: Collection
persistent identity: iGEM 2018 Plate2 Well13K
display id: iGEM_2018_Plate2_Well13K
version: 1
owned by: myers
member: BBa_K863001

iGEM 2018 Distribution Plate 2 Well 17N
type: Collection
persistent identity: iGEM 2018 Plate2 Well17N
display id: iGEM_2018_Plate2_Well17N
version: 1
owned by: myers
member: BBa_K801092

iGEM 2018 Distribution Plate 2 Well 10L
type: Collection
persistent identity: iGEM 2018 Plate2 Well10L
display id: iGEM_2018_Plate2_Well10L
version: 1
owned by: myers
member: BBa_I6040

iGEM 2018 Distribution Plate 2 Well 16G
type: Collection
persistent identity: iGEM 2018 Plate2 Well16G
display id: iGEM_2018_Plate2_Well16G
version: 1
owned by: myers
member: BBa_K747059

iGEM 2018 Distribution Plate 2 Well 2O
type: Collection
persistent identity: iGEM 2018 Plate2 Well2O
display id: iGEM_2018_Plate2_Well2O
version: 1
owned by: myers
member: BBa_K747007

iGEM 2018 Distribution Plate 2 Well 13M
type: Collection
persistent identity: iGEM 2018 Plate2 Well13M
display id: iGEM_2018_Plate2_Well13M
version: 1
owned by: myers
member: BBa_K863005

iGEM 2018 Distribution Plate 2 Well 7P
type: Collection
persistent identity: iGEM 2018 Plate2 Well7P
display id: iGEM_2018_Plate2_Well7P
version: 1
owned by: myers
member: PCaa3

iGEM 2018 Distribution Plate 2 Well 18B
type: Collection
persistent identity: iGEM 2018 Plate2 Well18B
display id: iGEM_2018_Plate2_Well18B
version: 1
owned by: myers
member: BBa_J13023

iGEM 2018 Distribution Plate 2 Well 12L
type: Collection
persistent identity: iGEM 2018 Plate2 Well12L
display id: iGEM_2018_Plate2_Well12L
version: 1
owned by: myers
member: BBa_I6066

iGEM 2018 Distribution Plate 2 Well 17M
type: Collection
persistent identity: iGEM 2018 Plate2 Well17M
display id: iGEM_2018_Plate2_Well17M
version: 1
owned by: myers
member: BBa_K741002

iGEM 2018 Distribution Plate 2 Well 18I
type: Collection
persistent identity: iGEM 2018 Plate2 Well18I
display id: iGEM_2018_Plate2_Well18I
version: 1
owned by: myers
member: BBa_K747068

iGEM 2018 Distribution Plate 2 Well 14I
type: Collection
persistent identity: iGEM 2018 Plate2 Well14I
display id: iGEM_2018_Plate2_Well14I
version: 1
owned by: myers
member: BBa_K747052

iGEM 2018 Distribution Plate 2 Well 7L
type: Collection
persistent identity: iGEM 2018 Plate2 Well7L
display id: iGEM_2018_Plate2_Well7L
version: 1
owned by: myers
member: BBa_K779503

iGEM 2018 Distribution Plate 2 Well 16P
type: Collection
persistent identity: iGEM 2018 Plate2 Well16P
display id: iGEM_2018_Plate2_Well16P
version: 1
owned by: myers
member: BBa_J04421

iGEM 2018 Distribution Plate 2 Well 4G
type: Collection
persistent identity: iGEM 2018 Plate2 Well4G
display id: iGEM_2018_Plate2_Well4G
version: 1
owned by: myers
member: BBa_K747011

iGEM 2018 Distribution Plate 2 Well 15O
type: Collection
persistent identity: iGEM 2018 Plate2 Well15O
display id: iGEM_2018_Plate2_Well15O
version: 1
owned by: myers
member: BBa_K797000

iGEM 2018 Distribution Plate 2 Well 3N
type: Collection
persistent identity: iGEM 2018 Plate2 Well3N
display id: iGEM_2018_Plate2_Well3N
version: 1
owned by: myers
member: BBa_K784013

iGEM 2018 Distribution Plate 2 Well 24O
type: Collection
persistent identity: iGEM 2018 Plate2 Well24O
display id: iGEM_2018_Plate2_Well24O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 2 Well 8H
type: Collection
persistent identity: iGEM 2018 Plate2 Well8H
display id: iGEM_2018_Plate2_Well8H
version: 1
owned by: myers
member: BBa_S0109

iGEM 2018 Distribution Plate 2 Well 24K
type: Collection
persistent identity: iGEM 2018 Plate2 Well24K
display id: iGEM_2018_Plate2_Well24K
version: 1
owned by: myers
member: BBa_K747093

iGEM 2018 Distribution Plate 2 Well 2I
type: Collection
persistent identity: iGEM 2018 Plate2 Well2I
display id: iGEM_2018_Plate2_Well2I
version: 1
owned by: myers
member: BBa_K747004

iGEM 2018 Distribution Plate 2 Well 1K
type: Collection
persistent identity: iGEM 2018 Plate2 Well1K
display id: iGEM_2018_Plate2_Well1K
version: 1
owned by: myers
member: BBa_K567008

iGEM 2018 Distribution Plate 2 Well 6C
type: Collection
persistent identity: iGEM 2018 Plate2 Well6C
display id: iGEM_2018_Plate2_Well6C
version: 1
owned by: myers
member: BBa_K747017

iGEM 2018 Distribution Plate 2 Well 23E
type: Collection
persistent identity: iGEM 2018 Plate2 Well23E
display id: iGEM_2018_Plate2_Well23E
version: 1
owned by: myers
member: RO

iGEM 2018 Distribution Plate 2 Well 1O
type: Collection
persistent identity: iGEM 2018 Plate2 Well1O
display id: iGEM_2018_Plate2_Well1O
version: 1
owned by: myers
member: BBa_K567018

iGEM 2018 Distribution Plate 2 Well 23I
type: Collection
persistent identity: iGEM 2018 Plate2 Well23I
display id: iGEM_2018_Plate2_Well23I
version: 1
owned by: myers
member: BBa_K737004

iGEM 2018 Distribution Plate 2 Well 15C
type: Collection
persistent identity: iGEM 2018 Plate2 Well15C
display id: iGEM_2018_Plate2_Well15C
version: 1
owned by: myers
member: BBa_K863011

iGEM 2018 Distribution Plate 2 Well 2H
type: Collection
persistent identity: iGEM 2018 Plate2 Well2H
display id: iGEM_2018_Plate2_Well2H
version: 1
owned by: myers
member: BBa_B0031

iGEM 2018 Distribution Plate 2 Well 16I
type: Collection
persistent identity: iGEM 2018 Plate2 Well16I
display id: iGEM_2018_Plate2_Well16I
version: 1
owned by: myers
member: BBa_K747060

iGEM 2018 Distribution Plate 2 Well 14B
type: Collection
persistent identity: iGEM 2018 Plate2 Well14B
display id: iGEM_2018_Plate2_Well14B
version: 1
owned by: myers
member: BBa_I6080

iGEM 2018 Distribution Plate 2 Well 14K
type: Collection
persistent identity: iGEM 2018 Plate2 Well14K
display id: iGEM_2018_Plate2_Well14K
version: 1
owned by: myers
member: BBa_K747053

iGEM 2018 Distribution Plate 2 Well 18E
type: Collection
persistent identity: iGEM 2018 Plate2 Well18E
display id: iGEM_2018_Plate2_Well18E
version: 1
owned by: myers
member: BBa_K747066

iGEM 2018 Distribution Plate 2 Well 17P
type: Collection
persistent identity: iGEM 2018 Plate2 Well17P
display id: iGEM_2018_Plate2_Well17P
version: 1
owned by: myers
member: BBa_K801093

iGEM 2018 Distribution Plate 2 Well 23A
type: Collection
persistent identity: iGEM 2018 Plate2 Well23A
display id: iGEM_2018_Plate2_Well23A
version: 1
owned by: myers
member: BBa_K917014

iGEM 2018 Distribution Plate 2 Well 15E
type: Collection
persistent identity: iGEM 2018 Plate2 Well15E
display id: iGEM_2018_Plate2_Well15E
version: 1
owned by: myers
member: BBa_K863020

iGEM 2018 Distribution Plate 2 Well 4D
type: Collection
persistent identity: iGEM 2018 Plate2 Well4D
display id: iGEM_2018_Plate2_Well4D
version: 1
owned by: myers
member: cI 434

iGEM 2018 Distribution Plate 2 Well 19B
type: Collection
persistent identity: iGEM 2018 Plate2 Well19B
display id: iGEM_2018_Plate2_Well19B
version: 1
owned by: myers
member: BBa_K784047

iGEM 2018 Distribution Plate 2 Well 2K
type: Collection
persistent identity: iGEM 2018 Plate2 Well2K
display id: iGEM_2018_Plate2_Well2K
version: 1
owned by: myers
member: BBa_K747005

iGEM 2018 Distribution Plate 2 Well 12D
type: Collection
persistent identity: iGEM 2018 Plate2 Well12D
display id: iGEM_2018_Plate2_Well12D
version: 1
owned by: myers
member: BBa_I6050

iGEM 2018 Distribution Plate 2 Well 6E
type: Collection
persistent identity: iGEM 2018 Plate2 Well6E
display id: iGEM_2018_Plate2_Well6E
version: 1
owned by: myers
member: BBa_K747018

iGEM 2018 Distribution Plate 2 Well 11D
type: Collection
persistent identity: iGEM 2018 Plate2 Well11D
display id: iGEM_2018_Plate2_Well11D
version: 1
owned by: myers
member: BBa_J202002

iGEM 2018 Distribution Plate 2 Well 2B
type: Collection
persistent identity: iGEM 2018 Plate2 Well2B
display id: iGEM_2018_Plate2_Well2B
version: 1
owned by: myers
member: BBa_B0012

iGEM 2018 Distribution Plate 2 Well 7M
type: Collection
persistent identity: iGEM 2018 Plate2 Well7M
display id: iGEM_2018_Plate2_Well7M
version: 1
owned by: myers
member: BBa_K808012

iGEM 2018 Distribution Plate 2 Well 8F
type: Collection
persistent identity: iGEM 2018 Plate2 Well8F
display id: iGEM_2018_Plate2_Well8F
version: 1
owned by: myers
member: BBa_S0108

iGEM 2018 Distribution Plate 2 Well 3P
type: Collection
persistent identity: iGEM 2018 Plate2 Well3P
display id: iGEM_2018_Plate2_Well3P
version: 1
owned by: myers
member: BBa_K784014

iGEM 2018 Distribution Plate 2 Well 13E
type: Collection
persistent identity: iGEM 2018 Plate2 Well13E
display id: iGEM_2018_Plate2_Well13E
version: 1
owned by: myers
member: BBa_K769020

iGEM 2018 Distribution Plate 2 Well 5J
type: Collection
persistent identity: iGEM 2018 Plate2 Well5J
display id: iGEM_2018_Plate2_Well5J
version: 1
owned by: myers
member: BBa_K876069

iGEM 2018 Distribution Plate 2 Well 21F
type: Collection
persistent identity: iGEM 2018 Plate2 Well21F
display id: iGEM_2018_Plate2_Well21F
version: 1
owned by: myers
member: BBa_K876068

iGEM 2018 Distribution Plate 2 Well 9L
type: Collection
persistent identity: iGEM 2018 Plate2 Well9L
display id: iGEM_2018_Plate2_Well9L
version: 1
owned by: myers
member: BBa_K747094

iGEM 2018 Distribution Plate 2 Well 1A
type: Collection
persistent identity: iGEM 2018 Plate2 Well1A
display id: iGEM_2018_Plate2_Well1A
version: 1
owned by: myers
member: BBa_K649303

iGEM 2018 Distribution Plate 2 Well 8L
type: Collection
persistent identity: iGEM 2018 Plate2 Well8L
display id: iGEM_2018_Plate2_Well8L
version: 1
owned by: myers
member: BBa_S0116

iGEM 2018 Distribution Plate 2 Well 8A
type: Collection
persistent identity: iGEM 2018 Plate2 Well8A
display id: iGEM_2018_Plate2_Well8A
version: 1
owned by: myers
member: BBa_K747024

iGEM 2018 Distribution Plate 2 Well 20F
type: Collection
persistent identity: iGEM 2018 Plate2 Well20F
display id: iGEM_2018_Plate2_Well20F
version: 1
owned by: myers
member: [pTET][loc

iGEM 2018 Distribution Plate 2 Well 9B
type: Collection
persistent identity: iGEM 2018 Plate2 Well9B
display id: iGEM_2018_Plate2_Well9B
version: 1
owned by: myers
member: BBa_K737031

iGEM 2018 Distribution Plate 2 Well 21L
type: Collection
persistent identity: iGEM 2018 Plate2 Well21L
display id: iGEM_2018_Plate2_Well21L
version: 1
owned by: myers
member: BBa_K737007

iGEM 2018 Distribution Plate 2 Well 15A
type: Collection
persistent identity: iGEM 2018 Plate2 Well15A
display id: iGEM_2018_Plate2_Well15A
version: 1
owned by: myers
member: BBa_K863010

iGEM 2018 Distribution Plate 2 Well 14F
type: Collection
persistent identity: iGEM 2018 Plate2 Well14F
display id: iGEM_2018_Plate2_Well14F
version: 1
owned by: myers
member: BBa_I6086

iGEM 2018 Distribution Plate 2 Well 21N
type: Collection
persistent identity: iGEM 2018 Plate2 Well21N
display id: iGEM_2018_Plate2_Well21N
version: 1
owned by: myers
member: BBa_K952003

iGEM 2018 Distribution Plate 2 Well 11F
type: Collection
persistent identity: iGEM 2018 Plate2 Well11F
display id: iGEM_2018_Plate2_Well11F
version: 1
owned by: myers
member: BBa_J202003

iGEM 2018 Distribution Plate 2 Well 17C
type: Collection
persistent identity: iGEM 2018 Plate2 Well17C
display id: iGEM_2018_Plate2_Well17C
version: 1
owned by: myers
member: BBa_K786002

iGEM 2018 Distribution Plate 2 Well 14M
type: Collection
persistent identity: iGEM 2018 Plate2 Well14M
display id: iGEM_2018_Plate2_Well14M
version: 1
owned by: myers
member: BBa_K747054

iGEM 2018 Distribution Plate 2 Well 13A
type: Collection
persistent identity: iGEM 2018 Plate2 Well13A
display id: iGEM_2018_Plate2_Well13A
version: 1
owned by: myers
member: TD0.01

iGEM 2018 Distribution Plate 2 Well 16A
type: Collection
persistent identity: iGEM 2018 Plate2 Well16A
display id: iGEM_2018_Plate2_Well16A
version: 1
owned by: myers
member: BBa_K747056

iGEM 2018 Distribution Plate 2 Well 16L
type: Collection
persistent identity: iGEM 2018 Plate2 Well16L
display id: iGEM_2018_Plate2_Well16L
version: 1
owned by: myers
member: BBa_R2109

iGEM 2018 Distribution Plate 2 Well 6P
type: Collection
persistent identity: iGEM 2018 Plate2 Well6P
display id: iGEM_2018_Plate2_Well6P
version: 1
owned by: myers
member: BBa_E0422

iGEM 2018 Distribution Plate 2 Well 8P
type: Collection
persistent identity: iGEM 2018 Plate2 Well8P
display id: iGEM_2018_Plate2_Well8P
version: 1
owned by: myers
member: BBa_P0152

iGEM 2018 Distribution Plate 2 Well 17E
type: Collection
persistent identity: iGEM 2018 Plate2 Well17E
display id: iGEM_2018_Plate2_Well17E
version: 1
owned by: myers
member: BBa_K786003

iGEM 2018 Distribution Plate 2 Well 6G
type: Collection
persistent identity: iGEM 2018 Plate2 Well6G
display id: iGEM_2018_Plate2_Well6G
version: 1
owned by: myers
member: BBa_K747019

iGEM 2018 Distribution Plate 2 Well 9E
type: Collection
persistent identity: iGEM 2018 Plate2 Well9E
display id: iGEM_2018_Plate2_Well9E
version: 1
owned by: myers
member: BBa_K823016

iGEM 2018 Distribution Plate 2 Well 15M
type: Collection
persistent identity: iGEM 2018 Plate2 Well15M
display id: iGEM_2018_Plate2_Well15M
version: 1
owned by: myers
member: BBa_K733012

iGEM 2018 Distribution Plate 2 Well 21I
type: Collection
persistent identity: iGEM 2018 Plate2 Well21I
display id: iGEM_2018_Plate2_Well21I
version: 1
owned by: myers
member: CMV AID pA

iGEM 2018 Distribution Plate 2 Well 8D
type: Collection
persistent identity: iGEM 2018 Plate2 Well8D
display id: iGEM_2018_Plate2_Well8D
version: 1
owned by: myers
member: BBa_S0107

iGEM 2018 Distribution Plate 2 Well 15G
type: Collection
persistent identity: iGEM 2018 Plate2 Well15G
display id: iGEM_2018_Plate2_Well15G
version: 1
owned by: myers
member: BBa_K861031

iGEM 2018 Distribution Plate 2 Well 6L
type: Collection
persistent identity: iGEM 2018 Plate2 Well6L
display id: iGEM_2018_Plate2_Well6L
version: 1
owned by: myers
member: cI+luxR

iGEM 2018 Distribution Plate 2 Well 19G
type: Collection
persistent identity: iGEM 2018 Plate2 Well19G
display id: iGEM_2018_Plate2_Well19G
version: 1
owned by: myers
member: BBa_K929003

iGEM 2018 Distribution Plate 2 Well 1M
type: Collection
persistent identity: iGEM 2018 Plate2 Well1M
display id: iGEM_2018_Plate2_Well1M
version: 1
owned by: myers
member: BBa_K567015

iGEM 2018 Distribution Plate 2 Well 12J
type: Collection
persistent identity: iGEM 2018 Plate2 Well12J
display id: iGEM_2018_Plate2_Well12J
version: 1
owned by: myers
member: BBa_I6064

iGEM 2018 Distribution Plate 2 Well 15B
type: Collection
persistent identity: iGEM 2018 Plate2 Well15B
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iGEM 2018 Distribution Plate 2 Well 16C
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iGEM 2018 Distribution Plate 2 Well 2F
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member: BBa_B0023

iGEM 2018 Distribution Plate 2 Well 18M
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member: BBa_K747070

iGEM 2018 Distribution Plate 2 Well 14N
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member: BBa_I6105

iGEM 2018 Distribution Plate 2 Well 12A
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iGEM 2018 Distribution Plate 2 Well 10N
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iGEM 2018 Distribution Plate 2 Well 21G
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member: BBa_K849004

iGEM 2018 Distribution Plate 2 Well 21B
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iGEM 2018 Distribution Plate 2 Well 7C
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iGEM 2018 Distribution Plate 2 Well 13P
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iGEM 2018 Distribution Plate 2 Well 5C
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iGEM 2018 Distribution Plate 2 Well 19N
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iGEM 2018 Distribution Plate 2 Well 2C
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iGEM 2018 Distribution Plate 2 Well 22C
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iGEM 2018 Distribution Plate 2 Well 8M
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iGEM 2018 Distribution Plate 2 Well 5I
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iGEM 2018 Distribution Plate 2 Well 11H
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iGEM 2018 Distribution Plate 2 Well 18D
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member: mCherry

iGEM 2018 Distribution Plate 2 Well 14O
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iGEM 2018 Distribution Plate 2 Well 3O
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iGEM 2018 Distribution Plate 2 Well 5G
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iGEM 2018 Distribution Plate 2 Well 17D
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member: BBa_K880002

iGEM 2018 Distribution Plate 2 Well 16E
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iGEM 2018 Distribution Plate 2 Well 12F
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member: BBa_I6056

iGEM 2018 Distribution Plate 2 Well 4L
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iGEM 2018 Distribution Plate 2 Well 11O
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iGEM 2018 Distribution Plate 2 Well 4O
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iGEM 2018 Distribution Plate 2 Well 10M
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iGEM 2018 Distribution Plate 2 Well 11J
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iGEM 2018 Distribution Plate 2 Well 2P
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iGEM 2018 Distribution Plate 2 Well 20O
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member: BBa_K747079

iGEM 2018 Distribution Plate 2 Well 14D
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member: BBa_I6084

iGEM 2018 Distribution Plate 2 Well 9F
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member: BBa_K737033

iGEM 2018 Distribution Plate 2 Well 18K
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iGEM 2018 Distribution Plate 2 Well 2G
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iGEM 2018 Distribution Plate 2 Well 24I
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iGEM 2018 Distribution Plate 2 Well 3D
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member: BBa_K773004

iGEM 2018 Distribution Plate 2 Well 21E
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iGEM 2018 Distribution Plate 2 Well 3E
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iGEM 2018 Distribution Plate 2 Well 7D
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iGEM 2018 Distribution Plate 2 Well 6A
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iGEM 2018 Distribution Plate 2 Well 5A
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iGEM 2018 Distribution Plate 2 Well 21K
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iGEM 2018 Distribution Plate 2 Well 21J
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iGEM 2018 Distribution Plate 2 Well 7N
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iGEM 2018 Distribution Plate 2 Well 4M
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iGEM 2018 Distribution Plate 2 Well 10O
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iGEM 2018 Distribution Plate 2 Well 1D
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iGEM 2018 Distribution Plate 2 Well 5O
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iGEM 2018 Distribution Plate 2 Well 9K
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member: BBa_K748003

iGEM 2018 Distribution Plate 2 Well 11L
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member: BBa_J202006

iGEM 2018 Distribution Plate 2 Well 13G
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member: BBa_K769022

iGEM 2018 Distribution Plate 2 Well 20P
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member: BBa_I732805

iGEM 2018 Distribution Plate 2 Well 22A
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member: BBa_K747080

iGEM 2018 Distribution Plate 2 Well 19F
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member: BBa_K876009

iGEM 2018 Distribution Plate 2 Well 14G
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member: BBa_K747051

iGEM 2018 Distribution Plate 2 Well 13C
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member: BBa_K763005

iGEM 2018 Distribution Plate 2 Well 3B
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member: BBa_K773003

iGEM 2018 Distribution Plate 2 Well 7B
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iGEM 2018 Distribution Plate 2 Well 3A
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iGEM 2018 Distribution Plate 2 Well 8O
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iGEM 2018 Distribution Plate 2 Well 20L
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member: BBa_I732095

iGEM 2018 Distribution Plate 2 Well 2E
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member: BBa_K747002

iGEM 2018 Distribution Plate 2 Well 23O
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member: BBa_J04450

iGEM 2018 Distribution Plate 2 Well 9M
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member: BBa_S05051

iGEM 2018 Distribution Plate 2 Well 1P
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member: BBa_K773002

iGEM 2018 Distribution Plate 2 Well 4J
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member: luxI

iGEM 2018 Distribution Plate 2 Well 11N
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member: BBa_J202007

iGEM 2018 Distribution Plate 2 Well 4H
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member: aiiA

iGEM 2018 Distribution Plate 2 Well 14E
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member: BBa_K747050

iGEM 2018 Distribution Plate 2 Well 24B
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member: GFP report

iGEM 2018 Distribution Plate 2 Well 14L
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member: BBa_I6103

iGEM 2018 Distribution Plate 2 Well 23P
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member: BBa_J04450

iGEM 2018 Distribution Plate 2 Well 10P
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member: BBa_I6044

iGEM 2018 Distribution Plate 2 Well 11A
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member: BBa_S05055

iGEM 2018 Distribution Plate 2 Well 24G
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member: BBa_K747091

iGEM 2018 Distribution Plate 2 Well 3J
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member: BBa_K801012

iGEM 2018 Distribution Plate 2 Well 3L
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member: BBa_K784006

iGEM 2018 Distribution Plate 2 Well 19O
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member: BBa_K775009

iGEM 2018 Distribution Plate 2 Well 9H
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iGEM 2018 Distribution Plate 2 Well 19C
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member: BBa_K822002

iGEM 2018 Distribution Plate 2 Well 19L
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member: BBa_K876038

iGEM 2018 Distribution Plate 2 Well 12G
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member: BBa_K747043

iGEM 2018 Distribution Plate 2 Well 6I
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member: BBa_K747020

iGEM 2018 Distribution Plate 2 Well 6H
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iGEM 2018 Distribution Plate 2 Well 20I
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member: BBa_K747076

iGEM 2018 Distribution Plate 2 Well 11G
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member: BBa_K733002

iGEM 2018 Distribution Plate 2 Well 16H
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member: BBa_J61016

iGEM 2018 Distribution Plate 2 Well 11I
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member: BBa_K733018

iGEM 2018 Distribution Plate 2 Well 1L
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member: BBa_K936000

iGEM 2018 Distribution Plate 2 Well 15F
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member: GFP-AID

iGEM 2018 Distribution Plate 2 Well 6B
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member: YFP

iGEM 2018 Distribution Plate 2 Well 12I
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member: BBa_K747044

iGEM 2018 Distribution Plate 2 Well 5B
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member: BBa_K876010

iGEM 2018 Distribution Plate 2 Well 11E
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member: BBa_K934023

iGEM 2018 Distribution Plate 2 Well 8K
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member: BBa_K747029

iGEM 2018 Distribution Plate 2 Well 15H
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member: BBa_K812020

iGEM 2018 Distribution Plate 2 Well 18L
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member: BBa_I13523

iGEM 2018 Distribution Plate 2 Well 9O
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member: LacIM

iGEM 2018 Distribution Plate 2 Well 18H
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member: BBa_I13457

iGEM 2018 Distribution Plate 2 Well 10G
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member: BBa_K747035

iGEM 2018 Distribution Plate 2 Well 24P
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member: BBa_J04450

iGEM 2018 Distribution Plate 2 Well 1B
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member: BBa_K909007

iGEM 2018 Distribution Plate 2 Well 20G
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member: BBa_K747075

iGEM 2018 Distribution Plate 2 Well 13J
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member: BBa_J202013

iGEM 2018 Distribution Plate 2 Well 22I
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member: BBa_K747084

iGEM 2018 Distribution Plate 2 Well 6K
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member: BBa_K747021

iGEM 2018 Distribution Plate 2 Well 15D
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member: BBa_K909005

iGEM 2018 Distribution Plate 2 Well 24E
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member: BBa_K747090

iGEM 2018 Distribution Plate 2 Well 15K
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member: Bxb1_Int

iGEM 2018 Distribution Plate 2 Well 5D
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persistent identity: iGEM 2018 Plate2 Well5D
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member: BBa_K876014

iGEM 2018 Distribution Plate 2 Well 4A
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persistent identity: iGEM 2018 Plate2 Well4A
display id: iGEM_2018_Plate2_Well4A
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member: BBa_K747008

iGEM 2018 Distribution Plate 2 Well 21A
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persistent identity: iGEM 2018 Plate2 Well21A
display id: iGEM_2018_Plate2_Well21A
version: 1
owned by: myers
member: BBa_K775010

iGEM 2018 Distribution Plate 2 Well 1N
type: Collection
persistent identity: iGEM 2018 Plate2 Well1N
display id: iGEM_2018_Plate2_Well1N
version: 1
owned by: myers
member: BBa_K876000

iGEM 2018 Distribution Plate 2 Well 10D
type: Collection
persistent identity: iGEM 2018 Plate2 Well10D
display id: iGEM_2018_Plate2_Well10D
version: 1
owned by: myers
member: BBa_P0440

iGEM 2018 Distribution Plate 2 Well 24J
type: Collection
persistent identity: iGEM 2018 Plate2 Well24J
display id: iGEM_2018_Plate2_Well24J
version: 1
owned by: myers
member: BBa_C0261

iGEM 2018 Distribution Plate 2 Well 12C
type: Collection
persistent identity: iGEM 2018 Plate2 Well12C
display id: iGEM_2018_Plate2_Well12C
version: 1
owned by: myers
member: BBa_K747041

iGEM 2018 Distribution Plate 2 Well 22F
type: Collection
persistent identity: iGEM 2018 Plate2 Well22F
display id: iGEM_2018_Plate2_Well22F
version: 1
owned by: myers
member: BBa_I732810

iGEM 2018 Distribution Plate 2 Well 7I
type: Collection
persistent identity: iGEM 2018 Plate2 Well7I
display id: iGEM_2018_Plate2_Well7I
version: 1
owned by: myers
member: tctA_505aa

iGEM 2018 Distribution Plate 2 Well 7G
type: Collection
persistent identity: iGEM 2018 Plate2 Well7G
display id: iGEM_2018_Plate2_Well7G
version: 1
owned by: myers
member: tctA_503aa

iGEM 2018 Distribution Plate 2 Well 7F
type: Collection
persistent identity: iGEM 2018 Plate2 Well7F
display id: iGEM_2018_Plate2_Well7F
version: 1
owned by: myers
member: BBa_K844008

iGEM 2018 Distribution Plate 2 Well 20M
type: Collection
persistent identity: iGEM 2018 Plate2 Well20M
display id: iGEM_2018_Plate2_Well20M
version: 1
owned by: myers
member: BBa_K747078

iGEM 2018 Distribution Plate 2 Well 21O
type: Collection
persistent identity: iGEM 2018 Plate2 Well21O
display id: iGEM_2018_Plate2_Well21O
version: 1
owned by: myers
member: BBa_K864444

iGEM 2018 Distribution Plate 2 Well 22E
type: Collection
persistent identity: iGEM 2018 Plate2 Well22E
display id: iGEM_2018_Plate2_Well22E
version: 1
owned by: myers
member: BBa_K747082

iGEM 2018 Distribution Plate 2 Well 5K
type: Collection
persistent identity: iGEM 2018 Plate2 Well5K
display id: iGEM_2018_Plate2_Well5K
version: 1
owned by: myers
member: araC-CysEm

iGEM 2018 Distribution Plate 2 Well 19A
type: Collection
persistent identity: iGEM 2018 Plate2 Well19A
display id: iGEM_2018_Plate2_Well19A
version: 1
owned by: myers
member: BBa_K741020

iGEM 2018 Distribution Plate 2 Well 6M
type: Collection
persistent identity: iGEM 2018 Plate2 Well6M
display id: iGEM_2018_Plate2_Well6M
version: 1
owned by: myers
member: BBa_K747022

iGEM 2018 Distribution Plate 2 Well 1E
type: Collection
persistent identity: iGEM 2018 Plate2 Well1E
display id: iGEM_2018_Plate2_Well1E
version: 1
owned by: myers
member: BBa_K598019

iGEM 2018 Distribution Plate 2 Well 11M
type: Collection
persistent identity: iGEM 2018 Plate2 Well11M
display id: iGEM_2018_Plate2_Well11M
version: 1
owned by: myers
member: TD1.0

iGEM 2018 Distribution Plate 2 Well 17G
type: Collection
persistent identity: iGEM 2018 Plate2 Well17G
display id: iGEM_2018_Plate2_Well17G
version: 1
owned by: myers
member: BBa_K863021

iGEM 2018 Distribution Plate 2 Well 10K
type: Collection
persistent identity: iGEM 2018 Plate2 Well10K
display id: iGEM_2018_Plate2_Well10K
version: 1
owned by: myers
member: BBa_K747037

iGEM 2018 Distribution Plate 2 Well 7A
type: Collection
persistent identity: iGEM 2018 Plate2 Well7A
display id: iGEM_2018_Plate2_Well7A
version: 1
owned by: myers
member: BBa_K731520

iGEM 2018 Distribution Plate 2 Well 2A
type: Collection
persistent identity: iGEM 2018 Plate2 Well2A
display id: iGEM_2018_Plate2_Well2A
version: 1
owned by: myers
member: BBa_K747000

iGEM 2018 Distribution Plate 2 Well 14H
type: Collection
persistent identity: iGEM 2018 Plate2 Well14H
display id: iGEM_2018_Plate2_Well14H
version: 1
owned by: myers
member: BBa_I6101

iGEM 2018 Distribution Plate 2 Well 18J
type: Collection
persistent identity: iGEM 2018 Plate2 Well18J
display id: iGEM_2018_Plate2_Well18J
version: 1
owned by: myers
member: BBa_I13541

iGEM 2018 Distribution Plate 2 Well 11P
type: Collection
persistent identity: iGEM 2018 Plate2 Well11P
display id: iGEM_2018_Plate2_Well11P
version: 1
owned by: myers
member: BBa_J202008

iGEM 2018 Distribution Plate 2 Well 12N
type: Collection
persistent identity: iGEM 2018 Plate2 Well12N
display id: iGEM_2018_Plate2_Well12N
version: 1
owned by: myers
member: BBa_I6070

iGEM 2018 Distribution Plate 2 Well 14J
type: Collection
persistent identity: iGEM 2018 Plate2 Well14J
display id: iGEM_2018_Plate2_Well14J
version: 1
owned by: myers
member: BBa_I6102

iGEM 2018 Distribution Plate 2 Well 12E
type: Collection
persistent identity: iGEM 2018 Plate2 Well12E
display id: iGEM_2018_Plate2_Well12E
version: 1
owned by: myers
member: BBa_K747042

iGEM 2018 Distribution Plate 2 Well 1I
type: Collection
persistent identity: iGEM 2018 Plate2 Well1I
display id: iGEM_2018_Plate2_Well1I
version: 1
owned by: myers
member: BBa_K567001

iGEM 2018 Distribution Plate 2 Well 16B
type: Collection
persistent identity: iGEM 2018 Plate2 Well16B
display id: iGEM_2018_Plate2_Well16B
version: 1
owned by: myers
member: BBa_I13519

iGEM 2018 Distribution Plate 2 Well 1F
type: Collection
persistent identity: iGEM 2018 Plate2 Well1F
display id: iGEM_2018_Plate2_Well1F
version: 1
owned by: myers
member: BBa_K777117

iGEM 2018 Distribution Plate 2 Well 15N
type: Collection
persistent identity: iGEM 2018 Plate2 Well15N
display id: iGEM_2018_Plate2_Well15N
version: 1
owned by: myers
member: BBa_K876004

iGEM 2018 Distribution Plate 2 Well 23G
type: Collection
persistent identity: iGEM 2018 Plate2 Well23G
display id: iGEM_2018_Plate2_Well23G
version: 1
owned by: myers
member: Cel5A

iGEM 2018 Distribution Plate 2 Well 8B
type: Collection
persistent identity: iGEM 2018 Plate2 Well8B
display id: iGEM_2018_Plate2_Well8B
version: 1
owned by: myers
member: BBa_S0100

iGEM 2018 Distribution Plate 2 Well 15I
type: Collection
persistent identity: iGEM 2018 Plate2 Well15I
display id: iGEM_2018_Plate2_Well15I
version: 1
owned by: myers
member: BBa_K861042

iGEM 2018 Distribution Plate 2 Well 6O
type: Collection
persistent identity: iGEM 2018 Plate2 Well6O
display id: iGEM_2018_Plate2_Well6O
version: 1
owned by: myers
member: BBa_K747023

iGEM 2018 Distribution Plate 2 Well 19M
type: Collection
persistent identity: iGEM 2018 Plate2 Well19M
display id: iGEM_2018_Plate2_Well19M
version: 1
owned by: myers
member: BBa_K895005

iGEM 2018 Distribution Plate 2 Well 22G
type: Collection
persistent identity: iGEM 2018 Plate2 Well22G
display id: iGEM_2018_Plate2_Well22G
version: 1
owned by: myers
member: BBa_K747083

iGEM 2018 Distribution Plate 2 Well 5E
type: Collection
persistent identity: iGEM 2018 Plate2 Well5E
display id: iGEM_2018_Plate2_Well5E
version: 1
owned by: myers
member: BBa_K775003

iGEM 2018 Distribution Plate 2 Well 22L
type: Collection
persistent identity: iGEM 2018 Plate2 Well22L
display id: iGEM_2018_Plate2_Well22L
version: 1
owned by: myers
member: BBa_I6108

iGEM 2018 Distribution Plate 2 Well 20K
type: Collection
persistent identity: iGEM 2018 Plate2 Well20K
display id: iGEM_2018_Plate2_Well20K
version: 1
owned by: myers
member: BBa_K747077

iGEM 2018 Distribution Plate 2 Well 14C
type: Collection
persistent identity: iGEM 2018 Plate2 Well14C
display id: iGEM_2018_Plate2_Well14C
version: 1
owned by: myers
member: BBa_K747049

iGEM 2018 Distribution Plate 2 Well 6J
type: Collection
persistent identity: iGEM 2018 Plate2 Well6J
display id: iGEM_2018_Plate2_Well6J
version: 1
owned by: myers
member: lux pL

iGEM 2018 Distribution Plate 2 Well 10I
type: Collection
persistent identity: iGEM 2018 Plate2 Well10I
display id: iGEM_2018_Plate2_Well10I
version: 1
owned by: myers
member: BBa_K747036

iGEM 2018 Distribution Plate 2 Well 22N
type: Collection
persistent identity: iGEM 2018 Plate2 Well22N
display id: iGEM_2018_Plate2_Well22N
version: 1
owned by: myers
member: BBa_I6207

iGEM 2018 Distribution Plate 2 Well 13L
type: Collection
persistent identity: iGEM 2018 Plate2 Well13L
display id: iGEM_2018_Plate2_Well13L
version: 1
owned by: myers
member: BBa_J202014

iGEM 2018 Distribution Plate 2 Well 18O
type: Collection
persistent identity: iGEM 2018 Plate2 Well18O
display id: iGEM_2018_Plate2_Well18O
version: 1
owned by: myers
member: BBa_K747071

iGEM 2018 Distribution Plate 2 Well 10H
type: Collection
persistent identity: iGEM 2018 Plate2 Well10H
display id: iGEM_2018_Plate2_Well10H
version: 1
owned by: myers
member: BBa_I6031

BBa_K747018
type: ComponentDefinition
persistent identity: BBa K747018
display id: BBa_K747018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 2
sequence: https://synbiohub.org/public/igem/BBa_K747018_sequence/1

AG 2
type: SequenceAnnotation
persistent identity: annotation2195969
display id: annotation2195969
version: 1
top level: BBa_K747018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747018/annotation2195969/range2195969/1

https://synbiohub.org/public/igem/BBa_K747018/annotation2195969/range2195969/1
type: Range
persistent identity: range2195969
display id: range2195969
version: 1
direction: direction/forward
top level: BBa_K747018
start: 14
end: 211
orientation: inline

BBa_P0153
type: ComponentDefinition
persistent identity: BBa P0153
display id: BBa_P0153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0167]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cII from p22. Used as the input section for Quad Part Inverters Q01530.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0053
component: BBa_B0010
component: BBa_B0012
component: BBa_B0031
sequence annotation: BBa_C0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0153_sequence/1

BBa_C0053
type: Component
persistent identity: component944580
display id: component944580
version: 1
top level: BBa_P0153
access: public
definition: c2 P22

BBa_B0010
type: Component
persistent identity: component944588
display id: component944588
version: 1
top level: BBa_P0153
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component944598
display id: component944598
version: 1
top level: BBa_P0153
access: public
definition: BBa_B0012

BBa_B0031
type: Component
persistent identity: component944568
display id: component944568
version: 1
top level: BBa_P0153
access: public
definition: BBa_B0031

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation944580
display id: annotation944580
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
type: Range
persistent identity: range944580
display id: range944580
version: 1
direction: direction/forward
top level: BBa_P0153
start: 21
end: 707
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944588
display id: annotation944588
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
type: Range
persistent identity: range944588
display id: range944588
version: 1
direction: direction/forward
top level: BBa_P0153
start: 741
end: 820
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944598
display id: annotation944598
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
type: Range
persistent identity: range944598
display id: range944598
version: 1
direction: direction/forward
top level: BBa_P0153
start: 829
end: 869
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944568
display id: annotation944568
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
type: Range
persistent identity: range944568
display id: range944568
version: 1
direction: direction/forward
top level: BBa_P0153
start: 1
end: 14
orientation: inline

BBa_K747071
type: ComponentDefinition
persistent identity: BBa K747071
display id: BBa_K747071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 5
sequence: https://synbiohub.org/public/igem/BBa_K747071_sequence/1

CT 5
type: SequenceAnnotation
persistent identity: annotation2197038
display id: annotation2197038
version: 1
top level: BBa_K747071
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747071/annotation2197038/range2197038/1

https://synbiohub.org/public/igem/BBa_K747071/annotation2197038/range2197038/1
type: Range
persistent identity: range2197038
display id: range2197038
version: 1
direction: direction/forward
top level: BBa_K747071
start: 14
end: 211
orientation: inline

BBa_S0117
type: ComponentDefinition
persistent identity: BBa S0117
display id: BBa_S0117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0031.C0053
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0053
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_S0117_sequence/1

BBa_C0053
type: Component
persistent identity: component940001
display id: component940001
version: 1
top level: BBa_S0117
access: public
definition: c2 P22

BBa_B0031
type: Component
persistent identity: component939989
display id: component939989
version: 1
top level: BBa_S0117
access: public
definition: BBa_B0031

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation939989
display id: annotation939989
version: 1
top level: BBa_S0117
location: https://synbiohub.org/public/igem/BBa_S0117/annotation939989/range939989/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_S0117/annotation939989/range939989/1
type: Range
persistent identity: range939989
display id: range939989
version: 1
direction: direction/forward
top level: BBa_S0117
start: 1
end: 14
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation940001
display id: annotation940001
version: 1
top level: BBa_S0117
location: https://synbiohub.org/public/igem/BBa_S0117/annotation940001/range940001/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_S0117/annotation940001/range940001/1
type: Range
persistent identity: range940001
display id: range940001
version: 1
direction: direction/forward
top level: BBa_S0117
start: 21
end: 707
orientation: inline

BBa_K822002
type: ComponentDefinition
persistent identity: BBa K822002
display id: BBa_K822002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Colicin E1 + immunoprotein
creator: Jarle Pahr
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1080_
m user id: 0
owner id: 11627
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Colicin E1 operon of E. coli expressed under a constitutive promoter (BBa_J23119).
mutable notes: BBa_K150007 was not available from the registry, so its sequence was cloned from K150009 by PCR.
mutable provenance: BBa_J23119, BBa_B0034 and K150009/BBa_K150007.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ColE1 immunity protein
sequence annotation: Kil protein fragment
sequence annotation: ColE1 protein
sequence: https://synbiohub.org/public/igem/BBa_K822002_sequence/1

ColE1 immunity protein
type: SequenceAnnotation
persistent identity: annotation2197334
display id: annotation2197334
version: 1
top level: BBa_K822002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K822002/annotation2197334/range2197334/1

https://synbiohub.org/public/igem/BBa_K822002/annotation2197334/range2197334/1
type: Range
persistent identity: range2197334
display id: range2197334
version: 1
direction: direction/reverse
top level: BBa_K822002
start: 1566
end: 1907
orientation: inline

Kil protein fragment
type: SequenceAnnotation
persistent identity: annotation2197335
display id: annotation2197335
version: 1
top level: BBa_K822002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K822002/annotation2197335/range2197335/1

https://synbiohub.org/public/igem/BBa_K822002/annotation2197335/range2197335/1
type: Range
persistent identity: range2197335
display id: range2197335
version: 1
direction: direction/forward
top level: BBa_K822002
start: 1955
end: 2050
orientation: inline

ColE1 protein
type: SequenceAnnotation
persistent identity: annotation2197333
display id: annotation2197333
version: 1
top level: BBa_K822002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K822002/annotation2197333/range2197333/1

https://synbiohub.org/public/igem/BBa_K822002/annotation2197333/range2197333/1
type: Range
persistent identity: range2197333
display id: range2197333
version: 1
direction: direction/forward
top level: BBa_K822002
start: 1
end: 1569
orientation: inline

BBa_I6064
type: ComponentDefinition
persistent identity: BBa I6064
display id: BBa_I6064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0050.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_E0032
component: BBa_B0010
component: BBa_B0034
component: BBa_R0050
sequence annotation: BBa_R0050
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I6064_sequence/1

BBa_B0012
type: Component
persistent identity: component949072
display id: component949072
version: 1
top level: BBa_I6064
access: public
definition: BBa_B0012

BBa_E0032
type: Component
persistent identity: component949054
display id: component949054
version: 1
top level: BBa_I6064
access: public
definition: YFP

BBa_B0010
type: Component
persistent identity: component949062
display id: component949062
version: 1
top level: BBa_I6064
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component949042
display id: component949042
version: 1
top level: BBa_I6064
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0050
type: Component
persistent identity: component949032
display id: component949032
version: 1
top level: BBa_I6064
access: public
definition: https://synbiohub.org/public/igem/BBa_R0050/1

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation949032
display id: annotation949032
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949032/range949032/1
component: BBa_R0050

https://synbiohub.org/public/igem/BBa_I6064/annotation949032/range949032/1
type: Range
persistent identity: range949032
display id: range949032
version: 1
direction: direction/forward
top level: BBa_I6064
start: 1
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949062
display id: annotation949062
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949062/range949062/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6064/annotation949062/range949062/1
type: Range
persistent identity: range949062
display id: range949062
version: 1
direction: direction/forward
top level: BBa_I6064
start: 852
end: 931
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949072
display id: annotation949072
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949072/range949072/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6064/annotation949072/range949072/1
type: Range
persistent identity: range949072
display id: range949072
version: 1
direction: direction/forward
top level: BBa_I6064
start: 940
end: 980
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949042
display id: annotation949042
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949042/range949042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6064/annotation949042/range949042/1
type: Range
persistent identity: range949042
display id: range949042
version: 1
direction: direction/forward
top level: BBa_I6064
start: 64
end: 75
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949054
display id: annotation949054
version: 1
top level: BBa_I6064
location: https://synbiohub.org/public/igem/BBa_I6064/annotation949054/range949054/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6064/annotation949054/range949054/1
type: Range
persistent identity: range949054
display id: range949054
version: 1
direction: direction/forward
top level: BBa_I6064
start: 82
end: 843
orientation: inline

BBa_K747042
type: ComponentDefinition
persistent identity: BBa K747042
display id: BBa_K747042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 3
sequence: https://synbiohub.org/public/igem/BBa_K747042_sequence/1

GG 3
type: SequenceAnnotation
persistent identity: annotation2196258
display id: annotation2196258
version: 1
top level: BBa_K747042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747042/annotation2196258/range2196258/1

https://synbiohub.org/public/igem/BBa_K747042/annotation2196258/range2196258/1
type: Range
persistent identity: range2196258
display id: range2196258
version: 1
direction: direction/forward
top level: BBa_K747042
start: 14
end: 211
orientation: inline

BBa_K929003
type: ComponentDefinition
persistent identity: BBa K929003
display id: BBa_K929003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified AID with CMV, hGH-polyA and eGFP
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14457
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K929001). It is built of 3 parts: CMV promoter (BBa_I712004), superAID, eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108). AID: AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes. The AID motif is naturally terminated with the Nuclear Export Sequence (NES) that causes the protein to translocate from the nucleus to the cytoplasm. Additionally, upstream, dysfunctional Nuclear Localization Sequence (NLS) is located. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate. That is why we called it superAID Functional NLS sequence: This part of the BioBrick directs the expressed protein into the nucleus, where it can mutate stronger. Kozak sequence Kozak consensus sequence is added upstream of the AID mutant to express the protein stronger. CMV promoter: CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter. hGH polyadenylation signal sequence: Polyadenylation is a significant part for the translation and stability of mRNA. In eukaryotes, it is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression. hGH terminator gives a signal to start polyadenylation in t…
mutable notes:
mutable provenance: 4 parts: CMV promoter (BBa_I712004), superAID(BBa_K929001), eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_I712004
sequence annotation: NLS sequence
sequence annotation: part of BBa_K103001
sequence annotation: UAA
sequence annotation: hGH terminator
sequence annotation: BBa_K929004
sequence annotation: BBa_K929001
sequence annotation: Kozak sequence
sequence annotation: AUG
sequence annotation: AgeI
sequence annotation: BBa_K404108
sequence annotation: BBa_K404316
sequence: https://synbiohub.org/public/igem/BBa_K929003_sequence/1

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2203637
display id: annotation2203637
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203637/range2203637/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203637/range2203637/1
type: Range
persistent identity: range2203637
display id: range2203637
version: 1
direction: direction/reverse
top level: BBa_K929003
start: 1
end: 654
orientation: inline

NLS sequence
type: SequenceAnnotation
persistent identity: annotation2203642
display id: annotation2203642
version: 1
top level: BBa_K929003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203642/range2203642/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203642/range2203642/1
type: Range
persistent identity: range2203642
display id: range2203642
version: 1
direction: direction/forward
top level: BBa_K929003
start: 678
end: 696
orientation: inline

part of BBa_K103001
type: SequenceAnnotation
persistent identity: annotation2203643
display id: annotation2203643
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203643/range2203643/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203643/range2203643/1
type: Range
persistent identity: range2203643
display id: range2203643
version: 1
direction: direction/forward
top level: BBa_K929003
start: 697
end: 1243
orientation: inline

UAA
type: SequenceAnnotation
persistent identity: annotation2203646
display id: annotation2203646
version: 1
top level: BBa_K929003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203646/range2203646/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203646/range2203646/1
type: Range
persistent identity: range2203646
display id: range2203646
version: 1
direction: direction/forward
top level: BBa_K929003
start: 1964
end: 1966
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2203647
display id: annotation2203647
version: 1
top level: BBa_K929003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203647/range2203647/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203647/range2203647/1
type: Range
persistent identity: range2203647
display id: range2203647
version: 1
direction: direction/forward
top level: BBa_K929003
start: 1975
end: 2453
orientation: inline

BBa_K929004
type: SequenceAnnotation
persistent identity: annotation2203640
display id: annotation2203640
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203640/range2203640/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203640/range2203640/1
type: Range
persistent identity: range2203640
display id: range2203640
version: 1
direction: direction/reverse
top level: BBa_K929003
start: 663
end: 1966
orientation: inline

BBa_K929001
type: SequenceAnnotation
persistent identity: annotation2203639
display id: annotation2203639
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203639/range2203639/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203639/range2203639/1
type: Range
persistent identity: range2203639
display id: range2203639
version: 1
direction: direction/forward
top level: BBa_K929003
start: 663
end: 1243
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2203638
display id: annotation2203638
version: 1
top level: BBa_K929003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203638/range2203638/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203638/range2203638/1
type: Range
persistent identity: range2203638
display id: range2203638
version: 1
direction: direction/forward
top level: BBa_K929003
start: 663
end: 677
orientation: inline

AUG
type: SequenceAnnotation
persistent identity: annotation2203641
display id: annotation2203641
version: 1
top level: BBa_K929003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203641/range2203641/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203641/range2203641/1
type: Range
persistent identity: range2203641
display id: range2203641
version: 1
direction: direction/forward
top level: BBa_K929003
start: 671
end: 673
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2203645
display id: annotation2203645
version: 1
top level: BBa_K929003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203645/range2203645/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203645/range2203645/1
type: Range
persistent identity: range2203645
display id: range2203645
version: 1
direction: direction/forward
top level: BBa_K929003
start: 1958
end: 1963
orientation: inline

BBa_K404108
type: SequenceAnnotation
persistent identity: annotation2203648
display id: annotation2203648
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203648/range2203648/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203648/range2203648/1
type: Range
persistent identity: range2203648
display id: range2203648
version: 1
direction: direction/reverse
top level: BBa_K929003
start: 1975
end: 2453
orientation: inline

BBa_K404316
type: SequenceAnnotation
persistent identity: annotation2203644
display id: annotation2203644
version: 1
top level: BBa_K929003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929003/annotation2203644/range2203644/1

https://synbiohub.org/public/igem/BBa_K929003/annotation2203644/range2203644/1
type: Range
persistent identity: range2203644
display id: range2203644
version: 1
direction: direction/forward
top level: BBa_K929003
start: 1244
end: 1966
orientation: inline

fhlA
type: ComponentDefinition
persistent identity: BBa K778001
display id: BBa_K778001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fhlA: a transcriptional activator for enhanced hydrogen production
creator: Tomohito Minakuchi
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: fhlA
mutable notes: pst1
mutable provenance: Escherichia coli (JM109)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A to G (1906bp) (different from E. coli K-12 MG1655)
sequence annotation: Illegal Pst1_site deletion (1854bp)
sequence annotation: stop
sequence annotation: start
sequence annotation: FhlA protein
sequence: https://synbiohub.org/public/igem/BBa_K778001_sequence/1

A to G (1906bp) (different from E. coli K-12 MG1655)
type: SequenceAnnotation
persistent identity: annotation2200693
display id: annotation2200693
version: 1
top level: fhlA
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2200693/range2200693/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2200693/range2200693/1
type: Range
persistent identity: range2200693
display id: range2200693
version: 1
direction: direction/forward
top level: fhlA
start: 1906
end: 1906
orientation: inline

Illegal Pst1_site deletion (1854bp)
type: SequenceAnnotation
persistent identity: annotation2200692
display id: annotation2200692
version: 1
top level: fhlA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2200692/range2200692/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2200692/range2200692/1
type: Range
persistent identity: range2200692
display id: range2200692
version: 1
direction: direction/forward
top level: fhlA
start: 1854
end: 1854
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2200694
display id: annotation2200694
version: 1
top level: fhlA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2200694/range2200694/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2200694/range2200694/1
type: Range
persistent identity: range2200694
display id: range2200694
version: 1
direction: direction/forward
top level: fhlA
start: 2077
end: 2079
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2200691
display id: annotation2200691
version: 1
top level: fhlA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2200691/range2200691/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2200691/range2200691/1
type: Range
persistent identity: range2200691
display id: range2200691
version: 1
direction: direction/forward
top level: fhlA
start: 1
end: 1
orientation: inline

FhlA protein
type: SequenceAnnotation
persistent identity: annotation2201429
display id: annotation2201429
version: 1
top level: fhlA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K778001/annotation2201429/range2201429/1

https://synbiohub.org/public/igem/BBa_K778001/annotation2201429/range2201429/1
type: Range
persistent identity: range2201429
display id: range2201429
version: 1
direction: direction/forward
top level: fhlA
start: 1
end: 2079
orientation: inline

BBa_K861031
type: ComponentDefinition
persistent identity: BBa K861031
display id: BBa_K861031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FadJ, gene for fatty acid degradation from E.coli str. K12
creator: Kuanwei Sheng
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FadJ is the anaerobic counterpart of FadB. The protein is shown to have better degradation of medium and short length fatty acid together with FadI.
mutable notes: None
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K861031_sequence/1

BBa_K801072
type: ComponentDefinition
persistent identity: BBa K801072
display id: BBa_K801072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB084125 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: Consensus sequence
sequence annotation: Strep Tag
sequence annotation: CaDXMT1
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801072_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198588
display id: annotation2198588
version: 1
top level: BBa_K801072
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1
type: Range
persistent identity: range2198588
display id: range2198588
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1189
end: 1194
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197893
display id: annotation2197893
version: 1
top level: BBa_K801072
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1
type: Range
persistent identity: range2197893
display id: range2197893
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1
end: 6
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198187
display id: annotation2198187
version: 1
top level: BBa_K801072
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1
type: Range
persistent identity: range2198187
display id: range2198187
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1159
end: 1188
orientation: inline

CaDXMT1
type: SequenceAnnotation
persistent identity: annotation2197894
display id: annotation2197894
version: 1
top level: BBa_K801072
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1
type: Range
persistent identity: range2197894
display id: range2197894
version: 1
direction: direction/forward
top level: BBa_K801072
start: 7
end: 1158
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198600
display id: annotation2198600
version: 1
top level: BBa_K801072
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1
type: Range
persistent identity: range2198600
display id: range2198600
version: 1
direction: direction/forward
top level: BBa_K801072
start: 7
end: 9
orientation: inline

BBa_K747007
type: ComponentDefinition
persistent identity: BBa K747007
display id: BBa_K747007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 1
sequence: https://synbiohub.org/public/igem/BBa_K747007_sequence/1

CT 1
type: SequenceAnnotation
persistent identity: annotation2195816
display id: annotation2195816
version: 1
top level: BBa_K747007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747007/annotation2195816/range2195816/1

https://synbiohub.org/public/igem/BBa_K747007/annotation2195816/range2195816/1
type: Range
persistent identity: range2195816
display id: range2195816
version: 1
direction: direction/forward
top level: BBa_K747007
start: 12
end: 209
orientation: inline

BBa_K747058
type: ComponentDefinition
persistent identity: BBa K747058
display id: BBa_K747058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 4
sequence: https://synbiohub.org/public/igem/BBa_K747058_sequence/1

GG 4
type: SequenceAnnotation
persistent identity: annotation2197015
display id: annotation2197015
version: 1
top level: BBa_K747058
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747058/annotation2197015/range2197015/1

https://synbiohub.org/public/igem/BBa_K747058/annotation2197015/range2197015/1
type: Range
persistent identity: range2197015
display id: range2197015
version: 1
direction: direction/forward
top level: BBa_K747058
start: 14
end: 211
orientation: inline

BBa_K747045
type: ComponentDefinition
persistent identity: BBa K747045
display id: BBa_K747045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 3
sequence: https://synbiohub.org/public/igem/BBa_K747045_sequence/1

TC 3
type: SequenceAnnotation
persistent identity: annotation2196264
display id: annotation2196264
version: 1
top level: BBa_K747045
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747045/annotation2196264/range2196264/1

https://synbiohub.org/public/igem/BBa_K747045/annotation2196264/range2196264/1
type: Range
persistent identity: range2196264
display id: range2196264
version: 1
direction: direction/forward
top level: BBa_K747045
start: 14
end: 211
orientation: inline

BBa_K917014
type: ComponentDefinition
persistent identity: BBa K917014
display id: BBa_K917014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac promoter plus S. oneidensis tetraheme cytochrome c cymA
creator: Jakub Krakowiak
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1182_
m user id: 0
owner id: 13804
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part consists of the standard registry lac promoter (BBa_J33207) S. oneidensis tetraheme cytochrome c cymA (K917009).
mutable notes: no special notes
mutable provenance: pLac gene comes from E. coli BL21 genomic DNA cymA was cloned from S. oneidensis MR-1 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J33207
component: BBa_K917009
sequence annotation: BBa_J33207
sequence annotation: BBa_K917009
sequence: https://synbiohub.org/public/igem/BBa_K917014_sequence/1

BBa_J33207
type: Component
persistent identity: component2196015
display id: component2196015
version: 1
top level: BBa_K917014
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_K917009
type: Component
persistent identity: component2196019
display id: component2196019
version: 1
top level: BBa_K917014
access: public
definition: https://synbiohub.org/public/igem/BBa_K917009/1

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2196015
display id: annotation2196015
version: 1
top level: BBa_K917014
location: https://synbiohub.org/public/igem/BBa_K917014/annotation2196015/range2196015/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K917014/annotation2196015/range2196015/1
type: Range
persistent identity: range2196015
display id: range2196015
version: 1
direction: direction/reverse
top level: BBa_K917014
start: 1
end: 600
orientation: inline

BBa_K917009
type: SequenceAnnotation
persistent identity: annotation2196019
display id: annotation2196019
version: 1
top level: BBa_K917014
location: https://synbiohub.org/public/igem/BBa_K917014/annotation2196019/range2196019/1
component: BBa_K917009

https://synbiohub.org/public/igem/BBa_K917014/annotation2196019/range2196019/1
type: Range
persistent identity: range2196019
display id: range2196019
version: 1
direction: direction/reverse
top level: BBa_K917014
start: 609
end: 1207
orientation: inline

BBa_K808033
type: ComponentDefinition
persistent identity: BBa K808033
display id: BBa_K808033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad regulated XylE - catechol 2,3-dioxygenase from P.putida with terminator
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K808000
component: BBa_J61100
component: BBa_K316003
sequence annotation: BBa_J61100
sequence annotation: BBa_K808000
sequence annotation: BBa_K316003
sequence: https://synbiohub.org/public/igem/BBa_K808033_sequence/1

BBa_K808000
type: Component
persistent identity: component2201322
display id: component2201322
version: 1
top level: BBa_K808033
access: public
definition: Ara

BBa_J61100
type: Component
persistent identity: component2201323
display id: component2201323
version: 1
top level: BBa_K808033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K316003
type: Component
persistent identity: component2201335
display id: component2201335
version: 1
top level: BBa_K808033
access: public
definition: https://synbiohub.org/public/igem/BBa_K316003/1

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201323
display id: annotation2201323
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201323/range2201323/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808033/annotation2201323/range2201323/1
type: Range
persistent identity: range2201323
display id: range2201323
version: 1
direction: direction/reverse
top level: BBa_K808033
start: 1218
end: 1229
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2201322
display id: annotation2201322
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201322/range2201322/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K808033/annotation2201322/range2201322/1
type: Range
persistent identity: range2201322
display id: range2201322
version: 1
direction: direction/reverse
top level: BBa_K808033
start: 1
end: 1209
orientation: inline

BBa_K316003
type: SequenceAnnotation
persistent identity: annotation2201335
display id: annotation2201335
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201335/range2201335/1
component: BBa_K316003

https://synbiohub.org/public/igem/BBa_K808033/annotation2201335/range2201335/1
type: Range
persistent identity: range2201335
display id: range2201335
version: 1
direction: direction/reverse
top level: BBa_K808033
start: 1238
end: 2298
orientation: inline

BBa_K952003
type: ComponentDefinition
persistent identity: BBa K952003
display id: BBa_K952003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-YF1-B0034-FixJ-PfixK2-amilGFP
creator: Saimon Sharif
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1220_
m user id: 0
owner id: 13332
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a composite of BBa_K592016, BBa_K592006, and BBa_K592010 in that order. When the cells are exposed to blue-light, they will express amilGFP, a yellow chromoprotein. This protein causes the cells to have a strong yellow color.
mutable notes: N/A
mutable provenance: BBa_K592016, BBa_K592006, and BBa_K592010 are available in the parts registry. They were obtained directly from the Uppsala-Sweden 2011 team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K592010
component: BBa_K592006
component: BBa_K592016
sequence annotation: BBa_K592006
sequence annotation: BBa_K592016
sequence annotation: BBa_K592010
sequence: https://synbiohub.org/public/igem/BBa_K952003_sequence/1

BBa_K592010
type: Component
persistent identity: component2205331
display id: component2205331
version: 1
top level: BBa_K952003
access: public
definition: https://synbiohub.org/public/igem/BBa_K592010/1

BBa_K592006
type: Component
persistent identity: component2205329
display id: component2205329
version: 1
top level: BBa_K952003
access: public
definition: https://synbiohub.org/public/igem/BBa_K592006/1

BBa_K592016
type: Component
persistent identity: component2205327
display id: component2205327
version: 1
top level: BBa_K952003
access: public
definition: https://synbiohub.org/public/igem/BBa_K592016/1

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2205329
display id: annotation2205329
version: 1
top level: BBa_K952003
location: https://synbiohub.org/public/igem/BBa_K952003/annotation2205329/range2205329/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_K952003/annotation2205329/range2205329/1
type: Range
persistent identity: range2205329
display id: range2205329
version: 1
direction: direction/reverse
top level: BBa_K952003
start: 1805
end: 2054
orientation: inline

BBa_K592016
type: SequenceAnnotation
persistent identity: annotation2205327
display id: annotation2205327
version: 1
top level: BBa_K952003
location: https://synbiohub.org/public/igem/BBa_K952003/annotation2205327/range2205327/1
component: BBa_K592016

https://synbiohub.org/public/igem/BBa_K952003/annotation2205327/range2205327/1
type: Range
persistent identity: range2205327
display id: range2205327
version: 1
direction: direction/reverse
top level: BBa_K952003
start: 1
end: 1796
orientation: inline

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2205331
display id: annotation2205331
version: 1
top level: BBa_K952003
location: https://synbiohub.org/public/igem/BBa_K952003/annotation2205331/range2205331/1
component: BBa_K592010

https://synbiohub.org/public/igem/BBa_K952003/annotation2205331/range2205331/1
type: Range
persistent identity: range2205331
display id: range2205331
version: 1
direction: direction/reverse
top level: BBa_K952003
start: 2061
end: 2759
orientation: inline

BBa_K801070
type: ComponentDefinition
persistent identity: BBa K801070
display id: BBa_K801070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xanthosine methyltransferase CaXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: xanthosine methyltransferase CaXMT1-strep from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB048793 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CaXMT1
sequence annotation: Consensus sequence
sequence annotation: Stop codon
sequence annotation: Start codon
sequence annotation: Strep Tag
sequence: https://synbiohub.org/public/igem/BBa_K801070_sequence/1

CaXMT1
type: SequenceAnnotation
persistent identity: annotation2198398
display id: annotation2198398
version: 1
top level: BBa_K801070
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1
type: Range
persistent identity: range2198398
display id: range2198398
version: 1
direction: direction/forward
top level: BBa_K801070
start: 7
end: 1122
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2198397
display id: annotation2198397
version: 1
top level: BBa_K801070
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1
type: Range
persistent identity: range2198397
display id: range2198397
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1
end: 6
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198401
display id: annotation2198401
version: 1
top level: BBa_K801070
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1
type: Range
persistent identity: range2198401
display id: range2198401
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1153
end: 1158
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198399
display id: annotation2198399
version: 1
top level: BBa_K801070
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1
type: Range
persistent identity: range2198399
display id: range2198399
version: 1
direction: direction/forward
top level: BBa_K801070
start: 7
end: 9
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198400
display id: annotation2198400
version: 1
top level: BBa_K801070
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1
type: Range
persistent identity: range2198400
display id: range2198400
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1123
end: 1152
orientation: inline

BBa_I6060
type: ComponentDefinition
persistent identity: BBa I6060
display id: BBa_I6060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0010.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0010
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_E0032
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6060_sequence/1

BBa_R0010
type: Component
persistent identity: component948886
display id: component948886
version: 1
top level: BBa_I6060
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component948916
display id: component948916
version: 1
top level: BBa_I6060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component948926
display id: component948926
version: 1
top level: BBa_I6060
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component948896
display id: component948896
version: 1
top level: BBa_I6060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component948908
display id: component948908
version: 1
top level: BBa_I6060
access: public
definition: YFP

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948896
display id: annotation948896
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948896/range948896/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6060/annotation948896/range948896/1
type: Range
persistent identity: range948896
display id: range948896
version: 1
direction: direction/forward
top level: BBa_I6060
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948926
display id: annotation948926
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948926/range948926/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6060/annotation948926/range948926/1
type: Range
persistent identity: range948926
display id: range948926
version: 1
direction: direction/forward
top level: BBa_I6060
start: 1085
end: 1125
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation948886
display id: annotation948886
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948886/range948886/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I6060/annotation948886/range948886/1
type: Range
persistent identity: range948886
display id: range948886
version: 1
direction: direction/forward
top level: BBa_I6060
start: 1
end: 200
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation948908
display id: annotation948908
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948908/range948908/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6060/annotation948908/range948908/1
type: Range
persistent identity: range948908
display id: range948908
version: 1
direction: direction/forward
top level: BBa_I6060
start: 227
end: 988
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948916
display id: annotation948916
version: 1
top level: BBa_I6060
location: https://synbiohub.org/public/igem/BBa_I6060/annotation948916/range948916/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6060/annotation948916/range948916/1
type: Range
persistent identity: range948916
display id: range948916
version: 1
direction: direction/forward
top level: BBa_I6060
start: 997
end: 1076
orientation: inline

otsA
type: ComponentDefinition
persistent identity: BBa K847060
display id: BBa_K847060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Escherichia coli'' osmoregulatory trehalose synthesis A (otsA)
creator: Rashmi Sharma, Vishesh Jain, Kendrick Wang
created: 2012-08-08T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1107_
m user id: 0
owner id: 11092
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tba
mutable notes: tba
mutable provenance: tba
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K847060_sequence/1

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0032.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0032
component: BBa_E0040
component: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
access: public
definition: BBa_B0012

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
access: public
definition: BBa_B0032

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
direction: direction/forward
top level: GFP report
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
direction: direction/forward
top level: GFP report
start: 748
end: 827
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
direction: direction/forward
top level: GFP report
start: 1
end: 13
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
direction: direction/forward
top level: GFP report
start: 836
end: 876
orientation: inline

