iGEM_2019_Plasmid_pSB1AK3
1
iGEM 2019 Plasmid pSB1AK3
BBa_J04450
1
BBa_J04450
RFP Coding Device
Tamar Odle
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
false
true
_16_
0
328
16
Released HQ 2013
In stock
false
Contains an IPTG inducible promoter an RBS, RFP, and terminator.
Davidson Synth-Aces
true
component1509404
1
BBa_B0034
component1509411
1
BBa_E1010
component1509427
1
BBa_B0012
component1509417
1
BBa_B0010
component1509394
1
BBa_R0010
annotation1509411
1
BBa_E1010
range1509411
1
227
907
annotation1509427
1
BBa_B0012
range1509427
1
1029
1069
annotation1509394
1
BBa_R0010
range1509394
1
1
200
annotation1509404
1
BBa_B0034
range1509404
1
209
220
annotation1509417
1
BBa_B0010
range1509417
1
941
1020
BBa_J52026
1
BBa_J52026
dnMyD88-linker-GFP
Jelka Pohar
2006-10-14T11:00:00Z
2016-01-26T09:39:38Z
false
false
_80_
4206
855
80
In stock
false
dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor). dnMyD88 is linked to GFP (acts as a reporter) through six aminoacid long protein linker.
dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence.
dnMyD88-
GFP-
more to come
true
annotation1903011
1
dnMyD88-linker-GFP
range1903011
1
1
1155
annotation1903008
1
dnMyD88
range1903008
1
1
417
annotation1903009
1
linker
range1903009
1
418
435
annotation1903010
1
GFP
range1903010
1
436
1155