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version: 1
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member: BBa_K608006

iGEM 2019 Distribution Plate 1 Well 18F
type: Collection
persistent identity: iGEM 2019 Plate1 Well18F
display id: iGEM_2019_Plate1_Well18F
version: 1
owned by: myers
member: BBa_K575044

iGEM 2019 Distribution Plate 1 Well 7N
type: Collection
persistent identity: iGEM 2019 Plate1 Well7N
display id: iGEM_2019_Plate1_Well7N
version: 1
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member: BBa_K733017

iGEM 2019 Distribution Plate 1 Well 20D
type: Collection
persistent identity: iGEM 2019 Plate1 Well20D
display id: iGEM_2019_Plate1_Well20D
version: 1
owned by: myers
member: 9A1

iGEM 2019 Distribution Plate 1 Well 23E
type: Collection
persistent identity: iGEM 2019 Plate1 Well23E
display id: iGEM_2019_Plate1_Well23E
version: 1
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member: BBa_K656010

iGEM 2019 Distribution Plate 1 Well 21H
type: Collection
persistent identity: iGEM 2019 Plate1 Well21H
display id: iGEM_2019_Plate1_Well21H
version: 1
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member: ArgBox8

iGEM 2019 Distribution Plate 1 Well 6C
type: Collection
persistent identity: iGEM 2019 Plate1 Well6C
display id: iGEM_2019_Plate1_Well6C
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member: BBa_K575013

iGEM 2019 Distribution Plate 1 Well 11J
type: Collection
persistent identity: iGEM 2019 Plate1 Well11J
display id: iGEM_2019_Plate1_Well11J
version: 1
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member: BBa_K738002

iGEM 2019 Distribution Plate 1 Well 4H
type: Collection
persistent identity: iGEM 2019 Plate1 Well4H
display id: iGEM_2019_Plate1_Well4H
version: 1
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member: MpAAT1

iGEM 2019 Distribution Plate 1 Well 1H
type: Collection
persistent identity: iGEM 2019 Plate1 Well1H
display id: iGEM_2019_Plate1_Well1H
version: 1
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member: tphC_322aa

iGEM 2019 Distribution Plate 1 Well 20M
type: Collection
persistent identity: iGEM 2019 Plate1 Well20M
display id: iGEM_2019_Plate1_Well20M
version: 1
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member: BBa_K823006

iGEM 2019 Distribution Plate 1 Well 19K
type: Collection
persistent identity: iGEM 2019 Plate1 Well19K
display id: iGEM_2019_Plate1_Well19K
version: 1
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member: YF1

iGEM 2019 Distribution Plate 1 Well 24C
type: Collection
persistent identity: iGEM 2019 Plate1 Well24C
display id: iGEM_2019_Plate1_Well24C
version: 1
owned by: myers
member: otsB

iGEM 2019 Distribution Plate 1 Well 23F
type: Collection
persistent identity: iGEM 2019 Plate1 Well23F
display id: iGEM_2019_Plate1_Well23F
version: 1
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member: BBa_K813001

iGEM 2019 Distribution Plate 1 Well 10H
type: Collection
persistent identity: iGEM 2019 Plate1 Well10H
display id: iGEM_2019_Plate1_Well10H
version: 1
owned by: myers
member: BBa_K606017

iGEM 2019 Distribution Plate 1 Well 2B
type: Collection
persistent identity: iGEM 2019 Plate1 Well2B
display id: iGEM_2019_Plate1_Well2B
version: 1
owned by: myers
member: BBa_K325210

iGEM 2019 Distribution Plate 1 Well 23G
type: Collection
persistent identity: iGEM 2019 Plate1 Well23G
display id: iGEM_2019_Plate1_Well23G
version: 1
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member: BBa_K577006

iGEM 2019 Distribution Plate 1 Well 8G
type: Collection
persistent identity: iGEM 2019 Plate1 Well8G
display id: iGEM_2019_Plate1_Well8G
version: 1
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member: BBa_K611025

iGEM 2019 Distribution Plate 1 Well 18H
type: Collection
persistent identity: iGEM 2019 Plate1 Well18H
display id: iGEM_2019_Plate1_Well18H
version: 1
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member: BBa_K575045

iGEM 2019 Distribution Plate 1 Well 19I
type: Collection
persistent identity: iGEM 2019 Plate1 Well19I
display id: iGEM_2019_Plate1_Well19I
version: 1
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member: BFP

iGEM 2019 Distribution Plate 1 Well 7L
type: Collection
persistent identity: iGEM 2019 Plate1 Well7L
display id: iGEM_2019_Plate1_Well7L
version: 1
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member: BBa_K733016

iGEM 2019 Distribution Plate 1 Well 16P
type: Collection
persistent identity: iGEM 2019 Plate1 Well16P
display id: iGEM_2019_Plate1_Well16P
version: 1
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member: BBa_K575038

iGEM 2019 Distribution Plate 1 Well 20F
type: Collection
persistent identity: iGEM 2019 Plate1 Well20F
display id: iGEM_2019_Plate1_Well20F
version: 1
owned by: myers
member: BBa_K611012

iGEM 2019 Distribution Plate 1 Well 13G
type: Collection
persistent identity: iGEM 2019 Plate1 Well13G
display id: iGEM_2019_Plate1_Well13G
version: 1
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member: BBa_K584003

iGEM 2019 Distribution Plate 1 Well 23H
type: Collection
persistent identity: iGEM 2019 Plate1 Well23H
display id: iGEM_2019_Plate1_Well23H
version: 1
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member: BBa_K813002

iGEM 2019 Distribution Plate 1 Well 3G
type: Collection
persistent identity: iGEM 2019 Plate1 Well3G
display id: iGEM_2019_Plate1_Well3G
version: 1
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member: BBa_K538104

iGEM 2019 Distribution Plate 1 Well 7K
type: Collection
persistent identity: iGEM 2019 Plate1 Well7K
display id: iGEM_2019_Plate1_Well7K
version: 1
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member: BBa_K660610

iGEM 2019 Distribution Plate 1 Well 22D
type: Collection
persistent identity: iGEM 2019 Plate1 Well22D
display id: iGEM_2019_Plate1_Well22D
version: 1
owned by: myers
member: BBa_K624001

iGEM 2019 Distribution Plate 1 Well 15O
type: Collection
persistent identity: iGEM 2019 Plate1 Well15O
display id: iGEM_2019_Plate1_Well15O
version: 1
owned by: myers
member: BBa_K592027

iGEM 2019 Distribution Plate 1 Well 4M
type: Collection
persistent identity: iGEM 2019 Plate1 Well4M
display id: iGEM_2019_Plate1_Well4M
version: 1
owned by: myers
member: BBa_K575011

iGEM 2019 Distribution Plate 1 Well 16G
type: Collection
persistent identity: iGEM 2019 Plate1 Well16G
display id: iGEM_2019_Plate1_Well16G
version: 1
owned by: myers
member: BBa_K567013

iGEM 2019 Distribution Plate 1 Well 24D
type: Collection
persistent identity: iGEM 2019 Plate1 Well24D
display id: iGEM_2019_Plate1_Well24D
version: 1
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member: BBa_K539672

iGEM 2019 Distribution Plate 1 Well 14D
type: Collection
persistent identity: iGEM 2019 Plate1 Well14D
display id: iGEM_2019_Plate1_Well14D
version: 1
owned by: myers
member: BBa_K577893

iGEM 2019 Distribution Plate 1 Well 16K
type: Collection
persistent identity: iGEM 2019 Plate1 Well16K
display id: iGEM_2019_Plate1_Well16K
version: 1
owned by: myers
member: BBa_K543020

iGEM 2019 Distribution Plate 1 Well 20O
type: Collection
persistent identity: iGEM 2019 Plate1 Well20O
display id: iGEM_2019_Plate1_Well20O
version: 1
owned by: myers
member: BBa_K823007

iGEM 2019 Distribution Plate 1 Well 24F
type: Collection
persistent identity: iGEM 2019 Plate1 Well24F
display id: iGEM_2019_Plate1_Well24F
version: 1
owned by: myers
member: BBa_K539621

iGEM 2019 Distribution Plate 1 Well 13E
type: Collection
persistent identity: iGEM 2019 Plate1 Well13E
display id: iGEM_2019_Plate1_Well13E
version: 1
owned by: myers
member: BBa_K584002

iGEM 2019 Distribution Plate 1 Well 7E
type: Collection
persistent identity: iGEM 2019 Plate1 Well7E
display id: iGEM_2019_Plate1_Well7E
version: 1
owned by: myers
member: BBa_K515007

iGEM 2019 Distribution Plate 1 Well 18K
type: Collection
persistent identity: iGEM 2019 Plate1 Well18K
display id: iGEM_2019_Plate1_Well18K
version: 1
owned by: myers
member: rhiI

iGEM 2019 Distribution Plate 1 Well 15G
type: Collection
persistent identity: iGEM 2019 Plate1 Well15G
display id: iGEM_2019_Plate1_Well15G
version: 1
owned by: myers
member: pHS

iGEM 2019 Distribution Plate 1 Well 10I
type: Collection
persistent identity: iGEM 2019 Plate1 Well10I
display id: iGEM_2019_Plate1_Well10I
version: 1
owned by: myers
member: BBa_K624003

iGEM 2019 Distribution Plate 1 Well 17N
type: Collection
persistent identity: iGEM 2019 Plate1 Well17N
display id: iGEM_2019_Plate1_Well17N
version: 1
owned by: myers
member: BBa_K864204

iGEM 2019 Distribution Plate 1 Well 1I
type: Collection
persistent identity: iGEM 2019 Plate1 Well1I
display id: iGEM_2019_Plate1_Well1I
version: 1
owned by: myers
member: BBa_K314101

iGEM 2019 Distribution Plate 1 Well 2K
type: Collection
persistent identity: iGEM 2019 Plate1 Well2K
display id: iGEM_2019_Plate1_Well2K
version: 1
owned by: myers
member: BBa_K530025

iGEM 2019 Distribution Plate 1 Well 19O
type: Collection
persistent identity: iGEM 2019 Plate1 Well19O
display id: iGEM_2019_Plate1_Well19O
version: 1
owned by: myers
member: BBa_K530005

iGEM 2019 Distribution Plate 1 Well 8I
type: Collection
persistent identity: iGEM 2019 Plate1 Well8I
display id: iGEM_2019_Plate1_Well8I
version: 1
owned by: myers
member: BBa_K611026

iGEM 2019 Distribution Plate 1 Well 22J
type: Collection
persistent identity: iGEM 2019 Plate1 Well22J
display id: iGEM_2019_Plate1_Well22J
version: 1
owned by: myers
member: BBa_K539626

iGEM 2019 Distribution Plate 1 Well 7I
type: Collection
persistent identity: iGEM 2019 Plate1 Well7I
display id: iGEM_2019_Plate1_Well7I
version: 1
owned by: myers
member: BBa_K523008

iGEM 2019 Distribution Plate 1 Well 15F
type: Collection
persistent identity: iGEM 2019 Plate1 Well15F
display id: iGEM_2019_Plate1_Well15F
version: 1
owned by: myers
member: BBa_K929301

iGEM 2019 Distribution Plate 1 Well 22K
type: Collection
persistent identity: iGEM 2019 Plate1 Well22K
display id: iGEM_2019_Plate1_Well22K
version: 1
owned by: myers
member: BBa_K823013

iGEM 2019 Distribution Plate 1 Well 22B
type: Collection
persistent identity: iGEM 2019 Plate1 Well22B
display id: iGEM_2019_Plate1_Well22B
version: 1
owned by: myers
member: BBa_K642002

BBa_K523003
type: ComponentDefinition
persistent identity: BBa K523003
display id: BBa_K523003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: malS for BioSandwich
creator: Mun Ching Lee, Sylvia Ispasanie
created: 2011-07-28T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a version of BBa_K523001 but with no RBS and no stop codon. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. This part is intended to be used with Edinburgh 2011's "BioSandwich" protocol, in which a BglII site before the coding sequence, and a SpeI site after the coding sequence (i.e. the one in the suffix) are used for assembly.
mutable notes: The part was made using the strategy outlined in BBa_K523000 , and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. The part was created from a lab ''E. coli'' strain using primers: * ACT AGATCT atg aaa ctc gcc gcc tgt ttt c ** Forward; adds BglII site; starts at start codon. *ACT ACTAGT ACC ctg ttg ccc tgc cca gac g ** Reverse; adds SpeI site; RFC23 and BioSandwich compliant; no stop codons, adds a glycine.
mutable provenance: See BBa_K523001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: malS
sequence annotation: ATG
sequence annotation: BglII
sequence annotation: GG
sequence: https://synbiohub.org/public/igem/BBa_K523003_sequence/1

malS
type: SequenceAnnotation
persistent identity: annotation2124167
display id: annotation2124167
version: 1
top level: BBa_K523003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523003/annotation2124167/range2124167/1

https://synbiohub.org/public/igem/BBa_K523003/annotation2124167/range2124167/1
type: Range
persistent identity: range2124167
display id: range2124167
version: 1
direction: direction/forward
top level: BBa_K523003
start: 5
end: 2032
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2124172
display id: annotation2124172
version: 1
top level: BBa_K523003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523003/annotation2124172/range2124172/1

https://synbiohub.org/public/igem/BBa_K523003/annotation2124172/range2124172/1
type: Range
persistent identity: range2124172
display id: range2124172
version: 1
direction: direction/forward
top level: BBa_K523003
start: 5
end: 5
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation2124169
display id: annotation2124169
version: 1
top level: BBa_K523003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523003/annotation2124169/range2124169/1

https://synbiohub.org/public/igem/BBa_K523003/annotation2124169/range2124169/1
type: Range
persistent identity: range2124169
display id: range2124169
version: 1
direction: direction/forward
top level: BBa_K523003
start: 1
end: 4
orientation: inline

GG
type: SequenceAnnotation
persistent identity: annotation2124168
display id: annotation2124168
version: 1
top level: BBa_K523003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523003/annotation2124168/range2124168/1

https://synbiohub.org/public/igem/BBa_K523003/annotation2124168/range2124168/1
type: Range
persistent identity: range2124168
display id: range2124168
version: 1
direction: direction/forward
top level: BBa_K523003
start: 2033
end: 2034
orientation: inline

BBa_K606061
type: ComponentDefinition
persistent identity: BBa K606061
display id: BBa_K606061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpoVG Ribosome Binding Site (RBS) for B. subtilis
creator: Axel S??guret
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: SpoVG Ribosome Binding Site (RBS) for B. subtilis
mutable notes: RBS
mutable provenance: SYnthesized RBS because part BBa_K143021 was wrong.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SpoVG
sequence: https://synbiohub.org/public/igem/BBa_K606061_sequence/1

SpoVG
type: SequenceAnnotation
persistent identity: annotation2160544
display id: annotation2160544
version: 1
top level: BBa_K606061
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K606061/annotation2160544/range2160544/1

https://synbiohub.org/public/igem/BBa_K606061/annotation2160544/range2160544/1
type: Range
persistent identity: range2160544
display id: range2160544
version: 1
direction: direction/forward
top level: BBa_K606061
start: 1
end: 12
orientation: inline

BBa_K516031
type: ComponentDefinition
persistent identity: BBa K516031
display id: BBa_K516031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP protein generator TERM+ RBS+(B0031)
creator: Nicolo' Politi, Federica Sampietro, Davide Bianchini
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: mRFP protein generator TERM+ RBS+(B0031)
mutable notes: --
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0015
component: BBa_B0031
sequence annotation: BBa_B0015
sequence annotation: BBa_E1010
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_K516031_sequence/1

BBa_E1010
type: Component
persistent identity: component2250588
display id: component2250588
version: 1
top level: BBa_K516031
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0015
type: Component
persistent identity: component2250595
display id: component2250595
version: 1
top level: BBa_K516031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0031
type: Component
persistent identity: component2250585
display id: component2250585
version: 1
top level: BBa_K516031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250595
display id: annotation2250595
version: 1
top level: BBa_K516031
location: https://synbiohub.org/public/igem/BBa_K516031/annotation2250595/range2250595/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516031/annotation2250595/range2250595/1
type: Range
persistent identity: range2250595
display id: range2250595
version: 1
direction: direction/reverse
top level: BBa_K516031
start: 735
end: 863
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250588
display id: annotation2250588
version: 1
top level: BBa_K516031
location: https://synbiohub.org/public/igem/BBa_K516031/annotation2250588/range2250588/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K516031/annotation2250588/range2250588/1
type: Range
persistent identity: range2250588
display id: range2250588
version: 1
direction: direction/reverse
top level: BBa_K516031
start: 21
end: 726
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2250585
display id: annotation2250585
version: 1
top level: BBa_K516031
location: https://synbiohub.org/public/igem/BBa_K516031/annotation2250585/range2250585/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K516031/annotation2250585/range2250585/1
type: Range
persistent identity: range2250585
display id: range2250585
version: 1
direction: direction/reverse
top level: BBa_K516031
start: 1
end: 14
orientation: inline

CspC
type: ComponentDefinition
persistent identity: BBa K538004
display id: BBa_K538004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CspC (P. irgensii)
creator: Bas Stringer (Sequence by Paul van Dieken)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Double Stop Codon
sequence annotation: RNA binding motif (RPN1)
sequence annotation: RNA binding motif (RPN2)
sequence annotation: Start Codon
sequence annotation: A > G (Recode for SapI site)
sequence: https://synbiohub.org/public/igem/BBa_K538004_sequence/1

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2127784
display id: annotation2127784
version: 1
top level: CspC
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127784/range2127784/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2127784/range2127784/1
type: Range
persistent identity: range2127784
display id: range2127784
version: 1
direction: direction/forward
top level: CspC
start: 190
end: 195
orientation: inline

RNA binding motif (RPN1)
type: SequenceAnnotation
persistent identity: annotation2129605
display id: annotation2129605
version: 1
top level: CspC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2129605/range2129605/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2129605/range2129605/1
type: Range
persistent identity: range2129605
display id: range2129605
version: 1
direction: direction/forward
top level: CspC
start: 37
end: 54
orientation: inline

RNA binding motif (RPN2)
type: SequenceAnnotation
persistent identity: annotation2129606
display id: annotation2129606
version: 1
top level: CspC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2129606/range2129606/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2129606/range2129606/1
type: Range
persistent identity: range2129606
display id: range2129606
version: 1
direction: direction/forward
top level: CspC
start: 79
end: 93
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2127783
display id: annotation2127783
version: 1
top level: CspC
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127783/range2127783/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2127783/range2127783/1
type: Range
persistent identity: range2127783
display id: range2127783
version: 1
direction: direction/forward
top level: CspC
start: 1
end: 3
orientation: inline

A > G (Recode for SapI site)
type: SequenceAnnotation
persistent identity: annotation2127785
display id: annotation2127785
version: 1
top level: CspC
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127785/range2127785/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2127785/range2127785/1
type: Range
persistent identity: range2127785
display id: range2127785
version: 1
direction: direction/forward
top level: CspC
start: 75
end: 75
orientation: inline

BBa_K517005
type: ComponentDefinition
persistent identity: BBa K517005
display id: BBa_K517005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: erg20-2
creator: Gurpal Bisra
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _682_
m user id: 0
owner id: 7732
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A mutant of the ERG20 gene; K197E substitution. The encoded mutant protein produces 25% FPP and 75% GPP instead of 75% FPP and 25% GPP compared to the wild type Erg20. GPP is a precursor in producing monoterpenes whereas FPP produces diterpenoids instead. Reference: Oswald M., Fischer M., Dirninger N., Karst F. Monoterpenoid biosynthesis in Saccharomyces cerevisiae. 2007.
mutable notes: None
mutable provenance: Saccharomyces cerevisiae yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K517005_sequence/1

BBa_K864101
type: ComponentDefinition
persistent identity: BBa K864101
display id: BBa_K864101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032-SYFP2
creator: Arvid Hed??n Gynn??
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 13993
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the BBa_K864100 ( BBa_K864100 ) with the medium strength BBa_B0032 RBS. The B0032 RBS is commonly used for promoter testing, giving a wider dynamic range than a stronger RBS.
mutable notes: Transcriptional leakage is present in some backbones, giving visibly fluorescent colonies even without promoter.
mutable provenance: Assembly of K864100 and the B0032 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K864100
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K864100
sequence: https://synbiohub.org/public/igem/BBa_K864101_sequence/1

BBa_K864100
type: Component
persistent identity: component2197148
display id: component2197148
version: 1
top level: BBa_K864101
access: public
definition: SYFP2

BBa_B0032
type: Component
persistent identity: component2197145
display id: component2197145
version: 1
top level: BBa_K864101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2197145
display id: annotation2197145
version: 1
top level: BBa_K864101
location: https://synbiohub.org/public/igem/BBa_K864101/annotation2197145/range2197145/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K864101/annotation2197145/range2197145/1
type: Range
persistent identity: range2197145
display id: range2197145
version: 1
direction: direction/forward
top level: BBa_K864101
start: 1
end: 13
orientation: inline

BBa_K864100
type: SequenceAnnotation
persistent identity: annotation2197148
display id: annotation2197148
version: 1
top level: BBa_K864101
location: https://synbiohub.org/public/igem/BBa_K864101/annotation2197148/range2197148/1
component: BBa_K864100

https://synbiohub.org/public/igem/BBa_K864101/annotation2197148/range2197148/1
type: Range
persistent identity: range2197148
display id: range2197148
version: 1
direction: direction/reverse
top level: BBa_K864101
start: 20
end: 742
orientation: inline

BBa_K515007
type: ComponentDefinition
persistent identity: BBa K515007
display id: BBa_K515007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dendra2
creator: Atipat Patharagulpong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: atgaacctcatcaaagaggatatgcgcgtaaaagtccacatggaaggcaacgtgaatggtcatgcgttcgtaatcgaagg tgaaggcaaagggaaaccgtacgaaggtacgcaaactgcaaacttgaccgtgaaagaaggtgcacctctgccattcagct acgacattcttaccactgcggttcactatggcaatcgtgtctttacgaaatatccggaagatattccggactacttcaaa cagtcgtttccggaaggctattcatgggagcgtaccatgacctttgaggataaaggcatttgcacaattcgcagcgatat tagcctggaaggggactgcttctttcagaacgttcggtttaaaggcacgaattttcctccgaatgggccggttatgcaga agaaaaccctgaaatgggaaccgtcgacagagaaactgcatgtgcgtgatggacttctggtcggtaacatcaacatggcc ttactgttggaaggtggaggccattatctctgtgacttcaaaaccacgtataaggcgaagaaagtggtacagctgccaga tgcccactttgtggatcatcgcatcgaaattctgggcaatgactccgattacaacaaagtcaagctgtacgagcatgctg ttgctcgctatagtcccttaccctctcaagtgtggtgataa
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dendra 2
sequence: https://synbiohub.org/public/igem/BBa_K515007_sequence/1

Dendra 2
type: SequenceAnnotation
persistent identity: annotation2127295
display id: annotation2127295
version: 1
top level: BBa_K515007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K515007/annotation2127295/range2127295/1

https://synbiohub.org/public/igem/BBa_K515007/annotation2127295/range2127295/1
type: Range
persistent identity: range2127295
display id: range2127295
version: 1
direction: direction/forward
top level: BBa_K515007
start: 1
end: 681
orientation: inline

rhiI
type: ComponentDefinition
persistent identity: BBa K594005
display id: BBa_K594005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a luxI homologous AHL synthetase from Rhzobium leguminosarum bv. vicae IMA57
creator: Yu Xiaoyan
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _765_
m user id: 0
owner id: 8221
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R. leguminosarum bv. viciae is a best characterized organism in the nitrogen-fixing rhizobia. Several quorum-sensing systems (rai, rhi, cin,and tra) have been identified and are intertwined in a complex regulatory network. The rhi system is composed of rhiR (a luxR homolog),rhiI (a luxI homolog), and the rhiABC operon, all of which are located on the symbiotic plasmid pRL1JI (29, 139). It was demonstrated that rhiABC was controlled by RhiR and that flavonoids repressed the expression of both rhiR and rhiABC.
mutable notes: Initial experiments showed 3-OH-C14:1-HSL to be an inducer of the rhi genes, but later data showing that rhiI and rhiA could be induced by short-chain AHLs (C6-HSL, C8-HSL, and 3-oxo-C8-HSL) but not 3-OH-C14:1-HSL suggested that the induction was indirect.
mutable provenance: it is from the Rhzobium leguminosarum bv. vicae IMA57 we purchased from the CGMCC(China General Microbiological Culture Collection Center).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhiI
sequence: https://synbiohub.org/public/igem/BBa_K594005_sequence/1

rhiI
type: SequenceAnnotation
persistent identity: annotation2148051
display id: annotation2148051
version: 1
top level: rhiI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K594005/annotation2148051/range2148051/1

https://synbiohub.org/public/igem/BBa_K594005/annotation2148051/range2148051/1
type: Range
persistent identity: range2148051
display id: range2148051
version: 1
direction: direction/forward
top level: rhiI
start: 1
end: 552
orientation: inline

BBa_K895004
type: ComponentDefinition
persistent identity: BBa K895004
display id: BBa_K895004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salty_Hcp-CD27_endolysin
creator: Frank Sargent
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1160_
m user id: 0
owner id: 8083
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a coding part comprising the full open reading frame of Salmonella enterica LT2 hcp gene (also known as STM0289 or tssD) linked via an haemagglutinin eptitope tag to a synthetic gene encoding an endolysin from the CD27 bacteriophage. The CD27 endolysin is believed to have high specificity against the cell wall of Clostridium difficile.
mutable notes: None in this case.
mutable provenance: The hcp section is derived from BBa_K895001 , which was used as a PCR template, and the endolysin section is derived from a plasmid constructed by Neil Fairweather's group at Imperial College London.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: endolysin
sequence annotation: hcp
sequence annotation: HA tag
sequence: https://synbiohub.org/public/igem/BBa_K895004_sequence/1

endolysin
type: SequenceAnnotation
persistent identity: annotation2193790
display id: annotation2193790
version: 1
top level: BBa_K895004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895004/annotation2193790/range2193790/1

https://synbiohub.org/public/igem/BBa_K895004/annotation2193790/range2193790/1
type: Range
persistent identity: range2193790
display id: range2193790
version: 1
direction: direction/forward
top level: BBa_K895004
start: 517
end: 1089
orientation: inline

hcp
type: SequenceAnnotation
persistent identity: annotation2193789
display id: annotation2193789
version: 1
top level: BBa_K895004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895004/annotation2193789/range2193789/1

https://synbiohub.org/public/igem/BBa_K895004/annotation2193789/range2193789/1
type: Range
persistent identity: range2193789
display id: range2193789
version: 1
direction: direction/forward
top level: BBa_K895004
start: 1
end: 483
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2193788
display id: annotation2193788
version: 1
top level: BBa_K895004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K895004/annotation2193788/range2193788/1

https://synbiohub.org/public/igem/BBa_K895004/annotation2193788/range2193788/1
type: Range
persistent identity: range2193788
display id: range2193788
version: 1
direction: direction/forward
top level: BBa_K895004
start: 484
end: 510
orientation: inline

BBa_K620000
type: ComponentDefinition
persistent identity: BBa K620000
display id: BBa_K620000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DDT Dehydrochlorinase
creator: Caltech iGEM 2011
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _792_
m user id: 0
owner id: 9568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Also known as Glutathione S-transferase 1-1, this protein is supposed to degrade DDT, an endocrine disruptor and persistent organic pollutant.
mutable notes: The part was assembled through PCR using a set of long oligos. The sequence contains an NdeI site, which made it more difficult to place into a pET11a vector for protein purification and characterization.
mutable provenance: This part was isolated from Anopheles dirus. The team took the amino acid sequence found in the NCBI protein database and used reverse transcribed using codon optimization for E. coli to create the DNA part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double stop codon
sequence annotation: K620000
sequence: https://synbiohub.org/public/igem/BBa_K620000_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2135663
display id: annotation2135663
version: 1
top level: BBa_K620000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K620000/annotation2135663/range2135663/1

https://synbiohub.org/public/igem/BBa_K620000/annotation2135663/range2135663/1
type: Range
persistent identity: range2135663
display id: range2135663
version: 1
direction: direction/forward
top level: BBa_K620000
start: 628
end: 633
orientation: inline

K620000
type: SequenceAnnotation
persistent identity: annotation2135664
display id: annotation2135664
version: 1
top level: BBa_K620000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K620000/annotation2135664/range2135664/1

https://synbiohub.org/public/igem/BBa_K620000/annotation2135664/range2135664/1
type: Range
persistent identity: range2135664
display id: range2135664
version: 1
direction: direction/forward
top level: BBa_K620000
start: 1
end: 633
orientation: inline

BBa_K539731
type: ComponentDefinition
persistent identity: BBa K539731
display id: BBa_K539731
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kivd (alpha-ketoisovalerate decarboxylase) under TetR repressible promoter
creator: Yang, Yi-Pei
created: 2011-09-23T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence of kivd from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This is part of the isobutanol biosynthesis pathway. This part kivd is under TetR repressible promoter that constitutively ON and repressed by TetR.
mutable notes: It is a working biobrick.
mutable provenance: kivd is cloned form Lactococcus lactis subsp. lactis KF147
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K539714
component: BBa_B0034
sequence annotation: BBa_K539714
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K539731_sequence/1

BBa_R0040
type: Component
persistent identity: component2219940
display id: component2219940
version: 1
top level: BBa_K539731
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K539714
type: Component
persistent identity: component2219948
display id: component2219948
version: 1
top level: BBa_K539731
access: public
definition: https://synbiohub.org/public/igem/BBa_K539714/1

BBa_B0034
type: Component
persistent identity: component2219946
display id: component2219946
version: 1
top level: BBa_K539731
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K539714
type: SequenceAnnotation
persistent identity: annotation2219948
display id: annotation2219948
version: 1
top level: BBa_K539731
location: https://synbiohub.org/public/igem/BBa_K539731/annotation2219948/range2219948/1
component: BBa_K539714

https://synbiohub.org/public/igem/BBa_K539731/annotation2219948/range2219948/1
type: Range
persistent identity: range2219948
display id: range2219948
version: 1
direction: direction/reverse
top level: BBa_K539731
start: 81
end: 1727
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2219940
display id: annotation2219940
version: 1
top level: BBa_K539731
location: https://synbiohub.org/public/igem/BBa_K539731/annotation2219940/range2219940/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K539731/annotation2219940/range2219940/1
type: Range
persistent identity: range2219940
display id: range2219940
version: 1
direction: direction/forward
top level: BBa_K539731
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219946
display id: annotation2219946
version: 1
top level: BBa_K539731
location: https://synbiohub.org/public/igem/BBa_K539731/annotation2219946/range2219946/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K539731/annotation2219946/range2219946/1
type: Range
persistent identity: range2219946
display id: range2219946
version: 1
direction: direction/reverse
top level: BBa_K539731
start: 63
end: 74
orientation: inline

BBa_K641009
type: ComponentDefinition
persistent identity: BBa K641009
display id: BBa_K641009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P_rffGH.rbs.ToxR-Nhe1.{gsgsgs}{LambdaRep}
creator: Spencer Scott
created: 2011-08-31T11:00:00Z
modified: 2015-05-08T01:12:58Z
discontinued: false
dominant: false
group u list: _817_
m user id: 0
owner id: 9470
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This composite part constitutively expresses a ToxR-LambdaRep construct. This ToxR construct will dimerize at a high affinity, because of Lambda Rep's dimerizing properties, making the cytoplasmic ToxR dimer an active transcription factor for the ctx Promoter. Thus, If this composite part is combined with a Pctx-GFP reporter there will be a high expression of GFP.
mutable notes: The biggest design considerations were how to limit the amount of ToxR expressed while still allowing enough ToxR to be produced to see a high expression of GFP. The P_rffGH promoter, the rbs, and the plasmid we put it on (ColE1 origin) all contributed to the optimal expression level of the ToxR construct.
mutable provenance: The original promoter came from E. Coli genome, but this specific promoter was selected from a library of that promoter. This rbs was selected from a library in a tuning process. ToxR comes from a virulence island of Vibrio Cholerae. The Lambda Repressor is from the Lambda Phage.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K641009_sequence/1

BBa_K575025
type: ComponentDefinition
persistent identity: BBa K575025
display id: BBa_K575025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0034) + LasR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of LasR combined with receptor
mutable notes: put the constitutive promoter after the production of LasR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575008
component: BBa_K575005
sequence annotation: BBa_K575005
sequence annotation: BBa_K575008
sequence: https://synbiohub.org/public/igem/BBa_K575025_sequence/1

BBa_K575008
type: Component
persistent identity: component2138223
display id: component2138223
version: 1
top level: BBa_K575025
access: public
definition: BBa_K575008

BBa_K575005
type: Component
persistent identity: component2138228
display id: component2138228
version: 1
top level: BBa_K575025
access: public
definition: https://synbiohub.org/public/igem/BBa_K575005/1

BBa_K575005
type: SequenceAnnotation
persistent identity: annotation2138228
display id: annotation2138228
version: 1
top level: BBa_K575025
location: https://synbiohub.org/public/igem/BBa_K575025/annotation2138228/range2138228/1
component: BBa_K575005

https://synbiohub.org/public/igem/BBa_K575025/annotation2138228/range2138228/1
type: Range
persistent identity: range2138228
display id: range2138228
version: 1
direction: direction/reverse
top level: BBa_K575025
start: 915
end: 1698
orientation: inline

BBa_K575008
type: SequenceAnnotation
persistent identity: annotation2138223
display id: annotation2138223
version: 1
top level: BBa_K575025
location: https://synbiohub.org/public/igem/BBa_K575025/annotation2138223/range2138223/1
component: BBa_K575008

https://synbiohub.org/public/igem/BBa_K575025/annotation2138223/range2138223/1
type: Range
persistent identity: range2138223
display id: range2138223
version: 1
direction: direction/reverse
top level: BBa_K575025
start: 1
end: 906
orientation: inline

BBa_K575037
type: ComponentDefinition
persistent identity: BBa K575037
display id: BBa_K575037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP + Constitutive Promoter + RBS (B0034) + RhlR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of RhlR combined with receptor with RhlP.
mutable notes: put the constitutive promoter after the production of RhlR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575007
component: BBa_K575013
sequence annotation: BBa_K575007
sequence annotation: BBa_K575013
sequence: https://synbiohub.org/public/igem/BBa_K575037_sequence/1

BBa_K575007
type: Component
persistent identity: component2138454
display id: component2138454
version: 1
top level: BBa_K575037
access: public
definition: https://synbiohub.org/public/igem/BBa_K575007/1

BBa_K575013
type: Component
persistent identity: component2138449
display id: component2138449
version: 1
top level: BBa_K575037
access: public
definition: BBa_K575013

BBa_K575007
type: SequenceAnnotation
persistent identity: annotation2138454
display id: annotation2138454
version: 1
top level: BBa_K575037
location: https://synbiohub.org/public/igem/BBa_K575037/annotation2138454/range2138454/1
component: BBa_K575007

https://synbiohub.org/public/igem/BBa_K575037/annotation2138454/range2138454/1
type: Range
persistent identity: range2138454
display id: range2138454
version: 1
direction: direction/reverse
top level: BBa_K575037
start: 808
end: 1597
orientation: inline

BBa_K575013
type: SequenceAnnotation
persistent identity: annotation2138449
display id: annotation2138449
version: 1
top level: BBa_K575037
location: https://synbiohub.org/public/igem/BBa_K575037/annotation2138449/range2138449/1
component: BBa_K575013

https://synbiohub.org/public/igem/BBa_K575037/annotation2138449/range2138449/1
type: Range
persistent identity: range2138449
display id: range2138449
version: 1
direction: direction/reverse
top level: BBa_K575037
start: 1
end: 799
orientation: inline

BBa_K648029
type: ComponentDefinition
persistent identity: BBa K648029
display id: BBa_K648029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OR, operating region for Lambda switch
creator: James Coletta, Anisha Katyal, Kristen Salavo, Lauren Rossi
created: 2011-07-24T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _825_
m user id: 0
owner id: 9891
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The operating region for the Lambda switch that represses C1 and Cro bind to. This region contains promoters prm and pr facing in opposite directions. It also contains operating regions Or3, Or2 and Or1. In the lysogenic state, the C1 dimer is bound naturally bound to the Or1 repressing promotor pr and production of cro. Once RecA cleaves enough C1 dimer the system switches to the lytic state. The Cro produced at the pr promoter then binds to the Or3 site inhibiting the prm promoter. For a better description of the Lambda system read the book "A genetic switch" by Mark Ptashne. We used Or in creation of the lambda system, as part of our project to create a sensor for DNA damage in bacteria due to radiation.
mutable provenance: Galen Lynch notebook, iGEM 2007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K648029_sequence/1

BBa_K575026
type: ComponentDefinition
persistent identity: BBa K575026
display id: BBa_K575026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0030) + LasR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of LasR combined with receptor
mutable notes: put the constitutive promoter after the production of LasR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575004
component: BBa_K575010
sequence annotation: BBa_K575004
sequence annotation: BBa_K575010
sequence: https://synbiohub.org/public/igem/BBa_K575026_sequence/1

BBa_K575004
type: Component
persistent identity: component2264127
display id: component2264127
version: 1
top level: BBa_K575026
access: public
definition: https://synbiohub.org/public/igem/BBa_K575004/1

BBa_K575010
type: Component
persistent identity: component2264121
display id: component2264121
version: 1
top level: BBa_K575026
access: public
definition: BBa_K575010

BBa_K575004
type: SequenceAnnotation
persistent identity: annotation2264127
display id: annotation2264127
version: 1
top level: BBa_K575026
location: https://synbiohub.org/public/igem/BBa_K575026/annotation2264127/range2264127/1
component: BBa_K575004

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type: Range
persistent identity: range2264127
display id: range2264127
version: 1
direction: direction/reverse
top level: BBa_K575026
start: 901
end: 1687
orientation: inline

BBa_K575010
type: SequenceAnnotation
persistent identity: annotation2264121
display id: annotation2264121
version: 1
top level: BBa_K575026
location: https://synbiohub.org/public/igem/BBa_K575026/annotation2264121/range2264121/1
component: BBa_K575010

https://synbiohub.org/public/igem/BBa_K575026/annotation2264121/range2264121/1
type: Range
persistent identity: range2264121
display id: range2264121
version: 1
direction: direction/reverse
top level: BBa_K575026
start: 1
end: 892
orientation: inline