BBa_K747010
type: ComponentDefinition
persistent identity: BBa K747010
display id: BBa_K747010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 1
sequence: https://synbiohub.org/public/igem/BBa_K747010_sequence/1

GG 1
type: SequenceAnnotation
persistent identity: annotation2195829
display id: annotation2195829
version: 1
top level: BBa_K747010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747010/annotation2195829/range2195829/1

https://synbiohub.org/public/igem/BBa_K747010/annotation2195829/range2195829/1
type: Range
persistent identity: range2195829
display id: range2195829
version: 1
direction: direction/forward
top level: BBa_K747010
start: 12
end: 209
orientation: inline

Cel5A
type: ComponentDefinition
persistent identity: BBa K805011
display id: BBa_K805011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cel5A, endoglucanases
creator: Liu Ping
created: 2012-09-19T11:00:00Z
modified: 2015-06-19T01:48:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1062_
m user id: 4206
owner id: 11895
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: test
mutable notes: test
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K805011_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2200607
display id: annotation2200607
version: 1
top level: Cel5A
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K805011/annotation2200607/range2200607/1

https://synbiohub.org/public/igem/BBa_K805011/annotation2200607/range2200607/1
type: Range
persistent identity: range2200607
display id: range2200607
version: 1
direction: direction/forward
top level: Cel5A
start: 1
end: 3
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation2200608
display id: annotation2200608
version: 1
top level: Cel5A
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K805011/annotation2200608/range2200608/1

https://synbiohub.org/public/igem/BBa_K805011/annotation2200608/range2200608/1
type: Range
persistent identity: range2200608
display id: range2200608
version: 1
direction: direction/forward
top level: Cel5A
start: 4
end: 1233
orientation: inline

BBa_K895005
type: ComponentDefinition
persistent identity: BBa K895005
display id: BBa_K895005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salty_VgrG-CD27_endolysin
creator: Frank Sargent
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:41Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1160_
m user id: 0
owner id: 8083
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a coding part comprising the full open reading frame of Salmonella enterica LT2 vgrG gene (also known as STM0289 or tssI) linked via an haemagglutinin eptitope tag to a synthetic gene encoding an endolysin from the CD27 bacteriophage. The CD27 endolysin is believed to have high specificity against the cell wall of Clostridium difficile.
mutable notes: None.
mutable provenance: The vgrG section is derived from BBa_K895002 , which was used as a PCR template, and the endolysin section is derived from a synthetic gene designed by Neil Fairweather's group at Imperial College London.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HA tag
sequence annotation: endolysin
sequence annotation: vgrG
sequence: https://synbiohub.org/public/igem/BBa_K895005_sequence/1

HA tag
type: SequenceAnnotation
persistent identity: annotation2194045
display id: annotation2194045
version: 1
top level: BBa_K895005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K895005/annotation2194045/range2194045/1

https://synbiohub.org/public/igem/BBa_K895005/annotation2194045/range2194045/1
type: Range
persistent identity: range2194045
display id: range2194045
version: 1
direction: direction/forward
top level: BBa_K895005
start: 2188
end: 2214
orientation: inline

endolysin
type: SequenceAnnotation
persistent identity: annotation2194046
display id: annotation2194046
version: 1
top level: BBa_K895005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895005/annotation2194046/range2194046/1

https://synbiohub.org/public/igem/BBa_K895005/annotation2194046/range2194046/1
type: Range
persistent identity: range2194046
display id: range2194046
version: 1
direction: direction/forward
top level: BBa_K895005
start: 2221
end: 2793
orientation: inline

vgrG
type: SequenceAnnotation
persistent identity: annotation2194044
display id: annotation2194044
version: 1
top level: BBa_K895005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895005/annotation2194044/range2194044/1

https://synbiohub.org/public/igem/BBa_K895005/annotation2194044/range2194044/1
type: Range
persistent identity: range2194044
display id: range2194044
version: 1
direction: direction/forward
top level: BBa_K895005
start: 1
end: 2187
orientation: inline

BBa_I6030
type: ComponentDefinition
persistent identity: BBa I6030
display id: BBa_I6030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0010.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_R0010
component: BBa_E0030
sequence annotation: BBa_E0030
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6030_sequence/1

BBa_B0012
type: Component
persistent identity: component948302
display id: component948302
version: 1
top level: BBa_I6030
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component948285
display id: component948285
version: 1
top level: BBa_I6030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component948292
display id: component948292
version: 1
top level: BBa_I6030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component948275
display id: component948275
version: 1
top level: BBa_I6030
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0030
type: Component
persistent identity: component948287
display id: component948287
version: 1
top level: BBa_I6030
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948287
display id: annotation948287
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948287/range948287/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6030/annotation948287/range948287/1
type: Range
persistent identity: range948287
display id: range948287
version: 1
direction: direction/forward
top level: BBa_I6030
start: 227
end: 949
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation948275
display id: annotation948275
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948275/range948275/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I6030/annotation948275/range948275/1
type: Range
persistent identity: range948275
display id: range948275
version: 1
direction: direction/forward
top level: BBa_I6030
start: 1
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948302
display id: annotation948302
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948302/range948302/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6030/annotation948302/range948302/1
type: Range
persistent identity: range948302
display id: range948302
version: 1
direction: direction/forward
top level: BBa_I6030
start: 1046
end: 1086
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948285
display id: annotation948285
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948285/range948285/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6030/annotation948285/range948285/1
type: Range
persistent identity: range948285
display id: range948285
version: 1
direction: direction/forward
top level: BBa_I6030
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948292
display id: annotation948292
version: 1
top level: BBa_I6030
location: https://synbiohub.org/public/igem/BBa_I6030/annotation948292/range948292/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6030/annotation948292/range948292/1
type: Range
persistent identity: range948292
display id: range948292
version: 1
direction: direction/forward
top level: BBa_I6030
start: 958
end: 1037
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.4 (weaker) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
direction: direction/forward
top level: BBa_B0033
start: 7
end: 10
orientation: inline

BBa_I6108
type: ComponentDefinition
persistent identity: BBa I6108
display id: BBa_I6108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0080.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_R0080
component: BBa_B0034
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_R0080
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6108_sequence/1

BBa_B0010
type: Component
persistent identity: component949969
display id: component949969
version: 1
top level: BBa_I6108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0080
type: Component
persistent identity: component949957
display id: component949957
version: 1
top level: BBa_I6108
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_B0034
type: Component
persistent identity: component949962
display id: component949962
version: 1
top level: BBa_I6108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component949979
display id: component949979
version: 1
top level: BBa_I6108
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component949964
display id: component949964
version: 1
top level: BBa_I6108
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation949957
display id: annotation949957
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949957/range949957/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_I6108/annotation949957/range949957/1
type: Range
persistent identity: range949957
display id: range949957
version: 1
direction: direction/forward
top level: BBa_I6108
start: 1
end: 149
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949969
display id: annotation949969
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949969/range949969/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6108/annotation949969/range949969/1
type: Range
persistent identity: range949969
display id: range949969
version: 1
direction: direction/forward
top level: BBa_I6108
start: 907
end: 986
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949964
display id: annotation949964
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949964/range949964/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6108/annotation949964/range949964/1
type: Range
persistent identity: range949964
display id: range949964
version: 1
direction: direction/forward
top level: BBa_I6108
start: 176
end: 898
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949979
display id: annotation949979
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949979/range949979/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6108/annotation949979/range949979/1
type: Range
persistent identity: range949979
display id: range949979
version: 1
direction: direction/forward
top level: BBa_I6108
start: 995
end: 1035
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949962
display id: annotation949962
version: 1
top level: BBa_I6108
location: https://synbiohub.org/public/igem/BBa_I6108/annotation949962/range949962/1
component: BBa_B0034

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type: Range
persistent identity: range949962
display id: range949962
version: 1
direction: direction/forward
top level: BBa_I6108
start: 158
end: 169
orientation: inline

ArgBox16
type: ComponentDefinition
persistent identity: BBa K778006
display id: BBa_K778006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ArgR binding sites (including 16 binding sites)
creator: Yuka Yamazaki
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K778003 ??2
mutable notes: sukima
mutable provenance: biobrick part (BBa_K778003)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_K778003
sequence annotation: BBa_K778003
sequence: https://synbiohub.org/public/igem/BBa_K778006_sequence/1

BBa_K778003
type: Component
persistent identity: component2194871
display id: component2194871
version: 1
top level: ArgBox16
access: public
definition: https://synbiohub.org/public/igem/BBa_K778003/1

BBa_K778003
type: Component
persistent identity: component2194872
display id: component2194872
version: 1
top level: ArgBox16
access: public
definition: https://synbiohub.org/public/igem/BBa_K778003/1

BBa_K778003
type: SequenceAnnotation
persistent identity: annotation2194871
display id: annotation2194871
version: 1
top level: ArgBox16
location: https://synbiohub.org/public/igem/BBa_K778006/annotation2194871/range2194871/1
component: BBa_K778003

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version: 1
direction: direction/reverse
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start: 1
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orientation: inline

BBa_K778003
type: SequenceAnnotation
persistent identity: annotation2194872
display id: annotation2194872
version: 1
top level: ArgBox16
location: https://synbiohub.org/public/igem/BBa_K778006/annotation2194872/range2194872/1
component: BBa_K778003

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type: Range
persistent identity: range2194872
display id: range2194872
version: 1
direction: direction/reverse
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start: 153
end: 296
orientation: inline

BBa_K773004
type: ComponentDefinition
persistent identity: BBa K773004
display id: BBa_K773004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-AAV
creator: Katie Knister
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part holds the fluorescent protein mCherry attached to a degradation tag AAV which breaks down fluorescent protein over time.
mutable notes: We eliminated a restriction site from the gene.
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Stop
sequence annotation: ssrA degradation tag: AAV
sequence annotation: mCherry-AAV
sequence annotation: B0034
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K773004_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2209504
display id: annotation2209504
version: 1
top level: BBa_K773004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209504/range2209504/1

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persistent identity: range2209504
display id: range2209504
version: 1
direction: direction/forward
top level: BBa_K773004
start: 760
end: 762
orientation: inline

ssrA degradation tag: AAV
type: SequenceAnnotation
persistent identity: annotation2209506
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version: 1
top level: BBa_K773004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209506/range2209506/1

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display id: range2209506
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direction: direction/forward
top level: BBa_K773004
start: 730
end: 759
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209505
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version: 1
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role: SO:0000319
role: feature/stop
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persistent identity: range2209505
display id: range2209505
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direction: direction/forward
top level: BBa_K773004
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end: 765
orientation: inline

mCherry-AAV
type: SequenceAnnotation
persistent identity: annotation2209503
display id: annotation2209503
version: 1
top level: BBa_K773004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209503/range2209503/1

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display id: range2209503
version: 1
direction: direction/forward
top level: BBa_K773004
start: 19
end: 765
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2209502
display id: annotation2209502
version: 1
top level: BBa_K773004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209502/range2209502/1

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type: Range
persistent identity: range2209502
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version: 1
direction: direction/forward
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start: 1
end: 12
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209507
display id: annotation2209507
version: 1
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role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K773004/annotation2209507/range2209507/1

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type: Range
persistent identity: range2209507
display id: range2209507
version: 1
direction: direction/forward
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start: 19
end: 21
orientation: inline

BBa_I6106
type: ComponentDefinition
persistent identity: BBa I6106
display id: BBa_I6106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0078.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_E0030
component: BBa_R0078
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_R0078
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6106_sequence/1

BBa_B0012
type: Component
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display id: component949907
version: 1
top level: BBa_I6106
access: public
definition: BBa_B0012

BBa_E0030
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display id: component949892
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0078
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display id: component949885
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0078/1

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display id: component949897
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component949890
display id: component949890
version: 1
top level: BBa_I6106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation949885
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version: 1
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component: BBa_R0078

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orientation: inline

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direction: direction/forward
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orientation: inline

BBa_B0012
type: SequenceAnnotation
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display id: annotation949907
version: 1
top level: BBa_I6106
location: https://synbiohub.org/public/igem/BBa_I6106/annotation949907/range949907/1
component: BBa_B0012

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persistent identity: range949907
display id: range949907
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direction: direction/forward
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type: SequenceAnnotation
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version: 1
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persistent identity: range949892
display id: range949892
version: 1
direction: direction/forward
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start: 252
end: 974
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949890
display id: annotation949890
version: 1
top level: BBa_I6106
location: https://synbiohub.org/public/igem/BBa_I6106/annotation949890/range949890/1
component: BBa_B0034

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type: Range
persistent identity: range949890
display id: range949890
version: 1
direction: direction/forward
top level: BBa_I6106
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end: 245
orientation: inline

BBa_I6046
type: ComponentDefinition
persistent identity: BBa I6046
display id: BBa_I6046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0065.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_E0030
component: BBa_R0065
sequence annotation: BBa_B0010
sequence annotation: BBa_R0065
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6046_sequence/1

BBa_B0034
type: Component
persistent identity: component948620
display id: component948620
version: 1
top level: BBa_I6046
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component948637
display id: component948637
version: 1
top level: BBa_I6046
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component948627
display id: component948627
version: 1
top level: BBa_I6046
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component948622
display id: component948622
version: 1
top level: BBa_I6046
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0065
type: Component
persistent identity: component948608
display id: component948608
version: 1
top level: BBa_I6046
access: public
definition: cI+luxR

BBa_B0010
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type: Range
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display id: range948627
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direction: direction/forward
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orientation: inline

BBa_R0065
type: SequenceAnnotation
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display id: annotation948608
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948608/range948608/1
component: BBa_R0065

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persistent identity: range948608
display id: range948608
version: 1
direction: direction/forward
top level: BBa_I6046
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orientation: inline

BBa_E0030
type: SequenceAnnotation
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display id: annotation948622
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948622/range948622/1
component: BBa_E0030

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type: Range
persistent identity: range948622
display id: range948622
version: 1
direction: direction/forward
top level: BBa_I6046
start: 124
end: 846
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948620
display id: annotation948620
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948620/range948620/1
component: BBa_B0034

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type: Range
persistent identity: range948620
display id: range948620
version: 1
direction: direction/forward
top level: BBa_I6046
start: 106
end: 117
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948637
display id: annotation948637
version: 1
top level: BBa_I6046
location: https://synbiohub.org/public/igem/BBa_I6046/annotation948637/range948637/1
component: BBa_B0012

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type: Range
persistent identity: range948637
display id: range948637
version: 1
direction: direction/forward
top level: BBa_I6046
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end: 983
orientation: inline

BBa_S01002
type: ComponentDefinition
persistent identity: BBa S01002
display id: BBa_S01002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0071.E0422
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 Tube 46
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_E0022
component: BBa_R0071
component: BBa_B0034
component: BBa_B0012
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sequence annotation: BBa_B0010
sequence annotation: BBa_E0022
sequence annotation: BBa_R0071
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S01002_sequence/1

BBa_B0010
type: Component
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display id: component1511051
version: 1
top level: BBa_S01002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: Component
persistent identity: component1511043
display id: component1511043
version: 1
top level: BBa_S01002
access: public
definition: ECFP

BBa_R0071
type: Component
persistent identity: component1511026
display id: component1511026
version: 1
top level: BBa_S01002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0034
type: Component
persistent identity: component1511031
display id: component1511031
version: 1
top level: BBa_S01002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
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display id: component1511061
version: 1
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access: public
definition: BBa_B0012

BBa_B0034
type: SequenceAnnotation
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display id: annotation1511031
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type: Range
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display id: range1511031
version: 1
direction: direction/forward
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orientation: inline

BBa_B0010
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persistent identity: range1511051
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direction: direction/forward
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orientation: inline

BBa_E0022
type: SequenceAnnotation
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display id: annotation1511043
version: 1
top level: BBa_S01002
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display id: range1511043
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direction: direction/forward
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orientation: inline

BBa_R0071
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version: 1
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component: BBa_R0071

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direction: direction/forward
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BBa_B0012
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type: Range
persistent identity: range1511061
display id: range1511061
version: 1
direction: direction/forward
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orientation: inline

BBa_K902059
type: ComponentDefinition
persistent identity: BBa K902059
display id: BBa_K902059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: katG +LAA behind an RBS (B0034)
creator: Lisa Oberding, Ali Honarmand
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: KatG +LAA behind and RBS site (B0034)
mutable notes: None
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K137068
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K137068
sequence: https://synbiohub.org/public/igem/BBa_K902059_sequence/1

BBa_K137068
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top level: BBa_K902059
access: public
definition: https://synbiohub.org/public/igem/BBa_K137068/1

BBa_B0034
type: Component
persistent identity: component2205168
display id: component2205168
version: 1
top level: BBa_K902059
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2205168
display id: annotation2205168
version: 1
top level: BBa_K902059
location: https://synbiohub.org/public/igem/BBa_K902059/annotation2205168/range2205168/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K902059/annotation2205168/range2205168/1
type: Range
persistent identity: range2205168
display id: range2205168
version: 1
direction: direction/reverse
top level: BBa_K902059
start: 1
end: 12
orientation: inline

BBa_K137068
type: SequenceAnnotation
persistent identity: annotation2205170
display id: annotation2205170
version: 1
top level: BBa_K902059
location: https://synbiohub.org/public/igem/BBa_K902059/annotation2205170/range2205170/1
component: BBa_K137068

https://synbiohub.org/public/igem/BBa_K902059/annotation2205170/range2205170/1
type: Range
persistent identity: range2205170
display id: range2205170
version: 1
direction: direction/reverse
top level: BBa_K902059
start: 19
end: 2235
orientation: inline

BBa_K747035
type: ComponentDefinition
persistent identity: BBa K747035
display id: BBa_K747035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 3
sequence: https://synbiohub.org/public/igem/BBa_K747035_sequence/1

AT 3
type: SequenceAnnotation
persistent identity: annotation2196211
display id: annotation2196211
version: 1
top level: BBa_K747035
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747035/annotation2196211/range2196211/1

https://synbiohub.org/public/igem/BBa_K747035/annotation2196211/range2196211/1
type: Range
persistent identity: range2196211
display id: range2196211
version: 1
direction: direction/forward
top level: BBa_K747035
start: 14
end: 211
orientation: inline

BBa_I732805
type: ComponentDefinition
persistent identity: BBa I732805
display id: BBa_I732805
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0101 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_I732105
sequence annotation: BBa_B0034
sequence annotation: BBa_I732105
sequence: https://synbiohub.org/public/igem/BBa_I732805_sequence/1

BBa_B0034
type: Component
persistent identity: component1946177
display id: component1946177
version: 1
top level: BBa_I732805
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732105
type: Component
persistent identity: component1946178
display id: component1946178
version: 1
top level: BBa_I732805
access: public
definition: https://synbiohub.org/public/igem/BBa_I732105/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946177
display id: annotation1946177
version: 1
top level: BBa_I732805
location: https://synbiohub.org/public/igem/BBa_I732805/annotation1946177/range1946177/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732805/annotation1946177/range1946177/1
type: Range
persistent identity: range1946177
display id: range1946177
version: 1
direction: direction/reverse
top level: BBa_I732805
start: 1
end: 12
orientation: inline

BBa_I732105
type: SequenceAnnotation
persistent identity: annotation1946178
display id: annotation1946178
version: 1
top level: BBa_I732805
location: https://synbiohub.org/public/igem/BBa_I732805/annotation1946178/range1946178/1
component: BBa_I732105

https://synbiohub.org/public/igem/BBa_I732805/annotation1946178/range1946178/1
type: Range
persistent identity: range1946178
display id: range1946178
version: 1
direction: direction/reverse
top level: BBa_I732805
start: 19
end: 1104
orientation: inline

BBa_J202012
type: ComponentDefinition
persistent identity: BBa J202012
display id: BBa_J202012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#13
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -70 relative to the araC transcriptional start site was changed from G to A.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI1
sequence annotation: araI2
sequence annotation: Mutation
sequence annotation: Transcription Start
sequence: https://synbiohub.org/public/igem/BBa_J202012_sequence/1

araI1
type: SequenceAnnotation
persistent identity: annotation2178467
display id: annotation2178467
version: 1
top level: BBa_J202012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202012/annotation2178467/range2178467/1

https://synbiohub.org/public/igem/BBa_J202012/annotation2178467/range2178467/1
type: Range
persistent identity: range2178467
display id: range2178467
version: 1
direction: direction/forward
top level: BBa_J202012
start: 40
end: 56
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178468
display id: annotation2178468
version: 1
top level: BBa_J202012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202012/annotation2178468/range2178468/1

https://synbiohub.org/public/igem/BBa_J202012/annotation2178468/range2178468/1
type: Range
persistent identity: range2178468
display id: range2178468
version: 1
direction: direction/forward
top level: BBa_J202012
start: 61
end: 77
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178465
display id: annotation2178465
version: 1
top level: BBa_J202012
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202012/annotation2178465/range2178465/1

https://synbiohub.org/public/igem/BBa_J202012/annotation2178465/range2178465/1
type: Range
persistent identity: range2178465
display id: range2178465
version: 1
direction: direction/forward
top level: BBa_J202012
start: 42
end: 42
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178466
display id: annotation2178466
version: 1
top level: BBa_J202012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202012/annotation2178466/range2178466/1

https://synbiohub.org/public/igem/BBa_J202012/annotation2178466/range2178466/1
type: Range
persistent identity: range2178466
display id: range2178466
version: 1
direction: direction/forward
top level: BBa_J202012
start: 112
end: 112
orientation: inline

BBa_K863010
type: ComponentDefinition
persistent identity: BBa K863010
display id: BBa_K863010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T7 promoter
sequence annotation: 6x HIS
sequence annotation: ATG
sequence annotation: tthl
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863010_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184708
display id: annotation2184708
version: 1
top level: BBa_K863010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1
type: Range
persistent identity: range2184708
display id: range2184708
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1
end: 23
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184733
display id: annotation2184733
version: 1
top level: BBa_K863010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1
type: Range
persistent identity: range2184733
display id: range2184733
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1420
end: 1437
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184671
display id: annotation2184671
version: 1
top level: BBa_K863010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1
type: Range
persistent identity: range2184671
display id: range2184671
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 36
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2184732
display id: annotation2184732
version: 1
top level: BBa_K863010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1
type: Range
persistent identity: range2184732
display id: range2184732
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 1419
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184782
display id: annotation2184782
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1
type: Range
persistent identity: range2184782
display id: range2184782
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1438
end: 1440
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184783
display id: annotation2184783
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1
type: Range
persistent identity: range2184783
display id: range2184783
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1441
end: 1443
orientation: inline

BBa_P0152
type: ComponentDefinition
persistent identity: BBa P0152
display id: BBa_P0152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (434) [S0166]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from 434. Used as the input section for Quad Part Inverter Q01520.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0031
component: BBa_C0052
component: BBa_B0010
sequence annotation: BBa_C0052
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0152_sequence/1

BBa_B0012
type: Component
persistent identity: component944562
display id: component944562
version: 1
top level: BBa_P0152
access: public
definition: BBa_B0012

BBa_B0031
type: Component
persistent identity: component944535
display id: component944535
version: 1
top level: BBa_P0152
access: public
definition: BBa_B0031

BBa_C0052
type: Component
persistent identity: component944545
display id: component944545
version: 1
top level: BBa_P0152
access: public
definition: cI 434

BBa_B0010
type: Component
persistent identity: component944552
display id: component944552
version: 1
top level: BBa_P0152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation944545
display id: annotation944545
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
type: Range
persistent identity: range944545
display id: range944545
version: 1
direction: direction/forward
top level: BBa_P0152
start: 21
end: 689
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944562
display id: annotation944562
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
type: Range
persistent identity: range944562
display id: range944562
version: 1
direction: direction/forward
top level: BBa_P0152
start: 811
end: 851
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944552
display id: annotation944552
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
type: Range
persistent identity: range944552
display id: range944552
version: 1
direction: direction/forward
top level: BBa_P0152
start: 723
end: 802
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944535
display id: annotation944535
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
type: Range
persistent identity: range944535
display id: range944535
version: 1
direction: direction/forward
top level: BBa_P0152
start: 1
end: 14
orientation: inline

BBa_K747050
type: ComponentDefinition
persistent identity: BBa K747050
display id: BBa_K747050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 4
sequence: https://synbiohub.org/public/igem/BBa_K747050_sequence/1

AG 4
type: SequenceAnnotation
persistent identity: annotation2196279
display id: annotation2196279
version: 1
top level: BBa_K747050
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747050/annotation2196279/range2196279/1

https://synbiohub.org/public/igem/BBa_K747050/annotation2196279/range2196279/1
type: Range
persistent identity: range2196279
display id: range2196279
version: 1
direction: direction/forward
top level: BBa_K747050
start: 14
end: 211
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphageT7
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcription terminator for the E.coli RNA polymerase.
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
direction: direction/forward
top level: BBa_B0012
start: 1
end: 41
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
direction: direction/forward
top level: BBa_B0012
start: 28
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
direction: direction/forward
top level: BBa_B0012
start: 8
end: 27
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
direction: direction/forward
top level: BBa_B0012
start: 34
end: 34
orientation: inline

BBa_I9026
type: ComponentDefinition
persistent identity: BBa I9026
display id: BBa_I9026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlI translational unit
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0070
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_I9026_sequence/1

BBa_C0070
type: Component
persistent identity: component939656
display id: component939656
version: 1
top level: BBa_I9026
access: public
definition: https://synbiohub.org/public/igem/BBa_C0070/1

BBa_B0034
type: Component
persistent identity: component939646
display id: component939646
version: 1
top level: BBa_I9026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939646
display id: annotation939646
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
type: Range
persistent identity: range939646
display id: range939646
version: 1
direction: direction/forward
top level: BBa_I9026
start: 1
end: 12
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation939656
display id: annotation939656
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
type: Range
persistent identity: range939656
display id: range939656
version: 1
direction: direction/forward
top level: BBa_I9026
start: 19
end: 660
orientation: inline

BBa_K817000
type: ComponentDefinition
persistent identity: BBa K817000
display id: BBa_K817000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP1-GLP1
creator: Yen-Der Li
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1075_
m user id: 0
owner id: 13572
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the GLP-1 coding part along with the signal peptide sequence.
mutable notes: SP1-GLP1
mutable provenance: SP1-GLP1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GLP-1
sequence annotation: SP1
sequence: https://synbiohub.org/public/igem/BBa_K817000_sequence/1

GLP-1
type: SequenceAnnotation
persistent identity: annotation2201480
display id: annotation2201480
version: 1
top level: BBa_K817000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K817000/annotation2201480/range2201480/1

https://synbiohub.org/public/igem/BBa_K817000/annotation2201480/range2201480/1
type: Range
persistent identity: range2201480
display id: range2201480
version: 1
direction: direction/forward
top level: BBa_K817000
start: 65
end: 162
orientation: inline

SP1
type: SequenceAnnotation
persistent identity: annotation2201479
display id: annotation2201479
version: 1
top level: BBa_K817000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K817000/annotation2201479/range2201479/1

https://synbiohub.org/public/igem/BBa_K817000/annotation2201479/range2201479/1
type: Range
persistent identity: range2201479
display id: range2201479
version: 1
direction: direction/forward
top level: BBa_K817000
start: 1
end: 64
orientation: inline

phaC1-A-B1
type: ComponentDefinition
persistent identity: BBa K934001
display id: BBa_K934001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaC1-A-B1 [P(3HB) synthesis]
creator: Taku Nakayama
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1199_
m user id: 0
owner id: 14016
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The sequence comes from Ralstonia eutropha H16 adapted to become a standard BioBrick.
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RBS
sequence annotation: Pwt
sequence annotation: phaB1
sequence annotation: phaC1
sequence annotation: phaA
sequence: https://synbiohub.org/public/igem/BBa_K934001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2192331
display id: annotation2192331
version: 1
top level: phaC1-A-B1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192331/range2192331/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192331/range2192331/1
type: Range
persistent identity: range2192331
display id: range2192331
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 3453
end: 3456
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192329
display id: annotation2192329
version: 1
top level: phaC1-A-B1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192329/range2192329/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192329/range2192329/1
type: Range
persistent identity: range2192329
display id: range2192329
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 336
end: 342
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192330
display id: annotation2192330
version: 1
top level: phaC1-A-B1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192330/range2192330/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192330/range2192330/1
type: Range
persistent identity: range2192330
display id: range2192330
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 2196
end: 2199
orientation: inline

Pwt
type: SequenceAnnotation
persistent identity: annotation2192328
display id: annotation2192328
version: 1
top level: phaC1-A-B1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192328/range2192328/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192328/range2192328/1
type: Range
persistent identity: range2192328
display id: range2192328
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 1
end: 50
orientation: inline

phaB1
type: SequenceAnnotation
persistent identity: annotation2192327
display id: annotation2192327
version: 1
top level: phaC1-A-B1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192327/range2192327/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192327/range2192327/1
type: Range
persistent identity: range2192327
display id: range2192327
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 3463
end: 4203
orientation: inline

phaC1
type: SequenceAnnotation
persistent identity: annotation2192325
display id: annotation2192325
version: 1
top level: phaC1-A-B1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192325/range2192325/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192325/range2192325/1
type: Range
persistent identity: range2192325
display id: range2192325
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 353
end: 2122
orientation: inline

phaA
type: SequenceAnnotation
persistent identity: annotation2192326
display id: annotation2192326
version: 1
top level: phaC1-A-B1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192326/range2192326/1

https://synbiohub.org/public/igem/BBa_K934001/annotation2192326/range2192326/1
type: Range
persistent identity: range2192326
display id: range2192326
version: 1
direction: direction/forward
top level: phaC1-A-B1
start: 2207
end: 3388
orientation: inline

BBa_K876014
type: ComponentDefinition
persistent identity: BBa K876014
display id: BBa_K876014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-LuxI
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter for LuxI which can be repressed by LacI
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_C0061
sequence annotation: BBa_C0061
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K876014_sequence/1

BBa_R0010
type: Component
persistent identity: component2245106
display id: component2245106
version: 1
top level: BBa_K876014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0061
type: Component
persistent identity: component2245114
display id: component2245114
version: 1
top level: BBa_K876014
access: public
definition: luxI

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2245114
display id: annotation2245114
version: 1
top level: BBa_K876014
location: https://synbiohub.org/public/igem/BBa_K876014/annotation2245114/range2245114/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876014/annotation2245114/range2245114/1
type: Range
persistent identity: range2245114
display id: range2245114
version: 1
direction: direction/forward
top level: BBa_K876014
start: 207
end: 824
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2245106
display id: annotation2245106
version: 1
top level: BBa_K876014
location: https://synbiohub.org/public/igem/BBa_K876014/annotation2245106/range2245106/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876014/annotation2245106/range2245106/1
type: Range
persistent identity: range2245106
display id: range2245106
version: 1
direction: direction/forward
top level: BBa_K876014
start: 1
end: 200
orientation: inline

BBa_J61016
type: ComponentDefinition
persistent identity: BBa J61016
display id: BBa_J61016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biobrick converter for BamHI-part-EcoRI
creator: John Anderson
created: 2006-08-10T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inserting a BamHI/EcoRI fragment into the BglII/MfeI sites of J61015 destroys the restriction sites and introduces standard Biobrick flanking sequence. In the process, the OnRFP cassette is destroyed allowing Red to White screening of colonies.
mutable notes: N/A
mutable provenance: PCR ca1007F/R on pSB1A2-J01064 (972 bp, EcoRI/SpeI) PCR ca1008F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI) PCR ca1007F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI) Sub into pSB1A2 (EcoRI/SpeI) Products are: Bca1007 NotI/BamHI transfer Bca1008 BamHI/EcoRI transfer Bca1009 NotI/EcoRI transfer ------------------------------------------------ ca1007F Forward EcoRI for 5' PspOMI NotI converter ctctggaattcgcggccgcttctagagggccctccctatcagtgatagagattg ca1007R Reverse SpeI for 3' BglII BamHI converter ccgctactagtagatcttataaacgcagaaaggcccac ca1008F Forward EcoRI for 5' BglII BamHI converter ctctggaattcgcggccgcttctagaagatcttccctatcagtgatagagattg ca1009R Reverse SpeI for 3' MfeI EcoRI converter ccgctactagtcaattgtataaacgcagaaaggcccac
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J61016_sequence/1

BBa_K909009
type: ComponentDefinition
persistent identity: BBa K909009
display id: BBa_K909009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection. In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain), to use UVR8 as a UV-B sensing domain.
mutable notes: Contains two illegal PstI sites.
mutable provenance: cDNA of UVR8 from Arabidopsis thaliana was provided by Roman Ulm, University of Geneva.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BamHI site
sequence: https://synbiohub.org/public/igem/BBa_K909009_sequence/1

BamHI site
type: SequenceAnnotation
persistent identity: annotation2202567
display id: annotation2202567
version: 1
top level: BBa_K909009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909009/annotation2202567/range2202567/1

https://synbiohub.org/public/igem/BBa_K909009/annotation2202567/range2202567/1
type: Range
persistent identity: range2202567
display id: range2202567
version: 1
direction: direction/forward
top level: BBa_K909009
start: 4
end: 9
orientation: inline

BBa_I6066
type: ComponentDefinition
persistent identity: BBa I6066
display id: BBa_I6066
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0051.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_E0032
component: BBa_R0051
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I6066_sequence/1

BBa_B0034
type: Component
persistent identity: component949090
display id: component949090
version: 1
top level: BBa_I6066
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component949102
display id: component949102
version: 1
top level: BBa_I6066
access: public
definition: YFP

BBa_R0051
type: Component
persistent identity: component949082
display id: component949082
version: 1
top level: BBa_I6066
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0010
type: Component
persistent identity: component949110
display id: component949110
version: 1
top level: BBa_I6066
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949120
display id: component949120
version: 1
top level: BBa_I6066
access: public
definition: BBa_B0012

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation949082
display id: annotation949082
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949082/range949082/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I6066/annotation949082/range949082/1
type: Range
persistent identity: range949082
display id: range949082
version: 1
direction: direction/forward
top level: BBa_I6066
start: 1
end: 49
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949110
display id: annotation949110
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949110/range949110/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6066/annotation949110/range949110/1
type: Range
persistent identity: range949110
display id: range949110
version: 1
direction: direction/forward
top level: BBa_I6066
start: 846
end: 925
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949090
display id: annotation949090
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949090/range949090/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6066/annotation949090/range949090/1
type: Range
persistent identity: range949090
display id: range949090
version: 1
direction: direction/forward
top level: BBa_I6066
start: 58
end: 69
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949120
display id: annotation949120
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949120/range949120/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6066/annotation949120/range949120/1
type: Range
persistent identity: range949120
display id: range949120
version: 1
direction: direction/forward
top level: BBa_I6066
start: 934
end: 974
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949102
display id: annotation949102
version: 1
top level: BBa_I6066
location: https://synbiohub.org/public/igem/BBa_I6066/annotation949102/range949102/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6066/annotation949102/range949102/1
type: Range
persistent identity: range949102
display id: range949102
version: 1
direction: direction/forward
top level: BBa_I6066
start: 76
end: 837
orientation: inline

BBa_I6040
type: ComponentDefinition
persistent identity: BBa I6040
display id: BBa_I6040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0053.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_R0053
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_R0053
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6040_sequence/1

BBa_B0010
type: Component
persistent identity: component948514
display id: component948514
version: 1
top level: BBa_I6040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component948507
display id: component948507
version: 1
top level: BBa_I6040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0053
type: Component
persistent identity: component948497
display id: component948497
version: 1
top level: BBa_I6040
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0012
type: Component
persistent identity: component948524
display id: component948524
version: 1
top level: BBa_I6040
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component948509
display id: component948509
version: 1
top level: BBa_I6040
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948524
display id: annotation948524
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948524/range948524/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6040/annotation948524/range948524/1
type: Range
persistent identity: range948524
display id: range948524
version: 1
direction: direction/forward
top level: BBa_I6040
start: 900
end: 940
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948509
display id: annotation948509
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948509/range948509/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6040/annotation948509/range948509/1
type: Range
persistent identity: range948509
display id: range948509
version: 1
direction: direction/forward
top level: BBa_I6040
start: 81
end: 803
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation948497
display id: annotation948497
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948497/range948497/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I6040/annotation948497/range948497/1
type: Range
persistent identity: range948497
display id: range948497
version: 1
direction: direction/forward
top level: BBa_I6040
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948507
display id: annotation948507
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948507/range948507/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6040/annotation948507/range948507/1
type: Range
persistent identity: range948507
display id: range948507
version: 1
direction: direction/forward
top level: BBa_I6040
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948514
display id: annotation948514
version: 1
top level: BBa_I6040
location: https://synbiohub.org/public/igem/BBa_I6040/annotation948514/range948514/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6040/annotation948514/range948514/1
type: Range
persistent identity: range948514
display id: range948514
version: 1
direction: direction/forward
top level: BBa_I6040
start: 812
end: 891
orientation: inline

HMG-CoA-R
type: ComponentDefinition
persistent identity: BBa K849000
display id: BBa_K849000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast
creator: Leonard Sebastian Fresenborg
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1109_
m user id: 0
owner id: 12733
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a truncated version of the 3-Hydroxy-3-Methyl-Glutaryl-Coenzyme-A-Reductase (HMG-CoA-R) from ''Saccharomyces cerevisiae''. It catalyses the reaction of 3-Hydroxy-3-Methyl-Glutaryle-Coenzyme-A to Mevalonate which occurs in the Mevalonate Pathway to Isopentylpyrophosphate which is the Precurser of all Isoprenoides. The enzyme is naturally a membrane bound enzyme. The membrane anchor is situated N-terminal. This region is also essential for the degradational control of the enzyme. By deleting the N-terminal part to Aminoacid (inclusive) the enzyme is expected to be water soluble and deregulated.
mutable notes: The truncation was done according to the following publication: Rico, Pardo, Orejas: 'Enhanced Production of a Plant Monoterpene by Overexpression of the 3-Hydroxy-3-Methylglutaryl Conenzyme A Reductase Catalytic Domain in Saccharomyces cerevisiae'; Applied and Enviromental Microbiology; Vol 76 p. 6449-6454; 2010
mutable provenance: The original source of this part is the genome of Saccharomyces cerevisiae. We obtained the sequence by de novo synthesis. For that we used data from the Saccharomyes Genome Database. We inserted some calm mutations to avoid conflicts with the Cloning Standard 10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202427
display id: annotation2202427
version: 1
top level: HMG-CoA-R
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849000/annotation2202427/range2202427/1

https://synbiohub.org/public/igem/BBa_K849000/annotation2202427/range2202427/1
type: Range
persistent identity: range2202427
display id: range2202427
version: 1
direction: direction/forward
top level: HMG-CoA-R
start: 1
end: 1584
orientation: inline

BBa_K863204
type: ComponentDefinition
persistent identity: BBa K863204
display id: BBa_K863204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: shuttle vector pECPP11JS for recombination of genes of interest in yeast
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: long description is coming soon
mutable notes: the design considerations are coming soon
mutable provenance: This part is made of the BioBricks XYZ by gibson assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: 3' AOX
sequence annotation: 5' AOX
sequence annotation: Kosak sequence
sequence annotation: AOX1 terminator
sequence annotation: MFalpha
sequence annotation: his4
sequence: https://synbiohub.org/public/igem/BBa_K863204_sequence/1

3' AOX
type: SequenceAnnotation
persistent identity: annotation2200007
display id: annotation2200007
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200007/range2200007/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200007/range2200007/1
type: Range
persistent identity: range2200007
display id: range2200007
version: 1
direction: direction/forward
top level: BBa_K863204
start: 4184
end: 4867
orientation: inline

5' AOX
type: SequenceAnnotation
persistent identity: annotation2200002
display id: annotation2200002
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200002/range2200002/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200002/range2200002/1
type: Range
persistent identity: range2200002
display id: range2200002
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1
end: 940
orientation: inline

Kosak sequence
type: SequenceAnnotation
persistent identity: annotation2200003
display id: annotation2200003
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200003/range2200003/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200003/range2200003/1
type: Range
persistent identity: range2200003
display id: range2200003
version: 1
direction: direction/forward
top level: BBa_K863204
start: 941
end: 952
orientation: inline

AOX1 terminator
type: SequenceAnnotation
persistent identity: annotation2200005
display id: annotation2200005
version: 1
top level: BBa_K863204
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200005/range2200005/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200005/range2200005/1
type: Range
persistent identity: range2200005
display id: range2200005
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1250
end: 1531
orientation: inline

MFalpha
type: SequenceAnnotation
persistent identity: annotation2200004
display id: annotation2200004
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200004/range2200004/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200004/range2200004/1
type: Range
persistent identity: range2200004
display id: range2200004
version: 1
direction: direction/forward
top level: BBa_K863204
start: 953
end: 1215
orientation: inline

his4
type: SequenceAnnotation
persistent identity: annotation2200006
display id: annotation2200006
version: 1
top level: BBa_K863204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200006/range2200006/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200006/range2200006/1
type: Range
persistent identity: range2200006
display id: range2200006
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1615
end: 4143
orientation: inline

BBa_I13523
type: ComponentDefinition
persistent identity: BBa I13523
display id: BBa_I13523
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid v2
creator: jkm
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate in screening plasmid v2 construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0040
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_I13455
sequence annotation: BBa_I13455
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13523_sequence/1

BBa_E0040
type: Component
persistent identity: component1597480
display id: component1597480
version: 1
top level: BBa_I13523
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1597495
display id: component1597495
version: 1
top level: BBa_I13523
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1597478
display id: component1597478
version: 1
top level: BBa_I13523
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1597485
display id: component1597485
version: 1
top level: BBa_I13523
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13455
type: Component
persistent identity: component1597473
display id: component1597473
version: 1
top level: BBa_I13523
access: public
definition: https://synbiohub.org/public/igem/BBa_I13455/1

BBa_I13455
type: SequenceAnnotation
persistent identity: annotation1597473
display id: annotation1597473
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597473/range1597473/1
component: BBa_I13455

https://synbiohub.org/public/igem/BBa_I13523/annotation1597473/range1597473/1
type: Range
persistent identity: range1597473
display id: range1597473
version: 1
direction: direction/forward
top level: BBa_I13523
start: 1
end: 78
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1597478
display id: annotation1597478
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597478/range1597478/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13523/annotation1597478/range1597478/1
type: Range
persistent identity: range1597478
display id: range1597478
version: 1
direction: direction/forward
top level: BBa_I13523
start: 87
end: 98
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1597485
display id: annotation1597485
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597485/range1597485/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13523/annotation1597485/range1597485/1
type: Range
persistent identity: range1597485
display id: range1597485
version: 1
direction: direction/forward
top level: BBa_I13523
start: 833
end: 912
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1597480
display id: annotation1597480
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597480/range1597480/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13523/annotation1597480/range1597480/1
type: Range
persistent identity: range1597480
display id: range1597480
version: 1
direction: direction/forward
top level: BBa_I13523
start: 105
end: 824
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1597495
display id: annotation1597495
version: 1
top level: BBa_I13523
location: https://synbiohub.org/public/igem/BBa_I13523/annotation1597495/range1597495/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13523/annotation1597495/range1597495/1
type: Range
persistent identity: range1597495
display id: range1597495
version: 1
direction: direction/forward
top level: BBa_I13523
start: 921
end: 961
orientation: inline

BBa_J06602
type: ComponentDefinition
persistent identity: BBa J06602
display id: BBa_J06602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Construction Intermediate: mCherry, bacterial with RBS (B0034.J06504)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: true
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Combines BBa_B0034 RBS (Elowitz 1999) with BBa_J06504 mCherry, bacterial. This is an intermediate leading to BBa_J06702.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J06504
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J06504
sequence: https://synbiohub.org/public/igem/BBa_J06602_sequence/1

BBa_J06504
type: Component
persistent identity: component1596334
display id: component1596334
version: 1
top level: BBa_J06602
access: public
definition: mCherry

BBa_B0034
type: Component
persistent identity: component1596326
display id: component1596326
version: 1
top level: BBa_J06602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1596326
display id: annotation1596326
version: 1
top level: BBa_J06602
location: https://synbiohub.org/public/igem/BBa_J06602/annotation1596326/range1596326/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06602/annotation1596326/range1596326/1
type: Range
persistent identity: range1596326
display id: range1596326
version: 1
direction: direction/forward
top level: BBa_J06602
start: 1
end: 12
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1596334
display id: annotation1596334
version: 1
top level: BBa_J06602
location: https://synbiohub.org/public/igem/BBa_J06602/annotation1596334/range1596334/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_J06602/annotation1596334/range1596334/1
type: Range
persistent identity: range1596334
display id: range1596334
version: 1
direction: direction/forward
top level: BBa_J06602
start: 19
end: 732
orientation: inline

BBa_I6102
type: ComponentDefinition
persistent identity: BBa I6102
display id: BBa_I6102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0073.E0430
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0073
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0073
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6102_sequence/1

BBa_R0073
type: Component
persistent identity: component949747
display id: component949747
version: 1
top level: BBa_I6102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0073/1

BBa_B0010
type: Component
persistent identity: component949759
display id: component949759
version: 1
top level: BBa_I6102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949769
display id: component949769
version: 1
top level: BBa_I6102
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component949754
display id: component949754
version: 1
top level: BBa_I6102
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component949752
display id: component949752
version: 1
top level: BBa_I6102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949769
display id: annotation949769
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949769/range949769/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6102/annotation949769/range949769/1
type: Range
persistent identity: range949769
display id: range949769
version: 1
direction: direction/forward
top level: BBa_I6102
start: 913
end: 953
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949752
display id: annotation949752
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949752/range949752/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6102/annotation949752/range949752/1
type: Range
persistent identity: range949752
display id: range949752
version: 1
direction: direction/forward
top level: BBa_I6102
start: 76
end: 87
orientation: inline

BBa_R0073
type: SequenceAnnotation
persistent identity: annotation949747
display id: annotation949747
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949747/range949747/1
component: BBa_R0073

https://synbiohub.org/public/igem/BBa_I6102/annotation949747/range949747/1
type: Range
persistent identity: range949747
display id: range949747
version: 1
direction: direction/forward
top level: BBa_I6102
start: 1
end: 67
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949754
display id: annotation949754
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949754/range949754/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6102/annotation949754/range949754/1
type: Range
persistent identity: range949754
display id: range949754
version: 1
direction: direction/forward
top level: BBa_I6102
start: 94
end: 816
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949759
display id: annotation949759
version: 1
top level: BBa_I6102
location: https://synbiohub.org/public/igem/BBa_I6102/annotation949759/range949759/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6102/annotation949759/range949759/1
type: Range
persistent identity: range949759
display id: range949759
version: 1
direction: direction/forward
top level: BBa_I6102
start: 825
end: 904
orientation: inline

BBa_I732810
type: ComponentDefinition
persistent identity: BBa I732810
display id: BBa_I732810
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0203 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732110
component: BBa_B0034
sequence annotation: BBa_I732110
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732810_sequence/1

BBa_I732110
type: Component
persistent identity: component1946193
display id: component1946193
version: 1
top level: BBa_I732810
access: public
definition: https://synbiohub.org/public/igem/BBa_I732110/1

BBa_B0034
type: Component
persistent identity: component1946192
display id: component1946192
version: 1
top level: BBa_I732810
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732110
type: SequenceAnnotation
persistent identity: annotation1946193
display id: annotation1946193
version: 1
top level: BBa_I732810
location: https://synbiohub.org/public/igem/BBa_I732810/annotation1946193/range1946193/1
component: BBa_I732110

https://synbiohub.org/public/igem/BBa_I732810/annotation1946193/range1946193/1
type: Range
persistent identity: range1946193
display id: range1946193
version: 1
direction: direction/reverse
top level: BBa_I732810
start: 19
end: 1104
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946192
display id: annotation1946192
version: 1
top level: BBa_I732810
location: https://synbiohub.org/public/igem/BBa_I732810/annotation1946192/range1946192/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732810/annotation1946192/range1946192/1
type: Range
persistent identity: range1946192
display id: range1946192
version: 1
direction: direction/reverse
top level: BBa_I732810
start: 1
end: 12
orientation: inline

BBa_K876063
type: ComponentDefinition
persistent identity: BBa K876063
display id: BBa_K876063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI-RBS-YFP-Term-Term
creator: Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: intermediate part
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0061
component: BBa_E0432
sequence annotation: BBa_E0432
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_K876063_sequence/1

BBa_C0061
type: Component
persistent identity: component2259770
display id: component2259770
version: 1
top level: BBa_K876063
access: public
definition: luxI

BBa_E0432
type: Component
persistent identity: component2259786
display id: component2259786
version: 1
top level: BBa_K876063
access: public
definition: https://synbiohub.org/public/igem/BBa_E0432/1

BBa_E0432
type: SequenceAnnotation
persistent identity: annotation2259786
display id: annotation2259786
version: 1
top level: BBa_K876063
location: https://synbiohub.org/public/igem/BBa_K876063/annotation2259786/range2259786/1
component: BBa_E0432

https://synbiohub.org/public/igem/BBa_K876063/annotation2259786/range2259786/1
type: Range
persistent identity: range2259786
display id: range2259786
version: 1
direction: direction/reverse
top level: BBa_K876063
start: 652
end: 1568
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2259770
display id: annotation2259770
version: 1
top level: BBa_K876063
location: https://synbiohub.org/public/igem/BBa_K876063/annotation2259770/range2259770/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876063/annotation2259770/range2259770/1
type: Range
persistent identity: range2259770
display id: range2259770
version: 1
direction: direction/forward
top level: BBa_K876063
start: 1
end: 618
orientation: inline

BBa_K747066
type: ComponentDefinition
persistent identity: BBa K747066
display id: BBa_K747066
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 5
sequence: https://synbiohub.org/public/igem/BBa_K747066_sequence/1

AG 5
type: SequenceAnnotation
persistent identity: annotation2197023
display id: annotation2197023
version: 1
top level: BBa_K747066
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747066/annotation2197023/range2197023/1

https://synbiohub.org/public/igem/BBa_K747066/annotation2197023/range2197023/1
type: Range
persistent identity: range2197023
display id: range2197023
version: 1
direction: direction/forward
top level: BBa_K747066
start: 14
end: 211
orientation: inline

BBa_I13542
type: ComponentDefinition
persistent identity: BBa I13542
display id: BBa_I13542
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate in two promoter screening plasmid
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13453
component: BBa_B0034
component: BBa_B0010
component: BBa_I13457
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_I13453
sequence annotation: BBa_I13457
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13542_sequence/1

BBa_I13453
type: Component
persistent identity: component1663901
display id: component1663901
version: 1
top level: BBa_I13542
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: Component
persistent identity: component1663926
display id: component1663926
version: 1
top level: BBa_I13542
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1663939
display id: component1663939
version: 1
top level: BBa_I13542
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13457
type: Component
persistent identity: component1663921
display id: component1663921
version: 1
top level: BBa_I13542
access: public
definition: BBa_I13457

BBa_B0012
type: Component
persistent identity: component1663949
display id: component1663949
version: 1
top level: BBa_I13542
access: public
definition: BBa_B0012

BBa_E1010
type: Component
persistent identity: component1663933
display id: component1663933
version: 1
top level: BBa_I13542
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663901
display id: annotation1663901
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663901/range1663901/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13542/annotation1663901/range1663901/1
type: Range
persistent identity: range1663901
display id: range1663901
version: 1
direction: direction/forward
top level: BBa_I13542
start: 1
end: 130
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1663921
display id: annotation1663921
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663921/range1663921/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13542/annotation1663921/range1663921/1
type: Range
persistent identity: range1663921
display id: range1663921
version: 1
direction: direction/forward
top level: BBa_I13542
start: 139
end: 249
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1663933
display id: annotation1663933
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663933/range1663933/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13542/annotation1663933/range1663933/1
type: Range
persistent identity: range1663933
display id: range1663933
version: 1
direction: direction/forward
top level: BBa_I13542
start: 276
end: 956
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663939
display id: annotation1663939
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663939/range1663939/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13542/annotation1663939/range1663939/1
type: Range
persistent identity: range1663939
display id: range1663939
version: 1
direction: direction/forward
top level: BBa_I13542
start: 990
end: 1069
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663926
display id: annotation1663926
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663926/range1663926/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13542/annotation1663926/range1663926/1
type: Range
persistent identity: range1663926
display id: range1663926
version: 1
direction: direction/forward
top level: BBa_I13542
start: 258
end: 269
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663949
display id: annotation1663949
version: 1
top level: BBa_I13542
location: https://synbiohub.org/public/igem/BBa_I13542/annotation1663949/range1663949/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13542/annotation1663949/range1663949/1
type: Range
persistent identity: range1663949
display id: range1663949
version: 1
direction: direction/forward
top level: BBa_I13542
start: 1078
end: 1118
orientation: inline

BBa_K929002
type: ComponentDefinition
persistent identity: BBa K929002
display id: BBa_K929002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified AID with CMV and hGH-polyA
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14457
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K929001). It is built of 3 parts: CMV promoter (BBa_I712004), superAID and hGH polyadenylation signal sequence (BBa_K404108). AID: AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes. The AID motif is naturally terminated with the Nuclear Export Sequence (NES) that causes the protein to translocate from the nucleus to the cytoplasm. Additionally, upstream, dysfunctional Nuclear Localization Sequence (NLS) is located. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate. That is why we called it superAID Functional NLS sequence: This part of the BioBrick directs the expressed protein into the nucleus, where it can mutate stronger. Kozak sequence Kozak consensus sequence is added upstream of the AID mutant to express the protein stronger. CMV promoter: CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter. hGH polyadenylation signal sequence: Polyadenylation is a significant part for the translation and stability of mRNA. In eukaryotes, it is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression. hGH terminator gives a signal to start polyadenylation in the translation proce…
mutable notes: blaa bla
mutable provenance: 3 other Biobricks: CMV promoter (BBa_I712004), superAID (BBa_K929001) and hGH polyadenylation signal sequence (BBa_K404108).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K929001
sequence annotation: AgeI
sequence annotation: NLS sequence
sequence annotation: mod. AID
sequence annotation: Kozak sequence
sequence annotation: AUG
sequence annotation: hGH terminator
sequence annotation: CMV promoter
sequence annotation: BBa_I712004
sequence annotation: UAA
sequence annotation: BBa_K404108
sequence: https://synbiohub.org/public/igem/BBa_K929002_sequence/1

BBa_K929001
type: SequenceAnnotation
persistent identity: annotation2184473
display id: annotation2184473
version: 1
top level: BBa_K929002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184473/range2184473/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184473/range2184473/1
type: Range
persistent identity: range2184473
display id: range2184473
version: 1
direction: direction/forward
top level: BBa_K929002
start: 663
end: 1249
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2196257
display id: annotation2196257
version: 1
top level: BBa_K929002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2196257/range2196257/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2196257/range2196257/1
type: Range
persistent identity: range2196257
display id: range2196257
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1241
end: 1246
orientation: inline

NLS sequence
type: SequenceAnnotation
persistent identity: annotation2184471
display id: annotation2184471
version: 1
top level: BBa_K929002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184471/range2184471/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184471/range2184471/1
type: Range
persistent identity: range2184471
display id: range2184471
version: 1
direction: direction/forward
top level: BBa_K929002
start: 678
end: 695
orientation: inline

mod. AID
type: SequenceAnnotation
persistent identity: annotation2184472
display id: annotation2184472
version: 1
top level: BBa_K929002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184472/range2184472/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184472/range2184472/1
type: Range
persistent identity: range2184472
display id: range2184472
version: 1
direction: direction/forward
top level: BBa_K929002
start: 696
end: 1250
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2184470
display id: annotation2184470
version: 1
top level: BBa_K929002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184470/range2184470/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184470/range2184470/1
type: Range
persistent identity: range2184470
display id: range2184470
version: 1
direction: direction/forward
top level: BBa_K929002
start: 663
end: 677
orientation: inline

AUG
type: SequenceAnnotation
persistent identity: annotation2193793
display id: annotation2193793
version: 1
top level: BBa_K929002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2193793/range2193793/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2193793/range2193793/1
type: Range
persistent identity: range2193793
display id: range2193793
version: 1
direction: direction/forward
top level: BBa_K929002
start: 671
end: 673
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2184474
display id: annotation2184474
version: 1
top level: BBa_K929002
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184474/range2184474/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184474/range2184474/1
type: Range
persistent identity: range2184474
display id: range2184474
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1259
end: 1736
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2184468
display id: annotation2184468
version: 1
top level: BBa_K929002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184468/range2184468/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184468/range2184468/1
type: Range
persistent identity: range2184468
display id: range2184468
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1
end: 654
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2184469
display id: annotation2184469
version: 1
top level: BBa_K929002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184469/range2184469/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184469/range2184469/1
type: Range
persistent identity: range2184469
display id: range2184469
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1
end: 654
orientation: inline

UAA
type: SequenceAnnotation
persistent identity: annotation2193794
display id: annotation2193794
version: 1
top level: BBa_K929002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2193794/range2193794/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2193794/range2193794/1
type: Range
persistent identity: range2193794
display id: range2193794
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1247
end: 1249
orientation: inline

BBa_K404108
type: SequenceAnnotation
persistent identity: annotation2184475
display id: annotation2184475
version: 1
top level: BBa_K929002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929002/annotation2184475/range2184475/1

https://synbiohub.org/public/igem/BBa_K929002/annotation2184475/range2184475/1
type: Range
persistent identity: range2184475
display id: range2184475
version: 1
direction: direction/forward
top level: BBa_K929002
start: 1259
end: 1736
orientation: inline

BBa_P0440
type: ComponentDefinition
persistent identity: BBa P0440
display id: BBa_P0440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0151]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0440_sequence/1

BBa_B0034
type: Component
persistent identity: component944886
display id: component944886
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component944912
display id: component944912
version: 1
top level: BBa_P0440
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component944902
display id: component944902
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0040
type: Component
persistent identity: component944896
display id: component944896
version: 1
top level: BBa_P0440
access: public
definition: tetR

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944902
display id: annotation944902
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
type: Range
persistent identity: range944902
display id: range944902
version: 1
direction: direction/forward
top level: BBa_P0440
start: 712
end: 791
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944886
display id: annotation944886
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
type: Range
persistent identity: range944886
display id: range944886
version: 1
direction: direction/forward
top level: BBa_P0440
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944896
display id: annotation944896
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
type: Range
persistent identity: range944896
display id: range944896
version: 1
direction: direction/forward
top level: BBa_P0440
start: 19
end: 678
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944912
display id: annotation944912
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
type: Range
persistent identity: range944912
display id: range944912
version: 1
direction: direction/forward
top level: BBa_P0440
start: 800
end: 840
orientation: inline

BBa_K574005
type: ComponentDefinition
persistent identity: BBa K574005
display id: BBa_K574005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL and YFP regulated by pBad
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pBAD + lasI + yfp.
mutable notes: Empty.
mutable provenance: Exsiting Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_E0430
component: BBa_I13453
component: BBa_K081009
sequence annotation: BBa_E0430
sequence annotation: BBa_K081009
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K574005_sequence/1

BBa_E0430
type: Component
persistent identity: component2268889
display id: component2268889
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_I13453
type: Component
persistent identity: component2268871
display id: component2268871
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K081009
type: Component
persistent identity: component2268879
display id: component2268879
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_K081009/1

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2268889
display id: annotation2268889
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268889/range2268889/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_K574005/annotation2268889/range2268889/1
type: Range
persistent identity: range2268889
display id: range2268889
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 835
end: 1712
orientation: inline

BBa_K081009
type: SequenceAnnotation
persistent identity: annotation2268879
display id: annotation2268879
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268879/range2268879/1
component: BBa_K081009

https://synbiohub.org/public/igem/BBa_K574005/annotation2268879/range2268879/1
type: Range
persistent identity: range2268879
display id: range2268879
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 139
end: 826
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2268871
display id: annotation2268871
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268871/range2268871/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K574005/annotation2268871/range2268871/1
type: Range
persistent identity: range2268871
display id: range2268871
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 1
end: 130
orientation: inline

BBa_K747083
type: ComponentDefinition
persistent identity: BBa K747083
display id: BBa_K747083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 6
sequence: https://synbiohub.org/public/igem/BBa_K747083_sequence/1

AT 6
type: SequenceAnnotation
persistent identity: annotation2197056
display id: annotation2197056
version: 1
top level: BBa_K747083
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747083/annotation2197056/range2197056/1

https://synbiohub.org/public/igem/BBa_K747083/annotation2197056/range2197056/1
type: Range
persistent identity: range2197056
display id: range2197056
version: 1
direction: direction/forward
top level: BBa_K747083
start: 14
end: 211
orientation: inline

BBa_S01003
type: ComponentDefinition
persistent identity: BBa S01003
display id: BBa_S01003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0062.E0422
creator: Randy Rettberg
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 tube 45
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_E0022
component: BBa_R0062
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S01003_sequence/1

BBa_B0012
type: Component
persistent identity: component1511012
display id: component1511012
version: 1
top level: BBa_S01003
access: public
definition: BBa_B0012

BBa_E0022
type: Component
persistent identity: component1510994
display id: component1510994
version: 1
top level: BBa_S01003
access: public
definition: ECFP

BBa_R0062
type: Component
persistent identity: component1510974
display id: component1510974
version: 1
top level: BBa_S01003
access: public
definition: lux pR

BBa_B0010
type: Component
persistent identity: component1511002
display id: component1511002
version: 1
top level: BBa_S01003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1510982
display id: component1510982
version: 1
top level: BBa_S01003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1510974
display id: annotation1510974
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1510974/range1510974/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_S01003/annotation1510974/range1510974/1
type: Range
persistent identity: range1510974
display id: range1510974
version: 1
direction: direction/forward
top level: BBa_S01003
start: 1
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511012
display id: annotation1511012
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1511012/range1511012/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01003/annotation1511012/range1511012/1
type: Range
persistent identity: range1511012
display id: range1511012
version: 1
direction: direction/forward
top level: BBa_S01003
start: 940
end: 980
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1510994
display id: annotation1510994
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1510994/range1510994/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_S01003/annotation1510994/range1510994/1
type: Range
persistent identity: range1510994
display id: range1510994
version: 1
direction: direction/forward
top level: BBa_S01003
start: 82
end: 843
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1510982
display id: annotation1510982
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1510982/range1510982/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01003/annotation1510982/range1510982/1
type: Range
persistent identity: range1510982
display id: range1510982
version: 1
direction: direction/forward
top level: BBa_S01003
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511002
display id: annotation1511002
version: 1
top level: BBa_S01003
location: https://synbiohub.org/public/igem/BBa_S01003/annotation1511002/range1511002/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01003/annotation1511002/range1511002/1
type: Range
persistent identity: range1511002
display id: range1511002
version: 1
direction: direction/forward
top level: BBa_S01003
start: 852
end: 931
orientation: inline

[pTET][loc
type: ComponentDefinition
persistent identity: BBa J01060
display id: BBa_J01060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OnLock1 = [pTet][Lock1]
creator: Golden Bear
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [pTet][Lock1]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J01010
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J01010
sequence: https://synbiohub.org/public/igem/BBa_J01060_sequence/1

BBa_J01010
type: Component
persistent identity: component1738596
display id: component1738596
version: 1
top level: [pTET][loc
access: public
definition: https://synbiohub.org/public/igem/BBa_J01010/1

BBa_R0040
type: Component
persistent identity: component1738591
display id: component1738591
version: 1
top level: [pTET][loc
access: public
definition: p(tetR)

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1738591
display id: annotation1738591
version: 1
top level: [pTET][loc
location: https://synbiohub.org/public/igem/BBa_J01060/annotation1738591/range1738591/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J01060/annotation1738591/range1738591/1
type: Range
persistent identity: range1738591
display id: range1738591
version: 1
direction: direction/forward
top level: [pTET][loc
start: 1
end: 54
orientation: inline

BBa_J01010
type: SequenceAnnotation
persistent identity: annotation1738596
display id: annotation1738596
version: 1
top level: [pTET][loc
location: https://synbiohub.org/public/igem/BBa_J01060/annotation1738596/range1738596/1
component: BBa_J01010

https://synbiohub.org/public/igem/BBa_J01060/annotation1738596/range1738596/1
type: Range
persistent identity: range1738596
display id: range1738596
version: 1
direction: direction/forward
top level: [pTET][loc
start: 63
end: 102
orientation: inline

BBa_K747061
type: ComponentDefinition
persistent identity: BBa K747061
display id: BBa_K747061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 4
sequence: https://synbiohub.org/public/igem/BBa_K747061_sequence/1

TC 4
type: SequenceAnnotation
persistent identity: annotation2197018
display id: annotation2197018
version: 1
top level: BBa_K747061
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747061/annotation2197018/range2197018/1

https://synbiohub.org/public/igem/BBa_K747061/annotation2197018/range2197018/1
type: Range
persistent identity: range2197018
display id: range2197018
version: 1
direction: direction/forward
top level: BBa_K747061
start: 14
end: 211
orientation: inline

BBa_K747070
type: ComponentDefinition
persistent identity: BBa K747070
display id: BBa_K747070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 5
sequence: https://synbiohub.org/public/igem/BBa_K747070_sequence/1

CG 5
type: SequenceAnnotation
persistent identity: annotation2197035
display id: annotation2197035
version: 1
top level: BBa_K747070
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747070/annotation2197035/range2197035/1

https://synbiohub.org/public/igem/BBa_K747070/annotation2197035/range2197035/1
type: Range
persistent identity: range2197035
display id: range2197035
version: 1
direction: direction/forward
top level: BBa_K747070
start: 14
end: 211
orientation: inline

BBa_K747013
type: ComponentDefinition
persistent identity: BBa K747013
display id: BBa_K747013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 1
sequence: https://synbiohub.org/public/igem/BBa_K747013_sequence/1

TC 1
type: SequenceAnnotation
persistent identity: annotation2195923
display id: annotation2195923
version: 1
top level: BBa_K747013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747013/annotation2195923/range2195923/1

https://synbiohub.org/public/igem/BBa_K747013/annotation2195923/range2195923/1
type: Range
persistent identity: range2195923
display id: range2195923
version: 1
direction: direction/forward
top level: BBa_K747013
start: 12
end: 209
orientation: inline

BBa_K801071
type: ComponentDefinition
persistent identity: BBa K801071
display id: BBa_K801071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7-methylxanthine N-methyltransferase CaMXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 7-methylxanthine N-methyltransferase CaMXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: Synthesized by IDT, Yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Consensus sequence
sequence annotation: CaMXMT1
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801071_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2198586
display id: annotation2198586
version: 1
top level: BBa_K801071
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1
type: Range
persistent identity: range2198586
display id: range2198586
version: 1
direction: direction/forward
top level: BBa_K801071
start: 7
end: 9
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197876
display id: annotation2197876
version: 1
top level: BBa_K801071
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1
type: Range
persistent identity: range2197876
display id: range2197876
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1
end: 6
orientation: inline

CaMXMT1
type: SequenceAnnotation
persistent identity: annotation2197709
display id: annotation2197709
version: 1
top level: BBa_K801071
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1
type: Range
persistent identity: range2197709
display id: range2197709
version: 1
direction: direction/forward
top level: BBa_K801071
start: 7
end: 1140
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198148
display id: annotation2198148
version: 1
top level: BBa_K801071
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1
type: Range
persistent identity: range2198148
display id: range2198148
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1141
end: 1170
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198587
display id: annotation2198587
version: 1
top level: BBa_K801071
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1
type: Range
persistent identity: range2198587
display id: range2198587
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1171
end: 1176
orientation: inline

BBa_K737007
type: ComponentDefinition
persistent identity: BBa K737007
display id: BBa_K737007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive GVPC Generator
creator: Li Kang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: "It contains a constitutive promoter, GvpC protein , GFP protein and a terminator. This part can be used as a control of (J23106+P0440+R0040+B0034+GVPC+E0840). It can express GvpC protein and GFP constantly. When it transformed with (J23106+P0412+R0011+B0034+GVPA+E0840), the bacteria may can float with GFP!"
mutable notes: This part can be used as a control of (J23106+P0440+R0040+B0034+GVPC+E0840). It can express GvpC protein and GFP constantly. When it transformed with (J23106+P0412+R0011+B0034+GVPA+E0840), the bacteria may can float with GFP.
mutable provenance: Yes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_E0840
component: BBa_J23106
component: BBa_K737017
sequence annotation: BBa_J23106
sequence annotation: BBa_E0840
sequence annotation: BBa_B0034
sequence annotation: BBa_K737017
sequence: https://synbiohub.org/public/igem/BBa_K737007_sequence/1

