iGEM_2019_Plasmid_pSB1A2
1
iGEM 2019 Plasmid pSB1A2
BBa_J61001
1
R6K
[R6K] Origin of replication
John Anderson
2006-07-30T11:00:00Z
2015-08-31T02:02:59Z
false
false
_95_
0
483
95
Released HQ 2013
In stock
false
R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature.
{pSB1A2-Bca1011}
N/A
<tt>
PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI)<br>
Sub into pSB1A2-I13522 (EcoRI/SpeI)<br>
Product is Bca1011<br>
----
ca1011F Forward Biobricking of R6K<br>
GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg<br>
ca1011R Reverse biobricking of R6K<br>
ccgctactagtaGCAGTTCAACCTGTTGATAG<br>
</tt>
true
annotation1892271
1
R6K
range1892271
1
27
406
BBa_I13504
1
BBa_I13504
Screening plasmid intermediate
jkm
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
false
true
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction.
true
component1505067
1
BBa_B0034
component1505069
1
BBa_E0040
component1505084
1
BBa_B0012
component1505074
1
BBa_B0010
annotation1505069
1
BBa_E0040
range1505069
1
19
738
annotation1505067
1
BBa_B0034
range1505067
1
1
12
annotation1505074
1
BBa_B0010
range1505074
1
747
826
annotation1505084
1
BBa_B0012
range1505084
1
835
875
BBa_R0051
1
cI lam
promoter (lambda cI regulated)
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P>
<P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor.
<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
true
annotation2023
1
-35
range2023
1
15
20
annotation2022
1
-10
range2022
1
38
43
annotation2024
1
OR1
range2024
1
25
41
annotation2025
1
OR2
range2025
1
1
17
annotation7067
1
BBa_R0051
range7067
1
1
49
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
<em>V. fischeri</em> <genbank>AF170104</genbank>
true
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
annotation1765
1
A
range1765
1
492
492
annotation1766
1
luxR
range1766
1
1
750
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation1764
1
T
range1764
1
174
174
annotation1762
1
prefix
range1762
1
1
2
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
represillator of Elowitz and Leibler (2000)
true
annotation2001
1
lac O1
range2001
1
26
42
annotation2002
1
-10
range2002
1
43
48
annotation1999
1
lac O1
range1999
1
3
19
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2000
1
-35
range2000
1
20
25
BBa_C0012
1
lacI
lacI repressor from E. coli (+LVA)
Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.</P> <P>A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.</P>
References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P> References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P>Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.<P> Incompatible with systems containing LacI, lactose, or IPTG.
represillator of Elowitz and Leibler (2000)
true
annotation1723
1
lacI-LVA
range1723
1
1
1128
annotation2213988
1
Help:Barcodes
range2213988
1
1129
1153
annotation7031
1
BBa_C0012
range7031
1
1
1128
annotation1722
1
LVA
range1722
1
1090
1128
BBa_R0071
1
RhlR+C4
Promoter (RhlR & C4-HSL regulated)
Ronny Krashinsky
2004-01-24T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.
<p>
This is the natural sequence taken from Pseudomonas aeruginosa.
<p>
Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
The -10 and -35 sites are labeled as identified in [Pearson97].
<P>
The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
<i>Pseudomonas aeruginosa</i> rhlAB promoter
true
annotation297105
1
start
range297105
1
53
53
annotation297103
1
-35
range297103
1
19
24
annotation297104
1
-10
range297104
1
42
47
annotation297102
1
RhlR
range297102
1
1
20
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
annotation23316
1
conserved
range23316
1
7
10
BBa_J61101
1
BBa_J61101
Ribosome Binding Site Family Member
John Anderson
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
false
false
_95_
0
483
95
In stock
false
fix
N/A
N/A
true
BBa_C0051
1
cI lam
cI repressor from E. coli phage lambda (+LVA)
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).</P>
References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P> References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P>BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.<P>
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
true
annotation2213991
1
Help:Barcodes
range2213991
1
751
775
annotation23335
1
LVA
range23335
1
712
744
annotation23334
1
cI lambda
range23334
1
4
711
BBa_I715023
1
RFP2
Carboxyl portion of RFP
Andrew Martens
2007-06-24T11:00:00Z
2016-01-25T04:44:49Z
false
false
_120_
4206
201
61
In stock
false
The second portion of the RFP gene, after the HixC insertion point.
Used web tool.
Sequence from part BBa_E1010.
true
annotation1935383
1
Continue coding sequence
range1935383
1
2
214
annotation1935382
1
extra base (avoid frameshift)
range1935382
1
1
1
annotation1935384
1
(two in tandem)
range1935384
1
215
219
BBa_E0430
1
BBa_E0430
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
Caitlin Conboy
2004-03-15T12:00:00Z
2015-08-31T04:07:26Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Standard YFP Output Device -LVA tag
true
component942902
1
BBa_B0012
component942887
1
BBa_E0030
component942892
1
BBa_B0010
component942885
1
BBa_B0034
annotation942902
1
BBa_B0012
range942902
1
838
878
annotation942887
1
BBa_E0030
range942887
1
19
741
annotation942892
1
BBa_B0010
range942892
1
750
829
annotation942885
1
BBa_B0034
range942885
1
1
12
BBa_E0026
1
ECFP
enhanced cyan fluorescent protein derived from A. victoria GFP
jcbraff
2004-06-06T11:00:00Z
2015-08-31T04:07:25Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
-- No description --
true
BBa_S0105
1
BBa_S0105
B0034.C0053
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-05-08T01:14:17Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
true
component939789
1
BBa_C0053
component939777
1
BBa_B0034
annotation939777
1
BBa_B0034
range939777
1
1
12
annotation939789
1
BBa_C0053
range939789
1
19
705
BBa_B0024
1
BBa_B0024
double terminator (B0012-B0011), reversed
Caitlin Conboy
2003-12-02T12:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
In stock
false
-- No description --
true
BBa_C0050
1
cI HK022
cI repressor from phage HK022 (+LVA?)
