iGEM 2019 Plasmid pSB1C6
type: Collection
persistent identity: iGEM 2019 Plasmid pSB1C6
display id: iGEM_2019_Plasmid_pSB1C6
version: 1
owned by: myers
member: BBa_K1114100
member: BBa_K1114014
member: BBa_K1484103
member: BBa_K1114007
member: BBa_K1114005
member: BBa_K1114104
member: BBa_K1114000
member: BBa_K1401008
member: BBa_K1114107
member: BBa_K1114021
member: BBa_K783028
member: BBa_K1114103
member: BBa_K783032
member: BBa_K1114025
member: BBa_K783024
member: BBa_K1742004
member: BBa_K1114012
member: BBa_K1114111
member: BBa_K1114003
member: BBa_K1114102
member: BBa_K1114009
member: BBa_K1114016
member: BBa_K1114108
member: BBa_K1114020
member: BBa_K1114018
member: BBa_K783031
member: BBa_K1114011
member: BBa_K1114110
member: BBa_K1114010
member: BBa_K1114002
member: BBa_K1114023
member: BBa_K1114101
member: BBa_K1742002
member: BBa_K1114109

BBa_K1484103
type: ComponentDefinition
persistent identity: BBa K1484103
display id: BBa_K1484103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Benzalacetone Synthase, compatible with MoClo
creator: Guy Lewy
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1864_
m user id: 0
owner id: 21057
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Synthesis of BA
mutable notes: Restriction sites
mutable provenance: Raspberry genome, lab in Kyoto
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MoClo fusion site
sequence annotation: Benzalacetone Synthase
sequence: https://synbiohub.org/public/igem/BBa_K1484103_sequence/1

MoClo fusion site
type: SequenceAnnotation
persistent identity: annotation2416924
display id: annotation2416924
version: 1
top level: BBa_K1484103
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484103/annotation2416924/range2416924/1

https://synbiohub.org/public/igem/BBa_K1484103/annotation2416924/range2416924/1
type: Range
persistent identity: range2416924
display id: range2416924
version: 1
direction: direction/forward
top level: BBa_K1484103
start: 1164
end: 1174
orientation: inline

MoClo fusion site
type: SequenceAnnotation
persistent identity: annotation2416923
display id: annotation2416923
version: 1
top level: BBa_K1484103
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484103/annotation2416923/range2416923/1

https://synbiohub.org/public/igem/BBa_K1484103/annotation2416923/range2416923/1
type: Range
persistent identity: range2416923
display id: range2416923
version: 1
direction: direction/forward
top level: BBa_K1484103
start: 1
end: 11
orientation: inline

Benzalacetone Synthase
type: SequenceAnnotation
persistent identity: annotation2416922
display id: annotation2416922
version: 1
top level: BBa_K1484103
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1484103/annotation2416922/range2416922/1

https://synbiohub.org/public/igem/BBa_K1484103/annotation2416922/range2416922/1
type: Range
persistent identity: range2416922
display id: range2416922
version: 1
direction: direction/forward
top level: BBa_K1484103
start: 9
end: 1163
orientation: inline

BBa_K1114025
type: ComponentDefinition
persistent identity: BBa K1114025
display id: BBa_K1114025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_R0040 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_R0040 with AB fusion sites. See the BBa_R0040 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_R0040 with AB fusion sites. See the BBa_R0040 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114025_sequence/1

BBa_K1114002
type: ComponentDefinition
persistent identity: BBa K1114002
display id: BBa_K1114002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23102 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23102 with EB fusion sites. See the BBa_J23102 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of BBa_J23102 with EB fusion sites. See the BBa_J23102 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114002_sequence/1

BBa_K1114014
type: ComponentDefinition
persistent identity: BBa K1114014
display id: BBa_K1114014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23112 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: __NOTOC__ <partinfo>BBa_K1114014 short</partinfo> <partinfo>BBa_K1114014 SequenceAndFeatures</partinfo> ===Design Notes=== This is the MoClo formatted part of BBa_J23112 with AB fusion sites. See the BBa_J23112 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: __NOTOC__ <partinfo>BBa_K1114014 short</partinfo> <partinfo>BBa_K1114014 SequenceAndFeatures</partinfo> ===Design Notes=== This is the MoClo formatted part of BBa_J23112 with AB fusion sites. See the BBa_J23112 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114014_sequence/1