BBa_B0034
type: Component
persistent identity: component2184892
display id: component2184892
version: 1
top level: BBa_K737007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0840
type: Component
persistent identity: component2184904
display id: component2184904
version: 1
top level: BBa_K737007
access: public
definition: GFP genera

BBa_J23106
type: Component
persistent identity: component2184890
display id: component2184890
version: 1
top level: BBa_K737007
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_K737017
type: Component
persistent identity: component2184893
display id: component2184893
version: 1
top level: BBa_K737007
access: public
definition: https://synbiohub.org/public/igem/BBa_K737017/1

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2184890
display id: annotation2184890
version: 1
top level: BBa_K737007
location: https://synbiohub.org/public/igem/BBa_K737007/annotation2184890/range2184890/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K737007/annotation2184890/range2184890/1
type: Range
persistent identity: range2184890
display id: range2184890
version: 1
direction: direction/reverse
top level: BBa_K737007
start: 1
end: 35
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2184904
display id: annotation2184904
version: 1
top level: BBa_K737007
location: https://synbiohub.org/public/igem/BBa_K737007/annotation2184904/range2184904/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K737007/annotation2184904/range2184904/1
type: Range
persistent identity: range2184904
display id: range2184904
version: 1
direction: direction/reverse
top level: BBa_K737007
start: 574
end: 1451
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2184892
display id: annotation2184892
version: 1
top level: BBa_K737007
location: https://synbiohub.org/public/igem/BBa_K737007/annotation2184892/range2184892/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K737007/annotation2184892/range2184892/1
type: Range
persistent identity: range2184892
display id: range2184892
version: 1
direction: direction/reverse
top level: BBa_K737007
start: 44
end: 55
orientation: inline

BBa_K737017
type: SequenceAnnotation
persistent identity: annotation2184893
display id: annotation2184893
version: 1
top level: BBa_K737007
location: https://synbiohub.org/public/igem/BBa_K737007/annotation2184893/range2184893/1
component: BBa_K737017

https://synbiohub.org/public/igem/BBa_K737007/annotation2184893/range2184893/1
type: Range
persistent identity: range2184893
display id: range2184893
version: 1
direction: direction/reverse
top level: BBa_K737007
start: 62
end: 565
orientation: inline

BBa_K747049
type: ComponentDefinition
persistent identity: BBa K747049
display id: BBa_K747049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 4
sequence: https://synbiohub.org/public/igem/BBa_K747049_sequence/1

AC 4
type: SequenceAnnotation
persistent identity: annotation2196276
display id: annotation2196276
version: 1
top level: BBa_K747049
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747049/annotation2196276/range2196276/1

https://synbiohub.org/public/igem/BBa_K747049/annotation2196276/range2196276/1
type: Range
persistent identity: range2196276
display id: range2196276
version: 1
direction: direction/forward
top level: BBa_K747049
start: 14
end: 211
orientation: inline

Bxb1_Int
type: ComponentDefinition
persistent identity: BBa K907000
display id: BBa_K907000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mycobacterium Phage Bxb1 gp35, DNA integrase
creator: Dong-hui Choe, Soo-in Lee
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1172_
m user id: 0
owner id: 12628
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1. The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences. We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655). When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.
mutable notes: Internal restriction endonuclease site is changed(two PstI sites, one XbaI sites) without changing amino acid sequence.
mutable provenance: This part is derived by Mycobacterium phage Bxb1. And synthesized by Bioneer. http://www.ncbi.nlm.nih.gov/protein/NP_075302.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mycobacterium phage Bxb1 integrase CDS
sequence: https://synbiohub.org/public/igem/BBa_K907000_sequence/1

Mycobacterium phage Bxb1 integrase CDS
type: SequenceAnnotation
persistent identity: annotation2187533
display id: annotation2187533
version: 1
top level: Bxb1_Int
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K907000/annotation2187533/range2187533/1

https://synbiohub.org/public/igem/BBa_K907000/annotation2187533/range2187533/1
type: Range
persistent identity: range2187533
display id: range2187533
version: 1
direction: direction/forward
top level: Bxb1_Int
start: 1
end: 1503
orientation: inline

BBa_K747033
type: ComponentDefinition
persistent identity: BBa K747033
display id: BBa_K747033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 3
sequence: https://synbiohub.org/public/igem/BBa_K747033_sequence/1

AT 3
type: SequenceAnnotation
persistent identity: annotation2196208
display id: annotation2196208
version: 1
top level: BBa_K747033
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747033/annotation2196208/range2196208/1

https://synbiohub.org/public/igem/BBa_K747033/annotation2196208/range2196208/1
type: Range
persistent identity: range2196208
display id: range2196208
version: 1
direction: direction/forward
top level: BBa_K747033
start: 14
end: 211
orientation: inline

BBa_K747077
type: ComponentDefinition
persistent identity: BBa K747077
display id: BBa_K747077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 5
sequence: https://synbiohub.org/public/igem/BBa_K747077_sequence/1

TC 5
type: SequenceAnnotation
persistent identity: annotation2197046
display id: annotation2197046
version: 1
top level: BBa_K747077
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747077/annotation2197046/range2197046/1

https://synbiohub.org/public/igem/BBa_K747077/annotation2197046/range2197046/1
type: Range
persistent identity: range2197046
display id: range2197046
version: 1
direction: direction/forward
top level: BBa_K747077
start: 14
end: 211
orientation: inline

BBa_J202006
type: ComponentDefinition
persistent identity: BBa J202006
display id: BBa_J202006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#7
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -66 relative to the araC transcriptional start site was changed from T to G.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Start
sequence annotation: araI1
sequence annotation: Mutation
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202006_sequence/1

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178440
display id: annotation2178440
version: 1
top level: BBa_J202006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202006/annotation2178440/range2178440/1

https://synbiohub.org/public/igem/BBa_J202006/annotation2178440/range2178440/1
type: Range
persistent identity: range2178440
display id: range2178440
version: 1
direction: direction/forward
top level: BBa_J202006
start: 112
end: 112
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178441
display id: annotation2178441
version: 1
top level: BBa_J202006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202006/annotation2178441/range2178441/1

https://synbiohub.org/public/igem/BBa_J202006/annotation2178441/range2178441/1
type: Range
persistent identity: range2178441
display id: range2178441
version: 1
direction: direction/forward
top level: BBa_J202006
start: 40
end: 56
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178443
display id: annotation2178443
version: 1
top level: BBa_J202006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202006/annotation2178443/range2178443/1

https://synbiohub.org/public/igem/BBa_J202006/annotation2178443/range2178443/1
type: Range
persistent identity: range2178443
display id: range2178443
version: 1
direction: direction/forward
top level: BBa_J202006
start: 46
end: 46
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178442
display id: annotation2178442
version: 1
top level: BBa_J202006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202006/annotation2178442/range2178442/1

https://synbiohub.org/public/igem/BBa_J202006/annotation2178442/range2178442/1
type: Range
persistent identity: range2178442
display id: range2178442
version: 1
direction: direction/forward
top level: BBa_J202006
start: 61
end: 77
orientation: inline

BBa_K775001
type: ComponentDefinition
persistent identity: BBa K775001
display id: BBa_K775001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-Olfr154 Isoamylacetate chimeric GPCR
creator: Sietske Grijseels
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775001 encodes a chimeric isoamylacetate-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mouse Olfr154, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: -
mutable provenance: N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: Olfr154 NM_013728, Mus musculus, (mouse) C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K775001_sequence/1

BBa_K769020
type: ComponentDefinition
persistent identity: BBa K769020
display id: BBa_K769020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lux operon(K769011) + lumP(K769019)
creator: Tomomi YOKOYAMA
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1021_
m user id: 0
owner id: 14294
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter(Pbad)-lux operon-DT + lumP(K769019)
mutable notes: blue shift
mutable provenance: P.kishitanii
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K769011
component: BBa_K769019
sequence annotation: BBa_K769011
sequence annotation: BBa_K769019
sequence: https://synbiohub.org/public/igem/BBa_K769020_sequence/1

BBa_K769011
type: Component
persistent identity: component2186406
display id: component2186406
version: 1
top level: BBa_K769020
access: public
definition: https://synbiohub.org/public/igem/BBa_K769011/1

BBa_K769019
type: Component
persistent identity: component2186418
display id: component2186418
version: 1
top level: BBa_K769020
access: public
definition: https://synbiohub.org/public/igem/BBa_K769019/1

BBa_K769011
type: SequenceAnnotation
persistent identity: annotation2186406
display id: annotation2186406
version: 1
top level: BBa_K769020
location: https://synbiohub.org/public/igem/BBa_K769020/annotation2186406/range2186406/1
component: BBa_K769011

https://synbiohub.org/public/igem/BBa_K769020/annotation2186406/range2186406/1
type: Range
persistent identity: range2186406
display id: range2186406
version: 1
direction: direction/reverse
top level: BBa_K769020
start: 1
end: 8531
orientation: inline

BBa_K769019
type: SequenceAnnotation
persistent identity: annotation2186418
display id: annotation2186418
version: 1
top level: BBa_K769020
location: https://synbiohub.org/public/igem/BBa_K769020/annotation2186418/range2186418/1
component: BBa_K769019

https://synbiohub.org/public/igem/BBa_K769020/annotation2186418/range2186418/1
type: Range
persistent identity: range2186418
display id: range2186418
version: 1
direction: direction/reverse
top level: BBa_K769020
start: 8540
end: 9301
orientation: inline

BBa_K741020
type: ComponentDefinition
persistent identity: BBa K741020
display id: BBa_K741020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T
creator: Wuyang Chen
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: We make this part in the middle of the progress of making the part plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T-pRM-anticro-T.
mutable notes: TBD
mutable provenance: BBa_K741001 BBa_K741009
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K741001
component: BBa_K741009
sequence annotation: BBa_K741001
sequence annotation: BBa_K741009
sequence: https://synbiohub.org/public/igem/BBa_K741020_sequence/1

BBa_K741001
type: Component
persistent identity: component2230895
display id: component2230895
version: 1
top level: BBa_K741020
access: public
definition: https://synbiohub.org/public/igem/BBa_K741001/1

BBa_K741009
type: Component
persistent identity: component2230908
display id: component2230908
version: 1
top level: BBa_K741020
access: public
definition: https://synbiohub.org/public/igem/BBa_K741009/1

BBa_K741001
type: SequenceAnnotation
persistent identity: annotation2230895
display id: annotation2230895
version: 1
top level: BBa_K741020
location: https://synbiohub.org/public/igem/BBa_K741020/annotation2230895/range2230895/1
component: BBa_K741001

https://synbiohub.org/public/igem/BBa_K741020/annotation2230895/range2230895/1
type: Range
persistent identity: range2230895
display id: range2230895
version: 1
direction: direction/reverse
top level: BBa_K741020
start: 1
end: 1138
orientation: inline

BBa_K741009
type: SequenceAnnotation
persistent identity: annotation2230908
display id: annotation2230908
version: 1
top level: BBa_K741020
location: https://synbiohub.org/public/igem/BBa_K741020/annotation2230908/range2230908/1
component: BBa_K741009

https://synbiohub.org/public/igem/BBa_K741020/annotation2230908/range2230908/1
type: Range
persistent identity: range2230908
display id: range2230908
version: 1
direction: direction/reverse
top level: BBa_K741020
start: 1147
end: 2277
orientation: inline

BBa_K747024
type: ComponentDefinition
persistent identity: BBa K747024
display id: BBa_K747024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 2
sequence: https://synbiohub.org/public/igem/BBa_K747024_sequence/1

GA 2
type: SequenceAnnotation
persistent identity: annotation2196062
display id: annotation2196062
version: 1
top level: BBa_K747024
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747024/annotation2196062/range2196062/1

https://synbiohub.org/public/igem/BBa_K747024/annotation2196062/range2196062/1
type: Range
persistent identity: range2196062
display id: range2196062
version: 1
direction: direction/forward
top level: BBa_K747024
start: 14
end: 211
orientation: inline

BBa_K747076
type: ComponentDefinition
persistent identity: BBa K747076
display id: BBa_K747076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This Part was synthesized and flanked with BsmBI-restriction sites.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 5
sequence: https://synbiohub.org/public/igem/BBa_K747076_sequence/1

TA 5
type: SequenceAnnotation
persistent identity: annotation2197045
display id: annotation2197045
version: 1
top level: BBa_K747076
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747076/annotation2197045/range2197045/1

https://synbiohub.org/public/igem/BBa_K747076/annotation2197045/range2197045/1
type: Range
persistent identity: range2197045
display id: range2197045
version: 1
direction: direction/forward
top level: BBa_K747076
start: 14
end: 211
orientation: inline

BBa_S0116
type: ComponentDefinition
persistent identity: BBa S0116
display id: BBa_S0116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0031.C0052
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0052
component: BBa_B0031
sequence annotation: BBa_C0052
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_S0116_sequence/1

BBa_C0052
type: Component
persistent identity: component939984
display id: component939984
version: 1
top level: BBa_S0116
access: public
definition: cI 434

BBa_B0031
type: Component
persistent identity: component939974
display id: component939974
version: 1
top level: BBa_S0116
access: public
definition: BBa_B0031

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation939984
display id: annotation939984
version: 1
top level: BBa_S0116
location: https://synbiohub.org/public/igem/BBa_S0116/annotation939984/range939984/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_S0116/annotation939984/range939984/1
type: Range
persistent identity: range939984
display id: range939984
version: 1
direction: direction/forward
top level: BBa_S0116
start: 21
end: 689
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation939974
display id: annotation939974
version: 1
top level: BBa_S0116
location: https://synbiohub.org/public/igem/BBa_S0116/annotation939974/range939974/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_S0116/annotation939974/range939974/1
type: Range
persistent identity: range939974
display id: range939974
version: 1
direction: direction/forward
top level: BBa_S0116
start: 1
end: 14
orientation: inline

BBa_K808034
type: ComponentDefinition
persistent identity: BBa K808034
display id: BBa_K808034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad regulated terephtalate periplasmatic binding proteine of the tripartite transporter family
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K808000
component: BBa_J61101
component: BBa_K808001
sequence annotation: BBa_J61101
sequence annotation: BBa_K808001
sequence annotation: BBa_K808000
sequence: https://synbiohub.org/public/igem/BBa_K808034_sequence/1

BBa_K808000
type: Component
persistent identity: component2201271
display id: component2201271
version: 1
top level: BBa_K808034
access: public
definition: Ara

BBa_J61101
type: Component
persistent identity: component2201272
display id: component2201272
version: 1
top level: BBa_K808034
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_K808001
type: Component
persistent identity: component2201273
display id: component2201273
version: 1
top level: BBa_K808034
access: public
definition: https://synbiohub.org/public/igem/BBa_K808001/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2201272
display id: annotation2201272
version: 1
top level: BBa_K808034
location: https://synbiohub.org/public/igem/BBa_K808034/annotation2201272/range2201272/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K808034/annotation2201272/range2201272/1
type: Range
persistent identity: range2201272
display id: range2201272
version: 1
direction: direction/reverse
top level: BBa_K808034
start: 1218
end: 1229
orientation: inline

BBa_K808001
type: SequenceAnnotation
persistent identity: annotation2201273
display id: annotation2201273
version: 1
top level: BBa_K808034
location: https://synbiohub.org/public/igem/BBa_K808034/annotation2201273/range2201273/1
component: BBa_K808001

https://synbiohub.org/public/igem/BBa_K808034/annotation2201273/range2201273/1
type: Range
persistent identity: range2201273
display id: range2201273
version: 1
direction: direction/reverse
top level: BBa_K808034
start: 1236
end: 2204
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2201271
display id: annotation2201271
version: 1
top level: BBa_K808034
location: https://synbiohub.org/public/igem/BBa_K808034/annotation2201271/range2201271/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K808034/annotation2201271/range2201271/1
type: Range
persistent identity: range2201271
display id: range2201271
version: 1
direction: direction/reverse
top level: BBa_K808034
start: 1
end: 1209
orientation: inline

BBa_K747078
type: ComponentDefinition
persistent identity: BBa K747078
display id: BBa_K747078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 5
sequence: https://synbiohub.org/public/igem/BBa_K747078_sequence/1

TG 5
type: SequenceAnnotation
persistent identity: annotation2197047
display id: annotation2197047
version: 1
top level: BBa_K747078
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747078/annotation2197047/range2197047/1

https://synbiohub.org/public/igem/BBa_K747078/annotation2197047/range2197047/1
type: Range
persistent identity: range2197047
display id: range2197047
version: 1
direction: direction/forward
top level: BBa_K747078
start: 14
end: 211
orientation: inline

BBa_K849003
type: ComponentDefinition
persistent identity: BBa K849003
display id: BBa_K849003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bifunctional Cyclase from Gibberella fujikuroi
creator: Martin Schneider, Sebastian Roy
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1109_
m user id: 0
owner id: 12313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: CPS/KS
mutable notes: no
mutable provenance: Genomic sequence of Gibberella fujikuroi.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849003_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202327
display id: annotation2202327
version: 1
top level: BBa_K849003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849003/annotation2202327/range2202327/1

https://synbiohub.org/public/igem/BBa_K849003/annotation2202327/range2202327/1
type: Range
persistent identity: range2202327
display id: range2202327
version: 1
direction: direction/forward
top level: BBa_K849003
start: 1
end: 2859
orientation: inline

BBa_K876069
type: ComponentDefinition
persistent identity: BBa K876069
display id: BBa_K876069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Term-pLuxR-TetR
creator: Zakaraya Ashour, Sanket Shah
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TT-pLuxR-TetR
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0040
component: BBa_B0015
component: BBa_R0062
sequence annotation: BBa_B0015
sequence annotation: BBa_R0062
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K876069_sequence/1

BBa_C0040
type: Component
persistent identity: component2251329
display id: component2251329
version: 1
top level: BBa_K876069
access: public
definition: tetR

BBa_B0015
type: Component
persistent identity: component2251320
display id: component2251320
version: 1
top level: BBa_K876069
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0062
type: Component
persistent identity: component2251322
display id: component2251322
version: 1
top level: BBa_K876069
access: public
definition: lux pR

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251320
display id: annotation2251320
version: 1
top level: BBa_K876069
location: https://synbiohub.org/public/igem/BBa_K876069/annotation2251320/range2251320/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876069/annotation2251320/range2251320/1
type: Range
persistent identity: range2251320
display id: range2251320
version: 1
direction: direction/reverse
top level: BBa_K876069
start: 1
end: 129
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2251322
display id: annotation2251322
version: 1
top level: BBa_K876069
location: https://synbiohub.org/public/igem/BBa_K876069/annotation2251322/range2251322/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K876069/annotation2251322/range2251322/1
type: Range
persistent identity: range2251322
display id: range2251322
version: 1
direction: direction/forward
top level: BBa_K876069
start: 138
end: 192
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2251329
display id: annotation2251329
version: 1
top level: BBa_K876069
location: https://synbiohub.org/public/igem/BBa_K876069/annotation2251329/range2251329/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876069/annotation2251329/range2251329/1
type: Range
persistent identity: range2251329
display id: range2251329
version: 1
direction: direction/reverse
top level: BBa_K876069
start: 199
end: 883
orientation: inline

BBa_K598019
type: ComponentDefinition
persistent identity: BBa K598019
display id: BBa_K598019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
creator: Zhenrun ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
mutable notes: the Ribosomal Binding Site of vioE is contained in TPP Ribozyme.
mutable provenance: Hammerhead Ribozyme backbone comes from E.coli, and TPP aptamer comes from B.subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: pBAD
sequence annotation: rbs
sequence annotation: TPP ribozyme 2.5
sequence annotation: vioB
sequence annotation: B0015
sequence annotation: vioD
sequence annotation: vioE
sequence annotation: vioA
sequence: https://synbiohub.org/public/igem/BBa_K598019_sequence/1

pBAD
type: SequenceAnnotation
persistent identity: annotation2144602
display id: annotation2144602
version: 1
top level: BBa_K598019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144602/range2144602/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144602/range2144602/1
type: Range
persistent identity: range2144602
display id: range2144602
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5849
end: 5978
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144630
display id: annotation2144630
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144630/range2144630/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144630/range2144630/1
type: Range
persistent identity: range2144630
display id: range2144630
version: 1
direction: direction/forward
top level: BBa_K598019
start: 6122
end: 6129
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144598
display id: annotation2144598
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144598/range2144598/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144598/range2144598/1
type: Range
persistent identity: range2144598
display id: range2144598
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1545
end: 1551
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144596
display id: annotation2144596
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144596/range2144596/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144596/range2144596/1
type: Range
persistent identity: range2144596
display id: range2144596
version: 1
direction: direction/forward
top level: BBa_K598019
start: 273
end: 279
orientation: inline

TPP ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2144624
display id: annotation2144624
version: 1
top level: BBa_K598019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144624/range2144624/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144624/range2144624/1
type: Range
persistent identity: range2144624
display id: range2144624
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5985
end: 6134
orientation: inline

vioB
type: SequenceAnnotation
persistent identity: annotation2144599
display id: annotation2144599
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144599/range2144599/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144599/range2144599/1
type: Range
persistent identity: range2144599
display id: range2144599
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1559
end: 4556
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2144595
display id: annotation2144595
version: 1
top level: BBa_K598019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144595/range2144595/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144595/range2144595/1
type: Range
persistent identity: range2144595
display id: range2144595
version: 1
direction: direction/forward
top level: BBa_K598019
start: 138
end: 267
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2144601
display id: annotation2144601
version: 1
top level: BBa_K598019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144601/range2144601/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144601/range2144601/1
type: Range
persistent identity: range2144601
display id: range2144601
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5712
end: 5841
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2144600
display id: annotation2144600
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144600/range2144600/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144600/range2144600/1
type: Range
persistent identity: range2144600
display id: range2144600
version: 1
direction: direction/forward
top level: BBa_K598019
start: 4579
end: 5704
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2144631
display id: annotation2144631
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144631/range2144631/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144631/range2144631/1
type: Range
persistent identity: range2144631
display id: range2144631
version: 1
direction: direction/forward
top level: BBa_K598019
start: 6135
end: 6710
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144594
display id: annotation2144594
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144594/range2144594/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144594/range2144594/1
type: Range
persistent identity: range2144594
display id: range2144594
version: 1
direction: direction/forward
top level: BBa_K598019
start: 4565
end: 4571
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2144593
display id: annotation2144593
version: 1
top level: BBa_K598019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144593/range2144593/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144593/range2144593/1
type: Range
persistent identity: range2144593
display id: range2144593
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1
end: 129
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2144597
display id: annotation2144597
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144597/range2144597/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144597/range2144597/1
type: Range
persistent identity: range2144597
display id: range2144597
version: 1
direction: direction/forward
top level: BBa_K598019
start: 285
end: 1542
orientation: inline

BBa_K849004
type: ComponentDefinition
persistent identity: BBa K849004
display id: BBa_K849004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ent-Kaurene Oxidase from Gibberella fujikuroi
creator: Martin Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
group u list: _1109_
m user id: 0
owner id: 12313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: KO
mutable notes: no
mutable provenance: Genomic Sequence from Gibberella fujikuroi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849004_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202568
display id: annotation2202568
version: 1
top level: BBa_K849004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849004/annotation2202568/range2202568/1

https://synbiohub.org/public/igem/BBa_K849004/annotation2202568/range2202568/1
type: Range
persistent identity: range2202568
display id: range2202568
version: 1
direction: direction/forward
top level: BBa_K849004
start: 1
end: 1578
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL-making Enzyme, luxI (+RBS)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0034.C0061
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
access: public
definition: luxI

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
direction: direction/forward
top level: BBa_C0261
start: 19
end: 636
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
direction: direction/forward
top level: BBa_C0261
start: 1
end: 12
orientation: inline

BBa_K649303
type: ComponentDefinition
persistent identity: BBa K649303
display id: BBa_K649303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlacIQ-RBS-ispS
creator: Goshi Sugano
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:13:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _826_
m user id: 0
owner id: 9613
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: RBS+ispS is same BBa_K649400. PlacIQ was used the part BBa_.
mutable notes: None.
mutable provenance: RBS+ispS : GeneArt Product PlacIQ : Part(BBa_)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K649400
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_K649400
sequence: https://synbiohub.org/public/igem/BBa_K649303_sequence/1

BBa_K649400
type: Component
persistent identity: component2146399
display id: component2146399
version: 1
top level: BBa_K649303
access: public
definition: https://synbiohub.org/public/igem/BBa_K649400/1

BBa_I14032
type: Component
persistent identity: component2146397
display id: component2146397
version: 1
top level: BBa_K649303
access: public
definition: https://synbiohub.org/public/igem/BBa_I14032/1

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2146397
display id: annotation2146397
version: 1
top level: BBa_K649303
location: https://synbiohub.org/public/igem/BBa_K649303/annotation2146397/range2146397/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K649303/annotation2146397/range2146397/1
type: Range
persistent identity: range2146397
display id: range2146397
version: 1
direction: direction/reverse
top level: BBa_K649303
start: 1
end: 37
orientation: inline

BBa_K649400
type: SequenceAnnotation
persistent identity: annotation2146399
display id: annotation2146399
version: 1
top level: BBa_K649303
location: https://synbiohub.org/public/igem/BBa_K649303/annotation2146399/range2146399/1
component: BBa_K649400

https://synbiohub.org/public/igem/BBa_K649303/annotation2146399/range2146399/1
type: Range
persistent identity: range2146399
display id: range2146399
version: 1
direction: direction/reverse
top level: BBa_K649303
start: 46
end: 1908
orientation: inline

BBa_K747081
type: ComponentDefinition
persistent identity: BBa K747081
display id: BBa_K747081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 5
sequence: https://synbiohub.org/public/igem/BBa_K747081_sequence/1

AC 5
type: SequenceAnnotation
persistent identity: annotation2197053
display id: annotation2197053
version: 1
top level: BBa_K747081
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747081/annotation2197053/range2197053/1

https://synbiohub.org/public/igem/BBa_K747081/annotation2197053/range2197053/1
type: Range
persistent identity: range2197053
display id: range2197053
version: 1
direction: direction/forward
top level: BBa_K747081
start: 14
end: 211
orientation: inline

tctA_503aa
type: ComponentDefinition
persistent identity: BBa K808002
display id: BBa_K808002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tctA_503: large subunit A1 of the tripartite tricarboxylate transporter family
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 13606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tctA_503: large subunit A1 of the tripartite tricarboxylate transporter family
mutable notes: .
mutable provenance: Comamonas tesosteroni KF1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808002_sequence/1

BBa_K747060
type: ComponentDefinition
persistent identity: BBa K747060
display id: BBa_K747060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 4
sequence: https://synbiohub.org/public/igem/BBa_K747060_sequence/1

TA 4
type: SequenceAnnotation
persistent identity: annotation2197016
display id: annotation2197016
version: 1
top level: BBa_K747060
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747060/annotation2197016/range2197016/1

https://synbiohub.org/public/igem/BBa_K747060/annotation2197016/range2197016/1
type: Range
persistent identity: range2197016
display id: range2197016
version: 1
direction: direction/forward
top level: BBa_K747060
start: 14
end: 211
orientation: inline

BBa_K801010
type: ComponentDefinition
persistent identity: BBa K801010
display id: BBa_K801010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF2 constitutive yeast promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive yeast promoter of medium strength compared to TEF1 promoter.
mutable notes: -
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TEF1-Promoter
sequence: https://synbiohub.org/public/igem/BBa_K801010_sequence/1

TEF1-Promoter
type: SequenceAnnotation
persistent identity: annotation2202139
display id: annotation2202139
version: 1
top level: BBa_K801010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801010/annotation2202139/range2202139/1

https://synbiohub.org/public/igem/BBa_K801010/annotation2202139/range2202139/1
type: Range
persistent identity: range2202139
display id: range2202139
version: 1
direction: direction/forward
top level: BBa_K801010
start: 1
end: 647
orientation: inline

BBa_K876073
type: ComponentDefinition
persistent identity: BBa K876073
display id: BBa_K876073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTetR-LasR-Term-Term-pLasR-LuxS-RBS-CFP-Term-Term-pLuxR-TetR
creator: Zakaraya Ashour, Sanket Shah
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pTetR-LasR-TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_R0079
component: BBa_E0422
component: BBa_K091109
component: BBa_C0040
component: BBa_K876057
sequence annotation: BBa_E0422
sequence annotation: BBa_R0062
sequence annotation: BBa_K876057
sequence annotation: BBa_C0040
sequence annotation: BBa_R0079
sequence annotation: BBa_K091109
sequence: https://synbiohub.org/public/igem/BBa_K876073_sequence/1

BBa_R0062
type: Component
persistent identity: component2298687
display id: component2298687
version: 1
top level: BBa_K876073
access: public
definition: lux pR

BBa_R0079
type: Component
persistent identity: component2298670
display id: component2298670
version: 1
top level: BBa_K876073
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_E0422
type: Component
persistent identity: component2298685
display id: component2298685
version: 1
top level: BBa_K876073
access: public
definition: BBa_E0422

BBa_K091109
type: Component
persistent identity: component2298671
display id: component2298671
version: 1
top level: BBa_K876073
access: public
definition: https://synbiohub.org/public/igem/BBa_K091109/1

BBa_C0040
type: Component
persistent identity: component2298694
display id: component2298694
version: 1
top level: BBa_K876073
access: public
definition: tetR

BBa_K876057
type: Component
persistent identity: component2298665
display id: component2298665
version: 1
top level: BBa_K876073
access: public
definition: BBa_K876057

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2298685
display id: annotation2298685
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298685/range2298685/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_K876073/annotation2298685/range2298685/1
type: Range
persistent identity: range2298685
display id: range2298685
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 1674
end: 2590
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2298687
display id: annotation2298687
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298687/range2298687/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K876073/annotation2298687/range2298687/1
type: Range
persistent identity: range2298687
display id: range2298687
version: 1
direction: direction/forward
top level: BBa_K876073
start: 2599
end: 2653
orientation: inline

BBa_K876057
type: SequenceAnnotation
persistent identity: annotation2298665
display id: annotation2298665
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298665/range2298665/1
component: BBa_K876057

https://synbiohub.org/public/igem/BBa_K876073/annotation2298665/range2298665/1
type: Range
persistent identity: range2298665
display id: range2298665
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 1
end: 978
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2298694
display id: annotation2298694
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298694/range2298694/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876073/annotation2298694/range2298694/1
type: Range
persistent identity: range2298694
display id: range2298694
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 2660
end: 3344
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2298670
display id: annotation2298670
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298670/range2298670/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K876073/annotation2298670/range2298670/1
type: Range
persistent identity: range2298670
display id: range2298670
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 987
end: 1143
orientation: inline

BBa_K091109
type: SequenceAnnotation
persistent identity: annotation2298671
display id: annotation2298671
version: 1
top level: BBa_K876073
location: https://synbiohub.org/public/igem/BBa_K876073/annotation2298671/range2298671/1
component: BBa_K091109

https://synbiohub.org/public/igem/BBa_K876073/annotation2298671/range2298671/1
type: Range
persistent identity: range2298671
display id: range2298671
version: 1
direction: direction/reverse
top level: BBa_K876073
start: 1150
end: 1665
orientation: inline

BBa_K747004
type: ComponentDefinition
persistent identity: BBa K747004
display id: BBa_K747004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CA1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CA 1
sequence: https://synbiohub.org/public/igem/BBa_K747004_sequence/1

CA 1
type: SequenceAnnotation
persistent identity: annotation2195787
display id: annotation2195787
version: 1
top level: BBa_K747004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747004/annotation2195787/range2195787/1

https://synbiohub.org/public/igem/BBa_K747004/annotation2195787/range2195787/1
type: Range
persistent identity: range2195787
display id: range2195787
version: 1
direction: direction/forward
top level: BBa_K747004
start: 12
end: 209
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from E. coli phage lambda (+LVA)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
direction: direction/forward
top level: cI lam
start: 712
end: 744
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
direction: direction/forward
top level: cI lam
start: 4
end: 711
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
direction: direction/forward
top level: cI lam
start: 751
end: 775
orientation: inline

BBa_K747055
type: ComponentDefinition
persistent identity: BBa K747055
display id: BBa_K747055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 4
sequence: https://synbiohub.org/public/igem/BBa_K747055_sequence/1

CT 4
type: SequenceAnnotation
persistent identity: annotation2197012
display id: annotation2197012
version: 1
top level: BBa_K747055
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747055/annotation2197012/range2197012/1

https://synbiohub.org/public/igem/BBa_K747055/annotation2197012/range2197012/1
type: Range
persistent identity: range2197012
display id: range2197012
version: 1
direction: direction/forward
top level: BBa_K747055
start: 14
end: 211
orientation: inline

BBa_K747023
type: ComponentDefinition
persistent identity: BBa K747023
display id: BBa_K747023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 2
sequence: https://synbiohub.org/public/igem/BBa_K747023_sequence/1

CT 2
type: SequenceAnnotation
persistent identity: annotation2196054
display id: annotation2196054
version: 1
top level: BBa_K747023
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747023/annotation2196054/range2196054/1

https://synbiohub.org/public/igem/BBa_K747023/annotation2196054/range2196054/1
type: Range
persistent identity: range2196054
display id: range2196054
version: 1
direction: direction/forward
top level: BBa_K747023
start: 14
end: 211
orientation: inline

BBa_J13023
type: ComponentDefinition
persistent identity: BBa J13023
display id: BBa_J13023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL+LuxR dependent POPS/RIPS generator
creator: Jeff Tabor
created: 2005-06-28T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: produces pops and rips in the presence of luxR/3OC6HSL and the absence of lambda cI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0065
component: BBa_B0034
sequence annotation: BBa_R0065
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13023_sequence/1

BBa_R0065
type: Component
persistent identity: component1556571
display id: component1556571
version: 1
top level: BBa_J13023
access: public
definition: cI+luxR

BBa_B0034
type: Component
persistent identity: component1556583
display id: component1556583
version: 1
top level: BBa_J13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1556571
display id: annotation1556571
version: 1
top level: BBa_J13023
location: https://synbiohub.org/public/igem/BBa_J13023/annotation1556571/range1556571/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_J13023/annotation1556571/range1556571/1
type: Range
persistent identity: range1556571
display id: range1556571
version: 1
direction: direction/forward
top level: BBa_J13023
start: 1
end: 97
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1556583
display id: annotation1556583
version: 1
top level: BBa_J13023
location: https://synbiohub.org/public/igem/BBa_J13023/annotation1556583/range1556583/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13023/annotation1556583/range1556583/1
type: Range
persistent identity: range1556583
display id: range1556583
version: 1
direction: direction/forward
top level: BBa_J13023
start: 106
end: 117
orientation: inline

BBa_K741008
type: ComponentDefinition
persistent identity: BBa K741008
display id: BBa_K741008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCon(0.244)-RBS-crogfp-T
creator: Wuyang Chen
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Here, pCon(J23114) constantly expressing the fusion protein crogfp at 10% of the strength of pCon(J23100).
mutable notes: TBD
mutable provenance: BBa_J23114 BBa_K741006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23105
component: BBa_K741006
sequence annotation: BBa_K741006
sequence annotation: BBa_J23105
sequence: https://synbiohub.org/public/igem/BBa_K741008_sequence/1

BBa_J23105
type: Component
persistent identity: component2223132
display id: component2223132
version: 1
top level: BBa_K741008
access: public
definition: https://synbiohub.org/public/igem/BBa_J23105/1

BBa_K741006
type: Component
persistent identity: component2223143
display id: component2223143
version: 1
top level: BBa_K741008
access: public
definition: https://synbiohub.org/public/igem/BBa_K741006/1

BBa_K741006
type: SequenceAnnotation
persistent identity: annotation2223143
display id: annotation2223143
version: 1
top level: BBa_K741008
location: https://synbiohub.org/public/igem/BBa_K741008/annotation2223143/range2223143/1
component: BBa_K741006

https://synbiohub.org/public/igem/BBa_K741008/annotation2223143/range2223143/1
type: Range
persistent identity: range2223143
display id: range2223143
version: 1
direction: direction/reverse
top level: BBa_K741008
start: 44
end: 1131
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2223132
display id: annotation2223132
version: 1
top level: BBa_K741008
location: https://synbiohub.org/public/igem/BBa_K741008/annotation2223132/range2223132/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K741008/annotation2223132/range2223132/1
type: Range
persistent identity: range2223132
display id: range2223132
version: 1
direction: direction/reverse
top level: BBa_K741008
start: 1
end: 35
orientation: inline

BBa_K747022
type: ComponentDefinition
persistent identity: BBa K747022
display id: BBa_K747022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 2
sequence: https://synbiohub.org/public/igem/BBa_K747022_sequence/1

CG 2
type: SequenceAnnotation
persistent identity: annotation2196045
display id: annotation2196045
version: 1
top level: BBa_K747022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747022/annotation2196045/range2196045/1

https://synbiohub.org/public/igem/BBa_K747022/annotation2196045/range2196045/1
type: Range
persistent identity: range2196045
display id: range2196045
version: 1
direction: direction/forward
top level: BBa_K747022
start: 14
end: 211
orientation: inline

BBa_K779503
type: ComponentDefinition
persistent identity: BBa K779503
display id: BBa_K779503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long input strand MammoBlock
creator: Kristjan Eerik Kaseniit
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1033_
m user id: 0
owner id: 12684
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Long matching input strand to the reporter formed by parts Part:BBa K779500 and Part:BBa K779502 or Part:BBa K779501 and Part:BBa K779502. Modifications: 5' IRD800 fluorophore, 3' phosphate. IDT DNA oligo order: /5IRD800/mCmA mCmCmC mAmCmU mCmCmU mCmUmC mCmCmA mCmCmA mAmCmU mAmUmC mCmA/3Phos/
mutable notes: 3' phosphate added to protect from some 3' exonucleases (suggested by IDT). See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
mutable provenance: De novo design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Domain Sa
sequence annotation: Toehold
sequence: https://synbiohub.org/public/igem/BBa_K779503_sequence/1

Domain Sa
type: SequenceAnnotation
persistent identity: annotation2204255
display id: annotation2204255
version: 1
top level: BBa_K779503
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779503/annotation2204255/range2204255/1

https://synbiohub.org/public/igem/BBa_K779503/annotation2204255/range2204255/1
type: Range
persistent identity: range2204255
display id: range2204255
version: 1
direction: direction/forward
top level: BBa_K779503
start: 1
end: 20
orientation: inline

Toehold
type: SequenceAnnotation
persistent identity: annotation2204256
display id: annotation2204256
version: 1
top level: BBa_K779503
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779503/annotation2204256/range2204256/1

https://synbiohub.org/public/igem/BBa_K779503/annotation2204256/range2204256/1
type: Range
persistent identity: range2204256
display id: range2204256
version: 1
direction: direction/forward
top level: BBa_K779503
start: 21
end: 28
orientation: inline

BBa_K747009
type: ComponentDefinition
persistent identity: BBa K747009
display id: BBa_K747009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 1
sequence: https://synbiohub.org/public/igem/BBa_K747009_sequence/1

GC 1
type: SequenceAnnotation
persistent identity: annotation2195828
display id: annotation2195828
version: 1
top level: BBa_K747009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747009/annotation2195828/range2195828/1

https://synbiohub.org/public/igem/BBa_K747009/annotation2195828/range2195828/1
type: Range
persistent identity: range2195828
display id: range2195828
version: 1
direction: direction/forward
top level: BBa_K747009
start: 12
end: 209
orientation: inline

BBa_I6086
type: ComponentDefinition
persistent identity: BBa I6086
display id: BBa_I6086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1062.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0032
component: BBa_B0010
component: BBa_R1062
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_R1062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6086_sequence/1

BBa_E0032
type: Component
persistent identity: component949612
display id: component949612
version: 1
top level: BBa_I6086
access: public
definition: YFP

BBa_B0010
type: Component
persistent identity: component949620
display id: component949620
version: 1
top level: BBa_I6086
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R1062
type: Component
persistent identity: component949592
display id: component949592
version: 1
top level: BBa_I6086
access: public
definition: lux pR

BBa_B0034
type: Component
persistent identity: component949600
display id: component949600
version: 1
top level: BBa_I6086
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component949630
display id: component949630
version: 1
top level: BBa_I6086
access: public
definition: BBa_B0012

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation949592
display id: annotation949592
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949592/range949592/1
component: BBa_R1062

https://synbiohub.org/public/igem/BBa_I6086/annotation949592/range949592/1
type: Range
persistent identity: range949592
display id: range949592
version: 1
direction: direction/forward
top level: BBa_I6086
start: 1
end: 56
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949630
display id: annotation949630
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949630/range949630/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6086/annotation949630/range949630/1
type: Range
persistent identity: range949630
display id: range949630
version: 1
direction: direction/forward
top level: BBa_I6086
start: 941
end: 981
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949600
display id: annotation949600
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949600/range949600/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6086/annotation949600/range949600/1
type: Range
persistent identity: range949600
display id: range949600
version: 1
direction: direction/forward
top level: BBa_I6086
start: 65
end: 76
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949612
display id: annotation949612
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949612/range949612/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6086/annotation949612/range949612/1
type: Range
persistent identity: range949612
display id: range949612
version: 1
direction: direction/forward
top level: BBa_I6086
start: 83
end: 844
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949620
display id: annotation949620
version: 1
top level: BBa_I6086
location: https://synbiohub.org/public/igem/BBa_I6086/annotation949620/range949620/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6086/annotation949620/range949620/1
type: Range
persistent identity: range949620
display id: range949620
version: 1
direction: direction/forward
top level: BBa_I6086
start: 853
end: 932
orientation: inline

BBa_K909008
type: ComponentDefinition
persistent identity: BBa K909008
display id: BBa_K909008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR-DBD-UVR8 fusion protein
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline repressor DNA binding domain fusion with truncated version of UVR8 (tetR-DBD-UVR8). UVR8 is a UV-B sensing receptor in plants necessary for UV-B protection. In dark state UVR8 forms a dimer which is monomerized by the UV-B light (280-315 nm). A truncated version of UVR8 (14-440 amino acids) was fused with tetR DNA binding domain (tetR-DBD), lacking of dimerization domain. Such mutant shows no repression of Ptet promoter, however fusion with dimerizing protein (UVR8) returns tetR-DBD activity. Later UV-B exposure breaks UVR8 dimer and releases tetR-DBD-UVR8 from Ptet, activating transcription. Thus, this tetR-DBD-UVR8 can be used as a one step UV-B on switch.
mutable notes: Contains two illegal PstI sites in UVR8 coding region and BamHI site was used for protein fusion
mutable provenance: tetR-DBD was cloned from E.coli tetracyclin repressor tetR from transposon Tn10 cDNA of UVR8 was provided by Roman Ulm, University of Geneva.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR-DBD
sequence annotation: UVR8
sequence: https://synbiohub.org/public/igem/BBa_K909008_sequence/1

tetR-DBD
type: SequenceAnnotation
persistent identity: annotation2194628
display id: annotation2194628
version: 1
top level: BBa_K909008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909008/annotation2194628/range2194628/1

https://synbiohub.org/public/igem/BBa_K909008/annotation2194628/range2194628/1
type: Range
persistent identity: range2194628
display id: range2194628
version: 1
direction: direction/forward
top level: BBa_K909008
start: 1
end: 390
orientation: inline

UVR8
type: SequenceAnnotation
persistent identity: annotation2194629
display id: annotation2194629
version: 1
top level: BBa_K909008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909008/annotation2194629/range2194629/1

https://synbiohub.org/public/igem/BBa_K909008/annotation2194629/range2194629/1
type: Range
persistent identity: range2194629
display id: range2194629
version: 1
direction: direction/forward
top level: BBa_K909008
start: 391
end: 1674
orientation: inline

BBa_K747073
type: ComponentDefinition
persistent identity: BBa K747073
display id: BBa_K747073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 5
sequence: https://synbiohub.org/public/igem/BBa_K747073_sequence/1

GC 5
type: SequenceAnnotation
persistent identity: annotation2197041
display id: annotation2197041
version: 1
top level: BBa_K747073
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747073/annotation2197041/range2197041/1

https://synbiohub.org/public/igem/BBa_K747073/annotation2197041/range2197041/1
type: Range
persistent identity: range2197041
display id: range2197041
version: 1
direction: direction/forward
top level: BBa_K747073
start: 14
end: 211
orientation: inline

BBa_K876038
type: ComponentDefinition
persistent identity: BBa K876038
display id: BBa_K876038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TT-pLasR-araC
creator: Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TT-pLasR-araC
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K876044
sequence annotation: BBa_B0015
sequence annotation: BBa_K876044
sequence: https://synbiohub.org/public/igem/BBa_K876038_sequence/1

BBa_B0015
type: Component
persistent identity: component2251249
display id: component2251249
version: 1
top level: BBa_K876038
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K876044
type: Component
persistent identity: component2251259
display id: component2251259
version: 1
top level: BBa_K876038
access: public
definition: https://synbiohub.org/public/igem/BBa_K876044/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251249
display id: annotation2251249
version: 1
top level: BBa_K876038
location: https://synbiohub.org/public/igem/BBa_K876038/annotation2251249/range2251249/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876038/annotation2251249/range2251249/1
type: Range
persistent identity: range2251249
display id: range2251249
version: 1
direction: direction/reverse
top level: BBa_K876038
start: 1
end: 129
orientation: inline

BBa_K876044
type: SequenceAnnotation
persistent identity: annotation2251259
display id: annotation2251259
version: 1
top level: BBa_K876038
location: https://synbiohub.org/public/igem/BBa_K876038/annotation2251259/range2251259/1
component: BBa_K876044

https://synbiohub.org/public/igem/BBa_K876038/annotation2251259/range2251259/1
type: Range
persistent identity: range2251259
display id: range2251259
version: 1
direction: direction/reverse
top level: BBa_K876038
start: 138
end: 1240
orientation: inline

BBa_K777113
type: ComponentDefinition
persistent identity: BBa K777113
display id: BBa_K777113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA
creator: Team Goettingen
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The motA gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGACCGGTGGAAGCCTTGG **Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATGCTTCCTCGGTTGTCG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MotA coding sequence
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K777113_sequence/1

MotA coding sequence
type: SequenceAnnotation
persistent identity: annotation2184142
display id: annotation2184142
version: 1
top level: BBa_K777113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184142/range2184142/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184142/range2184142/1
type: Range
persistent identity: range2184142
display id: range2184142
version: 1
direction: direction/forward
top level: BBa_K777113
start: 1
end: 831
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2184143
display id: annotation2184143
version: 1
top level: BBa_K777113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184143/range2184143/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184143/range2184143/1
type: Range
persistent identity: range2184143
display id: range2184143
version: 1
direction: direction/forward
top level: BBa_K777113
start: 1
end: 1
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2184144
display id: annotation2184144
version: 1
top level: BBa_K777113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184144/range2184144/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184144/range2184144/1
type: Range
persistent identity: range2184144
display id: range2184144
version: 1
direction: direction/forward
top level: BBa_K777113
start: 831
end: 831
orientation: inline

BBa_I6107
type: ComponentDefinition
persistent identity: BBa I6107
display id: BBa_I6107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0079.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0010
component: BBa_E0030
component: BBa_B0012
component: BBa_R0079
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0079
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I6107_sequence/1

BBa_B0034
type: Component
persistent identity: component949924
display id: component949924
version: 1
top level: BBa_I6107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component949931
display id: component949931
version: 1
top level: BBa_I6107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component949926
display id: component949926
version: 1
top level: BBa_I6107
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component949941
display id: component949941
version: 1
top level: BBa_I6107
access: public
definition: BBa_B0012

BBa_R0079
type: Component
persistent identity: component949919
display id: component949919
version: 1
top level: BBa_I6107
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949924
display id: annotation949924
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949924/range949924/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6107/annotation949924/range949924/1
type: Range
persistent identity: range949924
display id: range949924
version: 1
direction: direction/forward
top level: BBa_I6107
start: 166
end: 177
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949941
display id: annotation949941
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949941/range949941/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6107/annotation949941/range949941/1
type: Range
persistent identity: range949941
display id: range949941
version: 1
direction: direction/forward
top level: BBa_I6107
start: 1003
end: 1043
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949931
display id: annotation949931
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949931/range949931/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6107/annotation949931/range949931/1
type: Range
persistent identity: range949931
display id: range949931
version: 1
direction: direction/forward
top level: BBa_I6107
start: 915
end: 994
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation949919
display id: annotation949919
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949919/range949919/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_I6107/annotation949919/range949919/1
type: Range
persistent identity: range949919
display id: range949919
version: 1
direction: direction/forward
top level: BBa_I6107
start: 1
end: 157
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949926
display id: annotation949926
version: 1
top level: BBa_I6107
location: https://synbiohub.org/public/igem/BBa_I6107/annotation949926/range949926/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6107/annotation949926/range949926/1
type: Range
persistent identity: range949926
display id: range949926
version: 1
direction: direction/forward
top level: BBa_I6107
start: 184
end: 906
orientation: inline

trkD
type: ComponentDefinition
persistent identity: BBa K629005
display id: BBa_K629005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
creator: Zilong WANG, Yi ZHENG
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
mutable notes: none
mutable provenance: ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TrkD
sequence annotation: start
sequence annotation: fwd primer
sequence annotation: rev primer
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_K629005_sequence/1

TrkD
type: SequenceAnnotation
persistent identity: annotation2157756
display id: annotation2157756
version: 1
top level: trkD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1
type: Range
persistent identity: range2157756
display id: range2157756
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 1869
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2157754
display id: annotation2157754
version: 1
top level: trkD
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1
type: Range
persistent identity: range2157754
display id: range2157754
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 3
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2157755
display id: annotation2157755
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1
type: Range
persistent identity: range2157755
display id: range2157755
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 21
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2157757
display id: annotation2157757
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1
type: Range
persistent identity: range2157757
display id: range2157757
version: 1
direction: direction/reverse
top level: trkD
start: 1849
end: 1869
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2157758
display id: annotation2157758
version: 1
top level: trkD
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1
type: Range
persistent identity: range2157758
display id: range2157758
version: 1
direction: direction/forward
top level: trkD
start: 1867
end: 1869
orientation: inline

BBa_K731520
type: ComponentDefinition
persistent identity: BBa K731520
display id: BBa_K731520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible GFPmut3b
creator: Francesco Guzzonato
created: 2012-08-21T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12142
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control. lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.
mutable provenance: This construct has ben obtained from ligation between BBa_K731500 and BBa_E0240.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K731500
component: BBa_E0240
sequence annotation: BBa_E0240
sequence annotation: BBa_K731500
sequence: https://synbiohub.org/public/igem/BBa_K731520_sequence/1

BBa_K731500
type: Component
persistent identity: component2181555
display id: component2181555
version: 1
top level: BBa_K731520
access: public
definition: https://synbiohub.org/public/igem/BBa_K731500/1

BBa_E0240
type: Component
persistent identity: component2181566
display id: component2181566
version: 1
top level: BBa_K731520
access: public
definition: GFP report

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2181566
display id: annotation2181566
version: 1
top level: BBa_K731520
location: https://synbiohub.org/public/igem/BBa_K731520/annotation2181566/range2181566/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K731520/annotation2181566/range2181566/1
type: Range
persistent identity: range2181566
display id: range2181566
version: 1
direction: direction/reverse
top level: BBa_K731520
start: 1248
end: 2123
orientation: inline

BBa_K731500
type: SequenceAnnotation
persistent identity: annotation2181555
display id: annotation2181555
version: 1
top level: BBa_K731520
location: https://synbiohub.org/public/igem/BBa_K731520/annotation2181555/range2181555/1
component: BBa_K731500

https://synbiohub.org/public/igem/BBa_K731520/annotation2181555/range2181555/1
type: Range
persistent identity: range2181555
display id: range2181555
version: 1
direction: direction/reverse
top level: BBa_K731520
start: 1
end: 1239
orientation: inline

BBa_K784047
type: ComponentDefinition
persistent identity: BBa K784047
display id: BBa_K784047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline riboswitch + wt T7 RNAP
creator: Asaf Blumental
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part contains an engineered theophylline riboswitch (RS) ([http://partsregistry.org/Part:BBa_K784005 BBa K784005]) and T7 RNA polymerase ([http://partsregistry.org/Part:BBa_I2032 BBa I2032]). The riboswitch contains a RBS and it will be exposed and starts translating the T7 RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7 RNAP are adjacent to one another without any restriction site between them. There is an additional stop codon at the end of the gene.
mutable notes: The riboswitch had to fused to the RNAP using assembly PCR.
mutable provenance: Assembly PCR of [http://partsregistry.org/Part:BBa_K784005 BBa K784005] and [http://partsregistry.org/Part:BBa_I2032 BBa_I2032]. [http://partsregistry.org/Part:BBa_I2032 BBa I2032] was received from Ann K. Ganesan.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I2032
component: BBa_K784005
sequence annotation: BBa_K784005
sequence annotation: BBa_I2032
sequence: https://synbiohub.org/public/igem/BBa_K784047_sequence/1

BBa_I2032
type: Component
persistent identity: component2199521
display id: component2199521
version: 1
top level: BBa_K784047
access: public
definition: https://synbiohub.org/public/igem/BBa_I2032/1

BBa_K784005
type: Component
persistent identity: component2199519
display id: component2199519
version: 1
top level: BBa_K784047
access: public
definition: https://synbiohub.org/public/igem/BBa_K784005/1

BBa_K784005
type: SequenceAnnotation
persistent identity: annotation2199519
display id: annotation2199519
version: 1
top level: BBa_K784047
location: https://synbiohub.org/public/igem/BBa_K784047/annotation2199519/range2199519/1
component: BBa_K784005

https://synbiohub.org/public/igem/BBa_K784047/annotation2199519/range2199519/1
type: Range
persistent identity: range2199519
display id: range2199519
version: 1
direction: direction/reverse
top level: BBa_K784047
start: 1
end: 74
orientation: inline

BBa_I2032
type: SequenceAnnotation
persistent identity: annotation2199521
display id: annotation2199521
version: 1
top level: BBa_K784047
location: https://synbiohub.org/public/igem/BBa_K784047/annotation2199521/range2199521/1
component: BBa_I2032

https://synbiohub.org/public/igem/BBa_K784047/annotation2199521/range2199521/1
type: Range
persistent identity: range2199521
display id: range2199521
version: 1
direction: direction/reverse
top level: BBa_K784047
start: 75
end: 2729
orientation: inline

BBa_K876015
type: ComponentDefinition
persistent identity: BBa K876015
display id: BBa_K876015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-LasR-DT
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: true
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter for LasR which can be repressed by TetR
mutable notes: Biobrick placement
mutable provenance: Created from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_C0079
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_K876015_sequence/1

BBa_R0040
type: Component
persistent identity: component2251226
display id: component2251226
version: 1
top level: BBa_K876015
access: public
definition: p(tetR)

BBa_C0079
type: Component
persistent identity: component2251235
display id: component2251235
version: 1
top level: BBa_K876015
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_B0015
type: Component
persistent identity: component2251242
display id: component2251242
version: 1
top level: BBa_K876015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251242
display id: annotation2251242
version: 1
top level: BBa_K876015
location: https://synbiohub.org/public/igem/BBa_K876015/annotation2251242/range2251242/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876015/annotation2251242/range2251242/1
type: Range
persistent identity: range2251242
display id: range2251242
version: 1
direction: direction/reverse
top level: BBa_K876015
start: 850
end: 978
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251226
display id: annotation2251226
version: 1
top level: BBa_K876015
location: https://synbiohub.org/public/igem/BBa_K876015/annotation2251226/range2251226/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876015/annotation2251226/range2251226/1
type: Range
persistent identity: range2251226
display id: range2251226
version: 1
direction: direction/forward
top level: BBa_K876015
start: 1
end: 54
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2251235
display id: annotation2251235
version: 1
top level: BBa_K876015
location: https://synbiohub.org/public/igem/BBa_K876015/annotation2251235/range2251235/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K876015/annotation2251235/range2251235/1
type: Range
persistent identity: range2251235
display id: range2251235
version: 1
direction: direction/reverse
top level: BBa_K876015
start: 61
end: 841
orientation: inline

BBa_K747036
type: ComponentDefinition
persistent identity: BBa K747036
display id: BBa_K747036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 3
sequence: https://synbiohub.org/public/igem/BBa_K747036_sequence/1

AT 3
type: SequenceAnnotation
persistent identity: annotation2196214
display id: annotation2196214
version: 1
top level: BBa_K747036
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747036/annotation2196214/range2196214/1

https://synbiohub.org/public/igem/BBa_K747036/annotation2196214/range2196214/1
type: Range
persistent identity: range2196214
display id: range2196214
version: 1
direction: direction/forward
top level: BBa_K747036
start: 14
end: 211
orientation: inline

Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
mutable notes: non
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraO
sequence annotation: AraPromoter
sequence annotation: AraC
sequence annotation: AraO1
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
type: Range
persistent identity: range2200758
display id: range2200758
version: 1
direction: direction/forward
top level: Ara
start: 909
end: 924
orientation: inline

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
type: Range
persistent identity: range2200760
display id: range2200760
version: 1
direction: direction/forward
top level: Ara
start: 1153
end: 1181
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
type: Range
persistent identity: range2200757
display id: range2200757
version: 1
direction: direction/reverse
top level: Ara
start: 1
end: 879
orientation: inline

AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
type: Range
persistent identity: range2200759
display id: range2200759
version: 1
direction: direction/forward
top level: Ara
start: 1066
end: 1077
orientation: inline

BBa_K850000
type: ComponentDefinition
persistent identity: BBa K850000
display id: BBa_K850000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AGM1 gene from candida albicans
creator: Min-Gyu Kim
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
group u list: _1110_
m user id: 0
owner id: 10645
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phosphoacetylglucosamine mutase (N-acetylglucosamine-phosphate mutase); enzyme of UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis (1) This enzyme is part of the pathway to make the sugar subunits that make up the chitin polymer. This pathway was taken from yeast but has been shown to function in Acetobacter xylinum.
mutable notes: Gibson assembly fragment must have ovelapping sequences of about 50 bp.
mutable provenance: The sequence is from the candida genome database orf19.5013 (C. albicans SC5314. It contains a ribosome binding site. It was synthesized in <500bp pieces for Gibson Assembly and assembeled into pSB1C3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K850000_sequence/1

BBa_K812032
type: ComponentDefinition
persistent identity: BBa K812032
display id: BBa_K812032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCFP with kozak sequence for eukaryotic expression
creator: Chauris Karine
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1069_
m user id: 0
owner id: 12937
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts.
mutable notes: PCR and cloned in pSB1C3 using EcoRI and PstI
mutable provenance: PCR amplified with kozak sequence from DNA coming from Alfonso Jaramillo's lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCFP
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K812032_sequence/1

mCFP
type: SequenceAnnotation
persistent identity: annotation2187737
display id: annotation2187737
version: 1
top level: BBa_K812032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812032/annotation2187737/range2187737/1

https://synbiohub.org/public/igem/BBa_K812032/annotation2187737/range2187737/1
type: Range
persistent identity: range2187737
display id: range2187737
version: 1
direction: direction/forward
top level: BBa_K812032
start: 6
end: 722
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2187736
display id: annotation2187736
version: 1
top level: BBa_K812032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K812032/annotation2187736/range2187736/1

https://synbiohub.org/public/igem/BBa_K812032/annotation2187736/range2187736/1
type: Range
persistent identity: range2187736
display id: range2187736
version: 1
direction: direction/forward
top level: BBa_K812032
start: 1
end: 5
orientation: inline

BBa_K909005
type: ComponentDefinition
persistent identity: BBa K909005
display id: BBa_K909005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI protein with EYFP as a reporter
creator: Deborah Huber
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: sdkuhdshkudak
mutable notes: dsasad
mutable provenance: sddas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0430
component: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_E0430
sequence: https://synbiohub.org/public/igem/BBa_K909005_sequence/1

BBa_B0034
type: Component
persistent identity: component2264560
display id: component2264560
version: 1
top level: BBa_K909005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0430
type: Component
persistent identity: component2264574
display id: component2264574
version: 1
top level: BBa_K909005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_C0051
type: Component
persistent identity: component2264564
display id: component2264564
version: 1
top level: BBa_K909005
access: public
definition: cI lam

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2264560
display id: annotation2264560
version: 1
top level: BBa_K909005
location: https://synbiohub.org/public/igem/BBa_K909005/annotation2264560/range2264560/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909005/annotation2264560/range2264560/1
type: Range
persistent identity: range2264560
display id: range2264560
version: 1
direction: direction/reverse
top level: BBa_K909005
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2264564
display id: annotation2264564
version: 1
top level: BBa_K909005
location: https://synbiohub.org/public/igem/BBa_K909005/annotation2264564/range2264564/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K909005/annotation2264564/range2264564/1
type: Range
persistent identity: range2264564
display id: range2264564
version: 1
direction: direction/reverse
top level: BBa_K909005
start: 19
end: 793
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2264574
display id: annotation2264574
version: 1
top level: BBa_K909005
location: https://synbiohub.org/public/igem/BBa_K909005/annotation2264574/range2264574/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_K909005/annotation2264574/range2264574/1
type: Range
persistent identity: range2264574
display id: range2264574
version: 1
direction: direction/reverse
top level: BBa_K909005
start: 802
end: 1679
orientation: inline

BBa_I6076
type: ComponentDefinition
persistent identity: BBa I6076
display id: BBa_I6076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0065.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_E0032
component: BBa_B0010
component: BBa_B0012
component: BBa_R0065
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0032
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_I6076_sequence/1

BBa_B0034
type: Component
persistent identity: component949348
display id: component949348
version: 1
top level: BBa_I6076
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component949360
display id: component949360
version: 1
top level: BBa_I6076
access: public
definition: YFP

BBa_B0010
type: Component
persistent identity: component949368
display id: component949368
version: 1
top level: BBa_I6076
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949378
display id: component949378
version: 1
top level: BBa_I6076
access: public
definition: BBa_B0012

BBa_R0065
type: Component
persistent identity: component949336
display id: component949336
version: 1
top level: BBa_I6076
access: public
definition: cI+luxR

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949348
display id: annotation949348
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949348/range949348/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6076/annotation949348/range949348/1
type: Range
persistent identity: range949348
display id: range949348
version: 1
direction: direction/forward
top level: BBa_I6076
start: 106
end: 117
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949368
display id: annotation949368
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949368/range949368/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6076/annotation949368/range949368/1
type: Range
persistent identity: range949368
display id: range949368
version: 1
direction: direction/forward
top level: BBa_I6076
start: 894
end: 973
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949378
display id: annotation949378
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949378/range949378/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6076/annotation949378/range949378/1
type: Range
persistent identity: range949378
display id: range949378
version: 1
direction: direction/forward
top level: BBa_I6076
start: 982
end: 1022
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949360
display id: annotation949360
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949360/range949360/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6076/annotation949360/range949360/1
type: Range
persistent identity: range949360
display id: range949360
version: 1
direction: direction/forward
top level: BBa_I6076
start: 124
end: 885
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation949336
display id: annotation949336
version: 1
top level: BBa_I6076
location: https://synbiohub.org/public/igem/BBa_I6076/annotation949336/range949336/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_I6076/annotation949336/range949336/1
type: Range
persistent identity: range949336
display id: range949336
version: 1
direction: direction/forward
top level: BBa_I6076
start: 1
end: 97
orientation: inline

BBa_K863000
type: ComponentDefinition
persistent identity: BBa K863000
display id: BBa_K863000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: bpul (Laccase from Bacillus pumilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: RBS
sequence annotation: bpul
sequence annotation: 6 x HIS
sequence annotation: A (Original) T (Mutation)
sequence annotation: T7- Promotor BBa_I719005
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863000_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184915
display id: annotation2184915
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1
type: Range
persistent identity: range2184915
display id: range2184915
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1585
end: 1587
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2184909
display id: annotation2184909
version: 1
top level: BBa_K863000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1
type: Range
persistent identity: range2184909
display id: range2184909
version: 1
direction: direction/forward
top level: BBa_K863000
start: 25
end: 30
orientation: inline

bpul
type: SequenceAnnotation
persistent identity: annotation2184911
display id: annotation2184911
version: 1
top level: BBa_K863000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1
type: Range
persistent identity: range2184911
display id: range2184911
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 1556
orientation: inline

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2184913
display id: annotation2184913
version: 1
top level: BBa_K863000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1
type: Range
persistent identity: range2184913
display id: range2184913
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1557
end: 1581
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184914
display id: annotation2184914
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1
type: Range
persistent identity: range2184914
display id: range2184914
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1582
end: 1584
orientation: inline

A (Original) T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184912
display id: annotation2184912
version: 1
top level: BBa_K863000
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1
type: Range
persistent identity: range2184912
display id: range2184912
version: 1
direction: direction/forward
top level: BBa_K863000
start: 834
end: 834
orientation: inline

T7- Promotor BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2184908
display id: annotation2184908
version: 1
top level: BBa_K863000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1
type: Range
persistent identity: range2184908
display id: range2184908
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1
end: 23
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184910
display id: annotation2184910
version: 1
top level: BBa_K863000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1
type: Range
persistent identity: range2184910
display id: range2184910
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 36
orientation: inline

BBa_K747086
type: ComponentDefinition
persistent identity: BBa K747086
display id: BBa_K747086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 6
sequence: https://synbiohub.org/public/igem/BBa_K747086_sequence/1

CG 6
type: SequenceAnnotation
persistent identity: annotation2197060
display id: annotation2197060
version: 1
top level: BBa_K747086
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747086/annotation2197060/range2197060/1

https://synbiohub.org/public/igem/BBa_K747086/annotation2197060/range2197060/1
type: Range
persistent identity: range2197060
display id: range2197060
version: 1
direction: direction/forward
top level: BBa_K747086
start: 14
end: 211
orientation: inline

BBa_K775010
type: ComponentDefinition
persistent identity: BBa K775010
display id: BBa_K775010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Isoamylacetate Chimeric GPCR with Fus1-EGFP reporter (2)
creator: Sietske Grijseels
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K775004
component: BBa_K775001
sequence annotation: BBa_K775004
sequence annotation: BBa_K775001
sequence: https://synbiohub.org/public/igem/BBa_K775010_sequence/1

BBa_K775004
type: Component
persistent identity: component2195596
display id: component2195596
version: 1
top level: BBa_K775010
access: public
definition: https://synbiohub.org/public/igem/BBa_K775004/1

BBa_K775001
type: Component
persistent identity: component2195597
display id: component2195597
version: 1
top level: BBa_K775010
access: public
definition: BBa_K775001

BBa_K775004
type: SequenceAnnotation
persistent identity: annotation2195596
display id: annotation2195596
version: 1
top level: BBa_K775010
location: https://synbiohub.org/public/igem/BBa_K775010/annotation2195596/range2195596/1
component: BBa_K775004

https://synbiohub.org/public/igem/BBa_K775010/annotation2195596/range2195596/1
type: Range
persistent identity: range2195596
display id: range2195596
version: 1
direction: direction/reverse
top level: BBa_K775010
start: 1
end: 1166
orientation: inline

BBa_K775001
type: SequenceAnnotation
persistent identity: annotation2195597
display id: annotation2195597
version: 1
top level: BBa_K775010
location: https://synbiohub.org/public/igem/BBa_K775010/annotation2195597/range2195597/1
component: BBa_K775001

https://synbiohub.org/public/igem/BBa_K775010/annotation2195597/range2195597/1
type: Range
persistent identity: range2195597
display id: range2195597
version: 1
direction: direction/reverse
top level: BBa_K775010
start: 1175
end: 2597
orientation: inline

BBa_K747065
type: ComponentDefinition
persistent identity: BBa K747065
display id: BBa_K747065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 5
sequence: https://synbiohub.org/public/igem/BBa_K747065_sequence/1