Reshma Shetty
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.</P>
References (unparsed) here: <p>Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30.<br> PMID: 8487297.</P> <P> Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor.<br> J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698.</P> <p></p> <p></p> <P> References (unparsed) here: <p>Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30.<br> PMID: 8487297.</P> <P> Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor.<br> J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698.</P> <p></p> <p></p> <P>Derived from <genbank>STHK022N</genbank>.<br> <br> Response from John Little (Arizona) regarding the start of HK022 cI<br> <br> From: <jlittle@email.arizona.edu> <br> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern <br> To: "Drew Endy" <endy@MIT.EDU><br> Subject: RE: hk022 cI start (naive question)? <br> Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. <br> Good luck <br> John<br> <br> -- Original Message -- <br> Date: Sun, 19 Jan 2003 15:26:26 -0500 <br> Subject: hk022 cI start (naive question)? <br> Cc: elowitm@rockefeller.edu <br> To: jlittle@u.arizona.edu <br> From: Drew Endy <endy@MIT.EDU> <br> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It looks like this extra DNA extends into the PR regulatory region. We're wondering what's going on here? I'm guessing this is well documented somewhere. Thanks for any pointers/info! <br> Drew<P> It is unknown whether there is cross-talk between this repressor and Lambda cI regulatory region, 434 cI regulatory region and P22 regulatory region.
Bacteriophage HK022.
true
annotation2213990
1
Help:Barcodes
range2213990
1
745
769
annotation1736
1
SsrA
range1736
1
706
738
annotation1735
1
cI HK022
range1735
1
1
744
annotation1737
1
OR3 partial
range1737
1
1
8
annotation1734
1
2
range1734
1
739
744
annotation7033
1
BBa_C0050
range7033
1
1
744
BBa_P0152
1
BBa_P0152
PoPS -> cI (434) [S0166]
Randy Rettberg
2004-04-24T11:00:00Z
2015-05-08T01:14:10Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Protein generator converting TIPS to the protein cI from 434. Used as the input section for Quad Part Inverter Q01520.
true
component944545
1
BBa_C0052
component944552
1
BBa_B0010
component944562
1
BBa_B0012
component944535
1
BBa_B0031
annotation944562
1
BBa_B0012
range944562
1
811
851
annotation944552
1
BBa_B0010
range944552
1
723
802
annotation944545
1
BBa_C0052
range944545
1
21
689
annotation944535
1
BBa_B0031
range944535
1
1
14
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
<em>V. fischeri</em>
true
annotation2045
1
LuxR/HSL
range2045
1
1
20
annotation2046
1
-35
range2046
1
20
25
annotation2047
1
-10
range2047
1
42
47
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2048
1
start
range2048
1
53
53
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_C0053
1
c2 P22
c2 repressor from Salmonella phage P22 (+LVA)
Maia Mahoney
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.</p>
References (unparsed) here: <p>Vander Byl,C. and Kropinski,A.M. <em>Sequence of the genome of Salmonella bacteriophage P22</em><br> J. Bacteriol. 182 (22), 6472-6481 (2000) <P> References (unparsed) here: <p>Vander Byl,C. and Kropinski,A.M. <em>Sequence of the genome of Salmonella bacteriophage P22</em><br> J. Bacteriol. 182 (22), 6472-6481 (2000) <P><P>
Bacteriophage P22
true
annotation1750
1
LVA
range1750
1
649
681
annotation1751
1
stop
range1751
1
682
687
annotation1747
1
cII p22
range1747
1
1
648
annotation2213993
1
Help:Barcodes
range2213993
1
688
712
annotation7036
1
BBa_C0053
range7036
1
1
687
BBa_C0040
1
tetR
tetracycline repressor from transposon Tn10 (+LVA)
June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P>
References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
true
annotation23330
1
SsrA
range23330
1
621
654
annotation2213989
1
Help:Barcodes
range2213989
1
661
685
annotation23329
1
tetR
range23329
1
4
620
BBa_C0061
1
luxI
autoinducer synthetase for AHL
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Synthesizes 3OC<sub>6</sub>HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
<P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P>
<em>V. fischeri</em> <genbank>AF170104</genbank>
true
annotation1760
1
LVA
range1760
1
580
611
annotation7038
1
BBa_C0061
range7038
1
1
618
annotation2213985
1
Help:Barcodes
range2213985
1
619
643
annotation1761
1
luxI
range1761
1
1
579
BBa_I15017
1
ecfp
B0032.EYFP
Jeff Tabor
2005-04-14T11:00:00Z
2015-08-31T04:07:38Z
false
true
_5_
0
88
5
Released HQ 2013
In stock
false
This part accepts POPS to generate EYFP under the medium strength RBS B0032.