BBa_K1114016
type: ComponentDefinition
persistent identity: BBa K1114016
display id: BBa_K1114016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23113 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_J23113 with AB fusion sites. See the BBa_J23113 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_J23113 with AB fusion sites. See the BBa_J23113 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114016_sequence/1

BBa_K1114009
type: ComponentDefinition
persistent identity: BBa K1114009
display id: BBa_K1114009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23106 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23106 with AB fusion sites. See the BBa_J23106 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable notes: This is the MoClo formatted part of BBa_J23106 with AB fusion sites. See the BBa_J23106 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114009_sequence/1

BBa_K1114005
type: ComponentDefinition
persistent identity: BBa K1114005
display id: BBa_K1114005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23104 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_J23104
sequence annotation: MoClo Fusion Site B
sequence annotation: MoClo Fusion Site A
sequence: https://synbiohub.org/public/igem/BBa_K1114005_sequence/1

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2361748
display id: annotation2361748
version: 1
top level: BBa_K1114005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1114005/annotation2361748/range2361748/1

https://synbiohub.org/public/igem/BBa_K1114005/annotation2361748/range2361748/1
type: Range
persistent identity: range2361748
display id: range2361748
version: 1
direction: direction/forward
top level: BBa_K1114005
start: 5
end: 39
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361747
display id: annotation2361747
version: 1
top level: BBa_K1114005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114005/annotation2361747/range2361747/1

https://synbiohub.org/public/igem/BBa_K1114005/annotation2361747/range2361747/1
type: Range
persistent identity: range2361747
display id: range2361747
version: 1
direction: direction/forward
top level: BBa_K1114005
start: 40
end: 43
orientation: inline

MoClo Fusion Site A
type: SequenceAnnotation
persistent identity: annotation2361746
display id: annotation2361746
version: 1
top level: BBa_K1114005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114005/annotation2361746/range2361746/1

https://synbiohub.org/public/igem/BBa_K1114005/annotation2361746/range2361746/1
type: Range
persistent identity: range2361746
display id: range2361746
version: 1
direction: direction/forward
top level: BBa_K1114005
start: 1
end: 4
orientation: inline

BBa_K1114110
type: ComponentDefinition
persistent identity: BBa K1114110
display id: BBa_K1114110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic design RBS 13, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 13 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 13 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BCD13
sequence annotation: MoClo Fusion Site C
sequence annotation: MoClo Fusion Site B
sequence: https://synbiohub.org/public/igem/BBa_K1114110_sequence/1

BCD13
type: SequenceAnnotation
persistent identity: annotation2361884
display id: annotation2361884
version: 1
top level: BBa_K1114110
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114110/annotation2361884/range2361884/1

https://synbiohub.org/public/igem/BBa_K1114110/annotation2361884/range2361884/1
type: Range
persistent identity: range2361884
display id: range2361884
version: 1
direction: direction/forward
top level: BBa_K1114110
start: 5
end: 88
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361883
display id: annotation2361883
version: 1
top level: BBa_K1114110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114110/annotation2361883/range2361883/1

https://synbiohub.org/public/igem/BBa_K1114110/annotation2361883/range2361883/1
type: Range
persistent identity: range2361883
display id: range2361883
version: 1
direction: direction/forward
top level: BBa_K1114110
start: 89
end: 92
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361882
display id: annotation2361882
version: 1
top level: BBa_K1114110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114110/annotation2361882/range2361882/1

https://synbiohub.org/public/igem/BBa_K1114110/annotation2361882/range2361882/1
type: Range
persistent identity: range2361882
display id: range2361882
version: 1
direction: direction/forward
top level: BBa_K1114110
start: 1
end: 4
orientation: inline