BBa_K314101
type: ComponentDefinition
persistent identity: BBa K314101
display id: BBa_K314101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low constitutive expression cassette
creator: Tomas Huber, Justin Siegel
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Medium constitutive protein expression insert includes f1 origin and RBS B0034
mutable provenance: construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K314110
component: BBa_J23114
sequence annotation: BBa_J23114
sequence annotation: BBa_K314110
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K314101_sequence/1

BBa_B0034
type: Component
persistent identity: component2091075
display id: component2091075
version: 1
top level: BBa_K314101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K314110
type: Component
persistent identity: component2091072
display id: component2091072
version: 1
top level: BBa_K314101
access: public
definition: f1 ori

BBa_J23114
type: Component
persistent identity: component2091073
display id: component2091073
version: 1
top level: BBa_K314101
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2091073
display id: annotation2091073
version: 1
top level: BBa_K314101
location: https://synbiohub.org/public/igem/BBa_K314101/annotation2091073/range2091073/1
component: BBa_J23114

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type: Range
persistent identity: range2091073
display id: range2091073
version: 1
direction: direction/reverse
top level: BBa_K314101
start: 464
end: 498
orientation: inline

BBa_K314110
type: SequenceAnnotation
persistent identity: annotation2091072
display id: annotation2091072
version: 1
top level: BBa_K314101
location: https://synbiohub.org/public/igem/BBa_K314101/annotation2091072/range2091072/1
component: BBa_K314110

https://synbiohub.org/public/igem/BBa_K314101/annotation2091072/range2091072/1
type: Range
persistent identity: range2091072
display id: range2091072
version: 1
direction: direction/reverse
top level: BBa_K314101
start: 1
end: 455
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2091075
display id: annotation2091075
version: 1
top level: BBa_K314101
location: https://synbiohub.org/public/igem/BBa_K314101/annotation2091075/range2091075/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K314101/annotation2091075/range2091075/1
type: Range
persistent identity: range2091075
display id: range2091075
version: 1
direction: direction/reverse
top level: BBa_K314101
start: 507
end: 518
orientation: inline

BBa_K523008
type: ComponentDefinition
persistent identity: BBa K523008
display id: BBa_K523008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: INP for BioSandwich
creator: Mun Ching Lee, Sylvia Ispasanie
created: 2011-08-03T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a modified version of BBa_K265008 for use with the BioSandwich protocol of Edinburgh 2011.
mutable notes: N/A.
mutable provenance: From BBa_K265008 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: INPNC
sequence annotation: BglII
sequence annotation: GG
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K523008_sequence/1

INPNC
type: SequenceAnnotation
persistent identity: annotation2124328
display id: annotation2124328
version: 1
top level: BBa_K523008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523008/annotation2124328/range2124328/1

https://synbiohub.org/public/igem/BBa_K523008/annotation2124328/range2124328/1
type: Range
persistent identity: range2124328
display id: range2124328
version: 1
direction: direction/forward
top level: BBa_K523008
start: 5
end: 928
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation2124325
display id: annotation2124325
version: 1
top level: BBa_K523008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523008/annotation2124325/range2124325/1

https://synbiohub.org/public/igem/BBa_K523008/annotation2124325/range2124325/1
type: Range
persistent identity: range2124325
display id: range2124325
version: 1
direction: direction/forward
top level: BBa_K523008
start: 1
end: 4
orientation: inline

GG
type: SequenceAnnotation
persistent identity: annotation2124327
display id: annotation2124327
version: 1
top level: BBa_K523008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523008/annotation2124327/range2124327/1

https://synbiohub.org/public/igem/BBa_K523008/annotation2124327/range2124327/1
type: Range
persistent identity: range2124327
display id: range2124327
version: 1
direction: direction/forward
top level: BBa_K523008
start: 929
end: 930
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2124326
display id: annotation2124326
version: 1
top level: BBa_K523008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523008/annotation2124326/range2124326/1

https://synbiohub.org/public/igem/BBa_K523008/annotation2124326/range2124326/1
type: Range
persistent identity: range2124326
display id: range2124326
version: 1
direction: direction/forward
top level: BBa_K523008
start: 5
end: 5
orientation: inline

BBa_K584001
type: ComponentDefinition
persistent identity: BBa K584001
display id: BBa_K584001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter + GFP generator
creator: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
created: 2011-08-15T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _755_
m user id: 0
owner id: 9879
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part was designed to test the functionality of the constitutive promotor region.
mutable notes: /
mutable provenance: /
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23119
component: BBa_E0240
sequence annotation: BBa_J23119
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K584001_sequence/1

BBa_J23119
type: Component
persistent identity: component2125132
display id: component2125132
version: 1
top level: BBa_K584001
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_E0240
type: Component
persistent identity: component2125143
display id: component2125143
version: 1
top level: BBa_K584001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2125132
display id: annotation2125132
version: 1
top level: BBa_K584001
location: https://synbiohub.org/public/igem/BBa_K584001/annotation2125132/range2125132/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K584001/annotation2125132/range2125132/1
type: Range
persistent identity: range2125132
display id: range2125132
version: 1
direction: direction/reverse
top level: BBa_K584001
start: 1
end: 35
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2125143
display id: annotation2125143
version: 1
top level: BBa_K584001
location: https://synbiohub.org/public/igem/BBa_K584001/annotation2125143/range2125143/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K584001/annotation2125143/range2125143/1
type: Range
persistent identity: range2125143
display id: range2125143
version: 1
direction: direction/reverse
top level: BBa_K584001
start: 44
end: 919
orientation: inline

BBa_K523022
type: ComponentDefinition
persistent identity: BBa K523022
display id: BBa_K523022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlacLacZ + crtEIB
creator: Mun Ching Lee, Sylvia Ispasanie
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lac promoter driving expression of crtEIB cluster.
mutable notes: BioSandwich assembly of other parts.
mutable provenance: BioSandwich assembly of other parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TAG
sequence annotation: Plac - LacZ
sequence annotation: TGA
sequence annotation: Spacer
sequence annotation: TAA
sequence annotation: crtI
sequence annotation: rbs
sequence annotation: ATG
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacZ
sequence annotation: crtB
sequence annotation: crtE
sequence: https://synbiohub.org/public/igem/BBa_K523022_sequence/1

TAG
type: SequenceAnnotation
persistent identity: annotation2129079
display id: annotation2129079
version: 1
top level: BBa_K523022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129079/range2129079/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129079/range2129079/1
type: Range
persistent identity: range2129079
display id: range2129079
version: 1
direction: direction/forward
top level: BBa_K523022
start: 4038
end: 4038
orientation: inline

Plac - LacZ
type: SequenceAnnotation
persistent identity: annotation2129059
display id: annotation2129059
version: 1
top level: BBa_K523022
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129059/range2129059/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129059/range2129059/1
type: Range
persistent identity: range2129059
display id: range2129059
version: 1
direction: direction/forward
top level: BBa_K523022
start: 38
end: 589
orientation: inline

TGA
type: SequenceAnnotation
persistent identity: annotation2129075
display id: annotation2129075
version: 1
top level: BBa_K523022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129075/range2129075/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129075/range2129075/1
type: Range
persistent identity: range2129075
display id: range2129075
version: 1
direction: direction/forward
top level: BBa_K523022
start: 3066
end: 3066
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2129061
display id: annotation2129061
version: 1
top level: BBa_K523022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129061/range2129061/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129061/range2129061/1
type: Range
persistent identity: range2129061
display id: range2129061
version: 1
direction: direction/forward
top level: BBa_K523022
start: 1
end: 32
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129071
display id: annotation2129071
version: 1
top level: BBa_K523022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129071/range2129071/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129071/range2129071/1
type: Range
persistent identity: range2129071
display id: range2129071
version: 1
direction: direction/forward
top level: BBa_K523022
start: 1556
end: 1556
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2129076
display id: annotation2129076
version: 1
top level: BBa_K523022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129076/range2129076/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129076/range2129076/1
type: Range
persistent identity: range2129076
display id: range2129076
version: 1
direction: direction/forward
top level: BBa_K523022
start: 3066
end: 3095
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2129072
display id: annotation2129072
version: 1
top level: BBa_K523022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129072/range2129072/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129072/range2129072/1
type: Range
persistent identity: range2129072
display id: range2129072
version: 1
direction: direction/forward
top level: BBa_K523022
start: 1547
end: 1574
orientation: inline

crtI
type: SequenceAnnotation
persistent identity: annotation2129074
display id: annotation2129074
version: 1
top level: BBa_K523022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129074/range2129074/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129074/range2129074/1
type: Range
persistent identity: range2129074
display id: range2129074
version: 1
direction: direction/forward
top level: BBa_K523022
start: 1572
end: 3068
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129077
display id: annotation2129077
version: 1
top level: BBa_K523022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129077/range2129077/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129077/range2129077/1
type: Range
persistent identity: range2129077
display id: range2129077
version: 1
direction: direction/forward
top level: BBa_K523022
start: 3088
end: 3091
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129069
display id: annotation2129069
version: 1
top level: BBa_K523022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129069/range2129069/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129069/range2129069/1
type: Range
persistent identity: range2129069
display id: range2129069
version: 1
direction: direction/forward
top level: BBa_K523022
start: 623
end: 623
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2129063
display id: annotation2129063
version: 1
top level: BBa_K523022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129063/range2129063/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129063/range2129063/1
type: Range
persistent identity: range2129063
display id: range2129063
version: 1
direction: direction/forward
top level: BBa_K523022
start: 286
end: 291
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2129064
display id: annotation2129064
version: 1
top level: BBa_K523022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129064/range2129064/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129064/range2129064/1
type: Range
persistent identity: range2129064
display id: range2129064
version: 1
direction: direction/forward
top level: BBa_K523022
start: 309
end: 314
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2129062
display id: annotation2129062
version: 1
top level: BBa_K523022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129062/range2129062/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129062/range2129062/1
type: Range
persistent identity: range2129062
display id: range2129062
version: 1
direction: direction/forward
top level: BBa_K523022
start: 359
end: 589
orientation: inline

crtB
type: SequenceAnnotation
persistent identity: annotation2129078
display id: annotation2129078
version: 1
top level: BBa_K523022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129078/range2129078/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129078/range2129078/1
type: Range
persistent identity: range2129078
display id: range2129078
version: 1
direction: direction/forward
top level: BBa_K523022
start: 3102
end: 4040
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2129067
display id: annotation2129067
version: 1
top level: BBa_K523022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129067/range2129067/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129067/range2129067/1
type: Range
persistent identity: range2129067
display id: range2129067
version: 1
direction: direction/forward
top level: BBa_K523022
start: 597
end: 625
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129068
display id: annotation2129068
version: 1
top level: BBa_K523022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129068/range2129068/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129068/range2129068/1
type: Range
persistent identity: range2129068
display id: range2129068
version: 1
direction: direction/forward
top level: BBa_K523022
start: 611
end: 614
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129082
display id: annotation2129082
version: 1
top level: BBa_K523022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129082/range2129082/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129082/range2129082/1
type: Range
persistent identity: range2129082
display id: range2129082
version: 1
direction: direction/forward
top level: BBa_K523022
start: 1560
end: 1563
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129073
display id: annotation2129073
version: 1
top level: BBa_K523022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129073/range2129073/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129073/range2129073/1
type: Range
persistent identity: range2129073
display id: range2129073
version: 1
direction: direction/forward
top level: BBa_K523022
start: 1572
end: 1572
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129081
display id: annotation2129081
version: 1
top level: BBa_K523022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129081/range2129081/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129081/range2129081/1
type: Range
persistent identity: range2129081
display id: range2129081
version: 1
direction: direction/forward
top level: BBa_K523022
start: 3102
end: 3102
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129065
display id: annotation2129065
version: 1
top level: BBa_K523022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129065/range2129065/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129065/range2129065/1
type: Range
persistent identity: range2129065
display id: range2129065
version: 1
direction: direction/forward
top level: BBa_K523022
start: 348
end: 351
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2129080
display id: annotation2129080
version: 1
top level: BBa_K523022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129080/range2129080/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129080/range2129080/1
type: Range
persistent identity: range2129080
display id: range2129080
version: 1
direction: direction/forward
top level: BBa_K523022
start: 4038
end: 4067
orientation: inline

crtE
type: SequenceAnnotation
persistent identity: annotation2129070
display id: annotation2129070
version: 1
top level: BBa_K523022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523022/annotation2129070/range2129070/1

https://synbiohub.org/public/igem/BBa_K523022/annotation2129070/range2129070/1
type: Range
persistent identity: range2129070
display id: range2129070
version: 1
direction: direction/forward
top level: BBa_K523022
start: 623
end: 1558
orientation: inline

BBa_K523023
type: ComponentDefinition
persistent identity: BBa K523023
display id: BBa_K523023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlacLacZ + EYFP
creator: Sylvia Ispasanie, Mun Ching Lee
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the lac promoter driving Enhanced Yellow Fluorescent Protein. It was made as a test of the BioSandwich protocol of Edinburgh 2011.
mutable notes: N/A.
mutable provenance: BioSandwich Assembly of K523000 and K523012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Plac - LacZ
sequence annotation: -35
sequence annotation: Spacer
sequence annotation: TAATAA
sequence annotation: -10
sequence annotation: rbs
sequence annotation: GCA to GTA
sequence annotation: LacZ
sequence annotation: EYFP
sequence: https://synbiohub.org/public/igem/BBa_K523023_sequence/1

Plac - LacZ
type: SequenceAnnotation
persistent identity: annotation2129002
display id: annotation2129002
version: 1
top level: BBa_K523023
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129002/range2129002/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129002/range2129002/1
type: Range
persistent identity: range2129002
display id: range2129002
version: 1
direction: direction/forward
top level: BBa_K523023
start: 38
end: 589
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2129003
display id: annotation2129003
version: 1
top level: BBa_K523023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129003/range2129003/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129003/range2129003/1
type: Range
persistent identity: range2129003
display id: range2129003
version: 1
direction: direction/forward
top level: BBa_K523023
start: 286
end: 291
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2129007
display id: annotation2129007
version: 1
top level: BBa_K523023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129007/range2129007/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129007/range2129007/1
type: Range
persistent identity: range2129007
display id: range2129007
version: 1
direction: direction/forward
top level: BBa_K523023
start: 597
end: 625
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2129010
display id: annotation2129010
version: 1
top level: BBa_K523023
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129010/range2129010/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129010/range2129010/1
type: Range
persistent identity: range2129010
display id: range2129010
version: 1
direction: direction/forward
top level: BBa_K523023
start: 1340
end: 1340
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2129004
display id: annotation2129004
version: 1
top level: BBa_K523023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129004/range2129004/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129004/range2129004/1
type: Range
persistent identity: range2129004
display id: range2129004
version: 1
direction: direction/forward
top level: BBa_K523023
start: 309
end: 314
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129011
display id: annotation2129011
version: 1
top level: BBa_K523023
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129011/range2129011/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129011/range2129011/1
type: Range
persistent identity: range2129011
display id: range2129011
version: 1
direction: direction/forward
top level: BBa_K523023
start: 348
end: 351
orientation: inline

GCA to GTA
type: SequenceAnnotation
persistent identity: annotation2129006
display id: annotation2129006
version: 1
top level: BBa_K523023
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129006/range2129006/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129006/range2129006/1
type: Range
persistent identity: range2129006
display id: range2129006
version: 1
direction: direction/forward
top level: BBa_K523023
start: 447
end: 447
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2129001
display id: annotation2129001
version: 1
top level: BBa_K523023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129001/range2129001/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129001/range2129001/1
type: Range
persistent identity: range2129001
display id: range2129001
version: 1
direction: direction/forward
top level: BBa_K523023
start: 1
end: 32
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2129005
display id: annotation2129005
version: 1
top level: BBa_K523023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129005/range2129005/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129005/range2129005/1
type: Range
persistent identity: range2129005
display id: range2129005
version: 1
direction: direction/forward
top level: BBa_K523023
start: 359
end: 589
orientation: inline

EYFP
type: SequenceAnnotation
persistent identity: annotation2129009
display id: annotation2129009
version: 1
top level: BBa_K523023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129009/range2129009/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129009/range2129009/1
type: Range
persistent identity: range2129009
display id: range2129009
version: 1
direction: direction/forward
top level: BBa_K523023
start: 623
end: 1345
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129008
display id: annotation2129008
version: 1
top level: BBa_K523023
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523023/annotation2129008/range2129008/1

https://synbiohub.org/public/igem/BBa_K523023/annotation2129008/range2129008/1
type: Range
persistent identity: range2129008
display id: range2129008
version: 1
direction: direction/forward
top level: BBa_K523023
start: 611
end: 614
orientation: inline

BBa_K823011
type: ComponentDefinition
persistent identity: BBa K823011
display id: BBa_K823011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23114
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:50:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23114 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23114
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823011_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2182537
display id: annotation2182537
version: 1
top level: BBa_K823011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1
type: Range
persistent identity: range2182537
display id: range2182537
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182538
display id: annotation2182538
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1
type: Range
persistent identity: range2182538
display id: range2182538
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182539
display id: annotation2182539
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1
type: Range
persistent identity: range2182539
display id: range2182539
version: 1
direction: direction/forward
top level: BBa_K823011
start: 24
end: 29
orientation: inline

BBa_K863200
type: ComponentDefinition
persistent identity: BBa K863200
display id: BBa_K863200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 5' UTR site of alcohol oxidase 1 gene (aox1)
creator: Julia Schirmacher
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 5' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
mutable notes: coming soon
mutable provenance: K. pastoris
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863200_sequence/1

lytCFLAG
type: ComponentDefinition
persistent identity: BBa K733008
display id: BBa_K733008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lytC + linker + FLAG???
creator: CARIM, Sean; LAM Ka Shing; MOK Ka Chun; SHI, Tianxing
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FLAGTM peptide
sequence annotation: double stop codon
sequence annotation: lytC 1-954bp coding region
sequence annotation: helical linker
sequence: https://synbiohub.org/public/igem/BBa_K733008_sequence/1

FLAGTM peptide
type: SequenceAnnotation
persistent identity: annotation2194093
display id: annotation2194093
version: 1
top level: lytCFLAG
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K733008/annotation2194093/range2194093/1

https://synbiohub.org/public/igem/BBa_K733008/annotation2194093/range2194093/1
type: Range
persistent identity: range2194093
display id: range2194093
version: 1
direction: direction/forward
top level: lytCFLAG
start: 1033
end: 1056
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2194094
display id: annotation2194094
version: 1
top level: lytCFLAG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K733008/annotation2194094/range2194094/1

https://synbiohub.org/public/igem/BBa_K733008/annotation2194094/range2194094/1
type: Range
persistent identity: range2194094
display id: range2194094
version: 1
direction: direction/forward
top level: lytCFLAG
start: 1057
end: 1062
orientation: inline

lytC 1-954bp coding region
type: SequenceAnnotation
persistent identity: annotation2194091
display id: annotation2194091
version: 1
top level: lytCFLAG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K733008/annotation2194091/range2194091/1

https://synbiohub.org/public/igem/BBa_K733008/annotation2194091/range2194091/1
type: Range
persistent identity: range2194091
display id: range2194091
version: 1
direction: direction/forward
top level: lytCFLAG
start: 1
end: 954
orientation: inline

helical linker
type: SequenceAnnotation
persistent identity: annotation2194092
display id: annotation2194092
version: 1
top level: lytCFLAG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K733008/annotation2194092/range2194092/1

https://synbiohub.org/public/igem/BBa_K733008/annotation2194092/range2194092/1
type: Range
persistent identity: range2194092
display id: range2194092
version: 1
direction: direction/forward
top level: lytCFLAG
start: 955
end: 1032
orientation: inline

BBa_K575044
type: ComponentDefinition
persistent identity: BBa K575044
display id: BBa_K575044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Genomic LasP. producing GFP along with constiutivley producing LasR
mutable notes: no
mutable provenance: form genome and parts registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575040
component: BBa_K575004
sequence annotation: BBa_K575004
sequence annotation: BBa_K575040
sequence: https://synbiohub.org/public/igem/BBa_K575044_sequence/1

BBa_K575040
type: Component
persistent identity: component2138526
display id: component2138526
version: 1
top level: BBa_K575044
access: public
definition: BBa_K575040

BBa_K575004
type: Component
persistent identity: component2138532
display id: component2138532
version: 1
top level: BBa_K575044
access: public
definition: https://synbiohub.org/public/igem/BBa_K575004/1

BBa_K575004
type: SequenceAnnotation
persistent identity: annotation2138532
display id: annotation2138532
version: 1
top level: BBa_K575044
location: https://synbiohub.org/public/igem/BBa_K575044/annotation2138532/range2138532/1
component: BBa_K575004

https://synbiohub.org/public/igem/BBa_K575044/annotation2138532/range2138532/1
type: Range
persistent identity: range2138532
display id: range2138532
version: 1
direction: direction/reverse
top level: BBa_K575044
start: 1556
end: 2342
orientation: inline

BBa_K575040
type: SequenceAnnotation
persistent identity: annotation2138526
display id: annotation2138526
version: 1
top level: BBa_K575044
location: https://synbiohub.org/public/igem/BBa_K575044/annotation2138526/range2138526/1
component: BBa_K575040

https://synbiohub.org/public/igem/BBa_K575044/annotation2138526/range2138526/1
type: Range
persistent identity: range2138526
display id: range2138526
version: 1
direction: direction/reverse
top level: BBa_K575044
start: 1
end: 1547
orientation: inline

BBa_K554003
type: ComponentDefinition
persistent identity: BBa K554003
display id: BBa_K554003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SoxR
creator: UNICAMP EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: SoxR is ...
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: RBS
sequence annotation: SoxR
sequence: https://synbiohub.org/public/igem/BBa_K554003_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2136872
display id: annotation2136872
version: 1
top level: BBa_K554003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K554003/annotation2136872/range2136872/1

https://synbiohub.org/public/igem/BBa_K554003/annotation2136872/range2136872/1
type: Range
persistent identity: range2136872
display id: range2136872
version: 1
direction: direction/forward
top level: BBa_K554003
start: 478
end: 483
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136855
display id: annotation2136855
version: 1
top level: BBa_K554003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K554003/annotation2136855/range2136855/1

https://synbiohub.org/public/igem/BBa_K554003/annotation2136855/range2136855/1
type: Range
persistent identity: range2136855
display id: range2136855
version: 1
direction: direction/forward
top level: BBa_K554003
start: 1
end: 15
orientation: inline

SoxR
type: SequenceAnnotation
persistent identity: annotation2136870
display id: annotation2136870
version: 1
top level: BBa_K554003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K554003/annotation2136870/range2136870/1

https://synbiohub.org/public/igem/BBa_K554003/annotation2136870/range2136870/1
type: Range
persistent identity: range2136870
display id: range2136870
version: 1
direction: direction/forward
top level: BBa_K554003
start: 16
end: 477
orientation: inline

BBa_K592025
type: ComponentDefinition
persistent identity: BBa K592025
display id: BBa_K592025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-amilCP
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Blue chromoprotein amilCP ( BBa_K592009 ) with B0034 RBS. One can add any promoter upstream of this gene.
mutable notes: amilCP was made BioBrick-compatible after removal of two illegal restriction sites.
mutable provenance: amilCP is from Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592009
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K592009
sequence: https://synbiohub.org/public/igem/BBa_K592025_sequence/1

BBa_K592009
type: Component
persistent identity: component2219033
display id: component2219033
version: 1
top level: BBa_K592025
access: public
definition: amilCP

BBa_B0034
type: Component
persistent identity: component2219031
display id: component2219031
version: 1
top level: BBa_K592025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219031
display id: annotation2219031
version: 1
top level: BBa_K592025
location: https://synbiohub.org/public/igem/BBa_K592025/annotation2219031/range2219031/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592025/annotation2219031/range2219031/1
type: Range
persistent identity: range2219031
display id: range2219031
version: 1
direction: direction/reverse
top level: BBa_K592025
start: 1
end: 12
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2219033
display id: annotation2219033
version: 1
top level: BBa_K592025
location: https://synbiohub.org/public/igem/BBa_K592025/annotation2219033/range2219033/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K592025/annotation2219033/range2219033/1
type: Range
persistent identity: range2219033
display id: range2219033
version: 1
direction: direction/reverse
top level: BBa_K592025
start: 19
end: 687
orientation: inline

BBa_K575038
type: ComponentDefinition
persistent identity: BBa K575038
display id: BBa_K575038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP + Constitutive Promoter + RBS (B0030) + RhlR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of RhlR combined with receptor with RhlP.
mutable notes: put the constitutive promoter after the production of RhlR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575015
component: BBa_K575006
sequence annotation: BBa_K575015
sequence annotation: BBa_K575006
sequence: https://synbiohub.org/public/igem/BBa_K575038_sequence/1

BBa_K575015
type: Component
persistent identity: component2264223
display id: component2264223
version: 1
top level: BBa_K575038
access: public
definition: BBa_K575015

BBa_K575006
type: Component
persistent identity: component2264229
display id: component2264229
version: 1
top level: BBa_K575038
access: public
definition: https://synbiohub.org/public/igem/BBa_K575006/1

BBa_K575015
type: SequenceAnnotation
persistent identity: annotation2264223
display id: annotation2264223
version: 1
top level: BBa_K575038
location: https://synbiohub.org/public/igem/BBa_K575038/annotation2264223/range2264223/1
component: BBa_K575015

https://synbiohub.org/public/igem/BBa_K575038/annotation2264223/range2264223/1
type: Range
persistent identity: range2264223
display id: range2264223
version: 1
direction: direction/reverse
top level: BBa_K575038
start: 1
end: 785
orientation: inline

BBa_K575006
type: SequenceAnnotation
persistent identity: annotation2264229
display id: annotation2264229
version: 1
top level: BBa_K575038
location: https://synbiohub.org/public/igem/BBa_K575038/annotation2264229/range2264229/1
component: BBa_K575006

https://synbiohub.org/public/igem/BBa_K575038/annotation2264229/range2264229/1
type: Range
persistent identity: range2264229
display id: range2264229
version: 1
direction: direction/reverse
top level: BBa_K575038
start: 794
end: 1586
orientation: inline

BBa_K598012
type: ComponentDefinition
persistent identity: BBa K598012
display id: BBa_K598012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015
creator: Yangyang ZHAO, Zhenrun ZHANG, Yan GONG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 3541
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a basic part that contains a promoter, a ribozyme sequence numbered 1.20, a GFP part named E0040, a terminator part named B0015.
mutable notes: Use it by stantard assembly.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: pBAD
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: RBS
sequence annotation: eGFP
sequence annotation: TPP Up-regulate Hammerhead Ribozyme 1.20
sequence: https://synbiohub.org/public/igem/BBa_K598012_sequence/1

pBAD
type: SequenceAnnotation
persistent identity: annotation2144566
display id: annotation2144566
version: 1
top level: BBa_K598012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598012/annotation2144566/range2144566/1

https://synbiohub.org/public/igem/BBa_K598012/annotation2144566/range2144566/1
type: Range
persistent identity: range2144566
display id: range2144566
version: 1
direction: direction/forward
top level: BBa_K598012
start: 1
end: 130
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2155441
display id: annotation2155441
version: 1
top level: BBa_K598012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598012/annotation2155441/range2155441/1

https://synbiohub.org/public/igem/BBa_K598012/annotation2155441/range2155441/1
type: Range
persistent identity: range2155441
display id: range2155441
version: 1
direction: direction/forward
top level: BBa_K598012
start: 288
end: 1007
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2155442
display id: annotation2155442
version: 1
top level: BBa_K598012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598012/annotation2155442/range2155442/1

https://synbiohub.org/public/igem/BBa_K598012/annotation2155442/range2155442/1
type: Range
persistent identity: range2155442
display id: range2155442
version: 1
direction: direction/forward
top level: BBa_K598012
start: 1016
end: 1144
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2152714
display id: annotation2152714
version: 1
top level: BBa_K598012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598012/annotation2152714/range2152714/1

https://synbiohub.org/public/igem/BBa_K598012/annotation2152714/range2152714/1
type: Range
persistent identity: range2152714
display id: range2152714
version: 1
direction: direction/forward
top level: BBa_K598012
start: 275
end: 282
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152715
display id: annotation2152715
version: 1
top level: BBa_K598012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598012/annotation2152715/range2152715/1

https://synbiohub.org/public/igem/BBa_K598012/annotation2152715/range2152715/1
type: Range
persistent identity: range2152715
display id: range2152715
version: 1
direction: direction/forward
top level: BBa_K598012
start: 288
end: 1007
orientation: inline

TPP Up-regulate Hammerhead Ribozyme 1.20
type: SequenceAnnotation
persistent identity: annotation2152716
display id: annotation2152716
version: 1
top level: BBa_K598012
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598012/annotation2152716/range2152716/1

https://synbiohub.org/public/igem/BBa_K598012/annotation2152716/range2152716/1
type: Range
persistent identity: range2152716
display id: range2152716
version: 1
direction: direction/forward
top level: BBa_K598012
start: 137
end: 287
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa K525998
display id: BBa_K525998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter T7 and RBS
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter T7 and RBS
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: B0034
sequence annotation: T7 promoter
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525998_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2127788
display id: annotation2127788
version: 1
top level: pT7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2127788/range2127788/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2127788/range2127788/1
type: Range
persistent identity: range2127788
display id: range2127788
version: 1
direction: direction/forward
top level: pT7
start: 21
end: 32
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2129432
display id: annotation2129432
version: 1
top level: pT7
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2129432/range2129432/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2129432/range2129432/1
type: Range
persistent identity: range2129432
display id: range2129432
version: 1
direction: direction/forward
top level: pT7
start: 1
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129433
display id: annotation2129433
version: 1
top level: pT7
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2129433/range2129433/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2129433/range2129433/1
type: Range
persistent identity: range2129433
display id: range2129433
version: 1
direction: direction/forward
top level: pT7
start: 24
end: 27
orientation: inline

BBa_K398331
type: ComponentDefinition
persistent identity: BBa K398331
display id: BBa_K398331
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCaiF-B0032 measurement device
creator: Kira Schipper
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6322
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: p(Caif) facilitates the analysis of the transcriptional activity of the circuit responsive to Crp which is produced in the absence of glucose.
mutable notes: Nucleotide sequence optimized for expression in E.coli
mutable provenance: Genebank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_K398326
component: BBa_B0010
component: BBa_B0032
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_K398326
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K398331_sequence/1

BBa_E0040
type: Component
persistent identity: component2084229
display id: component2084229
version: 1
top level: BBa_K398331
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K398326
type: Component
persistent identity: component2084224
display id: component2084224
version: 1
top level: BBa_K398331
access: public
definition: pCaiF

BBa_B0010
type: Component
persistent identity: component2084230
display id: component2084230
version: 1
top level: BBa_K398331
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component2084226
display id: component2084226
version: 1
top level: BBa_K398331
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component2084232
display id: component2084232
version: 1
top level: BBa_K398331
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2084226
display id: annotation2084226
version: 1
top level: BBa_K398331
location: https://synbiohub.org/public/igem/BBa_K398331/annotation2084226/range2084226/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K398331/annotation2084226/range2084226/1
type: Range
persistent identity: range2084226
display id: range2084226
version: 1
direction: direction/forward
top level: BBa_K398331
start: 60
end: 72
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2084230
display id: annotation2084230
version: 1
top level: BBa_K398331
location: https://synbiohub.org/public/igem/BBa_K398331/annotation2084230/range2084230/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K398331/annotation2084230/range2084230/1
type: Range
persistent identity: range2084230
display id: range2084230
version: 1
direction: direction/forward
top level: BBa_K398331
start: 807
end: 886
orientation: inline

BBa_K398326
type: SequenceAnnotation
persistent identity: annotation2084224
display id: annotation2084224
version: 1
top level: BBa_K398331
location: https://synbiohub.org/public/igem/BBa_K398331/annotation2084224/range2084224/1
component: BBa_K398326

https://synbiohub.org/public/igem/BBa_K398331/annotation2084224/range2084224/1
type: Range
persistent identity: range2084224
display id: range2084224
version: 1
direction: direction/reverse
top level: BBa_K398331
start: 1
end: 51
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2084232
display id: annotation2084232
version: 1
top level: BBa_K398331
location: https://synbiohub.org/public/igem/BBa_K398331/annotation2084232/range2084232/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K398331/annotation2084232/range2084232/1
type: Range
persistent identity: range2084232
display id: range2084232
version: 1
direction: direction/forward
top level: BBa_K398331
start: 895
end: 935
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2084229
display id: annotation2084229
version: 1
top level: BBa_K398331
location: https://synbiohub.org/public/igem/BBa_K398331/annotation2084229/range2084229/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K398331/annotation2084229/range2084229/1
type: Range
persistent identity: range2084229
display id: range2084229
version: 1
direction: direction/reverse
top level: BBa_K398331
start: 79
end: 798
orientation: inline

BBa_K568006
type: ComponentDefinition
persistent identity: BBa K568006
display id: BBa_K568006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate synthetic part of optogenetical AND-Gate
creator: Team TU Munich 2011
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _736_
m user id: 0
owner id: 10022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Small parts of optogenetical AND-Gate.
mutable notes: Cloning of part was difficult due to small fragment sizes. Therefore whole part was synthesized.
mutable provenance: DNA was synthesized by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K228001
component: BBa_K238013
component: BBa_B0015
component: BBa_J44001
sequence annotation: BBa_J44001
sequence annotation: BBa_K238013
sequence annotation: BBa_B0015
sequence annotation: BBa_K228001
sequence: https://synbiohub.org/public/igem/BBa_K568006_sequence/1

BBa_K228001
type: Component
persistent identity: component2134822
display id: component2134822
version: 1
top level: BBa_K568006
access: public
definition: https://synbiohub.org/public/igem/BBa_K228001/1

BBa_K238013
type: Component
persistent identity: component2134836
display id: component2134836
version: 1
top level: BBa_K568006
access: public
definition: https://synbiohub.org/public/igem/BBa_K238013/1

BBa_B0015
type: Component
persistent identity: component2134829
display id: component2134829
version: 1
top level: BBa_K568006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J44001
type: Component
persistent identity: component2134838
display id: component2134838
version: 1
top level: BBa_K568006
access: public
definition: https://synbiohub.org/public/igem/BBa_J44001/1

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2134838
display id: annotation2134838
version: 1
top level: BBa_K568006
location: https://synbiohub.org/public/igem/BBa_K568006/annotation2134838/range2134838/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_K568006/annotation2134838/range2134838/1
type: Range
persistent identity: range2134838
display id: range2134838
version: 1
direction: direction/reverse
top level: BBa_K568006
start: 376
end: 390
orientation: inline

BBa_K238013
type: SequenceAnnotation
persistent identity: annotation2134836
display id: annotation2134836
version: 1
top level: BBa_K568006
location: https://synbiohub.org/public/igem/BBa_K568006/annotation2134836/range2134836/1
component: BBa_K238013

https://synbiohub.org/public/igem/BBa_K568006/annotation2134836/range2134836/1
type: Range
persistent identity: range2134836
display id: range2134836
version: 1
direction: direction/reverse
top level: BBa_K568006
start: 282
end: 367
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2134829
display id: annotation2134829
version: 1
top level: BBa_K568006
location: https://synbiohub.org/public/igem/BBa_K568006/annotation2134829/range2134829/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K568006/annotation2134829/range2134829/1
type: Range
persistent identity: range2134829
display id: range2134829
version: 1
direction: direction/reverse
top level: BBa_K568006
start: 145
end: 273
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2134822
display id: annotation2134822
version: 1
top level: BBa_K568006
location: https://synbiohub.org/public/igem/BBa_K568006/annotation2134822/range2134822/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K568006/annotation2134822/range2134822/1
type: Range
persistent identity: range2134822
display id: range2134822
version: 1
direction: direction/reverse
top level: BBa_K568006
start: 1
end: 136
orientation: inline

BBa_K598006
type: ComponentDefinition
persistent identity: BBa K598006
display id: BBa_K598006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline Responsive Riboswitch 1G1 with Engineered RBS
creator: Siyang HAO
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8816
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is a theophylline responsive riboswitch.
mutable notes: Get this part only by PCR, rather than enzyme digestion.
mutable provenance: By PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Theophylline responsive riboswitch Theo 1G1
sequence annotation: Engineered RBS
sequence: https://synbiohub.org/public/igem/BBa_K598006_sequence/1

Theophylline responsive riboswitch Theo 1G1
type: SequenceAnnotation
persistent identity: annotation2152995
display id: annotation2152995
version: 1
top level: BBa_K598006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598006/annotation2152995/range2152995/1

https://synbiohub.org/public/igem/BBa_K598006/annotation2152995/range2152995/1
type: Range
persistent identity: range2152995
display id: range2152995
version: 1
direction: direction/forward
top level: BBa_K598006
start: 1
end: 86
orientation: inline

Engineered RBS
type: SequenceAnnotation
persistent identity: annotation2150230
display id: annotation2150230
version: 1
top level: BBa_K598006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598006/annotation2150230/range2150230/1

https://synbiohub.org/public/igem/BBa_K598006/annotation2150230/range2150230/1
type: Range
persistent identity: range2150230
display id: range2150230
version: 1
direction: direction/forward
top level: BBa_K598006
start: 75
end: 81
orientation: inline

BBa_K523001
type: ComponentDefinition
persistent identity: BBa K523001
display id: BBa_K523001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + malS (E. coli periplasmic α-amylase)
creator: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the ''E. coli'' Amylase gene ''malS''. The part contains the native Ribosome Binding Site. The part was made using the strategy outlined in BBa_K523000 , and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
mutable notes: The part was made using the strategy outlined in BBa_K523000 , and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. The part was created from a lab ''E. coli'' strain using primers: *Forward: ACT AGATCT gaa atc gca gca ata agg act c **adds BglII site; starts upstream of gene to catch RBS; RFC10 compliant after replacement of BBa_K523000 in a normal BioBrick vector. *Reverse: ACT ACTAGT A TTA tta ctg ttg ccc tgc cca g **adds SpeI site; RFC10 compliant after insertion into vector; has double stop codon.
mutable provenance: The expected sequence can be seen using GenBank U00096.2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: TAATAA
sequence annotation: BglII
sequence annotation: ATG
sequence annotation: Native RBS
sequence annotation: malS
sequence annotation: Predicted (cleaved) signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K523001_sequence/1