AC 5
type: SequenceAnnotation
persistent identity: annotation2197022
display id: annotation2197022
version: 1
top level: BBa_K747065
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747065/annotation2197022/range2197022/1

https://synbiohub.org/public/igem/BBa_K747065/annotation2197022/range2197022/1
type: Range
persistent identity: range2197022
display id: range2197022
version: 1
direction: direction/forward
top level: BBa_K747065
start: 14
end: 211
orientation: inline

BBa_K747088
type: ComponentDefinition
persistent identity: BBa K747088
display id: BBa_K747088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 6
sequence: https://synbiohub.org/public/igem/BBa_K747088_sequence/1

GA 6
type: SequenceAnnotation
persistent identity: annotation2197064
display id: annotation2197064
version: 1
top level: BBa_K747088
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747088/annotation2197064/range2197064/1

https://synbiohub.org/public/igem/BBa_K747088/annotation2197064/range2197064/1
type: Range
persistent identity: range2197064
display id: range2197064
version: 1
direction: direction/forward
top level: BBa_K747088
start: 14
end: 211
orientation: inline

BBa_I6031
type: ComponentDefinition
persistent identity: BBa I6031
display id: BBa_I6031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0011.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0430
component: BBa_R0011
sequence annotation: BBa_E0430
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I6031_sequence/1

BBa_E0430
type: Component
persistent identity: component2221245
display id: component2221245
version: 1
top level: BBa_I6031
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_R0011
type: Component
persistent identity: component2221231
display id: component2221231
version: 1
top level: BBa_I6031
access: public
definition: lacI+pL

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2221245
display id: annotation2221245
version: 1
top level: BBa_I6031
location: https://synbiohub.org/public/igem/BBa_I6031/annotation2221245/range2221245/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_I6031/annotation2221245/range2221245/1
type: Range
persistent identity: range2221245
display id: range2221245
version: 1
direction: direction/reverse
top level: BBa_I6031
start: 64
end: 941
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221231
display id: annotation2221231
version: 1
top level: BBa_I6031
location: https://synbiohub.org/public/igem/BBa_I6031/annotation2221231/range2221231/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I6031/annotation2221231/range2221231/1
type: Range
persistent identity: range2221231
display id: range2221231
version: 1
direction: direction/forward
top level: BBa_I6031
start: 1
end: 54
orientation: inline

BBa_K747056
type: ComponentDefinition
persistent identity: BBa K747056
display id: BBa_K747056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 4
sequence: https://synbiohub.org/public/igem/BBa_K747056_sequence/1

GA 4
type: SequenceAnnotation
persistent identity: annotation2197013
display id: annotation2197013
version: 1
top level: BBa_K747056
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747056/annotation2197013/range2197013/1

https://synbiohub.org/public/igem/BBa_K747056/annotation2197013/range2197013/1
type: Range
persistent identity: range2197013
display id: range2197013
version: 1
direction: direction/forward
top level: BBa_K747056
start: 14
end: 211
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline repressor from transposon Tn10 (+LVA)
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
direction: direction/forward
top level: tetR
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
direction: direction/forward
top level: tetR
start: 661
end: 685
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
direction: direction/forward
top level: tetR
start: 4
end: 620
orientation: inline

BBa_K747095
type: ComponentDefinition
persistent identity: BBa K747095
display id: BBa_K747095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 6
sequence: https://synbiohub.org/public/igem/BBa_K747095_sequence/1

TT 6
type: SequenceAnnotation
persistent identity: annotation2197073
display id: annotation2197073
version: 1
top level: BBa_K747095
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747095/annotation2197073/range2197073/1

https://synbiohub.org/public/igem/BBa_K747095/annotation2197073/range2197073/1
type: Range
persistent identity: range2197073
display id: range2197073
version: 1
direction: direction/forward
top level: BBa_K747095
start: 14
end: 211
orientation: inline

BBa_K747080
type: ComponentDefinition
persistent identity: BBa K747080
display id: BBa_K747080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 6
sequence: https://synbiohub.org/public/igem/BBa_K747080_sequence/1

AA 6
type: SequenceAnnotation
persistent identity: annotation2197052
display id: annotation2197052
version: 1
top level: BBa_K747080
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747080/annotation2197052/range2197052/1

https://synbiohub.org/public/igem/BBa_K747080/annotation2197052/range2197052/1
type: Range
persistent identity: range2197052
display id: range2197052
version: 1
direction: direction/forward
top level: BBa_K747080
start: 14
end: 211
orientation: inline

BBa_K747012
type: ComponentDefinition
persistent identity: BBa K747012
display id: BBa_K747012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 1
sequence: https://synbiohub.org/public/igem/BBa_K747012_sequence/1

TA 1
type: SequenceAnnotation
persistent identity: annotation2195920
display id: annotation2195920
version: 1
top level: BBa_K747012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747012/annotation2195920/range2195920/1

https://synbiohub.org/public/igem/BBa_K747012/annotation2195920/range2195920/1
type: Range
persistent identity: range2195920
display id: range2195920
version: 1
direction: direction/forward
top level: BBa_K747012
start: 12
end: 209
orientation: inline

BBa_K747048
type: ComponentDefinition
persistent identity: BBa K747048
display id: BBa_K747048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 4
sequence: https://synbiohub.org/public/igem/BBa_K747048_sequence/1

AA 4
type: SequenceAnnotation
persistent identity: annotation2196273
display id: annotation2196273
version: 1
top level: BBa_K747048
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747048/annotation2196273/range2196273/1

https://synbiohub.org/public/igem/BBa_K747048/annotation2196273/range2196273/1
type: Range
persistent identity: range2196273
display id: range2196273
version: 1
direction: direction/forward
top level: BBa_K747048
start: 14
end: 211
orientation: inline

BBa_K747034
type: ComponentDefinition
persistent identity: BBa K747034
display id: BBa_K747034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 3
sequence: https://synbiohub.org/public/igem/BBa_K747034_sequence/1

AG 3
type: SequenceAnnotation
persistent identity: annotation2196210
display id: annotation2196210
version: 1
top level: BBa_K747034
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747034/annotation2196210/range2196210/1

https://synbiohub.org/public/igem/BBa_K747034/annotation2196210/range2196210/1
type: Range
persistent identity: range2196210
display id: range2196210
version: 1
direction: direction/forward
top level: BBa_K747034
start: 14
end: 211
orientation: inline

BBa_K801012
type: ComponentDefinition
persistent identity: BBa K801012
display id: BBa_K801012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 yeast terminator
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ADH1 terminator of ''S. cerevisiae''
mutable notes: -
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801012_sequence/1

ADH1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202145
display id: annotation2202145
version: 1
top level: BBa_K801012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1

https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1
type: Range
persistent identity: range2202145
display id: range2202145
version: 1
direction: direction/forward
top level: BBa_K801012
start: 1
end: 349
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: engineered cyan fluorescent protein derived from A. victoria GFP
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_K747038
type: ComponentDefinition
persistent identity: BBa K747038
display id: BBa_K747038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 3
sequence: https://synbiohub.org/public/igem/BBa_K747038_sequence/1

CG 3
type: SequenceAnnotation
persistent identity: annotation2196220
display id: annotation2196220
version: 1
top level: BBa_K747038
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747038/annotation2196220/range2196220/1

https://synbiohub.org/public/igem/BBa_K747038/annotation2196220/range2196220/1
type: Range
persistent identity: range2196220
display id: range2196220
version: 1
direction: direction/forward
top level: BBa_K747038
start: 14
end: 211
orientation: inline

BBa_K844008
type: ComponentDefinition
persistent identity: BBa K844008
display id: BBa_K844008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized) with Met (ATG) added
creator: Andrea Halling
created: 2012-10-01T11:00:00Z
modified: 2016-02-10T11:28:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1104_
m user id: 4206
owner id: 13876
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spider silk subunit optimized to use a reduced set of tRNA codons (1 or 2 codons per amino acid); contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.
mutable notes: none
mutable provenance: Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844004
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker Domain
sequence annotation: Strength Domain
sequence annotation: Elasticity Domain
sequence annotation: Beta-spiral
sequence annotation: Beta-helix
sequence annotation: Beta-sprial
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K844008_sequence/1

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212464
display id: annotation2212464
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212464/range2212464/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212464/range2212464/1
type: Range
persistent identity: range2212464
display id: range2212464
version: 1
direction: direction/forward
top level: BBa_K844008
start: 172
end: 188
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212465
display id: annotation2212465
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212465/range2212465/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212465/range2212465/1
type: Range
persistent identity: range2212465
display id: range2212465
version: 1
direction: direction/forward
top level: BBa_K844008
start: 189
end: 207
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212459
display id: annotation2212459
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212459/range2212459/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212459/range2212459/1
type: Range
persistent identity: range2212459
display id: range2212459
version: 1
direction: direction/forward
top level: BBa_K844008
start: 88
end: 171
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212456
display id: annotation2212456
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212456/range2212456/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212456/range2212456/1
type: Range
persistent identity: range2212456
display id: range2212456
version: 1
direction: direction/forward
top level: BBa_K844008
start: 49
end: 63
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212462
display id: annotation2212462
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212462/range2212462/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212462/range2212462/1
type: Range
persistent identity: range2212462
display id: range2212462
version: 1
direction: direction/forward
top level: BBa_K844008
start: 133
end: 147
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212463
display id: annotation2212463
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212463/range2212463/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212463/range2212463/1
type: Range
persistent identity: range2212463
display id: range2212463
version: 1
direction: direction/forward
top level: BBa_K844008
start: 156
end: 171
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212457
display id: annotation2212457
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212457/range2212457/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212457/range2212457/1
type: Range
persistent identity: range2212457
display id: range2212457
version: 1
direction: direction/forward
top level: BBa_K844008
start: 72
end: 87
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212458
display id: annotation2212458
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212458/range2212458/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212458/range2212458/1
type: Range
persistent identity: range2212458
display id: range2212458
version: 1
direction: direction/forward
top level: BBa_K844008
start: 88
end: 97
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212454
display id: annotation2212454
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212454/range2212454/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212454/range2212454/1
type: Range
persistent identity: range2212454
display id: range2212454
version: 1
direction: direction/forward
top level: BBa_K844008
start: 13
end: 27
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212455
display id: annotation2212455
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212455/range2212455/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212455/range2212455/1
type: Range
persistent identity: range2212455
display id: range2212455
version: 1
direction: direction/forward
top level: BBa_K844008
start: 34
end: 48
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212453
display id: annotation2212453
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212453/range2212453/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212453/range2212453/1
type: Range
persistent identity: range2212453
display id: range2212453
version: 1
direction: direction/forward
top level: BBa_K844008
start: 4
end: 87
orientation: inline

Beta-sprial
type: SequenceAnnotation
persistent identity: annotation2212461
display id: annotation2212461
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212461/range2212461/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212461/range2212461/1
type: Range
persistent identity: range2212461
display id: range2212461
version: 1
direction: direction/forward
top level: BBa_K844008
start: 117
end: 132
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212460
display id: annotation2212460
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212460/range2212460/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212460/range2212460/1
type: Range
persistent identity: range2212460
display id: range2212460
version: 1
direction: direction/forward
top level: BBa_K844008
start: 98
end: 113
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2212451
display id: annotation2212451
version: 1
top level: BBa_K844008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212451/range2212451/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212451/range2212451/1
type: Range
persistent identity: range2212451
display id: range2212451
version: 1
direction: direction/forward
top level: BBa_K844008
start: 1
end: 3
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212452
display id: annotation2212452
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212452/range2212452/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212452/range2212452/1
type: Range
persistent identity: range2212452
display id: range2212452
version: 1
direction: direction/forward
top level: BBa_K844008
start: 4
end: 12
orientation: inline

BBa_I732018
type: ComponentDefinition
persistent identity: BBa I732018
display id: BBa_I732018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ alpha fragment (RBS+ TERM-)
creator: Zhan Jian
created: 2007-07-13T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: None
mutable notes: None
mutable provenance: I732006 inserted into pSB1A2-B0034
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_I732006
sequence annotation: BBa_I732006
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732018_sequence/1

BBa_B0034
type: Component
persistent identity: component1938196
display id: component1938196
version: 1
top level: BBa_I732018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732006
type: Component
persistent identity: component1938200
display id: component1938200
version: 1
top level: BBa_I732018
access: public
definition: https://synbiohub.org/public/igem/BBa_I732006/1

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1938200
display id: annotation1938200
version: 1
top level: BBa_I732018
location: https://synbiohub.org/public/igem/BBa_I732018/annotation1938200/range1938200/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732018/annotation1938200/range1938200/1
type: Range
persistent identity: range1938200
display id: range1938200
version: 1
direction: direction/reverse
top level: BBa_I732018
start: 19
end: 252
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1938196
display id: annotation1938196
version: 1
top level: BBa_I732018
location: https://synbiohub.org/public/igem/BBa_I732018/annotation1938196/range1938196/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732018/annotation1938196/range1938196/1
type: Range
persistent identity: range1938196
display id: range1938196
version: 1
direction: direction/reverse
top level: BBa_I732018
start: 1
end: 12
orientation: inline

BBa_J202010
type: ComponentDefinition
persistent identity: BBa J202010
display id: BBa_J202010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#11
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -58 relative to the araC transcriptional start site was changed from A to C.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI2
sequence annotation: araI1
sequence annotation: Transcription Start
sequence annotation: Mutation
sequence: https://synbiohub.org/public/igem/BBa_J202010_sequence/1

araI2
type: SequenceAnnotation
persistent identity: annotation2178460
display id: annotation2178460
version: 1
top level: BBa_J202010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202010/annotation2178460/range2178460/1

https://synbiohub.org/public/igem/BBa_J202010/annotation2178460/range2178460/1
type: Range
persistent identity: range2178460
display id: range2178460
version: 1
direction: direction/forward
top level: BBa_J202010
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178459
display id: annotation2178459
version: 1
top level: BBa_J202010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202010/annotation2178459/range2178459/1

https://synbiohub.org/public/igem/BBa_J202010/annotation2178459/range2178459/1
type: Range
persistent identity: range2178459
display id: range2178459
version: 1
direction: direction/forward
top level: BBa_J202010
start: 40
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178458
display id: annotation2178458
version: 1
top level: BBa_J202010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202010/annotation2178458/range2178458/1

https://synbiohub.org/public/igem/BBa_J202010/annotation2178458/range2178458/1
type: Range
persistent identity: range2178458
display id: range2178458
version: 1
direction: direction/forward
top level: BBa_J202010
start: 112
end: 112
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178457
display id: annotation2178457
version: 1
top level: BBa_J202010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202010/annotation2178457/range2178457/1

https://synbiohub.org/public/igem/BBa_J202010/annotation2178457/range2178457/1
type: Range
persistent identity: range2178457
display id: range2178457
version: 1
direction: direction/forward
top level: BBa_J202010
start: 54
end: 54
orientation: inline

fhlA-E363
type: ComponentDefinition
persistent identity: BBa K778002
display id: BBa_K778002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fhlA-E363G (engineered trascriptional activator fhlA with a mutation)
creator: Yuka Yamazaki
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: fhlA363
mutable notes: E363G
mutable provenance: BBa_K778001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FhlA-E363G
sequence annotation: A to G (different from E. coli K-12 MG1655)
sequence annotation: stop
sequence annotation: E363G mutation
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K778002_sequence/1

FhlA-E363G
type: SequenceAnnotation
persistent identity: annotation2201467
display id: annotation2201467
version: 1
top level: fhlA-E363
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2201467/range2201467/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2201467/range2201467/1
type: Range
persistent identity: range2201467
display id: range2201467
version: 1
direction: direction/forward
top level: fhlA-E363
start: 1
end: 2079
orientation: inline

A to G (different from E. coli K-12 MG1655)
type: SequenceAnnotation
persistent identity: annotation2195764
display id: annotation2195764
version: 1
top level: fhlA-E363
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2195764/range2195764/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2195764/range2195764/1
type: Range
persistent identity: range2195764
display id: range2195764
version: 1
direction: direction/forward
top level: fhlA-E363
start: 1906
end: 1906
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2194870
display id: annotation2194870
version: 1
top level: fhlA-E363
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194870/range2194870/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2194870/range2194870/1
type: Range
persistent identity: range2194870
display id: range2194870
version: 1
direction: direction/forward
top level: fhlA-E363
start: 2077
end: 2079
orientation: inline

E363G mutation
type: SequenceAnnotation
persistent identity: annotation2194869
display id: annotation2194869
version: 1
top level: fhlA-E363
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194869/range2194869/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2194869/range2194869/1
type: Range
persistent identity: range2194869
display id: range2194869
version: 1
direction: direction/forward
top level: fhlA-E363
start: 1088
end: 1088
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2194868
display id: annotation2194868
version: 1
top level: fhlA-E363
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194868/range2194868/1

https://synbiohub.org/public/igem/BBa_K778002/annotation2194868/range2194868/1
type: Range
persistent identity: range2194868
display id: range2194868
version: 1
direction: direction/forward
top level: fhlA-E363
start: 1
end: 1
orientation: inline

RO
type: ComponentDefinition
persistent identity: BBa K838001
display id: BBa_K838001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LovTAP readout
creator: Nicolas Gobet
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1097_
m user id: 0
owner id: 12458
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Expresses dsRed
mutable notes: Has 2 SpeI and 1 EcoRI site init
mutable provenance: Nicolas Gobet
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K838001_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa I13503
display id: BBa_I13503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E1010
component: BBa_B0032
sequence annotation: BBa_E1010
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I13503_sequence/1

BBa_E1010
type: Component
persistent identity: component1505061
display id: component1505061
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0032
type: Component
persistent identity: component1505053
display id: component1505053
version: 1
top level: mRFP1
access: public
definition: BBa_B0032

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505061
display id: annotation1505061
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13503/annotation1505061/range1505061/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13503/annotation1505061/range1505061/1
type: Range
persistent identity: range1505061
display id: range1505061
version: 1
direction: direction/forward
top level: mRFP1
start: 20
end: 700
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1505053
display id: annotation1505053
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13503/annotation1505053/range1505053/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13503/annotation1505053/range1505053/1
type: Range
persistent identity: range1505053
display id: range1505053
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 13
orientation: inline

BBa_K598014
type: ComponentDefinition
persistent identity: BBa K598014
display id: BBa_K598014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+HH+CdIntron+E0040+b0015
creator: Xiaowei YAN, Tong MU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
mutable notes: The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
mutable provenance: The intron derives from the genome of Cd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS
sequence annotation: eGFP
sequence annotation: pBAD
sequence annotation: BBa_B0015
sequence annotation: group I intron
sequence annotation: HH
sequence: https://synbiohub.org/public/igem/BBa_K598014_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2153089
display id: annotation2153089
version: 1
top level: BBa_K598014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153089/range2153089/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153089/range2153089/1
type: Range
persistent identity: range2153089
display id: range2153089
version: 1
direction: direction/forward
top level: BBa_K598014
start: 758
end: 758
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153088
display id: annotation2153088
version: 1
top level: BBa_K598014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153088/range2153088/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153088/range2153088/1
type: Range
persistent identity: range2153088
display id: range2153088
version: 1
direction: direction/forward
top level: BBa_K598014
start: 187
end: 191
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2153086
display id: annotation2153086
version: 1
top level: BBa_K598014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153086/range2153086/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153086/range2153086/1
type: Range
persistent identity: range2153086
display id: range2153086
version: 1
direction: direction/forward
top level: BBa_K598014
start: 765
end: 1484
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2153081
display id: annotation2153081
version: 1
top level: BBa_K598014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153081/range2153081/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153081/range2153081/1
type: Range
persistent identity: range2153081
display id: range2153081
version: 1
direction: direction/forward
top level: BBa_K598014
start: 1
end: 130
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153087
display id: annotation2153087
version: 1
top level: BBa_K598014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153087/range2153087/1

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type: Range
persistent identity: range2153087
display id: range2153087
version: 1
direction: direction/forward
top level: BBa_K598014
start: 1493
end: 1621
orientation: inline

group I intron
type: SequenceAnnotation
persistent identity: annotation2153083
display id: annotation2153083
version: 1
top level: BBa_K598014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153083/range2153083/1

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type: Range
persistent identity: range2153083
display id: range2153083
version: 1
direction: direction/forward
top level: BBa_K598014
start: 186
end: 764
orientation: inline

HH
type: SequenceAnnotation
persistent identity: annotation2153082
display id: annotation2153082
version: 1
top level: BBa_K598014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153082/range2153082/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153082/range2153082/1
type: Range
persistent identity: range2153082
display id: range2153082
version: 1
direction: direction/forward
top level: BBa_K598014
start: 139
end: 185
orientation: inline

BBa_I732903
type: ComponentDefinition
persistent identity: BBa I732903
display id: BBa_I732903
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011 I732020
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_I732006
component: BBa_B0012
component: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I732903_sequence/1

BBa_B0010
type: Component
persistent identity: component1948581
display id: component1948581
version: 1
top level: BBa_I732903
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1948576
display id: component1948576
version: 1
top level: BBa_I732903
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732006
type: Component
persistent identity: component1948580
display id: component1948580
version: 1
top level: BBa_I732903
access: public
definition: https://synbiohub.org/public/igem/BBa_I732006/1

BBa_B0012
type: Component
persistent identity: component1948583
display id: component1948583
version: 1
top level: BBa_I732903
access: public
definition: BBa_B0012

BBa_R0011
type: Component
persistent identity: component1948570
display id: component1948570
version: 1
top level: BBa_I732903
access: public
definition: lacI+pL

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948581
display id: annotation1948581
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948581/range1948581/1
component: BBa_B0010

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type: Range
persistent identity: range1948581
display id: range1948581
version: 1
direction: direction/forward
top level: BBa_I732903
start: 324
end: 403
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948583
display id: annotation1948583
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948583/range1948583/1
component: BBa_B0012

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type: Range
persistent identity: range1948583
display id: range1948583
version: 1
direction: direction/forward
top level: BBa_I732903
start: 412
end: 452
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948576
display id: annotation1948576
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948576/range1948576/1
component: BBa_B0034

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type: Range
persistent identity: range1948576
display id: range1948576
version: 1
direction: direction/reverse
top level: BBa_I732903
start: 64
end: 75
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948580
display id: annotation1948580
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948580/range1948580/1
component: BBa_I732006

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type: Range
persistent identity: range1948580
display id: range1948580
version: 1
direction: direction/reverse
top level: BBa_I732903
start: 82
end: 315
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1948570
display id: annotation1948570
version: 1
top level: BBa_I732903
location: https://synbiohub.org/public/igem/BBa_I732903/annotation1948570/range1948570/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I732903/annotation1948570/range1948570/1
type: Range
persistent identity: range1948570
display id: range1948570
version: 1
direction: direction/forward
top level: BBa_I732903
start: 1
end: 54
orientation: inline

TD0.6
type: ComponentDefinition
persistent identity: BBa K750010
display id: BBa_K750010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP
creator: Sifan Wang, Ruosang Qiu, Shuqin Hu,Zhao Ma,Yunxin Long
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains part5 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
mutable notes: editing...
mutable provenance: We built this part by biobricks from the DNA distribution kit plates 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K750002
component: BBa_K750007
sequence annotation: BBa_K750002
sequence annotation: BBa_K750007
sequence: https://synbiohub.org/public/igem/BBa_K750010_sequence/1

BBa_K750002
type: Component
persistent identity: component2312665
display id: component2312665
version: 1
top level: TD0.6
access: public
definition: https://synbiohub.org/public/igem/BBa_K750002/1

BBa_K750007
type: Component
persistent identity: component2312702
display id: component2312702
version: 1
top level: TD0.6
access: public
definition: https://synbiohub.org/public/igem/BBa_K750007/1

BBa_K750002
type: SequenceAnnotation
persistent identity: annotation2312665
display id: annotation2312665
version: 1
top level: TD0.6
location: https://synbiohub.org/public/igem/BBa_K750010/annotation2312665/range2312665/1
component: BBa_K750002

https://synbiohub.org/public/igem/BBa_K750010/annotation2312665/range2312665/1
type: Range
persistent identity: range2312665
display id: range2312665
version: 1
direction: direction/reverse
top level: TD0.6
start: 1
end: 939
orientation: inline

BBa_K750007
type: SequenceAnnotation
persistent identity: annotation2312702
display id: annotation2312702
version: 1
top level: TD0.6
location: https://synbiohub.org/public/igem/BBa_K750010/annotation2312702/range2312702/1
component: BBa_K750007

https://synbiohub.org/public/igem/BBa_K750010/annotation2312702/range2312702/1
type: Range
persistent identity: range2312702
display id: range2312702
version: 1
direction: direction/reverse
top level: TD0.6
start: 948
end: 2967
orientation: inline

BBa_K519030
type: ComponentDefinition
persistent identity: BBa K519030
display id: BBa_K519030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Photocontrol device
creator: Chifuku Mita
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _684_
m user id: 0
owner id: 9626
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I15008
component: BBa_I15009
component: BBa_B0015
component: BBa_I15010
sequence annotation: BBa_I15008
sequence annotation: BBa_B0034
sequence annotation: BBa_I15010
sequence annotation: BBa_B0015
sequence annotation: BBa_I15009
sequence: https://synbiohub.org/public/igem/BBa_K519030_sequence/1

BBa_B0034
type: Component
persistent identity: component2273716
display id: component2273716
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15008
type: Component
persistent identity: component2273714
display id: component2273714
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_I15009
type: Component
persistent identity: component2273719
display id: component2273719
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_I15009/1

BBa_B0034
type: Component
persistent identity: component2273712
display id: component2273712
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2273721
display id: component2273721
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2273731
display id: component2273731
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I15010
type: Component
persistent identity: component2273724
display id: component2273724
version: 1
top level: BBa_K519030
access: public
definition: https://synbiohub.org/public/igem/BBa_I15010/1

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2273714
display id: annotation2273714
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273714/range2273714/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K519030/annotation2273714/range2273714/1
type: Range
persistent identity: range2273714
display id: range2273714
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 19
end: 769
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273721
display id: annotation2273721
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273721/range2273721/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K519030/annotation2273721/range2273721/1
type: Range
persistent identity: range2273721
display id: range2273721
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 1579
end: 1590
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273716
display id: annotation2273716
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273716/range2273716/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K519030/annotation2273716/range2273716/1
type: Range
persistent identity: range2273716
display id: range2273716
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 778
end: 789
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273712
display id: annotation2273712
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273712/range2273712/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K519030/annotation2273712/range2273712/1
type: Range
persistent identity: range2273712
display id: range2273712
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 1
end: 12
orientation: inline

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation2273724
display id: annotation2273724
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273724/range2273724/1
component: BBa_I15010

https://synbiohub.org/public/igem/BBa_K519030/annotation2273724/range2273724/1
type: Range
persistent identity: range2273724
display id: range2273724
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 1597
end: 3859
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273731
display id: annotation2273731
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273731/range2273731/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K519030/annotation2273731/range2273731/1
type: Range
persistent identity: range2273731
display id: range2273731
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 3868
end: 3996
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2273719
display id: annotation2273719
version: 1
top level: BBa_K519030
location: https://synbiohub.org/public/igem/BBa_K519030/annotation2273719/range2273719/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K519030/annotation2273719/range2273719/1
type: Range
persistent identity: range2273719
display id: range2273719
version: 1
direction: direction/reverse
top level: BBa_K519030
start: 796
end: 1570
orientation: inline

BBa_J202013
type: ComponentDefinition
persistent identity: BBa J202013
display id: BBa_J202013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#14
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -69 relative to the araC transcriptional start site was changed from C to A.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Start
sequence annotation: araI2
sequence annotation: Mutation
sequence annotation: araI1
sequence: https://synbiohub.org/public/igem/BBa_J202013_sequence/1

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178470
display id: annotation2178470
version: 1
top level: BBa_J202013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202013/annotation2178470/range2178470/1

https://synbiohub.org/public/igem/BBa_J202013/annotation2178470/range2178470/1
type: Range
persistent identity: range2178470
display id: range2178470
version: 1
direction: direction/forward
top level: BBa_J202013
start: 112
end: 112
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178472
display id: annotation2178472
version: 1
top level: BBa_J202013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202013/annotation2178472/range2178472/1

https://synbiohub.org/public/igem/BBa_J202013/annotation2178472/range2178472/1
type: Range
persistent identity: range2178472
display id: range2178472
version: 1
direction: direction/forward
top level: BBa_J202013
start: 61
end: 77
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178469
display id: annotation2178469
version: 1
top level: BBa_J202013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202013/annotation2178469/range2178469/1

https://synbiohub.org/public/igem/BBa_J202013/annotation2178469/range2178469/1
type: Range
persistent identity: range2178469
display id: range2178469
version: 1
direction: direction/forward
top level: BBa_J202013
start: 43
end: 43
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178471
display id: annotation2178471
version: 1
top level: BBa_J202013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202013/annotation2178471/range2178471/1

https://synbiohub.org/public/igem/BBa_J202013/annotation2178471/range2178471/1
type: Range
persistent identity: range2178471
display id: range2178471
version: 1
direction: direction/forward
top level: BBa_J202013
start: 40
end: 56
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.3 (medium) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
direction: direction/forward
top level: BBa_B0032
start: 7
end: 10
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

BBa_I6056
type: ComponentDefinition
persistent identity: BBa I6056
display id: BBa_I6056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1062.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0030
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_R1062
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_R1062
sequence: https://synbiohub.org/public/igem/BBa_I6056_sequence/1

BBa_E0030
type: Component
persistent identity: component948809
display id: component948809
version: 1
top level: BBa_I6056
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component948807
display id: component948807
version: 1
top level: BBa_I6056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component948814
display id: component948814
version: 1
top level: BBa_I6056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component948824
display id: component948824
version: 1
top level: BBa_I6056
access: public
definition: BBa_B0012

BBa_R1062
type: Component
persistent identity: component948799
display id: component948799
version: 1
top level: BBa_I6056
access: public
definition: lux pR

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948807
display id: annotation948807
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948807/range948807/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6056/annotation948807/range948807/1
type: Range
persistent identity: range948807
display id: range948807
version: 1
direction: direction/forward
top level: BBa_I6056
start: 65
end: 76
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948814
display id: annotation948814
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948814/range948814/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6056/annotation948814/range948814/1
type: Range
persistent identity: range948814
display id: range948814
version: 1
direction: direction/forward
top level: BBa_I6056
start: 814
end: 893
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948824
display id: annotation948824
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948824/range948824/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6056/annotation948824/range948824/1
type: Range
persistent identity: range948824
display id: range948824
version: 1
direction: direction/forward
top level: BBa_I6056
start: 902
end: 942
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948809
display id: annotation948809
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948809/range948809/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6056/annotation948809/range948809/1
type: Range
persistent identity: range948809
display id: range948809
version: 1
direction: direction/forward
top level: BBa_I6056
start: 83
end: 805
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation948799
display id: annotation948799
version: 1
top level: BBa_I6056
location: https://synbiohub.org/public/igem/BBa_I6056/annotation948799/range948799/1
component: BBa_R1062

https://synbiohub.org/public/igem/BBa_I6056/annotation948799/range948799/1
type: Range
persistent identity: range948799
display id: range948799
version: 1
direction: direction/forward
top level: BBa_I6056
start: 1
end: 56
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: SsrA
sequence annotation: A (Q->L)
sequence annotation: BBa_E0032
sequence annotation: YFP (LVA)
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
direction: direction/forward
top level: YFP
start: 718
end: 756
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
direction: direction/forward
top level: YFP
start: 242
end: 242
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
direction: direction/forward
top level: YFP
start: 757
end: 762
orientation: inline

BBa_I6084
type: ComponentDefinition
persistent identity: BBa I6084
display id: BBa_I6084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1053.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0032
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_R1053
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R1053
sequence: https://synbiohub.org/public/igem/BBa_I6084_sequence/1

BBa_E0032
type: Component
persistent identity: component949564
display id: component949564
version: 1
top level: BBa_I6084
access: public
definition: YFP

BBa_B0034
type: Component
persistent identity: component949552
display id: component949552
version: 1
top level: BBa_I6084
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component949572
display id: component949572
version: 1
top level: BBa_I6084
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949582
display id: component949582
version: 1
top level: BBa_I6084
access: public
definition: BBa_B0012

BBa_R1053
type: Component
persistent identity: component949542
display id: component949542
version: 1
top level: BBa_I6084
access: public
definition: https://synbiohub.org/public/igem/BBa_R1053/1

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949564
display id: annotation949564
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949564/range949564/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6084/annotation949564/range949564/1
type: Range
persistent identity: range949564
display id: range949564
version: 1
direction: direction/forward
top level: BBa_I6084
start: 82
end: 843
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949582
display id: annotation949582
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949582/range949582/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6084/annotation949582/range949582/1
type: Range
persistent identity: range949582
display id: range949582
version: 1
direction: direction/forward
top level: BBa_I6084
start: 940
end: 980
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949572
display id: annotation949572
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949572/range949572/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6084/annotation949572/range949572/1
type: Range
persistent identity: range949572
display id: range949572
version: 1
direction: direction/forward
top level: BBa_I6084
start: 852
end: 931
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949552
display id: annotation949552
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949552/range949552/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6084/annotation949552/range949552/1
type: Range
persistent identity: range949552
display id: range949552
version: 1
direction: direction/forward
top level: BBa_I6084
start: 64
end: 75
orientation: inline

BBa_R1053
type: SequenceAnnotation
persistent identity: annotation949542
display id: annotation949542
version: 1
top level: BBa_I6084
location: https://synbiohub.org/public/igem/BBa_I6084/annotation949542/range949542/1
component: BBa_R1053

https://synbiohub.org/public/igem/BBa_I6084/annotation949542/range949542/1
type: Range
persistent identity: range949542
display id: range949542
version: 1
direction: direction/forward
top level: BBa_I6084
start: 1
end: 55
orientation: inline

BBa_I7108
type: ComponentDefinition
persistent identity: BBa I7108
display id: BBa_I7108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0053.E0840
creator: Jennifer Braff
created: 2005-07-27T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0053.E0840 (S_N; strong rbs; low PoPS)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_B0030
component: BBa_R0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0053
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I7108_sequence/1

BBa_E0040
type: Component
persistent identity: component1596676
display id: component1596676
version: 1
top level: BBa_I7108
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1596681
display id: component1596681
version: 1
top level: BBa_I7108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1596691
display id: component1596691
version: 1
top level: BBa_I7108
access: public
definition: BBa_B0012

BBa_B0030
type: Component
persistent identity: component1596673
display id: component1596673
version: 1
top level: BBa_I7108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0053
type: Component
persistent identity: component1596660
display id: component1596660
version: 1
top level: BBa_I7108
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1596681
display id: annotation1596681
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596681/range1596681/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I7108/annotation1596681/range1596681/1
type: Range
persistent identity: range1596681
display id: range1596681
version: 1
direction: direction/forward
top level: BBa_I7108
start: 812
end: 891
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1596673
display id: annotation1596673
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596673/range1596673/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I7108/annotation1596673/range1596673/1
type: Range
persistent identity: range1596673
display id: range1596673
version: 1
direction: direction/forward
top level: BBa_I7108
start: 63
end: 77
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1596691
display id: annotation1596691
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596691/range1596691/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I7108/annotation1596691/range1596691/1
type: Range
persistent identity: range1596691
display id: range1596691
version: 1
direction: direction/forward
top level: BBa_I7108
start: 900
end: 940
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1596660
display id: annotation1596660
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596660/range1596660/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I7108/annotation1596660/range1596660/1
type: Range
persistent identity: range1596660
display id: range1596660
version: 1
direction: direction/forward
top level: BBa_I7108
start: 1
end: 54
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1596676
display id: annotation1596676
version: 1
top level: BBa_I7108
location: https://synbiohub.org/public/igem/BBa_I7108/annotation1596676/range1596676/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I7108/annotation1596676/range1596676/1
type: Range
persistent identity: range1596676
display id: range1596676
version: 1
direction: direction/forward
top level: BBa_I7108
start: 84
end: 803
orientation: inline

BBa_I6207
type: ComponentDefinition
persistent identity: BBa I6207
display id: BBa_I6207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0079.E0432
creator: Jennifer Braff
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0079
component: BBa_B0012
component: BBa_B0010
component: BBa_E0032
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0032
sequence annotation: BBa_R0079
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6207_sequence/1

BBa_R0079
type: Component
persistent identity: component950570
display id: component950570
version: 1
top level: BBa_I6207
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0012
type: Component
persistent identity: component950605
display id: component950605
version: 1
top level: BBa_I6207
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component950595
display id: component950595
version: 1
top level: BBa_I6207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0032
type: Component
persistent identity: component950587
display id: component950587
version: 1
top level: BBa_I6207
access: public
definition: YFP

BBa_B0034
type: Component
persistent identity: component950575
display id: component950575
version: 1
top level: BBa_I6207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation950595
display id: annotation950595
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950595/range950595/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6207/annotation950595/range950595/1
type: Range
persistent identity: range950595
display id: range950595
version: 1
direction: direction/forward
top level: BBa_I6207
start: 954
end: 1033
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation950587
display id: annotation950587
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950587/range950587/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6207/annotation950587/range950587/1
type: Range
persistent identity: range950587
display id: range950587
version: 1
direction: direction/forward
top level: BBa_I6207
start: 184
end: 945
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation950570
display id: annotation950570
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950570/range950570/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_I6207/annotation950570/range950570/1
type: Range
persistent identity: range950570
display id: range950570
version: 1
direction: direction/forward
top level: BBa_I6207
start: 1
end: 157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation950575
display id: annotation950575
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950575/range950575/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6207/annotation950575/range950575/1
type: Range
persistent identity: range950575
display id: range950575
version: 1
direction: direction/forward
top level: BBa_I6207
start: 166
end: 177
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation950605
display id: annotation950605
version: 1
top level: BBa_I6207
location: https://synbiohub.org/public/igem/BBa_I6207/annotation950605/range950605/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6207/annotation950605/range950605/1
type: Range
persistent identity: range950605
display id: range950605
version: 1
direction: direction/forward
top level: BBa_I6207
start: 1042
end: 1082
orientation: inline

GFP-AID
type: ComponentDefinition
persistent identity: BBa K812010
display id: BBa_K812010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)
creator: Cyrille Pauthenier
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1069_
m user id: 0
owner id: 8998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken. The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
mutable notes: This part has been biobricked using PCR method and cloned in pSB1C3
mutable provenance: This part have been extracted by PCR from the plasmid pNHK60 obtained from the riken registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: NLS2
sequence annotation: AID degradation tag
sequence annotation: GFP
sequence annotation: Kozac
sequence annotation: unfolded linker
sequence annotation: NLS1
sequence: https://synbiohub.org/public/igem/BBa_K812010_sequence/1

NLS2
type: SequenceAnnotation
persistent identity: annotation2184374
display id: annotation2184374
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184374/range2184374/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184374/range2184374/1
type: Range
persistent identity: range2184374
display id: range2184374
version: 1
direction: direction/forward
top level: GFP-AID
start: 1480
end: 1500
orientation: inline

AID degradation tag
type: SequenceAnnotation
persistent identity: annotation2184372
display id: annotation2184372
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184372/range2184372/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184372/range2184372/1
type: Range
persistent identity: range2184372
display id: range2184372
version: 1
direction: direction/forward
top level: GFP-AID
start: 757
end: 1443
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2184370
display id: annotation2184370
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184370/range2184370/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184370/range2184370/1
type: Range
persistent identity: range2184370
display id: range2184370
version: 1
direction: direction/forward
top level: GFP-AID
start: 7
end: 723
orientation: inline

Kozac
type: SequenceAnnotation
persistent identity: annotation2184369
display id: annotation2184369
version: 1
top level: GFP-AID
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184369/range2184369/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184369/range2184369/1
type: Range
persistent identity: range2184369
display id: range2184369
version: 1
direction: direction/forward
top level: GFP-AID
start: 1
end: 6
orientation: inline

unfolded linker
type: SequenceAnnotation
persistent identity: annotation2184371
display id: annotation2184371
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184371/range2184371/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184371/range2184371/1
type: Range
persistent identity: range2184371
display id: range2184371
version: 1
direction: direction/forward
top level: GFP-AID
start: 724
end: 756
orientation: inline

NLS1
type: SequenceAnnotation
persistent identity: annotation2184373
display id: annotation2184373
version: 1
top level: GFP-AID
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812010/annotation2184373/range2184373/1

https://synbiohub.org/public/igem/BBa_K812010/annotation2184373/range2184373/1
type: Range
persistent identity: range2184373
display id: range2184373
version: 1
direction: direction/forward
top level: GFP-AID
start: 1459
end: 1479
orientation: inline

BBa_K731255
type: ComponentDefinition
persistent identity: BBa K731255
display id: BBa_K731255
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible araC-pBAD promoter with GFPmut3b, weak RBS
creator: Daniele Rossetto
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200) when using a weak RBS. The part is composed of part BBa_K731200 and BBa_E0240
mutable provenance: araC-pBAD from E. coli genome, GFPmut3b is from the registry (part BBa_E0840)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K731201
component: BBa_E0840
sequence annotation: BBa_K731201
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K731255_sequence/1

BBa_K731201
type: Component
persistent identity: component2331139
display id: component2331139
version: 1
top level: BBa_K731255
access: public
definition: https://synbiohub.org/public/igem/BBa_K731201/1

BBa_E0840
type: Component
persistent identity: component2331150
display id: component2331150
version: 1
top level: BBa_K731255
access: public
definition: GFP genera

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2331139
display id: annotation2331139
version: 1
top level: BBa_K731255
location: https://synbiohub.org/public/igem/BBa_K731255/annotation2331139/range2331139/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K731255/annotation2331139/range2331139/1
type: Range
persistent identity: range2331139
display id: range2331139
version: 1
direction: direction/reverse
top level: BBa_K731255
start: 1
end: 1203
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2331150
display id: annotation2331150
version: 1
top level: BBa_K731255
location: https://synbiohub.org/public/igem/BBa_K731255/annotation2331150/range2331150/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K731255/annotation2331150/range2331150/1
type: Range
persistent identity: range2331150
display id: range2331150
version: 1
direction: direction/reverse
top level: BBa_K731255
start: 1212
end: 2089
orientation: inline

BBa_J202004
type: ComponentDefinition
persistent identity: BBa J202004
display id: BBa_J202004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#5
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -71 relative to the araC transcriptional start site was changed from A to G.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: araI2
sequence annotation: araI1
sequence annotation: Transcription Start
sequence: https://synbiohub.org/public/igem/BBa_J202004_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2178435
display id: annotation2178435
version: 1
top level: BBa_J202004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202004/annotation2178435/range2178435/1

https://synbiohub.org/public/igem/BBa_J202004/annotation2178435/range2178435/1
type: Range
persistent identity: range2178435
display id: range2178435
version: 1
direction: direction/forward
top level: BBa_J202004
start: 41
end: 41
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178433
display id: annotation2178433
version: 1
top level: BBa_J202004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202004/annotation2178433/range2178433/1

https://synbiohub.org/public/igem/BBa_J202004/annotation2178433/range2178433/1
type: Range
persistent identity: range2178433
display id: range2178433
version: 1
direction: direction/forward
top level: BBa_J202004
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178432
display id: annotation2178432
version: 1
top level: BBa_J202004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202004/annotation2178432/range2178432/1

https://synbiohub.org/public/igem/BBa_J202004/annotation2178432/range2178432/1
type: Range
persistent identity: range2178432
display id: range2178432
version: 1
direction: direction/forward
top level: BBa_J202004
start: 40
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178434
display id: annotation2178434
version: 1
top level: BBa_J202004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202004/annotation2178434/range2178434/1

https://synbiohub.org/public/igem/BBa_J202004/annotation2178434/range2178434/1
type: Range
persistent identity: range2178434
display id: range2178434
version: 1
direction: direction/forward
top level: BBa_J202004
start: 112
end: 112
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2 cI
sequence annotation: lux p(R) start
sequence annotation: -10
sequence annotation: BBa_R0065
sequence annotation: -35
sequence annotation: OR1 cI
sequence annotation: Lux Box
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.2 (weak) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
direction: direction/forward
top level: BBa_B0031
start: 7
end: 10
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI 434
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
direction: direction/forward
top level: cI 434
start: 631
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
direction: direction/forward
top level: cI 434
start: 670
end: 694
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

BBa_I6105
type: ComponentDefinition
persistent identity: BBa I6105
display id: BBa_I6105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0077.E0430
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0077
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_R0077
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6105_sequence/1

BBa_R0077
type: Component
persistent identity: component949853
display id: component949853
version: 1
top level: BBa_I6105
access: public
definition: https://synbiohub.org/public/igem/BBa_R0077/1

BBa_B0012
type: Component
persistent identity: component949875
display id: component949875
version: 1
top level: BBa_I6105
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component949858
display id: component949858
version: 1
top level: BBa_I6105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component949865
display id: component949865
version: 1
top level: BBa_I6105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component949860
display id: component949860
version: 1
top level: BBa_I6105
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation949853
display id: annotation949853
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949853/range949853/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_I6105/annotation949853/range949853/1
type: Range
persistent identity: range949853
display id: range949853
version: 1
direction: direction/forward
top level: BBa_I6105
start: 1
end: 231
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949860
display id: annotation949860
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949860/range949860/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6105/annotation949860/range949860/1
type: Range
persistent identity: range949860
display id: range949860
version: 1
direction: direction/forward
top level: BBa_I6105
start: 258
end: 980
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949875
display id: annotation949875
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949875/range949875/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6105/annotation949875/range949875/1
type: Range
persistent identity: range949875
display id: range949875
version: 1
direction: direction/forward
top level: BBa_I6105
start: 1077
end: 1117
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949858
display id: annotation949858
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949858/range949858/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6105/annotation949858/range949858/1
type: Range
persistent identity: range949858
display id: range949858
version: 1
direction: direction/forward
top level: BBa_I6105
start: 240
end: 251
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949865
display id: annotation949865
version: 1
top level: BBa_I6105
location: https://synbiohub.org/public/igem/BBa_I6105/annotation949865/range949865/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6105/annotation949865/range949865/1
type: Range
persistent identity: range949865
display id: range949865
version: 1
direction: direction/forward
top level: BBa_I6105
start: 989
end: 1068
orientation: inline

BBa_K876057
type: ComponentDefinition
persistent identity: BBa K876057
display id: BBa_K876057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTetR-lasR-Term-Term
creator: Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pTetR-lasR-TT
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0079
component: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_C0079
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K876057_sequence/1

BBa_B0015
type: Component
persistent identity: component2251293
display id: component2251293
version: 1
top level: BBa_K876057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0079
type: Component
persistent identity: component2251286
display id: component2251286
version: 1
top level: BBa_K876057
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_R0040
type: Component
persistent identity: component2251277
display id: component2251277
version: 1
top level: BBa_K876057
access: public
definition: p(tetR)

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251293
display id: annotation2251293
version: 1
top level: BBa_K876057
location: https://synbiohub.org/public/igem/BBa_K876057/annotation2251293/range2251293/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876057/annotation2251293/range2251293/1
type: Range
persistent identity: range2251293
display id: range2251293
version: 1
direction: direction/reverse
top level: BBa_K876057
start: 850
end: 978
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2251286
display id: annotation2251286
version: 1
top level: BBa_K876057
location: https://synbiohub.org/public/igem/BBa_K876057/annotation2251286/range2251286/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K876057/annotation2251286/range2251286/1
type: Range
persistent identity: range2251286
display id: range2251286
version: 1
direction: direction/reverse
top level: BBa_K876057
start: 61
end: 841
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251277
display id: annotation2251277
version: 1
top level: BBa_K876057
location: https://synbiohub.org/public/igem/BBa_K876057/annotation2251277/range2251277/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876057/annotation2251277/range2251277/1
type: Range
persistent identity: range2251277
display id: range2251277
version: 1
direction: direction/forward
top level: BBa_K876057
start: 1
end: 54
orientation: inline

BBa_I13541
type: ComponentDefinition
persistent identity: BBa I13541
display id: BBa_I13541
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate in two promoter screening plasmid
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_E0040
component: BBa_B0034
component: BBa_B0010
component: BBa_I13453
component: BBa_B0012
sequence annotation: BBa_I13453
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13541_sequence/1

BBa_E0040
type: Component
persistent identity: component1663876
display id: component1663876
version: 1
top level: BBa_I13541
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component1663874
display id: component1663874
version: 1
top level: BBa_I13541
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1663881
display id: component1663881
version: 1
top level: BBa_I13541
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13453
type: Component
persistent identity: component1663869
display id: component1663869
version: 1
top level: BBa_I13541
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0012
type: Component
persistent identity: component1663891
display id: component1663891
version: 1
top level: BBa_I13541
access: public
definition: BBa_B0012

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663869
display id: annotation1663869
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663869/range1663869/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13541/annotation1663869/range1663869/1
type: Range
persistent identity: range1663869
display id: range1663869
version: 1
direction: direction/forward
top level: BBa_I13541
start: 1
end: 130
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1663876
display id: annotation1663876
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663876/range1663876/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13541/annotation1663876/range1663876/1
type: Range
persistent identity: range1663876
display id: range1663876
version: 1
direction: direction/forward
top level: BBa_I13541
start: 157
end: 876
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663881
display id: annotation1663881
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663881/range1663881/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13541/annotation1663881/range1663881/1
type: Range
persistent identity: range1663881
display id: range1663881
version: 1
direction: direction/forward
top level: BBa_I13541
start: 885
end: 964
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663891
display id: annotation1663891
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663891/range1663891/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13541/annotation1663891/range1663891/1
type: Range
persistent identity: range1663891
display id: range1663891
version: 1
direction: direction/forward
top level: BBa_I13541
start: 973
end: 1013
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663874
display id: annotation1663874
version: 1
top level: BBa_I13541
location: https://synbiohub.org/public/igem/BBa_I13541/annotation1663874/range1663874/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13541/annotation1663874/range1663874/1
type: Range
persistent identity: range1663874
display id: range1663874
version: 1
direction: direction/forward
top level: BBa_I13541
start: 139
end: 150
orientation: inline

BBa_K808014
type: ComponentDefinition
persistent identity: BBa K808014
display id: BBa_K808014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AroY: Catalyzes protocatechuate to catechol
creator: Sascha Hein, Sven Rumpf, Daniel Sachs, Arne Wehling, Philipp Rottmann
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808014_sequence/1

BBa_K895002
type: ComponentDefinition
persistent identity: BBa K895002
display id: BBa_K895002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salty_VgrG
creator: Frank Sargent
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1160_
m user id: 0
owner id: 8083
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a coding part comprising the full open reading frame of the gene encoding the Salmonella enterica LT2 VgrG protein (also known as STM0279 or TssI). VgrG is an important component of bacterial Type VI Secretion Systems and is believed to locate at the very tip of the Type VI secretion device. Normally, therefore, this protein would be considered to be a secreted protein and to be located outside of the bacterial cell.
mutable notes: The Salmonella vgrG gene did not require any modification to meet RFC[10] standard.
mutable provenance: Amplified by PCR from Salmonella LT2 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: vgrG
sequence: https://synbiohub.org/public/igem/BBa_K895002_sequence/1

vgrG
type: SequenceAnnotation
persistent identity: annotation2192729
display id: annotation2192729
version: 1
top level: BBa_K895002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895002/annotation2192729/range2192729/1

https://synbiohub.org/public/igem/BBa_K895002/annotation2192729/range2192729/1
type: Range
persistent identity: range2192729
display id: range2192729
version: 1
direction: direction/forward
top level: BBa_K895002
start: 1
end: 2193
orientation: inline

BBa_K747085
type: ComponentDefinition
persistent identity: BBa K747085
display id: BBa_K747085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 6
sequence: https://synbiohub.org/public/igem/BBa_K747085_sequence/1

CC 6
type: SequenceAnnotation
persistent identity: annotation2197058
display id: annotation2197058
version: 1
top level: BBa_K747085
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747085/annotation2197058/range2197058/1

https://synbiohub.org/public/igem/BBa_K747085/annotation2197058/range2197058/1
type: Range
persistent identity: range2197058
display id: range2197058
version: 1
direction: direction/forward
top level: BBa_K747085
start: 14
end: 211
orientation: inline

BBa_K747020
type: ComponentDefinition
persistent identity: BBa K747020
display id: BBa_K747020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CA2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CA 2
sequence: https://synbiohub.org/public/igem/BBa_K747020_sequence/1

CA 2
type: SequenceAnnotation
persistent identity: annotation2196006
display id: annotation2196006
version: 1
top level: BBa_K747020
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747020/annotation2196006/range2196006/1

https://synbiohub.org/public/igem/BBa_K747020/annotation2196006/range2196006/1
type: Range
persistent identity: range2196006
display id: range2196006
version: 1
direction: direction/forward
top level: BBa_K747020
start: 14
end: 211
orientation: inline

BBa_J202007
type: ComponentDefinition
persistent identity: BBa J202007
display id: BBa_J202007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutnat#8
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -62 relative to the araC transcriptional start site was changed from A to G.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: araI1
sequence annotation: araI2
sequence annotation: Transcription Start
sequence: https://synbiohub.org/public/igem/BBa_J202007_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2178447
display id: annotation2178447
version: 1
top level: BBa_J202007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202007/annotation2178447/range2178447/1

https://synbiohub.org/public/igem/BBa_J202007/annotation2178447/range2178447/1
type: Range
persistent identity: range2178447
display id: range2178447
version: 1
direction: direction/forward
top level: BBa_J202007
start: 50
end: 50
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178445
display id: annotation2178445
version: 1
top level: BBa_J202007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202007/annotation2178445/range2178445/1

https://synbiohub.org/public/igem/BBa_J202007/annotation2178445/range2178445/1
type: Range
persistent identity: range2178445
display id: range2178445
version: 1
direction: direction/forward
top level: BBa_J202007
start: 40
end: 56
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178446
display id: annotation2178446
version: 1
top level: BBa_J202007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202007/annotation2178446/range2178446/1

https://synbiohub.org/public/igem/BBa_J202007/annotation2178446/range2178446/1
type: Range
persistent identity: range2178446
display id: range2178446
version: 1
direction: direction/forward
top level: BBa_J202007
start: 61
end: 77
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178444
display id: annotation2178444
version: 1
top level: BBa_J202007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202007/annotation2178444/range2178444/1

https://synbiohub.org/public/igem/BBa_J202007/annotation2178444/range2178444/1
type: Range
persistent identity: range2178444
display id: range2178444
version: 1
direction: direction/forward
top level: BBa_J202007
start: 112
end: 112
orientation: inline

BBa_S0109
type: ComponentDefinition
persistent identity: BBa S0109
display id: BBa_S0109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0033.C0051
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0033
component: BBa_C0051
sequence annotation: BBa_B0033
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_S0109_sequence/1

BBa_B0033
type: Component
persistent identity: component939856
display id: component939856
version: 1
top level: BBa_S0109
access: public
definition: BBa_B0033

BBa_C0051
type: Component
persistent identity: component939867
display id: component939867
version: 1
top level: BBa_S0109
access: public
definition: cI lam

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939856
display id: annotation939856
version: 1
top level: BBa_S0109
location: https://synbiohub.org/public/igem/BBa_S0109/annotation939856/range939856/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_S0109/annotation939856/range939856/1
type: Range
persistent identity: range939856
display id: range939856
version: 1
direction: direction/forward
top level: BBa_S0109
start: 1
end: 11
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation939867
display id: annotation939867
version: 1
top level: BBa_S0109
location: https://synbiohub.org/public/igem/BBa_S0109/annotation939867/range939867/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_S0109/annotation939867/range939867/1
type: Range
persistent identity: range939867
display id: range939867
version: 1
direction: direction/forward
top level: BBa_S0109
start: 18
end: 767
orientation: inline

BBa_K747087
type: ComponentDefinition
persistent identity: BBa K747087
display id: BBa_K747087
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: Design Notes This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 6
sequence: https://synbiohub.org/public/igem/BBa_K747087_sequence/1

CT 6
type: SequenceAnnotation
persistent identity: annotation2197062
display id: annotation2197062
version: 1
top level: BBa_K747087
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747087/annotation2197062/range2197062/1

https://synbiohub.org/public/igem/BBa_K747087/annotation2197062/range2197062/1
type: Range
persistent identity: range2197062
display id: range2197062
version: 1
direction: direction/forward
top level: BBa_K747087
start: 14
end: 211
orientation: inline

BBa_I6103
type: ComponentDefinition
persistent identity: BBa I6103
display id: BBa_I6103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0074.E0430
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_R0074
component: BBa_E0030
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0074
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6103_sequence/1

BBa_B0034
type: Component
persistent identity: component949788
display id: component949788
version: 1
top level: BBa_I6103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0074
type: Component
persistent identity: component949783
display id: component949783
version: 1
top level: BBa_I6103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0074/1

BBa_E0030
type: Component
persistent identity: component949790
display id: component949790
version: 1
top level: BBa_I6103
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component949795
display id: component949795
version: 1
top level: BBa_I6103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949805
display id: component949805
version: 1
top level: BBa_I6103
access: public
definition: BBa_B0012

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949795
display id: annotation949795
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949795/range949795/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6103/annotation949795/range949795/1
type: Range
persistent identity: range949795
display id: range949795
version: 1
direction: direction/forward
top level: BBa_I6103
start: 835
end: 914
orientation: inline

BBa_R0074
type: SequenceAnnotation
persistent identity: annotation949783
display id: annotation949783
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949783/range949783/1
component: BBa_R0074

https://synbiohub.org/public/igem/BBa_I6103/annotation949783/range949783/1
type: Range
persistent identity: range949783
display id: range949783
version: 1
direction: direction/forward
top level: BBa_I6103
start: 1
end: 77
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949788
display id: annotation949788
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949788/range949788/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6103/annotation949788/range949788/1
type: Range
persistent identity: range949788
display id: range949788
version: 1
direction: direction/forward
top level: BBa_I6103
start: 86
end: 97
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949790
display id: annotation949790
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949790/range949790/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6103/annotation949790/range949790/1
type: Range
persistent identity: range949790
display id: range949790
version: 1
direction: direction/forward
top level: BBa_I6103
start: 104
end: 826
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949805
display id: annotation949805
version: 1
top level: BBa_I6103
location: https://synbiohub.org/public/igem/BBa_I6103/annotation949805/range949805/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6103/annotation949805/range949805/1
type: Range
persistent identity: range949805
display id: range949805
version: 1
direction: direction/forward
top level: BBa_I6103
start: 923
end: 963
orientation: inline

BBa_J202005
type: ComponentDefinition
persistent identity: BBa J202005
display id: BBa_J202005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#6
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -67 relative to the araC transcriptional start site was changed from T to A.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI2
sequence annotation: araI1
sequence annotation: Transcription Start
sequence annotation: Mutation
sequence: https://synbiohub.org/public/igem/BBa_J202005_sequence/1

araI2
type: SequenceAnnotation
persistent identity: annotation2178438
display id: annotation2178438
version: 1
top level: BBa_J202005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202005/annotation2178438/range2178438/1

https://synbiohub.org/public/igem/BBa_J202005/annotation2178438/range2178438/1
type: Range
persistent identity: range2178438
display id: range2178438
version: 1
direction: direction/forward
top level: BBa_J202005
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178437
display id: annotation2178437
version: 1
top level: BBa_J202005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202005/annotation2178437/range2178437/1

https://synbiohub.org/public/igem/BBa_J202005/annotation2178437/range2178437/1
type: Range
persistent identity: range2178437
display id: range2178437
version: 1
direction: direction/forward
top level: BBa_J202005
start: 40
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178436
display id: annotation2178436
version: 1
top level: BBa_J202005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202005/annotation2178436/range2178436/1

https://synbiohub.org/public/igem/BBa_J202005/annotation2178436/range2178436/1
type: Range
persistent identity: range2178436
display id: range2178436
version: 1
direction: direction/forward
top level: BBa_J202005
start: 112
end: 112
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178439
display id: annotation2178439
version: 1
top level: BBa_J202005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202005/annotation2178439/range2178439/1

https://synbiohub.org/public/igem/BBa_J202005/annotation2178439/range2178439/1
type: Range
persistent identity: range2178439
display id: range2178439
version: 1
direction: direction/forward
top level: BBa_J202005
start: 45
end: 45
orientation: inline

BBa_K750000
type: ComponentDefinition
persistent identity: BBa K750000
display id: BBa_K750000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBADGLT:unstable gfp expression device activated by arabinose
creator: Sifan Wang, Yizhen Yan, Jianzhao Chi, Qingshu Wu
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part contains Pbad promoter, RBS of 1.0 strength, GFP(lva) producer and double terminator. GFP(lva) will be produced after arabinose added. In the cell display part of our project, this part is used in part...part...part...
mutable notes: We use the unstable gfp for our project so the degree of fluorescence can be decreased after it get to a top point. In the cell display part, we can achieve the switch of diffrent numbers.
mutable provenance: We built this part by using the biobricks from the dna distribution plates 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K145015
component: BBa_B0015
component: BBa_K206000
component: BBa_B0034
sequence annotation: BBa_K206000
sequence annotation: BBa_K145015
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K750000_sequence/1

BBa_K145015
type: Component
persistent identity: component2184517
display id: component2184517
version: 1
top level: BBa_K750000
access: public
definition: https://synbiohub.org/public/igem/BBa_K145015/1

BBa_B0015
type: Component
persistent identity: component2184524
display id: component2184524
version: 1
top level: BBa_K750000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K206000
type: Component
persistent identity: component2184509
display id: component2184509
version: 1
top level: BBa_K750000
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_B0034
type: Component
persistent identity: component2184511
display id: component2184511
version: 1
top level: BBa_K750000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2184509
display id: annotation2184509
version: 1
top level: BBa_K750000
location: https://synbiohub.org/public/igem/BBa_K750000/annotation2184509/range2184509/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K750000/annotation2184509/range2184509/1
type: Range
persistent identity: range2184509
display id: range2184509
version: 1
direction: direction/reverse
top level: BBa_K750000
start: 1
end: 130
orientation: inline

BBa_K145015
type: SequenceAnnotation
persistent identity: annotation2184517
display id: annotation2184517
version: 1
top level: BBa_K750000
location: https://synbiohub.org/public/igem/BBa_K750000/annotation2184517/range2184517/1
component: BBa_K145015

https://synbiohub.org/public/igem/BBa_K750000/annotation2184517/range2184517/1
type: Range
persistent identity: range2184517
display id: range2184517
version: 1
direction: direction/reverse
top level: BBa_K750000
start: 157
end: 915
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2184524
display id: annotation2184524
version: 1
top level: BBa_K750000
location: https://synbiohub.org/public/igem/BBa_K750000/annotation2184524/range2184524/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K750000/annotation2184524/range2184524/1
type: Range
persistent identity: range2184524
display id: range2184524
version: 1
direction: direction/reverse
top level: BBa_K750000
start: 924
end: 1052
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2184511
display id: annotation2184511
version: 1
top level: BBa_K750000
location: https://synbiohub.org/public/igem/BBa_K750000/annotation2184511/range2184511/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K750000/annotation2184511/range2184511/1
type: Range
persistent identity: range2184511
display id: range2184511
version: 1
direction: direction/reverse
top level: BBa_K750000
start: 139
end: 150
orientation: inline

BBa_K733012
type: ComponentDefinition
persistent identity: BBa K733012
display id: BBa_K733012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylR+PxylA+RBS+ydcE+Ptms+RBS+ydcD: Cell Growth Inhibition Device
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K733010
component: BBa_K733011
sequence annotation: BBa_K733010
sequence annotation: BBa_K733011
sequence: https://synbiohub.org/public/igem/BBa_K733012_sequence/1

BBa_K733010
type: Component
persistent identity: component2183781
display id: component2183781
version: 1
top level: BBa_K733012
access: public
definition: https://synbiohub.org/public/igem/BBa_K733010/1

BBa_K733011
type: Component
persistent identity: component2183778
display id: component2183778
version: 1
top level: BBa_K733012
access: public
definition: https://synbiohub.org/public/igem/BBa_K733011/1

BBa_K733010
type: SequenceAnnotation
persistent identity: annotation2183781
display id: annotation2183781
version: 1
top level: BBa_K733012
location: https://synbiohub.org/public/igem/BBa_K733012/annotation2183781/range2183781/1
component: BBa_K733010

https://synbiohub.org/public/igem/BBa_K733012/annotation2183781/range2183781/1
type: Range
persistent identity: range2183781
display id: range2183781
version: 1
direction: direction/reverse
top level: BBa_K733012
start: 1773
end: 2146
orientation: inline

BBa_K733011
type: SequenceAnnotation
persistent identity: annotation2183778
display id: annotation2183778
version: 1
top level: BBa_K733012
location: https://synbiohub.org/public/igem/BBa_K733012/annotation2183778/range2183778/1
component: BBa_K733011

https://synbiohub.org/public/igem/BBa_K733012/annotation2183778/range2183778/1
type: Range
persistent identity: range2183778
display id: range2183778
version: 1
direction: direction/reverse
top level: BBa_K733012
start: 1
end: 1764
orientation: inline

BBa_J202011
type: ComponentDefinition
persistent identity: BBa J202011
display id: BBa_J202011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#12
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -56 relative to the araC transcriptional start site was changed from A to G.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Start
sequence annotation: Mutation
sequence annotation: araI1
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202011_sequence/1

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178462
display id: annotation2178462
version: 1
top level: BBa_J202011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202011/annotation2178462/range2178462/1

https://synbiohub.org/public/igem/BBa_J202011/annotation2178462/range2178462/1
type: Range
persistent identity: range2178462
display id: range2178462
version: 1
direction: direction/forward
top level: BBa_J202011
start: 112
end: 112
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178461
display id: annotation2178461
version: 1
top level: BBa_J202011
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202011/annotation2178461/range2178461/1

https://synbiohub.org/public/igem/BBa_J202011/annotation2178461/range2178461/1
type: Range
persistent identity: range2178461
display id: range2178461
version: 1
direction: direction/forward
top level: BBa_J202011
start: 56
end: 56
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178463
display id: annotation2178463
version: 1
top level: BBa_J202011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202011/annotation2178463/range2178463/1

https://synbiohub.org/public/igem/BBa_J202011/annotation2178463/range2178463/1
type: Range
persistent identity: range2178463
display id: range2178463
version: 1
direction: direction/forward
top level: BBa_J202011
start: 40
end: 56
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178464
display id: annotation2178464
version: 1
top level: BBa_J202011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202011/annotation2178464/range2178464/1

https://synbiohub.org/public/igem/BBa_J202011/annotation2178464/range2178464/1
type: Range
persistent identity: range2178464
display id: range2178464
version: 1
direction: direction/forward
top level: BBa_J202011
start: 61
end: 77
orientation: inline

BBa_K747059
type: ComponentDefinition
persistent identity: BBa K747059
display id: BBa_K747059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 4
sequence: https://synbiohub.org/public/igem/BBa_K747059_sequence/1

GT 4
type: SequenceAnnotation
persistent identity: annotation2197017
display id: annotation2197017
version: 1
top level: BBa_K747059
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747059/annotation2197017/range2197017/1

https://synbiohub.org/public/igem/BBa_K747059/annotation2197017/range2197017/1
type: Range
persistent identity: range2197017
display id: range2197017
version: 1
direction: direction/forward
top level: BBa_K747059
start: 14
end: 211
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pL)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
mutable provenance: V. fischeri.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: -10
sequence annotation: start
sequence annotation: BBa_R0063
sequence annotation: -35
sequence annotation: Putative LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
direction: direction/forward
top level: lux pL
start: 115
end: 122
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
direction: direction/forward
top level: lux pL
start: 128
end: 128
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
direction: direction/forward
top level: lux pL
start: 89
end: 94
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
direction: direction/forward
top level: lux pL
start: 130
end: 149
orientation: inline

BBa_J04421
type: ComponentDefinition
persistent identity: BBa J04421
display id: BBa_J04421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP Coding Device with Promoter, RBS, Coding Sequence, and Terminator
creator: Kristen DeCelle
created: 2005-06-08T11:00:00Z
modified: 2016-10-14T07:25:32Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 31248
owner id: 326
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: IPTG Inducible Promoter, RBS, ECFP with LVA tag, Terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E0022
component: BBa_B0010
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04421_sequence/1