true
component1478422
1
BBa_E0030
component1478419
1
BBa_B0032
annotation1478422
1
BBa_E0030
range1478422
1
20
742
annotation1478419
1
BBa_B0032
range1478419
1
1
13
BBa_J61100
1
BBa_J61100
Ribosome Binding Site Family Member
John Anderson
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
false
true
_95_
0
483
95
In stock
false
{{JCA_Arkin_RBSFamily}}
N/A
N/A
true
BBa_I13502
1
mRFP1
Screening plasmid intermediate
jkm
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction.
true
component1505038
1
BBa_B0034
component1505045
1
BBa_E1010
annotation1505038
1
BBa_B0034
range1505038
1
1
12
annotation1505045
1
BBa_E1010
range1505045
1
19
699
BBa_R0080
1
AraC
Promoter (AraC regulated)
Sara Neves (Fighting Darwins)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
GenBank: J01641 (www.ncbi.nlm.nih.gov)
true
annotation301462
1
ara1 and ara2
range301462
1
73
101
annotation308601
1
-35
range308601
1
113
118
annotation308602
1
-10
range308602
1
136
141
annotation301456
1
c-amp2
range301456
1
4
29
annotation301458
1
c-amp1
range301458
1
43
72
annotation301457
1
araO1
range301457
1
6
44
BBa_R0079
1
LasR+PAI
Promoter (LasR & PAI regulated)
Alvin Carter Powers (Fighting Darwins)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Binding region for LasR protein (positive regulation)
"Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
true
annotation300992
1
OP1
range300992
1
106
125
annotation318496
1
-10
range318496
1
140
145
annotation300985
1
OP2
range300985
1
46
63
annotation318495
1
-35
range318495
1
117
122
BBa_E0024
1
ECFP
enhanced cyan fluorescent protein derived from A. victoria GFP
jcbraff
2004-06-06T11:00:00Z
2015-08-31T04:07:25Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
-- No description --
true
BBa_E0022
1
ECFP
enhanced cyan fluorescent protein derived from A. victoria GFP
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
2003-01-31T12:00:00Z
2015-08-31T04:07:25Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. </P>
<P> <P>BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
Modified from <bb_part>BBa_E0021</bb_part>.
true
annotation2153
1
SsrA
range2153
1
719
756
annotation2150
1
CFP (LVA)
range2150
1
1
762
annotation2154
1
2
range2154
1
757
762
annotation7040
1
BBa_E0022
range7040
1
1
762
annotation2155
1
A
range2155
1
69
69
BBa_I13601
1
BBa_I13601
Lac operator with CFP reporter (without LVA tag) [R/Lc-]
Christopher Batten, Victoria Chou, Kenneth Nesmith
2004-07-15T11:00:00Z
2015-08-31T04:07:36Z
false
true
_6_
0
101
7
It's complicated
false
-- No description --
true
component2221477
1
BBa_E0420
component2221463
1
BBa_R0011
annotation2221463
1
BBa_R0011
range2221463
1
1
54
annotation2221477
1
BBa_E0420
range2221477
1
64
941
BBa_B0025
1
BBa_B0025
double terminator (B0015), reversed
Caitlin Conboy
2003-12-02T12:00:00Z
2015-08-31T04:07:20Z
false
true
_1_
0
24
7
In stock
false
-- No description --
true
annotation369702
1
B0012
range369702
1
1
41
annotation369703
1
B0010
range369703
1
50
129
BBa_J61000
1
CmR
chloramphenicol resistance cassette
John Anderson
2006-07-30T11:00:00Z
2015-08-31T02:02:59Z
false
false
_95_
0
483
95
It's complicated
false
Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol.
{pSB1A2-Bca1016}
N/A
<tt>
PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI)<br>
Sub into pSB1A2-I13522 (EcoRI/SpeI)<br>
Products are Bca1015 and Bca1016<br>
----
ca1016F Forward EcoRI to biobrick CAT of pKD3<br>
gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg<br>
ca1016R Reverse SpeI to biobrick CAT of pKD3<br>
ccgctactagtggcgcgcctacctgtgacgg<br>
</tt>
true
annotation1892270
1
CmR
range1892270
1
30
687
BBa_S03119
1
BBa_S03119
--Specify Parts List--
Randy Rettberg
2004-04-19T11:00:00Z
2015-05-08T01:14:19Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Intermediate part from assembly 299
true
component955067
1
BBa_B0012
component955026
1
BBa_R0040
component955057
1
BBa_B0010
component955034
1
BBa_B0034
component955049
1
BBa_C0062
annotation955067
1
BBa_B0012
range955067
1
958
998
annotation955026
1
BBa_R0040
range955026
1
1
54
annotation955034
1
BBa_B0034
range955034
1
63
74
annotation955057
1
BBa_B0010
range955057
1
870
949
annotation955049
1
BBa_C0062
range955049
1
81
836
BBa_I13401
1
BBa_I13401
GFP reporter for RHS of library test constructs
jasonk
2005-04-05T11:00:00Z
2015-08-31T04:07:33Z
false
true
_6_
0
2
6
Released HQ 2013
In stock
false
This part will be suffixed to a promoter.RBS library. The purpose of the experiment is to make a first pass at a library-based construction step to identify efficiency issues and also to examine maxPOPS/RIPS level.