BBa_K1401008
type: ComponentDefinition
persistent identity: BBa K1401008
display id: BBa_K1401008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tandem Promoter pBad-pTet
creator: Kathleen Lewis
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1779_
m user id: 0
owner id: 22443
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. The promoter is induced by atc and arabinose. The characterization data will be updated before the jamboree.
mutable notes: We looked through the literature and decided to attach the two promoters without any extra sequence between them
mutable provenance: This was formed using BBa_I13453 and BBa_R0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1401008_sequence/1

BBa_K783032
type: ComponentDefinition
persistent identity: BBa K783032
display id: BBa_K783032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_J23115
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_J23115. It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_J23115
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_J23115
sequence: https://synbiohub.org/public/igem/BBa_K783032_sequence/1

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2214233
display id: annotation2214233
version: 1
top level: BBa_K783032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K783032/annotation2214233/range2214233/1

https://synbiohub.org/public/igem/BBa_K783032/annotation2214233/range2214233/1
type: Range
persistent identity: range2214233
display id: range2214233
version: 1
direction: direction/reverse
top level: BBa_K783032
start: 1
end: 35
orientation: inline

BBa_K1114000
type: ComponentDefinition
persistent identity: BBa K1114000
display id: BBa_K1114000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23100 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part.
mutable notes: This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part. Summary of Modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114000_sequence/1

BBa_K1114018
type: ComponentDefinition
persistent identity: BBa K1114018
display id: BBa_K1114018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23116 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_J23116 with AB fusion sites. See the BBa_J23116 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_J23116 with AB fusion sites. See the BBa_J23116 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114018_sequence/1

BBa_K1114109
type: ComponentDefinition
persistent identity: BBa K1114109
display id: BBa_K1114109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic design RBS 12, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: MoClo Fusion Site C
sequence annotation: MoClo Fusion Site B
sequence annotation: BCD12
sequence: https://synbiohub.org/public/igem/BBa_K1114109_sequence/1

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361877
display id: annotation2361877
version: 1
top level: BBa_K1114109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114109/annotation2361877/range2361877/1

https://synbiohub.org/public/igem/BBa_K1114109/annotation2361877/range2361877/1
type: Range
persistent identity: range2361877
display id: range2361877
version: 1
direction: direction/forward
top level: BBa_K1114109
start: 89
end: 92
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361876
display id: annotation2361876
version: 1
top level: BBa_K1114109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114109/annotation2361876/range2361876/1

https://synbiohub.org/public/igem/BBa_K1114109/annotation2361876/range2361876/1
type: Range
persistent identity: range2361876
display id: range2361876
version: 1
direction: direction/forward
top level: BBa_K1114109
start: 1
end: 4
orientation: inline

BCD12
type: SequenceAnnotation
persistent identity: annotation2361880
display id: annotation2361880
version: 1
top level: BBa_K1114109
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114109/annotation2361880/range2361880/1

https://synbiohub.org/public/igem/BBa_K1114109/annotation2361880/range2361880/1
type: Range
persistent identity: range2361880
display id: range2361880
version: 1
direction: direction/forward
top level: BBa_K1114109
start: 5
end: 88
orientation: inline

BBa_K1114103
type: ComponentDefinition
persistent identity: BBa K1114103
display id: BBa_K1114103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo formatted version of B0033 with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_B0033 with BC fusion sites. See the BBa_B0033 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of BBa_B0033 with BC fusion sites. See the BBa_B0033 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114103_sequence/1

BBa_K1114111
type: ComponentDefinition
persistent identity: BBa K1114111
display id: BBa_K1114111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic design RBS 16, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 16 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 16 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BCD16
sequence annotation: MoClo Fusion Site B
sequence annotation: MoClo Fusion Site C
sequence: https://synbiohub.org/public/igem/BBa_K1114111_sequence/1