TAATAA
type: SequenceAnnotation
persistent identity: annotation2123629
display id: annotation2123629
version: 1
top level: BBa_K523001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523001/annotation2123629/range2123629/1

https://synbiohub.org/public/igem/BBa_K523001/annotation2123629/range2123629/1
type: Range
persistent identity: range2123629
display id: range2123629
version: 1
direction: direction/forward
top level: BBa_K523001
start: 2062
end: 2062
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation2123625
display id: annotation2123625
version: 1
top level: BBa_K523001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523001/annotation2123625/range2123625/1

https://synbiohub.org/public/igem/BBa_K523001/annotation2123625/range2123625/1
type: Range
persistent identity: range2123625
display id: range2123625
version: 1
direction: direction/forward
top level: BBa_K523001
start: 1
end: 4
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2123627
display id: annotation2123627
version: 1
top level: BBa_K523001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523001/annotation2123627/range2123627/1

https://synbiohub.org/public/igem/BBa_K523001/annotation2123627/range2123627/1
type: Range
persistent identity: range2123627
display id: range2123627
version: 1
direction: direction/forward
top level: BBa_K523001
start: 34
end: 34
orientation: inline

Native RBS
type: SequenceAnnotation
persistent identity: annotation2123626
display id: annotation2123626
version: 1
top level: BBa_K523001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523001/annotation2123626/range2123626/1

https://synbiohub.org/public/igem/BBa_K523001/annotation2123626/range2123626/1
type: Range
persistent identity: range2123626
display id: range2123626
version: 1
direction: direction/forward
top level: BBa_K523001
start: 20
end: 23
orientation: inline

malS
type: SequenceAnnotation
persistent identity: annotation2123628
display id: annotation2123628
version: 1
top level: BBa_K523001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523001/annotation2123628/range2123628/1

https://synbiohub.org/public/igem/BBa_K523001/annotation2123628/range2123628/1
type: Range
persistent identity: range2123628
display id: range2123628
version: 1
direction: direction/forward
top level: BBa_K523001
start: 34
end: 2067
orientation: inline

Predicted (cleaved) signal peptide
type: SequenceAnnotation
persistent identity: annotation2123639
display id: annotation2123639
version: 1
top level: BBa_K523001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523001/annotation2123639/range2123639/1

https://synbiohub.org/public/igem/BBa_K523001/annotation2123639/range2123639/1
type: Range
persistent identity: range2123639
display id: range2123639
version: 1
direction: direction/forward
top level: BBa_K523001
start: 34
end: 84
orientation: inline

BBa_S05048
type: ComponentDefinition
persistent identity: BBa S05048
display id: BBa_S05048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:K748000
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:14:48Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K748000
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K748000
sequence: https://synbiohub.org/public/igem/BBa_S05048_sequence/1

BBa_K748000
type: Component
persistent identity: component2183412
display id: component2183412
version: 1
top level: BBa_S05048
access: public
definition: https://synbiohub.org/public/igem/BBa_K748000/1

BBa_B0034
type: Component
persistent identity: component2183411
display id: component2183411
version: 1
top level: BBa_S05048
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2183411
display id: annotation2183411
version: 1
top level: BBa_S05048
location: https://synbiohub.org/public/igem/BBa_S05048/annotation2183411/range2183411/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05048/annotation2183411/range2183411/1
type: Range
persistent identity: range2183411
display id: range2183411
version: 1
direction: direction/reverse
top level: BBa_S05048
start: 1
end: 12
orientation: inline

BBa_K748000
type: SequenceAnnotation
persistent identity: annotation2183412
display id: annotation2183412
version: 1
top level: BBa_S05048
location: https://synbiohub.org/public/igem/BBa_S05048/annotation2183412/range2183412/1
component: BBa_K748000

https://synbiohub.org/public/igem/BBa_S05048/annotation2183412/range2183412/1
type: Range
persistent identity: range2183412
display id: range2183412
version: 1
direction: direction/reverse
top level: BBa_S05048
start: 19
end: 648
orientation: inline

MpAAT1
type: ComponentDefinition
persistent identity: BBa K395602
display id: BBa_K395602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Apple fragrance generator
creator: Toshitaka Matsubara
created: 2010-10-03T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _505_
m user id: 0
owner id: 7030
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The apple odor generator (BBa_K395602) catalyzes the conversion of the precursor 2-methylbutanol and butanol to the odor 2-methybutyl acetate and butyl acetate that has a apple smell. The biosynthetic device is composed of two devices:odor enzyme generator (BBa_K395601) and a T7 terminator (BBa_K395600).
mutable notes: T7 promoter+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag+T7 terminator
mutable provenance: apple
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K395601
component: BBa_K395600
sequence annotation: BBa_K395600
sequence annotation: BBa_K395601
sequence: https://synbiohub.org/public/igem/BBa_K395602_sequence/1

BBa_K395601
type: Component
persistent identity: component2105807
display id: component2105807
version: 1
top level: MpAAT1
access: public
definition: https://synbiohub.org/public/igem/BBa_K395601/1

BBa_K395600
type: Component
persistent identity: component2105806
display id: component2105806
version: 1
top level: MpAAT1
access: public
definition: https://synbiohub.org/public/igem/BBa_K395600/1

BBa_K395600
type: SequenceAnnotation
persistent identity: annotation2105806
display id: annotation2105806
version: 1
top level: MpAAT1
location: https://synbiohub.org/public/igem/BBa_K395602/annotation2105806/range2105806/1
component: BBa_K395600

https://synbiohub.org/public/igem/BBa_K395602/annotation2105806/range2105806/1
type: Range
persistent identity: range2105806
display id: range2105806
version: 1
direction: direction/reverse
top level: MpAAT1
start: 1
end: 1757
orientation: inline

BBa_K395601
type: SequenceAnnotation
persistent identity: annotation2105807
display id: annotation2105807
version: 1
top level: MpAAT1
location: https://synbiohub.org/public/igem/BBa_K395602/annotation2105807/range2105807/1
component: BBa_K395601

https://synbiohub.org/public/igem/BBa_K395602/annotation2105807/range2105807/1
type: Range
persistent identity: range2105807
display id: range2105807
version: 1
direction: direction/reverse
top level: MpAAT1
start: 1766
end: 1813
orientation: inline

BBa_K733010
type: ComponentDefinition
persistent identity: BBa K733010
display id: BBa_K733010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptms+RBS+ydcD.
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K733003
sequence annotation: RBS
sequence annotation: ydcD
sequence annotation: BBa_K733001
sequence annotation: pTms
sequence: https://synbiohub.org/public/igem/BBa_K733010_sequence/1

BBa_K733003
type: SequenceAnnotation
persistent identity: annotation2193681
display id: annotation2193681
version: 1
top level: BBa_K733010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K733010/annotation2193681/range2193681/1

https://synbiohub.org/public/igem/BBa_K733010/annotation2193681/range2193681/1
type: Range
persistent identity: range2193681
display id: range2193681
version: 1
direction: direction/reverse
top level: BBa_K733010
start: 71
end: 371
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2193682
display id: annotation2193682
version: 1
top level: BBa_K733010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K733010/annotation2193682/range2193682/1

https://synbiohub.org/public/igem/BBa_K733010/annotation2193682/range2193682/1
type: Range
persistent identity: range2193682
display id: range2193682
version: 1
direction: direction/forward
top level: BBa_K733010
start: 71
end: 82
orientation: inline

ydcD
type: SequenceAnnotation
persistent identity: annotation2193703
display id: annotation2193703
version: 1
top level: BBa_K733010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K733010/annotation2193703/range2193703/1

https://synbiohub.org/public/igem/BBa_K733010/annotation2193703/range2193703/1
type: Range
persistent identity: range2193703
display id: range2193703
version: 1
direction: direction/forward
top level: BBa_K733010
start: 90
end: 371
orientation: inline

BBa_K733001
type: SequenceAnnotation
persistent identity: annotation2193680
display id: annotation2193680
version: 1
top level: BBa_K733010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K733010/annotation2193680/range2193680/1

https://synbiohub.org/public/igem/BBa_K733010/annotation2193680/range2193680/1
type: Range
persistent identity: range2193680
display id: range2193680
version: 1
direction: direction/reverse
top level: BBa_K733010
start: 1
end: 65
orientation: inline

pTms
type: SequenceAnnotation
persistent identity: annotation2193702
display id: annotation2193702
version: 1
top level: BBa_K733010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K733010/annotation2193702/range2193702/1

https://synbiohub.org/public/igem/BBa_K733010/annotation2193702/range2193702/1
type: Range
persistent identity: range2193702
display id: range2193702
version: 1
direction: direction/forward
top level: BBa_K733010
start: 1
end: 65
orientation: inline

BBa_K381001
type: ComponentDefinition
persistent identity: BBa K381001
display id: BBa_K381001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitrate reporter: PyeaR - GFP composite
creator: Katharine Coyte
created: 2010-10-04T11:00:00Z
modified: 2015-05-08T01:12:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _487_
m user id: 0
owner id: 6940
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Nitrate and Nitrite sensitive promoter PyeaR with a GFP coding device and strong RBS to create a nitrate-sensitive system which signals through expression of GFP. Optimum use appears to be when sensing nitrate within a range of 0 - 20 mM
mutable notes: None
mutable provenance: Combination of two existing biobricks from the parts registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0012
component: BBa_K216005
component: BBa_B0030
component: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_K216005
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K381001_sequence/1

BBa_B0010
type: Component
persistent identity: component2082870
display id: component2082870
version: 1
top level: BBa_K381001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2082872
display id: component2082872
version: 1
top level: BBa_K381001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K216005
type: Component
persistent identity: component2082864
display id: component2082864
version: 1
top level: BBa_K381001
access: public
definition: https://synbiohub.org/public/igem/BBa_K216005/1

BBa_B0030
type: Component
persistent identity: component2082866
display id: component2082866
version: 1
top level: BBa_K381001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0040
type: Component
persistent identity: component2082869
display id: component2082869
version: 1
top level: BBa_K381001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2082872
display id: annotation2082872
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082872/range2082872/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K381001/annotation2082872/range2082872/1
type: Range
persistent identity: range2082872
display id: range2082872
version: 1
direction: direction/forward
top level: BBa_K381001
start: 946
end: 986
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2082866
display id: annotation2082866
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082866/range2082866/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K381001/annotation2082866/range2082866/1
type: Range
persistent identity: range2082866
display id: range2082866
version: 1
direction: direction/forward
top level: BBa_K381001
start: 109
end: 123
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2082869
display id: annotation2082869
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082869/range2082869/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K381001/annotation2082869/range2082869/1
type: Range
persistent identity: range2082869
display id: range2082869
version: 1
direction: direction/reverse
top level: BBa_K381001
start: 130
end: 849
orientation: inline

BBa_K216005
type: SequenceAnnotation
persistent identity: annotation2082864
display id: annotation2082864
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082864/range2082864/1
component: BBa_K216005

https://synbiohub.org/public/igem/BBa_K381001/annotation2082864/range2082864/1
type: Range
persistent identity: range2082864
display id: range2082864
version: 1
direction: direction/reverse
top level: BBa_K381001
start: 1
end: 100
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2082870
display id: annotation2082870
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082870/range2082870/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K381001/annotation2082870/range2082870/1
type: Range
persistent identity: range2082870
display id: range2082870
version: 1
direction: direction/forward
top level: BBa_K381001
start: 858
end: 937
orientation: inline

BBa_K823013
type: ComponentDefinition
persistent identity: BBa K823013
display id: BBa_K823013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23117
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23117 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23117
sequence: https://synbiohub.org/public/igem/BBa_K823013_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182544
display id: annotation2182544
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1
type: Range
persistent identity: range2182544
display id: range2182544
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182545
display id: annotation2182545
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1
type: Range
persistent identity: range2182545
display id: range2182545
version: 1
direction: direction/forward
top level: BBa_K823013
start: 24
end: 29
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2182543
display id: annotation2182543
version: 1
top level: BBa_K823013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1
type: Range
persistent identity: range2182543
display id: range2182543
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 35
orientation: inline

BBa_K517002
type: ComponentDefinition
persistent identity: BBa K517002
display id: BBa_K517002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alpha-pinene synthase
creator: Joe Ho
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _682_
m user id: 0
owner id: 9774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The (-)-a-pinene synthase converts geranyl pyrophosphate to 83.4%(-)-alpha-pinene, 12.6%(-)-beta-pinene, 2.1% Linalool, 1.0% (-)-beta-phellandrene, 0.4% Camphene, and 0.4% myrcene.
mutable notes: none available
mutable provenance: The (-)-a-pinene synthase (PsTPS-Pin) synthase is a monoterpene synthase isolated from Sitka spruce
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K517002_sequence/1

BBa_K864202
type: ComponentDefinition
persistent identity: BBa K864202
display id: BBa_K864202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MarR(G95S) super-repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: marR(G95S) is a super-repressor
mutable notes: marR(G95S) carries a G>A mutation at position 284 which gives a amino acid change G95S. Has been mutated from BBa_K864200.
mutable provenance: BBa_K864200
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G>A
sequence annotation: G95S
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K864202_sequence/1

G>A
type: SequenceAnnotation
persistent identity: annotation2197128
display id: annotation2197128
version: 1
top level: BBa_K864202
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197128/range2197128/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197128/range2197128/1
type: Range
persistent identity: range2197128
display id: range2197128
version: 1
direction: direction/forward
top level: BBa_K864202
start: 283
end: 283
orientation: inline

G95S
type: SequenceAnnotation
persistent identity: annotation2197127
display id: annotation2197127
version: 1
top level: BBa_K864202
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197127/range2197127/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197127/range2197127/1
type: Range
persistent identity: range2197127
display id: range2197127
version: 1
direction: direction/forward
top level: BBa_K864202
start: 283
end: 285
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2197126
display id: annotation2197126
version: 1
top level: BBa_K864202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197126/range2197126/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197126/range2197126/1
type: Range
persistent identity: range2197126
display id: range2197126
version: 1
direction: direction/forward
top level: BBa_K864202
start: 1
end: 438
orientation: inline

BBa_K861173
type: ComponentDefinition
persistent identity: BBa K861173
display id: BBa_K861173
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP generator controlled by PcstA (glucose-repressible promoter)
creator: Kuanwei Sheng, Xian Xia
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda cI is controlled by promoter PcstA,which can be activated by CRP-cAMP complex.therefore protein lambda cI will be expressed under low glucose concentration.This generator contains a RFP after the promoter as a reporter.
mutable notes: None
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K118011
sequence annotation: BBa_I13507
sequence annotation: BBa_K118011
sequence: https://synbiohub.org/public/igem/BBa_K861173_sequence/1

BBa_I13507
type: Component
persistent identity: component2193744
display id: component2193744
version: 1
top level: BBa_K861173
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K118011
type: Component
persistent identity: component2193732
display id: component2193732
version: 1
top level: BBa_K861173
access: public
definition: https://synbiohub.org/public/igem/BBa_K118011/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2193744
display id: annotation2193744
version: 1
top level: BBa_K861173
location: https://synbiohub.org/public/igem/BBa_K861173/annotation2193744/range2193744/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K861173/annotation2193744/range2193744/1
type: Range
persistent identity: range2193744
display id: range2193744
version: 1
direction: direction/reverse
top level: BBa_K861173
start: 140
end: 1000
orientation: inline

BBa_K118011
type: SequenceAnnotation
persistent identity: annotation2193732
display id: annotation2193732
version: 1
top level: BBa_K861173
location: https://synbiohub.org/public/igem/BBa_K861173/annotation2193732/range2193732/1
component: BBa_K118011

https://synbiohub.org/public/igem/BBa_K861173/annotation2193732/range2193732/1
type: Range
persistent identity: range2193732
display id: range2193732
version: 1
direction: direction/reverse
top level: BBa_K861173
start: 1
end: 131
orientation: inline

BBa_K592023
type: ComponentDefinition
persistent identity: BBa K592023
display id: BBa_K592023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032-BFP
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Blue Fluorescent Protein with B0032 RBS, this part can be added downstream to any promoter and then tested in FACS.
mutable notes: The BFP part ( BBa_K592100 ) was codon-optimized for expression in E.coli by DNA2.0
mutable provenance: The BFP part is BBa_K592100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592100
component: BBa_B0032
sequence annotation: BBa_K592100
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K592023_sequence/1

BBa_K592100
type: Component
persistent identity: component2136642
display id: component2136642
version: 1
top level: BBa_K592023
access: public
definition: BFP

BBa_B0032
type: Component
persistent identity: component2136639
display id: component2136639
version: 1
top level: BBa_K592023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K592100
type: SequenceAnnotation
persistent identity: annotation2136642
display id: annotation2136642
version: 1
top level: BBa_K592023
location: https://synbiohub.org/public/igem/BBa_K592023/annotation2136642/range2136642/1
component: BBa_K592100

https://synbiohub.org/public/igem/BBa_K592023/annotation2136642/range2136642/1
type: Range
persistent identity: range2136642
display id: range2136642
version: 1
direction: direction/reverse
top level: BBa_K592023
start: 20
end: 724
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2136639
display id: annotation2136639
version: 1
top level: BBa_K592023
location: https://synbiohub.org/public/igem/BBa_K592023/annotation2136639/range2136639/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K592023/annotation2136639/range2136639/1
type: Range
persistent identity: range2136639
display id: range2136639
version: 1
direction: direction/forward
top level: BBa_K592023
start: 1
end: 13
orientation: inline

BBa_K592020
type: ComponentDefinition
persistent identity: BBa K592020
display id: BBa_K592020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PFixK2-lambda C1-B0034-amilCP blue light sensor output
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FixK2 promoter is the downstream signaling promoter of YF1/FixJ blue light-sensing system. The YF1/FixJ system become "inactive" when illuminated by blue light, and "active" in darkness. Needless to say, a system that works in the opposite way with lights activating and darkness inactivating is more desirable. Therefore, an inverter was placed behind FixK2 promoter. When in dark, YF1 becomes phosphorylated and activates FixJ, its own RR. FixJ in turn binds to FixK2 promoter, allowing the transcription of the lambda C1, thus repressing the amilCP output. In light however, YF1/FixJ has no effect on FixK2 promoter, which in turn has near-zero activity. The lambda C1 concentration drops, eventually amilCP will be produced.
mutable notes: The Q04510 inverter contains B0015 double terminator which gives unspecific primer binding of VR. Touchdown PCR was required to amplify the sequence for screening.
mutable provenance: FixK2 promoter: Bradyrhizibium japonicum amilCP: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592006
component: BBa_Q04510
component: BBa_K592009
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_Q04510
sequence annotation: BBa_K592009
sequence annotation: BBa_K592006
sequence: https://synbiohub.org/public/igem/BBa_K592020_sequence/1

BBa_K592006
type: Component
persistent identity: component2226767
display id: component2226767
version: 1
top level: BBa_K592020
access: public
definition: BBa_K592006

BBa_Q04510
type: Component
persistent identity: component2226785
display id: component2226785
version: 1
top level: BBa_K592020
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04510/1

BBa_K592009
type: Component
persistent identity: component2226789
display id: component2226789
version: 1
top level: BBa_K592020
access: public
definition: amilCP

BBa_B0034
type: Component
persistent identity: component2226787
display id: component2226787
version: 1
top level: BBa_K592020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2226787
display id: annotation2226787
version: 1
top level: BBa_K592020
location: https://synbiohub.org/public/igem/BBa_K592020/annotation2226787/range2226787/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592020/annotation2226787/range2226787/1
type: Range
persistent identity: range2226787
display id: range2226787
version: 1
direction: direction/reverse
top level: BBa_K592020
start: 1254
end: 1265
orientation: inline

BBa_Q04510
type: SequenceAnnotation
persistent identity: annotation2226785
display id: annotation2226785
version: 1
top level: BBa_K592020
location: https://synbiohub.org/public/igem/BBa_K592020/annotation2226785/range2226785/1
component: BBa_Q04510

https://synbiohub.org/public/igem/BBa_K592020/annotation2226785/range2226785/1
type: Range
persistent identity: range2226785
display id: range2226785
version: 1
direction: direction/reverse
top level: BBa_K592020
start: 259
end: 1245
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2226789
display id: annotation2226789
version: 1
top level: BBa_K592020
location: https://synbiohub.org/public/igem/BBa_K592020/annotation2226789/range2226789/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K592020/annotation2226789/range2226789/1
type: Range
persistent identity: range2226789
display id: range2226789
version: 1
direction: direction/reverse
top level: BBa_K592020
start: 1272
end: 1940
orientation: inline

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2226767
display id: annotation2226767
version: 1
top level: BBa_K592020
location: https://synbiohub.org/public/igem/BBa_K592020/annotation2226767/range2226767/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_K592020/annotation2226767/range2226767/1
type: Range
persistent identity: range2226767
display id: range2226767
version: 1
direction: direction/reverse
top level: BBa_K592020
start: 1
end: 250
orientation: inline

BBa_K325218
type: ComponentDefinition
persistent identity: BBa K325218
display id: BBa_K325218
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Orange Firefly Luciferase (under pBAD) L. Cruciata (E. coli optimised)
creator: Anja Hohmann and Ben Reeve
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K325211
sequence annotation: BBa_K325211
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K325218_sequence/1

BBa_I0500
type: Component
persistent identity: component2101656
display id: component2101656
version: 1
top level: BBa_K325218
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K325211
type: Component
persistent identity: component2101660
display id: component2101660
version: 1
top level: BBa_K325218
access: public
definition: https://synbiohub.org/public/igem/BBa_K325211/1

BBa_K325211
type: SequenceAnnotation
persistent identity: annotation2101660
display id: annotation2101660
version: 1
top level: BBa_K325218
location: https://synbiohub.org/public/igem/BBa_K325218/annotation2101660/range2101660/1
component: BBa_K325211

https://synbiohub.org/public/igem/BBa_K325218/annotation2101660/range2101660/1
type: Range
persistent identity: range2101660
display id: range2101660
version: 1
direction: direction/reverse
top level: BBa_K325218
start: 1219
end: 2885
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2101656
display id: annotation2101656
version: 1
top level: BBa_K325218
location: https://synbiohub.org/public/igem/BBa_K325218/annotation2101656/range2101656/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K325218/annotation2101656/range2101656/1
type: Range
persistent identity: range2101656
display id: range2101656
version: 1
direction: direction/reverse
top level: BBa_K325218
start: 1
end: 1210
orientation: inline

BBa_K608006
type: ComponentDefinition
persistent identity: BBa K608006
display id: BBa_K608006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium Promoter , medium RBS
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: medium Promotor , medium RBS
mutable notes: composite
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0032
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K608006_sequence/1

BBa_B0032
type: Component
persistent identity: component2128661
display id: component2128661
version: 1
top level: BBa_K608006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J23110
type: Component
persistent identity: component2128659
display id: component2128659
version: 1
top level: BBa_K608006
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2128659
display id: annotation2128659
version: 1
top level: BBa_K608006
location: https://synbiohub.org/public/igem/BBa_K608006/annotation2128659/range2128659/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K608006/annotation2128659/range2128659/1
type: Range
persistent identity: range2128659
display id: range2128659
version: 1
direction: direction/reverse
top level: BBa_K608006
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128661
display id: annotation2128661
version: 1
top level: BBa_K608006
location: https://synbiohub.org/public/igem/BBa_K608006/annotation2128661/range2128661/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K608006/annotation2128661/range2128661/1
type: Range
persistent identity: range2128661
display id: range2128661
version: 1
direction: direction/forward
top level: BBa_K608006
start: 44
end: 56
orientation: inline

raiR
type: ComponentDefinition
persistent identity: BBa K594008
display id: BBa_K594008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a luxR-type regulator from Rhizobium etli CFN42
creator: Yu Xiaoyan
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _765_
m user id: 0
owner id: 8221
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: raiR, a necessary part of the rai system in Rhizobium etli CFN42, can combine with the 3-OH-C8-HSL and then bind the cassette in raiI promoter, activating the expression of raiI.
mutable notes: no
mutable provenance: The raiR is from the Rhizobium etli CFN42 from Nanjing Agriculture University.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: raiR
sequence: https://synbiohub.org/public/igem/BBa_K594008_sequence/1

raiR
type: SequenceAnnotation
persistent identity: annotation2148110
display id: annotation2148110
version: 1
top level: raiR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K594008/annotation2148110/range2148110/1

https://synbiohub.org/public/igem/BBa_K594008/annotation2148110/range2148110/1
type: Range
persistent identity: range2148110
display id: range2148110
version: 1
direction: direction/forward
top level: raiR
start: 1
end: 726
orientation: inline

BBa_K817022
type: ComponentDefinition
persistent identity: BBa K817022
display id: BBa_K817022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-SP2-Penetratin-Flag
creator: Jui-Tsen Yu
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1075_
m user id: 0
owner id: 13572
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS-SP2-xx
mutable notes: RBS-SP2-xx
mutable provenance: RBS-SP2-xx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0030
component: BBa_K817028
sequence annotation: BBa_B0030
sequence annotation: BBa_K817028
sequence: https://synbiohub.org/public/igem/BBa_K817022_sequence/1

BBa_B0030
type: Component
persistent identity: component2201532
display id: component2201532
version: 1
top level: BBa_K817022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K817028
type: Component
persistent identity: component2201537
display id: component2201537
version: 1
top level: BBa_K817022
access: public
definition: https://synbiohub.org/public/igem/BBa_K817028/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2201532
display id: annotation2201532
version: 1
top level: BBa_K817022
location: https://synbiohub.org/public/igem/BBa_K817022/annotation2201532/range2201532/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K817022/annotation2201532/range2201532/1
type: Range
persistent identity: range2201532
display id: range2201532
version: 1
direction: direction/forward
top level: BBa_K817022
start: 1
end: 15
orientation: inline

BBa_K817028
type: SequenceAnnotation
persistent identity: annotation2201537
display id: annotation2201537
version: 1
top level: BBa_K817022
location: https://synbiohub.org/public/igem/BBa_K817022/annotation2201537/range2201537/1
component: BBa_K817028

https://synbiohub.org/public/igem/BBa_K817022/annotation2201537/range2201537/1
type: Range
persistent identity: range2201537
display id: range2201537
version: 1
direction: direction/reverse
top level: BBa_K817022
start: 22
end: 162
orientation: inline

BBa_K611010
type: ComponentDefinition
persistent identity: BBa K611010
display id: BBa_K611010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Repressor Characterization Construct
creator: Nicholas Csicsery
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used to characterize mutants of BBa_C0012 (LacI). By adding a mutant of BBa_C0012 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.
mutable notes: There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3).
mutable provenance: Built from existing parts in the registry though BioBrick assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0040
component: BBa_R0010
component: BBa_B0015
component: BBa_I13453
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K611010_sequence/1

BBa_B0034
type: Component
persistent identity: component2133210
display id: component2133210
version: 1
top level: BBa_K611010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2133212
display id: component2133212
version: 1
top level: BBa_K611010
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component2133222
display id: component2133222
version: 1
top level: BBa_K611010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2133202
display id: component2133202
version: 1
top level: BBa_K611010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2133219
display id: component2133219
version: 1
top level: BBa_K611010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I13453
type: Component
persistent identity: component2133220
display id: component2133220
version: 1
top level: BBa_K611010
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2133202
display id: annotation2133202
version: 1
top level: BBa_K611010
location: https://synbiohub.org/public/igem/BBa_K611010/annotation2133202/range2133202/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K611010/annotation2133202/range2133202/1
type: Range
persistent identity: range2133202
display id: range2133202
version: 1
direction: direction/forward
top level: BBa_K611010
start: 1
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2133219
display id: annotation2133219
version: 1
top level: BBa_K611010
location: https://synbiohub.org/public/igem/BBa_K611010/annotation2133219/range2133219/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K611010/annotation2133219/range2133219/1
type: Range
persistent identity: range2133219
display id: range2133219
version: 1
direction: direction/reverse
top level: BBa_K611010
start: 955
end: 1083
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2133220
display id: annotation2133220
version: 1
top level: BBa_K611010
location: https://synbiohub.org/public/igem/BBa_K611010/annotation2133220/range2133220/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K611010/annotation2133220/range2133220/1
type: Range
persistent identity: range2133220
display id: range2133220
version: 1
direction: direction/reverse
top level: BBa_K611010
start: 1092
end: 1221
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133222
display id: annotation2133222
version: 1
top level: BBa_K611010
location: https://synbiohub.org/public/igem/BBa_K611010/annotation2133222/range2133222/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611010/annotation2133222/range2133222/1
type: Range
persistent identity: range2133222
display id: range2133222
version: 1
direction: direction/reverse
top level: BBa_K611010
start: 1230
end: 1241
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2133212
display id: annotation2133212
version: 1
top level: BBa_K611010
location: https://synbiohub.org/public/igem/BBa_K611010/annotation2133212/range2133212/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K611010/annotation2133212/range2133212/1
type: Range
persistent identity: range2133212
display id: range2133212
version: 1
direction: direction/reverse
top level: BBa_K611010
start: 227
end: 946
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133210
display id: annotation2133210
version: 1
top level: BBa_K611010
location: https://synbiohub.org/public/igem/BBa_K611010/annotation2133210/range2133210/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611010/annotation2133210/range2133210/1
type: Range
persistent identity: range2133210
display id: range2133210
version: 1
direction: direction/reverse
top level: BBa_K611010
start: 209
end: 220
orientation: inline

BBa_K523014
type: ComponentDefinition
persistent identity: BBa K523014
display id: BBa_K523014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac + LacZ + bglX
creator: Sylvia Ispasanie, Mun Ching Lee, Eugene Fletcher
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The ''E. coli'' periplasmic β-glucosidase gene bglX under the control of the lac promoter. The native ribosome binding site is present.
mutable notes: Standard Assembly.
mutable provenance: Composite of BBa_J33207 and BBa_K523002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J33207
component: BBa_K523002
sequence annotation: BBa_K523002
sequence annotation: BBa_J33207
sequence: https://synbiohub.org/public/igem/BBa_K523014_sequence/1

BBa_J33207
type: Component
persistent identity: component2126646
display id: component2126646
version: 1
top level: BBa_K523014
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_K523002
type: Component
persistent identity: component2126655
display id: component2126655
version: 1
top level: BBa_K523014
access: public
definition: BBa_K523002

BBa_K523002
type: SequenceAnnotation
persistent identity: annotation2126655
display id: annotation2126655
version: 1
top level: BBa_K523014
location: https://synbiohub.org/public/igem/BBa_K523014/annotation2126655/range2126655/1
component: BBa_K523002

https://synbiohub.org/public/igem/BBa_K523014/annotation2126655/range2126655/1
type: Range
persistent identity: range2126655
display id: range2126655
version: 1
direction: direction/reverse
top level: BBa_K523014
start: 609
end: 2946
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2126646
display id: annotation2126646
version: 1
top level: BBa_K523014
location: https://synbiohub.org/public/igem/BBa_K523014/annotation2126646/range2126646/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K523014/annotation2126646/range2126646/1
type: Range
persistent identity: range2126646
display id: range2126646
version: 1
direction: direction/reverse
top level: BBa_K523014
start: 1
end: 600
orientation: inline

BBa_S04607
type: ComponentDefinition
persistent identity: BBa S04607
display id: BBa_S04607
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011:C0077
creator: MASSON Cl??ment
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:14:42Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 8629
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0011
component: BBa_C0077
sequence annotation: BBa_C0077
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_S04607_sequence/1

BBa_R0011
type: Component
persistent identity: component2244657
display id: component2244657
version: 1
top level: BBa_S04607
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0077
type: Component
persistent identity: component2244665
display id: component2244665
version: 1
top level: BBa_S04607
access: public
definition: https://synbiohub.org/public/igem/BBa_C0077/1

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation2244665
display id: annotation2244665
version: 1
top level: BBa_S04607
location: https://synbiohub.org/public/igem/BBa_S04607/annotation2244665/range2244665/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_S04607/annotation2244665/range2244665/1
type: Range
persistent identity: range2244665
display id: range2244665
version: 1
direction: direction/reverse
top level: BBa_S04607
start: 62
end: 848
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2244657
display id: annotation2244657
version: 1
top level: BBa_S04607
location: https://synbiohub.org/public/igem/BBa_S04607/annotation2244657/range2244657/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_S04607/annotation2244657/range2244657/1
type: Range
persistent identity: range2244657
display id: range2244657
version: 1
direction: direction/forward
top level: BBa_S04607
start: 1
end: 54
orientation: inline

BBa_K542004
type: ComponentDefinition
persistent identity: BBa K542004
display id: BBa_K542004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI Regulated Lumazine Synthase with Transciptional Terminator and pBAD
creator: Anthony Vuong
created: 2011-09-22T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Made by assembling [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542001 BBa_K542001] with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542003 BBa_K542003]. Note that the pLacI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([http://partsregistry.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible. The microcompartment portion to the "Co-localization Construct".
mutable notes: The microcompartment portion to the "Co-localization Construct".
mutable provenance: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542001 BBa_K542001] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542003 BBa_K542003] were both assembled by the Lethbridge 2011 team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K542003
component: BBa_K542001
sequence annotation: BBa_K542003
sequence annotation: BBa_K542001
sequence: https://synbiohub.org/public/igem/BBa_K542004_sequence/1

BBa_K542003
type: Component
persistent identity: component2138635
display id: component2138635
version: 1
top level: BBa_K542004
access: public
definition: BBa_K542003

BBa_K542001
type: Component
persistent identity: component2138620
display id: component2138620
version: 1
top level: BBa_K542004
access: public
definition: https://synbiohub.org/public/igem/BBa_K542001/1

BBa_K542003
type: SequenceAnnotation
persistent identity: annotation2138635
display id: annotation2138635
version: 1
top level: BBa_K542004
location: https://synbiohub.org/public/igem/BBa_K542004/annotation2138635/range2138635/1
component: BBa_K542003

https://synbiohub.org/public/igem/BBa_K542004/annotation2138635/range2138635/1
type: Range
persistent identity: range2138635
display id: range2138635
version: 1
direction: direction/reverse
top level: BBa_K542004
start: 837
end: 1814
orientation: inline

BBa_K542001
type: SequenceAnnotation
persistent identity: annotation2138620
display id: annotation2138620
version: 1
top level: BBa_K542004
location: https://synbiohub.org/public/igem/BBa_K542004/annotation2138620/range2138620/1
component: BBa_K542001

https://synbiohub.org/public/igem/BBa_K542004/annotation2138620/range2138620/1
type: Range
persistent identity: range2138620
display id: range2138620
version: 1
direction: direction/reverse
top level: BBa_K542004
start: 1
end: 828
orientation: inline

BBa_K634001
type: ComponentDefinition
persistent identity: BBa K634001
display id: BBa_K634001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: exaE
creator: Kenneth O. Xu
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:57Z
discontinued: false
dominant: false
group u list: _809_
m user id: 0
owner id: 9393
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: exaE is a transcription factor that is activated by phosphorylation from exaD. It acts upon pexaA.
mutable notes: contains no biobrick cutsites
mutable provenance: de novo synthesis original amino acid sequence from pseudomonas aeruginosa.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rbs
sequence annotation: exaE
sequence: https://synbiohub.org/public/igem/BBa_K634001_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2129705
display id: annotation2129705
version: 1
top level: BBa_K634001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K634001/annotation2129705/range2129705/1

https://synbiohub.org/public/igem/BBa_K634001/annotation2129705/range2129705/1
type: Range
persistent identity: range2129705
display id: range2129705
version: 1
direction: direction/forward
top level: BBa_K634001
start: 1
end: 14
orientation: inline

exaE
type: SequenceAnnotation
persistent identity: annotation2129706
display id: annotation2129706
version: 1
top level: BBa_K634001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K634001/annotation2129706/range2129706/1

https://synbiohub.org/public/igem/BBa_K634001/annotation2129706/range2129706/1
type: Range
persistent identity: range2129706
display id: range2129706
version: 1
direction: direction/forward
top level: BBa_K634001
start: 15
end: 692
orientation: inline

BBa_K808013
type: ComponentDefinition
persistent identity: BBa K808013
display id: BBa_K808013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphA3: Catalyzes together with tphA2 TPA to DCD
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K808013_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2181847
display id: annotation2181847
version: 1
top level: BBa_K808013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808013/annotation2181847/range2181847/1

https://synbiohub.org/public/igem/BBa_K808013/annotation2181847/range2181847/1
type: Range
persistent identity: range2181847
display id: range2181847
version: 1
direction: direction/forward
top level: BBa_K808013
start: 1
end: 465
orientation: inline

BBa_K346002
type: ComponentDefinition
persistent identity: BBa K346002
display id: BBa_K346002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PmerT promoter (mercury-responsive)
creator: Qianzhu Wu & Mei Chen
created: 2010-10-11T11:00:00Z
modified: 2015-07-14T12:33:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 4411
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part was isolated from Tn21. When regulated by its cognate transcription factor MerR, it will represent a dose-response manner in response to the concentration of mercury.
mutable notes: It was divergently regulated by MerR
mutable provenance: TTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K346002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2085551
display id: annotation2085551
version: 1
top level: BBa_K346002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K346002/annotation2085551/range2085551/1

https://synbiohub.org/public/igem/BBa_K346002/annotation2085551/range2085551/1
type: Range
persistent identity: range2085551
display id: range2085551
version: 1
direction: direction/forward
top level: BBa_K346002
start: 1
end: 57
orientation: inline

otsB
type: ComponentDefinition
persistent identity: BBa K847061
display id: BBa_K847061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Escherichia coli'' osmoregulatory trehalose synthesis B (otsB)
creator: Rashmi Sharma, Vishesh Jain, Kendrick Wang
created: 2012-08-08T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1107_
m user id: 0
owner id: 11092
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tab
mutable notes: tba
mutable provenance: tba
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K847061_sequence/1

BBa_K624004
type: ComponentDefinition
persistent identity: BBa K624004
display id: BBa_K624004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ori+Pmsp1+rep (pYMB essentials)
creator: SHANG-JUI TSAI, CHUN-YU LAI
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _796_
m user id: 0
owner id: 6664
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: express in AMB-1
mutable notes: Express in AMB-1
mutable provenance: Express in AMB-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K624000
component: BBa_K624003
sequence annotation: BBa_K624003
sequence annotation: BBa_K624000
sequence: https://synbiohub.org/public/igem/BBa_K624004_sequence/1

BBa_K624000
type: Component
persistent identity: component2148120
display id: component2148120
version: 1
top level: BBa_K624004
access: public
definition: BBa_K624000

BBa_K624003
type: Component
persistent identity: component2148123
display id: component2148123
version: 1
top level: BBa_K624004
access: public
definition: BBa_K624003