This part was built by Jen as an intermediate to their nuts and bolts parts.
true
component1476718
1
BBa_B0010
component1476713
1
BBa_E0040
component1476728
1
BBa_B0012
annotation1476713
1
BBa_E0040
range1476713
1
1
720
annotation1476728
1
BBa_B0012
range1476728
1
817
857
annotation1476718
1
BBa_B0010
range1476718
1
729
808
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
Reshma Shetty
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
true
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_R0063
1
lux pL
Promoter (luxR & HSL regulated -- lux pL)<br>
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl</p>
<P> <P> This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <p><img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"><P>Unspecified.
<em>V. fischeri.</em>
true
annotation2053
1
-35
range2053
1
89
94
annotation2054
1
start
range2054
1
128
128
annotation7071
1
BBa_R0063
range7071
1
1
151
annotation2052
1
-10
range2052
1
115
122
annotation2055
1
Putative LuxR/HSL
range2055
1
130
149
annotation2051
1
LuxR/HSL
range2051
1
1
20
BBa_I13002
1
BBa_I13002
RBS B0031 w/ YFP (-LVA) TT
Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
2004-06-27T11:00:00Z
2015-08-31T04:07:32Z
false
false
_6_
0
101
7
Released HQ 2013
In stock
false
-- No description --
true
component943452
1
BBa_B0031
component943454
1
BBa_E0030
component943469
1
BBa_B0012
component943459
1
BBa_B0010
annotation943454
1
BBa_E0030
range943454
1
21
743
annotation943459
1
BBa_B0010
range943459
1
752
831
annotation943452
1
BBa_B0031
range943452
1
1
14
annotation943469
1
BBa_B0012
range943469
1
840
880
BBa_R0083
1
OmpR
Promoter (OmpR, positive)
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown.
The a at base 41 is mutated to t to avoid XbaI
site. Speculation that this was a previous assembly site.
NC_000193 E. coli K12
true
annotation301223
1
linker
range301223
1
37
44
annotation301224
1
-35
range301224
1
45
50
annotation301221
1
C1 OmpR
range301221
1
13
30
annotation306881
1
A
range306881
1
41
41
annotation301226
1
-10
range301226
1
68
73
BBa_R0084
1
OmpR
Promoter (OmpR, positive)
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
NC_000913 E. coli K12
true
annotation301313
1
F3 OmpR
range301313
1
51
68
annotation301309
1
F2 OmpR
range301309
1
31
48
annotation301308
1
F1 OmpR
range301308
1
11
28
annotation301318
1
-10
range301318
1
88
93
annotation301317
1
-35
range301317
1
73
78
BBa_E0020
1
ecfp
engineered cyan fluorescent protein derived from A. victoria GFP
Caitlin Conboy and Jennifer Braff
2004-03-02T12:00:00Z
2015-08-31T04:07:25Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
true
BBa_E0422
1
BBa_E0422
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-08-31T04:07:26Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
true
component942803
1
BBa_B0034
component942815
1
BBa_E0022
component942833
1
BBa_B0012
component942823
1
BBa_B0010
annotation942833
1
BBa_B0012
range942833
1
877
917
annotation942823
1
BBa_B0010
range942823
1
789
868
annotation942803
1
BBa_B0034
range942803
1
1
12
annotation942815
1
BBa_E0022
range942815
1
19
780
BBa_C0056
1
BBa_C0056
cI repressor from phage 434 (no LVA)
Reshma Shetty
2004-09-01T11:00:00Z
2015-08-31T04:07:23Z
false
false
_41_6_
0
126
7
Released HQ 2013
In stock
false
-- No description --
Removed LVA tag from BBa_C0052 via PCR.
In pSB1AK3 currently.
Removed LVA tag from BBa_C0052
true
annotation1063931
1
cI 434
range1063931
1
1
636
annotation1063930
1
2
range1063930
1
631
636
BBa_C0052
1
cI 434
cI repressor from phage 434 (+LVA)
Maia Mahoney
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.</p>
References (unparsed) here: <p>Nikolnikov,S., Posfai,G. and Sain,B. <em>The construction of a versatile plasmid vector that allows direct<br> selection of fragments cloned into six unique sites of the cI gene of coliphage 434</em>. Gene 30 (1-3), 261-265 (1984)<P> References (unparsed) here: <p>Nikolnikov,S., Posfai,G. and Sain,B. <em>The construction of a versatile plasmid vector that allows direct<br> selection of fragments cloned into six unique sites of the cI gene of coliphage 434</em>. Gene 30 (1-3), 261-265 (1984)<P><P>
Bacteriophage 434
true
annotation1743
1
cI 434
range1743
1
1
669
annotation1745
1
LVA
range1745
1
631
669
annotation2213992
1
Help:Barcodes
range2213992
1
670
694
annotation7035
1
BBa_C0052
range7035
1
1
669
BBa_I0466
1
BBa_I0466
RhlR Protein Generator
mit
2004-07-12T11:00:00Z
2015-08-31T04:07:29Z
false
false
_6_
0
101
7
Released HQ 2013
In stock
false
RhlR can sense N-butyryl-HSL in the media and can activate transcription from R0071. It can be used as part of a receiver device.
