Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.
70 bp from Eco to Spe or Xba to Pst, to allow for gel extraction. The middle portion is largely noise, intended as filler to raise the size of the part.
This part accepts POPS to generate EYFP under the medium strength RBS B0032.
Lac operator with CFP reporter (without LVA tag) [R/Lc-]
Christopher Batten, Victoria Chou, Kenneth Nesmith
Built by Josh as an intermediate in screening plasmid construction.
This part accepts POPS to produce ECFP under the medium RBS B0032
Built by Josh as an intermediate in screening plasmid construction
Tet operator with CFP reporter (with LVA tag) [R/Tc+]