BBa_K624003
type: SequenceAnnotation
persistent identity: annotation2148123
display id: annotation2148123
version: 1
top level: BBa_K624004
location: https://synbiohub.org/public/igem/BBa_K624004/annotation2148123/range2148123/1
component: BBa_K624003

https://synbiohub.org/public/igem/BBa_K624004/annotation2148123/range2148123/1
type: Range
persistent identity: range2148123
display id: range2148123
version: 1
direction: direction/reverse
top level: BBa_K624004
start: 286
end: 1142
orientation: inline

BBa_K624000
type: SequenceAnnotation
persistent identity: annotation2148120
display id: annotation2148120
version: 1
top level: BBa_K624004
location: https://synbiohub.org/public/igem/BBa_K624004/annotation2148120/range2148120/1
component: BBa_K624000

https://synbiohub.org/public/igem/BBa_K624004/annotation2148120/range2148120/1
type: Range
persistent identity: range2148120
display id: range2148120
version: 1
direction: direction/reverse
top level: BBa_K624004
start: 1
end: 277
orientation: inline

BBa_K541596
type: ComponentDefinition
persistent identity: BBa K541596
display id: BBa_K541596
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reflectin gene with J04500 promoter
creator: Fazilet Guler
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _708_
m user id: 0
owner id: 8463
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge- shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.
mutable notes: Optimized for E.coli
mutable provenance: Horseshoe crab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K541506
component: BBa_J04500
sequence annotation: BBa_K541506
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K541596_sequence/1

BBa_K541506
type: Component
persistent identity: component2137861
display id: component2137861
version: 1
top level: BBa_K541596
access: public
definition: BBa_K541506

BBa_J04500
type: Component
persistent identity: component2137859
display id: component2137859
version: 1
top level: BBa_K541596
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K541506
type: SequenceAnnotation
persistent identity: annotation2137861
display id: annotation2137861
version: 1
top level: BBa_K541596
location: https://synbiohub.org/public/igem/BBa_K541596/annotation2137861/range2137861/1
component: BBa_K541506

https://synbiohub.org/public/igem/BBa_K541596/annotation2137861/range2137861/1
type: Range
persistent identity: range2137861
display id: range2137861
version: 1
direction: direction/reverse
top level: BBa_K541596
start: 227
end: 1081
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2137859
display id: annotation2137859
version: 1
top level: BBa_K541596
location: https://synbiohub.org/public/igem/BBa_K541596/annotation2137859/range2137859/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K541596/annotation2137859/range2137859/1
type: Range
persistent identity: range2137859
display id: range2137859
version: 1
direction: direction/reverse
top level: BBa_K541596
start: 1
end: 220
orientation: inline

BBa_K864400
type: ComponentDefinition
persistent identity: BBa K864400
display id: BBa_K864400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac, trp & lac regulated promoter
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part BBa_K180000 [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
mutable notes: Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
mutable provenance: Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: lacO1 site
sequence annotation: Ptac
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K864400_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2197238
display id: annotation2197238
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1
type: Range
persistent identity: range2197238
display id: range2197238
version: 1
direction: direction/forward
top level: BBa_K864400
start: 29
end: 33
orientation: inline

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2197240
display id: annotation2197240
version: 1
top level: BBa_K864400
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1
type: Range
persistent identity: range2197240
display id: range2197240
version: 1
direction: direction/forward
top level: BBa_K864400
start: 36
end: 61
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2197236
display id: annotation2197236
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1
type: Range
persistent identity: range2197236
display id: range2197236
version: 1
direction: direction/forward
top level: BBa_K864400
start: 1
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2197237
display id: annotation2197237
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1
type: Range
persistent identity: range2197237
display id: range2197237
version: 1
direction: direction/forward
top level: BBa_K864400
start: 7
end: 12
orientation: inline

BBa_K575014
type: ComponentDefinition
persistent identity: BBa K575014
display id: BBa_K575014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0030) + RFP
creator: Kristin Palarz
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR/PAI2 inducable promoter with RBS (B0030) and RFP.
mutable notes: Need to ensure no leaky transcription.
mutable provenance: From BioBrick Parts Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0071
component: BBa_E1010
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_R0071
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K575014_sequence/1

BBa_R0071
type: Component
persistent identity: component2247813
display id: component2247813
version: 1
top level: BBa_K575014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_E1010
type: Component
persistent identity: component2247819
display id: component2247819
version: 1
top level: BBa_K575014
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0030
type: Component
persistent identity: component2247815
display id: component2247815
version: 1
top level: BBa_K575014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2247815
display id: annotation2247815
version: 1
top level: BBa_K575014
location: https://synbiohub.org/public/igem/BBa_K575014/annotation2247815/range2247815/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K575014/annotation2247815/range2247815/1
type: Range
persistent identity: range2247815
display id: range2247815
version: 1
direction: direction/forward
top level: BBa_K575014
start: 62
end: 76
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2247813
display id: annotation2247813
version: 1
top level: BBa_K575014
location: https://synbiohub.org/public/igem/BBa_K575014/annotation2247813/range2247813/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K575014/annotation2247813/range2247813/1
type: Range
persistent identity: range2247813
display id: range2247813
version: 1
direction: direction/reverse
top level: BBa_K575014
start: 1
end: 53
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247819
display id: annotation2247819
version: 1
top level: BBa_K575014
location: https://synbiohub.org/public/igem/BBa_K575014/annotation2247819/range2247819/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K575014/annotation2247819/range2247819/1
type: Range
persistent identity: range2247819
display id: range2247819
version: 1
direction: direction/reverse
top level: BBa_K575014
start: 83
end: 788
orientation: inline

BBa_K515100
type: ComponentDefinition
persistent identity: BBa K515100
display id: BBa_K515100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IAA biosynthetic genes under control of the Pveg2 promoter
creator: Atipat Patharagulpong
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The construct of IaaM and IaaH genes in PVEG plasmid
mutable notes: -
mutable provenance: genomics sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K515010
component: BBa_K515001
component: BBa_K515000
sequence annotation: BBa_K515001
sequence annotation: BBa_K515000
sequence annotation: BBa_K515010
sequence: https://synbiohub.org/public/igem/BBa_K515100_sequence/1

BBa_K515010
type: Component
persistent identity: component2133830
display id: component2133830
version: 1
top level: BBa_K515100
access: public
definition: https://synbiohub.org/public/igem/BBa_K515010/1

BBa_K515001
type: Component
persistent identity: component2133838
display id: component2133838
version: 1
top level: BBa_K515100
access: public
definition: https://synbiohub.org/public/igem/BBa_K515001/1

BBa_K515000
type: Component
persistent identity: component2133834
display id: component2133834
version: 1
top level: BBa_K515100
access: public
definition: https://synbiohub.org/public/igem/BBa_K515000/1

BBa_K515001
type: SequenceAnnotation
persistent identity: annotation2133838
display id: annotation2133838
version: 1
top level: BBa_K515100
location: https://synbiohub.org/public/igem/BBa_K515100/annotation2133838/range2133838/1
component: BBa_K515001

https://synbiohub.org/public/igem/BBa_K515100/annotation2133838/range2133838/1
type: Range
persistent identity: range2133838
display id: range2133838
version: 1
direction: direction/reverse
top level: BBa_K515100
start: 1784
end: 3165
orientation: inline

BBa_K515000
type: SequenceAnnotation
persistent identity: annotation2133834
display id: annotation2133834
version: 1
top level: BBa_K515100
location: https://synbiohub.org/public/igem/BBa_K515100/annotation2133834/range2133834/1
component: BBa_K515000

https://synbiohub.org/public/igem/BBa_K515100/annotation2133834/range2133834/1
type: Range
persistent identity: range2133834
display id: range2133834
version: 1
direction: direction/reverse
top level: BBa_K515100
start: 98
end: 1783
orientation: inline

BBa_K515010
type: SequenceAnnotation
persistent identity: annotation2133830
display id: annotation2133830
version: 1
top level: BBa_K515100
location: https://synbiohub.org/public/igem/BBa_K515100/annotation2133830/range2133830/1
component: BBa_K515010

https://synbiohub.org/public/igem/BBa_K515100/annotation2133830/range2133830/1
type: Range
persistent identity: range2133830
display id: range2133830
version: 1
direction: direction/reverse
top level: BBa_K515100
start: 1
end: 97
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter veg a constitutive promoter for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
direction: direction/forward
top level: Pveg
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
direction: direction/forward
top level: Pveg
start: 86
end: 91
orientation: inline

BBa_K325108
type: ComponentDefinition
persistent identity: BBa K325108
display id: BBa_K325108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EPIC Firefly Luciferase (under pBAD) P. Pyralis (E. coli optimised)
creator: Theo Sanderson and Paul Masset
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K325101
sequence annotation: BBa_I0500
sequence annotation: BBa_K325101
sequence: https://synbiohub.org/public/igem/BBa_K325108_sequence/1

BBa_I0500
type: Component
persistent identity: component2101610
display id: component2101610
version: 1
top level: BBa_K325108
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K325101
type: Component
persistent identity: component2101614
display id: component2101614
version: 1
top level: BBa_K325108
access: public
definition: https://synbiohub.org/public/igem/BBa_K325101/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2101610
display id: annotation2101610
version: 1
top level: BBa_K325108
location: https://synbiohub.org/public/igem/BBa_K325108/annotation2101610/range2101610/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K325108/annotation2101610/range2101610/1
type: Range
persistent identity: range2101610
display id: range2101610
version: 1
direction: direction/reverse
top level: BBa_K325108
start: 1
end: 1210
orientation: inline

BBa_K325101
type: SequenceAnnotation
persistent identity: annotation2101614
display id: annotation2101614
version: 1
top level: BBa_K325108
location: https://synbiohub.org/public/igem/BBa_K325108/annotation2101614/range2101614/1
component: BBa_K325101

https://synbiohub.org/public/igem/BBa_K325108/annotation2101614/range2101614/1
type: Range
persistent identity: range2101614
display id: range2101614
version: 1
direction: direction/reverse
top level: BBa_K325108
start: 1219
end: 2891
orientation: inline

BBa_K576005
type: ComponentDefinition
persistent identity: BBa K576005
display id: BBa_K576005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101-RBS-GFP1
creator: George Zarubin
created: 2011-06-19T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
group u list: _747_
m user id: 0
owner id: 7330
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The RFC53 HeadPart with promoter and RBS attached.
mutable notes: The format is RFC53 D-HeadPart, i.e. GGT 5' overhang.
mutable provenance: GFP1 by Davidson-Missouri 2007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K576001
component: BBa_B0034
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_K576001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K576005_sequence/1

BBa_K576001
type: Component
persistent identity: component2121643
display id: component2121643
version: 1
top level: BBa_K576005
access: public
definition: https://synbiohub.org/public/igem/BBa_K576001/1

BBa_B0034
type: Component
persistent identity: component2121640
display id: component2121640
version: 1
top level: BBa_K576005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23101
type: Component
persistent identity: component2121638
display id: component2121638
version: 1
top level: BBa_K576005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2121638
display id: annotation2121638
version: 1
top level: BBa_K576005
location: https://synbiohub.org/public/igem/BBa_K576005/annotation2121638/range2121638/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K576005/annotation2121638/range2121638/1
type: Range
persistent identity: range2121638
display id: range2121638
version: 1
direction: direction/reverse
top level: BBa_K576005
start: 1
end: 35
orientation: inline

BBa_K576001
type: SequenceAnnotation
persistent identity: annotation2121643
display id: annotation2121643
version: 1
top level: BBa_K576005
location: https://synbiohub.org/public/igem/BBa_K576005/annotation2121643/range2121643/1
component: BBa_K576001

https://synbiohub.org/public/igem/BBa_K576005/annotation2121643/range2121643/1
type: Range
persistent identity: range2121643
display id: range2121643
version: 1
direction: direction/reverse
top level: BBa_K576005
start: 62
end: 545
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2121640
display id: annotation2121640
version: 1
top level: BBa_K576005
location: https://synbiohub.org/public/igem/BBa_K576005/annotation2121640/range2121640/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K576005/annotation2121640/range2121640/1
type: Range
persistent identity: range2121640
display id: range2121640
version: 1
direction: direction/reverse
top level: BBa_K576005
start: 44
end: 55
orientation: inline

BBa_K592029
type: ComponentDefinition
persistent identity: BBa K592029
display id: BBa_K592029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcpcG2-B0034-BFP green light sensor output
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is designed to enable a quantitative analysis of the CcaS/CcaR green light-sensing system. The output is BFP ( BBa_K592100 ), which can be measured by e.g. FACS.
mutable notes: This part is similar to BBa_K592019 with BFP instead of amilGFP as output. While amilGFP is a good way to visualize, BFP is better for quantification.
mutable provenance: PcpcG2: Synechocystis sp. PCC6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K592100
component: BBa_K592003
component: BBa_B0034
sequence annotation: BBa_K592003
sequence annotation: BBa_B0034
sequence annotation: BBa_K592100
sequence: https://synbiohub.org/public/igem/BBa_K592029_sequence/1

BBa_K592100
type: Component
persistent identity: component2136925
display id: component2136925
version: 1
top level: BBa_K592029
access: public
definition: BFP

BBa_K592003
type: Component
persistent identity: component2136921
display id: component2136921
version: 1
top level: BBa_K592029
access: public
definition: https://synbiohub.org/public/igem/BBa_K592003/1

BBa_B0034
type: Component
persistent identity: component2136923
display id: component2136923
version: 1
top level: BBa_K592029
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592003
type: SequenceAnnotation
persistent identity: annotation2136921
display id: annotation2136921
version: 1
top level: BBa_K592029
location: https://synbiohub.org/public/igem/BBa_K592029/annotation2136921/range2136921/1
component: BBa_K592003

https://synbiohub.org/public/igem/BBa_K592029/annotation2136921/range2136921/1
type: Range
persistent identity: range2136921
display id: range2136921
version: 1
direction: direction/reverse
top level: BBa_K592029
start: 1
end: 238
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136923
display id: annotation2136923
version: 1
top level: BBa_K592029
location: https://synbiohub.org/public/igem/BBa_K592029/annotation2136923/range2136923/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592029/annotation2136923/range2136923/1
type: Range
persistent identity: range2136923
display id: range2136923
version: 1
direction: direction/reverse
top level: BBa_K592029
start: 247
end: 258
orientation: inline

BBa_K592100
type: SequenceAnnotation
persistent identity: annotation2136925
display id: annotation2136925
version: 1
top level: BBa_K592029
location: https://synbiohub.org/public/igem/BBa_K592029/annotation2136925/range2136925/1
component: BBa_K592100

https://synbiohub.org/public/igem/BBa_K592029/annotation2136925/range2136925/1
type: Range
persistent identity: range2136925
display id: range2136925
version: 1
direction: direction/reverse
top level: BBa_K592029
start: 265
end: 969
orientation: inline

BBa_K575008
type: ComponentDefinition
persistent identity: BBa K575008
display id: BBa_K575008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP
creator: Kristin Palarz
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The inducable Promoter for the LasR/PAI1 complex attached to RBS (B0030) and GFP.
mutable notes: Need to ensure no leaking transcription of GFP.
mutable provenance: From Biobrick parts registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_R0079
component: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_R0079
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K575008_sequence/1

BBa_E0040
type: Component
persistent identity: component2124822
display id: component2124822
version: 1
top level: BBa_K575008
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0079
type: Component
persistent identity: component2124817
display id: component2124817
version: 1
top level: BBa_K575008
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0030
type: Component
persistent identity: component2124819
display id: component2124819
version: 1
top level: BBa_K575008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2124822
display id: annotation2124822
version: 1
top level: BBa_K575008
location: https://synbiohub.org/public/igem/BBa_K575008/annotation2124822/range2124822/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K575008/annotation2124822/range2124822/1
type: Range
persistent identity: range2124822
display id: range2124822
version: 1
direction: direction/reverse
top level: BBa_K575008
start: 187
end: 906
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2124817
display id: annotation2124817
version: 1
top level: BBa_K575008
location: https://synbiohub.org/public/igem/BBa_K575008/annotation2124817/range2124817/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K575008/annotation2124817/range2124817/1
type: Range
persistent identity: range2124817
display id: range2124817
version: 1
direction: direction/reverse
top level: BBa_K575008
start: 1
end: 157
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2124819
display id: annotation2124819
version: 1
top level: BBa_K575008
location: https://synbiohub.org/public/igem/BBa_K575008/annotation2124819/range2124819/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K575008/annotation2124819/range2124819/1
type: Range
persistent identity: range2124819
display id: range2124819
version: 1
direction: direction/forward
top level: BBa_K575008
start: 166
end: 180
orientation: inline

BBa_K639003
type: ComponentDefinition
persistent identity: BBa K639003
display id: BBa_K639003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rrnB P1-LacI-pLac-mCherry plausible stress sensor
creator: Eirik Selsaas
created: 2011-08-25T11:00:00Z
modified: 2015-05-08T01:12:57Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _815_
m user id: 0
owner id: 9621
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The stringent response in bacteria is caused by amino-acid starvation, fatty acid limitation, iron limitation, heat shock and other stress conditions. As a response under these conditions, ''in vitro'' studies have suggested that the alarmone guanosine tetraphosphate (ppGpp) increase to modulate transcription to promote survival. The increase in ppGpp levels causes a redirection of transcription so that genes important for survival are favoured at the expense of those required for growth and proliferation [1]. So, could we use ppGpp as signal molecule to find out when cells are stressed? '''The solution''' Our system will be based on a promoter that is important for regulating growth and proliferation. At the moment we are trying to use the ''rrnB-p1'' promoter, which has been shown in earlier studies to be highly regulated by the ppGpp molecule. Hopefully the promoter will be down regulated enough by increased levels of ppGpp to turn the repressor ''lacI'' it controls completely off. The lacI represses a second promoter ''pLac'' that induces the production of red fluorescent protein (mCherry) and turns the cells red. Reference: http://www.sciencedirect.com/science/article/pii/S0966842X05000788 1 Magnusson, L. U., A. Farewell, et al. (2005). "ppGpp: a global regulator in Escherichia coli." Trends Microbiol 13(5): 236-242
mutable notes: None
mutable provenance: Made by combining BBa_K639002, BBa_K639000. BBa_K639002 is an improved version of BBa_K112118 made by PCR-amplification with BBa_K112118 as template. BBa_K639000 is made solely from existing registry parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K639000
component: BBa_K639002
sequence annotation: BBa_K639000
sequence annotation: BBa_K639002
sequence: https://synbiohub.org/public/igem/BBa_K639003_sequence/1

BBa_K639000
type: Component
persistent identity: component2289523
display id: component2289523
version: 1
top level: BBa_K639003
access: public
definition: https://synbiohub.org/public/igem/BBa_K639000/1

BBa_K639002
type: Component
persistent identity: component2289492
display id: component2289492
version: 1
top level: BBa_K639003
access: public
definition: https://synbiohub.org/public/igem/BBa_K639002/1

BBa_K639000
type: SequenceAnnotation
persistent identity: annotation2289523
display id: annotation2289523
version: 1
top level: BBa_K639003
location: https://synbiohub.org/public/igem/BBa_K639003/annotation2289523/range2289523/1
component: BBa_K639000

https://synbiohub.org/public/igem/BBa_K639003/annotation2289523/range2289523/1
type: Range
persistent identity: range2289523
display id: range2289523
version: 1
direction: direction/reverse
top level: BBa_K639003
start: 502
end: 2749
orientation: inline

BBa_K639002
type: SequenceAnnotation
persistent identity: annotation2289492
display id: annotation2289492
version: 1
top level: BBa_K639003
location: https://synbiohub.org/public/igem/BBa_K639003/annotation2289492/range2289492/1
component: BBa_K639002

https://synbiohub.org/public/igem/BBa_K639003/annotation2289492/range2289492/1
type: Range
persistent identity: range2289492
display id: range2289492
version: 1
direction: direction/reverse
top level: BBa_K639003
start: 1
end: 493
orientation: inline

BBa_K530019
type: ComponentDefinition
persistent identity: BBa K530019
display id: BBa_K530019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HHO1 Yeast UTR
creator: Daniel Wolozny
created: 2011-09-02T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _696_
m user id: 0
owner id: 8447
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: HHO1 yeast UTR is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
mutable notes: UTR was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the UTR.
mutable provenance: http://www.yeastgenome.org/cgi-bin/locus.fpl
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: HHO1 UTR
sequence: https://synbiohub.org/public/igem/BBa_K530019_sequence/1

HHO1 UTR
type: SequenceAnnotation
persistent identity: annotation2125815
display id: annotation2125815
version: 1
top level: BBa_K530019
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K530019/annotation2125815/range2125815/1

https://synbiohub.org/public/igem/BBa_K530019/annotation2125815/range2125815/1
type: Range
persistent identity: range2125815
display id: range2125815
version: 1
direction: direction/forward
top level: BBa_K530019
start: 1
end: 312
orientation: inline

BBa_K577895
type: ComponentDefinition
persistent identity: BBa K577895
display id: BBa_K577895
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressed RFP
creator: Kyle Jones
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _748_
m user id: 0
owner id: 8430
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A composite part containing the TetR gene, C0040, turned on by a constitutive promoter, I14033. The TetR represses the pTet promoter in front of the RFP gene. By addition of tetracycline or aTc, this repression is inhibited and the cells should fluoresce red.
mutable notes: The whole part is in the backbone of I14033. P0440, the TetR composite part was used and inserted into its respective promoter. The RBS+RFP+Terminator part came from the backbone J61102 and was inserted into the pTet promoter. The pTet+RFP part was then combined as a suffix insertion to the I14033+TetR backbone.
mutable provenance: Spring 2011 iGEM Kit Plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_B0015
component: BBa_B0012
component: BBa_C0040
component: BBa_I14033
component: BBa_R0040
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_I14033
sequence annotation: BBa_C0040
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K577895_sequence/1

BBa_B0034
type: Component
persistent identity: component2289632
display id: component2289632
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2289637
display id: component2289637
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0015
type: Component
persistent identity: component2289659
display id: component2289659
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2289649
display id: component2289649
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2289639
display id: component2289639
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component2289636
display id: component2289636
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_I14033
type: Component
persistent identity: component2289630
display id: component2289630
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_I14033/1

BBa_R0040
type: Component
persistent identity: component2289643
display id: component2289643
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E1010
type: Component
persistent identity: component2289652
display id: component2289652
version: 1
top level: BBa_K577895
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2289649
display id: annotation2289649
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289649/range2289649/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K577895/annotation2289649/range2289649/1
type: Range
persistent identity: range2289649
display id: range2289649
version: 1
direction: direction/reverse
top level: BBa_K577895
start: 957
end: 968
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2289637
display id: annotation2289637
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289637/range2289637/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K577895/annotation2289637/range2289637/1
type: Range
persistent identity: range2289637
display id: range2289637
version: 1
direction: direction/forward
top level: BBa_K577895
start: 758
end: 837
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2289643
display id: annotation2289643
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289643/range2289643/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K577895/annotation2289643/range2289643/1
type: Range
persistent identity: range2289643
display id: range2289643
version: 1
direction: direction/forward
top level: BBa_K577895
start: 895
end: 948
orientation: inline

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2289630
display id: annotation2289630
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289630/range2289630/1
component: BBa_I14033

https://synbiohub.org/public/igem/BBa_K577895/annotation2289630/range2289630/1
type: Range
persistent identity: range2289630
display id: range2289630
version: 1
direction: direction/reverse
top level: BBa_K577895
start: 1
end: 38
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2289632
display id: annotation2289632
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289632/range2289632/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K577895/annotation2289632/range2289632/1
type: Range
persistent identity: range2289632
display id: range2289632
version: 1
direction: direction/reverse
top level: BBa_K577895
start: 47
end: 58
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2289636
display id: annotation2289636
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289636/range2289636/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K577895/annotation2289636/range2289636/1
type: Range
persistent identity: range2289636
display id: range2289636
version: 1
direction: direction/reverse
top level: BBa_K577895
start: 65
end: 749
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2289652
display id: annotation2289652
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289652/range2289652/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K577895/annotation2289652/range2289652/1
type: Range
persistent identity: range2289652
display id: range2289652
version: 1
direction: direction/reverse
top level: BBa_K577895
start: 975
end: 1680
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2289659
display id: annotation2289659
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289659/range2289659/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K577895/annotation2289659/range2289659/1
type: Range
persistent identity: range2289659
display id: range2289659
version: 1
direction: direction/reverse
top level: BBa_K577895
start: 1689
end: 1817
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2289639
display id: annotation2289639
version: 1
top level: BBa_K577895
location: https://synbiohub.org/public/igem/BBa_K577895/annotation2289639/range2289639/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K577895/annotation2289639/range2289639/1
type: Range
persistent identity: range2289639
display id: range2289639
version: 1
direction: direction/forward
top level: BBa_K577895
start: 846
end: 886
orientation: inline

BBa_K733016
type: ComponentDefinition
persistent identity: BBa K733016
display id: BBa_K733016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg+RBS+ydjM+Bmp2+terminator
creator: Chris Yu Lai Cheong; LI Yiming
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K733014
sequence annotation: BBa_K733014
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K733016_sequence/1

BBa_B0015
type: Component
persistent identity: component2183826
display id: component2183826
version: 1
top level: BBa_K733016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K733014
type: Component
persistent identity: component2183819
display id: component2183819
version: 1
top level: BBa_K733016
access: public
definition: BBa_K733014

BBa_K733014
type: SequenceAnnotation
persistent identity: annotation2183819
display id: annotation2183819
version: 1
top level: BBa_K733016
location: https://synbiohub.org/public/igem/BBa_K733016/annotation2183819/range2183819/1
component: BBa_K733014

https://synbiohub.org/public/igem/BBa_K733016/annotation2183819/range2183819/1
type: Range
persistent identity: range2183819
display id: range2183819
version: 1
direction: direction/reverse
top level: BBa_K733016
start: 1
end: 578
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2183826
display id: annotation2183826
version: 1
top level: BBa_K733016
location: https://synbiohub.org/public/igem/BBa_K733016/annotation2183826/range2183826/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K733016/annotation2183826/range2183826/1
type: Range
persistent identity: range2183826
display id: range2183826
version: 1
direction: direction/reverse
top level: BBa_K733016
start: 587
end: 715
orientation: inline

BBa_K581012
type: ComponentDefinition
persistent identity: BBa K581012
display id: BBa_K581012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pqrr-rbs-T7ptag-terminator
creator: Weiyue Ji, Hanchi Lin, Dingqiao Wen, Handuo SHI
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _752_
m user id: 0
owner id: 8716
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: T7ptag behind pqrr.
mutable notes: We plan to use it in a logic gate with one input CAI-1, which can induce pqrr.
mutable provenance: The promoter qrr is from Vibrio cholera.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: B0030
sequence annotation: K228000(T7ptag)
sequence annotation: Pqrr
sequence: https://synbiohub.org/public/igem/BBa_K581012_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2154700
display id: annotation2154700
version: 1
top level: BBa_K581012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K581012/annotation2154700/range2154700/1

https://synbiohub.org/public/igem/BBa_K581012/annotation2154700/range2154700/1
type: Range
persistent identity: range2154700
display id: range2154700
version: 1
direction: direction/forward
top level: BBa_K581012
start: 2869
end: 2997
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation2154698
display id: annotation2154698
version: 1
top level: BBa_K581012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K581012/annotation2154698/range2154698/1

https://synbiohub.org/public/igem/BBa_K581012/annotation2154698/range2154698/1
type: Range
persistent identity: range2154698
display id: range2154698
version: 1
direction: direction/forward
top level: BBa_K581012
start: 167
end: 181
orientation: inline

K228000(T7ptag)
type: SequenceAnnotation
persistent identity: annotation2154699
display id: annotation2154699
version: 1
top level: BBa_K581012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K581012/annotation2154699/range2154699/1

https://synbiohub.org/public/igem/BBa_K581012/annotation2154699/range2154699/1
type: Range
persistent identity: range2154699
display id: range2154699
version: 1
direction: direction/forward
top level: BBa_K581012
start: 188
end: 2860
orientation: inline

Pqrr
type: SequenceAnnotation
persistent identity: annotation2154697
display id: annotation2154697
version: 1
top level: BBa_K581012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K581012/annotation2154697/range2154697/1

https://synbiohub.org/public/igem/BBa_K581012/annotation2154697/range2154697/1
type: Range
persistent identity: range2154697
display id: range2154697
version: 1
direction: direction/forward
top level: BBa_K581012
start: 1
end: 158
orientation: inline

BBa_K575039
type: ComponentDefinition
persistent identity: BBa K575039
display id: BBa_K575039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP + Constitutive Promoter + RBS (B0034) + RhlR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of RhlR combined with receptor with RhlP.
mutable notes: put the constitutive promoter after the production of RhlR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575015
component: BBa_K575007
sequence annotation: BBa_K575015
sequence annotation: BBa_K575007
sequence: https://synbiohub.org/public/igem/BBa_K575039_sequence/1

BBa_K575015
type: Component
persistent identity: component2264240
display id: component2264240
version: 1
top level: BBa_K575039
access: public
definition: BBa_K575015

BBa_K575007
type: Component
persistent identity: component2264245
display id: component2264245
version: 1
top level: BBa_K575039
access: public
definition: https://synbiohub.org/public/igem/BBa_K575007/1

BBa_K575015
type: SequenceAnnotation
persistent identity: annotation2264240
display id: annotation2264240
version: 1
top level: BBa_K575039
location: https://synbiohub.org/public/igem/BBa_K575039/annotation2264240/range2264240/1
component: BBa_K575015

https://synbiohub.org/public/igem/BBa_K575039/annotation2264240/range2264240/1
type: Range
persistent identity: range2264240
display id: range2264240
version: 1
direction: direction/reverse
top level: BBa_K575039
start: 1
end: 785
orientation: inline

BBa_K575007
type: SequenceAnnotation
persistent identity: annotation2264245
display id: annotation2264245
version: 1
top level: BBa_K575039
location: https://synbiohub.org/public/igem/BBa_K575039/annotation2264245/range2264245/1
component: BBa_K575007

https://synbiohub.org/public/igem/BBa_K575039/annotation2264245/range2264245/1
type: Range
persistent identity: range2264245
display id: range2264245
version: 1
direction: direction/reverse
top level: BBa_K575039
start: 794
end: 1583
orientation: inline

BBa_K523000
type: ComponentDefinition
persistent identity: BBa K523000
display id: BBa_K523000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac + lacZ; with BglII site
creator: Chris French, Allan Crossman, Sylvia Ispasanie
created: 2011-06-27T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is based on part BBa_J33207. Like that part, it encodes the lac promoter and the N-terminal 76 amino acid residues of LacZ, which produce a peptide that complements the lacZ-delta-M15 mutation in common lab strains of ''E. coli''. If grown on Xgal and IPTG, colonies with this part will be blue. The first four bases of the sequence add a BglII site (BglII sites have 6 bases, but the first 2 are provided by the standard BioBrick prefix). This enables an easy method of using PCR to create new BioBricks (e.g. from natural sources): the forward primer for the new BioBrick should have a BglII site added, and the reverse primer should have a SpeI site added. The PCR product and the plasmid containing this part can then both be digested with BglII and SpeI. After mixing and ligation, plasmids that have successfully gained the new BioBrick will be white, while those still containing the lacZ part will be blue (on Xgal+IPTG plates). One might ask: why not use the standard flanking BioBrick sites XbaI and SpeI for this procedure? The answer is that these produce compatible sticky ends and so, upon digestion, the new part will circularise.
mutable notes: Note that the BglII (agatct) site overlaps the BioBrick prefix. Since the prefix ends "ag", only "atct" is included in the BioBrick proper.
mutable provenance: Based on part BBa_J33207, which was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: LacI binding site
sequence annotation: ATG
sequence annotation: -10
sequence annotation: BglII
sequence annotation: -35
sequence annotation: CAP binding site
sequence annotation: TGA
sequence annotation: LacZ'
sequence: https://synbiohub.org/public/igem/BBa_K523000_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2122806
display id: annotation2122806
version: 1
top level: BBa_K523000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2122806/range2122806/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2122806/range2122806/1
type: Range
persistent identity: range2122806
display id: range2122806
version: 1
direction: direction/forward
top level: BBa_K523000
start: 315
end: 318
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2122811
display id: annotation2122811
version: 1
top level: BBa_K523000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2122811/range2122811/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2122811/range2122811/1
type: Range
persistent identity: range2122811
display id: range2122811
version: 1
direction: direction/forward
top level: BBa_K523000
start: 288
end: 308
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2123637
display id: annotation2123637
version: 1
top level: BBa_K523000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2123637/range2123637/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2123637/range2123637/1
type: Range
persistent identity: range2123637
display id: range2123637
version: 1
direction: direction/forward
top level: BBa_K523000
start: 326
end: 326
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2122810
display id: annotation2122810
version: 1
top level: BBa_K523000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2122810/range2122810/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2122810/range2122810/1
type: Range
persistent identity: range2122810
display id: range2122810
version: 1
direction: direction/forward
top level: BBa_K523000
start: 276
end: 281
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation2122805
display id: annotation2122805
version: 1
top level: BBa_K523000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2122805/range2122805/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2122805/range2122805/1
type: Range
persistent identity: range2122805
display id: range2122805
version: 1
direction: direction/forward
top level: BBa_K523000
start: 1
end: 4
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2122809
display id: annotation2122809
version: 1
top level: BBa_K523000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2122809/range2122809/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2122809/range2122809/1
type: Range
persistent identity: range2122809
display id: range2122809
version: 1
direction: direction/forward
top level: BBa_K523000
start: 253
end: 258
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2122808
display id: annotation2122808
version: 1
top level: BBa_K523000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2122808/range2122808/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2122808/range2122808/1
type: Range
persistent identity: range2122808
display id: range2122808
version: 1
direction: direction/forward
top level: BBa_K523000
start: 204
end: 241
orientation: inline

TGA
type: SequenceAnnotation
persistent identity: annotation2123638
display id: annotation2123638
version: 1
top level: BBa_K523000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2123638/range2123638/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2123638/range2123638/1
type: Range
persistent identity: range2123638
display id: range2123638
version: 1
direction: direction/forward
top level: BBa_K523000
start: 554
end: 554
orientation: inline

LacZ'
type: SequenceAnnotation
persistent identity: annotation2122807
display id: annotation2122807
version: 1
top level: BBa_K523000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523000/annotation2122807/range2122807/1

https://synbiohub.org/public/igem/BBa_K523000/annotation2122807/range2122807/1
type: Range
persistent identity: range2122807
display id: range2122807
version: 1
direction: direction/forward
top level: BBa_K523000
start: 326
end: 556
orientation: inline

BBa_K598009
type: ComponentDefinition
persistent identity: BBa K598009
display id: BBa_K598009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015
creator: Siyang HAO, Zhenrun ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8816
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is a theophylline responsive riboswitch containing an original RBS.
mutable notes: Change the promoter if required.
mutable provenance: By PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Native RBS
sequence annotation: Theo P1G1
sequence annotation: eGFP
sequence annotation: pBAD
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K598009_sequence/1

Native RBS
type: SequenceAnnotation
persistent identity: annotation2150235
display id: annotation2150235
version: 1
top level: BBa_K598009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598009/annotation2150235/range2150235/1

https://synbiohub.org/public/igem/BBa_K598009/annotation2150235/range2150235/1
type: Range
persistent identity: range2150235
display id: range2150235
version: 1
direction: direction/forward
top level: BBa_K598009
start: 213
end: 216
orientation: inline

Theo P1G1
type: SequenceAnnotation
persistent identity: annotation2150232
display id: annotation2150232
version: 1
top level: BBa_K598009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598009/annotation2150232/range2150232/1

https://synbiohub.org/public/igem/BBa_K598009/annotation2150232/range2150232/1
type: Range
persistent identity: range2150232
display id: range2150232
version: 1
direction: direction/forward
top level: BBa_K598009
start: 139
end: 225
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2150233
display id: annotation2150233
version: 1
top level: BBa_K598009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598009/annotation2150233/range2150233/1

https://synbiohub.org/public/igem/BBa_K598009/annotation2150233/range2150233/1
type: Range
persistent identity: range2150233
display id: range2150233
version: 1
direction: direction/forward
top level: BBa_K598009
start: 226
end: 945
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2150231
display id: annotation2150231
version: 1
top level: BBa_K598009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598009/annotation2150231/range2150231/1

https://synbiohub.org/public/igem/BBa_K598009/annotation2150231/range2150231/1
type: Range
persistent identity: range2150231
display id: range2150231
version: 1
direction: direction/forward
top level: BBa_K598009
start: 1
end: 130
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2150234
display id: annotation2150234
version: 1
top level: BBa_K598009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598009/annotation2150234/range2150234/1

https://synbiohub.org/public/igem/BBa_K598009/annotation2150234/range2150234/1
type: Range
persistent identity: range2150234
display id: range2150234
version: 1
direction: direction/forward
top level: BBa_K598009
start: 954
end: 1082
orientation: inline

BBa_K608004
type: ComponentDefinition
persistent identity: BBa K608004
display id: BBa_K608004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong Promoter , weak RBS
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: strong Promotor , weak RBS
mutable notes: composite
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23104
component: BBa_B0031
sequence annotation: BBa_J23104
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_K608004_sequence/1

BBa_J23104
type: Component
persistent identity: component2128653
display id: component2128653
version: 1
top level: BBa_K608004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23104/1

BBa_B0031
type: Component
persistent identity: component2128655
display id: component2128655
version: 1
top level: BBa_K608004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128653
display id: annotation2128653
version: 1
top level: BBa_K608004
location: https://synbiohub.org/public/igem/BBa_K608004/annotation2128653/range2128653/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608004/annotation2128653/range2128653/1
type: Range
persistent identity: range2128653
display id: range2128653
version: 1
direction: direction/reverse
top level: BBa_K608004
start: 1
end: 35
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2128655
display id: annotation2128655
version: 1
top level: BBa_K608004
location: https://synbiohub.org/public/igem/BBa_K608004/annotation2128655/range2128655/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K608004/annotation2128655/range2128655/1
type: Range
persistent identity: range2128655
display id: range2128655
version: 1
direction: direction/reverse
top level: BBa_K608004
start: 44
end: 57
orientation: inline

BBa_K530015
type: ComponentDefinition
persistent identity: BBa K530015
display id: BBa_K530015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FCY2 Yeast Promoter
creator: Daniel Wolozny
created: 2011-09-02T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
group u list: _696_
m user id: 0
owner id: 8447
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FCY2 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
mutable notes: Promoter was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the promoter.
mutable provenance: http://www.yeastgenome.org/cgi-bin/locus.fpl
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FCY2 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K530015_sequence/1