BBa_B0012
type: Component
persistent identity: component1509538
display id: component1509538
version: 1
top level: BBa_J04421
access: public
definition: BBa_B0012

BBa_E0022
type: Component
persistent identity: component1509520
display id: component1509520
version: 1
top level: BBa_J04421
access: public
definition: ECFP

BBa_B0010
type: Component
persistent identity: component1509528
display id: component1509528
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1509508
display id: component1509508
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1509498
display id: component1509498
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509498
display id: annotation1509498
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509498/range1509498/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04421/annotation1509498/range1509498/1
type: Range
persistent identity: range1509498
display id: range1509498
version: 1
direction: direction/forward
top level: BBa_J04421
start: 1
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509538
display id: annotation1509538
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509538/range1509538/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04421/annotation1509538/range1509538/1
type: Range
persistent identity: range1509538
display id: range1509538
version: 1
direction: direction/forward
top level: BBa_J04421
start: 1085
end: 1125
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1509520
display id: annotation1509520
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509520/range1509520/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J04421/annotation1509520/range1509520/1
type: Range
persistent identity: range1509520
display id: range1509520
version: 1
direction: direction/forward
top level: BBa_J04421
start: 227
end: 988
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509528
display id: annotation1509528
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509528/range1509528/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04421/annotation1509528/range1509528/1
type: Range
persistent identity: range1509528
display id: range1509528
version: 1
direction: direction/forward
top level: BBa_J04421
start: 997
end: 1076
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509508
display id: annotation1509508
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509508/range1509508/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04421/annotation1509508/range1509508/1
type: Range
persistent identity: range1509508
display id: range1509508
version: 1
direction: direction/forward
top level: BBa_J04421
start: 209
end: 220
orientation: inline

BBa_K747079
type: ComponentDefinition
persistent identity: BBa K747079
display id: BBa_K747079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 5
sequence: https://synbiohub.org/public/igem/BBa_K747079_sequence/1

TT 5
type: SequenceAnnotation
persistent identity: annotation2197049
display id: annotation2197049
version: 1
top level: BBa_K747079
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747079/annotation2197049/range2197049/1

https://synbiohub.org/public/igem/BBa_K747079/annotation2197049/range2197049/1
type: Range
persistent identity: range2197049
display id: range2197049
version: 1
direction: direction/forward
top level: BBa_K747079
start: 14
end: 211
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
direction: direction/forward
top level: lux pR
start: 53
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

BBa_J202000
type: ComponentDefinition
persistent identity: BBa J202000
display id: BBa_J202000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD mutant#1
creator: Jiyeon Park
created: 2012-07-18T11:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is mutant of PBAD (BBa_I13453).
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part lacks araO2 site which is essential for araC repressor function, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by random mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: araI1
sequence annotation: araC transcription start
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202000_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2177773
display id: annotation2177773
version: 1
top level: BBa_J202000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202000/annotation2177773/range2177773/1

https://synbiohub.org/public/igem/BBa_J202000/annotation2177773/range2177773/1
type: Range
persistent identity: range2177773
display id: range2177773
version: 1
direction: direction/forward
top level: BBa_J202000
start: 57
end: 57
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2177770
display id: annotation2177770
version: 1
top level: BBa_J202000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202000/annotation2177770/range2177770/1

https://synbiohub.org/public/igem/BBa_J202000/annotation2177770/range2177770/1
type: Range
persistent identity: range2177770
display id: range2177770
version: 1
direction: direction/forward
top level: BBa_J202000
start: 40
end: 56
orientation: inline

araC transcription start
type: SequenceAnnotation
persistent identity: annotation2177772
display id: annotation2177772
version: 1
top level: BBa_J202000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202000/annotation2177772/range2177772/1

https://synbiohub.org/public/igem/BBa_J202000/annotation2177772/range2177772/1
type: Range
persistent identity: range2177772
display id: range2177772
version: 1
direction: direction/forward
top level: BBa_J202000
start: 112
end: 112
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2177771
display id: annotation2177771
version: 1
top level: BBa_J202000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202000/annotation2177771/range2177771/1

https://synbiohub.org/public/igem/BBa_J202000/annotation2177771/range2177771/1
type: Range
persistent identity: range2177771
display id: range2177771
version: 1
direction: direction/forward
top level: BBa_J202000
start: 61
end: 77
orientation: inline

BBa_K876002
type: ComponentDefinition
persistent identity: BBa K876002
display id: BBa_K876002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-LacI
creator: Alec Spinhirne
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter for LacI, repressed by TetR
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K876002_sequence/1

BBa_R0040
type: Component
persistent identity: component2245087
display id: component2245087
version: 1
top level: BBa_K876002
access: public
definition: p(tetR)

BBa_C0012
type: Component
persistent identity: component2245093
display id: component2245093
version: 1
top level: BBa_K876002
access: public
definition: lacI

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2245093
display id: annotation2245093
version: 1
top level: BBa_K876002
location: https://synbiohub.org/public/igem/BBa_K876002/annotation2245093/range2245093/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K876002/annotation2245093/range2245093/1
type: Range
persistent identity: range2245093
display id: range2245093
version: 1
direction: direction/forward
top level: BBa_K876002
start: 61
end: 1188
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2245087
display id: annotation2245087
version: 1
top level: BBa_K876002
location: https://synbiohub.org/public/igem/BBa_K876002/annotation2245087/range2245087/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876002/annotation2245087/range2245087/1
type: Range
persistent identity: range2245087
display id: range2245087
version: 1
direction: direction/forward
top level: BBa_K876002
start: 1
end: 54
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: -10
sequence annotation: TetR 2
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

araC-CysE
type: ComponentDefinition
persistent identity: BBa K731020
display id: BBa_K731020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible araC-pBAD promoter regulating WT CysE
creator: Jason Fontana
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The CysE gene (K731000) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose.
mutable notes: CysE was inserted after araC-pBAD in his vector, cutting the former with X&P and the latter with S&P.
mutable provenance: K731200, K731000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K731201
component: BBa_K731000
sequence annotation: BBa_K731201
sequence annotation: BBa_K731000
sequence: https://synbiohub.org/public/igem/BBa_K731020_sequence/1

BBa_K731201
type: Component
persistent identity: component2331169
display id: component2331169
version: 1
top level: araC-CysE
access: public
definition: https://synbiohub.org/public/igem/BBa_K731201/1

BBa_K731000
type: Component
persistent identity: component2331174
display id: component2331174
version: 1
top level: araC-CysE
access: public
definition: https://synbiohub.org/public/igem/BBa_K731000/1

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2331169
display id: annotation2331169
version: 1
top level: araC-CysE
location: https://synbiohub.org/public/igem/BBa_K731020/annotation2331169/range2331169/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K731020/annotation2331169/range2331169/1
type: Range
persistent identity: range2331169
display id: range2331169
version: 1
direction: direction/reverse
top level: araC-CysE
start: 1
end: 1203
orientation: inline

BBa_K731000
type: SequenceAnnotation
persistent identity: annotation2331174
display id: annotation2331174
version: 1
top level: araC-CysE
location: https://synbiohub.org/public/igem/BBa_K731020/annotation2331174/range2331174/1
component: BBa_K731000

https://synbiohub.org/public/igem/BBa_K731020/annotation2331174/range2331174/1
type: Range
persistent identity: range2331174
display id: range2331174
version: 1
direction: direction/reverse
top level: araC-CysE
start: 1212
end: 2050
orientation: inline

BBa_K759012
type: ComponentDefinition
persistent identity: BBa K759012
display id: BBa_K759012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.
creator: Kenta Mine
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
group u list: _1010_
m user id: 0
owner id: 10980
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In the presence of L-arabinose, pBAD promoter is induced and then wildtype Antigen 43 (Ag43) protein and eCFP (engineered cyan fluorescent protein)is produced. The wildtype Ag43 have 6 PstI sites. eCFP locates in the upstream of Ag43 for estimating the relationship between expressed protein amount and aggregation efficiency.
mutable notes: 6 PstI sites exists in Ag43 coding site so we assembled this composite part without using PstI.
mutable provenance: Ag43(BBA_K346007) was derived from E. coli K-12 strain by Peking 2010 iGEM.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0020
component: BBa_I0500
component: BBa_B0034
component: BBa_B0015
component: BBa_K346007
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_I0500
sequence annotation: BBa_K346007
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K759012_sequence/1

BBa_E0020
type: Component
persistent identity: component2188721
display id: component2188721
version: 1
top level: BBa_K759012
access: public
definition: ecfp

BBa_I0500
type: Component
persistent identity: component2188718
display id: component2188718
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2188723
display id: component2188723
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2188732
display id: component2188732
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K346007
type: Component
persistent identity: component2188725
display id: component2188725
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_K346007/1

BBa_B0034
type: Component
persistent identity: component2188720
display id: component2188720
version: 1
top level: BBa_K759012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188723
display id: annotation2188723
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188723/range2188723/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759012/annotation2188723/range2188723/1
type: Range
persistent identity: range2188723
display id: range2188723
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1968
end: 1979
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2188721
display id: annotation2188721
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188721/range2188721/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_K759012/annotation2188721/range2188721/1
type: Range
persistent identity: range2188721
display id: range2188721
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1237
end: 1959
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2188718
display id: annotation2188718
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188718/range2188718/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K759012/annotation2188718/range2188718/1
type: Range
persistent identity: range2188718
display id: range2188718
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1
end: 1210
orientation: inline

BBa_K346007
type: SequenceAnnotation
persistent identity: annotation2188725
display id: annotation2188725
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188725/range2188725/1
component: BBa_K346007

https://synbiohub.org/public/igem/BBa_K759012/annotation2188725/range2188725/1
type: Range
persistent identity: range2188725
display id: range2188725
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1986
end: 5105
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188720
display id: annotation2188720
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188720/range2188720/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759012/annotation2188720/range2188720/1
type: Range
persistent identity: range2188720
display id: range2188720
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 1219
end: 1230
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2188732
display id: annotation2188732
version: 1
top level: BBa_K759012
location: https://synbiohub.org/public/igem/BBa_K759012/annotation2188732/range2188732/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K759012/annotation2188732/range2188732/1
type: Range
persistent identity: range2188732
display id: range2188732
version: 1
direction: direction/reverse
top level: BBa_K759012
start: 5114
end: 5242
orientation: inline

BBa_K747027
type: ComponentDefinition
persistent identity: BBa K747027
display id: BBa_K747027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 2
sequence: https://synbiohub.org/public/igem/BBa_K747027_sequence/1

GT 2
type: SequenceAnnotation
persistent identity: annotation2196136
display id: annotation2196136
version: 1
top level: BBa_K747027
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747027/annotation2196136/range2196136/1

https://synbiohub.org/public/igem/BBa_K747027/annotation2196136/range2196136/1
type: Range
persistent identity: range2196136
display id: range2196136
version: 1
direction: direction/forward
top level: BBa_K747027
start: 14
end: 211
orientation: inline

BBa_K784006
type: ComponentDefinition
persistent identity: BBa K784006
display id: BBa_K784006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline riboswitch 12.1 + mCherry
creator: Ilya Vainberg Slutskin
created: 2012-09-12T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a direct fusion of the theophylline riboswitch (BBa_K784005) and the mCherry protein (BBa_J06504) which was amplified from BBa_J06702. The part was generated by [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr]. This part was to be used in order to characterize the effect of theophylline concentration on the level of translation of the mCherry protein.
mutable notes: The theophylline riboswitch had to be directly fused to the start codon of the mCherry protein. To achieve this, [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr] was used.
mutable provenance: This part is a composite part of BBa_K784005 and BBa_J06504
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J06504
component: BBa_K784005
sequence annotation: BBa_K784005
sequence annotation: BBa_J06504
sequence: https://synbiohub.org/public/igem/BBa_K784006_sequence/1

BBa_J06504
type: Component
persistent identity: component2182910
display id: component2182910
version: 1
top level: BBa_K784006
access: public
definition: mCherry

BBa_K784005
type: Component
persistent identity: component2182907
display id: component2182907
version: 1
top level: BBa_K784006
access: public
definition: https://synbiohub.org/public/igem/BBa_K784005/1

BBa_K784005
type: SequenceAnnotation
persistent identity: annotation2182907
display id: annotation2182907
version: 1
top level: BBa_K784006
location: https://synbiohub.org/public/igem/BBa_K784006/annotation2182907/range2182907/1
component: BBa_K784005

https://synbiohub.org/public/igem/BBa_K784006/annotation2182907/range2182907/1
type: Range
persistent identity: range2182907
display id: range2182907
version: 1
direction: direction/reverse
top level: BBa_K784006
start: 1
end: 74
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation2182910
display id: annotation2182910
version: 1
top level: BBa_K784006
location: https://synbiohub.org/public/igem/BBa_K784006/annotation2182910/range2182910/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_K784006/annotation2182910/range2182910/1
type: Range
persistent identity: range2182910
display id: range2182910
version: 1
direction: direction/reverse
top level: BBa_K784006
start: 75
end: 788
orientation: inline

BBa_K934023
type: ComponentDefinition
persistent identity: BBa K934023
display id: BBa_K934023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL->3OC12HSL
creator: Naoki Tsukuda
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1199_
m user id: 0
owner id: 14032
owning group id: 9
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This part generates LasI(which generate 3OC12HSL) in the presence of 3OC6HSL. We constructed it with (???) and (Plux,LasI part name).
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K934022
component: BBa_S03119
component: BBa_C0078
sequence annotation: BBa_C0078
sequence annotation: BBa_K934022
sequence annotation: BBa_S03119
sequence: https://synbiohub.org/public/igem/BBa_K934023_sequence/1

BBa_K934022
type: Component
persistent identity: component2184094
display id: component2184094
version: 1
top level: BBa_K934023
access: public
definition: https://synbiohub.org/public/igem/BBa_K934022/1

BBa_S03119
type: Component
persistent identity: component2184114
display id: component2184114
version: 1
top level: BBa_K934023
access: public
definition: https://synbiohub.org/public/igem/BBa_S03119/1

BBa_C0078
type: Component
persistent identity: component2184086
display id: component2184086
version: 1
top level: BBa_K934023
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2184086
display id: annotation2184086
version: 1
top level: BBa_K934023
location: https://synbiohub.org/public/igem/BBa_K934023/annotation2184086/range2184086/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K934023/annotation2184086/range2184086/1
type: Range
persistent identity: range2184086
display id: range2184086
version: 1
direction: direction/reverse
top level: BBa_K934023
start: 85
end: 726
orientation: inline

BBa_K934022
type: SequenceAnnotation
persistent identity: annotation2184094
display id: annotation2184094
version: 1
top level: BBa_K934023
location: https://synbiohub.org/public/igem/BBa_K934023/annotation2184094/range2184094/1
component: BBa_K934022

https://synbiohub.org/public/igem/BBa_K934023/annotation2184094/range2184094/1
type: Range
persistent identity: range2184094
display id: range2184094
version: 1
direction: direction/reverse
top level: BBa_K934023
start: 1
end: 798
orientation: inline

BBa_S03119
type: SequenceAnnotation
persistent identity: annotation2184114
display id: annotation2184114
version: 1
top level: BBa_K934023
location: https://synbiohub.org/public/igem/BBa_K934023/annotation2184114/range2184114/1
component: BBa_S03119

https://synbiohub.org/public/igem/BBa_K934023/annotation2184114/range2184114/1
type: Range
persistent identity: range2184114
display id: range2184114
version: 1
direction: direction/reverse
top level: BBa_K934023
start: 807
end: 1804
orientation: inline

BBa_K737033
type: ComponentDefinition
persistent identity: BBa K737033
display id: BBa_K737033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: galK is the leader sequence of galactokinase (GalK)
creator: Peiran Zhang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: galK is the leader sequence of galactokinase (GalK),and Spot42 sRNA controls the synthesis of the galactokinase (GalK) in response to the availability of glucose in the environment,by blocking the recognition of antiSD motif to RBS.The repression is modest(about 2.6 fold),maybe there is no subsequent degradation caused by the major degradosome,RNaesE and RNaseIII. Note:The ClaI site is blocked by GATC methylation due to a mistake.It should be amplified by PCR to get rid of dam methylation before ClaI degestion
mutable notes: No
mutable provenance: Reference: [1] Johannes H. Urban and Jo?? rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3 [2] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K737033_sequence/1

BBa_K747017
type: ComponentDefinition
persistent identity: BBa K747017
display id: BBa_K747017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 2
sequence: https://synbiohub.org/public/igem/BBa_K747017_sequence/1

AC 2
type: SequenceAnnotation
persistent identity: annotation2195966
display id: annotation2195966
version: 1
top level: BBa_K747017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747017/annotation2195966/range2195966/1

https://synbiohub.org/public/igem/BBa_K747017/annotation2195966/range2195966/1
type: Range
persistent identity: range2195966
display id: range2195966
version: 1
direction: direction/forward
top level: BBa_K747017
start: 14
end: 211
orientation: inline

BBa_K876067
type: ComponentDefinition
persistent identity: BBa K876067
display id: BBa_K876067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Term-pLac-LuxR
creator: Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: true
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TT-pLac-LuxR
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0062
component: BBa_B0015
component: BBa_R0010
sequence annotation: BBa_C0062
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K876067_sequence/1

BBa_C0062
type: Component
persistent identity: component2251310
display id: component2251310
version: 1
top level: BBa_K876067
access: public
definition: luxr

BBa_B0015
type: Component
persistent identity: component2251300
display id: component2251300
version: 1
top level: BBa_K876067
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: Component
persistent identity: component2251301
display id: component2251301
version: 1
top level: BBa_K876067
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2251310
display id: annotation2251310
version: 1
top level: BBa_K876067
location: https://synbiohub.org/public/igem/BBa_K876067/annotation2251310/range2251310/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K876067/annotation2251310/range2251310/1
type: Range
persistent identity: range2251310
display id: range2251310
version: 1
direction: direction/forward
top level: BBa_K876067
start: 344
end: 1099
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2251301
display id: annotation2251301
version: 1
top level: BBa_K876067
location: https://synbiohub.org/public/igem/BBa_K876067/annotation2251301/range2251301/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876067/annotation2251301/range2251301/1
type: Range
persistent identity: range2251301
display id: range2251301
version: 1
direction: direction/forward
top level: BBa_K876067
start: 138
end: 337
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251300
display id: annotation2251300
version: 1
top level: BBa_K876067
location: https://synbiohub.org/public/igem/BBa_K876067/annotation2251300/range2251300/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876067/annotation2251300/range2251300/1
type: Range
persistent identity: range2251300
display id: range2251300
version: 1
direction: direction/reverse
top level: BBa_K876067
start: 1
end: 129
orientation: inline

BBa_K747068
type: ComponentDefinition
persistent identity: BBa K747068
display id: BBa_K747068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CA5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CA 5
sequence: https://synbiohub.org/public/igem/BBa_K747068_sequence/1

CA 5
type: SequenceAnnotation
persistent identity: annotation2197031
display id: annotation2197031
version: 1
top level: BBa_K747068
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747068/annotation2197031/range2197031/1

https://synbiohub.org/public/igem/BBa_K747068/annotation2197031/range2197031/1
type: Range
persistent identity: range2197031
display id: range2197031
version: 1
direction: direction/forward
top level: BBa_K747068
start: 14
end: 211
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R1062
sequence annotation: start
sequence annotation: LuxR/HSL
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 56
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
direction: direction/forward
top level: lux pR
start: 54
end: 54
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

BBa_K850002
type: ComponentDefinition
persistent identity: BBa K850002
display id: BBa_K850002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UAP1 gene from candida albicans
creator: Min-Gyu Kim
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1110_
m user id: 0
owner id: 10645
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: UDP-N-acetylglucosamine pyrophosphorylase, catalyzes biosynthesis of UDP-N-acetylglucosamine from UTP and N-acetylglucosamine 1-phosphate; functional homolog of S. cerevisiae Qri1p; alkaline upregulated. This gene is part of the pathway that synthesizes the sugar subunit of the polymer chitin.
mutable notes: Part was synthesized from <500bp overlapping Gibson Assembly fragments
mutable provenance: candida genome database orf19.4265 (C. albicans SC5314)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K850002_sequence/1

BBa_K779501
type: ComponentDefinition
persistent identity: BBa K779501
display id: BBa_K779501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long reporter top strand MammoBlock
creator: Kristjan Eerik Kaseniit
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1033_
m user id: 0
owner id: 12684
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Reporter top strand. Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts). IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmU mCmUmC mCmCmA mCmCmA /3AlexF488N/
mutable notes: Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
mutable provenance: De novo design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Domain Sa
sequence: https://synbiohub.org/public/igem/BBa_K779501_sequence/1

Domain Sa
type: SequenceAnnotation
persistent identity: annotation2204235
display id: annotation2204235
version: 1
top level: BBa_K779501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779501/annotation2204235/range2204235/1

https://synbiohub.org/public/igem/BBa_K779501/annotation2204235/range2204235/1
type: Range
persistent identity: range2204235
display id: range2204235
version: 1
direction: direction/forward
top level: BBa_K779501
start: 1
end: 20
orientation: inline

TD0.01
type: ComponentDefinition
persistent identity: BBa K750012
display id: BBa_K750012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIME DELAY0.01:LuxI(RBS0.01)->LuxR->LuxPR->GFP
creator: Sifan Wang,Ruosang Qiu,Jianzhao Chi,Muxin Yu
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains part8 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
mutable notes: editing...
mutable provenance: We built this part by biobricks from the DNA distribution kit plates 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K750007
component: BBa_K750005
sequence annotation: BBa_K750007
sequence annotation: BBa_K750005
sequence: https://synbiohub.org/public/igem/BBa_K750012_sequence/1

BBa_K750007
type: Component
persistent identity: component2312814
display id: component2312814
version: 1
top level: TD0.01
access: public
definition: https://synbiohub.org/public/igem/BBa_K750007/1

BBa_K750005
type: Component
persistent identity: component2312777
display id: component2312777
version: 1
top level: TD0.01
access: public
definition: https://synbiohub.org/public/igem/BBa_K750005/1

BBa_K750007
type: SequenceAnnotation
persistent identity: annotation2312814
display id: annotation2312814
version: 1
top level: TD0.01
location: https://synbiohub.org/public/igem/BBa_K750012/annotation2312814/range2312814/1
component: BBa_K750007

https://synbiohub.org/public/igem/BBa_K750012/annotation2312814/range2312814/1
type: Range
persistent identity: range2312814
display id: range2312814
version: 1
direction: direction/reverse
top level: TD0.01
start: 944
end: 2963
orientation: inline

BBa_K750005
type: SequenceAnnotation
persistent identity: annotation2312777
display id: annotation2312777
version: 1
top level: TD0.01
location: https://synbiohub.org/public/igem/BBa_K750012/annotation2312777/range2312777/1
component: BBa_K750005

https://synbiohub.org/public/igem/BBa_K750012/annotation2312777/range2312777/1
type: Range
persistent identity: range2312777
display id: range2312777
version: 1
direction: direction/reverse
top level: TD0.01
start: 1
end: 935
orientation: inline

BBa_K847101
type: ComponentDefinition
persistent identity: BBa K847101
display id: BBa_K847101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Engineered FliC with insertable expression region
creator: Julia Borden, Michelle Yu, Bella Okiddy
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1107_
m user id: 0
owner id: 11092
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_B0015
component: BBa_B0030
component: BBa_K847102
sequence annotation: BBa_B0015
sequence annotation: BBa_J23100
sequence annotation: BBa_K847102
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K847101_sequence/1

BBa_J23100
type: Component
persistent identity: component2209424
display id: component2209424
version: 1
top level: BBa_K847101
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0015
type: Component
persistent identity: component2209438
display id: component2209438
version: 1
top level: BBa_K847101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0030
type: Component
persistent identity: component2209426
display id: component2209426
version: 1
top level: BBa_K847101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K847102
type: Component
persistent identity: component2209431
display id: component2209431
version: 1
top level: BBa_K847101
access: public
definition: https://synbiohub.org/public/igem/BBa_K847102/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2209438
display id: annotation2209438
version: 1
top level: BBa_K847101
location: https://synbiohub.org/public/igem/BBa_K847101/annotation2209438/range2209438/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K847101/annotation2209438/range2209438/1
type: Range
persistent identity: range2209438
display id: range2209438
version: 1
direction: direction/reverse
top level: BBa_K847101
start: 976
end: 1104
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2209424
display id: annotation2209424
version: 1
top level: BBa_K847101
location: https://synbiohub.org/public/igem/BBa_K847101/annotation2209424/range2209424/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K847101/annotation2209424/range2209424/1
type: Range
persistent identity: range2209424
display id: range2209424
version: 1
direction: direction/reverse
top level: BBa_K847101
start: 1
end: 35
orientation: inline

BBa_K847102
type: SequenceAnnotation
persistent identity: annotation2209431
display id: annotation2209431
version: 1
top level: BBa_K847101
location: https://synbiohub.org/public/igem/BBa_K847101/annotation2209431/range2209431/1
component: BBa_K847102

https://synbiohub.org/public/igem/BBa_K847101/annotation2209431/range2209431/1
type: Range
persistent identity: range2209431
display id: range2209431
version: 1
direction: direction/reverse
top level: BBa_K847101
start: 65
end: 967
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2209426
display id: annotation2209426
version: 1
top level: BBa_K847101
location: https://synbiohub.org/public/igem/BBa_K847101/annotation2209426/range2209426/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K847101/annotation2209426/range2209426/1
type: Range
persistent identity: range2209426
display id: range2209426
version: 1
direction: direction/forward
top level: BBa_K847101
start: 44
end: 58
orientation: inline

BBa_K747003
type: ComponentDefinition
persistent identity: BBa K747003
display id: BBa_K747003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT4
sequence: https://synbiohub.org/public/igem/BBa_K747003_sequence/1

AT4
type: SequenceAnnotation
persistent identity: annotation2195782
display id: annotation2195782
version: 1
top level: BBa_K747003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747003/annotation2195782/range2195782/1

https://synbiohub.org/public/igem/BBa_K747003/annotation2195782/range2195782/1
type: Range
persistent identity: range2195782
display id: range2195782
version: 1
direction: direction/forward
top level: BBa_K747003
start: 12
end: 209
orientation: inline

BBa_K733002
type: ComponentDefinition
persistent identity: BBa K733002
display id: BBa_K733002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylR+PxylA: A xylose inducible promoter with its transcriptional regulator.
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A, -35
sequence annotation: Sigma A, -10
sequence annotation: xylR
sequence: https://synbiohub.org/public/igem/BBa_K733002_sequence/1

Sigma A, -35
type: SequenceAnnotation
persistent identity: annotation2193604
display id: annotation2193604
version: 1
top level: BBa_K733002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K733002/annotation2193604/range2193604/1

https://synbiohub.org/public/igem/BBa_K733002/annotation2193604/range2193604/1
type: Range
persistent identity: range2193604
display id: range2193604
version: 1
direction: direction/forward
top level: BBa_K733002
start: 1277
end: 1282
orientation: inline

Sigma A, -10
type: SequenceAnnotation
persistent identity: annotation2193603
display id: annotation2193603
version: 1
top level: BBa_K733002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K733002/annotation2193603/range2193603/1

https://synbiohub.org/public/igem/BBa_K733002/annotation2193603/range2193603/1
type: Range
persistent identity: range2193603
display id: range2193603
version: 1
direction: direction/forward
top level: BBa_K733002
start: 1300
end: 1305
orientation: inline

xylR
type: SequenceAnnotation
persistent identity: annotation2193589
display id: annotation2193589
version: 1
top level: BBa_K733002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K733002/annotation2193589/range2193589/1

https://synbiohub.org/public/igem/BBa_K733002/annotation2193589/range2193589/1
type: Range
persistent identity: range2193589
display id: range2193589
version: 1
direction: direction/reverse
top level: BBa_K733002
start: 7
end: 1173
orientation: inline

BBa_K747021
type: ComponentDefinition
persistent identity: BBa K747021
display id: BBa_K747021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 2
sequence: https://synbiohub.org/public/igem/BBa_K747021_sequence/1

CC 2
type: SequenceAnnotation
persistent identity: annotation2196025
display id: annotation2196025
version: 1
top level: BBa_K747021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747021/annotation2196025/range2196025/1

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type: Range
persistent identity: range2196025
display id: range2196025
version: 1
direction: direction/forward
top level: BBa_K747021
start: 14
end: 211
orientation: inline

BBa_I6070
type: ComponentDefinition
persistent identity: BBa I6070
display id: BBa_I6070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0053.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0012
component: BBa_R0053
component: BBa_E0032
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0053
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I6070_sequence/1

BBa_B0010
type: Component
persistent identity: component949216
display id: component949216
version: 1
top level: BBa_I6070
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component949226
display id: component949226
version: 1
top level: BBa_I6070
access: public
definition: BBa_B0012

BBa_R0053
type: Component
persistent identity: component949186
display id: component949186
version: 1
top level: BBa_I6070
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_E0032
type: Component
persistent identity: component949208
display id: component949208
version: 1
top level: BBa_I6070
access: public
definition: YFP

BBa_B0034
type: Component
persistent identity: component949196
display id: component949196
version: 1
top level: BBa_I6070
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949226
display id: annotation949226
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949226/range949226/1
component: BBa_B0012

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type: Range
persistent identity: range949226
display id: range949226
version: 1
direction: direction/forward
top level: BBa_I6070
start: 939
end: 979
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation949186
display id: annotation949186
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949186/range949186/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I6070/annotation949186/range949186/1
type: Range
persistent identity: range949186
display id: range949186
version: 1
direction: direction/forward
top level: BBa_I6070
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949196
display id: annotation949196
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949196/range949196/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6070/annotation949196/range949196/1
type: Range
persistent identity: range949196
display id: range949196
version: 1
direction: direction/forward
top level: BBa_I6070
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949216
display id: annotation949216
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949216/range949216/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6070/annotation949216/range949216/1
type: Range
persistent identity: range949216
display id: range949216
version: 1
direction: direction/forward
top level: BBa_I6070
start: 851
end: 930
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949208
display id: annotation949208
version: 1
top level: BBa_I6070
location: https://synbiohub.org/public/igem/BBa_I6070/annotation949208/range949208/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6070/annotation949208/range949208/1
type: Range
persistent identity: range949208
display id: range949208
version: 1
direction: direction/forward
top level: BBa_I6070
start: 81
end: 842
orientation: inline

BBa_K876060
type: ComponentDefinition
persistent identity: BBa K876060
display id: BBa_K876060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Term-plasR-RBS-CFP-Term-Term
creator: Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pLasR is off by default and can be activated by LasR.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0422
component: BBa_R0079
component: BBa_B0015
sequence annotation: BBa_E0422
sequence annotation: BBa_B0015
sequence annotation: BBa_R0079
sequence: https://synbiohub.org/public/igem/BBa_K876060_sequence/1

BBa_E0422
type: Component
persistent identity: component2206382
display id: component2206382
version: 1
top level: BBa_K876060
access: public
definition: BBa_E0422

BBa_R0079
type: Component
persistent identity: component2206368
display id: component2206368
version: 1
top level: BBa_K876060
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0015
type: Component
persistent identity: component2206363
display id: component2206363
version: 1
top level: BBa_K876060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2206382
display id: annotation2206382
version: 1
top level: BBa_K876060
location: https://synbiohub.org/public/igem/BBa_K876060/annotation2206382/range2206382/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_K876060/annotation2206382/range2206382/1
type: Range
persistent identity: range2206382
display id: range2206382
version: 1
direction: direction/reverse
top level: BBa_K876060
start: 303
end: 1219
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2206363
display id: annotation2206363
version: 1
top level: BBa_K876060
location: https://synbiohub.org/public/igem/BBa_K876060/annotation2206363/range2206363/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876060/annotation2206363/range2206363/1
type: Range
persistent identity: range2206363
display id: range2206363
version: 1
direction: direction/reverse
top level: BBa_K876060
start: 1
end: 129
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2206368
display id: annotation2206368
version: 1
top level: BBa_K876060
location: https://synbiohub.org/public/igem/BBa_K876060/annotation2206368/range2206368/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K876060/annotation2206368/range2206368/1
type: Range
persistent identity: range2206368
display id: range2206368
version: 1
direction: direction/reverse
top level: BBa_K876060
start: 138
end: 294
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
mutable provenance: AF196486 from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aiiA
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0060
sequence annotation: 2
sequence annotation: LVA
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
direction: direction/forward
top level: aiiA
start: 790
end: 814
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 789
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
direction: direction/forward
top level: aiiA
start: 784
end: 789
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
direction: direction/forward
top level: aiiA
start: 751
end: 783
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 3
orientation: inline

BBa_K801093
type: ComponentDefinition
persistent identity: BBa K801093
display id: BBa_K801093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4-coumarate--coenzyme A ligase (4CL) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4-coumarate--coenzyme A ligase (4CL) coding region from ''Arabidopsis thaliana''.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: Start codon
sequence annotation: 4CL
sequence: https://synbiohub.org/public/igem/BBa_K801093_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202141
display id: annotation2202141
version: 1
top level: BBa_K801093
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1
type: Range
persistent identity: range2202141
display id: range2202141
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1684
end: 1686
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202140
display id: annotation2202140
version: 1
top level: BBa_K801093
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1
type: Range
persistent identity: range2202140
display id: range2202140
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1
end: 3
orientation: inline

4CL
type: SequenceAnnotation
persistent identity: annotation2202143
display id: annotation2202143
version: 1
top level: BBa_K801093
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1
type: Range
persistent identity: range2202143
display id: range2202143
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1
end: 1683
orientation: inline

BBa_K777117
type: ComponentDefinition
persistent identity: BBa K777117
display id: BBa_K777117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB
creator: Team Goettingen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
mutable notes: * motA was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAGAATCAAGCGCATCC ** Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACCTCGGTTCGGCTGATGG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MotB coding region
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K777117_sequence/1

MotB coding region
type: SequenceAnnotation
persistent identity: annotation2184525
display id: annotation2184525
version: 1
top level: BBa_K777117
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184525/range2184525/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184525/range2184525/1
type: Range
persistent identity: range2184525
display id: range2184525
version: 1
direction: direction/forward
top level: BBa_K777117
start: 1
end: 927
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2184527
display id: annotation2184527
version: 1
top level: BBa_K777117
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184527/range2184527/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184527/range2184527/1
type: Range
persistent identity: range2184527
display id: range2184527
version: 1
direction: direction/forward
top level: BBa_K777117
start: 927
end: 927
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2184526
display id: annotation2184526
version: 1
top level: BBa_K777117
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184526/range2184526/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184526/range2184526/1
type: Range
persistent identity: range2184526
display id: range2184526
version: 1
direction: direction/forward
top level: BBa_K777117
start: 1
end: 1
orientation: inline

BBa_K801092
type: ComponentDefinition
persistent identity: BBa K801092
display id: BBa_K801092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4-coumarate - coenzym A ligase (4CL) coding region from ''Arabidopsis thaliana'' preceeded by the yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 4CL
sequence annotation: Stop codon
sequence annotation: Consensus sequence
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801092_sequence/1

4CL
type: SequenceAnnotation
persistent identity: annotation2201482
display id: annotation2201482
version: 1
top level: BBa_K801092
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2201482/range2201482/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2201482/range2201482/1
type: Range
persistent identity: range2201482
display id: range2201482
version: 1
direction: direction/forward
top level: BBa_K801092
start: 7
end: 1689
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202114
display id: annotation2202114
version: 1
top level: BBa_K801092
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2202114/range2202114/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2202114/range2202114/1
type: Range
persistent identity: range2202114
display id: range2202114
version: 1
direction: direction/forward
top level: BBa_K801092
start: 1690
end: 1692
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2196267
display id: annotation2196267
version: 1
top level: BBa_K801092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2196267/range2196267/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2196267/range2196267/1
type: Range
persistent identity: range2196267
display id: range2196267
version: 1
direction: direction/forward
top level: BBa_K801092
start: 1
end: 6
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201481
display id: annotation2201481
version: 1
top level: BBa_K801092
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2201481/range2201481/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2201481/range2201481/1
type: Range
persistent identity: range2201481
display id: range2201481
version: 1
direction: direction/forward
top level: BBa_K801092
start: 7
end: 9
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0010
component: BBa_R0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_K876009
type: ComponentDefinition
persistent identity: BBa K876009
display id: BBa_K876009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR-DT-pLux-TetR
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inducible promoter for TetR
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0040
component: BBa_C0062
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_B0015
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K876009_sequence/1

BBa_B0015
type: Component
persistent identity: component2259759
display id: component2259759
version: 1
top level: BBa_K876009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0040
type: Component
persistent identity: component2259768
display id: component2259768
version: 1
top level: BBa_K876009
access: public
definition: tetR

BBa_C0062
type: Component
persistent identity: component2259749
display id: component2259749
version: 1
top level: BBa_K876009
access: public
definition: luxr

BBa_R0062
type: Component
persistent identity: component2259761
display id: component2259761
version: 1
top level: BBa_K876009
access: public
definition: lux pR

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2259761
display id: annotation2259761
version: 1
top level: BBa_K876009
location: https://synbiohub.org/public/igem/BBa_K876009/annotation2259761/range2259761/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K876009/annotation2259761/range2259761/1
type: Range
persistent identity: range2259761
display id: range2259761
version: 1
direction: direction/forward
top level: BBa_K876009
start: 927
end: 981
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2259749
display id: annotation2259749
version: 1
top level: BBa_K876009
location: https://synbiohub.org/public/igem/BBa_K876009/annotation2259749/range2259749/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K876009/annotation2259749/range2259749/1
type: Range
persistent identity: range2259749
display id: range2259749
version: 1
direction: direction/forward
top level: BBa_K876009
start: 1
end: 756
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259759
display id: annotation2259759
version: 1
top level: BBa_K876009
location: https://synbiohub.org/public/igem/BBa_K876009/annotation2259759/range2259759/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876009/annotation2259759/range2259759/1
type: Range
persistent identity: range2259759
display id: range2259759
version: 1
direction: direction/reverse
top level: BBa_K876009
start: 790
end: 918
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2259768
display id: annotation2259768
version: 1
top level: BBa_K876009
location: https://synbiohub.org/public/igem/BBa_K876009/annotation2259768/range2259768/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876009/annotation2259768/range2259768/1
type: Range
persistent identity: range2259768
display id: range2259768
version: 1
direction: direction/reverse
top level: BBa_K876009
start: 988
end: 1672
orientation: inline

TD1.0
type: ComponentDefinition
persistent identity: BBa K750009
display id: BBa_K750009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP
creator: Sifan Wang,Ruosang Qiu,Xinyi Yao,Qingshu Wu
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part contains BBa-K750001 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
mutable notes: editing
mutable provenance: We built this part by biobricks from the DNA distribution kit plates 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K750007
component: BBa_K750001
sequence annotation: BBa_K750001
sequence annotation: BBa_K750007
sequence: https://synbiohub.org/public/igem/BBa_K750009_sequence/1

BBa_K750007
type: Component
persistent identity: component2312646
display id: component2312646
version: 1
top level: TD1.0
access: public
definition: https://synbiohub.org/public/igem/BBa_K750007/1

BBa_K750001
type: Component
persistent identity: component2312609
display id: component2312609
version: 1
top level: TD1.0
access: public
definition: https://synbiohub.org/public/igem/BBa_K750001/1

BBa_K750001
type: SequenceAnnotation
persistent identity: annotation2312609
display id: annotation2312609
version: 1
top level: TD1.0
location: https://synbiohub.org/public/igem/BBa_K750009/annotation2312609/range2312609/1
component: BBa_K750001

https://synbiohub.org/public/igem/BBa_K750009/annotation2312609/range2312609/1
type: Range
persistent identity: range2312609
display id: range2312609
version: 1
direction: direction/reverse
top level: TD1.0
start: 1
end: 936
orientation: inline

BBa_K750007
type: SequenceAnnotation
persistent identity: annotation2312646
display id: annotation2312646
version: 1
top level: TD1.0
location: https://synbiohub.org/public/igem/BBa_K750009/annotation2312646/range2312646/1
component: BBa_K750007

https://synbiohub.org/public/igem/BBa_K750009/annotation2312646/range2312646/1
type: Range
persistent identity: range2312646
display id: range2312646
version: 1
direction: direction/reverse
top level: TD1.0
start: 945
end: 2964
orientation: inline

BBa_K936013
type: ComponentDefinition
persistent identity: BBa K936013
display id: BBa_K936013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pelB-LC-Cutinase fusion protein
creator: Mattan Hamou
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
group u list: _1201_
m user id: 0
owner id: 13899
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pelB leader sequence attached to enzyme LC-Cutinase protein coding sequence in order to allow the cell to bring the enzyme to the periplasm for easier secretion.
mutable notes: Adding sequence of pelB in front of sequence for Cuntinase
mutable provenance: http://aem.asm.org/content/78/5/1556.long Constructed from pelB leader sequence and protein coding sequence for LC-Cutinase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K936000
sequence annotation: BBa_J32015
sequence annotation: PelB
sequence: https://synbiohub.org/public/igem/BBa_K936013_sequence/1

BBa_K936000
type: SequenceAnnotation
persistent identity: annotation2178085
display id: annotation2178085
version: 1
top level: BBa_K936013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K936013/annotation2178085/range2178085/1

https://synbiohub.org/public/igem/BBa_K936013/annotation2178085/range2178085/1
type: Range
persistent identity: range2178085
display id: range2178085
version: 1
direction: direction/reverse
top level: BBa_K936013
start: 67
end: 951
orientation: inline

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation2178084
display id: annotation2178084
version: 1
top level: BBa_K936013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K936013/annotation2178084/range2178084/1

https://synbiohub.org/public/igem/BBa_K936013/annotation2178084/range2178084/1
type: Range
persistent identity: range2178084
display id: range2178084
version: 1
direction: direction/reverse
top level: BBa_K936013
start: 1
end: 66
orientation: inline

PelB
type: SequenceAnnotation
persistent identity: annotation2178083
display id: annotation2178083
version: 1
top level: BBa_K936013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K936013/annotation2178083/range2178083/1

https://synbiohub.org/public/igem/BBa_K936013/annotation2178083/range2178083/1
type: Range
persistent identity: range2178083
display id: range2178083
version: 1
direction: direction/forward
top level: BBa_K936013
start: 1
end: 66
orientation: inline

BBa_K763005
type: ComponentDefinition
persistent identity: BBa K763005
display id: BBa_K763005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pADH2 + Gene encoding YAP1 protein
creator: Luisa Mar??a Mart??nez S??nchez
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1014_
m user id: 0
owner id: 11712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: There are two transcriptional factors which regulates the ADH2 promoter: ADR1 a carbon source-responsibe zinc-finger2 and CAT83. In the absence of glucose both cis-acting sites in the promoter are bound cooperatively by the transcriptional activators. ADR1 binds to the UAS1 site while CAT8 binds to the UAS2/CSRE site and regulates positively the transcription of YAP1 protein by the activation of the ADH2 promoter. The presence of glucose downregulates the levels of the transcription factors (ADR1 and CAT8) which results in the depletion of the production of YAP1 protein by the repression of the ADH2 promoter.
mutable notes: No design considerations needed.
mutable provenance: The sequences of the promoter and the gene which encodes the YAP1 protein comes from yeast Sacharomyces cerevisiae genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K763005_sequence/1

tctA_505aa
type: ComponentDefinition
persistent identity: BBa K808004
display id: BBa_K808004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12829
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
mutable notes: deletion of PstI restriction site
mutable provenance: Comamonas testosteroni KF1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808004_sequence/1

BBa_K747015
type: ComponentDefinition
persistent identity: BBa K747015
display id: BBa_K747015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 1
sequence: https://synbiohub.org/public/igem/BBa_K747015_sequence/1

TT 1
type: SequenceAnnotation
persistent identity: annotation2195931
display id: annotation2195931
version: 1
top level: BBa_K747015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747015/annotation2195931/range2195931/1

https://synbiohub.org/public/igem/BBa_K747015/annotation2195931/range2195931/1
type: Range
persistent identity: range2195931
display id: range2195931
version: 1
direction: direction/forward
top level: BBa_K747015
start: 12
end: 209
orientation: inline

BBa_K567001
type: ComponentDefinition
persistent identity: BBa K567001
display id: BBa_K567001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI-Ptrc-tRNA(Arg)
creator: Chunying Li and Yunfeng Ruan
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The tRNA(Arg) is under the control of promoter tac. tRNA(Arg) expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.
mutable notes: The correct processing to produce mature tRNA should be ensured.
mutable provenance: LacI- Ptac derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: tRNA-Arg
sequence annotation: rrnB TE
sequence annotation: PlacI
sequence annotation: lacI
sequence annotation: Ptrc
sequence: https://synbiohub.org/public/igem/BBa_K567001_sequence/1

tRNA-Arg
type: SequenceAnnotation
persistent identity: annotation2152784
display id: annotation2152784
version: 1
top level: BBa_K567001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152784/range2152784/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152784/range2152784/1
type: Range
persistent identity: range2152784
display id: range2152784
version: 1
direction: direction/forward
top level: BBa_K567001
start: 1785
end: 1859
orientation: inline

rrnB TE
type: SequenceAnnotation
persistent identity: annotation2152785
display id: annotation2152785
version: 1
top level: BBa_K567001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152785/range2152785/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152785/range2152785/1
type: Range
persistent identity: range2152785
display id: range2152785
version: 1
direction: direction/forward
top level: BBa_K567001
start: 2054
end: 2133
orientation: inline

PlacI
type: SequenceAnnotation
persistent identity: annotation2152781
display id: annotation2152781
version: 1
top level: BBa_K567001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152781/range2152781/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152781/range2152781/1
type: Range
persistent identity: range2152781
display id: range2152781
version: 1
direction: direction/forward
top level: BBa_K567001
start: 331
end: 367
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2152782
display id: annotation2152782
version: 1
top level: BBa_K567001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152782/range2152782/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152782/range2152782/1
type: Range
persistent identity: range2152782
display id: range2152782
version: 1
direction: direction/forward
top level: BBa_K567001
start: 397
end: 1479
orientation: inline

Ptrc
type: SequenceAnnotation
persistent identity: annotation2152783
display id: annotation2152783
version: 1
top level: BBa_K567001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567001/annotation2152783/range2152783/1

https://synbiohub.org/public/igem/BBa_K567001/annotation2152783/range2152783/1
type: Range
persistent identity: range2152783
display id: range2152783
version: 1
direction: direction/forward
top level: BBa_K567001
start: 1709
end: 1739
orientation: inline

BBa_K786003
type: ComponentDefinition
persistent identity: BBa K786003
display id: BBa_K786003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRI fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: SRI-HtrI-Tar
sequence annotation: RBS 1.0
sequence: https://synbiohub.org/public/igem/BBa_K786003_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2199719
display id: annotation2199719
version: 1
top level: BBa_K786003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1
type: Range
persistent identity: range2199719
display id: range2199719
version: 1
direction: direction/forward
top level: BBa_K786003
start: 2029
end: 2158
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190185
display id: annotation2190185
version: 1
top level: BBa_K786003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1
type: Range
persistent identity: range2190185
display id: range2190185
version: 1
direction: direction/forward
top level: BBa_K786003
start: 1
end: 41
orientation: inline

SRI-HtrI-Tar
type: SequenceAnnotation
persistent identity: annotation2199706
display id: annotation2199706
version: 1
top level: BBa_K786003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1
type: Range
persistent identity: range2199706
display id: range2199706
version: 1
direction: direction/forward
top level: BBa_K786003
start: 68
end: 1992
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190186
display id: annotation2190186
version: 1
top level: BBa_K786003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1
type: Range
persistent identity: range2190186
display id: range2190186
version: 1
direction: direction/forward
top level: BBa_K786003
start: 42
end: 61
orientation: inline

BBa_K864680
type: ComponentDefinition
persistent identity: BBa K864680
display id: BBa_K864680
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetM
creator: Erik Lundin
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: tetM
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetM
sequence: https://synbiohub.org/public/igem/BBa_K864680_sequence/1

tetM
type: SequenceAnnotation
persistent identity: annotation2214190
display id: annotation2214190
version: 1
top level: BBa_K864680
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864680/annotation2214190/range2214190/1

https://synbiohub.org/public/igem/BBa_K864680/annotation2214190/range2214190/1
type: Range
persistent identity: range2214190
display id: range2214190
version: 1
direction: direction/forward
top level: BBa_K864680
start: 1
end: 1920
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lacI regulated, lambda pL hybrid)
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
direction: direction/forward
top level: lacI+pL
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
direction: direction/forward
top level: lacI+pL
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
direction: direction/forward
top level: lacI+pL
start: 43
end: 48
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
direction: direction/forward
top level: lacI+pL
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
direction: direction/forward
top level: lacI+pL
start: 26
end: 42
orientation: inline

BBa_S05051
type: ComponentDefinition
persistent identity: BBa S05051
display id: BBa_S05051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:K748003
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:14:49Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_K748003
sequence annotation: BBa_K748003
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S05051_sequence/1

BBa_B0034
type: Component
persistent identity: component2183421
display id: component2183421
version: 1
top level: BBa_S05051
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K748003
type: Component
persistent identity: component2183422
display id: component2183422
version: 1
top level: BBa_S05051
access: public
definition: BBa_K748003

BBa_K748003
type: SequenceAnnotation
persistent identity: annotation2183422
display id: annotation2183422
version: 1
top level: BBa_S05051
location: https://synbiohub.org/public/igem/BBa_S05051/annotation2183422/range2183422/1
component: BBa_K748003

https://synbiohub.org/public/igem/BBa_S05051/annotation2183422/range2183422/1
type: Range
persistent identity: range2183422
display id: range2183422
version: 1
direction: direction/reverse
top level: BBa_S05051
start: 19
end: 1404
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2183421
display id: annotation2183421
version: 1
top level: BBa_S05051
location: https://synbiohub.org/public/igem/BBa_S05051/annotation2183421/range2183421/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05051/annotation2183421/range2183421/1
type: Range
persistent identity: range2183421
display id: range2183421
version: 1
direction: direction/reverse
top level: BBa_S05051
start: 1
end: 12
orientation: inline

BBa_K844006
type: ComponentDefinition
persistent identity: BBa K844006
display id: BBa_K844006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x Subunit "W" with Met (ATG) start codon
creator: Federico Carlos Rodriguez
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
group u list: _1104_
m user id: 0
owner id: 13908
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spider silk subunit native sequence; contains two elasticity domains and one strength domain, contains added Met (ATG) on 5??? end.
mutable notes: none
mutable provenance: Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Beta-spiral
sequence annotation: Elasticity Domain
sequence annotation: Beta-helix
sequence annotation: Linker Domain
sequence annotation: Strength Domain
sequence: https://synbiohub.org/public/igem/BBa_K844006_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2212385
display id: annotation2212385
version: 1
top level: BBa_K844006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212385/range2212385/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212385/range2212385/1
type: Range
persistent identity: range2212385
display id: range2212385
version: 1
direction: direction/forward
top level: BBa_K844006
start: 1
end: 3
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212388
display id: annotation2212388
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212388/range2212388/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212388/range2212388/1
type: Range
persistent identity: range2212388
display id: range2212388
version: 1
direction: direction/forward
top level: BBa_K844006
start: 13
end: 27
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212386
display id: annotation2212386
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212386/range2212386/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212386/range2212386/1
type: Range
persistent identity: range2212386
display id: range2212386
version: 1
direction: direction/forward
top level: BBa_K844006
start: 4
end: 87
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212395
display id: annotation2212395
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212395/range2212395/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212395/range2212395/1
type: Range
persistent identity: range2212395
display id: range2212395
version: 1
direction: direction/forward
top level: BBa_K844006
start: 117
end: 132
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212393
display id: annotation2212393
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212393/range2212393/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212393/range2212393/1
type: Range
persistent identity: range2212393
display id: range2212393
version: 1
direction: direction/forward
top level: BBa_K844006
start: 88
end: 171
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212389
display id: annotation2212389
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212389/range2212389/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212389/range2212389/1
type: Range
persistent identity: range2212389
display id: range2212389
version: 1
direction: direction/forward
top level: BBa_K844006
start: 34
end: 48
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212387
display id: annotation2212387
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212387/range2212387/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212387/range2212387/1
type: Range
persistent identity: range2212387
display id: range2212387
version: 1
direction: direction/forward
top level: BBa_K844006
start: 4
end: 12
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212398
display id: annotation2212398
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212398/range2212398/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212398/range2212398/1
type: Range
persistent identity: range2212398
display id: range2212398
version: 1
direction: direction/forward
top level: BBa_K844006
start: 172
end: 188
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212390
display id: annotation2212390
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212390/range2212390/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212390/range2212390/1
type: Range
persistent identity: range2212390
display id: range2212390
version: 1
direction: direction/forward
top level: BBa_K844006
start: 49
end: 63
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212394
display id: annotation2212394
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212394/range2212394/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212394/range2212394/1
type: Range
persistent identity: range2212394
display id: range2212394
version: 1
direction: direction/forward
top level: BBa_K844006
start: 98
end: 113
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212392
display id: annotation2212392
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212392/range2212392/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212392/range2212392/1
type: Range
persistent identity: range2212392
display id: range2212392
version: 1
direction: direction/forward
top level: BBa_K844006
start: 88
end: 97
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212397
display id: annotation2212397
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212397/range2212397/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212397/range2212397/1
type: Range
persistent identity: range2212397
display id: range2212397
version: 1
direction: direction/forward
top level: BBa_K844006
start: 156
end: 171
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212391
display id: annotation2212391
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212391/range2212391/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212391/range2212391/1
type: Range
persistent identity: range2212391
display id: range2212391
version: 1
direction: direction/forward
top level: BBa_K844006
start: 72
end: 87
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212399
display id: annotation2212399
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212399/range2212399/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212399/range2212399/1
type: Range
persistent identity: range2212399
display id: range2212399
version: 1
direction: direction/forward
top level: BBa_K844006
start: 189
end: 207
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212396
display id: annotation2212396
version: 1
top level: BBa_K844006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844006/annotation2212396/range2212396/1

https://synbiohub.org/public/igem/BBa_K844006/annotation2212396/range2212396/1
type: Range
persistent identity: range2212396
display id: range2212396
version: 1
direction: direction/forward
top level: BBa_K844006
start: 133
end: 147
orientation: inline

BBa_K747084
type: ComponentDefinition
persistent identity: BBa K747084
display id: BBa_K747084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CA6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CA 6
sequence: https://synbiohub.org/public/igem/BBa_K747084_sequence/1

CA 6
type: SequenceAnnotation
persistent identity: annotation2197057
display id: annotation2197057
version: 1
top level: BBa_K747084
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747084/annotation2197057/range2197057/1

https://synbiohub.org/public/igem/BBa_K747084/annotation2197057/range2197057/1
type: Range
persistent identity: range2197057
display id: range2197057
version: 1
direction: direction/forward
top level: BBa_K747084
start: 14
end: 211
orientation: inline

BBa_K936000
type: ComponentDefinition
persistent identity: BBa K936000
display id: BBa_K936000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LC Cutinase
creator: Nicholas Csicsery
created: 2012-07-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
group u list: _1201_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the LC Cutinase gene used to break PET into terephthalic acid and ethylene glycol.
mutable notes: Eliminated BioBrick restriction sites and added a TAA double stop.
mutable provenance: Modified from the paper Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach by Sulaiman et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K936000_sequence/1

BBa_K909007
type: ComponentDefinition
persistent identity: BBa K909007
display id: BBa_K909007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR DNA binding domain containing BamHI site for protein fusions
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Truncated version of tetracycline repressor TetR, consisting of 1 ??? 127 amino acids, containing DNA binding domain. The rest of the structure, 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression. In addition, we introduced BamHI site at C terminus for an easy in tetR-DBD frame fusions with other proteins. Thus, it can be used in two hybrid systems for both, homo and hetero dimerizations, also one can use these fusions to turn protein-protein interaction into the repression of transcription.
mutable notes: Deletion of 8-10 helixes might lower the stability of protein, also TetR-DBD is no longer responsive to anhydrotetracycline
mutable provenance: E. coli Tn10 transposon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: BamHI restriction site
sequence: https://synbiohub.org/public/igem/BBa_K909007_sequence/1

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2194604
display id: annotation2194604
version: 1
top level: BBa_K909007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909007/annotation2194604/range2194604/1

https://synbiohub.org/public/igem/BBa_K909007/annotation2194604/range2194604/1
type: Range
persistent identity: range2194604
display id: range2194604
version: 1
direction: direction/forward
top level: BBa_K909007
start: 382
end: 387
orientation: inline

BBa_K747067
type: ComponentDefinition
persistent identity: BBa K747067
display id: BBa_K747067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 5
sequence: https://synbiohub.org/public/igem/BBa_K747067_sequence/1

AT 5
type: SequenceAnnotation
persistent identity: annotation2197030
display id: annotation2197030
version: 1
top level: BBa_K747067
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747067/annotation2197030/range2197030/1

https://synbiohub.org/public/igem/BBa_K747067/annotation2197030/range2197030/1
type: Range
persistent identity: range2197030
display id: range2197030
version: 1
direction: direction/forward
top level: BBa_K747067
start: 14
end: 211
orientation: inline

BBa_J202003
type: ComponentDefinition
persistent identity: BBa J202003
display id: BBa_J202003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#4
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -63(T to C), -42 (C to A), and +9 (T to A) relative to the araC transcriptional start site were changed.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by random mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation 2
sequence annotation: Mutation 3
sequence annotation: araI2
sequence annotation: Transcription Start
sequence annotation: Mutation 1
sequence annotation: araI1
sequence: https://synbiohub.org/public/igem/BBa_J202003_sequence/1

Mutation 2
type: SequenceAnnotation
persistent identity: annotation2178430
display id: annotation2178430
version: 1
top level: BBa_J202003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178430/range2178430/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178430/range2178430/1
type: Range
persistent identity: range2178430
display id: range2178430
version: 1
direction: direction/forward
top level: BBa_J202003
start: 70
end: 70
orientation: inline

Mutation 3
type: SequenceAnnotation
persistent identity: annotation2178431
display id: annotation2178431
version: 1
top level: BBa_J202003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178431/range2178431/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178431/range2178431/1
type: Range
persistent identity: range2178431
display id: range2178431
version: 1
direction: direction/forward
top level: BBa_J202003
start: 120
end: 120
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178428
display id: annotation2178428
version: 1
top level: BBa_J202003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178428/range2178428/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178428/range2178428/1
type: Range
persistent identity: range2178428
display id: range2178428
version: 1
direction: direction/forward
top level: BBa_J202003
start: 61
end: 77
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178426
display id: annotation2178426
version: 1
top level: BBa_J202003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178426/range2178426/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178426/range2178426/1
type: Range
persistent identity: range2178426
display id: range2178426
version: 1
direction: direction/forward
top level: BBa_J202003
start: 112
end: 112
orientation: inline

Mutation 1
type: SequenceAnnotation
persistent identity: annotation2178429
display id: annotation2178429
version: 1
top level: BBa_J202003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178429/range2178429/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178429/range2178429/1
type: Range
persistent identity: range2178429
display id: range2178429
version: 1
direction: direction/forward
top level: BBa_J202003
start: 49
end: 49
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178427
display id: annotation2178427
version: 1
top level: BBa_J202003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202003/annotation2178427/range2178427/1

https://synbiohub.org/public/igem/BBa_J202003/annotation2178427/range2178427/1
type: Range
persistent identity: range2178427
display id: range2178427
version: 1
direction: direction/forward
top level: BBa_J202003
start: 40
end: 56
orientation: inline

BBa_K747075
type: ComponentDefinition
persistent identity: BBa K747075
display id: BBa_K747075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 5
sequence: https://synbiohub.org/public/igem/BBa_K747075_sequence/1

GT 5
type: SequenceAnnotation
persistent identity: annotation2197044
display id: annotation2197044
version: 1
top level: BBa_K747075
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747075/annotation2197044/range2197044/1

https://synbiohub.org/public/igem/BBa_K747075/annotation2197044/range2197044/1
type: Range
persistent identity: range2197044
display id: range2197044
version: 1
direction: direction/forward
top level: BBa_K747075
start: 14
end: 211
orientation: inline

BBa_K747091
type: ComponentDefinition
persistent identity: BBa K747091
display id: BBa_K747091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 6
sequence: https://synbiohub.org/public/igem/BBa_K747091_sequence/1

GT 6
type: SequenceAnnotation
persistent identity: annotation2197069
display id: annotation2197069
version: 1
top level: BBa_K747091
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747091/annotation2197069/range2197069/1

https://synbiohub.org/public/igem/BBa_K747091/annotation2197069/range2197069/1
type: Range
persistent identity: range2197069
display id: range2197069
version: 1
direction: direction/forward
top level: BBa_K747091
start: 14
end: 211
orientation: inline

BBa_K747054
type: ComponentDefinition
persistent identity: BBa K747054
display id: BBa_K747054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 4
sequence: https://synbiohub.org/public/igem/BBa_K747054_sequence/1

CG 4
type: SequenceAnnotation
persistent identity: annotation2196846
display id: annotation2196846
version: 1
top level: BBa_K747054
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747054/annotation2196846/range2196846/1

https://synbiohub.org/public/igem/BBa_K747054/annotation2196846/range2196846/1
type: Range
persistent identity: range2196846
display id: range2196846
version: 1
direction: direction/forward
top level: BBa_K747054
start: 14
end: 211
orientation: inline

BBa_I13013
type: ComponentDefinition
persistent identity: BBa I13013
display id: BBa_I13013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0032.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0040
component: BBa_B0032
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13013_sequence/1

BBa_R0040
type: Component
persistent identity: component946842
display id: component946842
version: 1
top level: BBa_I13013
access: public
definition: p(tetR)

BBa_B0032
type: Component
persistent identity: component946853
display id: component946853
version: 1
top level: BBa_I13013
access: public
definition: BBa_B0032

BBa_B0010
type: Component
persistent identity: component946861
display id: component946861
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component946871
display id: component946871
version: 1
top level: BBa_I13013
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component946856
display id: component946856
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation946871
display id: annotation946871
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946871/range946871/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13013/annotation946871/range946871/1
type: Range
persistent identity: range946871
display id: range946871
version: 1
direction: direction/forward
top level: BBa_I13013
start: 901
end: 941
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation946842
display id: annotation946842
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946842/range946842/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13013/annotation946842/range946842/1
type: Range
persistent identity: range946842
display id: range946842
version: 1
direction: direction/forward
top level: BBa_I13013
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation946853
display id: annotation946853
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946853/range946853/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13013/annotation946853/range946853/1
type: Range
persistent identity: range946853
display id: range946853
version: 1
direction: direction/forward
top level: BBa_I13013
start: 63
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation946861
display id: annotation946861
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946861/range946861/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13013/annotation946861/range946861/1
type: Range
persistent identity: range946861
display id: range946861
version: 1
direction: direction/forward
top level: BBa_I13013
start: 813
end: 892
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation946856
display id: annotation946856
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946856/range946856/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13013/annotation946856/range946856/1
type: Range
persistent identity: range946856
display id: range946856
version: 1
direction: direction/forward
top level: BBa_I13013
start: 82
end: 804
orientation: inline

BBa_K747000
type: ComponentDefinition
persistent identity: BBa K747000
display id: BBa_K747000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA1_DiRepeat
creator: Lucas Schneider
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Part specifies the DNA binding affinity of the 2. and 3. base pair of a Transactivator-like (TAL) protein. The TAL-Protein binds a specific fourteen base pair DNA sequence. The 2. and 3. base pair of this part is specified by adenine-adenine.
mutable notes: This part can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg.
mutable provenance: This Part was synthesized and flanked with BsmB1-restriction sites
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 1
sequence: https://synbiohub.org/public/igem/BBa_K747000_sequence/1

AA 1
type: SequenceAnnotation
persistent identity: annotation2195665
display id: annotation2195665
version: 1
top level: BBa_K747000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747000/annotation2195665/range2195665/1

https://synbiohub.org/public/igem/BBa_K747000/annotation2195665/range2195665/1
type: Range
persistent identity: range2195665
display id: range2195665
version: 1
direction: direction/forward
top level: BBa_K747000
start: 12
end: 209
orientation: inline

BBa_K567008
type: ComponentDefinition
persistent identity: BBa K567008
display id: BBa_K567008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-Luc-2AGG
creator: Yunfeng Ruan and Chunying Li
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 2 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg) (BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: PCR is used to insert the 2 AGG codons into the gene after ATG.
mutable provenance: T7 promoter is derived from pET28A. Wild type Luciferase from BBa_I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lac operator
sequence annotation: luciferase
sequence annotation: ATG
sequence annotation: rbs
sequence annotation: 2AGG
sequence annotation: PT7
sequence: https://synbiohub.org/public/igem/BBa_K567008_sequence/1

lac operator
type: SequenceAnnotation
persistent identity: annotation2150785
display id: annotation2150785
version: 1
top level: BBa_K567008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150785/range2150785/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150785/range2150785/1
type: Range
persistent identity: range2150785
display id: range2150785
version: 1
direction: direction/forward
top level: BBa_K567008
start: 21
end: 44
orientation: inline

luciferase
type: SequenceAnnotation
persistent identity: annotation2150771
display id: annotation2150771
version: 1
top level: BBa_K567008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150771/range2150771/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150771/range2150771/1
type: Range
persistent identity: range2150771
display id: range2150771
version: 1
direction: direction/forward
top level: BBa_K567008
start: 89
end: 1756
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150769
display id: annotation2150769
version: 1
top level: BBa_K567008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150769/range2150769/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150769/range2150769/1
type: Range
persistent identity: range2150769
display id: range2150769
version: 1
direction: direction/forward
top level: BBa_K567008
start: 89
end: 91
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150787
display id: annotation2150787
version: 1
top level: BBa_K567008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150787/range2150787/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150787/range2150787/1
type: Range
persistent identity: range2150787
display id: range2150787
version: 1
direction: direction/forward
top level: BBa_K567008
start: 75
end: 81
orientation: inline

2AGG
type: SequenceAnnotation
persistent identity: annotation2150770
display id: annotation2150770
version: 1
top level: BBa_K567008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150770/range2150770/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150770/range2150770/1
type: Range
persistent identity: range2150770
display id: range2150770
version: 1
direction: direction/forward
top level: BBa_K567008
start: 95
end: 100
orientation: inline

PT7
type: SequenceAnnotation
persistent identity: annotation2150784
display id: annotation2150784
version: 1
top level: BBa_K567008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150784/range2150784/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150784/range2150784/1
type: Range
persistent identity: range2150784
display id: range2150784
version: 1
direction: direction/forward
top level: BBa_K567008
start: 2
end: 18
orientation: inline

BBa_K747041
type: ComponentDefinition
persistent identity: BBa K747041
display id: BBa_K747041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 3
sequence: https://synbiohub.org/public/igem/BBa_K747041_sequence/1

GC 3
type: SequenceAnnotation
persistent identity: annotation2196252
display id: annotation2196252
version: 1
top level: BBa_K747041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747041/annotation2196252/range2196252/1

https://synbiohub.org/public/igem/BBa_K747041/annotation2196252/range2196252/1
type: Range
persistent identity: range2196252
display id: range2196252
version: 1
direction: direction/forward
top level: BBa_K747041
start: 14
end: 211
orientation: inline

BBa_K876004
type: ComponentDefinition
persistent identity: BBa K876004
display id: BBa_K876004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-RBS-GFP-T-pLac-TetR
creator: Alec Spinhirne
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1138_
m user id: 0
owner id: 13834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Test mechanism for the effectiveness of TetR in repressing pTet. Also used to show the effect of ATc on TetR
mutable notes: Biobrick placement
mutable provenance: Created from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_C0040
component: BBa_K081012
component: BBa_R0040
sequence annotation: BBa_C0040
sequence annotation: BBa_K081012
sequence annotation: BBa_R0010
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K876004_sequence/1