true
component943330
1
BBa_B0012
component943304
1
BBa_B0034
component943320
1
BBa_B0010
component943314
1
BBa_C0071
annotation943330
1
BBa_B0012
range943330
1
902
942
annotation943314
1
BBa_C0071
range943314
1
19
780
annotation943320
1
BBa_B0010
range943320
1
814
893
annotation943304
1
BBa_B0034
range943304
1
1
12
BBa_P0153
1
BBa_P0153
PoPS -> cII (p22) [S0167]
Randy Rettberg
2004-04-24T11:00:00Z
2015-05-08T01:14:10Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Protein generator converting TIPS to the protein cII from p22. Used as the input section for Quad Part Inverters Q01530.
true
component944598
1
BBa_B0012
component944588
1
BBa_B0010
component944580
1
BBa_C0053
component944568
1
BBa_B0031
annotation944580
1
BBa_C0053
range944580
1
21
707
annotation944588
1
BBa_B0010
range944588
1
741
820
annotation944568
1
BBa_B0031
range944568
1
1
14
annotation944598
1
BBa_B0012
range944598
1
829
869
BBa_P0440
1
BBa_P0440
PoPS -> TetR [S0151]
Randy Rettberg
2004-04-26T11:00:00Z
2015-05-08T01:14:10Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
true
component944886
1
BBa_B0034
component944896
1
BBa_C0040
component944902
1
BBa_B0010
component944912
1
BBa_B0012
annotation944896
1
BBa_C0040
range944896
1
19
678
annotation944902
1
BBa_B0010
range944902
1
712
791
annotation944886
1
BBa_B0034
range944886
1
1
12
annotation944912
1
BBa_B0012
range944912
1
800
840
BBa_P0151
1
BBa_P0151
PoPS -> cI (lambda) [S0165]
Randy Rettberg
2004-04-24T11:00:00Z
2015-05-08T01:14:10Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Protein generator converting TIPS to the protein cI from lambda. Used as the input section for Quad Part Inverters Q01150 and Q01151.
true
component944519
1
BBa_B0010
component944529
1
BBa_B0012
component944513
1
BBa_C0051
component944503
1
BBa_B0031
annotation944503
1
BBa_B0031
range944503
1
1
14
annotation944513
1
BBa_C0051
range944513
1
21
770
annotation944519
1
BBa_B0010
range944519
1
804
883
annotation944529
1
BBa_B0012
range944529
1
892
932
BBa_R0040
1
p(tetR)
TetR repressible promoter
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Sequence for pTet inverting regulator driven by the TetR protein.</P>
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
true
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986787
1
-10
range1986787
1
43
48
annotation1986785
1
-35
range1986785
1
20
25
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_E0240
1
GFP report
GFP generator
Jennifer Braff
2004-10-17T11:00:00Z
2015-08-31T04:07:26Z
false
true
_11_1_
0
61
7
Released HQ 2013
In stock
false
B0032.E0040.B0015
true
component1249221
1
BBa_B0010
component1249216
1
BBa_E0040
component1249213
1
BBa_B0032
component1249231
1
BBa_B0012
annotation1249231
1
BBa_B0012
range1249231
1
836
876
annotation1249221
1
BBa_B0010
range1249221
1
748
827
annotation1249216
1
BBa_E0040
range1249216
1
20
739
annotation1249213
1
BBa_B0032
range1249213
1
1
13
BBa_I13507
1
BBa_I13507
Screening plasmid intermediate
jkm
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction.
true
component1524838
1
BBa_B0012
component1524815
1
BBa_B0034
component1524822
1
BBa_E1010
component1524828
1
BBa_B0010
annotation1524838
1
BBa_B0012
range1524838
1
821
861
annotation1524815
1
BBa_B0034
range1524815
1
1
12
annotation1524828
1
BBa_B0010
range1524828
1
733
812
annotation1524822
1
BBa_E1010
range1524822
1
19
699
BBa_I13500
1
BBa_I13500
Screening plasmid intermediate
jkm
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
false
true
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction
true
component1505002
1
BBa_E0040
component1505000
1
BBa_B0034
annotation1505002
1
BBa_E0040
range1505002
1
19
738
annotation1505000
1
BBa_B0034
range1505000
1
1
12
BBa_C0160
1
aiiA
autoinducer inactivation enzyme aiiA (no LVA)
jcbraff
2004-05-26T11:00:00Z
2015-08-31T04:07:24Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
same as C0060 except no LVA tag
true
annotation1891585
1
aiiA
range1891585
1
1
756
BBa_R0077
1
cinR
Promoter (cinR and HSL regulated, RBS+)
crackdots
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription.