FCY2 Promoter
type: SequenceAnnotation
persistent identity: annotation2125810
display id: annotation2125810
version: 1
top level: BBa_K530015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K530015/annotation2125810/range2125810/1

https://synbiohub.org/public/igem/BBa_K530015/annotation2125810/range2125810/1
type: Range
persistent identity: range2125810
display id: range2125810
version: 1
direction: direction/forward
top level: BBa_K530015
start: 1
end: 901
orientation: inline

BBa_K864440
type: ComponentDefinition
persistent identity: BBa K864440
display id: BBa_K864440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Small RNA spot42
creator: Hampus Elofsson, Sabri Jamal, Lisa Branzell
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 13994
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The natural smallRNA spot42 taken from the E-coli MG1655. SmallRNA is belived to interact in many metabolic pathways. The spot42 scaffold interacts with HFQ-proteins. It is in many cases shown to bind into the rbs area of the mRNA and thereby blocking translation initiation. ( V.Sharma, A.Yamamura Y.Yokobayashi, 2011 ) We wanted to use this sRNA for gene downregulation on translational level, in this case block the ribosome from binding to the rbs area. You can use this natural scaffold to make your own sRNA, inhibiting translation on a gene of your choice. The sRNA is coupled to a strong promotor, J23101
mutable notes: It can be a bit tricky to do mutagenesis on it, like we did. We wanted to add a a random antisense area with primers. You cannot have any scars between the promotor and sRNA so we added our promotor with mutagenesis.
mutable provenance: It was biobricked from E-coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Spot42_Antisense
sequence annotation: J23101
sequence annotation: Spot42_Scaffold
sequence: https://synbiohub.org/public/igem/BBa_K864440_sequence/1

Spot42_Antisense
type: SequenceAnnotation
persistent identity: annotation2198851
display id: annotation2198851
version: 1
top level: BBa_K864440
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864440/annotation2198851/range2198851/1

https://synbiohub.org/public/igem/BBa_K864440/annotation2198851/range2198851/1
type: Range
persistent identity: range2198851
display id: range2198851
version: 1
direction: direction/forward
top level: BBa_K864440
start: 36
end: 91
orientation: inline

J23101
type: SequenceAnnotation
persistent identity: annotation2198848
display id: annotation2198848
version: 1
top level: BBa_K864440
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864440/annotation2198848/range2198848/1

https://synbiohub.org/public/igem/BBa_K864440/annotation2198848/range2198848/1
type: Range
persistent identity: range2198848
display id: range2198848
version: 1
direction: direction/forward
top level: BBa_K864440
start: 1
end: 35
orientation: inline

Spot42_Scaffold
type: SequenceAnnotation
persistent identity: annotation2198854
display id: annotation2198854
version: 1
top level: BBa_K864440
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864440/annotation2198854/range2198854/1

https://synbiohub.org/public/igem/BBa_K864440/annotation2198854/range2198854/1
type: Range
persistent identity: range2198854
display id: range2198854
version: 1
direction: direction/forward
top level: BBa_K864440
start: 92
end: 144
orientation: inline

BBa_K737017
type: ComponentDefinition
persistent identity: BBa K737017
display id: BBa_K737017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This part conteins gvpC-20psi protein
creator: Jiaheng Li
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part conteins gvpA protein's coding sequence. This gene from Planktothrix rubescens can be succesfully expressed in E. coli. The gvpA's expression have been examined by another part " J23106+B0034+gvpA+B0015". (Since it was ligated on cloning vector pSB4A5, we didn't register this part. The gvpA protein conteins a typical domain of the superfamily 'Gas vesicle'(NCBI CDD cl02594). This gene is quiet conserved in most gvp's polycistron. According to Anthony E. Walsby's research, gas vesicles have a similar morphology. GvpA protein is a kind of small hydrophobic protein which forms the ribs of the main structure. 'All cyanobacterial gas vesicles so far analyzed contain a protein (GvpA) of about 7.4 kDa that forms the main mass of the structure and must be responsible for many of its properties.' (Gas Vesicles. Microbiological Reviews. A. E. Walsby)
mutable notes: The gvpA protein conteins a typical domain of the superfamily 'Gas vesicle'(NCBI CDD cl02594). This gene is quiet conserved in most gvp's polycistron. According to Anthony E. Walsby's research, gas vesicles have a similar morphology. GvpA protein is a kind of small hydrophobic protein which forms the ribs of the main structure. 'All cyanobacterial gas vesicles so far analyzed contain a protein (GvpA) of about 7.4 kDa that forms the main mass of the structure and must be responsible for many of its properties.' (Gas Vesicles. Microbiological Reviews. A. E. Walsby)
mutable provenance: Yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K737017_sequence/1

BBa_K660610
type: ComponentDefinition
persistent identity: BBa K660610
display id: BBa_K660610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + RcsB
creator: Glasgow Uni 2011
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:13:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _837_
m user id: 0
owner id: 9433
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It's a transcription factor for Colanic acid with a strong RBS
mutable notes: It's an intermediate
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_K200000
sequence annotation: BBa_B0030
sequence annotation: BBa_K200000
sequence: https://synbiohub.org/public/igem/BBa_K660610_sequence/1

BBa_B0030
type: Component
persistent identity: component2137345
display id: component2137345
version: 1
top level: BBa_K660610
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K200000
type: Component
persistent identity: component2137350
display id: component2137350
version: 1
top level: BBa_K660610
access: public
definition: https://synbiohub.org/public/igem/BBa_K200000/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2137345
display id: annotation2137345
version: 1
top level: BBa_K660610
location: https://synbiohub.org/public/igem/BBa_K660610/annotation2137345/range2137345/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K660610/annotation2137345/range2137345/1
type: Range
persistent identity: range2137345
display id: range2137345
version: 1
direction: direction/forward
top level: BBa_K660610
start: 1
end: 15
orientation: inline

BBa_K200000
type: SequenceAnnotation
persistent identity: annotation2137350
display id: annotation2137350
version: 1
top level: BBa_K660610
location: https://synbiohub.org/public/igem/BBa_K660610/annotation2137350/range2137350/1
component: BBa_K200000

https://synbiohub.org/public/igem/BBa_K660610/annotation2137350/range2137350/1
type: Range
persistent identity: range2137350
display id: range2137350
version: 1
direction: direction/reverse
top level: BBa_K660610
start: 22
end: 675
orientation: inline

BBa_K575021
type: ComponentDefinition
persistent identity: BBa K575021
display id: BBa_K575021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Genomic LasR/PAI1 Inducible Promoter (Short)
creator: Michael Vincent Sherer
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8753
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the PAI-1/LasR complex, part of the Pseudomonas quorum sensing hierarchy.
mutable notes: The part is unusually long for a promoter because of the primers we used to extract it (it is called short because it is shorter than companion part BBa_K575020. We do not know exactly where the regulatory region is.
mutable provenance: It is the upstream regulatory region of [gene].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K575021_sequence/1

BBa_K554004
type: ComponentDefinition
persistent identity: BBa K554004
display id: BBa_K554004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IL10
creator: UNICAMP EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: IL10 is ...
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IL10
sequence annotation: stop
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K554004_sequence/1

IL10
type: SequenceAnnotation
persistent identity: annotation2136877
display id: annotation2136877
version: 1
top level: BBa_K554004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K554004/annotation2136877/range2136877/1

https://synbiohub.org/public/igem/BBa_K554004/annotation2136877/range2136877/1
type: Range
persistent identity: range2136877
display id: range2136877
version: 1
direction: direction/forward
top level: BBa_K554004
start: 16
end: 497
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2136876
display id: annotation2136876
version: 1
top level: BBa_K554004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K554004/annotation2136876/range2136876/1

https://synbiohub.org/public/igem/BBa_K554004/annotation2136876/range2136876/1
type: Range
persistent identity: range2136876
display id: range2136876
version: 1
direction: direction/forward
top level: BBa_K554004
start: 498
end: 504
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136875
display id: annotation2136875
version: 1
top level: BBa_K554004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K554004/annotation2136875/range2136875/1

https://synbiohub.org/public/igem/BBa_K554004/annotation2136875/range2136875/1
type: Range
persistent identity: range2136875
display id: range2136875
version: 1
direction: direction/forward
top level: BBa_K554004
start: 1
end: 15
orientation: inline

Tsi2
type: ComponentDefinition
persistent identity: BBa K314201
display id: BBa_K314201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Antitoxin Tsi2
creator: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Protein originating from pseudomonas aeruginosa that confers immunity to the toxic protein Tse2
mutable provenance: pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tsi2
sequence: https://synbiohub.org/public/igem/BBa_K314201_sequence/1

tsi2
type: SequenceAnnotation
persistent identity: annotation2089457
display id: annotation2089457
version: 1
top level: Tsi2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K314201/annotation2089457/range2089457/1

https://synbiohub.org/public/igem/BBa_K314201/annotation2089457/range2089457/1
type: Range
persistent identity: range2089457
display id: range2089457
version: 1
direction: direction/forward
top level: Tsi2
start: 1
end: 234
orientation: inline

Aeq
type: ComponentDefinition
persistent identity: BBa K548000
display id: BBa_K548000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Humanized Aequorin
creator: Peter Qiao
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _716_
m user id: 0
owner id: 8882
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a human cell line optimized version of Aequorin, a bioluminescent protein found in various jellyfish and other marine organisms. The protein requires Ca2+ for catalytic activity. And oxidizes its substrate, Coelenterazine to emit blue light (λmax=469nm). Aequorin is NOT a fluorescent protein, it does not require an excitation light source to emit light. Transfection of the gene into 293T cells using lipofectamine has been confirmed. The part contains EcoRI and PstI restrictions sites, and is ligated to the iGEM pSB1C3 backbone. This backbone provides chloramphenicol resistance.
mutable notes: Original sequence had to be modified for increased expression in human cell lines.
mutable provenance: Synthesized from GeneArt, original sequence from NCBI, optimized using GeneArt codon optimization software.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K548000_sequence/1

BBa_K608011
type: ComponentDefinition
persistent identity: BBa K608011
display id: BBa_K608011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium promoter with medium RBS and GFP
creator: Sandra Wassner
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 10299
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium promotor from the constitutive promotor family combined with medium RBS and GFP. To quantify the gene expression, GFP was tagged to the promotor RBS domain.
mutable notes: We made this composite to test the effiency of the gene expression.
mutable provenance: Composite from parts registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23110
component: BBa_E0040
component: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_J23110
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K608011_sequence/1

BBa_J23110
type: Component
persistent identity: component2128034
display id: component2128034
version: 1
top level: BBa_K608011
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_E0040
type: Component
persistent identity: component2128039
display id: component2128039
version: 1
top level: BBa_K608011
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0032
type: Component
persistent identity: component2128036
display id: component2128036
version: 1
top level: BBa_K608011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2128039
display id: annotation2128039
version: 1
top level: BBa_K608011
location: https://synbiohub.org/public/igem/BBa_K608011/annotation2128039/range2128039/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K608011/annotation2128039/range2128039/1
type: Range
persistent identity: range2128039
display id: range2128039
version: 1
direction: direction/reverse
top level: BBa_K608011
start: 63
end: 782
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2128034
display id: annotation2128034
version: 1
top level: BBa_K608011
location: https://synbiohub.org/public/igem/BBa_K608011/annotation2128034/range2128034/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K608011/annotation2128034/range2128034/1
type: Range
persistent identity: range2128034
display id: range2128034
version: 1
direction: direction/reverse
top level: BBa_K608011
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128036
display id: annotation2128036
version: 1
top level: BBa_K608011
location: https://synbiohub.org/public/igem/BBa_K608011/annotation2128036/range2128036/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K608011/annotation2128036/range2128036/1
type: Range
persistent identity: range2128036
display id: range2128036
version: 1
direction: direction/forward
top level: BBa_K608011
start: 44
end: 56
orientation: inline

BBa_K577894
type: ComponentDefinition
persistent identity: BBa K577894
display id: BBa_K577894
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressed YFP
creator: Kyle Jones
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _748_
m user id: 0
owner id: 8430
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A composite part containing the TetR gene, C0040, turned on by a constitutive promoter, I14033. The TetR represses the pTet promoter in front of the YFP gene. By addition of tetracycline or aTc, this repression is inhibited and the cells should fluoresce yellow.
mutable notes: The whole part is in the backbone of I14033. Both P0440, the TetR composite part, and E0430, the YFP composite part, were used and inserted into their respective promoters. The pTet+YFP part was then combined as a suffix insertion to the I14033+TetR backbone.
mutable provenance: Spring 2011 iGEM Kit Plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_C0040
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_E0030
component: BBa_I14033
sequence annotation: BBa_I14033
sequence annotation: BBa_R0040
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K577894_sequence/1

BBa_C0040
type: Component
persistent identity: component2289606
display id: component2289606
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0034
type: Component
persistent identity: component2289619
display id: component2289619
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2289609
display id: component2289609
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2289621
display id: component2289621
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component2289607
display id: component2289607
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2289602
display id: component2289602
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2289623
display id: component2289623
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component2289613
display id: component2289613
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0030
type: Component
persistent identity: component2289620
display id: component2289620
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_I14033
type: Component
persistent identity: component2289600
display id: component2289600
version: 1
top level: BBa_K577894
access: public
definition: https://synbiohub.org/public/igem/BBa_I14033/1

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2289600
display id: annotation2289600
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289600/range2289600/1
component: BBa_I14033

https://synbiohub.org/public/igem/BBa_K577894/annotation2289600/range2289600/1
type: Range
persistent identity: range2289600
display id: range2289600
version: 1
direction: direction/reverse
top level: BBa_K577894
start: 1
end: 38
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2289613
display id: annotation2289613
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289613/range2289613/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K577894/annotation2289613/range2289613/1
type: Range
persistent identity: range2289613
display id: range2289613
version: 1
direction: direction/forward
top level: BBa_K577894
start: 895
end: 948
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2289620
display id: annotation2289620
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289620/range2289620/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K577894/annotation2289620/range2289620/1
type: Range
persistent identity: range2289620
display id: range2289620
version: 1
direction: direction/reverse
top level: BBa_K577894
start: 975
end: 1697
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2289619
display id: annotation2289619
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289619/range2289619/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K577894/annotation2289619/range2289619/1
type: Range
persistent identity: range2289619
display id: range2289619
version: 1
direction: direction/reverse
top level: BBa_K577894
start: 957
end: 968
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2289621
display id: annotation2289621
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289621/range2289621/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K577894/annotation2289621/range2289621/1
type: Range
persistent identity: range2289621
display id: range2289621
version: 1
direction: direction/forward
top level: BBa_K577894
start: 1706
end: 1785
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2289607
display id: annotation2289607
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289607/range2289607/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K577894/annotation2289607/range2289607/1
type: Range
persistent identity: range2289607
display id: range2289607
version: 1
direction: direction/forward
top level: BBa_K577894
start: 758
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2289602
display id: annotation2289602
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289602/range2289602/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K577894/annotation2289602/range2289602/1
type: Range
persistent identity: range2289602
display id: range2289602
version: 1
direction: direction/reverse
top level: BBa_K577894
start: 47
end: 58
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2289609
display id: annotation2289609
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289609/range2289609/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K577894/annotation2289609/range2289609/1
type: Range
persistent identity: range2289609
display id: range2289609
version: 1
direction: direction/forward
top level: BBa_K577894
start: 846
end: 886
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2289606
display id: annotation2289606
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289606/range2289606/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K577894/annotation2289606/range2289606/1
type: Range
persistent identity: range2289606
display id: range2289606
version: 1
direction: direction/reverse
top level: BBa_K577894
start: 65
end: 749
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2289623
display id: annotation2289623
version: 1
top level: BBa_K577894
location: https://synbiohub.org/public/igem/BBa_K577894/annotation2289623/range2289623/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K577894/annotation2289623/range2289623/1
type: Range
persistent identity: range2289623
display id: range2289623
version: 1
direction: direction/forward
top level: BBa_K577894
start: 1794
end: 1834
orientation: inline

BBa_K541506
type: ComponentDefinition
persistent identity: BBa K541506
display id: BBa_K541506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reflectin1A from Cephalopod
creator: Fazilet Guler
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _708_
m user id: 0
owner id: 8463
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli.
mutable notes: Optimized for both B.subtilis and E.coli
mutable provenance: Cephalopod
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Reflectin
sequence: https://synbiohub.org/public/igem/BBa_K541506_sequence/1

Reflectin
type: SequenceAnnotation
persistent identity: annotation2123461
display id: annotation2123461
version: 1
top level: BBa_K541506
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K541506/annotation2123461/range2123461/1

https://synbiohub.org/public/igem/BBa_K541506/annotation2123461/range2123461/1
type: Range
persistent identity: range2123461
display id: range2123461
version: 1
direction: direction/forward
top level: BBa_K541506
start: 1
end: 855
orientation: inline

hycAp
type: ComponentDefinition
persistent identity: BBa K778000
display id: BBa_K778000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hycAp: Promoter of hyc operon activated by FhlA
creator: Yuka Yamazaki
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: hycAp is promoter activated by FhlA (transcriptional activater)
mutable notes: mutation?
mutable provenance: Escherichia coli (JM109)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FhlA-binding site-2
sequence annotation: Transcription Start Site
sequence annotation: FhlA-binding site-1
sequence: https://synbiohub.org/public/igem/BBa_K778000_sequence/1

FhlA-binding site-2
type: SequenceAnnotation
persistent identity: annotation2202206
display id: annotation2202206
version: 1
top level: hycAp
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778000/annotation2202206/range2202206/1

https://synbiohub.org/public/igem/BBa_K778000/annotation2202206/range2202206/1
type: Range
persistent identity: range2202206
display id: range2202206
version: 1
direction: direction/forward
top level: hycAp
start: 42
end: 76
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation2192657
display id: annotation2192657
version: 1
top level: hycAp
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K778000/annotation2192657/range2192657/1

https://synbiohub.org/public/igem/BBa_K778000/annotation2192657/range2192657/1
type: Range
persistent identity: range2192657
display id: range2192657
version: 1
direction: direction/forward
top level: hycAp
start: 143
end: 143
orientation: inline

FhlA-binding site-1
type: SequenceAnnotation
persistent identity: annotation2202204
display id: annotation2202204
version: 1
top level: hycAp
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778000/annotation2202204/range2202204/1

https://synbiohub.org/public/igem/BBa_K778000/annotation2202204/range2202204/1
type: Range
persistent identity: range2202204
display id: range2202204
version: 1
direction: direction/forward
top level: hycAp
start: 29
end: 61
orientation: inline

BBa_K611014
type: ComponentDefinition
persistent identity: BBa K611014
display id: BBa_K611014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI Repressible Promoter Characterization Construct
creator: Nicholas Csicsery
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used to characterize mutants of BBa_R0051 (c1 repressible promoter). By adding a mutant of BBa_R0051 before this part, one can measure several of its properties through the expression of GFP. Certain characteristics of the mutant, such as the binding of the c1 repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.
mutable notes: There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.
mutable provenance: Built from existing parts in the registry though BioBrick assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I13453
component: BBa_E0040
component: BBa_C0051
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_C0051
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K611014_sequence/1

BBa_B0034
type: Component
persistent identity: component2268760
display id: component2268760
version: 1
top level: BBa_K611014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13453
type: Component
persistent identity: component2268770
display id: component2268770
version: 1
top level: BBa_K611014
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_E0040
type: Component
persistent identity: component2268762
display id: component2268762
version: 1
top level: BBa_K611014
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_C0051
type: Component
persistent identity: component2268776
display id: component2268776
version: 1
top level: BBa_K611014
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0015
type: Component
persistent identity: component2268769
display id: component2268769
version: 1
top level: BBa_K611014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2268772
display id: component2268772
version: 1
top level: BBa_K611014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2268769
display id: annotation2268769
version: 1
top level: BBa_K611014
location: https://synbiohub.org/public/igem/BBa_K611014/annotation2268769/range2268769/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K611014/annotation2268769/range2268769/1
type: Range
persistent identity: range2268769
display id: range2268769
version: 1
direction: direction/reverse
top level: BBa_K611014
start: 747
end: 875
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2268776
display id: annotation2268776
version: 1
top level: BBa_K611014
location: https://synbiohub.org/public/igem/BBa_K611014/annotation2268776/range2268776/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K611014/annotation2268776/range2268776/1
type: Range
persistent identity: range2268776
display id: range2268776
version: 1
direction: direction/reverse
top level: BBa_K611014
start: 1040
end: 1814
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2268770
display id: annotation2268770
version: 1
top level: BBa_K611014
location: https://synbiohub.org/public/igem/BBa_K611014/annotation2268770/range2268770/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K611014/annotation2268770/range2268770/1
type: Range
persistent identity: range2268770
display id: range2268770
version: 1
direction: direction/reverse
top level: BBa_K611014
start: 884
end: 1013
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2268772
display id: annotation2268772
version: 1
top level: BBa_K611014
location: https://synbiohub.org/public/igem/BBa_K611014/annotation2268772/range2268772/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611014/annotation2268772/range2268772/1
type: Range
persistent identity: range2268772
display id: range2268772
version: 1
direction: direction/reverse
top level: BBa_K611014
start: 1022
end: 1033
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2268760
display id: annotation2268760
version: 1
top level: BBa_K611014
location: https://synbiohub.org/public/igem/BBa_K611014/annotation2268760/range2268760/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611014/annotation2268760/range2268760/1
type: Range
persistent identity: range2268760
display id: range2268760
version: 1
direction: direction/reverse
top level: BBa_K611014
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2268762
display id: annotation2268762
version: 1
top level: BBa_K611014
location: https://synbiohub.org/public/igem/BBa_K611014/annotation2268762/range2268762/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K611014/annotation2268762/range2268762/1
type: Range
persistent identity: range2268762
display id: range2268762
version: 1
direction: direction/reverse
top level: BBa_K611014
start: 19
end: 738
orientation: inline

pHS-tRNAAm
type: ComponentDefinition
persistent identity: BBa K606041
display id: BBa_K606041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pHyperspank Tyrosine amber supressor tRNA double terminator
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Tyrosine amber supressor tRNA expression system induced by IPTG in presence of LacI
mutable notes: biobrick assembly
mutable provenance: B. subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K606040
component: BBa_K606034
sequence annotation: BBa_K606034
sequence annotation: BBa_K606040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K606041_sequence/1

BBa_B0015
type: Component
persistent identity: component2160456
display id: component2160456
version: 1
top level: pHS-tRNAAm
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K606040
type: Component
persistent identity: component2160448
display id: component2160448
version: 1
top level: pHS-tRNAAm
access: public
definition: pHS

BBa_K606034
type: Component
persistent identity: component2160449
display id: component2160449
version: 1
top level: pHS-tRNAAm
access: public
definition: Y taRNA

BBa_K606034
type: SequenceAnnotation
persistent identity: annotation2160449
display id: annotation2160449
version: 1
top level: pHS-tRNAAm
location: https://synbiohub.org/public/igem/BBa_K606041/annotation2160449/range2160449/1
component: BBa_K606034

https://synbiohub.org/public/igem/BBa_K606041/annotation2160449/range2160449/1
type: Range
persistent identity: range2160449
display id: range2160449
version: 1
direction: direction/reverse
top level: pHS-tRNAAm
start: 101
end: 185
orientation: inline

BBa_K606040
type: SequenceAnnotation
persistent identity: annotation2160448
display id: annotation2160448
version: 1
top level: pHS-tRNAAm
location: https://synbiohub.org/public/igem/BBa_K606041/annotation2160448/range2160448/1
component: BBa_K606040

https://synbiohub.org/public/igem/BBa_K606041/annotation2160448/range2160448/1
type: Range
persistent identity: range2160448
display id: range2160448
version: 1
direction: direction/reverse
top level: pHS-tRNAAm
start: 1
end: 92
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2160456
display id: annotation2160456
version: 1
top level: pHS-tRNAAm
location: https://synbiohub.org/public/igem/BBa_K606041/annotation2160456/range2160456/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K606041/annotation2160456/range2160456/1
type: Range
persistent identity: range2160456
display id: range2160456
version: 1
direction: direction/reverse
top level: pHS-tRNAAm
start: 194
end: 322
orientation: inline

BBa_K592026
type: ComponentDefinition
persistent identity: BBa K592026
display id: BBa_K592026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101-B0032-BFP
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reference promoter BBa_J23101 for testing the promoter strength coupled with BFP (( BBa_K592100 )). Once this is cloned into pSB3K3 backbone one can measure the BFP output and use it as reference value to measure the promoter strength of other promoters.
mutable notes: The BFP part ( BBa_K592100 ) was codon-optimized for expression in E.coli by DNA2.0
mutable provenance: The BFP part is BBa_K592100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0032
component: BBa_K592100
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_K592100
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K592026_sequence/1

BBa_B0032
type: Component
persistent identity: component2136727
display id: component2136727
version: 1
top level: BBa_K592026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K592100
type: Component
persistent identity: component2136730
display id: component2136730
version: 1
top level: BBa_K592026
access: public
definition: BFP

BBa_J23101
type: Component
persistent identity: component2136725
display id: component2136725
version: 1
top level: BBa_K592026
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2136725
display id: annotation2136725
version: 1
top level: BBa_K592026
location: https://synbiohub.org/public/igem/BBa_K592026/annotation2136725/range2136725/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K592026/annotation2136725/range2136725/1
type: Range
persistent identity: range2136725
display id: range2136725
version: 1
direction: direction/reverse
top level: BBa_K592026
start: 1
end: 35
orientation: inline

BBa_K592100
type: SequenceAnnotation
persistent identity: annotation2136730
display id: annotation2136730
version: 1
top level: BBa_K592026
location: https://synbiohub.org/public/igem/BBa_K592026/annotation2136730/range2136730/1
component: BBa_K592100

https://synbiohub.org/public/igem/BBa_K592026/annotation2136730/range2136730/1
type: Range
persistent identity: range2136730
display id: range2136730
version: 1
direction: direction/reverse
top level: BBa_K592026
start: 63
end: 767
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2136727
display id: annotation2136727
version: 1
top level: BBa_K592026
location: https://synbiohub.org/public/igem/BBa_K592026/annotation2136727/range2136727/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K592026/annotation2136727/range2136727/1
type: Range
persistent identity: range2136727
display id: range2136727
version: 1
direction: direction/forward
top level: BBa_K592026
start: 44
end: 56
orientation: inline

AAR1C3
type: ComponentDefinition
persistent identity: BBa K590027
display id: BBa_K590027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AAR-PSB1C3-High consititutive
creator: Lei Zheng
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Acyl-ACP Reductase in PSB1C3 high consititutive vector
mutable notes: None
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K314100
component: BBa_K590032
sequence annotation: BBa_K590032
sequence annotation: BBa_K314100
sequence: https://synbiohub.org/public/igem/BBa_K590027_sequence/1

BBa_K314100
type: Component
persistent identity: component2129121
display id: component2129121
version: 1
top level: AAR1C3
access: public
definition: BBa_K314100

BBa_K590032
type: Component
persistent identity: component2129122
display id: component2129122
version: 1
top level: AAR1C3
access: public
definition: https://synbiohub.org/public/igem/BBa_K590032/1

BBa_K590032
type: SequenceAnnotation
persistent identity: annotation2129122
display id: annotation2129122
version: 1
top level: AAR1C3
location: https://synbiohub.org/public/igem/BBa_K590027/annotation2129122/range2129122/1
component: BBa_K590032

https://synbiohub.org/public/igem/BBa_K590027/annotation2129122/range2129122/1
type: Range
persistent identity: range2129122
display id: range2129122
version: 1
direction: direction/reverse
top level: AAR1C3
start: 525
end: 1610
orientation: inline

BBa_K314100
type: SequenceAnnotation
persistent identity: annotation2129121
display id: annotation2129121
version: 1
top level: AAR1C3
location: https://synbiohub.org/public/igem/BBa_K590027/annotation2129121/range2129121/1
component: BBa_K314100

https://synbiohub.org/public/igem/BBa_K590027/annotation2129121/range2129121/1
type: Range
persistent identity: range2129121
display id: range2129121
version: 1
direction: direction/reverse
top level: AAR1C3
start: 1
end: 518
orientation: inline

BBa_K576006
type: ComponentDefinition
persistent identity: BBa K576006
display id: BBa_K576006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP2-Transcription Terminator
creator: Joon Hyuk Hong
created: 2011-06-19T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _747_
m user id: 0
owner id: 10155
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The RFC 53 TailPart with transcription terminator.
mutable notes: The format is RFC53 D-TailPart, i.e. ATG 5' overhang.
mutable provenance: GFP2 by Davidson-Missouri 2007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K576002
sequence annotation: BBa_B0015
sequence annotation: BBa_K576002
sequence: https://synbiohub.org/public/igem/BBa_K576006_sequence/1

BBa_B0015
type: Component
persistent identity: component2121653
display id: component2121653
version: 1
top level: BBa_K576006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K576002
type: Component
persistent identity: component2121646
display id: component2121646
version: 1
top level: BBa_K576006
access: public
definition: https://synbiohub.org/public/igem/BBa_K576002/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2121653
display id: annotation2121653
version: 1
top level: BBa_K576006
location: https://synbiohub.org/public/igem/BBa_K576006/annotation2121653/range2121653/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K576006/annotation2121653/range2121653/1
type: Range
persistent identity: range2121653
display id: range2121653
version: 1
direction: direction/reverse
top level: BBa_K576006
start: 271
end: 399
orientation: inline

BBa_K576002
type: SequenceAnnotation
persistent identity: annotation2121646
display id: annotation2121646
version: 1
top level: BBa_K576006
location: https://synbiohub.org/public/igem/BBa_K576006/annotation2121646/range2121646/1
component: BBa_K576002

https://synbiohub.org/public/igem/BBa_K576006/annotation2121646/range2121646/1
type: Range
persistent identity: range2121646
display id: range2121646
version: 1
direction: direction/reverse
top level: BBa_K576006
start: 1
end: 262
orientation: inline

BBa_K541503
type: ComponentDefinition
persistent identity: BBa K541503
display id: BBa_K541503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Strong promoter, Strong RBS and TAT signal peptide
creator: Mustafa Elitok
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _708_
m user id: 0
owner id: 9573
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Twin arginine signal peptide is an efficient protein export signal sequence of E. coli. Because of its constitutive character, its promoter works permanently.
mutable notes: Optimized for E.coli
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: conserved
sequence annotation: TAT signaling peptide
sequence: https://synbiohub.org/public/igem/BBa_K541503_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2123465
display id: annotation2123465
version: 1
top level: BBa_K541503
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K541503/annotation2123465/range2123465/1

https://synbiohub.org/public/igem/BBa_K541503/annotation2123465/range2123465/1
type: Range
persistent identity: range2123465
display id: range2123465
version: 1
direction: direction/reverse
top level: BBa_K541503
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2123463
display id: annotation2123463
version: 1
top level: BBa_K541503
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K541503/annotation2123463/range2123463/1

https://synbiohub.org/public/igem/BBa_K541503/annotation2123463/range2123463/1
type: Range
persistent identity: range2123463
display id: range2123463
version: 1
direction: direction/reverse
top level: BBa_K541503
start: 1
end: 35
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2123464
display id: annotation2123464
version: 1
top level: BBa_K541503
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K541503/annotation2123464/range2123464/1

https://synbiohub.org/public/igem/BBa_K541503/annotation2123464/range2123464/1
type: Range
persistent identity: range2123464
display id: range2123464
version: 1
direction: direction/forward
top level: BBa_K541503
start: 48
end: 51
orientation: inline

TAT signaling peptide
type: SequenceAnnotation
persistent identity: annotation2123466
display id: annotation2123466
version: 1
top level: BBa_K541503
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K541503/annotation2123466/range2123466/1

https://synbiohub.org/public/igem/BBa_K541503/annotation2123466/range2123466/1
type: Range
persistent identity: range2123466
display id: range2123466
version: 1
direction: direction/forward
top level: BBa_K541503
start: 62
end: 151
orientation: inline

BBa_K606017
type: ComponentDefinition
persistent identity: BBa K606017
display id: BBa_K606017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 polymerase expression casette for Bacillus Subtilis
creator: Cyrille Pauthenier
created: 2011-07-06T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
group u list: _778_
m user id: 0
owner id: 8998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fused with the RBS SpoVG, this biobrick is the expression cassette for B. Subtilis of the T7 polymerase.
mutable notes: none
mutable provenance: Biobricks only (RBS sythetyzed de novo)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K143021
component: BBa_K145001
sequence annotation: BBa_K143021
sequence annotation: BBa_K145001
sequence: https://synbiohub.org/public/igem/BBa_K606017_sequence/1

BBa_K143021
type: Component
persistent identity: component2123197
display id: component2123197
version: 1
top level: BBa_K606017
access: public
definition: https://synbiohub.org/public/igem/BBa_K143021/1

BBa_K145001
type: Component
persistent identity: component2123202
display id: component2123202
version: 1
top level: BBa_K606017
access: public
definition: https://synbiohub.org/public/igem/BBa_K145001/1

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2123197
display id: annotation2123197
version: 1
top level: BBa_K606017
location: https://synbiohub.org/public/igem/BBa_K606017/annotation2123197/range2123197/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K606017/annotation2123197/range2123197/1
type: Range
persistent identity: range2123197
display id: range2123197
version: 1
direction: direction/reverse
top level: BBa_K606017
start: 1
end: 12
orientation: inline

BBa_K145001
type: SequenceAnnotation
persistent identity: annotation2123202
display id: annotation2123202
version: 1
top level: BBa_K606017
location: https://synbiohub.org/public/igem/BBa_K606017/annotation2123202/range2123202/1
component: BBa_K145001

https://synbiohub.org/public/igem/BBa_K606017/annotation2123202/range2123202/1
type: Range
persistent identity: range2123202
display id: range2123202
version: 1
direction: direction/reverse
top level: BBa_K606017
start: 19
end: 2673
orientation: inline

BBa_K813002
type: ComponentDefinition
persistent identity: BBa K813002
display id: BBa_K813002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HAP4 - Yeast Genomic IRES
creator: Morgan Baltz, Katie Lutes, Akshitha Yarrabothula, Michael Wierzbicki
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1070_
m user id: 0
owner id: 11253
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: IRES elements initiate 5'- independent translation.
mutable notes: NONE
mutable provenance: saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K813002_sequence/1

BBa_K823006
type: ComponentDefinition
persistent identity: BBa K823006
display id: BBa_K823006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23102
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:45:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23102 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23102
sequence annotation: -10 box
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823006_sequence/1

J23102
type: SequenceAnnotation
persistent identity: annotation2182512
display id: annotation2182512
version: 1
top level: BBa_K823006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1
type: Range
persistent identity: range2182512
display id: range2182512
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182514
display id: annotation2182514
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1
type: Range
persistent identity: range2182514
display id: range2182514
version: 1
direction: direction/forward
top level: BBa_K823006
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182513
display id: annotation2182513
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1
type: Range
persistent identity: range2182513
display id: range2182513
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 6
orientation: inline

BBa_K540001
type: ComponentDefinition
persistent identity: BBa K540001
display id: BBa_K540001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rcn, cobalt-sensitive promoter
creator: Mathilde Dumond
created: 2011-09-14T11:00:00Z
modified: 2015-07-14T12:34:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _706_
m user id: 4206
owner id: 8419
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Rcn is a promoter that is activated by cobalt. A repressor is fixed to the promoter by default, and cobalt binds to this repressor, which activates the gene under control of this promoter. Rcn is also activated by nickel, but we have not characterized this aspect.
mutable notes: An internal iGEM restriction site had to be mutated by QuickChange mutagenesis.
mutable provenance: PCR from E.coli MC4100 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RcnR
sequence: https://synbiohub.org/public/igem/BBa_K540001_sequence/1

RcnR
type: SequenceAnnotation
persistent identity: annotation2129035
display id: annotation2129035
version: 1
top level: BBa_K540001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K540001/annotation2129035/range2129035/1

https://synbiohub.org/public/igem/BBa_K540001/annotation2129035/range2129035/1
type: Range
persistent identity: range2129035
display id: range2129035
version: 1
direction: direction/reverse
top level: BBa_K540001
start: 1
end: 279
orientation: inline

BBa_S04617
type: ComponentDefinition
persistent identity: BBa S04617
display id: BBa_S04617
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K592006:Q04510
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:14:42Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q04510
component: BBa_K592006
sequence annotation: BBa_Q04510
sequence annotation: BBa_K592006
sequence: https://synbiohub.org/public/igem/BBa_S04617_sequence/1

BBa_Q04510
type: Component
persistent identity: component2137040
display id: component2137040
version: 1
top level: BBa_S04617
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04510/1

BBa_K592006
type: Component
persistent identity: component2137022
display id: component2137022
version: 1
top level: BBa_S04617
access: public
definition: BBa_K592006

BBa_Q04510
type: SequenceAnnotation
persistent identity: annotation2137040
display id: annotation2137040
version: 1
top level: BBa_S04617
location: https://synbiohub.org/public/igem/BBa_S04617/annotation2137040/range2137040/1
component: BBa_Q04510

https://synbiohub.org/public/igem/BBa_S04617/annotation2137040/range2137040/1
type: Range
persistent identity: range2137040
display id: range2137040
version: 1
direction: direction/reverse
top level: BBa_S04617
start: 259
end: 1245
orientation: inline

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2137022
display id: annotation2137022
version: 1
top level: BBa_S04617
location: https://synbiohub.org/public/igem/BBa_S04617/annotation2137022/range2137022/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_S04617/annotation2137022/range2137022/1
type: Range
persistent identity: range2137022
display id: range2137022
version: 1
direction: direction/reverse
top level: BBa_S04617
start: 1
end: 250
orientation: inline

BBa_K569017
type: ComponentDefinition
persistent identity: BBa K569017
display id: BBa_K569017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY
creator: David Golynskiy and Tyler Guinn
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _737_
m user id: 0
owner id: 9213
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ?
mutable notes: ?
mutable provenance: ?
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K569017_sequence/1

BBa_K598008
type: ComponentDefinition
persistent identity: BBa K598008
display id: BBa_K598008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015
creator: Yangyang ZHAO, Yan GONG
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8817
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 1.20+RBS+E0040+B0015
mutable notes: This part contains a native ribosomal binding site.
mutable provenance: Cloning from the parts BBa_K598012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eGFP
sequence annotation: BBa_B0015
sequence annotation: TPP Ribozyme 1.20
sequence annotation: BBa_E0040
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K598008_sequence/1

eGFP
type: SequenceAnnotation
persistent identity: annotation2152740
display id: annotation2152740
version: 1
top level: BBa_K598008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598008/annotation2152740/range2152740/1

https://synbiohub.org/public/igem/BBa_K598008/annotation2152740/range2152740/1
type: Range
persistent identity: range2152740
display id: range2152740
version: 1
direction: direction/forward
top level: BBa_K598008
start: 151
end: 870
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153016
display id: annotation2153016
version: 1
top level: BBa_K598008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598008/annotation2153016/range2153016/1

https://synbiohub.org/public/igem/BBa_K598008/annotation2153016/range2153016/1
type: Range
persistent identity: range2153016
display id: range2153016
version: 1
direction: direction/forward
top level: BBa_K598008
start: 879
end: 1007
orientation: inline