BBa_R0010
type: Component
persistent identity: component2264641
display id: component2264641
version: 1
top level: BBa_K876004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0040
type: Component
persistent identity: component2264651
display id: component2264651
version: 1
top level: BBa_K876004
access: public
definition: tetR

BBa_K081012
type: Component
persistent identity: component2264640
display id: component2264640
version: 1
top level: BBa_K876004
access: public
definition: https://synbiohub.org/public/igem/BBa_K081012/1

BBa_R0040
type: Component
persistent identity: component2264625
display id: component2264625
version: 1
top level: BBa_K876004
access: public
definition: p(tetR)

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2264651
display id: annotation2264651
version: 1
top level: BBa_K876004
location: https://synbiohub.org/public/igem/BBa_K876004/annotation2264651/range2264651/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876004/annotation2264651/range2264651/1
type: Range
persistent identity: range2264651
display id: range2264651
version: 1
direction: direction/reverse
top level: BBa_K876004
start: 1065
end: 1749
orientation: inline

BBa_K081012
type: SequenceAnnotation
persistent identity: annotation2264640
display id: annotation2264640
version: 1
top level: BBa_K876004
location: https://synbiohub.org/public/igem/BBa_K876004/annotation2264640/range2264640/1
component: BBa_K081012

https://synbiohub.org/public/igem/BBa_K876004/annotation2264640/range2264640/1
type: Range
persistent identity: range2264640
display id: range2264640
version: 1
direction: direction/reverse
top level: BBa_K876004
start: 63
end: 850
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2264641
display id: annotation2264641
version: 1
top level: BBa_K876004
location: https://synbiohub.org/public/igem/BBa_K876004/annotation2264641/range2264641/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876004/annotation2264641/range2264641/1
type: Range
persistent identity: range2264641
display id: range2264641
version: 1
direction: direction/forward
top level: BBa_K876004
start: 859
end: 1058
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2264625
display id: annotation2264625
version: 1
top level: BBa_K876004
location: https://synbiohub.org/public/igem/BBa_K876004/annotation2264625/range2264625/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876004/annotation2264625/range2264625/1
type: Range
persistent identity: range2264625
display id: range2264625
version: 1
direction: direction/forward
top level: BBa_K876004
start: 1
end: 54
orientation: inline

BBa_K747028
type: ComponentDefinition
persistent identity: BBa K747028
display id: BBa_K747028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 2
sequence: https://synbiohub.org/public/igem/BBa_K747028_sequence/1

TA 2
type: SequenceAnnotation
persistent identity: annotation2196199
display id: annotation2196199
version: 1
top level: BBa_K747028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747028/annotation2196199/range2196199/1

https://synbiohub.org/public/igem/BBa_K747028/annotation2196199/range2196199/1
type: Range
persistent identity: range2196199
display id: range2196199
version: 1
direction: direction/forward
top level: BBa_K747028
start: 14
end: 211
orientation: inline

BBa_J202009
type: ComponentDefinition
persistent identity: BBa J202009
display id: BBa_J202009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#10
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -59 relative to the araC transcriptional start site was changed from C to T. Also, C at -48 was changed to T.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by random mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation 1
sequence annotation: Transcription Start
sequence annotation: Mutation 2
sequence annotation: araI2
sequence annotation: araI1
sequence: https://synbiohub.org/public/igem/BBa_J202009_sequence/1

Mutation 1
type: SequenceAnnotation
persistent identity: annotation2178452
display id: annotation2178452
version: 1
top level: BBa_J202009
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178452/range2178452/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178452/range2178452/1
type: Range
persistent identity: range2178452
display id: range2178452
version: 1
direction: direction/forward
top level: BBa_J202009
start: 53
end: 53
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178454
display id: annotation2178454
version: 1
top level: BBa_J202009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178454/range2178454/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178454/range2178454/1
type: Range
persistent identity: range2178454
display id: range2178454
version: 1
direction: direction/forward
top level: BBa_J202009
start: 112
end: 112
orientation: inline

Mutation 2
type: SequenceAnnotation
persistent identity: annotation2178453
display id: annotation2178453
version: 1
top level: BBa_J202009
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178453/range2178453/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178453/range2178453/1
type: Range
persistent identity: range2178453
display id: range2178453
version: 1
direction: direction/forward
top level: BBa_J202009
start: 64
end: 64
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178456
display id: annotation2178456
version: 1
top level: BBa_J202009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178456/range2178456/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178456/range2178456/1
type: Range
persistent identity: range2178456
display id: range2178456
version: 1
direction: direction/forward
top level: BBa_J202009
start: 61
end: 77
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178455
display id: annotation2178455
version: 1
top level: BBa_J202009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202009/annotation2178455/range2178455/1

https://synbiohub.org/public/igem/BBa_J202009/annotation2178455/range2178455/1
type: Range
persistent identity: range2178455
display id: range2178455
version: 1
direction: direction/forward
top level: BBa_J202009
start: 40
end: 56
orientation: inline

BBa_K876000
type: ComponentDefinition
persistent identity: BBa K876000
display id: BBa_K876000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-TetR
creator: Alec Spinhirne
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive producer for TetR, repressed by LacI
mutable notes: Biobrick placement
mutable provenance: Created from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_C0040
sequence annotation: BBa_R0010
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K876000_sequence/1

BBa_R0010
type: Component
persistent identity: component2245076
display id: component2245076
version: 1
top level: BBa_K876000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0040
type: Component
persistent identity: component2245086
display id: component2245086
version: 1
top level: BBa_K876000
access: public
definition: tetR

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2245076
display id: annotation2245076
version: 1
top level: BBa_K876000
location: https://synbiohub.org/public/igem/BBa_K876000/annotation2245076/range2245076/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876000/annotation2245076/range2245076/1
type: Range
persistent identity: range2245076
display id: range2245076
version: 1
direction: direction/forward
top level: BBa_K876000
start: 1
end: 200
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2245086
display id: annotation2245086
version: 1
top level: BBa_K876000
location: https://synbiohub.org/public/igem/BBa_K876000/annotation2245086/range2245086/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876000/annotation2245086/range2245086/1
type: Range
persistent identity: range2245086
display id: range2245086
version: 1
direction: direction/reverse
top level: BBa_K876000
start: 207
end: 891
orientation: inline

BBa_K747005
type: ComponentDefinition
persistent identity: BBa K747005
display id: BBa_K747005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cgtctcaTGACcccggaacaggtggtggccattgcatctcacgatggtggtAagcaggccttggagaccgttcagcgcCt gCtgccagtgCtgtgtcaggcccatggcctcacaccggaacaggtggtggccatcgccagccacgacggcggcaaacagg ctCtggaaactgtgcagcgcCtgttgccggtgCtgtgtcaggcccacggGCTCtgagacg
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 1
sequence: https://synbiohub.org/public/igem/BBa_K747005_sequence/1

CC 1
type: SequenceAnnotation
persistent identity: annotation2195792
display id: annotation2195792
version: 1
top level: BBa_K747005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747005/annotation2195792/range2195792/1

https://synbiohub.org/public/igem/BBa_K747005/annotation2195792/range2195792/1
type: Range
persistent identity: range2195792
display id: range2195792
version: 1
direction: direction/forward
top level: BBa_K747005
start: 12
end: 209
orientation: inline

BBa_B0023
type: ComponentDefinition
persistent identity: BBa B0023
display id: BBa_B0023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphage T7, reversed
creator: Caitlin Conboy
created: 2003-10-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the reverse of terminator B0013, a bacteriophage T7 terminator which was engineered to be bidirectional. Contains a poly A insertion upstream of a 20 bp stem-loop.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0023_sequence/1

BBa_K747019
type: ComponentDefinition
persistent identity: BBa K747019
display id: BBa_K747019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 2
sequence: https://synbiohub.org/public/igem/BBa_K747019_sequence/1

AT 2
type: SequenceAnnotation
persistent identity: annotation2195984
display id: annotation2195984
version: 1
top level: BBa_K747019
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747019/annotation2195984/range2195984/1

https://synbiohub.org/public/igem/BBa_K747019/annotation2195984/range2195984/1
type: Range
persistent identity: range2195984
display id: range2195984
version: 1
direction: direction/forward
top level: BBa_K747019
start: 14
end: 211
orientation: inline

BBa_I6080
type: ComponentDefinition
persistent identity: BBa I6080
display id: BBa_I6080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1051.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-17T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_R1051
component: BBa_B0010
component: BBa_B0034
component: BBa_E0032
sequence annotation: BBa_B0034
sequence annotation: BBa_R1051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I6080_sequence/1

BBa_B0012
type: Component
persistent identity: component949476
display id: component949476
version: 1
top level: BBa_I6080
access: public
definition: BBa_B0012

BBa_R1051
type: Component
persistent identity: component949438
display id: component949438
version: 1
top level: BBa_I6080
access: public
definition: https://synbiohub.org/public/igem/BBa_R1051/1

BBa_B0010
type: Component
persistent identity: component949466
display id: component949466
version: 1
top level: BBa_I6080
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component949446
display id: component949446
version: 1
top level: BBa_I6080
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component949458
display id: component949458
version: 1
top level: BBa_I6080
access: public
definition: YFP

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949446
display id: annotation949446
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949446/range949446/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6080/annotation949446/range949446/1
type: Range
persistent identity: range949446
display id: range949446
version: 1
direction: direction/forward
top level: BBa_I6080
start: 58
end: 69
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation949438
display id: annotation949438
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949438/range949438/1
component: BBa_R1051

https://synbiohub.org/public/igem/BBa_I6080/annotation949438/range949438/1
type: Range
persistent identity: range949438
display id: range949438
version: 1
direction: direction/forward
top level: BBa_I6080
start: 1
end: 49
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949466
display id: annotation949466
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949466/range949466/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6080/annotation949466/range949466/1
type: Range
persistent identity: range949466
display id: range949466
version: 1
direction: direction/forward
top level: BBa_I6080
start: 846
end: 925
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949476
display id: annotation949476
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949476/range949476/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6080/annotation949476/range949476/1
type: Range
persistent identity: range949476
display id: range949476
version: 1
direction: direction/forward
top level: BBa_I6080
start: 934
end: 974
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949458
display id: annotation949458
version: 1
top level: BBa_I6080
location: https://synbiohub.org/public/igem/BBa_I6080/annotation949458/range949458/1
component: BBa_E0032

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type: Range
persistent identity: range949458
display id: range949458
version: 1
direction: direction/forward
top level: BBa_I6080
start: 76
end: 837
orientation: inline

BBa_K737051
type: ComponentDefinition
persistent identity: BBa K737051
display id: BBa_K737051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible GFP Generator
creator: Weihong Lai and Peiran Zhang
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [J23106,P0412]is a constitutive lacI repressor generator,Fluorescence can be observed with induced by IPTG. The RBS of E0240 is B0030,a weak GFP translational unit specialized to quantitively describe the behaviour of promotor in many cases. NOTE: R0011 don???t work well in high copy number plasmids.
mutable notes: No
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23106
component: BBa_E0240
component: BBa_R0011
component: BBa_P0412
sequence annotation: BBa_R0011
sequence annotation: BBa_P0412
sequence annotation: BBa_J23106
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K737051_sequence/1

BBa_J23106
type: Component
persistent identity: component2185885
display id: component2185885
version: 1
top level: BBa_K737051
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_E0240
type: Component
persistent identity: component2185914
display id: component2185914
version: 1
top level: BBa_K737051
access: public
definition: GFP report

BBa_R0011
type: Component
persistent identity: component2185899
display id: component2185899
version: 1
top level: BBa_K737051
access: public
definition: lacI+pL

BBa_P0412
type: Component
persistent identity: component2185898
display id: component2185898
version: 1
top level: BBa_K737051
access: public
definition: https://synbiohub.org/public/igem/BBa_P0412/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2185899
display id: annotation2185899
version: 1
top level: BBa_K737051
location: https://synbiohub.org/public/igem/BBa_K737051/annotation2185899/range2185899/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K737051/annotation2185899/range2185899/1
type: Range
persistent identity: range2185899
display id: range2185899
version: 1
direction: direction/forward
top level: BBa_K737051
start: 1360
end: 1413
orientation: inline

BBa_P0412
type: SequenceAnnotation
persistent identity: annotation2185898
display id: annotation2185898
version: 1
top level: BBa_K737051
location: https://synbiohub.org/public/igem/BBa_K737051/annotation2185898/range2185898/1
component: BBa_P0412

https://synbiohub.org/public/igem/BBa_K737051/annotation2185898/range2185898/1
type: Range
persistent identity: range2185898
display id: range2185898
version: 1
direction: direction/reverse
top level: BBa_K737051
start: 44
end: 1351
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2185885
display id: annotation2185885
version: 1
top level: BBa_K737051
location: https://synbiohub.org/public/igem/BBa_K737051/annotation2185885/range2185885/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K737051/annotation2185885/range2185885/1
type: Range
persistent identity: range2185885
display id: range2185885
version: 1
direction: direction/reverse
top level: BBa_K737051
start: 1
end: 35
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2185914
display id: annotation2185914
version: 1
top level: BBa_K737051
location: https://synbiohub.org/public/igem/BBa_K737051/annotation2185914/range2185914/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K737051/annotation2185914/range2185914/1
type: Range
persistent identity: range2185914
display id: range2185914
version: 1
direction: direction/reverse
top level: BBa_K737051
start: 1423
end: 2298
orientation: inline

BBa_S0110
type: ComponentDefinition
persistent identity: BBa S0110
display id: BBa_S0110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0033.C0052
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0052
component: BBa_B0033
sequence annotation: BBa_C0052
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_S0110_sequence/1

BBa_C0052
type: Component
persistent identity: component939884
display id: component939884
version: 1
top level: BBa_S0110
access: public
definition: cI 434

BBa_B0033
type: Component
persistent identity: component939873
display id: component939873
version: 1
top level: BBa_S0110
access: public
definition: BBa_B0033

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation939884
display id: annotation939884
version: 1
top level: BBa_S0110
location: https://synbiohub.org/public/igem/BBa_S0110/annotation939884/range939884/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_S0110/annotation939884/range939884/1
type: Range
persistent identity: range939884
display id: range939884
version: 1
direction: direction/forward
top level: BBa_S0110
start: 18
end: 686
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939873
display id: annotation939873
version: 1
top level: BBa_S0110
location: https://synbiohub.org/public/igem/BBa_S0110/annotation939873/range939873/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_S0110/annotation939873/range939873/1
type: Range
persistent identity: range939873
display id: range939873
version: 1
direction: direction/forward
top level: BBa_S0110
start: 1
end: 11
orientation: inline

BBa_K876011
type: ComponentDefinition
persistent identity: BBa K876011
display id: BBa_K876011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Population 2 for use with the dual strain toggle
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_C0012
component: BBa_C0079
component: BBa_B0015
component: BBa_C0061
component: BBa_K081014
component: BBa_R0010
component: BBa_R0079
sequence annotation: BBa_B0015
sequence annotation: BBa_C0012
sequence annotation: BBa_C0061
sequence annotation: BBa_C0079
sequence annotation: BBa_R0010
sequence annotation: BBa_R0040
sequence annotation: BBa_R0079
sequence annotation: BBa_K081014
sequence: https://synbiohub.org/public/igem/BBa_K876011_sequence/1

BBa_R0040
type: Component
persistent identity: component2295828
display id: component2295828
version: 1
top level: BBa_K876011
access: public
definition: p(tetR)

BBa_C0012
type: Component
persistent identity: component2295851
display id: component2295851
version: 1
top level: BBa_K876011
access: public
definition: lacI

BBa_C0079
type: Component
persistent identity: component2295837
display id: component2295837
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_B0015
type: Component
persistent identity: component2295844
display id: component2295844
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0061
type: Component
persistent identity: component2295874
display id: component2295874
version: 1
top level: BBa_K876011
access: public
definition: luxI

BBa_K081014
type: Component
persistent identity: component2295865
display id: component2295865
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_K081014/1

BBa_R0010
type: Component
persistent identity: component2295866
display id: component2295866
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0079
type: Component
persistent identity: component2295849
display id: component2295849
version: 1
top level: BBa_K876011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2295844
display id: annotation2295844
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295844/range2295844/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876011/annotation2295844/range2295844/1
type: Range
persistent identity: range2295844
display id: range2295844
version: 1
direction: direction/reverse
top level: BBa_K876011
start: 850
end: 978
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2295851
display id: annotation2295851
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295851/range2295851/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K876011/annotation2295851/range2295851/1
type: Range
persistent identity: range2295851
display id: range2295851
version: 1
direction: direction/forward
top level: BBa_K876011
start: 1150
end: 2277
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2295874
display id: annotation2295874
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295874/range2295874/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876011/annotation2295874/range2295874/1
type: Range
persistent identity: range2295874
display id: range2295874
version: 1
direction: direction/forward
top level: BBa_K876011
start: 3299
end: 3916
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2295837
display id: annotation2295837
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295837/range2295837/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K876011/annotation2295837/range2295837/1
type: Range
persistent identity: range2295837
display id: range2295837
version: 1
direction: direction/reverse
top level: BBa_K876011
start: 61
end: 841
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2295866
display id: annotation2295866
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295866/range2295866/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876011/annotation2295866/range2295866/1
type: Range
persistent identity: range2295866
display id: range2295866
version: 1
direction: direction/forward
top level: BBa_K876011
start: 3093
end: 3292
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2295828
display id: annotation2295828
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295828/range2295828/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K876011/annotation2295828/range2295828/1
type: Range
persistent identity: range2295828
display id: range2295828
version: 1
direction: direction/forward
top level: BBa_K876011
start: 1
end: 54
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2295849
display id: annotation2295849
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295849/range2295849/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K876011/annotation2295849/range2295849/1
type: Range
persistent identity: range2295849
display id: range2295849
version: 1
direction: direction/reverse
top level: BBa_K876011
start: 987
end: 1143
orientation: inline

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2295865
display id: annotation2295865
version: 1
top level: BBa_K876011
location: https://synbiohub.org/public/igem/BBa_K876011/annotation2295865/range2295865/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K876011/annotation2295865/range2295865/1
type: Range
persistent identity: range2295865
display id: range2295865
version: 1
direction: direction/reverse
top level: BBa_K876011
start: 2311
end: 3084
orientation: inline

BBa_K574006
type: ComponentDefinition
persistent identity: BBa K574006
display id: BBa_K574006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL regulated by pTet and pBad
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Part 2/3 of a Oscillator.
mutable notes: Empty.
mutable provenance: Existing Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K574005
component: BBa_K574004
sequence annotation: BBa_K574005
sequence annotation: BBa_K574004
sequence: https://synbiohub.org/public/igem/BBa_K574006_sequence/1

BBa_K574005
type: Component
persistent identity: component2295507
display id: component2295507
version: 1
top level: BBa_K574006
access: public
definition: BBa_K574005

BBa_K574004
type: Component
persistent identity: component2295487
display id: component2295487
version: 1
top level: BBa_K574006
access: public
definition: https://synbiohub.org/public/igem/BBa_K574004/1

BBa_K574005
type: SequenceAnnotation
persistent identity: annotation2295507
display id: annotation2295507
version: 1
top level: BBa_K574006
location: https://synbiohub.org/public/igem/BBa_K574006/annotation2295507/range2295507/1
component: BBa_K574005

https://synbiohub.org/public/igem/BBa_K574006/annotation2295507/range2295507/1
type: Range
persistent identity: range2295507
display id: range2295507
version: 1
direction: direction/reverse
top level: BBa_K574006
start: 806
end: 2517
orientation: inline

BBa_K574004
type: SequenceAnnotation
persistent identity: annotation2295487
display id: annotation2295487
version: 1
top level: BBa_K574006
location: https://synbiohub.org/public/igem/BBa_K574006/annotation2295487/range2295487/1
component: BBa_K574004

https://synbiohub.org/public/igem/BBa_K574006/annotation2295487/range2295487/1
type: Range
persistent identity: range2295487
display id: range2295487
version: 1
direction: direction/reverse
top level: BBa_K574006
start: 1
end: 797
orientation: inline

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E0022
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_E0022
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
access: public
definition: ECFP

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
direction: direction/forward
top level: BBa_E0422
start: 19
end: 780
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
direction: direction/forward
top level: BBa_E0422
start: 877
end: 917
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
direction: direction/forward
top level: BBa_E0422
start: 789
end: 868
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
direction: direction/forward
top level: BBa_E0422
start: 1
end: 12
orientation: inline

BBa_K737004
type: ComponentDefinition
persistent identity: BBa K737004
display id: BBa_K737004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible GVPA Generator
creator: Li Kang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains a constitutive promoter (J23106), a coding region of LacI protein with an LVA degradation tail, a terminator (B0015), a promoter repressed by LacI protein (R0011) a coding region of GvpA and GFP and a terminator (B0015) behind it. This part can be used to induce the strength of promoter (R0011) by IPTG and indicate the content of GvpA by fluorescence of GFP. In short, it can regulate the expression of GvpA. We can use different concentration of IPTG to regulate the strength of promoter (R0011) ahead GvpA Protein and GFP. And We can know the approximate concentration of GvpA by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP. Here is the curve:
mutable notes: We can use different concentration of IPTG to regulate the strength of promoter (R0011) ahead GvpA Protein and GFP. And We can know the approximate concentration of GvpA by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_P0412
component: BBa_R0011
component: BBa_J23106
component: BBa_E0840
component: BBa_K737016
component: BBa_B0034
sequence annotation: BBa_P0412
sequence annotation: BBa_E0840
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_J23106
sequence annotation: BBa_K737016
sequence: https://synbiohub.org/public/igem/BBa_K737004_sequence/1

BBa_P0412
type: Component
persistent identity: component2184820
display id: component2184820
version: 1
top level: BBa_K737004
access: public
definition: https://synbiohub.org/public/igem/BBa_P0412/1

BBa_R0011
type: Component
persistent identity: component2184821
display id: component2184821
version: 1
top level: BBa_K737004
access: public
definition: lacI+pL

BBa_J23106
type: Component
persistent identity: component2184807
display id: component2184807
version: 1
top level: BBa_K737004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_E0840
type: Component
persistent identity: component2184839
display id: component2184839
version: 1
top level: BBa_K737004
access: public
definition: GFP genera

BBa_K737016
type: Component
persistent identity: component2184828
display id: component2184828
version: 1
top level: BBa_K737004
access: public
definition: https://synbiohub.org/public/igem/BBa_K737016/1

BBa_B0034
type: Component
persistent identity: component2184827
display id: component2184827
version: 1
top level: BBa_K737004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_P0412
type: SequenceAnnotation
persistent identity: annotation2184820
display id: annotation2184820
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184820/range2184820/1
component: BBa_P0412

https://synbiohub.org/public/igem/BBa_K737004/annotation2184820/range2184820/1
type: Range
persistent identity: range2184820
display id: range2184820
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 44
end: 1351
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2184839
display id: annotation2184839
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184839/range2184839/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K737004/annotation2184839/range2184839/1
type: Range
persistent identity: range2184839
display id: range2184839
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 1668
end: 2545
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2184827
display id: annotation2184827
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184827/range2184827/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K737004/annotation2184827/range2184827/1
type: Range
persistent identity: range2184827
display id: range2184827
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 1423
end: 1434
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2184821
display id: annotation2184821
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184821/range2184821/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K737004/annotation2184821/range2184821/1
type: Range
persistent identity: range2184821
display id: range2184821
version: 1
direction: direction/forward
top level: BBa_K737004
start: 1360
end: 1413
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2184807
display id: annotation2184807
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184807/range2184807/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K737004/annotation2184807/range2184807/1
type: Range
persistent identity: range2184807
display id: range2184807
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 1
end: 35
orientation: inline

BBa_K737016
type: SequenceAnnotation
persistent identity: annotation2184828
display id: annotation2184828
version: 1
top level: BBa_K737004
location: https://synbiohub.org/public/igem/BBa_K737004/annotation2184828/range2184828/1
component: BBa_K737016

https://synbiohub.org/public/igem/BBa_K737004/annotation2184828/range2184828/1
type: Range
persistent identity: range2184828
display id: range2184828
version: 1
direction: direction/reverse
top level: BBa_K737004
start: 1441
end: 1659
orientation: inline

BBa_K844000
type: ComponentDefinition
persistent identity: BBa K844000
display id: BBa_K844000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
creator: Kathleen Miller
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1104_
m user id: 0
owner id: 9404
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
mutable notes: none
mutable provenance: Constructed through oligonucleotide annealing
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Stop
sequence annotation: 10x-Histidine Tag
sequence: https://synbiohub.org/public/igem/BBa_K844000_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2206609
display id: annotation2206609
version: 1
top level: BBa_K844000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1

https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
type: Range
persistent identity: range2206609
display id: range2206609
version: 1
direction: direction/forward
top level: BBa_K844000
start: 34
end: 36
orientation: inline

10x-Histidine Tag
type: SequenceAnnotation
persistent identity: annotation2206607
display id: annotation2206607
version: 1
top level: BBa_K844000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1

https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
type: Range
persistent identity: range2206607
display id: range2206607
version: 1
direction: direction/forward
top level: BBa_K844000
start: 1
end: 30
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206608
display id: annotation2206608
version: 1
top level: BBa_K844000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1

https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
type: Range
persistent identity: range2206608
display id: range2206608
version: 1
direction: direction/forward
top level: BBa_K844000
start: 31
end: 33
orientation: inline

BBa_K844007
type: ComponentDefinition
persistent identity: BBa K844007
display id: BBa_K844007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x Subunit "F" (Fewest tRNA codon optimized) with Met (ATG) added
creator: Charles Barentine
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1104_
m user id: 0
owner id: 10131
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spider silk subunit optimized to use the fewest number of tRNA codons; contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.
mutable notes: none
mutable provenance: Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Beta-spiral
sequence annotation: Elasticity Domain
sequence annotation: Start
sequence annotation: Beta-helix
sequence annotation: Strength Domain
sequence annotation: Linker Domain
sequence: https://synbiohub.org/public/igem/BBa_K844007_sequence/1

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212472
display id: annotation2212472
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212472/range2212472/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212472/range2212472/1
type: Range
persistent identity: range2212472
display id: range2212472
version: 1
direction: direction/forward
top level: BBa_K844007
start: 72
end: 87
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212475
display id: annotation2212475
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212475/range2212475/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212475/range2212475/1
type: Range
persistent identity: range2212475
display id: range2212475
version: 1
direction: direction/forward
top level: BBa_K844007
start: 98
end: 113
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212478
display id: annotation2212478
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212478/range2212478/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212478/range2212478/1
type: Range
persistent identity: range2212478
display id: range2212478
version: 1
direction: direction/forward
top level: BBa_K844007
start: 156
end: 171
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212467
display id: annotation2212467
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212467/range2212467/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212467/range2212467/1
type: Range
persistent identity: range2212467
display id: range2212467
version: 1
direction: direction/forward
top level: BBa_K844007
start: 4
end: 87
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212476
display id: annotation2212476
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212476/range2212476/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212476/range2212476/1
type: Range
persistent identity: range2212476
display id: range2212476
version: 1
direction: direction/forward
top level: BBa_K844007
start: 117
end: 132
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212473
display id: annotation2212473
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212473/range2212473/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212473/range2212473/1
type: Range
persistent identity: range2212473
display id: range2212473
version: 1
direction: direction/forward
top level: BBa_K844007
start: 88
end: 171
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2212466
display id: annotation2212466
version: 1
top level: BBa_K844007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212466/range2212466/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212466/range2212466/1
type: Range
persistent identity: range2212466
display id: range2212466
version: 1
direction: direction/forward
top level: BBa_K844007
start: 1
end: 3
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212477
display id: annotation2212477
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212477/range2212477/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212477/range2212477/1
type: Range
persistent identity: range2212477
display id: range2212477
version: 1
direction: direction/forward
top level: BBa_K844007
start: 133
end: 147
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212474
display id: annotation2212474
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212474/range2212474/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212474/range2212474/1
type: Range
persistent identity: range2212474
display id: range2212474
version: 1
direction: direction/forward
top level: BBa_K844007
start: 88
end: 97
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212468
display id: annotation2212468
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212468/range2212468/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212468/range2212468/1
type: Range
persistent identity: range2212468
display id: range2212468
version: 1
direction: direction/forward
top level: BBa_K844007
start: 4
end: 12
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212470
display id: annotation2212470
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212470/range2212470/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212470/range2212470/1
type: Range
persistent identity: range2212470
display id: range2212470
version: 1
direction: direction/forward
top level: BBa_K844007
start: 34
end: 48
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212480
display id: annotation2212480
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212480/range2212480/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212480/range2212480/1
type: Range
persistent identity: range2212480
display id: range2212480
version: 1
direction: direction/forward
top level: BBa_K844007
start: 189
end: 207
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212471
display id: annotation2212471
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212471/range2212471/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212471/range2212471/1
type: Range
persistent identity: range2212471
display id: range2212471
version: 1
direction: direction/forward
top level: BBa_K844007
start: 49
end: 63
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212479
display id: annotation2212479
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212479/range2212479/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212479/range2212479/1
type: Range
persistent identity: range2212479
display id: range2212479
version: 1
direction: direction/forward
top level: BBa_K844007
start: 172
end: 188
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212469
display id: annotation2212469
version: 1
top level: BBa_K844007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844007/annotation2212469/range2212469/1

https://synbiohub.org/public/igem/BBa_K844007/annotation2212469/range2212469/1
type: Range
persistent identity: range2212469
display id: range2212469
version: 1
direction: direction/forward
top level: BBa_K844007
start: 13
end: 27
orientation: inline

BBa_K747032
type: ComponentDefinition
persistent identity: BBa K747032
display id: BBa_K747032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 3
sequence: https://synbiohub.org/public/igem/BBa_K747032_sequence/1

AA 3
type: SequenceAnnotation
persistent identity: annotation2196207
display id: annotation2196207
version: 1
top level: BBa_K747032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747032/annotation2196207/range2196207/1

https://synbiohub.org/public/igem/BBa_K747032/annotation2196207/range2196207/1
type: Range
persistent identity: range2196207
display id: range2196207
version: 1
direction: direction/forward
top level: BBa_K747032
start: 14
end: 211
orientation: inline

BBa_K747040
type: ComponentDefinition
persistent identity: BBa K747040
display id: BBa_K747040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 3
sequence: https://synbiohub.org/public/igem/BBa_K747040_sequence/1

GA 3
type: SequenceAnnotation
persistent identity: annotation2196250
display id: annotation2196250
version: 1
top level: BBa_K747040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747040/annotation2196250/range2196250/1

https://synbiohub.org/public/igem/BBa_K747040/annotation2196250/range2196250/1
type: Range
persistent identity: range2196250
display id: range2196250
version: 1
direction: direction/forward
top level: BBa_K747040
start: 14
end: 211
orientation: inline

BBa_K747094
type: ComponentDefinition
persistent identity: BBa K747094
display id: BBa_K747094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 6
sequence: https://synbiohub.org/public/igem/BBa_K747094_sequence/1

TC 6
type: SequenceAnnotation
persistent identity: annotation2197072
display id: annotation2197072
version: 1
top level: BBa_K747094
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747094/annotation2197072/range2197072/1

https://synbiohub.org/public/igem/BBa_K747094/annotation2197072/range2197072/1
type: Range
persistent identity: range2197072
display id: range2197072
version: 1
direction: direction/forward
top level: BBa_K747094
start: 14
end: 211
orientation: inline

BBa_K747026
type: ComponentDefinition
persistent identity: BBa K747026
display id: BBa_K747026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 2
sequence: https://synbiohub.org/public/igem/BBa_K747026_sequence/1

GG 2
type: SequenceAnnotation
persistent identity: annotation2196132
display id: annotation2196132
version: 1
top level: BBa_K747026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747026/annotation2196132/range2196132/1

https://synbiohub.org/public/igem/BBa_K747026/annotation2196132/range2196132/1
type: Range
persistent identity: range2196132
display id: range2196132
version: 1
direction: direction/forward
top level: BBa_K747026
start: 14
end: 211
orientation: inline

BBa_K747046
type: ComponentDefinition
persistent identity: BBa K747046
display id: BBa_K747046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 3
sequence: https://synbiohub.org/public/igem/BBa_K747046_sequence/1

TG 3
type: SequenceAnnotation
persistent identity: annotation2196268
display id: annotation2196268
version: 1
top level: BBa_K747046
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747046/annotation2196268/range2196268/1

https://synbiohub.org/public/igem/BBa_K747046/annotation2196268/range2196268/1
type: Range
persistent identity: range2196268
display id: range2196268
version: 1
direction: direction/forward
top level: BBa_K747046
start: 14
end: 211
orientation: inline

BBa_K747092
type: ComponentDefinition
persistent identity: BBa K747092
display id: BBa_K747092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 6
sequence: https://synbiohub.org/public/igem/BBa_K747092_sequence/1

TA 6
type: SequenceAnnotation
persistent identity: annotation2197070
display id: annotation2197070
version: 1
top level: BBa_K747092
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747092/annotation2197070/range2197070/1

https://synbiohub.org/public/igem/BBa_K747092/annotation2197070/range2197070/1
type: Range
persistent identity: range2197070
display id: range2197070
version: 1
direction: direction/forward
top level: BBa_K747092
start: 14
end: 211
orientation: inline

BBa_K747031
type: ComponentDefinition
persistent identity: BBa K747031
display id: BBa_K747031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 2
sequence: https://synbiohub.org/public/igem/BBa_K747031_sequence/1

TT 2
type: SequenceAnnotation
persistent identity: annotation2196206
display id: annotation2196206
version: 1
top level: BBa_K747031
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747031/annotation2196206/range2196206/1

https://synbiohub.org/public/igem/BBa_K747031/annotation2196206/range2196206/1
type: Range
persistent identity: range2196206
display id: range2196206
version: 1
direction: direction/forward
top level: BBa_K747031
start: 14
end: 211
orientation: inline

BBa_K775009
type: ComponentDefinition
persistent identity: BBa K775009
display id: BBa_K775009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Niacin Chimeric GPCR with Fus1-EGFP reporter (2)
creator: Sietske Grijseels
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K775000
component: BBa_K775004
sequence annotation: BBa_K775000
sequence annotation: BBa_K775004
sequence: https://synbiohub.org/public/igem/BBa_K775009_sequence/1

BBa_K775000
type: Component
persistent identity: component2195587
display id: component2195587
version: 1
top level: BBa_K775009
access: public
definition: BBa_K775000

BBa_K775004
type: Component
persistent identity: component2195575
display id: component2195575
version: 1
top level: BBa_K775009
access: public
definition: https://synbiohub.org/public/igem/BBa_K775004/1

BBa_K775000
type: SequenceAnnotation
persistent identity: annotation2195587
display id: annotation2195587
version: 1
top level: BBa_K775009
location: https://synbiohub.org/public/igem/BBa_K775009/annotation2195587/range2195587/1
component: BBa_K775000

https://synbiohub.org/public/igem/BBa_K775009/annotation2195587/range2195587/1
type: Range
persistent identity: range2195587
display id: range2195587
version: 1
direction: direction/reverse
top level: BBa_K775009
start: 1175
end: 2588
orientation: inline

BBa_K775004
type: SequenceAnnotation
persistent identity: annotation2195575
display id: annotation2195575
version: 1
top level: BBa_K775009
location: https://synbiohub.org/public/igem/BBa_K775009/annotation2195575/range2195575/1
component: BBa_K775004

https://synbiohub.org/public/igem/BBa_K775009/annotation2195575/range2195575/1
type: Range
persistent identity: range2195575
display id: range2195575
version: 1
direction: direction/reverse
top level: BBa_K775009
start: 1
end: 1166
orientation: inline

BBa_K784014
type: ComponentDefinition
persistent identity: BBa K784014
display id: BBa_K784014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux+Spacer2+RBS+mCherry
creator: Ilya Vainberg Slutskin
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: false
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequence is the product of restriction cloning of the spacer2+RBS+mCherry downstream to the pLux promoter. In the presence of LuxR transcription can be induced by 3OC6HSL.
mutable notes: No design considerations.
mutable provenance: Cloning of the spacer2+RBS+mCherry downstream to the pLux promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K784009
sequence annotation: BBa_R0062
sequence annotation: BBa_K784009
sequence: https://synbiohub.org/public/igem/BBa_K784014_sequence/1

BBa_R0062
type: Component
persistent identity: component2183696
display id: component2183696
version: 1
top level: BBa_K784014
access: public
definition: lux pR

BBa_K784009
type: Component
persistent identity: component2183708
display id: component2183708
version: 1
top level: BBa_K784014
access: public
definition: https://synbiohub.org/public/igem/BBa_K784009/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2183696
display id: annotation2183696
version: 1
top level: BBa_K784014
location: https://synbiohub.org/public/igem/BBa_K784014/annotation2183696/range2183696/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K784014/annotation2183696/range2183696/1
type: Range
persistent identity: range2183696
display id: range2183696
version: 1
direction: direction/forward
top level: BBa_K784014
start: 1
end: 55
orientation: inline

BBa_K784009
type: SequenceAnnotation
persistent identity: annotation2183708
display id: annotation2183708
version: 1
top level: BBa_K784014
location: https://synbiohub.org/public/igem/BBa_K784014/annotation2183708/range2183708/1
component: BBa_K784009

https://synbiohub.org/public/igem/BBa_K784014/annotation2183708/range2183708/1
type: Range
persistent identity: range2183708
display id: range2183708
version: 1
direction: direction/reverse
top level: BBa_K784014
start: 64
end: 828
orientation: inline

BBa_J01142
type: ComponentDefinition
persistent identity: BBa J01142
display id: BBa_J01142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [pLacI pL][key3]
creator: Golden Bear (Berkeley iGEM 2005)
created: 2006-04-13T11:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_1_
m user id: 0
owner id: 612
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_R0011
component: BBa_J01086
sequence annotation: BBa_R0011
sequence annotation: BBa_J01086
sequence: https://synbiohub.org/public/igem/BBa_J01142_sequence/1

BBa_R0011
type: Component
persistent identity: component1843668
display id: component1843668
version: 1
top level: BBa_J01142
access: public
definition: lacI+pL

BBa_J01086
type: Component
persistent identity: component1843674
display id: component1843674
version: 1
top level: BBa_J01142
access: public
definition: https://synbiohub.org/public/igem/BBa_J01086/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1843668
display id: annotation1843668
version: 1
top level: BBa_J01142
location: https://synbiohub.org/public/igem/BBa_J01142/annotation1843668/range1843668/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J01142/annotation1843668/range1843668/1
type: Range
persistent identity: range1843668
display id: range1843668
version: 1
direction: direction/forward
top level: BBa_J01142
start: 1
end: 54
orientation: inline

BBa_J01086
type: SequenceAnnotation
persistent identity: annotation1843674
display id: annotation1843674
version: 1
top level: BBa_J01142
location: https://synbiohub.org/public/igem/BBa_J01142/annotation1843674/range1843674/1
component: BBa_J01086

https://synbiohub.org/public/igem/BBa_J01142/annotation1843674/range1843674/1
type: Range
persistent identity: range1843674
display id: range1843674
version: 1
direction: direction/reverse
top level: BBa_J01142
start: 64
end: 157
orientation: inline

BBa_K823016
type: ComponentDefinition
persistent identity: BBa K823016
display id: BBa_K823016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ full-length (RBS+ TERM-)
creator: Jara Radeck
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Full-length lacZ with an RBS ahead. This is a copy of the Part:BBa_I732017. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1A2.
mutable notes: We changed the backbone vector of the part from pSB1A2 to pSB1C3
mutable provenance: Part:BBa_I732017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I732017
sequence annotation: BBa_I732017
sequence: https://synbiohub.org/public/igem/BBa_K823016_sequence/1

BBa_I732017
type: Component
persistent identity: component2182650
display id: component2182650
version: 1
top level: BBa_K823016
access: public
definition: https://synbiohub.org/public/igem/BBa_I732017/1

BBa_I732017
type: SequenceAnnotation
persistent identity: annotation2182650
display id: annotation2182650
version: 1
top level: BBa_K823016
location: https://synbiohub.org/public/igem/BBa_K823016/annotation2182650/range2182650/1
component: BBa_I732017

https://synbiohub.org/public/igem/BBa_K823016/annotation2182650/range2182650/1
type: Range
persistent identity: range2182650
display id: range2182650
version: 1
direction: direction/reverse
top level: BBa_K823016
start: 1
end: 3093
orientation: inline

BBa_K747014
type: ComponentDefinition
persistent identity: BBa K747014
display id: BBa_K747014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 1
sequence: https://synbiohub.org/public/igem/BBa_K747014_sequence/1

TG 1
type: SequenceAnnotation
persistent identity: annotation2195929
display id: annotation2195929
version: 1
top level: BBa_K747014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747014/annotation2195929/range2195929/1

https://synbiohub.org/public/igem/BBa_K747014/annotation2195929/range2195929/1
type: Range
persistent identity: range2195929
display id: range2195929
version: 1
direction: direction/forward
top level: BBa_K747014
start: 12
end: 209
orientation: inline

BBa_B0013
type: ComponentDefinition
persistent identity: BBa B0013
display id: BBa_B0013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphage T7 (+/-)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0013
sequence annotation: T7 TE
sequence annotation: ''
sequence annotation: C
sequence annotation: stop
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_B0013_sequence/1

BBa_B0013
type: SequenceAnnotation
persistent identity: annotation7021
display id: annotation7021
version: 1
top level: BBa_B0013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1

https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1
type: Range
persistent identity: range7021
display id: range7021
version: 1
direction: direction/forward
top level: BBa_B0013
start: 1
end: 47
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1691
display id: annotation1691
version: 1
top level: BBa_B0013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1
type: Range
persistent identity: range1691
display id: range1691
version: 1
direction: direction/forward
top level: BBa_B0013
start: 14
end: 33
orientation: inline

''
type: SequenceAnnotation
persistent identity: annotation1697
display id: annotation1697
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1
type: Range
persistent identity: range1697
display id: range1697
version: 1
direction: direction/forward
top level: BBa_B0013
start: 1
end: 6
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1695
display id: annotation1695
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1
type: Range
persistent identity: range1695
display id: range1695
version: 1
direction: direction/forward
top level: BBa_B0013
start: 37
end: 37
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1692
display id: annotation1692
version: 1
top level: BBa_B0013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1
type: Range
persistent identity: range1692
display id: range1692
version: 1
direction: direction/forward
top level: BBa_B0013
start: 40
end: 40
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1698
display id: annotation1698
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1
type: Range
persistent identity: range1698
display id: range1698
version: 1
direction: direction/forward
top level: BBa_B0013
start: 34
end: 47
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1696
display id: annotation1696
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1
type: Range
persistent identity: range1696
display id: range1696
version: 1
direction: direction/forward
top level: BBa_B0013
start: 10
end: 10
orientation: inline

BBa_K880005
type: ComponentDefinition
persistent identity: BBa K880005
display id: BBa_K880005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong promoter, strong RBS combination for high expression levels of proteins
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
mutable notes: Enter design considerations.
mutable provenance: It is a combination of strong promoter (J23100) and RBS (B0034).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K880005_sequence/1

BBa_B0034
type: Component
persistent identity: component2204230
display id: component2204230
version: 1
top level: BBa_K880005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: Component
persistent identity: component2204228
display id: component2204228
version: 1
top level: BBa_K880005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2204228
display id: annotation2204228
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
type: Range
persistent identity: range2204228
display id: range2204228
version: 1
direction: direction/reverse
top level: BBa_K880005
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2204230
display id: annotation2204230
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
type: Range
persistent identity: range2204230
display id: range2204230
version: 1
direction: direction/reverse
top level: BBa_K880005
start: 44
end: 55
orientation: inline

BBa_K747047
type: ComponentDefinition
persistent identity: BBa K747047
display id: BBa_K747047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 3
sequence: https://synbiohub.org/public/igem/BBa_K747047_sequence/1

TT 3
type: SequenceAnnotation
persistent identity: annotation2196270
display id: annotation2196270
version: 1
top level: BBa_K747047
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747047/annotation2196270/range2196270/1

https://synbiohub.org/public/igem/BBa_K747047/annotation2196270/range2196270/1
type: Range
persistent identity: range2196270
display id: range2196270
version: 1
direction: direction/forward
top level: BBa_K747047
start: 14
end: 211
orientation: inline

BBa_K567018
type: ComponentDefinition
persistent identity: BBa K567018
display id: BBa_K567018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-GFP-TAG-RFP
creator: Xiaopan Ma and Xiwen Zhao
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP and RFP linked with a flexible chain and a stop codon TAG is inserted in the flexible chain. This biobrick is under the control of T7 promoter and lac operator. This part is used to testify the function of PT7-TDRS (BBa_K567011) and tRNA(Asp)-TAG (BBa_K567013).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP-TAG-RFP
sequence annotation: GFP
sequence annotation: T7 TE
sequence annotation: mRFP
sequence annotation: TAG
sequence annotation: lac operator
sequence annotation: PT7
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K567018_sequence/1

GFP-TAG-RFP
type: SequenceAnnotation
persistent identity: annotation2154491
display id: annotation2154491
version: 1
top level: BBa_K567018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154491/range2154491/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154491/range2154491/1
type: Range
persistent identity: range2154491
display id: range2154491
version: 1
direction: direction/forward
top level: BBa_K567018
start: 89
end: 1621
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2154350
display id: annotation2154350
version: 1
top level: BBa_K567018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154350/range2154350/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154350/range2154350/1
type: Range
persistent identity: range2154350
display id: range2154350
version: 1
direction: direction/forward
top level: BBa_K567018
start: 197
end: 910
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2154492
display id: annotation2154492
version: 1
top level: BBa_K567018
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154492/range2154492/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154492/range2154492/1
type: Range
persistent identity: range2154492
display id: range2154492
version: 1
direction: direction/forward
top level: BBa_K567018
start: 1712
end: 1759
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2154469
display id: annotation2154469
version: 1
top level: BBa_K567018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154469/range2154469/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154469/range2154469/1
type: Range
persistent identity: range2154469
display id: range2154469
version: 1
direction: direction/forward
top level: BBa_K567018
start: 944
end: 1621
orientation: inline

TAG
type: SequenceAnnotation
persistent identity: annotation2154444
display id: annotation2154444
version: 1
top level: BBa_K567018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154444/range2154444/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154444/range2154444/1
type: Range
persistent identity: range2154444
display id: range2154444
version: 1
direction: direction/forward
top level: BBa_K567018
start: 926
end: 928
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2154347
display id: annotation2154347
version: 1
top level: BBa_K567018
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154347/range2154347/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154347/range2154347/1
type: Range
persistent identity: range2154347
display id: range2154347
version: 1
direction: direction/forward
top level: BBa_K567018
start: 21
end: 44
orientation: inline

PT7
type: SequenceAnnotation
persistent identity: annotation2154346
display id: annotation2154346
version: 1
top level: BBa_K567018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154346/range2154346/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154346/range2154346/1
type: Range
persistent identity: range2154346
display id: range2154346
version: 1
direction: direction/forward
top level: BBa_K567018
start: 2
end: 18
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154349
display id: annotation2154349
version: 1
top level: BBa_K567018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567018/annotation2154349/range2154349/1

https://synbiohub.org/public/igem/BBa_K567018/annotation2154349/range2154349/1
type: Range
persistent identity: range2154349
display id: range2154349
version: 1
direction: direction/forward
top level: BBa_K567018
start: 75
end: 81
orientation: inline

BBa_K747093
type: ComponentDefinition
persistent identity: BBa K747093
display id: BBa_K747093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 6
sequence: https://synbiohub.org/public/igem/BBa_K747093_sequence/1

TC 6
type: SequenceAnnotation
persistent identity: annotation2197071
display id: annotation2197071
version: 1
top level: BBa_K747093
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747093/annotation2197071/range2197071/1

https://synbiohub.org/public/igem/BBa_K747093/annotation2197071/range2197071/1
type: Range
persistent identity: range2197071
display id: range2197071
version: 1
direction: direction/forward
top level: BBa_K747093
start: 14
end: 211
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI repressor from E. coli (+LVA)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
direction: direction/forward
top level: lacI
start: 1129
end: 1153
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
direction: direction/forward
top level: lacI
start: 1090
end: 1128
orientation: inline

BBa_K747002
type: ComponentDefinition
persistent identity: BBa K747002
display id: BBa_K747002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 1
sequence: https://synbiohub.org/public/igem/BBa_K747002_sequence/1

AG 1
type: SequenceAnnotation
persistent identity: annotation2195763
display id: annotation2195763
version: 1
top level: BBa_K747002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747002/annotation2195763/range2195763/1

https://synbiohub.org/public/igem/BBa_K747002/annotation2195763/range2195763/1
type: Range
persistent identity: range2195763
display id: range2195763
version: 1
direction: direction/forward
top level: BBa_K747002
start: 12
end: 209
orientation: inline

BBa_S0107
type: ComponentDefinition
persistent identity: BBa S0107
display id: BBa_S0107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0033.C0040
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0040
component: BBa_B0033
sequence annotation: BBa_C0040
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_S0107_sequence/1

BBa_C0040
type: Component
persistent identity: component939826
display id: component939826
version: 1
top level: BBa_S0107
access: public
definition: tetR

BBa_B0033
type: Component
persistent identity: component939815
display id: component939815
version: 1
top level: BBa_S0107
access: public
definition: BBa_B0033

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation939826
display id: annotation939826
version: 1
top level: BBa_S0107
location: https://synbiohub.org/public/igem/BBa_S0107/annotation939826/range939826/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_S0107/annotation939826/range939826/1
type: Range
persistent identity: range939826
display id: range939826
version: 1
direction: direction/forward
top level: BBa_S0107
start: 18
end: 677
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939815
display id: annotation939815
version: 1
top level: BBa_S0107
location: https://synbiohub.org/public/igem/BBa_S0107/annotation939815/range939815/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_S0107/annotation939815/range939815/1
type: Range
persistent identity: range939815
display id: range939815
version: 1
direction: direction/forward
top level: BBa_S0107
start: 1
end: 11
orientation: inline

BBa_K916000
type: ComponentDefinition
persistent identity: BBa K916000
display id: BBa_K916000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TraR Coding Sequence
creator: Joseph Elsherbini
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1181_
m user id: 0
owner id: 4259
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TraR Coding Sequence from Agrobacterium tumefaciens
mutable notes: -
mutable provenance: plasmid containing TraR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: cds
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K916000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2202116
display id: annotation2202116
version: 1
top level: BBa_K916000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K916000/annotation2202116/range2202116/1

https://synbiohub.org/public/igem/BBa_K916000/annotation2202116/range2202116/1
type: Range
persistent identity: range2202116
display id: range2202116
version: 1
direction: direction/forward
top level: BBa_K916000
start: 703
end: 705
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2202115
display id: annotation2202115
version: 1
top level: BBa_K916000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K916000/annotation2202115/range2202115/1

https://synbiohub.org/public/igem/BBa_K916000/annotation2202115/range2202115/1
type: Range
persistent identity: range2202115
display id: range2202115
version: 1
direction: direction/forward
top level: BBa_K916000
start: 1
end: 705
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2202117
display id: annotation2202117
version: 1
top level: BBa_K916000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K916000/annotation2202117/range2202117/1

https://synbiohub.org/public/igem/BBa_K916000/annotation2202117/range2202117/1
type: Range
persistent identity: range2202117
display id: range2202117
version: 1
direction: direction/forward
top level: BBa_K916000
start: 1
end: 3
orientation: inline

BBa_I14044
type: ComponentDefinition
persistent identity: BBa I14044
display id: BBa_I14044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weak RBS . LacI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: BBa_B0031 BBa_C0012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0012
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_I14044_sequence/1

BBa_C0012
type: Component
persistent identity: component1041746
display id: component1041746
version: 1
top level: BBa_I14044
access: public
definition: lacI

BBa_B0031
type: Component
persistent identity: component1041736
display id: component1041736
version: 1
top level: BBa_I14044
access: public
definition: BBa_B0031

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1041736
display id: annotation1041736
version: 1
top level: BBa_I14044
location: https://synbiohub.org/public/igem/BBa_I14044/annotation1041736/range1041736/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I14044/annotation1041736/range1041736/1
type: Range
persistent identity: range1041736
display id: range1041736
version: 1
direction: direction/forward
top level: BBa_I14044
start: 1
end: 14
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1041746
display id: annotation1041746
version: 1
top level: BBa_I14044
location: https://synbiohub.org/public/igem/BBa_I14044/annotation1041746/range1041746/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I14044/annotation1041746/range1041746/1
type: Range
persistent identity: range1041746
display id: range1041746
version: 1
direction: direction/forward
top level: BBa_I14044
start: 21
end: 1148
orientation: inline

BBa_K747052
type: ComponentDefinition
persistent identity: BBa K747052
display id: BBa_K747052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CA4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 4
sequence: https://synbiohub.org/public/igem/BBa_K747052_sequence/1

AC 4
type: SequenceAnnotation
persistent identity: annotation2196283
display id: annotation2196283
version: 1
top level: BBa_K747052
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747052/annotation2196283/range2196283/1

https://synbiohub.org/public/igem/BBa_K747052/annotation2196283/range2196283/1
type: Range
persistent identity: range2196283
display id: range2196283
version: 1
direction: direction/forward
top level: BBa_K747052
start: 14
end: 211
orientation: inline

BBa_J202014
type: ComponentDefinition
persistent identity: BBa J202014
display id: BBa_J202014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#15
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -59 relative to the araC transcriptional start site was changed from C to T.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mutation
sequence annotation: araI1
sequence annotation: Transcription Start
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202014_sequence/1

Mutation
type: SequenceAnnotation
persistent identity: annotation2178473
display id: annotation2178473
version: 1
top level: BBa_J202014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202014/annotation2178473/range2178473/1

https://synbiohub.org/public/igem/BBa_J202014/annotation2178473/range2178473/1
type: Range
persistent identity: range2178473
display id: range2178473
version: 1
direction: direction/forward
top level: BBa_J202014
start: 53
end: 53
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178475
display id: annotation2178475
version: 1
top level: BBa_J202014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202014/annotation2178475/range2178475/1

https://synbiohub.org/public/igem/BBa_J202014/annotation2178475/range2178475/1
type: Range
persistent identity: range2178475
display id: range2178475
version: 1
direction: direction/forward
top level: BBa_J202014
start: 40
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178474
display id: annotation2178474
version: 1
top level: BBa_J202014
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202014/annotation2178474/range2178474/1

https://synbiohub.org/public/igem/BBa_J202014/annotation2178474/range2178474/1
type: Range
persistent identity: range2178474
display id: range2178474
version: 1
direction: direction/forward
top level: BBa_J202014
start: 112
end: 112
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178476
display id: annotation2178476
version: 1
top level: BBa_J202014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202014/annotation2178476/range2178476/1

https://synbiohub.org/public/igem/BBa_J202014/annotation2178476/range2178476/1
type: Range
persistent identity: range2178476
display id: range2178476
version: 1
direction: direction/forward
top level: BBa_J202014
start: 61
end: 77
orientation: inline

BBa_K953002
type: ComponentDefinition
persistent identity: BBa K953002
display id: BBa_K953002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)
creator: Daniel Winter
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1221_
m user id: 0
owner id: 14275
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells.
mutable notes: Optimised for expression in E. coli
mutable provenance: Agrobacterium tumefaciens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Start Codon
sequence annotation: Stop
sequence annotation: Bacteriophytochrome
sequence annotation: BBa_K953002
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K953002_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2196369
display id: annotation2196369
version: 1
top level: BBa_K953002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196369/range2196369/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196369/range2196369/1
type: Range
persistent identity: range2196369
display id: range2196369
version: 1
direction: direction/forward
top level: BBa_K953002
start: 23
end: 25
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2196371
display id: annotation2196371
version: 1
top level: BBa_K953002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196371/range2196371/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196371/range2196371/1
type: Range
persistent identity: range2196371
display id: range2196371
version: 1
direction: direction/forward
top level: BBa_K953002
start: 2231
end: 2236
orientation: inline

Bacteriophytochrome
type: SequenceAnnotation
persistent identity: annotation2196370
display id: annotation2196370
version: 1
top level: BBa_K953002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196370/range2196370/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196370/range2196370/1
type: Range
persistent identity: range2196370
display id: range2196370
version: 1
direction: direction/forward
top level: BBa_K953002
start: 26
end: 2230
orientation: inline

BBa_K953002
type: SequenceAnnotation
persistent identity: annotation2196372
display id: annotation2196372
version: 1
top level: BBa_K953002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196372/range2196372/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196372/range2196372/1
type: Range
persistent identity: range2196372
display id: range2196372
version: 1
direction: direction/reverse
top level: BBa_K953002
start: 1
end: 2236
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2196368
display id: annotation2196368
version: 1
top level: BBa_K953002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K953002/annotation2196368/range2196368/1

https://synbiohub.org/public/igem/BBa_K953002/annotation2196368/range2196368/1
type: Range
persistent identity: range2196368
display id: range2196368
version: 1
direction: direction/forward
top level: BBa_K953002
start: 1
end: 22
orientation: inline

BBa_K775003
type: ComponentDefinition
persistent identity: BBa K775003
display id: BBa_K775003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator
creator: Sietske Grijseels
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: -
mutable provenance: N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: C. elegans 7-transmembrane receptor ODR-10 U49449.1 C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K775003_sequence/1

BBa_K909006
type: ComponentDefinition
persistent identity: BBa K909006
display id: BBa_K909006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full-length lacZ with RBS and Terminator
creator: Isaak Mueller
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14050
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I732017
component: BBa_B0017
sequence annotation: BBa_B0017
sequence annotation: BBa_I732017
sequence: https://synbiohub.org/public/igem/BBa_K909006_sequence/1

BBa_I732017
type: Component
persistent identity: component2195194
display id: component2195194
version: 1
top level: BBa_K909006
access: public
definition: https://synbiohub.org/public/igem/BBa_I732017/1

BBa_B0017
type: Component
persistent identity: component2195199
display id: component2195199
version: 1
top level: BBa_K909006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2195199
display id: annotation2195199
version: 1
top level: BBa_K909006
location: https://synbiohub.org/public/igem/BBa_K909006/annotation2195199/range2195199/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K909006/annotation2195199/range2195199/1
type: Range
persistent identity: range2195199
display id: range2195199
version: 1
direction: direction/reverse
top level: BBa_K909006
start: 3102
end: 3269
orientation: inline

BBa_I732017
type: SequenceAnnotation
persistent identity: annotation2195194
display id: annotation2195194
version: 1
top level: BBa_K909006
location: https://synbiohub.org/public/igem/BBa_K909006/annotation2195194/range2195194/1
component: BBa_I732017

https://synbiohub.org/public/igem/BBa_K909006/annotation2195194/range2195194/1
type: Range
persistent identity: range2195194
display id: range2195194
version: 1
direction: direction/reverse
top level: BBa_K909006
start: 1
end: 3093
orientation: inline

BBa_K747008
type: ComponentDefinition
persistent identity: BBa K747008
display id: BBa_K747008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 1
sequence: https://synbiohub.org/public/igem/BBa_K747008_sequence/1

GA 1
type: SequenceAnnotation
persistent identity: annotation2195824
display id: annotation2195824
version: 1
top level: BBa_K747008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747008/annotation2195824/range2195824/1

https://synbiohub.org/public/igem/BBa_K747008/annotation2195824/range2195824/1
type: Range
persistent identity: range2195824
display id: range2195824
version: 1
direction: direction/forward
top level: BBa_K747008
start: 12
end: 209
orientation: inline

BBa_K747069
type: ComponentDefinition
persistent identity: BBa K747069
display id: BBa_K747069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 5
sequence: https://synbiohub.org/public/igem/BBa_K747069_sequence/1

CC 5
type: SequenceAnnotation
persistent identity: annotation2197032
display id: annotation2197032
version: 1
top level: BBa_K747069
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747069/annotation2197032/range2197032/1

https://synbiohub.org/public/igem/BBa_K747069/annotation2197032/range2197032/1
type: Range
persistent identity: range2197032
display id: range2197032
version: 1
direction: direction/forward
top level: BBa_K747069
start: 14
end: 211
orientation: inline

BBa_K747037
type: ComponentDefinition
persistent identity: BBa K747037
display id: BBa_K747037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 3
sequence: https://synbiohub.org/public/igem/BBa_K747037_sequence/1

CC 3
type: SequenceAnnotation
persistent identity: annotation2196216
display id: annotation2196216
version: 1
top level: BBa_K747037
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747037/annotation2196216/range2196216/1

https://synbiohub.org/public/igem/BBa_K747037/annotation2196216/range2196216/1
type: Range
persistent identity: range2196216
display id: range2196216
version: 1
direction: direction/forward
top level: BBa_K747037
start: 14
end: 211
orientation: inline

BBa_I6032
type: ComponentDefinition
persistent identity: BBa I6032
display id: BBa_I6032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0040.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0040
component: BBa_B0012
component: BBa_E0030
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I6032_sequence/1

BBa_R0040
type: Component
persistent identity: component948347
display id: component948347
version: 1
top level: BBa_I6032
access: public
definition: p(tetR)

BBa_B0012
type: Component
persistent identity: component948372
display id: component948372
version: 1
top level: BBa_I6032
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component948357
display id: component948357
version: 1
top level: BBa_I6032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component948355
display id: component948355
version: 1
top level: BBa_I6032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component948362
display id: component948362
version: 1
top level: BBa_I6032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948357
display id: annotation948357
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948357/range948357/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6032/annotation948357/range948357/1
type: Range
persistent identity: range948357
display id: range948357
version: 1
direction: direction/forward
top level: BBa_I6032
start: 81
end: 803
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948355
display id: annotation948355
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948355/range948355/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6032/annotation948355/range948355/1
type: Range
persistent identity: range948355
display id: range948355
version: 1
direction: direction/forward
top level: BBa_I6032
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948362
display id: annotation948362
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948362/range948362/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6032/annotation948362/range948362/1
type: Range
persistent identity: range948362
display id: range948362
version: 1
direction: direction/forward
top level: BBa_I6032
start: 812
end: 891
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948372
display id: annotation948372
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948372/range948372/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6032/annotation948372/range948372/1
type: Range
persistent identity: range948372
display id: range948372
version: 1
direction: direction/forward
top level: BBa_I6032
start: 900
end: 940
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation948347
display id: annotation948347
version: 1
top level: BBa_I6032
location: https://synbiohub.org/public/igem/BBa_I6032/annotation948347/range948347/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I6032/annotation948347/range948347/1
type: Range
persistent identity: range948347
display id: range948347
version: 1
direction: direction/forward
top level: BBa_I6032
start: 1
end: 54
orientation: inline

BBa_I13457
type: ComponentDefinition
persistent identity: BBa I13457
display id: BBa_I13457
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Improved insertion site with two RNAse E cut sites
creator: jkm
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Shortened RNAse E site, insertion site, duplicate RNAse E site, and a trailing 5' hairpin (HP16 from Smolke et al., 2002).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RNAse E cut site (between the bases)
sequence annotation: Hairpin
sequence annotation: Insertion site
sequence annotation: RNAse E site
sequence: https://synbiohub.org/public/igem/BBa_I13457_sequence/1

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588511
display id: annotation1588511
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588511/range1588511/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588511/range1588511/1
type: Range
persistent identity: range1588511
display id: range1588511
version: 1
direction: direction/forward
top level: BBa_I13457
start: 9
end: 10
orientation: inline

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588514
display id: annotation1588514
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588514/range1588514/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588514/range1588514/1
type: Range
persistent identity: range1588514
display id: range1588514
version: 1
direction: direction/forward
top level: BBa_I13457
start: 64
end: 65
orientation: inline

Hairpin
type: SequenceAnnotation
persistent identity: annotation1588515
display id: annotation1588515
version: 1
top level: BBa_I13457
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588515/range1588515/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588515/range1588515/1
type: Range
persistent identity: range1588515
display id: range1588515
version: 1
direction: direction/forward
top level: BBa_I13457
start: 77
end: 111
orientation: inline

Insertion site
type: SequenceAnnotation
persistent identity: annotation1588512
display id: annotation1588512
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588512/range1588512/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588512/range1588512/1
type: Range
persistent identity: range1588512
display id: range1588512
version: 1
direction: direction/forward
top level: BBa_I13457
start: 22
end: 55
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588513
display id: annotation1588513
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588513/range1588513/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588513/range1588513/1
type: Range
persistent identity: range1588513
display id: range1588513
version: 1
direction: direction/forward
top level: BBa_I13457
start: 56
end: 76
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588510
display id: annotation1588510
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588510/range1588510/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588510/range1588510/1
type: Range
persistent identity: range1588510
display id: range1588510
version: 1
direction: direction/forward
top level: BBa_I13457
start: 1
end: 21
orientation: inline

BBa_J37019
type: ComponentDefinition
persistent identity: BBa J37019
display id: BBa_J37019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL induced LuxR generator
creator: Deepti Aswani
created: 2006-07-18T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
group u list: _66_
m user id: 0
owner id: 1064
owning group id: 66
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0062
component: BBa_C0062
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_J37019_sequence/1

BBa_R0062
type: Component
persistent identity: component2245214
display id: component2245214
version: 1
top level: BBa_J37019
access: public
definition: lux pR

BBa_C0062
type: Component
persistent identity: component2245223
display id: component2245223
version: 1
top level: BBa_J37019
access: public
definition: luxr

BBa_B0034
type: Component
persistent identity: component2245220
display id: component2245220
version: 1
top level: BBa_J37019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2245220
display id: annotation2245220
version: 1
top level: BBa_J37019
location: https://synbiohub.org/public/igem/BBa_J37019/annotation2245220/range2245220/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J37019/annotation2245220/range2245220/1
type: Range
persistent identity: range2245220
display id: range2245220
version: 1
direction: direction/reverse
top level: BBa_J37019
start: 64
end: 75
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2245223
display id: annotation2245223
version: 1
top level: BBa_J37019
location: https://synbiohub.org/public/igem/BBa_J37019/annotation2245223/range2245223/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_J37019/annotation2245223/range2245223/1
type: Range
persistent identity: range2245223
display id: range2245223
version: 1
direction: direction/forward
top level: BBa_J37019
start: 82
end: 837
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2245214
display id: annotation2245214
version: 1
top level: BBa_J37019
location: https://synbiohub.org/public/igem/BBa_J37019/annotation2245214/range2245214/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J37019/annotation2245214/range2245214/1
type: Range
persistent identity: range2245214
display id: range2245214
version: 1
direction: direction/forward
top level: BBa_J37019
start: 1
end: 55
orientation: inline

BBa_M30011
type: ComponentDefinition
persistent identity: BBa M30011
display id: BBa_M30011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR controlled mRFP
creator: natalie kuldell
created: 2005-12-14T12:00:00Z
modified: 2015-09-17T06:41:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _45_1_
m user id: 25546
owner id: 314
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of mRFP (I13507). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. An alternative clone is M30012. Cells bearing this construct are pale pink.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0082
component: BBa_B0034
component: BBa_E1010
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_R0082
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_M30011_sequence/1

BBa_R0082
type: Component
persistent identity: component1768968
display id: component1768968
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0034
type: Component
persistent identity: component1768973
display id: component1768973
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1768980
display id: component1768980
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1768986
display id: component1768986
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1768996
display id: component1768996
version: 1
top level: BBa_M30011
access: public
definition: BBa_B0012

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1768996
display id: annotation1768996
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768996/range1768996/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30011/annotation1768996/range1768996/1
type: Range
persistent identity: range1768996
display id: range1768996
version: 1
direction: direction/forward
top level: BBa_M30011
start: 937
end: 977
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1768986
display id: annotation1768986
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768986/range1768986/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30011/annotation1768986/range1768986/1
type: Range
persistent identity: range1768986
display id: range1768986
version: 1
direction: direction/forward
top level: BBa_M30011
start: 849
end: 928
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1768980
display id: annotation1768980
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768980/range1768980/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M30011/annotation1768980/range1768980/1
type: Range
persistent identity: range1768980
display id: range1768980
version: 1
direction: direction/forward
top level: BBa_M30011
start: 135
end: 815
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1768968
display id: annotation1768968
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768968/range1768968/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M30011/annotation1768968/range1768968/1
type: Range
persistent identity: range1768968
display id: range1768968
version: 1
direction: direction/forward
top level: BBa_M30011
start: 1
end: 108
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1768973
display id: annotation1768973
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768973/range1768973/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30011/annotation1768973/range1768973/1
type: Range
persistent identity: range1768973
display id: range1768973
version: 1
direction: direction/forward
top level: BBa_M30011
start: 117
end: 128
orientation: inline