This promoter contains the RBS: TGGAGG
The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci
Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ
MOL. MICROBIOLOGY 37(1): 81-97, 2000
Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
Rhizobium leguminosarum
true
annotation301205
1
stem_loop
range301205
1
91
136
annotation301204
1
stem_loop
range301204
1
35
74
annotation301150
1
embedded RBS
range301150
1
226
231
annotation301371
1
unidentified/uncharacterized CinR dependent
range301371
1
1
220
BBa_E0420
1
BBa_E0420
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
Caitlin Conboy
2004-03-15T12:00:00Z
2015-08-31T04:07:26Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Standard CFP output device w/o LVA tag.
true
component942787
1
BBa_B0010
component942782
1
BBa_E0020
component942780
1
BBa_B0034
component942797
1
BBa_B0012
annotation942787
1
BBa_B0010
range942787
1
750
829
annotation942780
1
BBa_B0034
range942780
1
1
12
annotation942782
1
BBa_E0020
range942782
1
19
741
annotation942797
1
BBa_B0012
range942797
1
838
878
BBa_R0074
1
PenI
Promoter (PenI regulated)
crackdots
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
extends slightly past -50
bacillus licheniformis
true
annotation319789
1
dimer right half
range319789
1
55
76
annotation319788
1
dimer left half
range319788
1
31
53
annotation319212
1
-10
range319212
1
46
51
annotation302713
1
PenI
range302713
1
1
77
annotation319211
1
-35
range319211
1
23
28
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
RBS based on Elowitz repressilator.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation23325
1
conserved
range23325
1
5
8
BBa_C0060
1
aiiA
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the autoinducer inactivation enzyme A (<em>aiiA</em>) LVA tagged. The gene was originally isolated from <em>Bacillus</em> sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.</P>
References (unparsed) here: <p>Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of <em>Erwinia<br> carotovora</em>, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). <a href="#">http://www.pnas.org/cgi/content/full/97/7/3526</a></P> <P>Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. <a href="#">http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491</a><br> <br> </P> <P> References (unparsed) here: <p>Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of <em>Erwinia<br> carotovora</em>, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). <a href="#">http://www.pnas.org/cgi/content/full/97/7/3526</a></P> <P>Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. <a href="#">http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491</a><br> <br> </P> <P>BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.<P>
<genbank>AF196486</genbank> from <em>Bacillus</em> sp. 240B1 putative metallohydrolase (<em>aiiA</em>) gene. <BR> Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of <em>Erwinia<br> carotovora</em>, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).<br>
true
annotation1755
1
2
range1755
1
784
789
annotation7037
1
BBa_C0060
range7037
1
1
789
annotation1754
1
start
range1754
1
1
3
annotation1756
1
LVA
range1756
1
751
783
annotation2213987
1
Help:Barcodes
range2213987
1
790
814
annotation1757
1
aiiA
range1757
1
1
750
BBa_C0261
1
BBa_C0261
AHL-making Enzyme, luxI (+RBS)
robertb
2004-08-22T11:00:00Z
2015-08-31T04:07:24Z
false
false
_6_
0
159
7
Released HQ 2013
In stock
true
B0034.C0061
true
component1060413
1
BBa_C0061
component1060403
1
BBa_B0034
annotation1060403
1
BBa_B0034
range1060403
1
1
12
annotation1060413
1
BBa_C0061
range1060413
1
19
636
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
jcbraff
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
false
true
_11_1_
4206
61
7
Released HQ 2013
In stock
false
GFP (mut3b) [note that this part does not have a barcode]
true
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
Randy Rettberg
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
In stock
false
Transcriptional terminator consisting of a 64 bp stem-loop.
true
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_I0462
1
LuxR
luxR Protein Generator
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-08-31T04:07:29Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Produces LuxR protein which can sense 3OC<sub>6</sub>HSL in the media and activate transcription from R0062, the right hand Lux promoter
true
component943189
1
BBa_B0010
component943166
1
BBa_B0034
component943181
1
BBa_C0062
component943199
1
BBa_B0012
annotation943189
1
BBa_B0010
range943189
1
808
887
annotation943181
1
BBa_C0062
range943181
1
19
774
annotation943199
1
BBa_B0012
range943199
1
896
936
annotation943166
1
BBa_B0034
range943166
1
1
12
BBa_B0040
1
spacer
Spacer.1 (generic)
Vinay S. Mahajan, Brian Chow, Peter Carr
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.</P>
<P> <P><p>The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the <a href="http://www.lifesci.ucsb.edu/~maduro/random.htm">Random DNA Generator </a>engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other <em>E.coli</em> sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. </p> <p> DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur):<br> T->A @ 85<br> T->A @ 42<br> C->T @ 79<br> A->T @ 64<br> A->T @ 31<br> T->A @ 34<br> C->A @ 37<br> Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences.<br> </p> <P>In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. </p><P> The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
Randomly generated and optimized for several parameters (see Design notes).
true
annotation1721
1
Spacer-1
range1721
1
1
70
annotation7030
1
BBa_B0040
range7030
1
1
70
BBa_R1062
1
lux pR
Promoter, Standard (luxR and HSL regulated -- lux pR)<br>
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr<br> Drew Endy<br>
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
[Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].</p> <p>Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"><br><br> Modified to comply with BBa_S0001:<br> TTGACA-17N-GATACT<br> <P>
<em>V. fischeri</em>
true
annotation2100
1
start
range2100
1
54
54
annotation7077
1
BBa_R1062
range7077
1
1
56
annotation2101
1
-35
range2101
1
20
25
annotation2099
1
-10
range2099
1
42
48
annotation2098
1
LuxR/HSL
range2098
1
1
20
BBa_R1051
1
cI lam
Promoter, Standard (lambda cI regulated)<br>
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross<br> Drew Endy<br>
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
[Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].<br><br>The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P>
<P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<br><br> Modified to comply with BBa_S0001:<br> TTGACA-17N-GATACT<br><P> Incompatible with host expressing cI repressor.