TPP Ribozyme 1.20
type: SequenceAnnotation
persistent identity: annotation2152738
display id: annotation2152738
version: 1
top level: BBa_K598008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598008/annotation2152738/range2152738/1

https://synbiohub.org/public/igem/BBa_K598008/annotation2152738/range2152738/1
type: Range
persistent identity: range2152738
display id: range2152738
version: 1
direction: direction/forward
top level: BBa_K598008
start: 1
end: 150
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2153015
display id: annotation2153015
version: 1
top level: BBa_K598008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598008/annotation2153015/range2153015/1

https://synbiohub.org/public/igem/BBa_K598008/annotation2153015/range2153015/1
type: Range
persistent identity: range2153015
display id: range2153015
version: 1
direction: direction/forward
top level: BBa_K598008
start: 151
end: 870
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2152739
display id: annotation2152739
version: 1
top level: BBa_K598008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598008/annotation2152739/range2152739/1

https://synbiohub.org/public/igem/BBa_K598008/annotation2152739/range2152739/1
type: Range
persistent identity: range2152739
display id: range2152739
version: 1
direction: direction/forward
top level: BBa_K598008
start: 137
end: 145
orientation: inline

BBa_K581006
type: ComponentDefinition
persistent identity: BBa K581006
display id: BBa_K581006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc+ptsG(wt)-GFP (ptsG(wt) 5' UTR fused with gfp)
creator: Qin Xiao
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _752_
m user id: 0
owner id: 8589
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the conjugate part of the small RNA regulator sgrS_wt.
mutable notes: wild type.
mutable provenance: Escherichia coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: critical region for ptsG/SgrS interaction
sequence annotation: J23106
sequence annotation: E0840
sequence: https://synbiohub.org/public/igem/BBa_K581006_sequence/1

critical region for ptsG/SgrS interaction
type: SequenceAnnotation
persistent identity: annotation2152644
display id: annotation2152644
version: 1
top level: BBa_K581006
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K581006/annotation2152644/range2152644/1

https://synbiohub.org/public/igem/BBa_K581006/annotation2152644/range2152644/1
type: Range
persistent identity: range2152644
display id: range2152644
version: 1
direction: direction/forward
top level: BBa_K581006
start: 127
end: 132
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2152642
display id: annotation2152642
version: 1
top level: BBa_K581006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K581006/annotation2152642/range2152642/1

https://synbiohub.org/public/igem/BBa_K581006/annotation2152642/range2152642/1
type: Range
persistent identity: range2152642
display id: range2152642
version: 1
direction: direction/forward
top level: BBa_K581006
start: 1
end: 35
orientation: inline

E0840
type: SequenceAnnotation
persistent identity: annotation2152643
display id: annotation2152643
version: 1
top level: BBa_K581006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K581006/annotation2152643/range2152643/1

https://synbiohub.org/public/igem/BBa_K581006/annotation2152643/range2152643/1
type: Range
persistent identity: range2152643
display id: range2152643
version: 1
direction: direction/forward
top level: BBa_K581006
start: 147
end: 1003
orientation: inline

BBa_K577881
type: ComponentDefinition
persistent identity: BBa K577881
display id: BBa_K577881
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pC+AraC+pBad+GFPc
creator: Alberto Purwada
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
group u list: _748_
m user id: 0
owner id: 8431
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This reporter part is a combination of a constitutive promoter, AraC gene, a pBad promoter (inducible with arabinose), and a GFP gene. The whole part is flanked by the regular E, X and S, P restriction sites, so it can be approached with the usual restriction digest method. The purpose of this part is to provide the user with a simple, but complete, inducible genetic device. Adding arabinose with any concentration higher than 0.01 mM seems to cause a significant expression of the GFP gene. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. This part can be inserted in a cell line that does not contain any AraC in its genome, which is important if the user is not using Top 10 cells.
mutable notes: There is no specific design consideration.
mutable provenance: This part is obtained from ligating several biobricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13453
component: BBa_E0240
component: BBa_I13458
sequence annotation: BBa_I13458
sequence annotation: BBa_E0240
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K577881_sequence/1

BBa_I13453
type: Component
persistent identity: component2126995
display id: component2126995
version: 1
top level: BBa_K577881
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_E0240
type: Component
persistent identity: component2127006
display id: component2127006
version: 1
top level: BBa_K577881
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_I13458
type: Component
persistent identity: component2126994
display id: component2126994
version: 1
top level: BBa_K577881
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2126994
display id: annotation2126994
version: 1
top level: BBa_K577881
location: https://synbiohub.org/public/igem/BBa_K577881/annotation2126994/range2126994/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_K577881/annotation2126994/range2126994/1
type: Range
persistent identity: range2126994
display id: range2126994
version: 1
direction: direction/reverse
top level: BBa_K577881
start: 1
end: 1114
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2127006
display id: annotation2127006
version: 1
top level: BBa_K577881
location: https://synbiohub.org/public/igem/BBa_K577881/annotation2127006/range2127006/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K577881/annotation2127006/range2127006/1
type: Range
persistent identity: range2127006
display id: range2127006
version: 1
direction: direction/reverse
top level: BBa_K577881
start: 1261
end: 2136
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2126995
display id: annotation2126995
version: 1
top level: BBa_K577881
location: https://synbiohub.org/public/igem/BBa_K577881/annotation2126995/range2126995/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K577881/annotation2126995/range2126995/1
type: Range
persistent identity: range2126995
display id: range2126995
version: 1
direction: direction/reverse
top level: BBa_K577881
start: 1123
end: 1252
orientation: inline

BBa_K346000
type: ComponentDefinition
persistent identity: BBa K346000
display id: BBa_K346000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS(B0032)+T3 DNA-directed RNA polymerase
creator: Haoqian Zhang and Weiye Wang
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 0
owner id: 4411
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the coding region of MerR, which is a mercury responsive regulator from Tn21.
mutable notes: We cloned this part from plasmid NR1, which was a gift from Anne O. Summers.
mutable provenance: Tn21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: b0032
sequence annotation: t3 pol
sequence: https://synbiohub.org/public/igem/BBa_K346000_sequence/1

b0032
type: SequenceAnnotation
persistent identity: annotation2092860
display id: annotation2092860
version: 1
top level: BBa_K346000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K346000/annotation2092860/range2092860/1

https://synbiohub.org/public/igem/BBa_K346000/annotation2092860/range2092860/1
type: Range
persistent identity: range2092860
display id: range2092860
version: 1
direction: direction/forward
top level: BBa_K346000
start: 1
end: 14
orientation: inline

t3 pol
type: SequenceAnnotation
persistent identity: annotation2092859
display id: annotation2092859
version: 1
top level: BBa_K346000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346000/annotation2092859/range2092859/1

https://synbiohub.org/public/igem/BBa_K346000/annotation2092859/range2092859/1
type: Range
persistent identity: range2092859
display id: range2092859
version: 1
direction: direction/forward
top level: BBa_K346000
start: 19
end: 2674
orientation: inline

BBa_K584002
type: ComponentDefinition
persistent identity: BBa K584002
display id: BBa_K584002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-Lux hybrid + GFP generator
creator: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
created: 2011-08-15T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _755_
m user id: 0
owner id: 9879
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part was designed to test the functionality of the promotor region.
mutable notes: /
mutable provenance: /
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0240
component: BBa_K091100
sequence annotation: BBa_E0240
sequence annotation: BBa_K091100
sequence: https://synbiohub.org/public/igem/BBa_K584002_sequence/1

BBa_E0240
type: Component
persistent identity: component2125159
display id: component2125159
version: 1
top level: BBa_K584002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K091100
type: Component
persistent identity: component2125148
display id: component2125148
version: 1
top level: BBa_K584002
access: public
definition: https://synbiohub.org/public/igem/BBa_K091100/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2125159
display id: annotation2125159
version: 1
top level: BBa_K584002
location: https://synbiohub.org/public/igem/BBa_K584002/annotation2125159/range2125159/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K584002/annotation2125159/range2125159/1
type: Range
persistent identity: range2125159
display id: range2125159
version: 1
direction: direction/reverse
top level: BBa_K584002
start: 83
end: 958
orientation: inline

BBa_K091100
type: SequenceAnnotation
persistent identity: annotation2125148
display id: annotation2125148
version: 1
top level: BBa_K584002
location: https://synbiohub.org/public/igem/BBa_K584002/annotation2125148/range2125148/1
component: BBa_K091100

https://synbiohub.org/public/igem/BBa_K584002/annotation2125148/range2125148/1
type: Range
persistent identity: range2125148
display id: range2125148
version: 1
direction: direction/reverse
top level: BBa_K584002
start: 1
end: 74
orientation: inline

BBa_S04602
type: ComponentDefinition
persistent identity: BBa S04602
display id: BBa_S04602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:C0077
creator: Pinilla robin
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:14:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 8648
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_C0077
sequence annotation: BBa_C0077
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04602_sequence/1

BBa_B0034
type: Component
persistent identity: component2244646
display id: component2244646
version: 1
top level: BBa_S04602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0077
type: Component
persistent identity: component2244650
display id: component2244650
version: 1
top level: BBa_S04602
access: public
definition: https://synbiohub.org/public/igem/BBa_C0077/1

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation2244650
display id: annotation2244650
version: 1
top level: BBa_S04602
location: https://synbiohub.org/public/igem/BBa_S04602/annotation2244650/range2244650/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_S04602/annotation2244650/range2244650/1
type: Range
persistent identity: range2244650
display id: range2244650
version: 1
direction: direction/reverse
top level: BBa_S04602
start: 19
end: 805
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244646
display id: annotation2244646
version: 1
top level: BBa_S04602
location: https://synbiohub.org/public/igem/BBa_S04602/annotation2244646/range2244646/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04602/annotation2244646/range2244646/1
type: Range
persistent identity: range2244646
display id: range2244646
version: 1
direction: direction/reverse
top level: BBa_S04602
start: 1
end: 12
orientation: inline

BBa_K539651
type: ComponentDefinition
persistent identity: BBa K539651
display id: BBa_K539651
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated) + a RBS (B0032)+Alss with a RBS (B0030)+ilvC
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K539621
component: BBa_K539631
sequence annotation: BBa_K539631
sequence annotation: BBa_K539621
sequence: https://synbiohub.org/public/igem/BBa_K539651_sequence/1

BBa_K539621
type: Component
persistent identity: component2144114
display id: component2144114
version: 1
top level: BBa_K539651
access: public
definition: BBa_K539621

BBa_K539631
type: Component
persistent identity: component2144108
display id: component2144108
version: 1
top level: BBa_K539651
access: public
definition: https://synbiohub.org/public/igem/BBa_K539631/1

BBa_K539631
type: SequenceAnnotation
persistent identity: annotation2144108
display id: annotation2144108
version: 1
top level: BBa_K539651
location: https://synbiohub.org/public/igem/BBa_K539651/annotation2144108/range2144108/1
component: BBa_K539631

https://synbiohub.org/public/igem/BBa_K539651/annotation2144108/range2144108/1
type: Range
persistent identity: range2144108
display id: range2144108
version: 1
direction: direction/reverse
top level: BBa_K539651
start: 1
end: 1943
orientation: inline

BBa_K539621
type: SequenceAnnotation
persistent identity: annotation2144114
display id: annotation2144114
version: 1
top level: BBa_K539651
location: https://synbiohub.org/public/igem/BBa_K539651/annotation2144114/range2144114/1
component: BBa_K539621

https://synbiohub.org/public/igem/BBa_K539651/annotation2144114/range2144114/1
type: Range
persistent identity: range2144114
display id: range2144114
version: 1
direction: direction/reverse
top level: BBa_K539651
start: 1952
end: 3448
orientation: inline

BBa_K530025
type: ComponentDefinition
persistent identity: BBa K530025
display id: BBa_K530025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Vtc2
creator: Daniel Wolozny
created: 2011-09-02T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _696_
m user id: 0
owner id: 8447
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of GDP-L-Galactose to L-Galactose-1-P.
mutable notes: Part was constructed by ordering individual oligonucleotides. These oligos were then assembled through the use of CPEC and then further amplified with the outermost oligos as primers.
mutable provenance: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034532/
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GDP-L-Galactose Phosphorylase
sequence: https://synbiohub.org/public/igem/BBa_K530025_sequence/1

GDP-L-Galactose Phosphorylase
type: SequenceAnnotation
persistent identity: annotation2125823
display id: annotation2125823
version: 1
top level: BBa_K530025
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K530025/annotation2125823/range2125823/1

https://synbiohub.org/public/igem/BBa_K530025/annotation2125823/range2125823/1
type: Range
persistent identity: range2125823
display id: range2125823
version: 1
direction: direction/forward
top level: BBa_K530025
start: 1
end: 1293
orientation: inline

BBa_K819010
type: ComponentDefinition
persistent identity: BBa K819010
display id: BBa_K819010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium cheZ generator
creator: Xing Wenmin
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cheZ ligated to a medium promoter. This part express CheZ protein mediumly and will induce medium mobility in delta cheZ E.coli strain.
mutable notes: There is a point mutation at position 56 of CheZ coding sequence with have little effect to its function and the bases corresponding to stop codon is changed to bases coding a more frequently used stop codon.
mutable provenance: The promoter used here is from Partsregistry. The CheZ gene is from E.coli strain MG1655 by colony PCR and aligned with CheZ sequence at EcoGene database.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CheZ
sequence annotation: BBa_J23113
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K819010_sequence/1

CheZ
type: SequenceAnnotation
persistent identity: annotation2197375
display id: annotation2197375
version: 1
top level: BBa_K819010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819010/annotation2197375/range2197375/1

https://synbiohub.org/public/igem/BBa_K819010/annotation2197375/range2197375/1
type: Range
persistent identity: range2197375
display id: range2197375
version: 1
direction: direction/forward
top level: BBa_K819010
start: 62
end: 706
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2197349
display id: annotation2197349
version: 1
top level: BBa_K819010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819010/annotation2197349/range2197349/1

https://synbiohub.org/public/igem/BBa_K819010/annotation2197349/range2197349/1
type: Range
persistent identity: range2197349
display id: range2197349
version: 1
direction: direction/forward
top level: BBa_K819010
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2197359
display id: annotation2197359
version: 1
top level: BBa_K819010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K819010/annotation2197359/range2197359/1

https://synbiohub.org/public/igem/BBa_K819010/annotation2197359/range2197359/1
type: Range
persistent identity: range2197359
display id: range2197359
version: 1
direction: direction/forward
top level: BBa_K819010
start: 44
end: 55
orientation: inline

BBa_K652004
type: ComponentDefinition
persistent identity: BBa K652004
display id: BBa_K652004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-ZeAFP-Term
creator: Darren Zhu
created: 2011-08-25T11:00:00Z
modified: 2015-05-08T01:13:00Z
discontinued: false
dominant: false
group u list: _829_
m user id: 0
owner id: 6309
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains an RBS, the type III Zoarces elongatus antifreeze protein (ZeAFP), followed by a terminator.
mutable notes: N/A
mutable provenance: Generously provided to us by Peter Davies and Sherry Gauthier of Queen's University.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K652004_sequence/1

BBa_K523013
type: ComponentDefinition
persistent identity: BBa K523013
display id: BBa_K523013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac + INP-EYFP
creator: Sylvia Ispasanie, Mun Ching Lee
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) under the control of the Lac promoter.
mutable notes: Made using the BioSandwich protocol of Edinburgh 2011.
mutable provenance: Assembled from parts BBa_K523000 , BBa_K523008 , and BBa_K523012 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EYFP
sequence annotation: rbs
sequence annotation: Spacer
sequence annotation: INP-EYFP fusion
sequence annotation: Plac - LacZ
sequence annotation: ATG
sequence annotation: -10
sequence annotation: INP
sequence annotation: Spacer (Linker)
sequence annotation: TAATAA
sequence annotation: LacZ
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K523013_sequence/1

EYFP
type: SequenceAnnotation
persistent identity: annotation2125865
display id: annotation2125865
version: 1
top level: BBa_K523013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125865/range2125865/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125865/range2125865/1
type: Range
persistent identity: range2125865
display id: range2125865
version: 1
direction: direction/forward
top level: BBa_K523013
start: 1607
end: 2329
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129066
display id: annotation2129066
version: 1
top level: BBa_K523013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2129066/range2129066/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2129066/range2129066/1
type: Range
persistent identity: range2129066
display id: range2129066
version: 1
direction: direction/forward
top level: BBa_K523013
start: 348
end: 351
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2125859
display id: annotation2125859
version: 1
top level: BBa_K523013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125859/range2125859/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125859/range2125859/1
type: Range
persistent identity: range2125859
display id: range2125859
version: 1
direction: direction/forward
top level: BBa_K523013
start: 597
end: 625
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2125858
display id: annotation2125858
version: 1
top level: BBa_K523013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125858/range2125858/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125858/range2125858/1
type: Range
persistent identity: range2125858
display id: range2125858
version: 1
direction: direction/forward
top level: BBa_K523013
start: 1
end: 32
orientation: inline

INP-EYFP fusion
type: SequenceAnnotation
persistent identity: annotation2125867
display id: annotation2125867
version: 1
top level: BBa_K523013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125867/range2125867/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125867/range2125867/1
type: Range
persistent identity: range2125867
display id: range2125867
version: 1
direction: direction/forward
top level: BBa_K523013
start: 623
end: 2323
orientation: inline

Plac - LacZ
type: SequenceAnnotation
persistent identity: annotation2125860
display id: annotation2125860
version: 1
top level: BBa_K523013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125860/range2125860/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125860/range2125860/1
type: Range
persistent identity: range2125860
display id: range2125860
version: 1
direction: direction/forward
top level: BBa_K523013
start: 38
end: 589
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2125862
display id: annotation2125862
version: 1
top level: BBa_K523013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125862/range2125862/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125862/range2125862/1
type: Range
persistent identity: range2125862
display id: range2125862
version: 1
direction: direction/forward
top level: BBa_K523013
start: 623
end: 623
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2125869
display id: annotation2125869
version: 1
top level: BBa_K523013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125869/range2125869/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125869/range2125869/1
type: Range
persistent identity: range2125869
display id: range2125869
version: 1
direction: direction/forward
top level: BBa_K523013
start: 309
end: 314
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2125861
display id: annotation2125861
version: 1
top level: BBa_K523013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125861/range2125861/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125861/range2125861/1
type: Range
persistent identity: range2125861
display id: range2125861
version: 1
direction: direction/forward
top level: BBa_K523013
start: 611
end: 614
orientation: inline

INP
type: SequenceAnnotation
persistent identity: annotation2125863
display id: annotation2125863
version: 1
top level: BBa_K523013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125863/range2125863/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125863/range2125863/1
type: Range
persistent identity: range2125863
display id: range2125863
version: 1
direction: direction/forward
top level: BBa_K523013
start: 632
end: 1555
orientation: inline

Spacer (Linker)
type: SequenceAnnotation
persistent identity: annotation2125864
display id: annotation2125864
version: 1
top level: BBa_K523013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125864/range2125864/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125864/range2125864/1
type: Range
persistent identity: range2125864
display id: range2125864
version: 1
direction: direction/forward
top level: BBa_K523013
start: 1565
end: 1600
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2125868
display id: annotation2125868
version: 1
top level: BBa_K523013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125868/range2125868/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125868/range2125868/1
type: Range
persistent identity: range2125868
display id: range2125868
version: 1
direction: direction/forward
top level: BBa_K523013
start: 2324
end: 2324
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2125866
display id: annotation2125866
version: 1
top level: BBa_K523013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125866/range2125866/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125866/range2125866/1
type: Range
persistent identity: range2125866
display id: range2125866
version: 1
direction: direction/forward
top level: BBa_K523013
start: 2337
end: 2364
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2125871
display id: annotation2125871
version: 1
top level: BBa_K523013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125871/range2125871/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125871/range2125871/1
type: Range
persistent identity: range2125871
display id: range2125871
version: 1
direction: direction/forward
top level: BBa_K523013
start: 359
end: 589
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2125870
display id: annotation2125870
version: 1
top level: BBa_K523013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523013/annotation2125870/range2125870/1

https://synbiohub.org/public/igem/BBa_K523013/annotation2125870/range2125870/1
type: Range
persistent identity: range2125870
display id: range2125870
version: 1
direction: direction/forward
top level: BBa_K523013
start: 286
end: 291
orientation: inline

BBa_K546001
type: ComponentDefinition
persistent identity: BBa K546001
display id: BBa_K546001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled luxR with lux pR controlled AHL degrading enzyme AiiA(lva tag).
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. If 3OC6HSL is present in the cell, the autoinducer inactivation enzyme A (''aiiA''), with an LVA Tag, will inactivate the 3OC6HSL autoinducer molecules.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device.
mutable provenance: The lux p promoters originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_F2621
component: BBa_I0460
sequence annotation: BBa_F2621
sequence annotation: BBa_I0460
sequence: https://synbiohub.org/public/igem/BBa_K546001_sequence/1

BBa_F2621
type: Component
persistent identity: component2128233
display id: component2128233
version: 1
top level: BBa_K546001
access: public
definition: https://synbiohub.org/public/igem/BBa_F2621/1

BBa_I0460
type: Component
persistent identity: component2128248
display id: component2128248
version: 1
top level: BBa_K546001
access: public
definition: https://synbiohub.org/public/igem/BBa_I0460/1

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2128233
display id: annotation2128233
version: 1
top level: BBa_K546001
location: https://synbiohub.org/public/igem/BBa_K546001/annotation2128233/range2128233/1
component: BBa_F2621

https://synbiohub.org/public/igem/BBa_K546001/annotation2128233/range2128233/1
type: Range
persistent identity: range2128233
display id: range2128233
version: 1
direction: direction/reverse
top level: BBa_K546001
start: 1
end: 1158
orientation: inline

BBa_I0460
type: SequenceAnnotation
persistent identity: annotation2128248
display id: annotation2128248
version: 1
top level: BBa_K546001
location: https://synbiohub.org/public/igem/BBa_K546001/annotation2128248/range2128248/1
component: BBa_I0460

https://synbiohub.org/public/igem/BBa_K546001/annotation2128248/range2128248/1
type: Range
persistent identity: range2128248
display id: range2128248
version: 1
direction: direction/reverse
top level: BBa_K546001
start: 1167
end: 2135
orientation: inline

BBa_S04614
type: ComponentDefinition
persistent identity: BBa S04614
display id: BBa_S04614
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:K592002
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:14:42Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_K592002
sequence annotation: BBa_B0034
sequence annotation: BBa_K592002
sequence: https://synbiohub.org/public/igem/BBa_S04614_sequence/1

BBa_B0034
type: Component
persistent identity: component2136940
display id: component2136940
version: 1
top level: BBa_S04614
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592002
type: Component
persistent identity: component2136942
display id: component2136942
version: 1
top level: BBa_S04614
access: public
definition: BBa_K592002

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136940
display id: annotation2136940
version: 1
top level: BBa_S04614
location: https://synbiohub.org/public/igem/BBa_S04614/annotation2136940/range2136940/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04614/annotation2136940/range2136940/1
type: Range
persistent identity: range2136940
display id: range2136940
version: 1
direction: direction/reverse
top level: BBa_S04614
start: 1
end: 12
orientation: inline

BBa_K592002
type: SequenceAnnotation
persistent identity: annotation2136942
display id: annotation2136942
version: 1
top level: BBa_S04614
location: https://synbiohub.org/public/igem/BBa_S04614/annotation2136942/range2136942/1
component: BBa_K592002

https://synbiohub.org/public/igem/BBa_S04614/annotation2136942/range2136942/1
type: Range
persistent identity: range2136942
display id: range2136942
version: 1
direction: direction/reverse
top level: BBa_S04614
start: 19
end: 726
orientation: inline

BBa_K325100
type: ComponentDefinition
persistent identity: BBa K325100
display id: BBa_K325100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EPIC Firefly Luciferase and LRE P. Pyralis (E. coli optimised)
creator: Ben Reeve and Paul Masset
created: 2010-09-14T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a mutant luciferase that is very much brighter than wild-type. It is supplied in an operon with an enzyme (LRE) that regenerates its substrate. When supplied with luciferin and placed under a promoter it will emit light at a green wavelength. If D-cysteine is added the LRE will be able to regenerate the luciferin substrate.
mutable notes: It was ensured that no unwanted restriction sites were created during optimisation. A BamH1 site was added between the two genes.
mutable provenance: Codon optimised and synthesised by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: EPIC luciferase (P. pyralis)
sequence annotation: BamH1 site
sequence annotation: double stop codon
sequence annotation: rbs
sequence annotation: Luciferin regenerating enzyme (P.pyralis)
sequence: https://synbiohub.org/public/igem/BBa_K325100_sequence/1

EPIC luciferase (P. pyralis)
type: SequenceAnnotation
persistent identity: annotation2080161
display id: annotation2080161
version: 1
top level: BBa_K325100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325100/annotation2080161/range2080161/1

https://synbiohub.org/public/igem/BBa_K325100/annotation2080161/range2080161/1
type: Range
persistent identity: range2080161
display id: range2080161
version: 1
direction: direction/forward
top level: BBa_K325100
start: 971
end: 2620
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2080159
display id: annotation2080159
version: 1
top level: BBa_K325100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325100/annotation2080159/range2080159/1

https://synbiohub.org/public/igem/BBa_K325100/annotation2080159/range2080159/1
type: Range
persistent identity: range2080159
display id: range2080159
version: 1
direction: direction/forward
top level: BBa_K325100
start: 948
end: 953
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2080158
display id: annotation2080158
version: 1
top level: BBa_K325100
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325100/annotation2080158/range2080158/1

https://synbiohub.org/public/igem/BBa_K325100/annotation2080158/range2080158/1
type: Range
persistent identity: range2080158
display id: range2080158
version: 1
direction: direction/forward
top level: BBa_K325100
start: 942
end: 947
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2080160
display id: annotation2080160
version: 1
top level: BBa_K325100
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325100/annotation2080160/range2080160/1

https://synbiohub.org/public/igem/BBa_K325100/annotation2080160/range2080160/1
type: Range
persistent identity: range2080160
display id: range2080160
version: 1
direction: direction/forward
top level: BBa_K325100
start: 954
end: 970
orientation: inline

Luciferin regenerating enzyme (P.pyralis)
type: SequenceAnnotation
persistent identity: annotation2080157
display id: annotation2080157
version: 1
top level: BBa_K325100
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325100/annotation2080157/range2080157/1

https://synbiohub.org/public/igem/BBa_K325100/annotation2080157/range2080157/1
type: Range
persistent identity: range2080157
display id: range2080157
version: 1
direction: direction/forward
top level: BBa_K325100
start: 18
end: 941
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2080156
display id: annotation2080156
version: 1
top level: BBa_K325100
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325100/annotation2080156/range2080156/1

https://synbiohub.org/public/igem/BBa_K325100/annotation2080156/range2080156/1
type: Range
persistent identity: range2080156
display id: range2080156
version: 1
direction: direction/forward
top level: BBa_K325100
start: 1
end: 17
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2080162
display id: annotation2080162
version: 1
top level: BBa_K325100
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325100/annotation2080162/range2080162/1

https://synbiohub.org/public/igem/BBa_K325100/annotation2080162/range2080162/1
type: Range
persistent identity: range2080162
display id: range2080162
version: 1
direction: direction/forward
top level: BBa_K325100
start: 2621
end: 2626
orientation: inline

BBa_K741006
type: ComponentDefinition
persistent identity: BBa K741006
display id: BBa_K741006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-crogfp-T
creator: Wuyang Chen
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: It is the fusion protein of Cro and GFP with the RBS and the terminator
mutable notes: TBD
mutable provenance: BBa_B0034 BBa_K741005 BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K741005
sequence annotation: BBa_B0034
sequence annotation: BBa_K741005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K741006_sequence/1

BBa_B0034
type: Component
persistent identity: component2180123
display id: component2180123
version: 1
top level: BBa_K741006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2180131
display id: component2180131
version: 1
top level: BBa_K741006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K741005
type: Component
persistent identity: component2180124
display id: component2180124
version: 1
top level: BBa_K741006
access: public
definition: https://synbiohub.org/public/igem/BBa_K741005/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2180123
display id: annotation2180123
version: 1
top level: BBa_K741006
location: https://synbiohub.org/public/igem/BBa_K741006/annotation2180123/range2180123/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K741006/annotation2180123/range2180123/1
type: Range
persistent identity: range2180123
display id: range2180123
version: 1
direction: direction/reverse
top level: BBa_K741006
start: 1
end: 12
orientation: inline

BBa_K741005
type: SequenceAnnotation
persistent identity: annotation2180124
display id: annotation2180124
version: 1
top level: BBa_K741006
location: https://synbiohub.org/public/igem/BBa_K741006/annotation2180124/range2180124/1
component: BBa_K741005

https://synbiohub.org/public/igem/BBa_K741006/annotation2180124/range2180124/1
type: Range
persistent identity: range2180124
display id: range2180124
version: 1
direction: direction/reverse
top level: BBa_K741006
start: 19
end: 951
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2180131
display id: annotation2180131
version: 1
top level: BBa_K741006
location: https://synbiohub.org/public/igem/BBa_K741006/annotation2180131/range2180131/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K741006/annotation2180131/range2180131/1
type: Range
persistent identity: range2180131
display id: range2180131
version: 1
direction: direction/reverse
top level: BBa_K741006
start: 960
end: 1088
orientation: inline

BBa_K642000
type: ComponentDefinition
persistent identity: BBa K642000
display id: BBa_K642000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressor tagged with yBFP
creator: Wilson Lam, Matthew Orton
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _818_
m user id: 0
owner id: 9288
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a tetracycline responsive repressor that is tagged with yeast codon optimized BFP.
mutable notes: Needed the BFP to be codon optimized for yeast
mutable provenance: BFP was synthesized Tetr is from the Registry of Parts: BBa_K319046
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TetR ORF
sequence annotation: xbaI/speI mixed site
sequence annotation: BFP ORF
sequence: https://synbiohub.org/public/igem/BBa_K642000_sequence/1

TetR ORF
type: SequenceAnnotation
persistent identity: annotation2138586
display id: annotation2138586
version: 1
top level: BBa_K642000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K642000/annotation2138586/range2138586/1

https://synbiohub.org/public/igem/BBa_K642000/annotation2138586/range2138586/1
type: Range
persistent identity: range2138586
display id: range2138586
version: 1
direction: direction/forward
top level: BBa_K642000
start: 1
end: 621
orientation: inline

xbaI/speI mixed site
type: SequenceAnnotation
persistent identity: annotation2138080
display id: annotation2138080
version: 1
top level: BBa_K642000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K642000/annotation2138080/range2138080/1

https://synbiohub.org/public/igem/BBa_K642000/annotation2138080/range2138080/1
type: Range
persistent identity: range2138080
display id: range2138080
version: 1
direction: direction/forward
top level: BBa_K642000
start: 622
end: 627
orientation: inline

BFP ORF
type: SequenceAnnotation
persistent identity: annotation2138587
display id: annotation2138587
version: 1
top level: BBa_K642000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K642000/annotation2138587/range2138587/1

https://synbiohub.org/public/igem/BBa_K642000/annotation2138587/range2138587/1
type: Range
persistent identity: range2138587
display id: range2138587
version: 1
direction: direction/forward
top level: BBa_K642000
start: 628
end: 1326
orientation: inline

BBa_K823014
type: ComponentDefinition
persistent identity: BBa K823014
display id: BBa_K823014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23118
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23118 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: J23118
sequence: https://synbiohub.org/public/igem/BBa_K823014_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182548
display id: annotation2182548
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1
type: Range
persistent identity: range2182548
display id: range2182548
version: 1
direction: direction/forward
top level: BBa_K823014
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182547
display id: annotation2182547
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1
type: Range
persistent identity: range2182547
display id: range2182547
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 6
orientation: inline

J23118
type: SequenceAnnotation
persistent identity: annotation2182546
display id: annotation2182546
version: 1
top level: BBa_K823014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1
type: Range
persistent identity: range2182546
display id: range2182546
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 35
orientation: inline

pCaiF
type: ComponentDefinition
persistent identity: BBa K398326
display id: BBa_K398326
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of the CaiF protein
creator: Hugo Federico Cueto Rojas
created: 2010-06-13T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6320
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Promoter region from E.coli containing the binding site for the global regulator Crp.
mutable notes: None
mutable provenance: Genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pCaiF
sequence annotation: Crp-cAMP
sequence: https://synbiohub.org/public/igem/BBa_K398326_sequence/1

pCaiF
type: SequenceAnnotation
persistent identity: annotation2090459
display id: annotation2090459
version: 1
top level: pCaiF
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K398326/annotation2090459/range2090459/1

https://synbiohub.org/public/igem/BBa_K398326/annotation2090459/range2090459/1
type: Range
persistent identity: range2090459
display id: range2090459
version: 1
direction: direction/forward
top level: pCaiF
start: 16
end: 45
orientation: inline

Crp-cAMP
type: SequenceAnnotation
persistent identity: annotation2090458
display id: annotation2090458
version: 1
top level: pCaiF
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K398326/annotation2090458/range2090458/1

https://synbiohub.org/public/igem/BBa_K398326/annotation2090458/range2090458/1
type: Range
persistent identity: range2090458
display id: range2090458
version: 1
direction: direction/forward
top level: pCaiF
start: 1
end: 21
orientation: inline

BBa_K539119
type: ComponentDefinition
persistent identity: BBa K539119
display id: BBa_K539119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtY (lycopene cyclase)
creator: Yang, Yi-Pei
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. The native ribosome binding site is included. This is part of the carotenoid biosynthesis pathway, converting lycopene to beta-carotene.
mutable notes: This part is from previous team, but we find a point mutation in the sequence. We have correct the mutation, and this is still an available biobrick.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K118008
sequence annotation: crtY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K539119_sequence/1

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2131424
display id: annotation2131424
version: 1
top level: BBa_K539119
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K539119/annotation2131424/range2131424/1

https://synbiohub.org/public/igem/BBa_K539119/annotation2131424/range2131424/1
type: Range
persistent identity: range2131424
display id: range2131424
version: 1
direction: direction/reverse
top level: BBa_K539119
start: 1
end: 1152
orientation: inline

crtY coding sequence
type: SequenceAnnotation
persistent identity: annotation2131423
display id: annotation2131423
version: 1
top level: BBa_K539119
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K539119/annotation2131423/range2131423/1

https://synbiohub.org/public/igem/BBa_K539119/annotation2131423/range2131423/1
type: Range
persistent identity: range2131423
display id: range2131423
version: 1
direction: direction/forward
top level: BBa_K539119
start: 1
end: 1149
orientation: inline

BBa_K592006
type: ComponentDefinition
persistent identity: BBa K592006
display id: BBa_K592006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FixK2 promoter
creator: Lei Sun
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
mutable notes: The sequence information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
mutable provenance: FixK2 promoter omes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FixK2 promoter
sequence: https://synbiohub.org/public/igem/BBa_K592006_sequence/1

FixK2 promoter
type: SequenceAnnotation
persistent identity: annotation2130179
display id: annotation2130179
version: 1
top level: BBa_K592006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1

https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
type: Range
persistent identity: range2130179
display id: range2130179
version: 1
direction: direction/forward
top level: BBa_K592006
start: 1
end: 250
orientation: inline

BBa_K325903
type: ComponentDefinition
persistent identity: BBa K325903
display id: BBa_K325903
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxEG V. Fischeri (E. coli optimised)
creator: Peter Emmrich and Anja Hohmann
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: rbs
sequence annotation: luxE
sequence annotation: terminator
sequence annotation: luxG
sequence: https://synbiohub.org/public/igem/BBa_K325903_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2107847
display id: annotation2107847
version: 1
top level: BBa_K325903
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325903/annotation2107847/range2107847/1

https://synbiohub.org/public/igem/BBa_K325903/annotation2107847/range2107847/1
type: Range
persistent identity: range2107847
display id: range2107847
version: 1
direction: direction/forward
top level: BBa_K325903
start: 1155
end: 1171
orientation: inline

luxE
type: SequenceAnnotation
persistent identity: annotation2107848
display id: annotation2107848
version: 1
top level: BBa_K325903
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325903/annotation2107848/range2107848/1

https://synbiohub.org/public/igem/BBa_K325903/annotation2107848/range2107848/1
type: Range
persistent identity: range2107848
display id: range2107848
version: 1
direction: direction/forward
top level: BBa_K325903
start: 18
end: 1154
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2107851
display id: annotation2107851
version: 1
top level: BBa_K325903
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K325903/annotation2107851/range2107851/1

https://synbiohub.org/public/igem/BBa_K325903/annotation2107851/range2107851/1
type: Range
persistent identity: range2107851
display id: range2107851
version: 1
direction: direction/forward
top level: BBa_K325903
start: 1883
end: 1921
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2107845
display id: annotation2107845
version: 1
top level: BBa_K325903
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325903/annotation2107845/range2107845/1

https://synbiohub.org/public/igem/BBa_K325903/annotation2107845/range2107845/1
type: Range
persistent identity: range2107845
display id: range2107845
version: 1
direction: direction/forward
top level: BBa_K325903
start: 1
end: 17
orientation: inline

luxG
type: SequenceAnnotation
persistent identity: annotation2107850
display id: annotation2107850
version: 1
top level: BBa_K325903
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325903/annotation2107850/range2107850/1

https://synbiohub.org/public/igem/BBa_K325903/annotation2107850/range2107850/1
type: Range
persistent identity: range2107850
display id: range2107850
version: 1
direction: direction/forward
top level: BBa_K325903
start: 1172
end: 1882
orientation: inline