BCD16
type: SequenceAnnotation
persistent identity: annotation2361887
display id: annotation2361887
version: 1
top level: BBa_K1114111
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114111/annotation2361887/range2361887/1

https://synbiohub.org/public/igem/BBa_K1114111/annotation2361887/range2361887/1
type: Range
persistent identity: range2361887
display id: range2361887
version: 1
direction: direction/forward
top level: BBa_K1114111
start: 5
end: 88
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361885
display id: annotation2361885
version: 1
top level: BBa_K1114111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114111/annotation2361885/range2361885/1

https://synbiohub.org/public/igem/BBa_K1114111/annotation2361885/range2361885/1
type: Range
persistent identity: range2361885
display id: range2361885
version: 1
direction: direction/forward
top level: BBa_K1114111
start: 1
end: 4
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361886
display id: annotation2361886
version: 1
top level: BBa_K1114111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114111/annotation2361886/range2361886/1

https://synbiohub.org/public/igem/BBa_K1114111/annotation2361886/range2361886/1
type: Range
persistent identity: range2361886
display id: range2361886
version: 1
direction: direction/forward
top level: BBa_K1114111
start: 89
end: 92
orientation: inline

BBa_K783028
type: ComponentDefinition
persistent identity: BBa K783028
display id: BBa_K783028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_J23110
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_J23110. It has two MoClo fusion sites flanking this part.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_J23110
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K783028_sequence/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2214228
display id: annotation2214228
version: 1
top level: BBa_K783028
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K783028/annotation2214228/range2214228/1

https://synbiohub.org/public/igem/BBa_K783028/annotation2214228/range2214228/1
type: Range
persistent identity: range2214228
display id: range2214228
version: 1
direction: direction/reverse
top level: BBa_K783028
start: 1
end: 35
orientation: inline

BBa_K1114101
type: ComponentDefinition
persistent identity: BBa K1114101
display id: BBa_K1114101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo format of a modified version of BBa_B0031 with BC fusion sites.
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a modified BBa_B0031 with BC fusion sites. See the BBa_B0031 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of a modified BBa_B0031 with BC fusion sites. See the BBa_B0031 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114101_sequence/1

BBa_K1114100
type: ComponentDefinition
persistent identity: BBa K1114100
display id: BBa_K1114100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo format of a modified version of BBa_B0030 with BC fusion sites.
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a modified BBa_B0030 with BC fusion sites. See the BBa_R0010 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_B0030???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of a modified BBa_B0030 with BC fusion sites. See the BBa_R0010 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_B0030???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114100_sequence/1

BBa_K1114021
type: ComponentDefinition
persistent identity: BBa K1114021
display id: BBa_K1114021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23118 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_J23118 with AB fusion sites. See the BBa_J23118 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_J23118 with AB fusion sites. See the BBa_J23118 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1114021_sequence/1

BBa_K1114012
type: ComponentDefinition
persistent identity: BBa K1114012
display id: BBa_K1114012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23109 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: __NOTOC__ <partinfo>BBa_K1114012 short</partinfo> <partinfo>BBa_K1114012 SequenceAndFeatures</partinfo> ===Design Notes=== This is the MoClo formatted part of BBa_J23109 with AB fusion sites. See the BBa_J23109 page for full information on the part. This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. ===Source=== iGEM Distribution Kit ===References===
mutable notes: __NOTOC__ <partinfo>BBa_K1114033 short</partinfo> <partinfo>BBa_K1114033 SequenceAndFeatures</partinfo> ===Design Notes=== This is the MoClo formatted part of BBa_J23109 with FB fusion sites. See the BBa_J23109 page for full information on the part. This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114012_sequence/1

BBa_K1114020
type: ComponentDefinition
persistent identity: BBa K1114020
display id: BBa_K1114020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23117 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_J23117 with AB fusion sites. See the BBa_J23117 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_J23117 with AB fusion sites. See the BBa_J23117 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114020_sequence/1

BBa_K1114108
type: ComponentDefinition
persistent identity: BBa K1114108
display id: BBa_K1114108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic design RBS 8, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: MoClo Fusion Site B
sequence annotation: BCD8
sequence annotation: MoClo Fusion Site C
sequence: https://synbiohub.org/public/igem/BBa_K1114108_sequence/1