<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
true
annotation2075
1
-35
range2075
1
15
20
annotation7074
1
BBa_R1051
range7074
1
1
49
annotation2077
1
OR2
range2077
1
1
17
annotation2076
1
OR1
range2076
1
25
41
annotation2078
1
-10
range2078
1
38
43
BBa_I13003
1
BBa_I13003
medium RBS, YFP (-LVA), double terminator
Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
2004-06-27T11:00:00Z
2015-08-31T04:07:32Z
false
false
_6_
0
101
7
Released HQ 2013
In stock
false
-- No description --
true
component943495
1
BBa_B0012
component943477
1
BBa_B0032
component943485
1
BBa_B0010
component943480
1
BBa_E0030
annotation943480
1
BBa_E0030
range943480
1
20
742
annotation943485
1
BBa_B0010
range943485
1
751
830
annotation943477
1
BBa_B0032
range943477
1
1
13
annotation943495
1
BBa_B0012
range943495
1
839
879
BBa_R0065
1
cI+luxR
Promoter (lambda cI and luxR regulated -- hybrid)
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. </P> <P> </P> <table width="75%" border="1"> <tr> <td><strong>LuxI</strong></td> <td><strong>cI</strong></td> <td><strong>activity of promoter</strong></td> </tr> <tr> <td>+</td> <td>+</td> <td>zero</td> </tr> <tr> <td>+</td> <td>-</td> <td>maximum</td> </tr> <tr> <td>-</td> <td>+</td> <td>zero</td> </tr> <tr> <td>-</td> <td>-</td> <td>leaky (no quantitative information)</td> </tr> </table> <P> </P>
<P> <P>This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>. <P>
true
annotation1986776
1
-10
range1986776
1
47
52
annotation1986775
1
Lux Box
range1986775
1
6
25
annotation1986780
1
OR1 cI
range1986780
1
81
97
annotation1986781
1
-10
range1986781
1
94
97
annotation1986779
1
-35
range1986779
1
71
76
annotation1986778
1
lux p(R) start
range1986778
1
58
58
annotation1986777
1
OR2 cI
range1986777
1
57
73
annotation1986774
1
BBa_R0065
range1986774
1
1
97
BBa_R0082
1
OmpR
Promoter (OmpR, positive)
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1.
An alternate version, BBa_R0083, cuts out the C2 and C3 sites.
The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
NC_000193 E. coli K12
true
annotation301156
1
C3 OmpR
range301156
1
54
71
annotation301154
1
C1 OmpR
range301154
1
13
30
annotation301155
1
C2 OmpR
range301155
1
34
51
annotation301167
1
-10
range301167
1
98
103
annotation301166
1
-35
range301166
1
75
80
BBa_F2622
1
BBa_F2622
3OC<sub>6</sub>HSL Receiver Device
Barry Canton
2004-12-14T12:00:00Z
2015-08-31T04:07:27Z
false
true
_11_6_
0
135
7
Released HQ 2013
In stock
false
This device senses the 3OC<sub>6</sub>HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the lac repressible R0011.
true
component1484671
1
BBa_B0010
component1484696
1
BBa_R0062
component1484648
1
BBa_B0034
component1484681
1
BBa_B0012
component1484639
1
BBa_R0011
component1484663
1
BBa_C0062
annotation1484681
1
BBa_B0012
range1484681
1
959
999
annotation1484696
1
BBa_R0062
range1484696
1
1008
1062
annotation1484648
1
BBa_B0034
range1484648
1
64
75
annotation1484671
1
BBa_B0010
range1484671
1
871
950
annotation1484639
1
BBa_R0011
range1484639
1
1
54
annotation1484663
1
BBa_C0062
range1484663
1
82
837
BBa_I13600
1
BBa_I13600
Tet with CFP reporter (without LVA tag)
Christopher Batten, Victoria Chou, Kenneth Nesmith
2004-07-15T11:00:00Z
2015-08-31T04:07:35Z
false
true
_6_
0
101
7
Released HQ 2013
In stock
false
-- No description --
true
component947192
1
BBa_B0034
component947199
1
BBa_B0010
component947184
1
BBa_R0040
component947194
1
BBa_E0020
component947209
1
BBa_B0012
annotation947184
1
BBa_R0040
range947184
1
1
54
annotation947194
1
BBa_E0020
range947194
1
81
803
annotation947209
1
BBa_B0012
range947209
1
900
940
annotation947199
1
BBa_B0010
range947199
1
812
891
annotation947192
1
BBa_B0034
range947192
1
63
74
BBa_K125500
1
BBa_K125500
GFP fusion brick
Grace Kwan
2008-07-24T11:00:00Z
2016-01-25T02:34:56Z
false
false
_179_
4206
3229
9
In stock
false
The first two nucleotides of the GFP start codon were removed to create this fusion brick.