BBa_R2109
type: ComponentDefinition
persistent identity: BBa R2109
display id: BBa_R2109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: Placement of the operator site was determined to optimize steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 site
sequence annotation: -35 site
sequence annotation: transcription start site
sequence annotation: operator site for C2003
sequence: https://synbiohub.org/public/igem/BBa_R2109_sequence/1

-10 site
type: SequenceAnnotation
persistent identity: annotation1903913
display id: annotation1903913
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1
type: Range
persistent identity: range1903913
display id: range1903913
version: 1
direction: direction/forward
top level: BBa_R2109
start: 40
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903912
display id: annotation1903912
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1
type: Range
persistent identity: range1903912
display id: range1903912
version: 1
direction: direction/forward
top level: BBa_R2109
start: 17
end: 22
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903914
display id: annotation1903914
version: 1
top level: BBa_R2109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1
type: Range
persistent identity: range1903914
display id: range1903914
version: 1
direction: direction/forward
top level: BBa_R2109
start: 53
end: 53
orientation: inline

operator site for C2003
type: SequenceAnnotation
persistent identity: annotation1903915
display id: annotation1903915
version: 1
top level: BBa_R2109
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1
type: Range
persistent identity: range1903915
display id: range1903915
version: 1
direction: direction/forward
top level: BBa_R2109
start: 22
end: 33
orientation: inline

BBa_K567015
type: ComponentDefinition
persistent identity: BBa K567015
display id: BBa_K567015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-metGN
creator: You Wang
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator. We have cloned metG from E.coli and the tRNA recognition domain of metG is truncated. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and metY-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LBKana plate.
mutable notes: overlapping PCR is used
mutable provenance: metG from E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: MetRS(truncated)
sequence annotation: T7 promoter
sequence annotation: T7 TE
sequence annotation: rbs
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K567015_sequence/1

MetRS(truncated)
type: SequenceAnnotation
persistent identity: annotation2153247
display id: annotation2153247
version: 1
top level: BBa_K567015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2153247/range2153247/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2153247/range2153247/1
type: Range
persistent identity: range2153247
display id: range2153247
version: 1
direction: direction/forward
top level: BBa_K567015
start: 89
end: 1219
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2150829
display id: annotation2150829
version: 1
top level: BBa_K567015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150829/range2150829/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150829/range2150829/1
type: Range
persistent identity: range2150829
display id: range2150829
version: 1
direction: direction/forward
top level: BBa_K567015
start: 2
end: 18
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2153277
display id: annotation2153277
version: 1
top level: BBa_K567015
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2153277/range2153277/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2153277/range2153277/1
type: Range
persistent identity: range2153277
display id: range2153277
version: 1
direction: direction/forward
top level: BBa_K567015
start: 1389
end: 1436
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150831
display id: annotation2150831
version: 1
top level: BBa_K567015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150831/range2150831/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150831/range2150831/1
type: Range
persistent identity: range2150831
display id: range2150831
version: 1
direction: direction/forward
top level: BBa_K567015
start: 75
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150830
display id: annotation2150830
version: 1
top level: BBa_K567015
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150830/range2150830/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150830/range2150830/1
type: Range
persistent identity: range2150830
display id: range2150830
version: 1
direction: direction/forward
top level: BBa_K567015
start: 21
end: 44
orientation: inline

BBa_K773002
type: ComponentDefinition
persistent identity: BBa K773002
display id: BBa_K773002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Proteorhodopsin
creator: Katie Knister
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part carries the gene for a proteorhodopsin pump, which can generate a proton motive force for making ATP.
mutable notes: We codon optimized the gene for E. coli and eliminated four restriction sites.
mutable provenance: Synthesized with gene assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Proteorhodopsin
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K773002_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2209515
display id: annotation2209515
version: 1
top level: BBa_K773002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K773002/annotation2209515/range2209515/1

https://synbiohub.org/public/igem/BBa_K773002/annotation2209515/range2209515/1
type: Range
persistent identity: range2209515
display id: range2209515
version: 1
direction: direction/forward
top level: BBa_K773002
start: 1
end: 3
orientation: inline

Proteorhodopsin
type: SequenceAnnotation
persistent identity: annotation2209514
display id: annotation2209514
version: 1
top level: BBa_K773002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K773002/annotation2209514/range2209514/1

https://synbiohub.org/public/igem/BBa_K773002/annotation2209514/range2209514/1
type: Range
persistent identity: range2209514
display id: range2209514
version: 1
direction: direction/forward
top level: BBa_K773002
start: 1
end: 777
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2209516
display id: annotation2209516
version: 1
top level: BBa_K773002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773002/annotation2209516/range2209516/1

https://synbiohub.org/public/igem/BBa_K773002/annotation2209516/range2209516/1
type: Range
persistent identity: range2209516
display id: range2209516
version: 1
direction: direction/forward
top level: BBa_K773002
start: 775
end: 777
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2209517
display id: annotation2209517
version: 1
top level: BBa_K773002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773002/annotation2209517/range2209517/1

https://synbiohub.org/public/igem/BBa_K773002/annotation2209517/range2209517/1
type: Range
persistent identity: range2209517
display id: range2209517
version: 1
direction: direction/forward
top level: BBa_K773002
start: 772
end: 774
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0030.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
direction: direction/forward
top level: GFP genera
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
direction: direction/forward
top level: GFP genera
start: 22
end: 741
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
direction: direction/forward
top level: GFP genera
start: 838
end: 878
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
direction: direction/forward
top level: GFP genera
start: 750
end: 829
orientation: inline

BBa_K775002
type: ComponentDefinition
persistent identity: BBa K775002
display id: BBa_K775002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-OR1G1 Isoamylacetate chimeric GPCR
creator: Sietske Grijseels
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775002 encodes a chimeric isoamylacetate-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mouse OR1G1, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: -
mutable provenance: N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: ORG1, (Homo sapiens) NM_003555 C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K775002_sequence/1

BBa_K812020
type: ComponentDefinition
persistent identity: BBa K812020
display id: BBa_K812020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eukaryotic PoPs -> IaaH. Indoleacetamide hydrolyase for Auxin generator, with kozak
creator: William Rostain
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: true
group u list: _1069_
m user id: 0
owner id: 7053
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains the coding sequence for Indoleacetamide hydrolyase preceded by a Kozak sequence, for expression in eukaryotic plasmids.
mutable notes: Kozak is included, as it cannot be added by biobrick. This part can be used to assemble biobricks for integration onto Xenopus tropicalis genome. It can also be used directly by injection into a fertilised egg, using the ready to use Xenopus plasmid BBa_K812000.
mutable provenance: This part was created from BBa_K515100, itself from P. savastanoi (By Imperial college)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IaaH
sequence annotation: Kozak sequence
sequence: https://synbiohub.org/public/igem/BBa_K812020_sequence/1

IaaH
type: SequenceAnnotation
persistent identity: annotation2187776
display id: annotation2187776
version: 1
top level: BBa_K812020
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812020/annotation2187776/range2187776/1

https://synbiohub.org/public/igem/BBa_K812020/annotation2187776/range2187776/1
type: Range
persistent identity: range2187776
display id: range2187776
version: 1
direction: direction/forward
top level: BBa_K812020
start: 12
end: 1355
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2187775
display id: annotation2187775
version: 1
top level: BBa_K812020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K812020/annotation2187775/range2187775/1

https://synbiohub.org/public/igem/BBa_K812020/annotation2187775/range2187775/1
type: Range
persistent identity: range2187775
display id: range2187775
version: 1
direction: direction/forward
top level: BBa_K812020
start: 1
end: 11
orientation: inline

BBa_K737032
type: ComponentDefinition
persistent identity: BBa K737032
display id: BBa_K737032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spot42 based small RNA2
creator: Peiran Zhang and Minghao Gong
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose operon and other sugar metabolic pathway,such as galK,nanC,ytfJ,srlA,which are the 5??? leader sequence of the corresponding metabolic enzymes. Take galK for example,Spot42 causes translation repression by base pairing to RBS in the 5??? leader sequence,then block the recognition of the antiSD sequence on 30S subunit. It???s a very strong pairing(up to 20bases),but it only causes 2.6 fold repression according to Johannes H. Urban and Jo?? rg Vogel,for it doesn???t result in the degradation of the RNA complex by RNaseE(ssRNA degradation) and RNaseIII(dsRNA degradation),both of them are major enzymes that causes RNA degradation in vivo.Spot42-galK complex degrades in a slow and presently unclear way. The base pairing is initiated by the recognition of seed region,which plays a significant role in the repression efficiency.
mutable notes: No
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K737032_sequence/1

PCaa3
type: ComponentDefinition
persistent identity: BBa K743002
display id: BBa_K743002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PCaa3 promoter and native rbs from Synechocystis PCC6803
creator: Juan Sim??n ??lamos
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the 100bp sequence upstream the Caa3 gene start codon, it contains putative rbs and promoter regions. Caa3 codes for a putative cytochrome aa3 controlling protein and its mRNA levels have shown to oscillate in a circadian manner, peaking at hour 9.
mutable notes: the desition of taking just 100bp upstream C
mutable provenance: this part was PCR amplified from Synechocystis chromosome, orf sll1898.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pcaa3 promoter
sequence: https://synbiohub.org/public/igem/BBa_K743002_sequence/1

Pcaa3 promoter
type: SequenceAnnotation
persistent identity: annotation2188681
display id: annotation2188681
version: 1
top level: PCaa3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K743002/annotation2188681/range2188681/1

https://synbiohub.org/public/igem/BBa_K743002/annotation2188681/range2188681/1
type: Range
persistent identity: range2188681
display id: range2188681
version: 1
direction: direction/forward
top level: PCaa3
start: 1
end: 101
orientation: inline

BBa_K747053
type: ComponentDefinition
persistent identity: BBa K747053
display id: BBa_K747053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 4
sequence: https://synbiohub.org/public/igem/BBa_K747053_sequence/1

CC 4
type: SequenceAnnotation
persistent identity: annotation2196693
display id: annotation2196693
version: 1
top level: BBa_K747053
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747053/annotation2196693/range2196693/1

https://synbiohub.org/public/igem/BBa_K747053/annotation2196693/range2196693/1
type: Range
persistent identity: range2196693
display id: range2196693
version: 1
direction: direction/forward
top level: BBa_K747053
start: 14
end: 211
orientation: inline

BBa_J01141
type: ComponentDefinition
persistent identity: BBa J01141
display id: BBa_J01141
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [pLacI pL][key1]
creator: Golden Bear (Berkeley iGEM 2005)
created: 2006-04-13T11:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_1_
m user id: 0
owner id: 612
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_R0011
component: BBa_J01008
sequence annotation: BBa_R0011
sequence annotation: BBa_J01008
sequence: https://synbiohub.org/public/igem/BBa_J01141_sequence/1

BBa_R0011
type: Component
persistent identity: component1843682
display id: component1843682
version: 1
top level: BBa_J01141
access: public
definition: lacI+pL

BBa_J01008
type: Component
persistent identity: component1843688
display id: component1843688
version: 1
top level: BBa_J01141
access: public
definition: https://synbiohub.org/public/igem/BBa_J01008/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1843682
display id: annotation1843682
version: 1
top level: BBa_J01141
location: https://synbiohub.org/public/igem/BBa_J01141/annotation1843682/range1843682/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J01141/annotation1843682/range1843682/1
type: Range
persistent identity: range1843682
display id: range1843682
version: 1
direction: direction/forward
top level: BBa_J01141
start: 1
end: 54
orientation: inline

BBa_J01008
type: SequenceAnnotation
persistent identity: annotation1843688
display id: annotation1843688
version: 1
top level: BBa_J01141
location: https://synbiohub.org/public/igem/BBa_J01141/annotation1843688/range1843688/1
component: BBa_J01008

https://synbiohub.org/public/igem/BBa_J01141/annotation1843688/range1843688/1
type: Range
persistent identity: range1843688
display id: range1843688
version: 1
direction: direction/reverse
top level: BBa_J01141
start: 64
end: 157
orientation: inline

BBa_K936020
type: ComponentDefinition
persistent identity: BBa K936020
display id: BBa_K936020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K206000+B0034+pelB+Cutinase+Polyhistidine Tag
creator: Mattan Hamou
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
group u list: _1201_
m user id: 0
owner id: 13899
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Full Cutinase Construct on psB1A3 backbone.
mutable notes: There's no terminator so it needs to be used with a backbone that ends with a 3
mutable provenance: http://www.google.com/url
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K936014
component: BBa_K936015
sequence annotation: BBa_K936014
sequence annotation: BBa_K936015
sequence: https://synbiohub.org/public/igem/BBa_K936020_sequence/1

BBa_K936014
type: Component
persistent identity: component2188714
display id: component2188714
version: 1
top level: BBa_K936020
access: public
definition: https://synbiohub.org/public/igem/BBa_K936014/1

BBa_K936015
type: Component
persistent identity: component2188710
display id: component2188710
version: 1
top level: BBa_K936020
access: public
definition: https://synbiohub.org/public/igem/BBa_K936015/1

BBa_K936014
type: SequenceAnnotation
persistent identity: annotation2188714
display id: annotation2188714
version: 1
top level: BBa_K936020
location: https://synbiohub.org/public/igem/BBa_K936020/annotation2188714/range2188714/1
component: BBa_K936014

https://synbiohub.org/public/igem/BBa_K936020/annotation2188714/range2188714/1
type: Range
persistent identity: range2188714
display id: range2188714
version: 1
direction: direction/reverse
top level: BBa_K936020
start: 157
end: 1125
orientation: inline

BBa_K936015
type: SequenceAnnotation
persistent identity: annotation2188710
display id: annotation2188710
version: 1
top level: BBa_K936020
location: https://synbiohub.org/public/igem/BBa_K936020/annotation2188710/range2188710/1
component: BBa_K936015

https://synbiohub.org/public/igem/BBa_K936020/annotation2188710/range2188710/1
type: Range
persistent identity: range2188710
display id: range2188710
version: 1
direction: direction/reverse
top level: BBa_K936020
start: 1
end: 150
orientation: inline

BBa_K747051
type: ComponentDefinition
persistent identity: BBa K747051
display id: BBa_K747051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 4
sequence: https://synbiohub.org/public/igem/BBa_K747051_sequence/1

AT 4
type: SequenceAnnotation
persistent identity: annotation2196280
display id: annotation2196280
version: 1
top level: BBa_K747051
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747051/annotation2196280/range2196280/1

https://synbiohub.org/public/igem/BBa_K747051/annotation2196280/range2196280/1
type: Range
persistent identity: range2196280
display id: range2196280
version: 1
direction: direction/forward
top level: BBa_K747051
start: 14
end: 211
orientation: inline

BBa_K731250
type: ComponentDefinition
persistent identity: BBa K731250
display id: BBa_K731250
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible araC-pBAD promoter with GFPmut3b, strong RBS
creator: Daniele Rossetto
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200). The part is composed of part BBa_K731200 and BBa_E0840
mutable provenance: araC-pBAD from E. coli genome, GFPmut3b is from the registry (part BBa_E0840)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0240
component: BBa_K731201
sequence annotation: BBa_K731201
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K731250_sequence/1

BBa_E0240
type: Component
persistent identity: component2331165
display id: component2331165
version: 1
top level: BBa_K731250
access: public
definition: GFP report

BBa_K731201
type: Component
persistent identity: component2331154
display id: component2331154
version: 1
top level: BBa_K731250
access: public
definition: https://synbiohub.org/public/igem/BBa_K731201/1

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2331154
display id: annotation2331154
version: 1
top level: BBa_K731250
location: https://synbiohub.org/public/igem/BBa_K731250/annotation2331154/range2331154/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K731250/annotation2331154/range2331154/1
type: Range
persistent identity: range2331154
display id: range2331154
version: 1
direction: direction/reverse
top level: BBa_K731250
start: 1
end: 1203
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2331165
display id: annotation2331165
version: 1
top level: BBa_K731250
location: https://synbiohub.org/public/igem/BBa_K731250/annotation2331165/range2331165/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K731250/annotation2331165/range2331165/1
type: Range
persistent identity: range2331165
display id: range2331165
version: 1
direction: direction/reverse
top level: BBa_K731250
start: 1212
end: 2087
orientation: inline

BBa_K911003
type: ComponentDefinition
persistent identity: BBa K911003
display id: BBa_K911003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluoride Sensitive Riboswitch
creator: Oliver Meacock
created: 2012-09-11T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1176_
m user id: 0
owner id: 12694
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Riboswitch that is highly sensitive to the F- ion. We have characterized this part in three different chassis: TOP10 e.coli, Laboratory strain bacillus subtilis and a strain of bacillus subtilis with its normal fluoride riboswitch knocked out (kindly provided by the Breaker lab in Yale). Results of Miller assays for these three chassis are shown below.
mutable notes: Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter, in this case lacZ as our reporter.
mutable provenance: Bacillus Cereus Genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: Lysine promoter
sequence annotation: Fluoride riboswitch
sequence: https://synbiohub.org/public/igem/BBa_K911003_sequence/1

Lysine promoter
type: SequenceAnnotation
persistent identity: annotation2182732
display id: annotation2182732
version: 1
top level: BBa_K911003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K911003/annotation2182732/range2182732/1

https://synbiohub.org/public/igem/BBa_K911003/annotation2182732/range2182732/1
type: Range
persistent identity: range2182732
display id: range2182732
version: 1
direction: direction/forward
top level: BBa_K911003
start: 1
end: 43
orientation: inline

Fluoride riboswitch
type: SequenceAnnotation
persistent identity: annotation2182733
display id: annotation2182733
version: 1
top level: BBa_K911003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K911003/annotation2182733/range2182733/1

https://synbiohub.org/public/igem/BBa_K911003/annotation2182733/range2182733/1
type: Range
persistent identity: range2182733
display id: range2182733
version: 1
direction: direction/forward
top level: BBa_K911003
start: 61
end: 139
orientation: inline

BBa_K747029
type: ComponentDefinition
persistent identity: BBa K747029
display id: BBa_K747029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 2
sequence: https://synbiohub.org/public/igem/BBa_K747029_sequence/1

TC 2
type: SequenceAnnotation
persistent identity: annotation2196201
display id: annotation2196201
version: 1
top level: BBa_K747029
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747029/annotation2196201/range2196201/1

https://synbiohub.org/public/igem/BBa_K747029/annotation2196201/range2196201/1
type: Range
persistent identity: range2196201
display id: range2196201
version: 1
direction: direction/forward
top level: BBa_K747029
start: 14
end: 211
orientation: inline

BBa_K861042
type: ComponentDefinition
persistent identity: BBa K861042
display id: BBa_K861042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FadA, gene for fatty acid degradation from Salmonella enterica LT2
creator: Kuanwei Sheng
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FadA gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadA have far better efficiency compared to FadA in E.coli.(???S-??? is used to distinguish FadA gene in E.coli )
mutable notes: None
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K861042_sequence/1

BBa_K784013
type: ComponentDefinition
persistent identity: BBa K784013
display id: BBa_K784013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux+Theophylline riboswitch+mCherry
creator: Ilya Vainberg Slutskin
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: true
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequence is the product of restriction cloning of the theophylline riboswitch downstream to the pLux promoter. In the presence of LuxR transcription can be induced by 3OC6HSL.
mutable notes: No design considerations.
mutable provenance: Cloning of theophylline riboswitch downstream to the pLux promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K784006
sequence annotation: BBa_R0062
sequence annotation: BBa_K784006
sequence: https://synbiohub.org/public/igem/BBa_K784013_sequence/1

BBa_R0062
type: Component
persistent identity: component2183682
display id: component2183682
version: 1
top level: BBa_K784013
access: public
definition: lux pR

BBa_K784006
type: Component
persistent identity: component2183694
display id: component2183694
version: 1
top level: BBa_K784013
access: public
definition: BBa_K784006

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2183682
display id: annotation2183682
version: 1
top level: BBa_K784013
location: https://synbiohub.org/public/igem/BBa_K784013/annotation2183682/range2183682/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K784013/annotation2183682/range2183682/1
type: Range
persistent identity: range2183682
display id: range2183682
version: 1
direction: direction/forward
top level: BBa_K784013
start: 1
end: 55
orientation: inline

BBa_K784006
type: SequenceAnnotation
persistent identity: annotation2183694
display id: annotation2183694
version: 1
top level: BBa_K784013
location: https://synbiohub.org/public/igem/BBa_K784013/annotation2183694/range2183694/1
component: BBa_K784006

https://synbiohub.org/public/igem/BBa_K784013/annotation2183694/range2183694/1
type: Range
persistent identity: range2183694
display id: range2183694
version: 1
direction: direction/reverse
top level: BBa_K784013
start: 64
end: 851
orientation: inline

BBa_S0100
type: ComponentDefinition
persistent identity: BBa S0100
display id: BBa_S0100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0012
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0012
component: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S0100_sequence/1

BBa_C0012
type: Component
persistent identity: component939708
display id: component939708
version: 1
top level: BBa_S0100
access: public
definition: lacI

BBa_B0034
type: Component
persistent identity: component939698
display id: component939698
version: 1
top level: BBa_S0100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation939708
display id: annotation939708
version: 1
top level: BBa_S0100
location: https://synbiohub.org/public/igem/BBa_S0100/annotation939708/range939708/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_S0100/annotation939708/range939708/1
type: Range
persistent identity: range939708
display id: range939708
version: 1
direction: direction/forward
top level: BBa_S0100
start: 19
end: 1146
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939698
display id: annotation939698
version: 1
top level: BBa_S0100
location: https://synbiohub.org/public/igem/BBa_S0100/annotation939698/range939698/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0100/annotation939698/range939698/1
type: Range
persistent identity: range939698
display id: range939698
version: 1
direction: direction/forward
top level: BBa_S0100
start: 1
end: 12
orientation: inline

BBa_K876052
type: ComponentDefinition
persistent identity: BBa K876052
display id: BBa_K876052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plasR-luxI-RBS-YFP-Term-Term
creator: Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pLasR is off by default and can be actated by LasR. LuxI converts SAM to AHL.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0079
component: BBa_C0061
component: BBa_E0432
sequence annotation: BBa_E0432
sequence annotation: BBa_C0061
sequence annotation: BBa_R0079
sequence: https://synbiohub.org/public/igem/BBa_K876052_sequence/1

BBa_R0079
type: Component
persistent identity: component2264725
display id: component2264725
version: 1
top level: BBa_K876052
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_C0061
type: Component
persistent identity: component2264727
display id: component2264727
version: 1
top level: BBa_K876052
access: public
definition: luxI

BBa_E0432
type: Component
persistent identity: component2264743
display id: component2264743
version: 1
top level: BBa_K876052
access: public
definition: https://synbiohub.org/public/igem/BBa_E0432/1

BBa_E0432
type: SequenceAnnotation
persistent identity: annotation2264743
display id: annotation2264743
version: 1
top level: BBa_K876052
location: https://synbiohub.org/public/igem/BBa_K876052/annotation2264743/range2264743/1
component: BBa_E0432

https://synbiohub.org/public/igem/BBa_K876052/annotation2264743/range2264743/1
type: Range
persistent identity: range2264743
display id: range2264743
version: 1
direction: direction/reverse
top level: BBa_K876052
start: 815
end: 1731
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2264727
display id: annotation2264727
version: 1
top level: BBa_K876052
location: https://synbiohub.org/public/igem/BBa_K876052/annotation2264727/range2264727/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876052/annotation2264727/range2264727/1
type: Range
persistent identity: range2264727
display id: range2264727
version: 1
direction: direction/forward
top level: BBa_K876052
start: 164
end: 781
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2264725
display id: annotation2264725
version: 1
top level: BBa_K876052
location: https://synbiohub.org/public/igem/BBa_K876052/annotation2264725/range2264725/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K876052/annotation2264725/range2264725/1
type: Range
persistent identity: range2264725
display id: range2264725
version: 1
direction: direction/reverse
top level: BBa_K876052
start: 1
end: 157
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: monomeric RFP optimized for bacteria
creator: ytwang
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
direction: direction/forward
top level: mCherry
start: 352
end: 352
orientation: inline

BBa_K876013
type: ComponentDefinition
persistent identity: BBa K876013
display id: BBa_K876013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-RFP-ST-pLac-LuxI
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI and RFP attached to a constitutive promoter for LuxI
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_K081014
component: BBa_C0012
component: BBa_C0061
sequence annotation: BBa_C0061
sequence annotation: BBa_R0010
sequence annotation: BBa_C0012
sequence annotation: BBa_K081014
sequence: https://synbiohub.org/public/igem/BBa_K876013_sequence/1

BBa_R0010
type: Component
persistent identity: component2264696
display id: component2264696
version: 1
top level: BBa_K876013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K081014
type: Component
persistent identity: component2264695
display id: component2264695
version: 1
top level: BBa_K876013
access: public
definition: https://synbiohub.org/public/igem/BBa_K081014/1

BBa_C0012
type: Component
persistent identity: component2264680
display id: component2264680
version: 1
top level: BBa_K876013
access: public
definition: lacI

BBa_C0061
type: Component
persistent identity: component2264704
display id: component2264704
version: 1
top level: BBa_K876013
access: public
definition: luxI

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2264704
display id: annotation2264704
version: 1
top level: BBa_K876013
location: https://synbiohub.org/public/igem/BBa_K876013/annotation2264704/range2264704/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K876013/annotation2264704/range2264704/1
type: Range
persistent identity: range2264704
display id: range2264704
version: 1
direction: direction/forward
top level: BBa_K876013
start: 2150
end: 2767
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2264696
display id: annotation2264696
version: 1
top level: BBa_K876013
location: https://synbiohub.org/public/igem/BBa_K876013/annotation2264696/range2264696/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K876013/annotation2264696/range2264696/1
type: Range
persistent identity: range2264696
display id: range2264696
version: 1
direction: direction/forward
top level: BBa_K876013
start: 1944
end: 2143
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2264680
display id: annotation2264680
version: 1
top level: BBa_K876013
location: https://synbiohub.org/public/igem/BBa_K876013/annotation2264680/range2264680/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K876013/annotation2264680/range2264680/1
type: Range
persistent identity: range2264680
display id: range2264680
version: 1
direction: direction/forward
top level: BBa_K876013
start: 1
end: 1128
orientation: inline

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2264695
display id: annotation2264695
version: 1
top level: BBa_K876013
location: https://synbiohub.org/public/igem/BBa_K876013/annotation2264695/range2264695/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K876013/annotation2264695/range2264695/1
type: Range
persistent identity: range2264695
display id: range2264695
version: 1
direction: direction/reverse
top level: BBa_K876013
start: 1162
end: 1935
orientation: inline

BBa_K864444
type: ComponentDefinition
persistent identity: BBa K864444
display id: BBa_K864444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-Linker-SYFP2
creator: Joel ??s
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 13998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Use this part to insert any 5?????TR of your gene of interest to screen for downregulation of your gene with small RNA. PCR amplify your 5???UTR with EcoRI and BamHI and clone into any backbone carrying this part. Digest whis part with EcoRI and BamHI and as RFP is replaced, this part functions as a red/white screening system. This allows you to create a reporter system with a fluorescent marker for screening for functional small RNAs. SYFP2 BBa_K864100 is situated downstrean of the RFP BBa_J04450 and is connected in frame with a 12 aa glycine-serine repeated linker BBa_J18922 , to prevent folding distruptions in the YFP or the 5???UTR. The first 6 nt of the glycine-serine linker is a BamHI restriction site (GGATCC), which translates into glycine-serine in E.Coli. When screening for working small RNAs, it's recommended to include the first 15 codons of the gene of interest.
mutable notes: -
mutable provenance: Constructed from SYFP2 BBa_K864100 , linker BBa_J18922 and RFP BBa_J04450
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RFP
sequence annotation: B0012
sequence annotation: RFP J04450
sequence annotation: Plac
sequence annotation: SYFP2
sequence annotation: BamHI
sequence annotation: B0034
sequence annotation: Linker
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_K864444_sequence/1

RFP
type: SequenceAnnotation
persistent identity: annotation2198887
display id: annotation2198887
version: 1
top level: BBa_K864444
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198887/range2198887/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198887/range2198887/1
type: Range
persistent identity: range2198887
display id: range2198887
version: 1
direction: direction/forward
top level: BBa_K864444
start: 227
end: 681
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation2198889
display id: annotation2198889
version: 1
top level: BBa_K864444
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198889/range2198889/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198889/range2198889/1
type: Range
persistent identity: range2198889
display id: range2198889
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1029
end: 1069
orientation: inline

RFP J04450
type: SequenceAnnotation
persistent identity: annotation2198883
display id: annotation2198883
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198883/range2198883/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198883/range2198883/1
type: Range
persistent identity: range2198883
display id: range2198883
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1
end: 1068
orientation: inline

Plac
type: SequenceAnnotation
persistent identity: annotation2198885
display id: annotation2198885
version: 1
top level: BBa_K864444
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198885/range2198885/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198885/range2198885/1
type: Range
persistent identity: range2198885
display id: range2198885
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1
end: 200
orientation: inline

SYFP2
type: SequenceAnnotation
persistent identity: annotation2198896
display id: annotation2198896
version: 1
top level: BBa_K864444
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198896/range2198896/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198896/range2198896/1
type: Range
persistent identity: range2198896
display id: range2198896
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1106
end: 1825
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2198894
display id: annotation2198894
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198894/range2198894/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198894/range2198894/1
type: Range
persistent identity: range2198894
display id: range2198894
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1070
end: 1075
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2198886
display id: annotation2198886
version: 1
top level: BBa_K864444
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198886/range2198886/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198886/range2198886/1
type: Range
persistent identity: range2198886
display id: range2198886
version: 1
direction: direction/forward
top level: BBa_K864444
start: 209
end: 220
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198893
display id: annotation2198893
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198893/range2198893/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198893/range2198893/1
type: Range
persistent identity: range2198893
display id: range2198893
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1076
end: 1105
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation2198890
display id: annotation2198890
version: 1
top level: BBa_K864444
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198890/range2198890/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198890/range2198890/1
type: Range
persistent identity: range2198890
display id: range2198890
version: 1
direction: direction/forward
top level: BBa_K864444
start: 941
end: 1020
orientation: inline

BBa_K808032
type: ComponentDefinition
persistent identity: BBa K808032
display id: BBa_K808032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
creator: Marie Burghard, Jascha Diemer, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-03T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 12529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This a a composite of 2 different BioBricks by an standard assembly, forming a scar in between. It contains the following units: AraC-AraO2-AraO1-AraPromo(PBAD)- RBS-PhoA-His6tag-pNBEst13-Myctag-EstA which is similiar to BBa_K808000 & BBa_K808030 Its a regulatory system, being induced by adding 1 to 2% of mw% L-Arabinose. It is made for regulating the cell surface Expression of our PET cleaving enzyme pNB-Est13, which is anchored c-terminal to EstA.
mutable notes: We were not able to add this part as an composite due to missing links and unedited registry pages. Necessary informations are featured in the sequence.
mutable provenance: assembled GENEART synthesized product(BBa_K808030)and cloned regulatory unit (BBa_K808000), deriving from an expression system (pBAD-vector)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K808026
sequence annotation: BBa_K808028
sequence annotation: myctag
sequence annotation: ara O2
sequence annotation: AraC
sequence annotation: stop
sequence annotation: BBa_K808027
sequence annotation: BBa_B0029
sequence annotation: scar
sequence annotation: ara O1
sequence annotation: 6histag
sequence annotation: ara promotor
sequence: https://synbiohub.org/public/igem/BBa_K808032_sequence/1

BBa_K808026
type: SequenceAnnotation
persistent identity: annotation2183172
display id: annotation2183172
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183172/range2183172/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183172/range2183172/1
type: Range
persistent identity: range2183172
display id: range2183172
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1322
end: 2791
orientation: inline

BBa_K808028
type: SequenceAnnotation
persistent identity: annotation2183171
display id: annotation2183171
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183171/range2183171/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183171/range2183171/1
type: Range
persistent identity: range2183171
display id: range2183171
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1241
end: 1303
orientation: inline

myctag
type: SequenceAnnotation
persistent identity: annotation2183173
display id: annotation2183173
version: 1
top level: BBa_K808032
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183173/range2183173/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183173/range2183173/1
type: Range
persistent identity: range2183173
display id: range2183173
version: 1
direction: direction/forward
top level: BBa_K808032
start: 2792
end: 2821
orientation: inline

ara O2
type: SequenceAnnotation
persistent identity: annotation2183165
display id: annotation2183165
version: 1
top level: BBa_K808032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183165/range2183165/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183165/range2183165/1
type: Range
persistent identity: range2183165
display id: range2183165
version: 1
direction: direction/forward
top level: BBa_K808032
start: 909
end: 924
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2183164
display id: annotation2183164
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183164/range2183164/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183164/range2183164/1
type: Range
persistent identity: range2183164
display id: range2183164
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1
end: 879
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2183176
display id: annotation2183176
version: 1
top level: BBa_K808032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183176/range2183176/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183176/range2183176/1
type: Range
persistent identity: range2183176
display id: range2183176
version: 1
direction: direction/forward
top level: BBa_K808032
start: 4259
end: 4261
orientation: inline

BBa_K808027
type: SequenceAnnotation
persistent identity: annotation2183174
display id: annotation2183174
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183174/range2183174/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183174/range2183174/1
type: Range
persistent identity: range2183174
display id: range2183174
version: 1
direction: direction/forward
top level: BBa_K808032
start: 2822
end: 4255
orientation: inline

BBa_B0029
type: SequenceAnnotation
persistent identity: annotation2183169
display id: annotation2183169
version: 1
top level: BBa_K808032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183169/range2183169/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183169/range2183169/1
type: Range
persistent identity: range2183169
display id: range2183169
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1220
end: 1234
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2183175
display id: annotation2183175
version: 1
top level: BBa_K808032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183175/range2183175/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183175/range2183175/1
type: Range
persistent identity: range2183175
display id: range2183175
version: 1
direction: direction/forward
top level: BBa_K808032
start: 4256
end: 4258
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2183168
display id: annotation2183168
version: 1
top level: BBa_K808032
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183168/range2183168/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183168/range2183168/1
type: Range
persistent identity: range2183168
display id: range2183168
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1211
end: 1218
orientation: inline

ara O1
type: SequenceAnnotation
persistent identity: annotation2183166
display id: annotation2183166
version: 1
top level: BBa_K808032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183166/range2183166/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183166/range2183166/1
type: Range
persistent identity: range2183166
display id: range2183166
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1066
end: 1077
orientation: inline

6histag
type: SequenceAnnotation
persistent identity: annotation2183170
display id: annotation2183170
version: 1
top level: BBa_K808032
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183170/range2183170/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183170/range2183170/1
type: Range
persistent identity: range2183170
display id: range2183170
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1304
end: 1321
orientation: inline

ara promotor
type: SequenceAnnotation
persistent identity: annotation2183167
display id: annotation2183167
version: 1
top level: BBa_K808032
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183167/range2183167/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183167/range2183167/1
type: Range
persistent identity: range2183167
display id: range2183167
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1153
end: 1181
orientation: inline

BBa_J61051
type: ComponentDefinition
persistent identity: BBa J61051
display id: BBa_J61051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [Psal1]
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Salicylate promoter and nahR
mutable notes: nonh
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61051_sequence/1

BBa_K747057
type: ComponentDefinition
persistent identity: BBa K747057
display id: BBa_K747057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 4
sequence: https://synbiohub.org/public/igem/BBa_K747057_sequence/1

GC 4
type: SequenceAnnotation
persistent identity: annotation2197014
display id: annotation2197014
version: 1
top level: BBa_K747057
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747057/annotation2197014/range2197014/1

https://synbiohub.org/public/igem/BBa_K747057/annotation2197014/range2197014/1
type: Range
persistent identity: range2197014
display id: range2197014
version: 1
direction: direction/forward
top level: BBa_K747057
start: 14
end: 211
orientation: inline

BBa_K863011
type: ComponentDefinition
persistent identity: BBa K863011
display id: BBa_K863011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) coding sequence
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAG
sequence annotation: ATG
sequence annotation: tthl
sequence: https://synbiohub.org/public/igem/BBa_K863011_sequence/1

TAG
type: SequenceAnnotation
persistent identity: annotation2184889
display id: annotation2184889
version: 1
top level: BBa_K863011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1
type: Range
persistent identity: range2184889
display id: range2184889
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1387
end: 1389
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184806
display id: annotation2184806
version: 1
top level: BBa_K863011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1
type: Range
persistent identity: range2184806
display id: range2184806
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 3
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2184888
display id: annotation2184888
version: 1
top level: BBa_K863011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1
type: Range
persistent identity: range2184888
display id: range2184888
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 1389
orientation: inline

BBa_S05055
type: ComponentDefinition
persistent identity: BBa S05055
display id: BBa_S05055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010:P0451
creator: Muyuan Chen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:14:49Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 12559
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_P0451
component: BBa_R0010
sequence annotation: BBa_P0451
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_S05055_sequence/1

BBa_P0451
type: Component
persistent identity: component2184462
display id: component2184462
version: 1
top level: BBa_S05055
access: public
definition: https://synbiohub.org/public/igem/BBa_P0451/1

BBa_R0010
type: Component
persistent identity: component2184443
display id: component2184443
version: 1
top level: BBa_S05055
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_P0451
type: SequenceAnnotation
persistent identity: annotation2184462
display id: annotation2184462
version: 1
top level: BBa_S05055
location: https://synbiohub.org/public/igem/BBa_S05055/annotation2184462/range2184462/1
component: BBa_P0451

https://synbiohub.org/public/igem/BBa_S05055/annotation2184462/range2184462/1
type: Range
persistent identity: range2184462
display id: range2184462
version: 1
direction: direction/reverse
top level: BBa_S05055
start: 209
end: 1138
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2184443
display id: annotation2184443
version: 1
top level: BBa_S05055
location: https://synbiohub.org/public/igem/BBa_S05055/annotation2184443/range2184443/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S05055/annotation2184443/range2184443/1
type: Range
persistent identity: range2184443
display id: range2184443
version: 1
direction: direction/forward
top level: BBa_S05055
start: 1
end: 200
orientation: inline

BBa_I13528
type: ComponentDefinition
persistent identity: BBa I13528
display id: BBa_I13528
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid v1.0 intermediate
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate in screening plasmid v2 construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I13457
component: BBa_I13507
sequence annotation: BBa_I13507
sequence annotation: BBa_I13457
sequence: https://synbiohub.org/public/igem/BBa_I13528_sequence/1

BBa_I13457
type: Component
persistent identity: component2251413
display id: component2251413
version: 1
top level: BBa_I13528
access: public
definition: BBa_I13457

BBa_I13507
type: Component
persistent identity: component2251425
display id: component2251425
version: 1
top level: BBa_I13528
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2251425
display id: annotation2251425
version: 1
top level: BBa_I13528
location: https://synbiohub.org/public/igem/BBa_I13528/annotation2251425/range2251425/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_I13528/annotation2251425/range2251425/1
type: Range
persistent identity: range2251425
display id: range2251425
version: 1
direction: direction/reverse
top level: BBa_I13528
start: 120
end: 980
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation2251413
display id: annotation2251413
version: 1
top level: BBa_I13528
location: https://synbiohub.org/public/igem/BBa_I13528/annotation2251413/range2251413/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13528/annotation2251413/range2251413/1
type: Range
persistent identity: range2251413
display id: range2251413
version: 1
direction: direction/reverse
top level: BBa_I13528
start: 1
end: 111
orientation: inline

BBa_K876068
type: ComponentDefinition
persistent identity: BBa K876068
display id: BBa_K876068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Term-pLsrA-LacI
creator: Zakaraya Ashour, Sanket Shah
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: TT-pLasrA-LacI
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0012
component: BBa_K117002
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K117002
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K876068_sequence/1

BBa_C0012
type: Component
persistent identity: component2251339
display id: component2251339
version: 1
top level: BBa_K876068
access: public
definition: lacI

BBa_K117002
type: Component
persistent identity: component2251337
display id: component2251337
version: 1
top level: BBa_K876068
access: public
definition: https://synbiohub.org/public/igem/BBa_K117002/1

BBa_B0015
type: Component
persistent identity: component2251336
display id: component2251336
version: 1
top level: BBa_K876068
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251336
display id: annotation2251336
version: 1
top level: BBa_K876068
location: https://synbiohub.org/public/igem/BBa_K876068/annotation2251336/range2251336/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K876068/annotation2251336/range2251336/1
type: Range
persistent identity: range2251336
display id: range2251336
version: 1
direction: direction/reverse
top level: BBa_K876068
start: 1
end: 129
orientation: inline

BBa_K117002
type: SequenceAnnotation
persistent identity: annotation2251337
display id: annotation2251337
version: 1
top level: BBa_K876068
location: https://synbiohub.org/public/igem/BBa_K876068/annotation2251337/range2251337/1
component: BBa_K117002

https://synbiohub.org/public/igem/BBa_K876068/annotation2251337/range2251337/1
type: Range
persistent identity: range2251337
display id: range2251337
version: 1
direction: direction/reverse
top level: BBa_K876068
start: 138
end: 239
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2251339
display id: annotation2251339
version: 1
top level: BBa_K876068
location: https://synbiohub.org/public/igem/BBa_K876068/annotation2251339/range2251339/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K876068/annotation2251339/range2251339/1
type: Range
persistent identity: range2251339
display id: range2251339
version: 1
direction: direction/forward
top level: BBa_K876068
start: 246
end: 1373
orientation: inline

BBa_I732095
type: ComponentDefinition
persistent identity: BBa I732095
display id: BBa_I732095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Activity Reporter (LacZ-alpha and RFP)
creator: Zhan Jian
created: 2007-08-31T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I732074
component: BBa_I13507
sequence annotation: BBa_I13507
sequence annotation: BBa_I732074
sequence: https://synbiohub.org/public/igem/BBa_I732095_sequence/1

BBa_I732074
type: Component
persistent identity: component2265792
display id: component2265792
version: 1
top level: BBa_I732095
access: public
definition: https://synbiohub.org/public/igem/BBa_I732074/1

BBa_I13507
type: Component
persistent identity: component2265804
display id: component2265804
version: 1
top level: BBa_I732095
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2265804
display id: annotation2265804
version: 1
top level: BBa_I732095
location: https://synbiohub.org/public/igem/BBa_I732095/annotation2265804/range2265804/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_I732095/annotation2265804/range2265804/1
type: Range
persistent identity: range2265804
display id: range2265804
version: 1
direction: direction/reverse
top level: BBa_I732095
start: 288
end: 1148
orientation: inline

BBa_I732074
type: SequenceAnnotation
persistent identity: annotation2265792
display id: annotation2265792
version: 1
top level: BBa_I732095
location: https://synbiohub.org/public/igem/BBa_I732095/annotation2265792/range2265792/1
component: BBa_I732074

https://synbiohub.org/public/igem/BBa_I732095/annotation2265792/range2265792/1
type: Range
persistent identity: range2265792
display id: range2265792
version: 1
direction: direction/reverse
top level: BBa_I732095
start: 1
end: 279
orientation: inline

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c2 repressor from Salmonella phage P22 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0053
sequence annotation: stop
sequence annotation: LVA
sequence annotation: cII p22
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 687
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
direction: direction/forward
top level: c2 P22
start: 682
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
direction: direction/forward
top level: c2 P22
start: 649
end: 681
orientation: inline

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 648
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
direction: direction/forward
top level: c2 P22
start: 688
end: 712
orientation: inline

BBa_K737031
type: ComponentDefinition
persistent identity: BBa K737031
display id: BBa_K737031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spot42 based small RNA1
creator: Peiran Zhang and Minghao Gong
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose operon and other sugar metabolic pathway,such as galK,nanC,ytfJ,srlA,which are the 5??? leader sequence of the corresponding metabolic enzymes. Take galK for example,Spot42 causes translation repression by base pairing to RBS in the 5??? leader sequence,then block the recognition of the antiSD sequence on 30S subunit. It???s a very strong pairing(up to 20bases),but it only causes 2.6 fold repression according to Johannes H. Urban and Jo?? rg Vogel,for it doesn???t result in the degradation of the RNA complex by RNaseE(ssRNA degradation) and RNaseIII(dsRNA degradation),both of them are major enzymes that causes RNA degradation in vivo.Spot42-galK complex degrades in a slow and presently unclear way. The base pairing is initiated by the recognition of seed region,which plays a significant role in the repression efficiency.
mutable notes: No
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K737031_sequence/1

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxI
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0061
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 579
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
direction: direction/forward
top level: luxI
start: 619
end: 643
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 618
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
direction: direction/forward
top level: luxI
start: 580
end: 611
orientation: inline

BBa_K747025
type: ComponentDefinition
persistent identity: BBa K747025
display id: BBa_K747025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 2
sequence: https://synbiohub.org/public/igem/BBa_K747025_sequence/1

GC 2
type: SequenceAnnotation
persistent identity: annotation2196083
display id: annotation2196083
version: 1
top level: BBa_K747025
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747025/annotation2196083/range2196083/1

https://synbiohub.org/public/igem/BBa_K747025/annotation2196083/range2196083/1
type: Range
persistent identity: range2196083
display id: range2196083
version: 1
direction: direction/forward
top level: BBa_K747025
start: 14
end: 211
orientation: inline

Operon2
type: ComponentDefinition
persistent identity: BBa K808007
display id: BBa_K808007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: putative terephtalate uptake operon2
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12829
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: putative terephtalate uptake operon2 AraProm_RBS_tctA-505_RBS_tctB-197_RBS_tphC
mutable notes: -
mutable provenance: Comamonas testosteroni KF1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K808004
component: BBa_K808005
component: BBa_J61101
component: BBa_J61100
component: BBa_K808001
component: BBa_K808000
sequence annotation: BBa_K808005
sequence annotation: BBa_J61100
sequence annotation: BBa_K808004
sequence annotation: BBa_J61101
sequence annotation: BBa_K808000
sequence annotation: BBa_K808001
sequence: https://synbiohub.org/public/igem/BBa_K808007_sequence/1

BBa_K808004
type: Component
persistent identity: component2201308
display id: component2201308
version: 1
top level: Operon2
access: public
definition: tctA_505aa

BBa_K808005
type: Component
persistent identity: component2201310
display id: component2201310
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_K808005/1

BBa_J61101
type: Component
persistent identity: component2201311
display id: component2201311
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_J61100
type: Component
persistent identity: component2201309
display id: component2201309
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K808001
type: Component
persistent identity: component2201312
display id: component2201312
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_K808001/1

BBa_J61100
type: Component
persistent identity: component2201307
display id: component2201307
version: 1
top level: Operon2
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K808000
type: Component
persistent identity: component2201306
display id: component2201306
version: 1
top level: Operon2
access: public
definition: Ara

BBa_K808005
type: SequenceAnnotation
persistent identity: annotation2201310
display id: annotation2201310
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201310/range2201310/1
component: BBa_K808005

https://synbiohub.org/public/igem/BBa_K808007/annotation2201310/range2201310/1
type: Range
persistent identity: range2201310
display id: range2201310
version: 1
direction: direction/reverse
top level: Operon2
start: 2780
end: 3373
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201309
display id: annotation2201309
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201309/range2201309/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808007/annotation2201309/range2201309/1
type: Range
persistent identity: range2201309
display id: range2201309
version: 1
direction: direction/reverse
top level: Operon2
start: 2762
end: 2773
orientation: inline

BBa_K808004
type: SequenceAnnotation
persistent identity: annotation2201308
display id: annotation2201308
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201308/range2201308/1
component: BBa_K808004

https://synbiohub.org/public/igem/BBa_K808007/annotation2201308/range2201308/1
type: Range
persistent identity: range2201308
display id: range2201308
version: 1
direction: direction/reverse
top level: Operon2
start: 1236
end: 2753
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2201311
display id: annotation2201311
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201311/range2201311/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K808007/annotation2201311/range2201311/1
type: Range
persistent identity: range2201311
display id: range2201311
version: 1
direction: direction/reverse
top level: Operon2
start: 3382
end: 3393
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2201306
display id: annotation2201306
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201306/range2201306/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K808007/annotation2201306/range2201306/1
type: Range
persistent identity: range2201306
display id: range2201306
version: 1
direction: direction/reverse
top level: Operon2
start: 1
end: 1209
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201307
display id: annotation2201307
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201307/range2201307/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808007/annotation2201307/range2201307/1
type: Range
persistent identity: range2201307
display id: range2201307
version: 1
direction: direction/reverse
top level: Operon2
start: 1218
end: 1229
orientation: inline

BBa_K808001
type: SequenceAnnotation
persistent identity: annotation2201312
display id: annotation2201312
version: 1
top level: Operon2
location: https://synbiohub.org/public/igem/BBa_K808007/annotation2201312/range2201312/1
component: BBa_K808001

https://synbiohub.org/public/igem/BBa_K808007/annotation2201312/range2201312/1
type: Range
persistent identity: range2201312
display id: range2201312
version: 1
direction: direction/reverse
top level: Operon2
start: 3400
end: 4368
orientation: inline

BBa_I13519
type: ComponentDefinition
persistent identity: BBa I13519
display id: BBa_I13519
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid Intermediate
creator: jkm
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13452
component: BBa_B0010
component: BBa_B0032
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_I13452
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13519_sequence/1

BBa_I13452
type: Component
persistent identity: component1505565
display id: component1505565
version: 1
top level: BBa_I13519
access: public
definition: https://synbiohub.org/public/igem/BBa_I13452/1

BBa_B0010
type: Component
persistent identity: component1505581
display id: component1505581
version: 1
top level: BBa_I13519
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1505573
display id: component1505573
version: 1
top level: BBa_I13519
access: public
definition: BBa_B0032

BBa_E0040
type: Component
persistent identity: component1505576
display id: component1505576
version: 1
top level: BBa_I13519
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1505591
display id: component1505591
version: 1
top level: BBa_I13519
access: public
definition: BBa_B0012

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1505573
display id: annotation1505573
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505573/range1505573/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13519/annotation1505573/range1505573/1
type: Range
persistent identity: range1505573
display id: range1505573
version: 1
direction: direction/forward
top level: BBa_I13519
start: 61
end: 73
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505591
display id: annotation1505591
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505591/range1505591/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13519/annotation1505591/range1505591/1
type: Range
persistent identity: range1505591
display id: range1505591
version: 1
direction: direction/forward
top level: BBa_I13519
start: 896
end: 936
orientation: inline

BBa_I13452
type: SequenceAnnotation
persistent identity: annotation1505565
display id: annotation1505565
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505565/range1505565/1
component: BBa_I13452

https://synbiohub.org/public/igem/BBa_I13519/annotation1505565/range1505565/1
type: Range
persistent identity: range1505565
display id: range1505565
version: 1
direction: direction/forward
top level: BBa_I13519
start: 1
end: 52
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505576
display id: annotation1505576
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505576/range1505576/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13519/annotation1505576/range1505576/1
type: Range
persistent identity: range1505576
display id: range1505576
version: 1
direction: direction/forward
top level: BBa_I13519
start: 80
end: 799
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505581
display id: annotation1505581
version: 1
top level: BBa_I13519
location: https://synbiohub.org/public/igem/BBa_I13519/annotation1505581/range1505581/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13519/annotation1505581/range1505581/1
type: Range
persistent identity: range1505581
display id: range1505581
version: 1
direction: direction/forward
top level: BBa_I13519
start: 808
end: 887
orientation: inline

BBa_K863021
type: ComponentDefinition
persistent identity: BBa K863021
display id: BBa_K863021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans
creator: Isabel Huber
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CDS
sequence annotation: ATG
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863021_sequence/1

CDS
type: SequenceAnnotation
persistent identity: annotation2213229
display id: annotation2213229
version: 1
top level: BBa_K863021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1
type: Range
persistent identity: range2213229
display id: range2213229
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 1503
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2213230
display id: annotation2213230
version: 1
top level: BBa_K863021
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1
type: Range
persistent identity: range2213230
display id: range2213230
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2213231
display id: annotation2213231
version: 1
top level: BBa_K863021
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1
type: Range
persistent identity: range2213231
display id: range2213231
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1501
end: 1503
orientation: inline

BBa_K786002
type: ComponentDefinition
persistent identity: BBa K786002
display id: BBa_K786002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: HindIII for N-terminal fusin
sequence annotation: BamHI for C-terminal fusion
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: B0015
sequence annotation: SRII-HtrII-Tar
sequence annotation: RBS 1.0
sequence: https://synbiohub.org/public/igem/BBa_K786002_sequence/1

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199691
display id: annotation2199691
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1
type: Range
persistent identity: range2199691
display id: range2199691
version: 1
direction: direction/forward
top level: BBa_K786002
start: 62
end: 67
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199693
display id: annotation2199693
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1
type: Range
persistent identity: range2199693
display id: range2199693
version: 1
direction: direction/forward
top level: BBa_K786002
start: 785
end: 790
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199689
display id: annotation2199689
version: 1
top level: BBa_K786002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1
type: Range
persistent identity: range2199689
display id: range2199689
version: 1
direction: direction/forward
top level: BBa_K786002
start: 1
end: 41
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199694
display id: annotation2199694
version: 1
top level: BBa_K786002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1
type: Range
persistent identity: range2199694
display id: range2199694
version: 1
direction: direction/forward
top level: BBa_K786002
start: 2117
end: 2245
orientation: inline

SRII-HtrII-Tar
type: SequenceAnnotation
persistent identity: annotation2199692
display id: annotation2199692
version: 1
top level: BBa_K786002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1
type: Range
persistent identity: range2199692
display id: range2199692
version: 1
direction: direction/forward
top level: BBa_K786002
start: 68
end: 2083
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199690
display id: annotation2199690
version: 1
top level: BBa_K786002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1
type: Range
persistent identity: range2199690
display id: range2199690
version: 1
direction: direction/forward
top level: BBa_K786002
start: 42
end: 61
orientation: inline

LacIM
type: ComponentDefinition
persistent identity: BBa K819012
display id: BBa_K819012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible LacIM generator
creator: ZHANG Hong
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI mutant driven by luminesensor repressible SulA promoter in 408 form.
mutable notes: B0034, CDS of LacI mutant and B0015 were connected directly to SulA promoter in 408 form.
mutable provenance: Sequene of the LacI mutant is from Partregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: LacIM(K395400)
sequence annotation: SulA408 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K819012_sequence/1

LacIM(K395400)
type: SequenceAnnotation
persistent identity: annotation2181627
display id: annotation2181627
version: 1
top level: LacIM
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819012/annotation2181627/range2181627/1

https://synbiohub.org/public/igem/BBa_K819012/annotation2181627/range2181627/1
type: Range
persistent identity: range2181627
display id: range2181627
version: 1
direction: direction/forward
top level: LacIM
start: 65
end: 1292
orientation: inline

SulA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2181628
display id: annotation2181628
version: 1
top level: LacIM
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819012/annotation2181628/range2181628/1

https://synbiohub.org/public/igem/BBa_K819012/annotation2181628/range2181628/1
type: Range
persistent identity: range2181628
display id: range2181628
version: 1
direction: direction/forward
top level: LacIM
start: 1
end: 64
orientation: inline

BBa_K747064
type: ComponentDefinition
persistent identity: BBa K747064
display id: BBa_K747064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 5
sequence: https://synbiohub.org/public/igem/BBa_K747064_sequence/1

AA 5
type: SequenceAnnotation
persistent identity: annotation2197021
display id: annotation2197021
version: 1
top level: BBa_K747064
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747064/annotation2197021/range2197021/1

https://synbiohub.org/public/igem/BBa_K747064/annotation2197021/range2197021/1
type: Range
persistent identity: range2197021
display id: range2197021
version: 1
direction: direction/forward
top level: BBa_K747064
start: 12
end: 211
orientation: inline

BBa_K747044
type: ComponentDefinition
persistent identity: BBa K747044
display id: BBa_K747044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 3
sequence: https://synbiohub.org/public/igem/BBa_K747044_sequence/1

TA 3
type: SequenceAnnotation
persistent identity: annotation2196261
display id: annotation2196261
version: 1
top level: BBa_K747044
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747044/annotation2196261/range2196261/1

https://synbiohub.org/public/igem/BBa_K747044/annotation2196261/range2196261/1
type: Range
persistent identity: range2196261
display id: range2196261
version: 1
direction: direction/forward
top level: BBa_K747044
start: 14
end: 211
orientation: inline

BBa_I6101
type: ComponentDefinition
persistent identity: BBa I6101
display id: BBa_I6101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0071.E0430
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_R0071
component: BBa_B0012
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0071
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I6101_sequence/1

BBa_B0034
type: Component
persistent identity: component949718
display id: component949718
version: 1
top level: BBa_I6101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0071
type: Component
persistent identity: component949713
display id: component949713
version: 1
top level: BBa_I6101
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0012
type: Component
persistent identity: component949735
display id: component949735
version: 1
top level: BBa_I6101
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component949725
display id: component949725
version: 1
top level: BBa_I6101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component949720
display id: component949720
version: 1
top level: BBa_I6101
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949735
display id: annotation949735
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949735/range949735/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6101/annotation949735/range949735/1
type: Range
persistent identity: range949735
display id: range949735
version: 1
direction: direction/forward
top level: BBa_I6101
start: 899
end: 939
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation949713
display id: annotation949713
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949713/range949713/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_I6101/annotation949713/range949713/1
type: Range
persistent identity: range949713
display id: range949713
version: 1
direction: direction/forward
top level: BBa_I6101
start: 1
end: 53
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation949720
display id: annotation949720
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949720/range949720/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6101/annotation949720/range949720/1
type: Range
persistent identity: range949720
display id: range949720
version: 1
direction: direction/forward
top level: BBa_I6101
start: 80
end: 802
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949725
display id: annotation949725
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949725/range949725/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6101/annotation949725/range949725/1
type: Range
persistent identity: range949725
display id: range949725
version: 1
direction: direction/forward
top level: BBa_I6101
start: 811
end: 890
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949718
display id: annotation949718
version: 1
top level: BBa_I6101
location: https://synbiohub.org/public/igem/BBa_I6101/annotation949718/range949718/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6101/annotation949718/range949718/1
type: Range
persistent identity: range949718
display id: range949718
version: 1
direction: direction/forward
top level: BBa_I6101
start: 62
end: 73
orientation: inline

BBa_I732902
type: ComponentDefinition
persistent identity: BBa I732902
display id: BBa_I732902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010 I732020
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_R0010
component: BBa_B0010
component: BBa_I732006
component: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732902_sequence/1

BBa_B0012
type: Component
persistent identity: component1948546
display id: component1948546
version: 1
top level: BBa_I732902
access: public
definition: BBa_B0012

BBa_R0010
type: Component
persistent identity: component1948531
display id: component1948531
version: 1
top level: BBa_I732902
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component1948544
display id: component1948544
version: 1
top level: BBa_I732902
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I732006
type: Component
persistent identity: component1948543
display id: component1948543
version: 1
top level: BBa_I732902
access: public
definition: https://synbiohub.org/public/igem/BBa_I732006/1

BBa_B0034
type: Component
persistent identity: component1948539
display id: component1948539
version: 1
top level: BBa_I732902
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948543
display id: annotation1948543
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948543/range1948543/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732902/annotation1948543/range1948543/1
type: Range
persistent identity: range1948543
display id: range1948543
version: 1
direction: direction/reverse
top level: BBa_I732902
start: 227
end: 460
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948546
display id: annotation1948546
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948546/range1948546/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732902/annotation1948546/range1948546/1
type: Range
persistent identity: range1948546
display id: range1948546
version: 1
direction: direction/forward
top level: BBa_I732902
start: 557
end: 597
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1948531
display id: annotation1948531
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948531/range1948531/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I732902/annotation1948531/range1948531/1
type: Range
persistent identity: range1948531
display id: range1948531
version: 1
direction: direction/forward
top level: BBa_I732902
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948544
display id: annotation1948544
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948544/range1948544/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732902/annotation1948544/range1948544/1
type: Range
persistent identity: range1948544
display id: range1948544
version: 1
direction: direction/forward
top level: BBa_I732902
start: 469
end: 548
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948539
display id: annotation1948539
version: 1
top level: BBa_I732902
location: https://synbiohub.org/public/igem/BBa_I732902/annotation1948539/range1948539/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732902/annotation1948539/range1948539/1
type: Range
persistent identity: range1948539
display id: range1948539
version: 1
direction: direction/reverse
top level: BBa_I732902
start: 209
end: 220
orientation: inline

BBa_K797000
type: ComponentDefinition
persistent identity: BBa K797000
display id: BBa_K797000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tatABCD from E.coli
creator: Tomohiro Nobeyama
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
group u list: _1053_
m user id: 0
owner id: 11588
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is involved in TAT protein export pathway of E.coli. The part consist of three genes expressing tatA,B,C proteins. They are the components of Tat complex on cell membrane, where protein with torA signal can go through
mutable notes: This parts have very long region so that we have to divide this parts into two regions to read sequence.
mutable provenance: They are derived from tatABCD operon in Escherichia coli???s genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tatA
sequence annotation: tatD
sequence annotation: tatB
sequence annotation: tatC
sequence: https://synbiohub.org/public/igem/BBa_K797000_sequence/1

tatA
type: SequenceAnnotation
persistent identity: annotation2194797
display id: annotation2194797
version: 1
top level: BBa_K797000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797000/annotation2194797/range2194797/1

https://synbiohub.org/public/igem/BBa_K797000/annotation2194797/range2194797/1
type: Range
persistent identity: range2194797
display id: range2194797
version: 1
direction: direction/forward
top level: BBa_K797000
start: 119
end: 389
orientation: inline

tatD
type: SequenceAnnotation
persistent identity: annotation2197836
display id: annotation2197836
version: 1
top level: BBa_K797000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797000/annotation2197836/range2197836/1

https://synbiohub.org/public/igem/BBa_K797000/annotation2197836/range2197836/1
type: Range
persistent identity: range2197836
display id: range2197836
version: 1
direction: direction/forward
top level: BBa_K797000
start: 1716
end: 2511
orientation: inline

tatB
type: SequenceAnnotation
persistent identity: annotation2197721
display id: annotation2197721
version: 1
top level: BBa_K797000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797000/annotation2197721/range2197721/1

https://synbiohub.org/public/igem/BBa_K797000/annotation2197721/range2197721/1
type: Range
persistent identity: range2197721
display id: range2197721
version: 1
direction: direction/forward
top level: BBa_K797000
start: 393
end: 907
orientation: inline

tatC
type: SequenceAnnotation
persistent identity: annotation2197737
display id: annotation2197737
version: 1
top level: BBa_K797000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797000/annotation2197737/range2197737/1

https://synbiohub.org/public/igem/BBa_K797000/annotation2197737/range2197737/1
type: Range
persistent identity: range2197737
display id: range2197737
version: 1
direction: direction/forward
top level: BBa_K797000
start: 911
end: 1687
orientation: inline

BBa_K747011
type: ComponentDefinition
persistent identity: BBa K747011
display id: BBa_K747011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 1
sequence: https://synbiohub.org/public/igem/BBa_K747011_sequence/1

GT 1
type: SequenceAnnotation
persistent identity: annotation2195848
display id: annotation2195848
version: 1
top level: BBa_K747011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747011/annotation2195848/range2195848/1

https://synbiohub.org/public/igem/BBa_K747011/annotation2195848/range2195848/1
type: Range
persistent identity: range2195848
display id: range2195848
version: 1
direction: direction/forward
top level: BBa_K747011
start: 12
end: 209
orientation: inline

BBa_K876010
type: ComponentDefinition
persistent identity: BBa K876010
display id: BBa_K876010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux-TetR
creator: Timothy Cogan
created: 2012-09-29T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
group u list: _1138_
m user id: 0
owner id: 13836
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inducible promoter for TetR
mutable notes: Biobrick placement
mutable provenance: Generated from biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0040
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K876010_sequence/1

BBa_C0040
type: Component
persistent identity: component2245105
display id: component2245105
version: 1
top level: BBa_K876010
access: public
definition: tetR

BBa_R0062
type: Component
persistent identity: component2245097
display id: component2245097
version: 1
top level: BBa_K876010
access: public
definition: lux pR

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2245097
display id: annotation2245097
version: 1
top level: BBa_K876010
location: https://synbiohub.org/public/igem/BBa_K876010/annotation2245097/range2245097/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K876010/annotation2245097/range2245097/1
type: Range
persistent identity: range2245097
display id: range2245097
version: 1
direction: direction/forward
top level: BBa_K876010
start: 1
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2245105
display id: annotation2245105
version: 1
top level: BBa_K876010
location: https://synbiohub.org/public/igem/BBa_K876010/annotation2245105/range2245105/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K876010/annotation2245105/range2245105/1
type: Range
persistent identity: range2245105
display id: range2245105
version: 1
direction: direction/reverse
top level: BBa_K876010
start: 62
end: 746
orientation: inline

BBa_I6211
type: ComponentDefinition
persistent identity: BBa I6211
display id: BBa_I6211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0083.E0432
creator: Jennifer Braff
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_E0032
component: BBa_B0012
component: BBa_R0083
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0083
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I6211_sequence/1

BBa_B0010
type: Component
persistent identity: component950789
display id: component950789
version: 1
top level: BBa_I6211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component950769
display id: component950769
version: 1
top level: BBa_I6211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component950781
display id: component950781
version: 1
top level: BBa_I6211
access: public
definition: YFP

BBa_B0012
type: Component
persistent identity: component950799
display id: component950799
version: 1
top level: BBa_I6211
access: public
definition: BBa_B0012

BBa_R0083
type: Component
persistent identity: component950764
display id: component950764
version: 1
top level: BBa_I6211
access: public
definition: https://synbiohub.org/public/igem/BBa_R0083/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation950769
display id: annotation950769
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950769/range950769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6211/annotation950769/range950769/1
type: Range
persistent identity: range950769
display id: range950769
version: 1
direction: direction/forward
top level: BBa_I6211
start: 87
end: 98
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation950799
display id: annotation950799
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950799/range950799/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6211/annotation950799/range950799/1
type: Range
persistent identity: range950799
display id: range950799
version: 1
direction: direction/forward
top level: BBa_I6211
start: 963
end: 1003
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation950789
display id: annotation950789
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950789/range950789/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6211/annotation950789/range950789/1
type: Range
persistent identity: range950789
display id: range950789
version: 1
direction: direction/forward
top level: BBa_I6211
start: 875
end: 954
orientation: inline

BBa_R0083
type: SequenceAnnotation
persistent identity: annotation950764
display id: annotation950764
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950764/range950764/1
component: BBa_R0083

https://synbiohub.org/public/igem/BBa_I6211/annotation950764/range950764/1
type: Range
persistent identity: range950764
display id: range950764
version: 1
direction: direction/forward
top level: BBa_I6211
start: 1
end: 78
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation950781
display id: annotation950781
version: 1
top level: BBa_I6211
location: https://synbiohub.org/public/igem/BBa_I6211/annotation950781/range950781/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6211/annotation950781/range950781/1
type: Range
persistent identity: range950781
display id: range950781
version: 1
direction: direction/forward
top level: BBa_I6211
start: 105
end: 866
orientation: inline

BBa_K863006
type: ComponentDefinition
persistent identity: BBa K863006
display id: BBa_K863006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: E.coli laccase ORF
mutable notes: test
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863006_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184436
display id: annotation2184436
version: 1
top level: BBa_K863006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1
type: Range
persistent identity: range2184436
display id: range2184436
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1549
end: 1551
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184435
display id: annotation2184435
version: 1
top level: BBa_K863006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1
type: Range
persistent identity: range2184435
display id: range2184435
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1
end: 3
orientation: inline

BBa_K747082
type: ComponentDefinition
persistent identity: BBa K747082
display id: BBa_K747082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 6
sequence: https://synbiohub.org/public/igem/BBa_K747082_sequence/1