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361725
display id: annotation2361725
version: 1
top level: BBa_K1114108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114108/annotation2361725/range2361725/1

https://synbiohub.org/public/igem/BBa_K1114108/annotation2361725/range2361725/1
type: Range
persistent identity: range2361725
display id: range2361725
version: 1
direction: direction/forward
top level: BBa_K1114108
start: 1
end: 4
orientation: inline

BCD8
type: SequenceAnnotation
persistent identity: annotation2361723
display id: annotation2361723
version: 1
top level: BBa_K1114108
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114108/annotation2361723/range2361723/1

https://synbiohub.org/public/igem/BBa_K1114108/annotation2361723/range2361723/1
type: Range
persistent identity: range2361723
display id: range2361723
version: 1
direction: direction/forward
top level: BBa_K1114108
start: 5
end: 88
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361726
display id: annotation2361726
version: 1
top level: BBa_K1114108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114108/annotation2361726/range2361726/1

https://synbiohub.org/public/igem/BBa_K1114108/annotation2361726/range2361726/1
type: Range
persistent identity: range2361726
display id: range2361726
version: 1
direction: direction/forward
top level: BBa_K1114108
start: 89
end: 92
orientation: inline

BBa_K1114010
type: ComponentDefinition
persistent identity: BBa K1114010
display id: BBa_K1114010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23107 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23107 with AB fusion sites. See the BBa_J23107 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable notes: This is the MoClo formatted part of BBa_J23107 with AB fusion sites. See the BBa_J23107 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114010_sequence/1

BBa_K1742002
type: ComponentDefinition
persistent identity: BBa K1742002
display id: BBa_K1742002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145N
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:27:00Z
discontinued: false
dominant: false
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn.
mutable notes: It is codon optimized for plants
mutable provenance: Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742002_sequence/1

BBa_K1114023
type: ComponentDefinition
persistent identity: BBa K1114023
display id: BBa_K1114023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_I13453 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_I13453 with AB fusion sites. See the BBa_I13453 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_I13453 with AB fusion sites. See the BBa_I13453 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114023_sequence/1

BBa_K1114107
type: ComponentDefinition
persistent identity: BBa K1114107
display id: BBa_K1114107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic Design RBS 2, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: MoClo Fusion Site B
sequence annotation: BCD2
sequence annotation: MoClo Fusion Site C
sequence: https://synbiohub.org/public/igem/BBa_K1114107_sequence/1

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361727
display id: annotation2361727
version: 1
top level: BBa_K1114107
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361727/range2361727/1

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361727/range2361727/1
type: Range
persistent identity: range2361727
display id: range2361727
version: 1
direction: direction/forward
top level: BBa_K1114107
start: 1
end: 4
orientation: inline

BCD2
type: SequenceAnnotation
persistent identity: annotation2361724
display id: annotation2361724
version: 1
top level: BBa_K1114107
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361724/range2361724/1

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361724/range2361724/1
type: Range
persistent identity: range2361724
display id: range2361724
version: 1
direction: direction/forward
top level: BBa_K1114107
start: 5
end: 88
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361728
display id: annotation2361728
version: 1
top level: BBa_K1114107
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361728/range2361728/1

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361728/range2361728/1
type: Range
persistent identity: range2361728
display id: range2361728
version: 1
direction: direction/forward
top level: BBa_K1114107
start: 89
end: 92
orientation: inline

BBa_K783031
type: ComponentDefinition
persistent identity: BBa K783031
display id: BBa_K783031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_J23114
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_J23114. It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_J23114
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K783031_sequence/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2214229
display id: annotation2214229
version: 1
top level: BBa_K783031
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K783031/annotation2214229/range2214229/1

https://synbiohub.org/public/igem/BBa_K783031/annotation2214229/range2214229/1
type: Range
persistent identity: range2214229
display id: range2214229
version: 1
direction: direction/reverse
top level: BBa_K783031
start: 1
end: 35
orientation: inline