The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
false
annotation1968260
1
GFP fusion brick
range1968260
1
1
718
annotation1969908
1
deletion of TG
range1969908
1
1
1
BBa_E0036
1
EYFP
enhanced yellow fluorescent protein derived from A. victoria GFP
jcbraff
2004-06-06T11:00:00Z
2015-08-31T04:07:25Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
-- No description --
true
BBa_R0053
1
cII p22
Promoter (p22 cII regulated)
Maia Mahoney
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it.<br> This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)</p>
<P> <P><P>
Bacteriophage p22.
true
annotation2038
1
-35
range2038
1
18
23
annotation2035
1
OR3
range2035
1
1
3
annotation7069
1
BBa_R0053
range7069
1
1
54
annotation2042
1
-10
range2042
1
30
35
annotation2037
1
OR1
range2037
1
34
51
annotation2041
1
-35
range2041
1
8
13
annotation2036
1
OR2
range2036
1
11
28
BBa_E0032
1
YFP
enhanced yellow fluorescent protein derived from A. victoria GFP
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
2003-01-31T12:00:00Z
2015-08-31T04:07:25Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. </P> Annotated mutation cause a Q81L mutation that appears to not be near the active site. </P>
<P> <P>BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
Modified from <bb_part>BBa_E0031</bb_part>
true
annotation2159
1
2
range2159
1
757
762
annotation2156
1
YFP (LVA)
range2156
1
1
762
annotation7041
1
BBa_E0032
range7041
1
1
762
annotation2160
1
SsrA
range2160
1
718
756
annotation2161
1
A (Q->L)
range2161
1
242
242
BBa_I13001
1
BBa_I13001
RBS B0030 w/ YFP (-LVA) TT
Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
2004-06-27T11:00:00Z
2015-08-31T04:07:32Z
false
false
_6_
0
101
7
Released HQ 2013
In stock
false
B0030. E0030. B0015
true
component943431
1
BBa_E0030
component943446
1
BBa_B0012
component943436
1
BBa_B0010
component943428
1
BBa_B0030
annotation943428
1
BBa_B0030
range943428
1
1
15
annotation943446
1
BBa_B0012
range943446
1
841
881
annotation943436
1
BBa_B0010
range943436
1
753
832
annotation943431
1
BBa_E0030
range943431
1
22
744
BBa_J23119
1
BBa_J23119
constitutive promoter family member
John Anderson
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
false
Later
N/A
Overlap extension of synthetic oligonucleotides
true
BBa_E0034
1
EYFP
enhanced yellow fluorescent protein derived from A. victoria GFP
jcbraff
2004-06-06T11:00:00Z
2015-08-31T04:07:25Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
-- No description --
true
annotation1245671
1
stop
range1245671
1
757
762
annotation1245672
1
AAV
range1245672
1
718
756
BBa_E0840
1
GFP genera
GFP generator
Jennifer Braff
2004-10-17T11:00:00Z
2015-08-31T04:07:26Z
false
true
_11_1_
0
61
7
Released HQ 2013
In stock
true
B0030.E0040.B0015
true
component1249242
1
BBa_E0040
component1249257
1
BBa_B0012
component1249247
1
BBa_B0010
component1249239
1
BBa_B0030
annotation1249257
1
BBa_B0012
range1249257
1
838
878
annotation1249247
1
BBa_B0010
range1249247
1
750
829
annotation1249239
1
BBa_B0030
range1249239
1
1
15
annotation1249242
1
BBa_E0040
range1249242
1
22
741
BBa_I15016
1
ecfp
B0032.ECFP
Jeff Tabor
2005-04-14T11:00:00Z
2015-08-31T04:07:38Z
false
false
_5_
0
88
5
Released HQ 2013
In stock
false
This part accepts POPS to produce ECFP under the medium RBS B0032
true
component1478405
1
BBa_B0032
component1478408
1
BBa_E0020
annotation1478408
1
BBa_E0020
range1478408
1
20
742
annotation1478405
1
BBa_B0032
range1478405
1
1
13
BBa_S03154
1
BBa_S03154
B0034.C0078
Jennifer Braff
2004-07-22T11:00:00Z
2015-05-08T01:14:20Z
false
false
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
true
component940972
1
BBa_C0078
component940962
1
BBa_B0034
annotation940962
1
BBa_B0034
range940962
1
1
12
annotation940972
1
BBa_C0078
range940972
1
19
660
BBa_R0078
1
cinR
Promoter (cinR and HSL regulated)
Drew Endy
2004-01-29T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Rhizobium leguminosarum
true
annotation318234
1
unidentified/uncharacterized CinR dependent
range318234
1
1
220
annotation318244
1
stem_loop
range318244
1
35
74
annotation318250
1
stem_loop
range318250
1
91
136
BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation1714
1
RBS
range1714
1
7
10
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
annotation7028
1
BBa_B0033
range7028
1
1
11
BBa_I13602
1
BBa_I13602
Tet operator with CFP reporter (with LVA tag) [R/Tc+]
Christopher Batten, Victoria Chou, Kenneth Nesmith
2004-07-15T11:00:00Z
2015-08-31T04:07:36Z
false
true
_6_
0
101
7
Released HQ 2013
In stock
false
-- No description --
true
component947282
1
BBa_B0010
component947262
1
BBa_B0034
component947292
1
BBa_B0012
component947254
1
BBa_R0040
component947274
1
BBa_E0022
annotation947254
1
BBa_R0040
range947254
1
1
54
annotation947262
1
BBa_B0034
range947262
1
63
74
annotation947274
1
BBa_E0022
range947274
1
81
842
annotation947292
1
BBa_B0012
range947292
1
939
979
annotation947282
1
BBa_B0010
range947282
1
851
930