AC 6
type: SequenceAnnotation
persistent identity: annotation2197055
display id: annotation2197055
version: 1
top level: BBa_K747082
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747082/annotation2197055/range2197055/1

https://synbiohub.org/public/igem/BBa_K747082/annotation2197055/range2197055/1
type: Range
persistent identity: range2197055
display id: range2197055
version: 1
direction: direction/forward
top level: BBa_K747082
start: 14
end: 211
orientation: inline

BBa_K775000
type: ComponentDefinition
persistent identity: BBa K775000
display id: BBa_K775000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-GPR109A Niacin chimeric GPCR
creator: Sietske Grijseels
created: 2012-08-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: Promoter: GPD Terminator: CYC1.
mutable provenance: Homo sapiens NP_808219.1 Rat
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: N terminus RI7
sequence annotation: Kozak sequence
sequence annotation: Stopcodon
sequence annotation: NdeI restriction site
sequence annotation: BamHI restriction site
sequence annotation: CYC1 terminator
sequence annotation: Startcodon
sequence annotation: FLAGtag
sequence annotation: Niacin receptor part
sequence annotation: GPD promoter
sequence annotation: C terminus RI7
sequence: https://synbiohub.org/public/igem/BBa_K775000_sequence/1

N terminus RI7
type: SequenceAnnotation
persistent identity: annotation2181643
display id: annotation2181643
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181643/range2181643/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181643/range2181643/1
type: Range
persistent identity: range2181643
display id: range2181643
version: 1
direction: direction/forward
top level: BBa_K775000
start: 152
end: 337
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2181640
display id: annotation2181640
version: 1
top level: BBa_K775000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181640/range2181640/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181640/range2181640/1
type: Range
persistent identity: range2181640
display id: range2181640
version: 1
direction: direction/forward
top level: BBa_K775000
start: 119
end: 124
orientation: inline

Stopcodon
type: SequenceAnnotation
persistent identity: annotation2181644
display id: annotation2181644
version: 1
top level: BBa_K775000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181644/range2181644/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181644/range2181644/1
type: Range
persistent identity: range2181644
display id: range2181644
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1151
end: 1156
orientation: inline

NdeI restriction site
type: SequenceAnnotation
persistent identity: annotation2181645
display id: annotation2181645
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181645/range2181645/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181645/range2181645/1
type: Range
persistent identity: range2181645
display id: range2181645
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1157
end: 1162
orientation: inline

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2181639
display id: annotation2181639
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181639/range2181639/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181639/range2181639/1
type: Range
persistent identity: range2181639
display id: range2181639
version: 1
direction: direction/forward
top level: BBa_K775000
start: 113
end: 118
orientation: inline

CYC1 terminator
type: SequenceAnnotation
persistent identity: annotation2181646
display id: annotation2181646
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181646/range2181646/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181646/range2181646/1
type: Range
persistent identity: range2181646
display id: range2181646
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1163
end: 1414
orientation: inline

Startcodon
type: SequenceAnnotation
persistent identity: annotation2181641
display id: annotation2181641
version: 1
top level: BBa_K775000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181641/range2181641/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181641/range2181641/1
type: Range
persistent identity: range2181641
display id: range2181641
version: 1
direction: direction/forward
top level: BBa_K775000
start: 125
end: 127
orientation: inline

FLAGtag
type: SequenceAnnotation
persistent identity: annotation2181642
display id: annotation2181642
version: 1
top level: BBa_K775000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181642/range2181642/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181642/range2181642/1
type: Range
persistent identity: range2181642
display id: range2181642
version: 1
direction: direction/forward
top level: BBa_K775000
start: 128
end: 151
orientation: inline

Niacin receptor part
type: SequenceAnnotation
persistent identity: annotation2181648
display id: annotation2181648
version: 1
top level: BBa_K775000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181648/range2181648/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181648/range2181648/1
type: Range
persistent identity: range2181648
display id: range2181648
version: 1
direction: direction/forward
top level: BBa_K775000
start: 338
end: 1054
orientation: inline

GPD promoter
type: SequenceAnnotation
persistent identity: annotation2181638
display id: annotation2181638
version: 1
top level: BBa_K775000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181638/range2181638/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181638/range2181638/1
type: Range
persistent identity: range2181638
display id: range2181638
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1
end: 112
orientation: inline

C terminus RI7
type: SequenceAnnotation
persistent identity: annotation2181647
display id: annotation2181647
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181647/range2181647/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181647/range2181647/1
type: Range
persistent identity: range2181647
display id: range2181647
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1055
end: 1150
orientation: inline

BBa_I6050
type: ComponentDefinition
persistent identity: BBa I6050
display id: BBa_I6050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R1051.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_E0030
component: BBa_B0010
component: BBa_R1051
component: BBa_B0012
sequence annotation: BBa_R1051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I6050_sequence/1

BBa_B0034
type: Component
persistent identity: component948692
display id: component948692
version: 1
top level: BBa_I6050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component948694
display id: component948694
version: 1
top level: BBa_I6050
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component948699
display id: component948699
version: 1
top level: BBa_I6050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R1051
type: Component
persistent identity: component948684
display id: component948684
version: 1
top level: BBa_I6050
access: public
definition: https://synbiohub.org/public/igem/BBa_R1051/1

BBa_B0012
type: Component
persistent identity: component948709
display id: component948709
version: 1
top level: BBa_I6050
access: public
definition: BBa_B0012

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation948684
display id: annotation948684
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948684/range948684/1
component: BBa_R1051

https://synbiohub.org/public/igem/BBa_I6050/annotation948684/range948684/1
type: Range
persistent identity: range948684
display id: range948684
version: 1
direction: direction/forward
top level: BBa_I6050
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948692
display id: annotation948692
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948692/range948692/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6050/annotation948692/range948692/1
type: Range
persistent identity: range948692
display id: range948692
version: 1
direction: direction/forward
top level: BBa_I6050
start: 58
end: 69
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948699
display id: annotation948699
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948699/range948699/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6050/annotation948699/range948699/1
type: Range
persistent identity: range948699
display id: range948699
version: 1
direction: direction/forward
top level: BBa_I6050
start: 807
end: 886
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948709
display id: annotation948709
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948709/range948709/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6050/annotation948709/range948709/1
type: Range
persistent identity: range948709
display id: range948709
version: 1
direction: direction/forward
top level: BBa_I6050
start: 895
end: 935
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948694
display id: annotation948694
version: 1
top level: BBa_I6050
location: https://synbiohub.org/public/igem/BBa_I6050/annotation948694/range948694/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6050/annotation948694/range948694/1
type: Range
persistent identity: range948694
display id: range948694
version: 1
direction: direction/forward
top level: BBa_I6050
start: 76
end: 798
orientation: inline

BBa_K748003
type: ComponentDefinition
persistent identity: BBa K748003
display id: BBa_K748003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yddV is a Diguanylate Cyclase-Genomic.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
mutable notes: nothing special
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K748003_sequence/1

BBa_K737005
type: ComponentDefinition
persistent identity: BBa K737005
display id: BBa_K737005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible GVPC Generator
creator: Li Kang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains a constitutive promoter (J23106), a coding region of TetR protein, a terminator (B0015), a promoter repressed by TetR protein (R0040) a coding region of GvpC and GFP and a terminator (B0015) behind it. This part can be used to induce the strength of promoter (R0040) by Tet and indicate the content of GvpC by fluorescence of GFP. In short, it can regulate the expression of GvpC. We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP. Here is the curve:
mutable notes: We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K737017
component: BBa_E0840
component: BBa_P0440
component: BBa_R0040
component: BBa_B0034
component: BBa_J23106
sequence annotation: BBa_P0440
sequence annotation: BBa_E0840
sequence annotation: BBa_K737017
sequence annotation: BBa_B0034
sequence annotation: BBa_J23106
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K737005_sequence/1

BBa_K737017
type: Component
persistent identity: component2184861
display id: component2184861
version: 1
top level: BBa_K737005
access: public
definition: https://synbiohub.org/public/igem/BBa_K737017/1

BBa_E0840
type: Component
persistent identity: component2184872
display id: component2184872
version: 1
top level: BBa_K737005
access: public
definition: GFP genera

BBa_P0440
type: Component
persistent identity: component2184853
display id: component2184853
version: 1
top level: BBa_K737005
access: public
definition: BBa_P0440

BBa_R0040
type: Component
persistent identity: component2184854
display id: component2184854
version: 1
top level: BBa_K737005
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component2184860
display id: component2184860
version: 1
top level: BBa_K737005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23106
type: Component
persistent identity: component2184840
display id: component2184840
version: 1
top level: BBa_K737005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_P0440
type: SequenceAnnotation
persistent identity: annotation2184853
display id: annotation2184853
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184853/range2184853/1
component: BBa_P0440

https://synbiohub.org/public/igem/BBa_K737005/annotation2184853/range2184853/1
type: Range
persistent identity: range2184853
display id: range2184853
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 44
end: 883
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2184872
display id: annotation2184872
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184872/range2184872/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K737005/annotation2184872/range2184872/1
type: Range
persistent identity: range2184872
display id: range2184872
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 1484
end: 2361
orientation: inline

BBa_K737017
type: SequenceAnnotation
persistent identity: annotation2184861
display id: annotation2184861
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184861/range2184861/1
component: BBa_K737017

https://synbiohub.org/public/igem/BBa_K737005/annotation2184861/range2184861/1
type: Range
persistent identity: range2184861
display id: range2184861
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 972
end: 1475
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2184860
display id: annotation2184860
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184860/range2184860/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K737005/annotation2184860/range2184860/1
type: Range
persistent identity: range2184860
display id: range2184860
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 954
end: 965
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2184840
display id: annotation2184840
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184840/range2184840/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K737005/annotation2184840/range2184840/1
type: Range
persistent identity: range2184840
display id: range2184840
version: 1
direction: direction/reverse
top level: BBa_K737005
start: 1
end: 35
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2184854
display id: annotation2184854
version: 1
top level: BBa_K737005
location: https://synbiohub.org/public/igem/BBa_K737005/annotation2184854/range2184854/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K737005/annotation2184854/range2184854/1
type: Range
persistent identity: range2184854
display id: range2184854
version: 1
direction: direction/forward
top level: BBa_K737005
start: 892
end: 945
orientation: inline

BBa_K863005
type: ComponentDefinition
persistent identity: BBa K863005
display id: BBa_K863005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: E.coli laccase ORF with T7, RBS and HIS tag
mutable notes: TEST
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: T7 promoter
sequence annotation: ecol
sequence annotation: 6x HIS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K863005_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184563
display id: annotation2184563
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1
type: Range
persistent identity: range2184563
display id: range2184563
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1603
end: 1605
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184564
display id: annotation2184564
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1
type: Range
persistent identity: range2184564
display id: range2184564
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1600
end: 1602
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184562
display id: annotation2184562
version: 1
top level: BBa_K863005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1
type: Range
persistent identity: range2184562
display id: range2184562
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 36
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184561
display id: annotation2184561
version: 1
top level: BBa_K863005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1
type: Range
persistent identity: range2184561
display id: range2184561
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1
end: 23
orientation: inline

ecol
type: SequenceAnnotation
persistent identity: annotation2184566
display id: annotation2184566
version: 1
top level: BBa_K863005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1
type: Range
persistent identity: range2184566
display id: range2184566
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 1581
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184565
display id: annotation2184565
version: 1
top level: BBa_K863005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1
type: Range
persistent identity: range2184565
display id: range2184565
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1582
end: 1599
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187301
display id: annotation2187301
version: 1
top level: BBa_K863005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1
type: Range
persistent identity: range2187301
display id: range2187301
version: 1
direction: direction/forward
top level: BBa_K863005
start: 25
end: 30
orientation: inline

BBa_K747072
type: ComponentDefinition
persistent identity: BBa K747072
display id: BBa_K747072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 5
sequence: https://synbiohub.org/public/igem/BBa_K747072_sequence/1

GA 5
type: SequenceAnnotation
persistent identity: annotation2197039
display id: annotation2197039
version: 1
top level: BBa_K747072
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747072/annotation2197039/range2197039/1

https://synbiohub.org/public/igem/BBa_K747072/annotation2197039/range2197039/1
type: Range
persistent identity: range2197039
display id: range2197039
version: 1
direction: direction/forward
top level: BBa_K747072
start: 14
end: 211
orientation: inline

BBa_K909015
type: ComponentDefinition
persistent identity: BBa K909015
display id: BBa_K909015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p-ABA overproduction composite without promoter
creator: Deborah Huber
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1174_
m user id: 0
owner id: 14088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of pABA. ADC synthase is composed of two subunits, PabA and PabB. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates PABA.
mutable notes: pabB and pabA constitute an operon that can be controled by any promoter.
mutable provenance: S04039, K137055
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K137006
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_K137005
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K137006
sequence annotation: BBa_K137005
sequence: https://synbiohub.org/public/igem/BBa_K909015_sequence/1

BBa_K137006
type: Component
persistent identity: component2193607
display id: component2193607
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_K137006/1

BBa_B0034
type: Component
persistent identity: component2193606
display id: component2193606
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2193609
display id: component2193609
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2193611
display id: component2193611
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2193613
display id: component2193613
version: 1
top level: BBa_K909015
access: public
definition: BBa_B0012

BBa_K137005
type: Component
persistent identity: component2193610
display id: component2193610
version: 1
top level: BBa_K909015
access: public
definition: https://synbiohub.org/public/igem/BBa_K137005/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2193606
display id: annotation2193606
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193606/range2193606/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909015/annotation2193606/range2193606/1
type: Range
persistent identity: range2193606
display id: range2193606
version: 1
direction: direction/reverse
top level: BBa_K909015
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2193611
display id: annotation2193611
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193611/range2193611/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K909015/annotation2193611/range2193611/1
type: Range
persistent identity: range2193611
display id: range2193611
version: 1
direction: direction/forward
top level: BBa_K909015
start: 2528
end: 2607
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2193613
display id: annotation2193613
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193613/range2193613/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K909015/annotation2193613/range2193613/1
type: Range
persistent identity: range2193613
display id: range2193613
version: 1
direction: direction/forward
top level: BBa_K909015
start: 2616
end: 2656
orientation: inline

BBa_K137006
type: SequenceAnnotation
persistent identity: annotation2193607
display id: annotation2193607
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193607/range2193607/1
component: BBa_K137006

https://synbiohub.org/public/igem/BBa_K909015/annotation2193607/range2193607/1
type: Range
persistent identity: range2193607
display id: range2193607
version: 1
direction: direction/reverse
top level: BBa_K909015
start: 19
end: 1908
orientation: inline

BBa_K137005
type: SequenceAnnotation
persistent identity: annotation2193610
display id: annotation2193610
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193610/range2193610/1
component: BBa_K137005

https://synbiohub.org/public/igem/BBa_K909015/annotation2193610/range2193610/1
type: Range
persistent identity: range2193610
display id: range2193610
version: 1
direction: direction/reverse
top level: BBa_K909015
start: 1935
end: 2519
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2193609
display id: annotation2193609
version: 1
top level: BBa_K909015
location: https://synbiohub.org/public/igem/BBa_K909015/annotation2193609/range2193609/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909015/annotation2193609/range2193609/1
type: Range
persistent identity: range2193609
display id: range2193609
version: 1
direction: direction/reverse
top level: BBa_K909015
start: 1917
end: 1928
orientation: inline

BBa_K747016
type: ComponentDefinition
persistent identity: BBa K747016
display id: BBa_K747016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 2
sequence: https://synbiohub.org/public/igem/BBa_K747016_sequence/1

AA 2
type: SequenceAnnotation
persistent identity: annotation2195965
display id: annotation2195965
version: 1
top level: BBa_K747016
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747016/annotation2195965/range2195965/1

https://synbiohub.org/public/igem/BBa_K747016/annotation2195965/range2195965/1
type: Range
persistent identity: range2195965
display id: range2195965
version: 1
direction: direction/forward
top level: BBa_K747016
start: 14
end: 211
orientation: inline

BBa_K741002
type: ComponentDefinition
persistent identity: BBa K741002
display id: BBa_K741002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac-RBS-GFP-T
creator: Wuyang Chen
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Different strength of green fluorescence will be generated determined by the concentration of IPTG.
mutable notes: TBD
mutable provenance: BBa_R0010 BBa_E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13504
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K741002_sequence/1

BBa_I13504
type: Component
persistent identity: component2177894
display id: component2177894
version: 1
top level: BBa_K741002
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_R0010
type: Component
persistent identity: component2177878
display id: component2177878
version: 1
top level: BBa_K741002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2177878
display id: annotation2177878
version: 1
top level: BBa_K741002
location: https://synbiohub.org/public/igem/BBa_K741002/annotation2177878/range2177878/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K741002/annotation2177878/range2177878/1
type: Range
persistent identity: range2177878
display id: range2177878
version: 1
direction: direction/forward
top level: BBa_K741002
start: 1
end: 200
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2177894
display id: annotation2177894
version: 1
top level: BBa_K741002
location: https://synbiohub.org/public/igem/BBa_K741002/annotation2177894/range2177894/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K741002/annotation2177894/range2177894/1
type: Range
persistent identity: range2177894
display id: range2177894
version: 1
direction: direction/reverse
top level: BBa_K741002
start: 209
end: 1083
orientation: inline

BBa_K747039
type: ComponentDefinition
persistent identity: BBa K747039
display id: BBa_K747039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 3
sequence: https://synbiohub.org/public/igem/BBa_K747039_sequence/1

CT 3
type: SequenceAnnotation
persistent identity: annotation2196224
display id: annotation2196224
version: 1
top level: BBa_K747039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747039/annotation2196224/range2196224/1

https://synbiohub.org/public/igem/BBa_K747039/annotation2196224/range2196224/1
type: Range
persistent identity: range2196224
display id: range2196224
version: 1
direction: direction/forward
top level: BBa_K747039
start: 14
end: 211
orientation: inline

BBa_I6042
type: ComponentDefinition
persistent identity: BBa I6042
display id: BBa_I6042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0062.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0062
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6042_sequence/1

BBa_B0034
type: Component
persistent identity: component948542
display id: component948542
version: 1
top level: BBa_I6042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component948559
display id: component948559
version: 1
top level: BBa_I6042
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component948549
display id: component948549
version: 1
top level: BBa_I6042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component948534
display id: component948534
version: 1
top level: BBa_I6042
access: public
definition: lux pR

BBa_E0030
type: Component
persistent identity: component948544
display id: component948544
version: 1
top level: BBa_I6042
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948542
display id: annotation948542
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948542/range948542/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6042/annotation948542/range948542/1
type: Range
persistent identity: range948542
display id: range948542
version: 1
direction: direction/forward
top level: BBa_I6042
start: 64
end: 75
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948544
display id: annotation948544
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948544/range948544/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6042/annotation948544/range948544/1
type: Range
persistent identity: range948544
display id: range948544
version: 1
direction: direction/forward
top level: BBa_I6042
start: 82
end: 804
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948559
display id: annotation948559
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948559/range948559/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6042/annotation948559/range948559/1
type: Range
persistent identity: range948559
display id: range948559
version: 1
direction: direction/forward
top level: BBa_I6042
start: 901
end: 941
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation948534
display id: annotation948534
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948534/range948534/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I6042/annotation948534/range948534/1
type: Range
persistent identity: range948534
display id: range948534
version: 1
direction: direction/forward
top level: BBa_I6042
start: 1
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948549
display id: annotation948549
version: 1
top level: BBa_I6042
location: https://synbiohub.org/public/igem/BBa_I6042/annotation948549/range948549/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6042/annotation948549/range948549/1
type: Range
persistent identity: range948549
display id: range948549
version: 1
direction: direction/forward
top level: BBa_I6042
start: 813
end: 892
orientation: inline

araC-CysEm
type: ComponentDefinition
persistent identity: BBa K731030
display id: BBa_K731030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible araC-pBAD promoter regulating M256I CysE
creator: Jason Fontana, Daniele Rossetto
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose.
mutable notes: K731200 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency. The former was cut with E&S, the latter with X&P. pSB1C3 was digested with E&P.
mutable provenance: K731200, K731010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K731201
component: BBa_K731010
sequence annotation: BBa_K731201
sequence annotation: BBa_K731010
sequence: https://synbiohub.org/public/igem/BBa_K731030_sequence/1

BBa_K731201
type: Component
persistent identity: component2331178
display id: component2331178
version: 1
top level: araC-CysEm
access: public
definition: https://synbiohub.org/public/igem/BBa_K731201/1

BBa_K731010
type: Component
persistent identity: component2331183
display id: component2331183
version: 1
top level: araC-CysEm
access: public
definition: https://synbiohub.org/public/igem/BBa_K731010/1

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2331178
display id: annotation2331178
version: 1
top level: araC-CysEm
location: https://synbiohub.org/public/igem/BBa_K731030/annotation2331178/range2331178/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K731030/annotation2331178/range2331178/1
type: Range
persistent identity: range2331178
display id: range2331178
version: 1
direction: direction/reverse
top level: araC-CysEm
start: 1
end: 1203
orientation: inline

BBa_K731010
type: SequenceAnnotation
persistent identity: annotation2331183
display id: annotation2331183
version: 1
top level: araC-CysEm
location: https://synbiohub.org/public/igem/BBa_K731030/annotation2331183/range2331183/1
component: BBa_K731010

https://synbiohub.org/public/igem/BBa_K731030/annotation2331183/range2331183/1
type: Range
persistent identity: range2331183
display id: range2331183
version: 1
direction: direction/reverse
top level: araC-CysEm
start: 1212
end: 2050
orientation: inline

BBa_K909014
type: ComponentDefinition
persistent identity: BBa K909014
display id: BBa_K909014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive expression of pABA
creator: Deborah Huber
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of pABA. ADC synthase is composed of two subunits, PabA and PabB. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates PABA.
mutable notes: xyz
mutable provenance: Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli. V K Viswanathan,; J M Green and; B P Nichols.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K137006
component: BBa_B0034
component: BBa_B0010
component: BBa_J23100
component: BBa_B0012
component: BBa_K137005
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K137006
sequence annotation: BBa_K137005
sequence annotation: BBa_J23100
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K909014_sequence/1

BBa_K137006
type: Component
persistent identity: component2193593
display id: component2193593
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_K137006/1

BBa_B0034
type: Component
persistent identity: component2193592
display id: component2193592
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2193595
display id: component2193595
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2193597
display id: component2193597
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23100
type: Component
persistent identity: component2193590
display id: component2193590
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0012
type: Component
persistent identity: component2193599
display id: component2193599
version: 1
top level: BBa_K909014
access: public
definition: BBa_B0012

BBa_K137005
type: Component
persistent identity: component2193596
display id: component2193596
version: 1
top level: BBa_K909014
access: public
definition: https://synbiohub.org/public/igem/BBa_K137005/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2193595
display id: annotation2193595
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193595/range2193595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909014/annotation2193595/range2193595/1
type: Range
persistent identity: range2193595
display id: range2193595
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 1960
end: 1971
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2193599
display id: annotation2193599
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193599/range2193599/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K909014/annotation2193599/range2193599/1
type: Range
persistent identity: range2193599
display id: range2193599
version: 1
direction: direction/forward
top level: BBa_K909014
start: 2659
end: 2699
orientation: inline

BBa_K137006
type: SequenceAnnotation
persistent identity: annotation2193593
display id: annotation2193593
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193593/range2193593/1
component: BBa_K137006

https://synbiohub.org/public/igem/BBa_K909014/annotation2193593/range2193593/1
type: Range
persistent identity: range2193593
display id: range2193593
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 62
end: 1951
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2193592
display id: annotation2193592
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193592/range2193592/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909014/annotation2193592/range2193592/1
type: Range
persistent identity: range2193592
display id: range2193592
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 44
end: 55
orientation: inline

BBa_K137005
type: SequenceAnnotation
persistent identity: annotation2193596
display id: annotation2193596
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193596/range2193596/1
component: BBa_K137005

https://synbiohub.org/public/igem/BBa_K909014/annotation2193596/range2193596/1
type: Range
persistent identity: range2193596
display id: range2193596
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 1978
end: 2562
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2193590
display id: annotation2193590
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193590/range2193590/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K909014/annotation2193590/range2193590/1
type: Range
persistent identity: range2193590
display id: range2193590
version: 1
direction: direction/reverse
top level: BBa_K909014
start: 1
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2193597
display id: annotation2193597
version: 1
top level: BBa_K909014
location: https://synbiohub.org/public/igem/BBa_K909014/annotation2193597/range2193597/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K909014/annotation2193597/range2193597/1
type: Range
persistent identity: range2193597
display id: range2193597
version: 1
direction: direction/forward
top level: BBa_K909014
start: 2571
end: 2650
orientation: inline

BBa_K747043
type: ComponentDefinition
persistent identity: BBa K747043
display id: BBa_K747043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 3
sequence: https://synbiohub.org/public/igem/BBa_K747043_sequence/1

GT 3
type: SequenceAnnotation
persistent identity: annotation2196260
display id: annotation2196260
version: 1
top level: BBa_K747043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747043/annotation2196260/range2196260/1

https://synbiohub.org/public/igem/BBa_K747043/annotation2196260/range2196260/1
type: Range
persistent identity: range2196260
display id: range2196260
version: 1
direction: direction/forward
top level: BBa_K747043
start: 14
end: 211
orientation: inline

BBa_K769022
type: ComponentDefinition
persistent identity: BBa K769022
display id: BBa_K769022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lux operon(K769011) + GFP(I13522)
creator: Tomomi YOKOYAMA
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:14Z
discontinued: false
dominant: false
group u list: _1021_
m user id: 0
owner id: 14294
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lux operon(K769011) + GFP(I13522)
mutable notes: amprify green light
mutable provenance: P.kishitanii
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K769011
component: BBa_I13522
sequence annotation: BBa_I13522
sequence annotation: BBa_K769011
sequence: https://synbiohub.org/public/igem/BBa_K769022_sequence/1

BBa_K769011
type: Component
persistent identity: component2186513
display id: component2186513
version: 1
top level: BBa_K769022
access: public
definition: https://synbiohub.org/public/igem/BBa_K769011/1

BBa_I13522
type: Component
persistent identity: component2186528
display id: component2186528
version: 1
top level: BBa_K769022
access: public
definition: https://synbiohub.org/public/igem/BBa_I13522/1

BBa_I13522
type: SequenceAnnotation
persistent identity: annotation2186528
display id: annotation2186528
version: 1
top level: BBa_K769022
location: https://synbiohub.org/public/igem/BBa_K769022/annotation2186528/range2186528/1
component: BBa_I13522

https://synbiohub.org/public/igem/BBa_K769022/annotation2186528/range2186528/1
type: Range
persistent identity: range2186528
display id: range2186528
version: 1
direction: direction/reverse
top level: BBa_K769022
start: 8540
end: 9476
orientation: inline

BBa_K769011
type: SequenceAnnotation
persistent identity: annotation2186513
display id: annotation2186513
version: 1
top level: BBa_K769022
location: https://synbiohub.org/public/igem/BBa_K769022/annotation2186513/range2186513/1
component: BBa_K769011

https://synbiohub.org/public/igem/BBa_K769022/annotation2186513/range2186513/1
type: Range
persistent identity: range2186513
display id: range2186513
version: 1
direction: direction/reverse
top level: BBa_K769022
start: 1
end: 8531
orientation: inline

BBa_J202008
type: ComponentDefinition
persistent identity: BBa J202008
display id: BBa_J202008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#9
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -60 relative to the araC transcriptional start site was changed from C to T.
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI1
sequence annotation: Transcription Start
sequence annotation: Mutation
sequence annotation: araI2
sequence: https://synbiohub.org/public/igem/BBa_J202008_sequence/1

araI1
type: SequenceAnnotation
persistent identity: annotation2178449
display id: annotation2178449
version: 1
top level: BBa_J202008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202008/annotation2178449/range2178449/1

https://synbiohub.org/public/igem/BBa_J202008/annotation2178449/range2178449/1
type: Range
persistent identity: range2178449
display id: range2178449
version: 1
direction: direction/forward
top level: BBa_J202008
start: 40
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178451
display id: annotation2178451
version: 1
top level: BBa_J202008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202008/annotation2178451/range2178451/1

https://synbiohub.org/public/igem/BBa_J202008/annotation2178451/range2178451/1
type: Range
persistent identity: range2178451
display id: range2178451
version: 1
direction: direction/forward
top level: BBa_J202008
start: 112
end: 112
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2178448
display id: annotation2178448
version: 1
top level: BBa_J202008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202008/annotation2178448/range2178448/1

https://synbiohub.org/public/igem/BBa_J202008/annotation2178448/range2178448/1
type: Range
persistent identity: range2178448
display id: range2178448
version: 1
direction: direction/forward
top level: BBa_J202008
start: 52
end: 52
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2178450
display id: annotation2178450
version: 1
top level: BBa_J202008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202008/annotation2178450/range2178450/1

https://synbiohub.org/public/igem/BBa_J202008/annotation2178450/range2178450/1
type: Range
persistent identity: range2178450
display id: range2178450
version: 1
direction: direction/forward
top level: BBa_J202008
start: 61
end: 77
orientation: inline

BBa_K773003
type: ComponentDefinition
persistent identity: BBa K773003
display id: BBa_K773003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-LVA
creator: Katie Knister
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
mutable notes: We modified the AAV sequence to LVA with PCR.
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Stop
sequence annotation: Start
sequence annotation: mCherry-LVA
sequence annotation: ssrA degradation tag: LVA
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K773003_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2209513
display id: annotation2209513
version: 1
top level: BBa_K773003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
type: Range
persistent identity: range2209513
display id: range2209513
version: 1
direction: direction/forward
top level: BBa_K773003
start: 763
end: 765
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209510
display id: annotation2209510
version: 1
top level: BBa_K773003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
type: Range
persistent identity: range2209510
display id: range2209510
version: 1
direction: direction/forward
top level: BBa_K773003
start: 19
end: 21
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209512
display id: annotation2209512
version: 1
top level: BBa_K773003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
type: Range
persistent identity: range2209512
display id: range2209512
version: 1
direction: direction/forward
top level: BBa_K773003
start: 760
end: 762
orientation: inline

mCherry-LVA
type: SequenceAnnotation
persistent identity: annotation2209509
display id: annotation2209509
version: 1
top level: BBa_K773003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
type: Range
persistent identity: range2209509
display id: range2209509
version: 1
direction: direction/forward
top level: BBa_K773003
start: 19
end: 765
orientation: inline

ssrA degradation tag: LVA
type: SequenceAnnotation
persistent identity: annotation2209511
display id: annotation2209511
version: 1
top level: BBa_K773003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
type: Range
persistent identity: range2209511
display id: range2209511
version: 1
direction: direction/forward
top level: BBa_K773003
start: 730
end: 759
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2209508
display id: annotation2209508
version: 1
top level: BBa_K773003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1

https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
type: Range
persistent identity: range2209508
display id: range2209508
version: 1
direction: direction/forward
top level: BBa_K773003
start: 1
end: 12
orientation: inline

BBa_K747001
type: ComponentDefinition
persistent identity: BBa K747001
display id: BBa_K747001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent pointmutation was inserted into the BsmBI restriction site in the chloramphenicol gene
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC1
sequence: https://synbiohub.org/public/igem/BBa_K747001_sequence/1

AC1
type: SequenceAnnotation
persistent identity: annotation2195755
display id: annotation2195755
version: 1
top level: BBa_K747001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747001/annotation2195755/range2195755/1

https://synbiohub.org/public/igem/BBa_K747001/annotation2195755/range2195755/1
type: Range
persistent identity: range2195755
display id: range2195755
version: 1
direction: direction/forward
top level: BBa_K747001
start: 12
end: 209
orientation: inline

BBa_K598020
type: ComponentDefinition
persistent identity: BBa K598020
display id: BBa_K598020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE
creator: Zhenrun ZHANG
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioAB+B0015+pBAD+TPP ribozyme 1.20+vioD+B0015+pBAD+vioE
mutable notes: The Ribosomal Binding Site of vioD is contained in TPP ribozyme 1.20.
mutable provenance: The hammerhead ribozyme comes from E. coli, and the TPP aptamer comes from B. subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: pBAD
sequence annotation: rbs
sequence annotation: vioB
sequence annotation: vioE
sequence annotation: TPP ribozyme 1.20
sequence annotation: vioA
sequence annotation: vioD
sequence: https://synbiohub.org/public/igem/BBa_K598020_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2154514
display id: annotation2154514
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1
type: Range
persistent identity: range2154514
display id: range2154514
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1
end: 129
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154577
display id: annotation2154577
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1
type: Range
persistent identity: range2154577
display id: range2154577
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6342
end: 6471
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154583
display id: annotation2154583
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1
type: Range
persistent identity: range2154583
display id: range2154583
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6205
end: 6333
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154575
display id: annotation2154575
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1
type: Range
persistent identity: range2154575
display id: range2154575
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4976
end: 4982
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154548
display id: annotation2154548
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1
type: Range
persistent identity: range2154548
display id: range2154548
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4702
end: 4831
orientation: inline

vioB
type: SequenceAnnotation
persistent identity: annotation2154542
display id: annotation2154542
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1
type: Range
persistent identity: range2154542
display id: range2154542
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1559
end: 4556
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154545
display id: annotation2154545
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1
type: Range
persistent identity: range2154545
display id: range2154545
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4565
end: 4693
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154518
display id: annotation2154518
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1
type: Range
persistent identity: range2154518
display id: range2154518
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1545
end: 1551
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154516
display id: annotation2154516
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1
type: Range
persistent identity: range2154516
display id: range2154516
version: 1
direction: direction/forward
top level: BBa_K598020
start: 273
end: 279
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154515
display id: annotation2154515
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1
type: Range
persistent identity: range2154515
display id: range2154515
version: 1
direction: direction/forward
top level: BBa_K598020
start: 138
end: 267
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2154586
display id: annotation2154586
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1
type: Range
persistent identity: range2154586
display id: range2154586
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6489
end: 7064
orientation: inline

TPP ribozyme 1.20
type: SequenceAnnotation
persistent identity: annotation2154556
display id: annotation2154556
version: 1
top level: BBa_K598020
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1
type: Range
persistent identity: range2154556
display id: range2154556
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4838
end: 4987
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2154517
display id: annotation2154517
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1
type: Range
persistent identity: range2154517
display id: range2154517
version: 1
direction: direction/forward
top level: BBa_K598020
start: 285
end: 1542
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154588
display id: annotation2154588
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1
type: Range
persistent identity: range2154588
display id: range2154588
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6477
end: 6483
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2154576
display id: annotation2154576
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1
type: Range
persistent identity: range2154576
display id: range2154576
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4988
end: 6112
orientation: inline

BBa_K779500
type: ComponentDefinition
persistent identity: BBa K779500
display id: BBa_K779500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long reporter top strand with bulge MammoBlock
creator: Kristjan Eerik Kaseniit
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1033_
m user id: 0
owner id: 12684
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter top strand with a 4nt bulge. Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts). IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmC mAmCmU mUmCmU mCmCmC mAmCmC mA/3AlexF488N/
mutable notes: Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. 4-nucleotide bulge added 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011). See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
mutable provenance: De novo design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Bulge
sequence annotation: Domain Sb
sequence annotation: Domain Sa part 1
sequence annotation: Domain Sa part 2
sequence: https://synbiohub.org/public/igem/BBa_K779500_sequence/1

Bulge
type: SequenceAnnotation
persistent identity: annotation2204231
display id: annotation2204231
version: 1
top level: BBa_K779500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779500/annotation2204231/range2204231/1

https://synbiohub.org/public/igem/BBa_K779500/annotation2204231/range2204231/1
type: Range
persistent identity: range2204231
display id: range2204231
version: 1
direction: direction/forward
top level: BBa_K779500
start: 11
end: 14
orientation: inline

Domain Sb
type: SequenceAnnotation
persistent identity: annotation2204232
display id: annotation2204232
version: 1
top level: BBa_K779500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779500/annotation2204232/range2204232/1

https://synbiohub.org/public/igem/BBa_K779500/annotation2204232/range2204232/1
type: Range
persistent identity: range2204232
display id: range2204232
version: 1
direction: direction/forward
top level: BBa_K779500
start: 1
end: 24
orientation: inline

Domain Sa part 1
type: SequenceAnnotation
persistent identity: annotation2204233
display id: annotation2204233
version: 1
top level: BBa_K779500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779500/annotation2204233/range2204233/1

https://synbiohub.org/public/igem/BBa_K779500/annotation2204233/range2204233/1
type: Range
persistent identity: range2204233
display id: range2204233
version: 1
direction: direction/forward
top level: BBa_K779500
start: 1
end: 10
orientation: inline

Domain Sa part 2
type: SequenceAnnotation
persistent identity: annotation2204234
display id: annotation2204234
version: 1
top level: BBa_K779500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779500/annotation2204234/range2204234/1

https://synbiohub.org/public/igem/BBa_K779500/annotation2204234/range2204234/1
type: Range
persistent identity: range2204234
display id: range2204234
version: 1
direction: direction/forward
top level: BBa_K779500
start: 15
end: 24
orientation: inline

BBa_K863001
type: ComponentDefinition
persistent identity: BBa K863001
display id: BBa_K863001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul laccase from Bacillus pumilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bpul (Laccase from Bacillus pumilus)
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: bpul
sequence annotation: ATG
sequence annotation: A (Original) - T (Mutation)
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863001_sequence/1

bpul
type: SequenceAnnotation
persistent identity: annotation2184621
display id: annotation2184621
version: 1
top level: BBa_K863001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1
type: Range
persistent identity: range2184621
display id: range2184621
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 1533
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184622
display id: annotation2184622
version: 1
top level: BBa_K863001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1
type: Range
persistent identity: range2184622
display id: range2184622
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 3
orientation: inline

A (Original) - T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184905
display id: annotation2184905
version: 1
top level: BBa_K863001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1
type: Range
persistent identity: range2184905
display id: range2184905
version: 1
direction: direction/forward
top level: BBa_K863001
start: 801
end: 801
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184641
display id: annotation2184641
version: 1
top level: BBa_K863001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1
type: Range
persistent identity: range2184641
display id: range2184641
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1531
end: 1533
orientation: inline

BBa_K747074
type: ComponentDefinition
persistent identity: BBa K747074
display id: BBa_K747074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 5
sequence: https://synbiohub.org/public/igem/BBa_K747074_sequence/1

GG 5
type: SequenceAnnotation
persistent identity: annotation2197043
display id: annotation2197043
version: 1
top level: BBa_K747074
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747074/annotation2197043/range2197043/1

https://synbiohub.org/public/igem/BBa_K747074/annotation2197043/range2197043/1
type: Range
persistent identity: range2197043
display id: range2197043
version: 1
direction: direction/forward
top level: BBa_K747074
start: 14
end: 211
orientation: inline

BBa_K808012
type: ComponentDefinition
persistent identity: BBa K808012
display id: BBa_K808012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphA2: Catalyzes together with tphA3 TPA to DCD
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808012_sequence/1

BBa_K747062
type: ComponentDefinition
persistent identity: BBa K747062
display id: BBa_K747062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 4
sequence: https://synbiohub.org/public/igem/BBa_K747062_sequence/1

TG 4
type: SequenceAnnotation
persistent identity: annotation2197019
display id: annotation2197019
version: 1
top level: BBa_K747062
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747062/annotation2197019/range2197019/1

https://synbiohub.org/public/igem/BBa_K747062/annotation2197019/range2197019/1
type: Range
persistent identity: range2197019
display id: range2197019
version: 1
direction: direction/forward
top level: BBa_K747062
start: 14
end: 211
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR repressor/activator, (no LVA?)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: Help:Barcodes
sequence annotation: A
sequence annotation: T
sequence annotation: BBa_C0062
sequence annotation: prefix
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
direction: direction/forward
top level: luxr
start: 757
end: 781
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
direction: direction/forward
top level: luxr
start: 492
end: 492
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
direction: direction/forward
top level: luxr
start: 174
end: 174
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 756
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 2
orientation: inline

BBa_K861110
type: ComponentDefinition
persistent identity: BBa K861110
display id: BBa_K861110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BcsB,regulator of cellulose synthetase
creator: Xian Xia
created: 2012-09-06T11:00:00Z
modified: 2015-06-17T01:21:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1121_
m user id: 0
m user id: 4206
owner id: 12357
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BcsB is involved in biosynthesis of cellulose, which is a significant constituent of the extracellular matrix observed during multicellular morphotype (rdar) growth. The BcsB protein that was indirectly inferred to bind c-di-GMP.
mutable notes: None
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K861110_sequence/1

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: SsrA
sequence annotation: CFP (LVA)
sequence annotation: BBa_E0022
sequence annotation: A
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
direction: direction/forward
top level: ECFP
start: 719
end: 756
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
direction: direction/forward
top level: ECFP
start: 69
end: 69
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
direction: direction/forward
top level: ECFP
start: 757
end: 762
orientation: inline

BBa_K876070
type: ComponentDefinition
persistent identity: BBa K876070
display id: BBa_K876070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CP-GFP-Term
creator: Zakaraya Ashour, Sanket Shah
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1138_
m user id: 0
owner id: 13835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter with GFP
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K081012
component: BBa_J23103
sequence annotation: BBa_K081012
sequence annotation: BBa_J23103
sequence: https://synbiohub.org/public/igem/BBa_K876070_sequence/1

BBa_K081012
type: Component
persistent identity: component2206867
display id: component2206867
version: 1
top level: BBa_K876070
access: public
definition: https://synbiohub.org/public/igem/BBa_K081012/1

BBa_J23103
type: Component
persistent identity: component2206856
display id: component2206856
version: 1
top level: BBa_K876070
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_K081012
type: SequenceAnnotation
persistent identity: annotation2206867
display id: annotation2206867
version: 1
top level: BBa_K876070
location: https://synbiohub.org/public/igem/BBa_K876070/annotation2206867/range2206867/1
component: BBa_K081012

https://synbiohub.org/public/igem/BBa_K876070/annotation2206867/range2206867/1
type: Range
persistent identity: range2206867
display id: range2206867
version: 1
direction: direction/reverse
top level: BBa_K876070
start: 44
end: 831
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2206856
display id: annotation2206856
version: 1
top level: BBa_K876070
location: https://synbiohub.org/public/igem/BBa_K876070/annotation2206856/range2206856/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K876070/annotation2206856/range2206856/1
type: Range
persistent identity: range2206856
display id: range2206856
version: 1
direction: direction/reverse
top level: BBa_K876070
start: 1
end: 35
orientation: inline

BBa_K779504
type: ComponentDefinition
persistent identity: BBa K779504
display id: BBa_K779504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long scrambled input strand MammoBlock
creator: Kristjan Eerik Kaseniit
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1033_
m user id: 0
owner id: 12684
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Long non-matching (scrambled) input strand to act as a negative control to the reporter formed by parts Part:BBa K779500 and Part:BBa K779502 or Part:BBa K779501 and Part:BBa K779502. Modifications: 5' IRD800 fluorophore, 3' phosphate. IDT DNA oligo order: /5IRD800/mAmC mUmAmA mCmCmU mAmAmC mUmAmC mAmAmC mAmAmC mAmCmU mAmUmC mCmA/3Phos/
mutable notes: In the domain, uses the same nucleotides as the Sa, but is scrambled so that the Sa and Sc domains are orthogonal. See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
mutable provenance: De novo design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Domain Sc
sequence annotation: Toehold
sequence: https://synbiohub.org/public/igem/BBa_K779504_sequence/1

Domain Sc
type: SequenceAnnotation
persistent identity: annotation2204257
display id: annotation2204257
version: 1
top level: BBa_K779504
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779504/annotation2204257/range2204257/1

https://synbiohub.org/public/igem/BBa_K779504/annotation2204257/range2204257/1
type: Range
persistent identity: range2204257
display id: range2204257
version: 1
direction: direction/forward
top level: BBa_K779504
start: 1
end: 20
orientation: inline

Toehold
type: SequenceAnnotation
persistent identity: annotation2204258
display id: annotation2204258
version: 1
top level: BBa_K779504
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779504/annotation2204258/range2204258/1

https://synbiohub.org/public/igem/BBa_K779504/annotation2204258/range2204258/1
type: Range
persistent identity: range2204258
display id: range2204258
version: 1
direction: direction/forward
top level: BBa_K779504
start: 21
end: 28
orientation: inline

BBa_K747006
type: ComponentDefinition
persistent identity: BBa K747006
display id: BBa_K747006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 1
sequence: https://synbiohub.org/public/igem/BBa_K747006_sequence/1

CG 1
type: SequenceAnnotation
persistent identity: annotation2195805
display id: annotation2195805
version: 1
top level: BBa_K747006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747006/annotation2195805/range2195805/1

https://synbiohub.org/public/igem/BBa_K747006/annotation2195805/range2195805/1
type: Range
persistent identity: range2195805
display id: range2195805
version: 1
direction: direction/forward
top level: BBa_K747006
start: 12
end: 209
orientation: inline

BBa_J202002
type: ComponentDefinition
persistent identity: BBa J202002
display id: BBa_J202002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAD Mutant#3
creator: Jiyeon Park
created: 2012-07-25T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
group u list: _910_
m user id: 0
owner id: 11164
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is PBAD (BBa_I13453) mutant. -71 relative to the araC transcriptional start site was changed from A to G. Also, C at -22 was changed to T
mutable notes: In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
mutable provenance: This mutant was created by random mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI2
sequence annotation: Mutation 2
sequence annotation: Mutation 1
sequence annotation: araI1
sequence annotation: Transcription Start
sequence: https://synbiohub.org/public/igem/BBa_J202002_sequence/1

araI2
type: SequenceAnnotation
persistent identity: annotation2178422
display id: annotation2178422
version: 1
top level: BBa_J202002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178422/range2178422/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178422/range2178422/1
type: Range
persistent identity: range2178422
display id: range2178422
version: 1
direction: direction/forward
top level: BBa_J202002
start: 61
end: 77
orientation: inline

Mutation 2
type: SequenceAnnotation
persistent identity: annotation2178425
display id: annotation2178425
version: 1
top level: BBa_J202002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178425/range2178425/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178425/range2178425/1
type: Range
persistent identity: range2178425
display id: range2178425
version: 1
direction: direction/forward
top level: BBa_J202002
start: 90
end: 90
orientation: inline

Mutation 1
type: SequenceAnnotation
persistent identity: annotation2178424
display id: annotation2178424
version: 1
top level: BBa_J202002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178424/range2178424/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178424/range2178424/1
type: Range
persistent identity: range2178424
display id: range2178424
version: 1
direction: direction/forward
top level: BBa_J202002
start: 41
end: 41
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2178421
display id: annotation2178421
version: 1
top level: BBa_J202002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178421/range2178421/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178421/range2178421/1
type: Range
persistent identity: range2178421
display id: range2178421
version: 1
direction: direction/forward
top level: BBa_J202002
start: 40
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2178423
display id: annotation2178423
version: 1
top level: BBa_J202002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J202002/annotation2178423/range2178423/1

https://synbiohub.org/public/igem/BBa_J202002/annotation2178423/range2178423/1
type: Range
persistent identity: range2178423
display id: range2178423
version: 1
direction: direction/forward
top level: BBa_J202002
start: 112
end: 112
orientation: inline

BBa_K561000
type: ComponentDefinition
persistent identity: BBa K561000
display id: BBa_K561000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pvgb+YFP+tetR
creator: HUA Yijie, PI Ruoxi, XIA Yu
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _729_
m user id: 0
owner id: 8637
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The expression of YFP and tetR is under the control of microaerobic promoter vgb. The expression of tetR will repress Ptet.
mutable notes: Because vgb promoter induces high expression under microaerobic conditions and induces lower expression under anaerobic conditions, we used medium RBS to resist it from inducing expression under anaerobic conditions.
mutable provenance: vgb promoter comes from Vitreoscilla sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K561001
component: BBa_P0440
component: BBa_I15017
sequence annotation: BBa_K561001
sequence annotation: BBa_I15017
sequence annotation: BBa_P0440
sequence: https://synbiohub.org/public/igem/BBa_K561000_sequence/1

BBa_K561001
type: Component
persistent identity: component2146962
display id: component2146962
version: 1
top level: BBa_K561000
access: public
definition: https://synbiohub.org/public/igem/BBa_K561001/1

BBa_P0440
type: Component
persistent identity: component2146980
display id: component2146980
version: 1
top level: BBa_K561000
access: public
definition: BBa_P0440

BBa_I15017
type: Component
persistent identity: component2146967
display id: component2146967
version: 1
top level: BBa_K561000
access: public
definition: https://synbiohub.org/public/igem/BBa_I15017/1

BBa_K561001
type: SequenceAnnotation
persistent identity: annotation2146962
display id: annotation2146962
version: 1
top level: BBa_K561000
location: https://synbiohub.org/public/igem/BBa_K561000/annotation2146962/range2146962/1
component: BBa_K561001

https://synbiohub.org/public/igem/BBa_K561000/annotation2146962/range2146962/1
type: Range
persistent identity: range2146962
display id: range2146962
version: 1
direction: direction/reverse
top level: BBa_K561000
start: 1
end: 135
orientation: inline

BBa_I15017
type: SequenceAnnotation
persistent identity: annotation2146967
display id: annotation2146967
version: 1
top level: BBa_K561000
location: https://synbiohub.org/public/igem/BBa_K561000/annotation2146967/range2146967/1
component: BBa_I15017

https://synbiohub.org/public/igem/BBa_K561000/annotation2146967/range2146967/1
type: Range
persistent identity: range2146967
display id: range2146967
version: 1
direction: direction/reverse
top level: BBa_K561000
start: 144
end: 885
orientation: inline

BBa_P0440
type: SequenceAnnotation
persistent identity: annotation2146980
display id: annotation2146980
version: 1
top level: BBa_K561000
location: https://synbiohub.org/public/igem/BBa_K561000/annotation2146980/range2146980/1
component: BBa_P0440

https://synbiohub.org/public/igem/BBa_K561000/annotation2146980/range2146980/1
type: Range
persistent identity: range2146980
display id: range2146980
version: 1
direction: direction/reverse
top level: BBa_K561000
start: 894
end: 1733
orientation: inline

BBa_K747030
type: ComponentDefinition
persistent identity: BBa K747030
display id: BBa_K747030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 2
sequence: https://synbiohub.org/public/igem/BBa_K747030_sequence/1

TG 2
type: SequenceAnnotation
persistent identity: annotation2196204
display id: annotation2196204
version: 1
top level: BBa_K747030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747030/annotation2196204/range2196204/1

https://synbiohub.org/public/igem/BBa_K747030/annotation2196204/range2196204/1
type: Range
persistent identity: range2196204
display id: range2196204
version: 1
direction: direction/forward
top level: BBa_K747030
start: 14
end: 211
orientation: inline

BBa_K909003
type: ComponentDefinition
persistent identity: BBa K909003
display id: BBa_K909003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cph8 (Cph1-Envz-Fusionprotein) with RBS
creator: Isaak Mueller
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14050
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K909002
sequence annotation: cph8 (cph1-envZ-fusion)
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: g->a
sequence: https://synbiohub.org/public/igem/BBa_K909003_sequence/1

BBa_K909002
type: SequenceAnnotation
persistent identity: annotation2194867
display id: annotation2194867
version: 1
top level: BBa_K909003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194867/range2194867/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194867/range2194867/1
type: Range
persistent identity: range2194867
display id: range2194867
version: 1
direction: direction/reverse
top level: BBa_K909003
start: 21
end: 2255
orientation: inline

cph8 (cph1-envZ-fusion)
type: SequenceAnnotation
persistent identity: annotation2194865
display id: annotation2194865
version: 1
top level: BBa_K909003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194865/range2194865/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194865/range2194865/1
type: Range
persistent identity: range2194865
display id: range2194865
version: 1
direction: direction/forward
top level: BBa_K909003
start: 21
end: 2255
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2194863
display id: annotation2194863
version: 1
top level: BBa_K909003
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194863/range2194863/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194863/range2194863/1
type: Range
persistent identity: range2194863
display id: range2194863
version: 1
direction: direction/forward
top level: BBa_K909003
start: 5
end: 8
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2194864
display id: annotation2194864
version: 1
top level: BBa_K909003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194864/range2194864/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194864/range2194864/1
type: Range
persistent identity: range2194864
display id: range2194864
version: 1
direction: direction/reverse
top level: BBa_K909003
start: 1
end: 12
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation2194866
display id: annotation2194866
version: 1
top level: BBa_K909003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K909003/annotation2194866/range2194866/1

https://synbiohub.org/public/igem/BBa_K909003/annotation2194866/range2194866/1
type: Range
persistent identity: range2194866
display id: range2194866
version: 1
direction: direction/forward
top level: BBa_K909003
start: 128
end: 128
orientation: inline

BBa_K747063
type: ComponentDefinition
persistent identity: BBa K747063
display id: BBa_K747063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 4
sequence: https://synbiohub.org/public/igem/BBa_K747063_sequence/1

TT 4
type: SequenceAnnotation
persistent identity: annotation2197020
display id: annotation2197020
version: 1
top level: BBa_K747063
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747063/annotation2197020/range2197020/1

https://synbiohub.org/public/igem/BBa_K747063/annotation2197020/range2197020/1
type: Range
persistent identity: range2197020
display id: range2197020
version: 1
direction: direction/forward
top level: BBa_K747063
start: 14
end: 211
orientation: inline

BBa_K863020
type: ComponentDefinition
persistent identity: BBa K863020
display id: BBa_K863020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans with T7 and HIS
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: T7
sequence annotation: RBS
sequence annotation: bhal
sequence annotation: ATG
sequence annotation: 6 x HIS
sequence: https://synbiohub.org/public/igem/BBa_K863020_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2202256
display id: annotation2202256
version: 1
top level: BBa_K863020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1
type: Range
persistent identity: range2202256
display id: range2202256
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1552
end: 1554
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2202251
display id: annotation2202251
version: 1
top level: BBa_K863020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1
type: Range
persistent identity: range2202251
display id: range2202251
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1
end: 23
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2202257
display id: annotation2202257
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1
type: Range
persistent identity: range2202257
display id: range2202257
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1555
end: 1557
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202252
display id: annotation2202252
version: 1
top level: BBa_K863020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1
type: Range
persistent identity: range2202252
display id: range2202252
version: 1
direction: direction/forward
top level: BBa_K863020
start: 25
end: 30
orientation: inline

bhal
type: SequenceAnnotation
persistent identity: annotation2202254
display id: annotation2202254
version: 1
top level: BBa_K863020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1
type: Range
persistent identity: range2202254
display id: range2202254
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 1533
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2202253
display id: annotation2202253
version: 1
top level: BBa_K863020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1
type: Range
persistent identity: range2202253
display id: range2202253
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 36
orientation: inline

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2202255
display id: annotation2202255
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1
type: Range
persistent identity: range2202255
display id: range2202255
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1534
end: 1551
orientation: inline

CMV AID pA
type: ComponentDefinition
persistent identity: BBa K929000
display id: BBa_K929000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AID with CMV promoter and hGH-polyadenylation signal sequence
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14457
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K103001). It is built of 3 parts: CMV promoter, AID and hGH-polyA tail. AID: AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes. CMV promoter: CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter. hGH-polyA tail: The polyA tail consists of multiple adenosine monophosphates. hGH-polyA tail is a significant part for the translation and stability of mRNA. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.
mutable notes: bla bla
mutable provenance: It is a composite part consisting of 3 other Biobricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: AID
sequence annotation: UGA
sequence annotation: BBa_K404108
sequence annotation: BBa_I712004
sequence annotation: BBa_K103001
sequence annotation: NcoI
sequence annotation: AUG
sequence annotation: CMV promoter
sequence annotation: hGH terminator
sequence: https://synbiohub.org/public/igem/BBa_K929000_sequence/1

AID
type: SequenceAnnotation
persistent identity: annotation2197699
display id: annotation2197699
version: 1
top level: CMV AID pA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197699/range2197699/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197699/range2197699/1
type: Range
persistent identity: range2197699
display id: range2197699
version: 1
direction: direction/forward
top level: CMV AID pA
start: 665
end: 1262
orientation: inline

UGA
type: SequenceAnnotation
persistent identity: annotation2197701
display id: annotation2197701
version: 1
top level: CMV AID pA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197701/range2197701/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197701/range2197701/1
type: Range
persistent identity: range2197701
display id: range2197701
version: 1
direction: direction/forward
top level: CMV AID pA
start: 1259
end: 1262
orientation: inline

BBa_K404108
type: SequenceAnnotation
persistent identity: annotation2197703
display id: annotation2197703
version: 1
top level: CMV AID pA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197703/range2197703/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197703/range2197703/1
type: Range
persistent identity: range2197703
display id: range2197703
version: 1
direction: direction/reverse
top level: CMV AID pA
start: 1276
end: 1754
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2197696
display id: annotation2197696
version: 1
top level: CMV AID pA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197696/range2197696/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197696/range2197696/1
type: Range
persistent identity: range2197696
display id: range2197696
version: 1
direction: direction/reverse
top level: CMV AID pA
start: 1
end: 654
orientation: inline

BBa_K103001
type: SequenceAnnotation
persistent identity: annotation2197698
display id: annotation2197698
version: 1
top level: CMV AID pA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197698/range2197698/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197698/range2197698/1
type: Range
persistent identity: range2197698
display id: range2197698
version: 1
direction: direction/reverse
top level: CMV AID pA
start: 663
end: 1262
orientation: inline

NcoI
type: SequenceAnnotation
persistent identity: annotation2197697
display id: annotation2197697
version: 1
top level: CMV AID pA
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197697/range2197697/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197697/range2197697/1
type: Range
persistent identity: range2197697
display id: range2197697
version: 1
direction: direction/forward
top level: CMV AID pA
start: 663
end: 668
orientation: inline

AUG
type: SequenceAnnotation
persistent identity: annotation2197700
display id: annotation2197700
version: 1
top level: CMV AID pA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197700/range2197700/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197700/range2197700/1
type: Range
persistent identity: range2197700
display id: range2197700
version: 1
direction: direction/forward
top level: CMV AID pA
start: 665
end: 667
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2197695
display id: annotation2197695
version: 1
top level: CMV AID pA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197695/range2197695/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197695/range2197695/1
type: Range
persistent identity: range2197695
display id: range2197695
version: 1
direction: direction/forward
top level: CMV AID pA
start: 1
end: 654
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2197702
display id: annotation2197702
version: 1
top level: CMV AID pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K929000/annotation2197702/range2197702/1

https://synbiohub.org/public/igem/BBa_K929000/annotation2197702/range2197702/1
type: Range
persistent identity: range2197702
display id: range2197702
version: 1
direction: direction/reverse
top level: CMV AID pA
start: 1276
end: 1754
orientation: inline

BBa_K747090
type: ComponentDefinition
persistent identity: BBa K747090
display id: BBa_K747090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 6
sequence: https://synbiohub.org/public/igem/BBa_K747090_sequence/1

GG 6
type: SequenceAnnotation
persistent identity: annotation2197066
display id: annotation2197066
version: 1
top level: BBa_K747090
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747090/annotation2197066/range2197066/1

https://synbiohub.org/public/igem/BBa_K747090/annotation2197066/range2197066/1
type: Range
persistent identity: range2197066
display id: range2197066
version: 1
direction: direction/forward
top level: BBa_K747090
start: 14
end: 211
orientation: inline

BBa_K747089
type: ComponentDefinition
persistent identity: BBa K747089
display id: BBa_K747089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 6
sequence: https://synbiohub.org/public/igem/BBa_K747089_sequence/1

GC 6
type: SequenceAnnotation
persistent identity: annotation2197065
display id: annotation2197065
version: 1
top level: BBa_K747089
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747089/annotation2197065/range2197065/1

https://synbiohub.org/public/igem/BBa_K747089/annotation2197065/range2197065/1
type: Range
persistent identity: range2197065
display id: range2197065
version: 1
direction: direction/forward
top level: BBa_K747089
start: 14
end: 211
orientation: inline

BBa_K733018
type: ComponentDefinition
persistent identity: BBa K733018
display id: BBa_K733018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylR+PxylA+RBS+GFP+Double Terminator
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K733002
component: BBa_E0240
sequence annotation: BBa_K733002
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K733018_sequence/1

BBa_K733002
type: Component
persistent identity: component2183842
display id: component2183842
version: 1
top level: BBa_K733018
access: public
definition: BBa_K733002

BBa_E0240
type: Component
persistent identity: component2183853
display id: component2183853
version: 1
top level: BBa_K733018
access: public
definition: GFP report

BBa_K733002
type: SequenceAnnotation
persistent identity: annotation2183842
display id: annotation2183842
version: 1
top level: BBa_K733018
location: https://synbiohub.org/public/igem/BBa_K733018/annotation2183842/range2183842/1
component: BBa_K733002

https://synbiohub.org/public/igem/BBa_K733018/annotation2183842/range2183842/1
type: Range
persistent identity: range2183842
display id: range2183842
version: 1
direction: direction/reverse
top level: BBa_K733018
start: 1
end: 1387
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2183853
display id: annotation2183853
version: 1
top level: BBa_K733018
location: https://synbiohub.org/public/igem/BBa_K733018/annotation2183853/range2183853/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K733018/annotation2183853/range2183853/1
type: Range
persistent identity: range2183853
display id: range2183853
version: 1
direction: direction/reverse
top level: BBa_K733018
start: 1396
end: 2271
orientation: inline

BBa_K880002
type: ComponentDefinition
persistent identity: BBa K880002
display id: BBa_K880002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -IRR K137008 + inverted tetR promoter K137047 + IRL K137010 + GFP I13504 (MI275) -GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases. Same components as K137058 with the inverted repeats reversed such that the external half sites are external to the flip region (IRR - ???flip region??? - IRL) GFP fluorescent reporter capable of assaying the functionality of fim regulatory recombinases; based on K137058. K137058???s invertible repeat sequences responsible for recombinase binding are ordered incorrectly; the regions are not identical, and have ???left??? and ???right??? components. K137008 (invertible repeat-right) and K137010 (invertible repeat-left) are mislabeled and belong in the order ???K137008-region to invert-K137010??? in accordance with the wild-type fim system. Repeat sequence in this part exist in the correct (wild-type) orientation.
mutable notes: Enter design considerations
mutable provenance: Enter source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K137008
component: BBa_K137047
component: BBa_K137010
component: BBa_I13504
sequence annotation: BBa_K137010
sequence annotation: BBa_K137047
sequence annotation: BBa_K137008
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K880002_sequence/1

BBa_K137008
type: Component
persistent identity: component2204543
display id: component2204543
version: 1
top level: BBa_K880002
access: public
definition: https://synbiohub.org/public/igem/BBa_K137008/1

BBa_K137047
type: Component
persistent identity: component2204545
display id: component2204545
version: 1
top level: BBa_K880002
access: public
definition: https://synbiohub.org/public/igem/BBa_K137047/1

BBa_K137010
type: Component
persistent identity: component2204548
display id: component2204548
version: 1
top level: BBa_K880002
access: public
definition: https://synbiohub.org/public/igem/BBa_K137010/1

BBa_I13504
type: Component
persistent identity: component2204558
display id: component2204558
version: 1
top level: BBa_K880002
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation2204548
display id: annotation2204548
version: 1
top level: BBa_K880002
location: https://synbiohub.org/public/igem/BBa_K880002/annotation2204548/range2204548/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K880002/annotation2204548/range2204548/1
type: Range
persistent identity: range2204548
display id: range2204548
version: 1
direction: direction/reverse
top level: BBa_K880002
start: 302
end: 336
orientation: inline

BBa_K137047
type: SequenceAnnotation
persistent identity: annotation2204545
display id: annotation2204545
version: 1
top level: BBa_K880002
location: https://synbiohub.org/public/igem/BBa_K880002/annotation2204545/range2204545/1
component: BBa_K137047

https://synbiohub.org/public/igem/BBa_K880002/annotation2204545/range2204545/1
type: Range
persistent identity: range2204545
display id: range2204545
version: 1
direction: direction/reverse
top level: BBa_K880002
start: 44
end: 293
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation2204543
display id: annotation2204543
version: 1
top level: BBa_K880002
location: https://synbiohub.org/public/igem/BBa_K880002/annotation2204543/range2204543/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K880002/annotation2204543/range2204543/1
type: Range
persistent identity: range2204543
display id: range2204543
version: 1
direction: direction/reverse
top level: BBa_K880002
start: 1
end: 35
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2204558
display id: annotation2204558
version: 1
top level: BBa_K880002
location: https://synbiohub.org/public/igem/BBa_K880002/annotation2204558/range2204558/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K880002/annotation2204558/range2204558/1
type: Range
persistent identity: range2204558
display id: range2204558
version: 1
direction: direction/reverse
top level: BBa_K880002
start: 345
end: 1219
orientation: inline

BBa_S0108
type: ComponentDefinition
persistent identity: BBa S0108
display id: BBa_S0108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0033.C0050
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0050
component: BBa_B0033
sequence annotation: BBa_C0050
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_S0108_sequence/1

BBa_C0050
type: Component
persistent identity: component939848
display id: component939848
version: 1
top level: BBa_S0108
access: public
definition: https://synbiohub.org/public/igem/BBa_C0050/1

BBa_B0033
type: Component
persistent identity: component939832
display id: component939832
version: 1
top level: BBa_S0108
access: public
definition: BBa_B0033

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation939848
display id: annotation939848
version: 1
top level: BBa_S0108
location: https://synbiohub.org/public/igem/BBa_S0108/annotation939848/range939848/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_S0108/annotation939848/range939848/1
type: Range
persistent identity: range939848
display id: range939848
version: 1
direction: direction/forward
top level: BBa_S0108
start: 18
end: 761
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939832
display id: annotation939832
version: 1
top level: BBa_S0108
location: https://synbiohub.org/public/igem/BBa_S0108/annotation939832/range939832/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_S0108/annotation939832/range939832/1
type: Range
persistent identity: range939832
display id: range939832
version: 1
direction: direction/forward
top level: BBa_S0108
start: 1
end: 11
orientation: inline

BBa_I6044
type: ComponentDefinition
persistent identity: BBa I6044
display id: BBa_I6044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test R0063.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-16T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_R0063
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_R0063
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I6044_sequence/1

BBa_B0010
type: Component
persistent identity: component948586
display id: component948586
version: 1
top level: BBa_I6044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component948579
display id: component948579
version: 1
top level: BBa_I6044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0063
type: Component
persistent identity: component948570
display id: component948570
version: 1
top level: BBa_I6044
access: public
definition: lux pL

BBa_B0012
type: Component
persistent identity: component948596
display id: component948596
version: 1
top level: BBa_I6044
access: public
definition: BBa_B0012

BBa_E0030
type: Component
persistent identity: component948581
display id: component948581
version: 1
top level: BBa_I6044
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948579
display id: annotation948579
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948579/range948579/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6044/annotation948579/range948579/1
type: Range
persistent identity: range948579
display id: range948579
version: 1
direction: direction/forward
top level: BBa_I6044
start: 160
end: 171
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation948570
display id: annotation948570
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948570/range948570/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I6044/annotation948570/range948570/1
type: Range
persistent identity: range948570
display id: range948570
version: 1
direction: direction/forward
top level: BBa_I6044
start: 1
end: 151
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948596
display id: annotation948596
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948596/range948596/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6044/annotation948596/range948596/1
type: Range
persistent identity: range948596
display id: range948596
version: 1
direction: direction/forward
top level: BBa_I6044
start: 997
end: 1037
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948586
display id: annotation948586
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948586/range948586/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6044/annotation948586/range948586/1
type: Range
persistent identity: range948586
display id: range948586
version: 1
direction: direction/forward
top level: BBa_I6044
start: 909
end: 988
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948581
display id: annotation948581
version: 1
top level: BBa_I6044
location: https://synbiohub.org/public/igem/BBa_I6044/annotation948581/range948581/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6044/annotation948581/range948581/1
type: Range
persistent identity: range948581
display id: range948581
version: 1
direction: direction/forward
top level: BBa_I6044
start: 178
end: 900
orientation: inline