AraC_14_3C
type: ComponentDefinition
persistent identity: BBa K627011
display id: BBa_K627011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease
creator: iGEM11 Potsdam_Bioware
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _799_
m user id: 0
owner id: 10585
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: AraC
sequence annotation: HRV 14 3C proteas
sequence annotation: AraO1
sequence annotation: AraO2
sequence annotation: Ara1|2
sequence annotation: HRV 14_3C protease
sequence annotation: T7 RBS
sequence annotation: Ara
sequence annotation: CAP
sequence: https://synbiohub.org/public/igem/BBa_K627011_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation2136678
display id: annotation2136678
version: 1
top level: AraC_14_3C
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136678/range2136678/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136678/range2136678/1
type: Range
persistent identity: range2136678
display id: range2136678
version: 1
direction: direction/reverse
top level: AraC_14_3C
start: 1
end: 3
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2136680
display id: annotation2136680
version: 1
top level: AraC_14_3C
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136680/range2136680/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136680/range2136680/1
type: Range
persistent identity: range2136680
display id: range2136680
version: 1
direction: direction/reverse
top level: AraC_14_3C
start: 877
end: 879
orientation: inline

HRV 14 3C proteas
type: SequenceAnnotation
persistent identity: annotation2136687
display id: annotation2136687
version: 1
top level: AraC_14_3C
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136687/range2136687/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136687/range2136687/1
type: Range
persistent identity: range2136687
display id: range2136687
version: 1
direction: direction/forward
top level: AraC_14_3C
start: 1245
end: 1247
orientation: inline

AraO1
type: SequenceAnnotation
persistent identity: annotation2136681
display id: annotation2136681
version: 1
top level: AraC_14_3C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136681/range2136681/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136681/range2136681/1
type: Range
persistent identity: range2136681
display id: range2136681
version: 1
direction: direction/forward
top level: AraC_14_3C
start: 909
end: 924
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2136679
display id: annotation2136679
version: 1
top level: AraC_14_3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136679/range2136679/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136679/range2136679/1
type: Range
persistent identity: range2136679
display id: range2136679
version: 1
direction: direction/reverse
top level: AraC_14_3C
start: 1
end: 879
orientation: inline

AraO2
type: SequenceAnnotation
persistent identity: annotation2136682
display id: annotation2136682
version: 1
top level: AraC_14_3C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136682/range2136682/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136682/range2136682/1
type: Range
persistent identity: range2136682
display id: range2136682
version: 1
direction: direction/forward
top level: AraC_14_3C
start: 1066
end: 1077
orientation: inline

Ara1|2
type: SequenceAnnotation
persistent identity: annotation2136684
display id: annotation2136684
version: 1
top level: AraC_14_3C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136684/range2136684/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136684/range2136684/1
type: Range
persistent identity: range2136684
display id: range2136684
version: 1
direction: direction/forward
top level: AraC_14_3C
start: 1118
end: 1156
orientation: inline

HRV 14_3C protease
type: SequenceAnnotation
persistent identity: annotation2136688
display id: annotation2136688
version: 1
top level: AraC_14_3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136688/range2136688/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136688/range2136688/1
type: Range
persistent identity: range2136688
display id: range2136688
version: 1
direction: direction/forward
top level: AraC_14_3C
start: 1245
end: 1793
orientation: inline

T7 RBS
type: SequenceAnnotation
persistent identity: annotation2136686
display id: annotation2136686
version: 1
top level: AraC_14_3C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136686/range2136686/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136686/range2136686/1
type: Range
persistent identity: range2136686
display id: range2136686
version: 1
direction: direction/forward
top level: AraC_14_3C
start: 1222
end: 1238
orientation: inline

Ara
type: SequenceAnnotation
persistent identity: annotation2136685
display id: annotation2136685
version: 1
top level: AraC_14_3C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136685/range2136685/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136685/range2136685/1
type: Range
persistent identity: range2136685
display id: range2136685
version: 1
direction: direction/forward
top level: AraC_14_3C
start: 1153
end: 1185
orientation: inline

CAP
type: SequenceAnnotation
persistent identity: annotation2136683
display id: annotation2136683
version: 1
top level: AraC_14_3C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K627011/annotation2136683/range2136683/1

https://synbiohub.org/public/igem/BBa_K627011/annotation2136683/range2136683/1
type: Range
persistent identity: range2136683
display id: range2136683
version: 1
direction: direction/forward
top level: AraC_14_3C
start: 1108
end: 1121
orientation: inline

BBa_K934026
type: ComponentDefinition
persistent identity: BBa K934026
display id: BBa_K934026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux-LacI
creator: Naoki Tsukuda
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1199_
m user id: 0
owner id: 14032
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI generator which is activated by LuxR/3OC6HSL complex
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I732820
component: BBa_R0062
sequence annotation: BBa_I732820
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K934026_sequence/1

BBa_I732820
type: Component
persistent identity: component2184349
display id: component2184349
version: 1
top level: BBa_K934026
access: public
definition: https://synbiohub.org/public/igem/BBa_I732820/1

BBa_R0062
type: Component
persistent identity: component2184335
display id: component2184335
version: 1
top level: BBa_K934026
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_I732820
type: SequenceAnnotation
persistent identity: annotation2184349
display id: annotation2184349
version: 1
top level: BBa_K934026
location: https://synbiohub.org/public/igem/BBa_K934026/annotation2184349/range2184349/1
component: BBa_I732820

https://synbiohub.org/public/igem/BBa_K934026/annotation2184349/range2184349/1
type: Range
persistent identity: range2184349
display id: range2184349
version: 1
direction: direction/reverse
top level: BBa_K934026
start: 64
end: 1304
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2184335
display id: annotation2184335
version: 1
top level: BBa_K934026
location: https://synbiohub.org/public/igem/BBa_K934026/annotation2184335/range2184335/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K934026/annotation2184335/range2184335/1
type: Range
persistent identity: range2184335
display id: range2184335
version: 1
direction: direction/forward
top level: BBa_K934026
start: 1
end: 55
orientation: inline

BBa_K539627
type: ComponentDefinition
persistent identity: BBa K539627
display id: BBa_K539627
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a RBS (B0032)+Alss
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K539713
component: BBa_B0032
sequence annotation: BBa_K539713
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K539627_sequence/1

BBa_K539713
type: Component
persistent identity: component2144007
display id: component2144007
version: 1
top level: BBa_K539627
access: public
definition: https://synbiohub.org/public/igem/BBa_K539713/1

BBa_B0032
type: Component
persistent identity: component2144004
display id: component2144004
version: 1
top level: BBa_K539627
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K539713
type: SequenceAnnotation
persistent identity: annotation2144007
display id: annotation2144007
version: 1
top level: BBa_K539627
location: https://synbiohub.org/public/igem/BBa_K539627/annotation2144007/range2144007/1
component: BBa_K539713

https://synbiohub.org/public/igem/BBa_K539627/annotation2144007/range2144007/1
type: Range
persistent identity: range2144007
display id: range2144007
version: 1
direction: direction/reverse
top level: BBa_K539627
start: 20
end: 1735
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2144004
display id: annotation2144004
version: 1
top level: BBa_K539627
location: https://synbiohub.org/public/igem/BBa_K539627/annotation2144004/range2144004/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K539627/annotation2144004/range2144004/1
type: Range
persistent identity: range2144004
display id: range2144004
version: 1
direction: direction/forward
top level: BBa_K539627
start: 1
end: 13
orientation: inline

BBa_K538104
type: ComponentDefinition
persistent identity: BBa K538104
display id: BBa_K538104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + CspC
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K538004
sequence annotation: BBa_K538004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538104_sequence/1

BBa_B0034
type: Component
persistent identity: component2133904
display id: component2133904
version: 1
top level: BBa_K538104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538004
type: Component
persistent identity: component2133910
display id: component2133910
version: 1
top level: BBa_K538104
access: public
definition: CspC

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2133910
display id: annotation2133910
version: 1
top level: BBa_K538104
location: https://synbiohub.org/public/igem/BBa_K538104/annotation2133910/range2133910/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538104/annotation2133910/range2133910/1
type: Range
persistent identity: range2133910
display id: range2133910
version: 1
direction: direction/reverse
top level: BBa_K538104
start: 19
end: 213
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133904
display id: annotation2133904
version: 1
top level: BBa_K538104
location: https://synbiohub.org/public/igem/BBa_K538104/annotation2133904/range2133904/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538104/annotation2133904/range2133904/1
type: Range
persistent identity: range2133904
display id: range2133904
version: 1
direction: direction/reverse
top level: BBa_K538104
start: 1
end: 12
orientation: inline

BBa_K607035
type: ComponentDefinition
persistent identity: BBa K607035
display id: BBa_K607035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pUC18 replication origin
creator: Christoph Wittich
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
group u list: _779_
m user id: 0
owner id: 8902
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K607035 is a pUC18 origin of replication. [edit] Usage and Biology BBa_I50032 yields vectors with an expected copy number of 30-40 per cell.
mutable notes: none
mutable provenance: Obtained by PCR on a plasmid prep of a pUC 18 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K607035_sequence/1

BBa_K805016
type: ComponentDefinition
persistent identity: BBa K805016
display id: BBa_K805016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxI
creator: Han Jiapeng
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1062_
m user id: 0
owner id: 11895
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: test
mutable notes: test
mutable provenance: no
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_C0061
sequence annotation: BBa_C0061
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K805016_sequence/1

BBa_B0015
type: Component
persistent identity: component2251176
display id: component2251176
version: 1
top level: BBa_K805016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2251165
display id: component2251165
version: 1
top level: BBa_K805016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0061
type: Component
persistent identity: component2251167
display id: component2251167
version: 1
top level: BBa_K805016
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2251167
display id: annotation2251167
version: 1
top level: BBa_K805016
location: https://synbiohub.org/public/igem/BBa_K805016/annotation2251167/range2251167/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K805016/annotation2251167/range2251167/1
type: Range
persistent identity: range2251167
display id: range2251167
version: 1
direction: direction/forward
top level: BBa_K805016
start: 19
end: 636
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251176
display id: annotation2251176
version: 1
top level: BBa_K805016
location: https://synbiohub.org/public/igem/BBa_K805016/annotation2251176/range2251176/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K805016/annotation2251176/range2251176/1
type: Range
persistent identity: range2251176
display id: range2251176
version: 1
direction: direction/reverse
top level: BBa_K805016
start: 670
end: 798
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2251165
display id: annotation2251165
version: 1
top level: BBa_K805016
location: https://synbiohub.org/public/igem/BBa_K805016/annotation2251165/range2251165/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K805016/annotation2251165/range2251165/1
type: Range
persistent identity: range2251165
display id: range2251165
version: 1
direction: direction/reverse
top level: BBa_K805016
start: 1
end: 12
orientation: inline

BBa_K515102
type: ComponentDefinition
persistent identity: BBa K515102
display id: BBa_K515102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 promoter - PA2652
creator: Atipat Patharagulpong
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: A composite part comprising of a J23100 promotor and PA2652 translational unit encoding extracellular chemoreceptor
mutable notes: -
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K515002
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K515002
sequence: https://synbiohub.org/public/igem/BBa_K515102_sequence/1

BBa_K515002
type: Component
persistent identity: component2127194
display id: component2127194
version: 1
top level: BBa_K515102
access: public
definition: https://synbiohub.org/public/igem/BBa_K515002/1

BBa_J23100
type: Component
persistent identity: component2127190
display id: component2127190
version: 1
top level: BBa_K515102
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2127190
display id: annotation2127190
version: 1
top level: BBa_K515102
location: https://synbiohub.org/public/igem/BBa_K515102/annotation2127190/range2127190/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K515102/annotation2127190/range2127190/1
type: Range
persistent identity: range2127190
display id: range2127190
version: 1
direction: direction/reverse
top level: BBa_K515102
start: 1
end: 35
orientation: inline

BBa_K515002
type: SequenceAnnotation
persistent identity: annotation2127194
display id: annotation2127194
version: 1
top level: BBa_K515102
location: https://synbiohub.org/public/igem/BBa_K515102/annotation2127194/range2127194/1
component: BBa_K515002

https://synbiohub.org/public/igem/BBa_K515102/annotation2127194/range2127194/1
type: Range
persistent identity: range2127194
display id: range2127194
version: 1
direction: direction/reverse
top level: BBa_K515102
start: 36
end: 1759
orientation: inline

BBa_K539672
type: ComponentDefinition
persistent identity: BBa K539672
display id: BBa_K539672
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)+RBS+Alss with a RBS (B0032)+ilvC+a RBS (B0032)+ilvD
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K539631
component: BBa_K539641
sequence annotation: BBa_K539631
sequence annotation: BBa_K539641
sequence: https://synbiohub.org/public/igem/BBa_K539672_sequence/1

BBa_K539631
type: Component
persistent identity: component2144237
display id: component2144237
version: 1
top level: BBa_K539672
access: public
definition: https://synbiohub.org/public/igem/BBa_K539631/1

BBa_K539641
type: Component
persistent identity: component2144250
display id: component2144250
version: 1
top level: BBa_K539672
access: public
definition: BBa_K539641

BBa_K539631
type: SequenceAnnotation
persistent identity: annotation2144237
display id: annotation2144237
version: 1
top level: BBa_K539672
location: https://synbiohub.org/public/igem/BBa_K539672/annotation2144237/range2144237/1
component: BBa_K539631

https://synbiohub.org/public/igem/BBa_K539672/annotation2144237/range2144237/1
type: Range
persistent identity: range2144237
display id: range2144237
version: 1
direction: direction/reverse
top level: BBa_K539672
start: 1
end: 1943
orientation: inline

BBa_K539641
type: SequenceAnnotation
persistent identity: annotation2144250
display id: annotation2144250
version: 1
top level: BBa_K539672
location: https://synbiohub.org/public/igem/BBa_K539672/annotation2144250/range2144250/1
component: BBa_K539641

https://synbiohub.org/public/igem/BBa_K539672/annotation2144250/range2144250/1
type: Range
persistent identity: range2144250
display id: range2144250
version: 1
direction: direction/reverse
top level: BBa_K539672
start: 1952
end: 5324
orientation: inline

BBa_K861090
type: ComponentDefinition
persistent identity: BBa K861090
display id: BBa_K861090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YhjH Gene From E.coli str. K12
creator: Kuanwei Sheng
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:35Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1121_
m user id: 0
owner id: 11007
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: YhjH protein contain a EAL domain to catalyze c-di-GMP into GMP. c-di-GMP is a magic molecule that can activates bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. Thus, the expression of YhjH gene can make the bacteria more motile.
mutable notes: None
mutable provenance: E.coli DH5α
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K861090_sequence/1

BBa_K568004
type: ComponentDefinition
persistent identity: BBa K568004
display id: BBa_K568004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Optogenetical AND-Gate blue light - without first promoter - choose your first input
creator: Team TU Munich 2011
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _736_
m user id: 0
owner id: 10022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tbd
mutable notes: tbd
mutable provenance: tbd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K238013
component: BBa_K228001
component: BBa_K228000
component: BBa_B0015
component: BBa_J44001
sequence annotation: BBa_K228000
sequence annotation: BBa_K228001
sequence annotation: BBa_B0015
sequence annotation: BBa_K238013
sequence annotation: BBa_J44001
sequence: https://synbiohub.org/public/igem/BBa_K568004_sequence/1

BBa_K238013
type: Component
persistent identity: component2128029
display id: component2128029
version: 1
top level: BBa_K568004
access: public
definition: https://synbiohub.org/public/igem/BBa_K238013/1

BBa_K228001
type: Component
persistent identity: component2128015
display id: component2128015
version: 1
top level: BBa_K568004
access: public
definition: https://synbiohub.org/public/igem/BBa_K228001/1

BBa_K228000
type: Component
persistent identity: component2128033
display id: component2128033
version: 1
top level: BBa_K568004
access: public
definition: https://synbiohub.org/public/igem/BBa_K228000/1

BBa_B0015
type: Component
persistent identity: component2128022
display id: component2128022
version: 1
top level: BBa_K568004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J44001
type: Component
persistent identity: component2128031
display id: component2128031
version: 1
top level: BBa_K568004
access: public
definition: https://synbiohub.org/public/igem/BBa_J44001/1

BBa_K228000
type: SequenceAnnotation
persistent identity: annotation2128033
display id: annotation2128033
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128033/range2128033/1
component: BBa_K228000

https://synbiohub.org/public/igem/BBa_K568004/annotation2128033/range2128033/1
type: Range
persistent identity: range2128033
display id: range2128033
version: 1
direction: direction/reverse
top level: BBa_K568004
start: 397
end: 3069
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2128015
display id: annotation2128015
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128015/range2128015/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K568004/annotation2128015/range2128015/1
type: Range
persistent identity: range2128015
display id: range2128015
version: 1
direction: direction/reverse
top level: BBa_K568004
start: 1
end: 136
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2128022
display id: annotation2128022
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128022/range2128022/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K568004/annotation2128022/range2128022/1
type: Range
persistent identity: range2128022
display id: range2128022
version: 1
direction: direction/reverse
top level: BBa_K568004
start: 145
end: 273
orientation: inline

BBa_K238013
type: SequenceAnnotation
persistent identity: annotation2128029
display id: annotation2128029
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128029/range2128029/1
component: BBa_K238013

https://synbiohub.org/public/igem/BBa_K568004/annotation2128029/range2128029/1
type: Range
persistent identity: range2128029
display id: range2128029
version: 1
direction: direction/reverse
top level: BBa_K568004
start: 282
end: 367
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2128031
display id: annotation2128031
version: 1
top level: BBa_K568004
location: https://synbiohub.org/public/igem/BBa_K568004/annotation2128031/range2128031/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_K568004/annotation2128031/range2128031/1
type: Range
persistent identity: range2128031
display id: range2128031
version: 1
direction: direction/reverse
top level: BBa_K568004
start: 376
end: 390
orientation: inline

B-M
type: ComponentDefinition
persistent identity: BBa K774000
display id: BBa_K774000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacterial-Mammalian Promoter: BBaK216005 + BHN + CArG promoter sequence E9-ns2
creator: NRP-UEA-Norwich
created: 2012-06-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1026_
m user id: 0
owner id: 11706
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: A hybrid of the PyeaR promoter (BBa_K216005) and the E9-ns2 CArG promoter (Ref:1). Two versions will be synthesised, with the promoters altering their position in relation to the 5???-end of the sequence.
mutable notes: To provide additional restriction enzyme sites that may become useful during later cloning steps, BamHI, HindIII and NdeI have been added between the 2 promoters. It is envisaged that any open reading frame (e.g. RFP or GFP) will be cloned ???downstream??? (i.e. at the 3???-end) of these promoter sequences.
mutable provenance: PyeaR promoter (BBa_K216005) and the E9-ns2 CArG promoter (Ref:1) Ref1: Scott, S.D., Joiner, M.C. & Marples, B., 2002. Optimizing radiation-responsive gene promoters for radiogenetic cancer therapy. Gene therapy, 9(20), p.1396-402. Available at: http://www.ncbi.nlm.nih.gov/pubmed/12365005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CArG promoter sequence E9-ns2
sequence annotation: BBaK216005
sequence annotation: BHN
sequence: https://synbiohub.org/public/igem/BBa_K774000_sequence/1

CArG promoter sequence E9-ns2
type: SequenceAnnotation
persistent identity: annotation2177197
display id: annotation2177197
version: 1
top level: B-M
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K774000/annotation2177197/range2177197/1

https://synbiohub.org/public/igem/BBa_K774000/annotation2177197/range2177197/1
type: Range
persistent identity: range2177197
display id: range2177197
version: 1
direction: direction/forward
top level: B-M
start: 120
end: 208
orientation: inline

BBaK216005
type: SequenceAnnotation
persistent identity: annotation2177195
display id: annotation2177195
version: 1
top level: B-M
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K774000/annotation2177195/range2177195/1

https://synbiohub.org/public/igem/BBa_K774000/annotation2177195/range2177195/1
type: Range
persistent identity: range2177195
display id: range2177195
version: 1
direction: direction/forward
top level: B-M
start: 1
end: 100
orientation: inline

BHN
type: SequenceAnnotation
persistent identity: annotation2177196
display id: annotation2177196
version: 1
top level: B-M
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K774000/annotation2177196/range2177196/1

https://synbiohub.org/public/igem/BBa_K774000/annotation2177196/range2177196/1
type: Range
persistent identity: range2177196
display id: range2177196
version: 1
direction: direction/forward
top level: B-M
start: 101
end: 119
orientation: inline

BBa_K575036
type: ComponentDefinition
persistent identity: BBa K575036
display id: BBa_K575036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP + Constitutive Promoter + RBS (B0030) + RhlR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of RhlR combined with receptor with RhlP.
mutable notes: put the constitutive promoter after the production of RhlR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575013
component: BBa_K575006
sequence annotation: BBa_K575013
sequence annotation: BBa_K575006
sequence: https://synbiohub.org/public/igem/BBa_K575036_sequence/1

BBa_K575013
type: Component
persistent identity: component2138433
display id: component2138433
version: 1
top level: BBa_K575036
access: public
definition: BBa_K575013

BBa_K575006
type: Component
persistent identity: component2138439
display id: component2138439
version: 1
top level: BBa_K575036
access: public
definition: https://synbiohub.org/public/igem/BBa_K575006/1

BBa_K575013
type: SequenceAnnotation
persistent identity: annotation2138433
display id: annotation2138433
version: 1
top level: BBa_K575036
location: https://synbiohub.org/public/igem/BBa_K575036/annotation2138433/range2138433/1
component: BBa_K575013

https://synbiohub.org/public/igem/BBa_K575036/annotation2138433/range2138433/1
type: Range
persistent identity: range2138433
display id: range2138433
version: 1
direction: direction/reverse
top level: BBa_K575036
start: 1
end: 799
orientation: inline

BBa_K575006
type: SequenceAnnotation
persistent identity: annotation2138439
display id: annotation2138439
version: 1
top level: BBa_K575036
location: https://synbiohub.org/public/igem/BBa_K575036/annotation2138439/range2138439/1
component: BBa_K575006

https://synbiohub.org/public/igem/BBa_K575036/annotation2138439/range2138439/1
type: Range
persistent identity: range2138439
display id: range2138439
version: 1
direction: direction/reverse
top level: BBa_K575036
start: 808
end: 1600
orientation: inline

BBa_K575013
type: ComponentDefinition
persistent identity: BBa K575013
display id: BBa_K575013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP
creator: Kristin Palarz
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR/PAI2 inducable promoter with RBS (B0034) and GFP.
mutable notes: Need to ensure no leaky transcription.
mutable provenance: From BioBrick Parts Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0040
component: BBa_R0071
sequence annotation: BBa_B0034
sequence annotation: BBa_R0071
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K575013_sequence/1

BBa_B0034
type: Component
persistent identity: component2124869
display id: component2124869
version: 1
top level: BBa_K575013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2124871
display id: component2124871
version: 1
top level: BBa_K575013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0071
type: Component
persistent identity: component2124867
display id: component2124867
version: 1
top level: BBa_K575013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2124869
display id: annotation2124869
version: 1
top level: BBa_K575013
location: https://synbiohub.org/public/igem/BBa_K575013/annotation2124869/range2124869/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K575013/annotation2124869/range2124869/1
type: Range
persistent identity: range2124869
display id: range2124869
version: 1
direction: direction/reverse
top level: BBa_K575013
start: 62
end: 73
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2124867
display id: annotation2124867
version: 1
top level: BBa_K575013
location: https://synbiohub.org/public/igem/BBa_K575013/annotation2124867/range2124867/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K575013/annotation2124867/range2124867/1
type: Range
persistent identity: range2124867
display id: range2124867
version: 1
direction: direction/reverse
top level: BBa_K575013
start: 1
end: 53
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2124871
display id: annotation2124871
version: 1
top level: BBa_K575013
location: https://synbiohub.org/public/igem/BBa_K575013/annotation2124871/range2124871/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K575013/annotation2124871/range2124871/1
type: Range
persistent identity: range2124871
display id: range2124871
version: 1
direction: direction/reverse
top level: BBa_K575013
start: 80
end: 799
orientation: inline

BBa_K817021
type: ComponentDefinition
persistent identity: BBa K817021
display id: BBa_K817021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-SP1-Penetratin-Flag
creator: Jui-Tsen Yu
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1075_
m user id: 0
owner id: 13572
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS-SP1-xx
mutable notes: RBS-SP1-xx
mutable provenance: RBS-SP1-xx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0030
component: BBa_K817027
sequence annotation: BBa_B0030
sequence annotation: BBa_K817027
sequence: https://synbiohub.org/public/igem/BBa_K817021_sequence/1

BBa_B0030
type: Component
persistent identity: component2201518
display id: component2201518
version: 1
top level: BBa_K817021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K817027
type: Component
persistent identity: component2201523
display id: component2201523
version: 1
top level: BBa_K817021
access: public
definition: https://synbiohub.org/public/igem/BBa_K817027/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2201518
display id: annotation2201518
version: 1
top level: BBa_K817021
location: https://synbiohub.org/public/igem/BBa_K817021/annotation2201518/range2201518/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K817021/annotation2201518/range2201518/1
type: Range
persistent identity: range2201518
display id: range2201518
version: 1
direction: direction/forward
top level: BBa_K817021
start: 1
end: 15
orientation: inline

BBa_K817027
type: SequenceAnnotation
persistent identity: annotation2201523
display id: annotation2201523
version: 1
top level: BBa_K817021
location: https://synbiohub.org/public/igem/BBa_K817021/annotation2201523/range2201523/1
component: BBa_K817027

https://synbiohub.org/public/igem/BBa_K817021/annotation2201523/range2201523/1
type: Range
persistent identity: range2201523
display id: range2201523
version: 1
direction: direction/reverse
top level: BBa_K817021
start: 22
end: 162
orientation: inline

BBa_K808025
type: ComponentDefinition
persistent identity: BBa K808025
display id: BBa_K808025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FsC: Cutinase PET cleaving enzyme
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A cutinase is a cuticula degrading hydrolase from the fungus Fusarium solani pisi. It shows activity towards PET.
mutable notes: Surface Expression is very hard to do. FsC shows lipolytic activity and attacks the outer membrane of gramm negativ bacteria. This activity leads to a certain toxicity for expression hosts. In addition FsC needs to be expressed in the periplasm due to folding issues.
mutable provenance: Cloned from vector pEST100 from Becker et all 2005: (Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808025_sequence/1

BBa_K590053
type: ComponentDefinition
persistent identity: BBa K590053
display id: BBa_K590053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RrD_PSB1C3-High constitutive
creator: Lei Zheng, Ben Mo, Elaine Lai, Juhye An
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Plasmid that consists of Acyl-ACP Reductase, rbs, normal Synechococcus elongatus PCC7942 Aldehyde Decarbonylase in PSB1C3 high consititutive vector for alkane production
mutable notes: TBD
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K314100
component: BBa_K590032
component: BBa_K590031
component: BBa_B0034
sequence annotation: BBa_K590031
sequence annotation: BBa_B0034
sequence annotation: BBa_K314100
sequence annotation: BBa_K590032
sequence: https://synbiohub.org/public/igem/BBa_K590053_sequence/1

BBa_K314100
type: Component
persistent identity: component2129327
display id: component2129327
version: 1
top level: BBa_K590053
access: public
definition: BBa_K314100

BBa_K590032
type: Component
persistent identity: component2129328
display id: component2129328
version: 1
top level: BBa_K590053
access: public
definition: https://synbiohub.org/public/igem/BBa_K590032/1

BBa_K590031
type: Component
persistent identity: component2129331
display id: component2129331
version: 1
top level: BBa_K590053
access: public
definition: https://synbiohub.org/public/igem/BBa_K590031/1

BBa_B0034
type: Component
persistent identity: component2129330
display id: component2129330
version: 1
top level: BBa_K590053
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K590031
type: SequenceAnnotation
persistent identity: annotation2129331
display id: annotation2129331
version: 1
top level: BBa_K590053
location: https://synbiohub.org/public/igem/BBa_K590053/annotation2129331/range2129331/1
component: BBa_K590031

https://synbiohub.org/public/igem/BBa_K590053/annotation2129331/range2129331/1
type: Range
persistent identity: range2129331
display id: range2129331
version: 1
direction: direction/reverse
top level: BBa_K590053
start: 1637
end: 2392
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2129330
display id: annotation2129330
version: 1
top level: BBa_K590053
location: https://synbiohub.org/public/igem/BBa_K590053/annotation2129330/range2129330/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K590053/annotation2129330/range2129330/1
type: Range
persistent identity: range2129330
display id: range2129330
version: 1
direction: direction/reverse
top level: BBa_K590053
start: 1619
end: 1630
orientation: inline

BBa_K314100
type: SequenceAnnotation
persistent identity: annotation2129327
display id: annotation2129327
version: 1
top level: BBa_K590053
location: https://synbiohub.org/public/igem/BBa_K590053/annotation2129327/range2129327/1
component: BBa_K314100

https://synbiohub.org/public/igem/BBa_K590053/annotation2129327/range2129327/1
type: Range
persistent identity: range2129327
display id: range2129327
version: 1
direction: direction/reverse
top level: BBa_K590053
start: 1
end: 518
orientation: inline

BBa_K590032
type: SequenceAnnotation
persistent identity: annotation2129328
display id: annotation2129328
version: 1
top level: BBa_K590053
location: https://synbiohub.org/public/igem/BBa_K590053/annotation2129328/range2129328/1
component: BBa_K590032

https://synbiohub.org/public/igem/BBa_K590053/annotation2129328/range2129328/1
type: Range
persistent identity: range2129328
display id: range2129328
version: 1
direction: direction/reverse
top level: BBa_K590053
start: 525
end: 1610
orientation: inline

BBa_K738002
type: ComponentDefinition
persistent identity: BBa K738002
display id: BBa_K738002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophyline riboswitch regulated RNA Scaffold (clover vision 2)
creator: Huachun Liu
created: 2012-08-08T11:00:00Z
modified: 2015-05-08T01:13:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _987_
m user id: 0
owner id: 11753
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins. A riboswitch of theophyline is added in the stucture of D0 to regulate its function.
mutable notes: ttaatacgactcactatagggggggcccgggaaccagcgacgggcccggcagcaccgaggaggactcccacagtcactggggagtcctcgaatacgagctgggcacagaagatatggcttcgtgcccaggaagtgttcgcacttctctcgtattcgccccctagcataaccccttggggcctctaaacgggtcttgaggggtttttttg
mutable provenance: The secquence was commercially synthesized by??????
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T7 promoter
sequence annotation: PP7 aptamer
sequence annotation: MS2 aptamer
sequence annotation: T7 terminator
sequence annotation: Theophylline aptamer
sequence: https://synbiohub.org/public/igem/BBa_K738002_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2195918
display id: annotation2195918
version: 1
top level: BBa_K738002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K738002/annotation2195918/range2195918/1

https://synbiohub.org/public/igem/BBa_K738002/annotation2195918/range2195918/1
type: Range
persistent identity: range2195918
display id: range2195918
version: 1
direction: direction/forward
top level: BBa_K738002
start: 1
end: 21
orientation: inline

PP7 aptamer
type: SequenceAnnotation
persistent identity: annotation2195921
display id: annotation2195921
version: 1
top level: BBa_K738002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K738002/annotation2195921/range2195921/1

https://synbiohub.org/public/igem/BBa_K738002/annotation2195921/range2195921/1
type: Range
persistent identity: range2195921
display id: range2195921
version: 1
direction: direction/forward
top level: BBa_K738002
start: 109
end: 134
orientation: inline

MS2 aptamer
type: SequenceAnnotation
persistent identity: annotation2195919
display id: annotation2195919
version: 1
top level: BBa_K738002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K738002/annotation2195919/range2195919/1

https://synbiohub.org/public/igem/BBa_K738002/annotation2195919/range2195919/1
type: Range
persistent identity: range2195919
display id: range2195919
version: 1
direction: direction/forward
top level: BBa_K738002
start: 76
end: 89
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2195922
display id: annotation2195922
version: 1
top level: BBa_K738002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K738002/annotation2195922/range2195922/1

https://synbiohub.org/public/igem/BBa_K738002/annotation2195922/range2195922/1
type: Range
persistent identity: range2195922
display id: range2195922
version: 1
direction: direction/forward
top level: BBa_K738002
start: 165
end: 217
orientation: inline

Theophylline aptamer
type: SequenceAnnotation
persistent identity: annotation2211644
display id: annotation2211644
version: 1
top level: BBa_K738002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K738002/annotation2211644/range2211644/1

https://synbiohub.org/public/igem/BBa_K738002/annotation2211644/range2211644/1
type: Range
persistent identity: range2211644
display id: range2211644
version: 1
direction: direction/forward
top level: BBa_K738002
start: 31
end: 66
orientation: inline

BBa_K575020
type: ComponentDefinition
persistent identity: BBa K575020
display id: BBa_K575020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Genomic LasR/PAI1 Inducible Promoter (Long)
creator: Michael Vincent Sherer
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8753
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the PAI-1/LasR complex, part of the Pseudomonas quorum sensing hierarchy.
mutable notes: The part is unusually long for a promoter because of the primers we used to extract it. We do not know exactly where the regulatory region is.
mutable provenance: It is the upstream regulatory region of [gene].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K575020_sequence/1

BBa_K530005
type: ComponentDefinition
persistent identity: BBa K530005
display id: BBa_K530005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BAP2 Yeast Promoter
creator: Daniel Wolozny
created: 2011-08-03T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _696_
m user id: 0
owner id: 8447
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BAP2 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
mutable notes: Promoter was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the promoter.
mutable provenance: http://www.yeastgenome.org/cgi-bin/locus.fpl
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BAP2p
sequence: https://synbiohub.org/public/igem/BBa_K530005_sequence/1

BAP2p
type: SequenceAnnotation
persistent identity: annotation2138571
display id: annotation2138571
version: 1
top level: BBa_K530005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K530005/annotation2138571/range2138571/1

https://synbiohub.org/public/igem/BBa_K530005/annotation2138571/range2138571/1
type: Range
persistent identity: range2138571
display id: range2138571
version: 1
direction: direction/forward
top level: BBa_K530005
start: 1
end: 800
orientation: inline

BBa_K530000
type: ComponentDefinition
persistent identity: BBa K530000
display id: BBa_K530000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CRTYB
creator: Daniel Wolozny
created: 2011-07-31T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _696_
m user id: 0
owner id: 8447
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: First enzyme in the pathway required for B-Carotene Synthesis. This enzyme is also known as Geranylgeranyl diphosphate synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Fernesyl Diphosphate to Geranylgeranyl diphosphate
mutable notes: Part was PCR amplified from the genomic DNA created from the experiment and paper: High-Level Production of Beta-Carotene in Saccharomyces cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces dendrorhous (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932764/).
mutable provenance: Found in the genomic sequence of Xanthophyllomyces Dendrorhous. http://www.ncbi.nlm.nih.gov/nuccore/AY177204.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CrtYB
sequence: https://synbiohub.org/public/igem/BBa_K530000_sequence/1

CrtYB
type: SequenceAnnotation
persistent identity: annotation2139113
display id: annotation2139113
version: 1
top level: BBa_K530000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K530000/annotation2139113/range2139113/1

https://synbiohub.org/public/igem/BBa_K530000/annotation2139113/range2139113/1
type: Range
persistent identity: range2139113
display id: range2139113
version: 1
direction: direction/forward
top level: BBa_K530000
start: 1
end: 2022
orientation: inline

sinI
type: ComponentDefinition
persistent identity: BBa K594001
display id: BBa_K594001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a luxI homologous AHL synthetase from Sinorhizobium meliloti 1021
creator: Yu Xiaoyan
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _765_
m user id: 0
owner id: 8221
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The Sinorhizobium meliloti strain Rm1021 harbors at least two quorum-sensing systems; one of them is sinR/sinI system, The sinI is responsible for the production of several novel AHLs, including C12-HSL, 3-Oxo-C14-HSL, C16:1-HSL, 3-Oxo-C16:1-HSL, C18-HSL, some of theses are the longest AHLs identified so far. The sin system correlates with a delay in the appearance of nitrogen-fixing nodules, as well as with an overall decrease in the number of pink nodules, suggesting a role for quorum sensing in establishing a successful symbiosis with Medicago sativa. As a autoinducer synthatase, the use of sinI is the same with luxI, namely to produce AHLS.
mutable notes: The Sinorhizobium meliloti 1021 is a mode organism in Rhizobia. so We got the S. meliloti 1021 from Nanjing Agriculture University easily, and then cloned the sinI gene from S. meliloti 1021 with primers designed by the part design tool in the website ginkgobioworks.com.
mutable provenance: SinI is from the Sinorhizobium meliloti 1021. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sinI
sequence: https://synbiohub.org/public/igem/BBa_K594001_sequence/1

sinI
type: SequenceAnnotation
persistent identity: annotation2147997
display id: annotation2147997
version: 1
top level: sinI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K594001/annotation2147997/range2147997/1

https://synbiohub.org/public/igem/BBa_K594001/annotation2147997/range2147997/1
type: Range
persistent identity: range2147997
display id: range2147997
version: 1
direction: direction/forward
top level: sinI
start: 1
end: 645
orientation: inline

BBa_K611018
type: ComponentDefinition
persistent identity: BBa K611018
display id: BBa_K611018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutant Regulatory protein/promoter characterization plasmid
creator: Timothy Fenton
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
group u list: _783_
m user id: 0
owner id: 4722
owning group id: 415
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part can be used to characterize promoter/repressor and promoter/activator pairs by swapping in the desired parts on either end. To characterize promoter mutants, ligate the corresponding repressor or activator on the 3' end by cutting the plasmid with speI and pstI and the regulatory protein with XbaI and pstI. Then put the desired mutant promoter on the 5' end. Repressor/activator mutants can also be characterized by simply putting a wildtype promoter on the 5' end and adding the mutant repressor or activator on the 3' end. Expression is reported via GFP fluorescence. The amount of repressor/activator can be controlled using the arabinose inducible pBAD promoter. Transcriptional termination takes place within the psb1C3 plasmid which means a terminator does not need to be added after the inserted coding sequence is added.
mutable notes: When a protein coding sequence is added, there is no need to add a terminator since termination happens in the psb1C3 plasmid.
mutable provenance: This part comes entirely from parts already available in the Parts Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0015
component: BBa_I13453
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_I13453
sequence annotation: BBa_B0015
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K611018_sequence/1