BBa_K783024
type: ComponentDefinition
persistent identity: BBa K783024
display id: BBa_K783024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_R0063
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo converted version of BBa_R0063, which is the negatively regulated pLuxR promoter. It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_R0063
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: Putative LuxR/HSL
sequence annotation: -10
sequence annotation: BBa_R0063
sequence: https://synbiohub.org/public/igem/BBa_K783024_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2214226
display id: annotation2214226
version: 1
top level: BBa_K783024
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214226/range2214226/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214226/range2214226/1
type: Range
persistent identity: range2214226
display id: range2214226
version: 1
direction: direction/forward
top level: BBa_K783024
start: 128
end: 128
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2214222
display id: annotation2214222
version: 1
top level: BBa_K783024
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214222/range2214222/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214222/range2214222/1
type: Range
persistent identity: range2214222
display id: range2214222
version: 1
direction: direction/forward
top level: BBa_K783024
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2214224
display id: annotation2214224
version: 1
top level: BBa_K783024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214224/range2214224/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214224/range2214224/1
type: Range
persistent identity: range2214224
display id: range2214224
version: 1
direction: direction/forward
top level: BBa_K783024
start: 89
end: 94
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2214227
display id: annotation2214227
version: 1
top level: BBa_K783024
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214227/range2214227/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214227/range2214227/1
type: Range
persistent identity: range2214227
display id: range2214227
version: 1
direction: direction/forward
top level: BBa_K783024
start: 130
end: 149
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2214225
display id: annotation2214225
version: 1
top level: BBa_K783024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214225/range2214225/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214225/range2214225/1
type: Range
persistent identity: range2214225
display id: range2214225
version: 1
direction: direction/forward
top level: BBa_K783024
start: 115
end: 122
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2214223
display id: annotation2214223
version: 1
top level: BBa_K783024
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214223/range2214223/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214223/range2214223/1
type: Range
persistent identity: range2214223
display id: range2214223
version: 1
direction: direction/forward
top level: BBa_K783024
start: 1
end: 151
orientation: inline

BBa_K1114011
type: ComponentDefinition
persistent identity: BBa K1114011
display id: BBa_K1114011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23108 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: <partinfo> BBa_K1114011 </partinfo> <partinfo> BBa_K1114011 SequenceAndFeatures </partinfo> This is the MoClo formatted part of BBa_J23108 with AB fusion sites. See the BBa_J23108 page for full information on the part. This is a MoClo level 0 promoter with a flanking site A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: <partinfo> BBa_K1114011 </partinfo> <partinfo> BBa_K1114011 SequenceAndFeatures </partinfo> This is the MoClo formatted part of BBa_J23108 with AB fusion sites. See the BBa_J23108 page for full information on the part. This is a MoClo level 0 promoter with a flanking site A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114011_sequence/1

BBa_K1742004
type: ComponentDefinition
persistent identity: BBa K1742004
display id: BBa_K1742004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhiC31 Plant codon optimized
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2015-12-16T01:52:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: It is a CDS of PhiC31 integrase from Streptomyces phage. It has a nuclear location sequence in the N-terminus.
mutable notes: It has a nuclear location sequence (NLS) and its codons are optimized for plants.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742004_sequence/1

BBa_K1114104
type: ComponentDefinition
persistent identity: BBa K1114104
display id: BBa_K1114104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo formatted version of B0034 with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_B0034 with BC fusion sites. See the BBa_B0034 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of BBa_B0034 with BC fusion sites. See the BBa_B0034 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114104_sequence/1

BBa_K1114003
type: ComponentDefinition
persistent identity: BBa K1114003
display id: BBa_K1114003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23103 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114003_sequence/1

BBa_K1114102
type: ComponentDefinition
persistent identity: BBa K1114102
display id: BBa_K1114102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo format of a modified version of BBa_B0032 with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a modified BBa_B0032 with BC fusion sites. See the BBa_B0032 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of a modified BBa_B0032 with BC fusion sites. See the BBa_B0032 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114102_sequence/1

BBa_K1114007
type: ComponentDefinition
persistent identity: BBa K1114007
display id: BBa_K1114007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23105 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23105 with AB fusion sites. See the BBa_J23105 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable notes: This is the MoClo formatted part of BBa_J23105 with AB fusion sites. See the BBa_J23105 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114007_sequence/1