BBa_B0034
type: Component
persistent identity: component2137231
display id: component2137231
version: 1
top level: BBa_K611018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2137228
display id: component2137228
version: 1
top level: BBa_K611018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I13453
type: Component
persistent identity: component2137229
display id: component2137229
version: 1
top level: BBa_K611018
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_E0040
type: Component
persistent identity: component2137221
display id: component2137221
version: 1
top level: BBa_K611018
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component2137219
display id: component2137219
version: 1
top level: BBa_K611018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2137219
display id: annotation2137219
version: 1
top level: BBa_K611018
location: https://synbiohub.org/public/igem/BBa_K611018/annotation2137219/range2137219/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611018/annotation2137219/range2137219/1
type: Range
persistent identity: range2137219
display id: range2137219
version: 1
direction: direction/reverse
top level: BBa_K611018
start: 1
end: 12
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2137231
display id: annotation2137231
version: 1
top level: BBa_K611018
location: https://synbiohub.org/public/igem/BBa_K611018/annotation2137231/range2137231/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611018/annotation2137231/range2137231/1
type: Range
persistent identity: range2137231
display id: range2137231
version: 1
direction: direction/reverse
top level: BBa_K611018
start: 1022
end: 1033
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2137229
display id: annotation2137229
version: 1
top level: BBa_K611018
location: https://synbiohub.org/public/igem/BBa_K611018/annotation2137229/range2137229/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K611018/annotation2137229/range2137229/1
type: Range
persistent identity: range2137229
display id: range2137229
version: 1
direction: direction/reverse
top level: BBa_K611018
start: 884
end: 1013
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2137228
display id: annotation2137228
version: 1
top level: BBa_K611018
location: https://synbiohub.org/public/igem/BBa_K611018/annotation2137228/range2137228/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K611018/annotation2137228/range2137228/1
type: Range
persistent identity: range2137228
display id: range2137228
version: 1
direction: direction/reverse
top level: BBa_K611018
start: 747
end: 875
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2137221
display id: annotation2137221
version: 1
top level: BBa_K611018
location: https://synbiohub.org/public/igem/BBa_K611018/annotation2137221/range2137221/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K611018/annotation2137221/range2137221/1
type: Range
persistent identity: range2137221
display id: range2137221
version: 1
direction: direction/reverse
top level: BBa_K611018
start: 19
end: 738
orientation: inline

BBa_K577006
type: ComponentDefinition
persistent identity: BBa K577006
display id: BBa_K577006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP Coding Device
creator: Vanessa Yanez
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: true
group u list: _748_
m user id: 0
owner id: 9101
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is device that includes an inducible promoter, but requires the insertion of AraC to be able to induce the promoter with arabinose.
mutable notes: The RBS, YFP, and double terminator come from BBa_E0430.
mutable provenance: Spring 2011 Kit Plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E0030
component: BBa_B0015
component: BBa_I13453
sequence annotation: BBa_B0015
sequence annotation: BBa_E0030
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K577006_sequence/1

BBa_B0034
type: Component
persistent identity: component2126972
display id: component2126972
version: 1
top level: BBa_K577006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component2126973
display id: component2126973
version: 1
top level: BBa_K577006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0015
type: Component
persistent identity: component2126980
display id: component2126980
version: 1
top level: BBa_K577006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I13453
type: Component
persistent identity: component2126970
display id: component2126970
version: 1
top level: BBa_K577006
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2126980
display id: annotation2126980
version: 1
top level: BBa_K577006
location: https://synbiohub.org/public/igem/BBa_K577006/annotation2126980/range2126980/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K577006/annotation2126980/range2126980/1
type: Range
persistent identity: range2126980
display id: range2126980
version: 1
direction: direction/reverse
top level: BBa_K577006
start: 888
end: 1016
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2126973
display id: annotation2126973
version: 1
top level: BBa_K577006
location: https://synbiohub.org/public/igem/BBa_K577006/annotation2126973/range2126973/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K577006/annotation2126973/range2126973/1
type: Range
persistent identity: range2126973
display id: range2126973
version: 1
direction: direction/reverse
top level: BBa_K577006
start: 157
end: 879
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2126970
display id: annotation2126970
version: 1
top level: BBa_K577006
location: https://synbiohub.org/public/igem/BBa_K577006/annotation2126970/range2126970/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K577006/annotation2126970/range2126970/1
type: Range
persistent identity: range2126970
display id: range2126970
version: 1
direction: direction/reverse
top level: BBa_K577006
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126972
display id: annotation2126972
version: 1
top level: BBa_K577006
location: https://synbiohub.org/public/igem/BBa_K577006/annotation2126972/range2126972/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K577006/annotation2126972/range2126972/1
type: Range
persistent identity: range2126972
display id: range2126972
version: 1
direction: direction/reverse
top level: BBa_K577006
start: 139
end: 150
orientation: inline

BBa_K575012
type: ComponentDefinition
persistent identity: BBa K575012
display id: BBa_K575012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP
creator: Kristin Palarz
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR/PAI2 inducable promoter with RBS (B0030) and GFP.
mutable notes: Need to ensure no leaky transcription.
mutable provenance: From BioBrick Parts Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_R0071
component: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_R0071
sequence: https://synbiohub.org/public/igem/BBa_K575012_sequence/1

BBa_E0040
type: Component
persistent identity: component2124862
display id: component2124862
version: 1
top level: BBa_K575012
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0071
type: Component
persistent identity: component2124857
display id: component2124857
version: 1
top level: BBa_K575012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0030
type: Component
persistent identity: component2124859
display id: component2124859
version: 1
top level: BBa_K575012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2124862
display id: annotation2124862
version: 1
top level: BBa_K575012
location: https://synbiohub.org/public/igem/BBa_K575012/annotation2124862/range2124862/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K575012/annotation2124862/range2124862/1
type: Range
persistent identity: range2124862
display id: range2124862
version: 1
direction: direction/reverse
top level: BBa_K575012
start: 83
end: 802
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2124859
display id: annotation2124859
version: 1
top level: BBa_K575012
location: https://synbiohub.org/public/igem/BBa_K575012/annotation2124859/range2124859/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K575012/annotation2124859/range2124859/1
type: Range
persistent identity: range2124859
display id: range2124859
version: 1
direction: direction/forward
top level: BBa_K575012
start: 62
end: 76
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2124857
display id: annotation2124857
version: 1
top level: BBa_K575012
location: https://synbiohub.org/public/igem/BBa_K575012/annotation2124857/range2124857/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K575012/annotation2124857/range2124857/1
type: Range
persistent identity: range2124857
display id: range2124857
version: 1
direction: direction/reverse
top level: BBa_K575012
start: 1
end: 53
orientation: inline

BBa_K769001
type: ComponentDefinition
persistent identity: BBa K769001
display id: BBa_K769001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PompC-RBS-GFP-Double terminator
creator: Genki Sakai
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1021_
m user id: 0
owner id: 14289
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PompR-RBS-GFP-Double terminator
mutable notes: PompR-RBS-GFP-Double terminator
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: GFP
sequence annotation: BBa_R0082
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K769001_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2199931
display id: annotation2199931
version: 1
top level: BBa_K769001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K769001/annotation2199931/range2199931/1

https://synbiohub.org/public/igem/BBa_K769001/annotation2199931/range2199931/1
type: Range
persistent identity: range2199931
display id: range2199931
version: 1
direction: direction/forward
top level: BBa_K769001
start: 137
end: 853
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2199928
display id: annotation2199928
version: 1
top level: BBa_K769001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K769001/annotation2199928/range2199928/1

https://synbiohub.org/public/igem/BBa_K769001/annotation2199928/range2199928/1
type: Range
persistent identity: range2199928
display id: range2199928
version: 1
direction: direction/forward
top level: BBa_K769001
start: 1
end: 108
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2199932
display id: annotation2199932
version: 1
top level: BBa_K769001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K769001/annotation2199932/range2199932/1

https://synbiohub.org/public/igem/BBa_K769001/annotation2199932/range2199932/1
type: Range
persistent identity: range2199932
display id: range2199932
version: 1
direction: direction/forward
top level: BBa_K769001
start: 862
end: 990
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2199930
display id: annotation2199930
version: 1
top level: BBa_K769001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K769001/annotation2199930/range2199930/1

https://synbiohub.org/public/igem/BBa_K769001/annotation2199930/range2199930/1
type: Range
persistent identity: range2199930
display id: range2199930
version: 1
direction: direction/forward
top level: BBa_K769001
start: 117
end: 128
orientation: inline

BBa_K539623
type: ComponentDefinition
persistent identity: BBa K539623
display id: BBa_K539623
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a RBS (B0034)+ilvC
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_K539711
sequence annotation: BBa_B0034
sequence annotation: BBa_K539711
sequence: https://synbiohub.org/public/igem/BBa_K539623_sequence/1

BBa_B0034
type: Component
persistent identity: component2143926
display id: component2143926
version: 1
top level: BBa_K539623
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K539711
type: Component
persistent identity: component2143928
display id: component2143928
version: 1
top level: BBa_K539623
access: public
definition: https://synbiohub.org/public/igem/BBa_K539711/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2143926
display id: annotation2143926
version: 1
top level: BBa_K539623
location: https://synbiohub.org/public/igem/BBa_K539623/annotation2143926/range2143926/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K539623/annotation2143926/range2143926/1
type: Range
persistent identity: range2143926
display id: range2143926
version: 1
direction: direction/reverse
top level: BBa_K539623
start: 1
end: 12
orientation: inline

BBa_K539711
type: SequenceAnnotation
persistent identity: annotation2143928
display id: annotation2143928
version: 1
top level: BBa_K539623
location: https://synbiohub.org/public/igem/BBa_K539623/annotation2143928/range2143928/1
component: BBa_K539711

https://synbiohub.org/public/igem/BBa_K539623/annotation2143928/range2143928/1
type: Range
persistent identity: range2143928
display id: range2143928
version: 1
direction: direction/reverse
top level: BBa_K539623
start: 19
end: 1494
orientation: inline

SYFP2
type: ComponentDefinition
persistent identity: BBa K864100
display id: BBa_K864100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Super Yellow Fluorescent Protein 2 (SYFP2)
creator: Erik Gullberg
created: 2012-09-23T11:00:00Z
modified: 2016-01-21T02:29:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 4206
owner id: 10137
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K.
mutable notes: The sequence has been codon optimized for expression in E coli by DNA 2.0.
mutable provenance: Amino acid sequence taken from the article by Kremers et al 2006.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SYFP2
sequence: https://synbiohub.org/public/igem/BBa_K864100_sequence/1

SYFP2
type: SequenceAnnotation
persistent identity: annotation2196221
display id: annotation2196221
version: 1
top level: SYFP2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864100/annotation2196221/range2196221/1

https://synbiohub.org/public/igem/BBa_K864100/annotation2196221/range2196221/1
type: Range
persistent identity: range2196221
display id: range2196221
version: 1
direction: direction/forward
top level: SYFP2
start: 1
end: 717
orientation: inline

BBa_K598000
type: ComponentDefinition
persistent identity: BBa K598000
display id: BBa_K598000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015
creator: Yangyang ZHAO, Yan GONG, Zhenrun ZHANG, Qingyang XIAO
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 4411
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is TPP up-regulated hammerhead ribozyme, consisting of 131 nucleotides.
mutable notes: This part contains a native ribosomal binding site
mutable provenance: This part was an engineered RNA controller composed of a TPP aptamer from B.subtilis and a hammerhead motif.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0012
sequence annotation: eGFP
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: stop
sequence annotation: TPP Ribozyme 2.5
sequence annotation: BBa_E0040
sequence annotation: T7 TE
sequence annotation: native RBS
sequence annotation: BBa_B0015
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_K598000_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152867
display id: annotation2152867
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152867/range2152867/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152867/range2152867/1
type: Range
persistent identity: range2152867
display id: range2152867
version: 1
direction: direction/forward
top level: BBa_K598000
start: 967
end: 1007
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152835
display id: annotation2152835
version: 1
top level: BBa_K598000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152835/range2152835/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152835/range2152835/1
type: Range
persistent identity: range2152835
display id: range2152835
version: 1
direction: direction/forward
top level: BBa_K598000
start: 151
end: 870
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152836
display id: annotation2152836
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152836/range2152836/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152836/range2152836/1
type: Range
persistent identity: range2152836
display id: range2152836
version: 1
direction: direction/forward
top level: BBa_K598000
start: 879
end: 958
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152857
display id: annotation2152857
version: 1
top level: BBa_K598000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152857/range2152857/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152857/range2152857/1
type: Range
persistent identity: range2152857
display id: range2152857
version: 1
direction: direction/forward
top level: BBa_K598000
start: 890
end: 933
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2152872
display id: annotation2152872
version: 1
top level: BBa_K598000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152872/range2152872/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152872/range2152872/1
type: Range
persistent identity: range2152872
display id: range2152872
version: 1
direction: direction/forward
top level: BBa_K598000
start: 1000
end: 1000
orientation: inline

TPP Ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2152875
display id: annotation2152875
version: 1
top level: BBa_K598000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152875/range2152875/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152875/range2152875/1
type: Range
persistent identity: range2152875
display id: range2152875
version: 1
direction: direction/forward
top level: BBa_K598000
start: 1
end: 150
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152880
display id: annotation2152880
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152880/range2152880/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152880/range2152880/1
type: Range
persistent identity: range2152880
display id: range2152880
version: 1
direction: direction/forward
top level: BBa_K598000
start: 151
end: 870
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152868
display id: annotation2152868
version: 1
top level: BBa_K598000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152868/range2152868/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152868/range2152868/1
type: Range
persistent identity: range2152868
display id: range2152868
version: 1
direction: direction/forward
top level: BBa_K598000
start: 974
end: 993
orientation: inline

native RBS
type: SequenceAnnotation
persistent identity: annotation2131876
display id: annotation2131876
version: 1
top level: BBa_K598000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2131876/range2131876/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2131876/range2131876/1
type: Range
persistent identity: range2131876
display id: range2131876
version: 1
direction: direction/forward
top level: BBa_K598000
start: 138
end: 145
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153033
display id: annotation2153033
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2153033/range2153033/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2153033/range2153033/1
type: Range
persistent identity: range2153033
display id: range2153033
version: 1
direction: direction/forward
top level: BBa_K598000
start: 879
end: 1007
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2152870
display id: annotation2152870
version: 1
top level: BBa_K598000
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152870/range2152870/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152870/range2152870/1
type: Range
persistent identity: range2152870
display id: range2152870
version: 1
direction: direction/forward
top level: BBa_K598000
start: 994
end: 1007
orientation: inline

BBa_K574007
type: ComponentDefinition
persistent identity: BBa K574007
display id: BBa_K574007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR + TT
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lasR + T.
mutable notes: Empty.
mutable provenance: Existing Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0079
sequence annotation: BBa_B0015
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_K574007_sequence/1

BBa_B0015
type: Component
persistent identity: component2248081
display id: component2248081
version: 1
top level: BBa_K574007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0079
type: Component
persistent identity: component2248074
display id: component2248074
version: 1
top level: BBa_K574007
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2248081
display id: annotation2248081
version: 1
top level: BBa_K574007
location: https://synbiohub.org/public/igem/BBa_K574007/annotation2248081/range2248081/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K574007/annotation2248081/range2248081/1
type: Range
persistent identity: range2248081
display id: range2248081
version: 1
direction: direction/reverse
top level: BBa_K574007
start: 790
end: 918
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2248074
display id: annotation2248074
version: 1
top level: BBa_K574007
location: https://synbiohub.org/public/igem/BBa_K574007/annotation2248074/range2248074/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K574007/annotation2248074/range2248074/1
type: Range
persistent identity: range2248074
display id: range2248074
version: 1
direction: direction/reverse
top level: BBa_K574007
start: 1
end: 781
orientation: inline

BBa_K823004
type: ComponentDefinition
persistent identity: BBa K823004
display id: BBa_K823004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23100
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:43:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23100 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23100
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823004_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation2182506
display id: annotation2182506
version: 1
top level: BBa_K823004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1
type: Range
persistent identity: range2182506
display id: range2182506
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182507
display id: annotation2182507
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1
type: Range
persistent identity: range2182507
display id: range2182507
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182508
display id: annotation2182508
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1
type: Range
persistent identity: range2182508
display id: range2182508
version: 1
direction: direction/forward
top level: BBa_K823004
start: 24
end: 29
orientation: inline

BBa_S05050
type: ComponentDefinition
persistent identity: BBa S05050
display id: BBa_S05050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:K748002
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:14:49Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K748002
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K748002
sequence: https://synbiohub.org/public/igem/BBa_S05050_sequence/1

BBa_K748002
type: Component
persistent identity: component2183419
display id: component2183419
version: 1
top level: BBa_S05050
access: public
definition: BBa_K748002

BBa_B0034
type: Component
persistent identity: component2183418
display id: component2183418
version: 1
top level: BBa_S05050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2183418
display id: annotation2183418
version: 1
top level: BBa_S05050
location: https://synbiohub.org/public/igem/BBa_S05050/annotation2183418/range2183418/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05050/annotation2183418/range2183418/1
type: Range
persistent identity: range2183418
display id: range2183418
version: 1
direction: direction/reverse
top level: BBa_S05050
start: 1
end: 12
orientation: inline

BBa_K748002
type: SequenceAnnotation
persistent identity: annotation2183419
display id: annotation2183419
version: 1
top level: BBa_S05050
location: https://synbiohub.org/public/igem/BBa_S05050/annotation2183419/range2183419/1
component: BBa_K748002

https://synbiohub.org/public/igem/BBa_S05050/annotation2183419/range2183419/1
type: Range
persistent identity: range2183419
display id: range2183419
version: 1
direction: direction/reverse
top level: BBa_S05050
start: 19
end: 759
orientation: inline

BBa_K542003
type: ComponentDefinition
persistent identity: BBa K542003
display id: BBa_K542003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD Regulated TetR
creator: Anthony Vuong
created: 2011-09-22T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Made by assembling [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] with [http://partsregistry.org/Part:BBa_P0440 BBa_P0440]. Production of TetR is regulated by the pBAD promoter([http://partsregistry.org/Part:BBa_I13453 BBa_I13453]). TetR is an inhibitor of the constitutively "on" promoter pTet--[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 BBa_R0040]). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. Arabinose -> pBAD "on" -> TetR -> pTet "off" Intermediate part to assemble the "Co-localization Construct".
mutable notes: Intermediate part to assemble the "Co-localization Construct".
mutable provenance: [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] and [http://partsregistry.org/Part:BBa_P0440 BBa_P0440] were both obtained from iGEM distribution plates.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_P0440
component: BBa_I13453
sequence annotation: BBa_P0440
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K542003_sequence/1

BBa_P0440
type: Component
persistent identity: component2138601
display id: component2138601
version: 1
top level: BBa_K542003
access: public
definition: https://synbiohub.org/public/igem/BBa_P0440/1

BBa_I13453
type: Component
persistent identity: component2138588
display id: component2138588
version: 1
top level: BBa_K542003
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_P0440
type: SequenceAnnotation
persistent identity: annotation2138601
display id: annotation2138601
version: 1
top level: BBa_K542003
location: https://synbiohub.org/public/igem/BBa_K542003/annotation2138601/range2138601/1
component: BBa_P0440

https://synbiohub.org/public/igem/BBa_K542003/annotation2138601/range2138601/1
type: Range
persistent identity: range2138601
display id: range2138601
version: 1
direction: direction/reverse
top level: BBa_K542003
start: 139
end: 978
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2138588
display id: annotation2138588
version: 1
top level: BBa_K542003
location: https://synbiohub.org/public/igem/BBa_K542003/annotation2138588/range2138588/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K542003/annotation2138588/range2138588/1
type: Range
persistent identity: range2138588
display id: range2138588
version: 1
direction: direction/reverse
top level: BBa_K542003
start: 1
end: 130
orientation: inline

BBa_K929302
type: ComponentDefinition
persistent identity: BBa K929302
display id: BBa_K929302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AID in Potsdam Standard
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14525
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes.
mutable notes: AID was amplified with primers which contains thiophosphates at 5' end and insert in Potsdam Standard Backbone using PLIcing method.
mutable provenance: BBa_K103001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AID
sequence: https://synbiohub.org/public/igem/BBa_K929302_sequence/1

AID
type: SequenceAnnotation
persistent identity: annotation2195668
display id: annotation2195668
version: 1
top level: BBa_K929302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K929302/annotation2195668/range2195668/1

https://synbiohub.org/public/igem/BBa_K929302/annotation2195668/range2195668/1
type: Range
persistent identity: range2195668
display id: range2195668
version: 1
direction: direction/forward
top level: BBa_K929302
start: 9
end: 606
orientation: inline

BBa_K575027
type: ComponentDefinition
persistent identity: BBa K575027
display id: BBa_K575027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0034) + LasR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of LasR combined with receptor
mutable notes: put the constitutive promoter after the production of LasR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575005
component: BBa_K575010
sequence annotation: BBa_K575005
sequence annotation: BBa_K575010
sequence: https://synbiohub.org/public/igem/BBa_K575027_sequence/1

BBa_K575005
type: Component
persistent identity: component2264144
display id: component2264144
version: 1
top level: BBa_K575027
access: public
definition: https://synbiohub.org/public/igem/BBa_K575005/1

BBa_K575010
type: Component
persistent identity: component2264139
display id: component2264139
version: 1
top level: BBa_K575027
access: public
definition: BBa_K575010

BBa_K575005
type: SequenceAnnotation
persistent identity: annotation2264144
display id: annotation2264144
version: 1
top level: BBa_K575027
location: https://synbiohub.org/public/igem/BBa_K575027/annotation2264144/range2264144/1
component: BBa_K575005

https://synbiohub.org/public/igem/BBa_K575027/annotation2264144/range2264144/1
type: Range
persistent identity: range2264144
display id: range2264144
version: 1
direction: direction/reverse
top level: BBa_K575027
start: 901
end: 1684
orientation: inline

BBa_K575010
type: SequenceAnnotation
persistent identity: annotation2264139
display id: annotation2264139
version: 1
top level: BBa_K575027
location: https://synbiohub.org/public/igem/BBa_K575027/annotation2264139/range2264139/1
component: BBa_K575010

https://synbiohub.org/public/igem/BBa_K575027/annotation2264139/range2264139/1
type: Range
persistent identity: range2264139
display id: range2264139
version: 1
direction: direction/reverse
top level: BBa_K575027
start: 1
end: 892
orientation: inline

BBa_K510022
type: ComponentDefinition
persistent identity: BBa K510022
display id: BBa_K510022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: attTn7
creator: David Caballero
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: TnsABC+D transposase machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. This insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the miniTn7 BioBrick toolkit in any organism as well as in plasmids. All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.
mutable notes: The attTn7 site was flanked by prefix and suffix for cloning purposes.
mutable provenance: The attTn7 was obtained from the purified genome of E. coli MC4100 by PCR (using a high accuracy Pfu polymerase) with these primers: gaattcgcggccgcttctagagTGCAGCTGCTGGCTTACCATG and ctgcagcggccgctactagtaACATGGAGTTGGCAGGATGTTTG.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K510022_sequence/1

BBa_K523002
type: ComponentDefinition
persistent identity: BBa K523002
display id: BBa_K523002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + bglX (E. coli perisplasmic β-glucosidase)
creator: Sylvia Ispasanie, Mun Ching Lee
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the ''E. coli'' beta-glucosidase gene ''bglX''. The part contains the native Ribosome Binding Site. The part was made using the strategy outlined in BBa_K523000 , and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
mutable notes: The part was made using the strategy outlined in BBa_K523000 , and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. The part was created from a lab ''E. coli'' strain using primers: *Forward: ACT AGATCT gcc acg tcg ggc aac **adds BglII site; starts upstream of gene to catch RBS; RFC10 compliant after replacement of BBa_K523000 in a normal BioBrick vector. *Reverse: ACT ACTAGT A TTA tta cag caa ctc aaa ctc gcc **adds SpeI site; RFC10 compliant after insertion into vector; has double stop codon.
mutable provenance: The source ''E. coli'' genome sequence can be seen using GenBank U00096.2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: G to T silent mutation
sequence annotation: Native RBS
sequence annotation: bglX
sequence annotation: Predicted (cleaved) signal peptide
sequence annotation: PstI site removed
sequence annotation: TAATAA
sequence annotation: BglII
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K523002_sequence/1

G to T silent mutation
type: SequenceAnnotation
persistent identity: annotation2123636
display id: annotation2123636
version: 1
top level: BBa_K523002
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K523002/annotation2123636/range2123636/1

https://synbiohub.org/public/igem/BBa_K523002/annotation2123636/range2123636/1
type: Range
persistent identity: range2123636
display id: range2123636
version: 1
direction: direction/forward
top level: BBa_K523002
start: 2122
end: 2122
orientation: inline

Native RBS
type: SequenceAnnotation
persistent identity: annotation2123631
display id: annotation2123631
version: 1
top level: BBa_K523002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523002/annotation2123631/range2123631/1

https://synbiohub.org/public/igem/BBa_K523002/annotation2123631/range2123631/1
type: Range
persistent identity: range2123631
display id: range2123631
version: 1
direction: direction/forward
top level: BBa_K523002
start: 22
end: 25
orientation: inline

bglX
type: SequenceAnnotation
persistent identity: annotation2123632
display id: annotation2123632
version: 1
top level: BBa_K523002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523002/annotation2123632/range2123632/1

https://synbiohub.org/public/igem/BBa_K523002/annotation2123632/range2123632/1
type: Range
persistent identity: range2123632
display id: range2123632
version: 1
direction: direction/forward
top level: BBa_K523002
start: 38
end: 2338
orientation: inline

Predicted (cleaved) signal peptide
type: SequenceAnnotation
persistent identity: annotation2123640
display id: annotation2123640
version: 1
top level: BBa_K523002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523002/annotation2123640/range2123640/1

https://synbiohub.org/public/igem/BBa_K523002/annotation2123640/range2123640/1
type: Range
persistent identity: range2123640
display id: range2123640
version: 1
direction: direction/forward
top level: BBa_K523002
start: 38
end: 97
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2123635
display id: annotation2123635
version: 1
top level: BBa_K523002
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K523002/annotation2123635/range2123635/1

https://synbiohub.org/public/igem/BBa_K523002/annotation2123635/range2123635/1
type: Range
persistent identity: range2123635
display id: range2123635
version: 1
direction: direction/forward
top level: BBa_K523002
start: 85
end: 85
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2123634
display id: annotation2123634
version: 1
top level: BBa_K523002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523002/annotation2123634/range2123634/1

https://synbiohub.org/public/igem/BBa_K523002/annotation2123634/range2123634/1
type: Range
persistent identity: range2123634
display id: range2123634
version: 1
direction: direction/forward
top level: BBa_K523002
start: 2333
end: 2333
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation2123630
display id: annotation2123630
version: 1
top level: BBa_K523002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523002/annotation2123630/range2123630/1

https://synbiohub.org/public/igem/BBa_K523002/annotation2123630/range2123630/1
type: Range
persistent identity: range2123630
display id: range2123630
version: 1
direction: direction/forward
top level: BBa_K523002
start: 1
end: 4
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2123633
display id: annotation2123633
version: 1
top level: BBa_K523002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523002/annotation2123633/range2123633/1

https://synbiohub.org/public/igem/BBa_K523002/annotation2123633/range2123633/1
type: Range
persistent identity: range2123633
display id: range2123633
version: 1
direction: direction/forward
top level: BBa_K523002
start: 38
end: 38
orientation: inline

BBa_K575015
type: ComponentDefinition
persistent identity: BBa K575015
display id: BBa_K575015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP
creator: Kristin Palarz
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR/PAI2 inducable promoter with RBS (B0034) and RFP.
mutable notes: Need to ensure no leaky transcription.
mutable provenance: From BioBrick Parts Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0071
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0071
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K575015_sequence/1

BBa_R0071
type: Component
persistent identity: component2247803
display id: component2247803
version: 1
top level: BBa_K575015
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_E1010
type: Component
persistent identity: component2247808
display id: component2247808
version: 1
top level: BBa_K575015
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component2247805
display id: component2247805
version: 1
top level: BBa_K575015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2247805
display id: annotation2247805
version: 1
top level: BBa_K575015
location: https://synbiohub.org/public/igem/BBa_K575015/annotation2247805/range2247805/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K575015/annotation2247805/range2247805/1
type: Range
persistent identity: range2247805
display id: range2247805
version: 1
direction: direction/reverse
top level: BBa_K575015
start: 62
end: 73
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2247803
display id: annotation2247803
version: 1
top level: BBa_K575015
location: https://synbiohub.org/public/igem/BBa_K575015/annotation2247803/range2247803/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K575015/annotation2247803/range2247803/1
type: Range
persistent identity: range2247803
display id: range2247803
version: 1
direction: direction/reverse
top level: BBa_K575015
start: 1
end: 53
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247808
display id: annotation2247808
version: 1
top level: BBa_K575015
location: https://synbiohub.org/public/igem/BBa_K575015/annotation2247808/range2247808/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K575015/annotation2247808/range2247808/1
type: Range
persistent identity: range2247808
display id: range2247808
version: 1
direction: direction/reverse
top level: BBa_K575015
start: 80
end: 785
orientation: inline

BBa_K346004
type: ComponentDefinition
persistent identity: BBa K346004
display id: BBa_K346004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS(B0034)_MBP(lead metal binding peptide egineered from PbrR)+Terminator(B0015)
creator: Junyi Jiao
created: 2010-10-13T11:00:00Z
modified: 2016-01-13T10:29:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 6045
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is combined with a rbs, a mbp which codes for the metal binding peptide and a terminator.
mutable notes: to be given
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0015
sequence annotation: mbp(lead binding peptide)
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K346004_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2100341
display id: annotation2100341
version: 1
top level: BBa_K346004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100341/range2100341/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100341/range2100341/1
type: Range
persistent identity: range2100341
display id: range2100341
version: 1
direction: direction/forward
top level: BBa_K346004
start: 351
end: 479
orientation: inline

mbp(lead binding peptide)
type: SequenceAnnotation
persistent identity: annotation2100340
display id: annotation2100340
version: 1
top level: BBa_K346004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100340/range2100340/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100340/range2100340/1
type: Range
persistent identity: range2100340
display id: range2100340
version: 1
direction: direction/reverse
top level: BBa_K346004
start: 19
end: 345
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2100339
display id: annotation2100339
version: 1
top level: BBa_K346004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100339/range2100339/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100339/range2100339/1
type: Range
persistent identity: range2100339
display id: range2100339
version: 1
direction: direction/forward
top level: BBa_K346004
start: 5
end: 8
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2100338
display id: annotation2100338
version: 1
top level: BBa_K346004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100338/range2100338/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100338/range2100338/1
type: Range
persistent identity: range2100338
display id: range2100338
version: 1
direction: direction/forward
top level: BBa_K346004
start: 1
end: 12
orientation: inline

BBa_K608012
type: ComponentDefinition
persistent identity: BBa K608012
display id: BBa_K608012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium promoter with weak RBS and GFP
creator: Sandra Wassner
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 10299
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium promotor from the constitutive promotor family combined with a weak RBS and tagged with GFP to quantify the gene expression
mutable notes: We made this composite to test the effiency of promotor and RBS.
mutable provenance: Composite from registry parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23110
component: BBa_B0031
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_J23110
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_K608012_sequence/1

BBa_J23110
type: Component
persistent identity: component2128040
display id: component2128040
version: 1
top level: BBa_K608012
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0031
type: Component
persistent identity: component2128042
display id: component2128042
version: 1
top level: BBa_K608012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_E0040
type: Component
persistent identity: component2128044
display id: component2128044
version: 1
top level: BBa_K608012
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2128044
display id: annotation2128044
version: 1
top level: BBa_K608012
location: https://synbiohub.org/public/igem/BBa_K608012/annotation2128044/range2128044/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K608012/annotation2128044/range2128044/1
type: Range
persistent identity: range2128044
display id: range2128044
version: 1
direction: direction/reverse
top level: BBa_K608012
start: 64
end: 783
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2128040
display id: annotation2128040
version: 1
top level: BBa_K608012
location: https://synbiohub.org/public/igem/BBa_K608012/annotation2128040/range2128040/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K608012/annotation2128040/range2128040/1
type: Range
persistent identity: range2128040
display id: range2128040
version: 1
direction: direction/reverse
top level: BBa_K608012
start: 1
end: 35
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2128042
display id: annotation2128042
version: 1
top level: BBa_K608012
location: https://synbiohub.org/public/igem/BBa_K608012/annotation2128042/range2128042/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K608012/annotation2128042/range2128042/1
type: Range
persistent identity: range2128042
display id: range2128042
version: 1
direction: direction/reverse
top level: BBa_K608012
start: 44
end: 57
orientation: inline

BBa_K539642
type: ComponentDefinition
persistent identity: BBa K539642
display id: BBa_K539642
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a RBS (B0030)+ilvC with a RBS (B0030)+ilvD
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K539624
component: BBa_K539621
sequence annotation: BBa_K539624
sequence annotation: BBa_K539621
sequence: https://synbiohub.org/public/igem/BBa_K539642_sequence/1

BBa_K539624
type: Component
persistent identity: component2143951
display id: component2143951
version: 1
top level: BBa_K539642
access: public
definition: https://synbiohub.org/public/igem/BBa_K539624/1

BBa_K539621
type: Component
persistent identity: component2143945
display id: component2143945
version: 1
top level: BBa_K539642
access: public
definition: BBa_K539621

BBa_K539624
type: SequenceAnnotation
persistent identity: annotation2143951
display id: annotation2143951
version: 1
top level: BBa_K539642
location: https://synbiohub.org/public/igem/BBa_K539642/annotation2143951/range2143951/1
component: BBa_K539624

https://synbiohub.org/public/igem/BBa_K539642/annotation2143951/range2143951/1
type: Range
persistent identity: range2143951
display id: range2143951
version: 1
direction: direction/reverse
top level: BBa_K539642
start: 1506
end: 3377
orientation: inline

BBa_K539621
type: SequenceAnnotation
persistent identity: annotation2143945
display id: annotation2143945
version: 1
top level: BBa_K539642
location: https://synbiohub.org/public/igem/BBa_K539642/annotation2143945/range2143945/1
component: BBa_K539621

https://synbiohub.org/public/igem/BBa_K539642/annotation2143945/range2143945/1
type: Range
persistent identity: range2143945
display id: range2143945
version: 1
direction: direction/reverse
top level: BBa_K539642
start: 1
end: 1497
orientation: inline

BFP
type: ComponentDefinition
persistent identity: BBa K592100
display id: BBa_K592100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue Fluorescent Protein (mTagBFP)
creator: Erik Gullberg
created: 2011-09-19T11:00:00Z
modified: 2016-01-25T04:45:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 4206
owner id: 10137
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore. Subach et. al. started with a red fluorescent protein (TagRed) and converted it to a bright blue monomeric protein. See the article listed in source. The sequence has been codon optimized for expression in E coli by DNA 2.0.
mutable notes: The sequence has been codon optimized for expression in E coli by DNA 2.0.
mutable provenance: Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mTagBFP
sequence: https://synbiohub.org/public/igem/BBa_K592100_sequence/1

mTagBFP
type: SequenceAnnotation
persistent identity: annotation2135490
display id: annotation2135490
version: 1
top level: BFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592100/annotation2135490/range2135490/1

https://synbiohub.org/public/igem/BBa_K592100/annotation2135490/range2135490/1
type: Range
persistent identity: range2135490
display id: range2135490
version: 1
direction: direction/forward
top level: BFP
start: 1
end: 699
orientation: inline

BBa_K611059
type: ComponentDefinition
persistent identity: BBa K611059
display id: BBa_K611059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet Promoter Variant #9 in Characterization Construct
creator: Nicholas Csicsery
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains a variant of the wild type Tet repressible promoter, and is followed by the pTet characterization construct, K611013. This allows for testing of this particular mutant promoter at various concentrations of repressor. The mutated pTet promoter in this plasmid expresses GFP and is followed by an arabinose inducible promoter, pBad, which expresses the TetR repressor. Thus, a user can increase the concentration of repressor by inducing pBad with arabinose. This plasmid is very useful for the further characterization of K611049.
mutable notes: This part does not have a termination sequence at the end. It is recommended that for optimal results, this part be used in a backbone that includes a terminator (such as pSB1C3).
mutable provenance: Mutagenic PCR of the Tet repressible promoter, R0040, and standard BioBrick assembly of parts from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K611049
component: BBa_K611012
sequence annotation: BBa_K611049
sequence annotation: BBa_K611012
sequence: https://synbiohub.org/public/igem/BBa_K611059_sequence/1

BBa_K611049
type: Component
persistent identity: component2273691
display id: component2273691
version: 1
top level: BBa_K611059
access: public
definition: https://synbiohub.org/public/igem/BBa_K611049/1

BBa_K611012
type: Component
persistent identity: component2273710
display id: component2273710
version: 1
top level: BBa_K611059
access: public
definition: BBa_K611012

BBa_K611049
type: SequenceAnnotation
persistent identity: annotation2273691
display id: annotation2273691
version: 1
top level: BBa_K611059
location: https://synbiohub.org/public/igem/BBa_K611059/annotation2273691/range2273691/1
component: BBa_K611049

https://synbiohub.org/public/igem/BBa_K611059/annotation2273691/range2273691/1
type: Range
persistent identity: range2273691
display id: range2273691
version: 1
direction: direction/reverse
top level: BBa_K611059
start: 1
end: 54
orientation: inline

BBa_K611012
type: SequenceAnnotation
persistent identity: annotation2273710
display id: annotation2273710
version: 1
top level: BBa_K611059
location: https://synbiohub.org/public/igem/BBa_K611059/annotation2273710/range2273710/1
component: BBa_K611012

https://synbiohub.org/public/igem/BBa_K611059/annotation2273710/range2273710/1
type: Range
persistent identity: range2273710
display id: range2273710
version: 1
direction: direction/reverse
top level: BBa_K611059
start: 63
end: 1786
orientation: inline

BBa_K606048
type: ComponentDefinition
persistent identity: BBa K606048
display id: BBa_K606048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pHyperspank Tyrosine amber supressor tRNA
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tyrosine amber supressor tRNA expression system induced by IPTG in presence of LacI
mutable notes: biobrick assembly
mutable provenance: B. subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K606040
component: BBa_K606034
sequence annotation: BBa_K606034
sequence annotation: BBa_K606040
sequence: https://synbiohub.org/public/igem/BBa_K606048_sequence/1

BBa_K606040
type: Component
persistent identity: component2138069
display id: component2138069
version: 1
top level: BBa_K606048
access: public
definition: pHS

BBa_K606034
type: Component
persistent identity: component2138070
display id: component2138070
version: 1
top level: BBa_K606048
access: public
definition: Y taRNA

BBa_K606034
type: SequenceAnnotation
persistent identity: annotation2138070
display id: annotation2138070
version: 1
top level: BBa_K606048
location: https://synbiohub.org/public/igem/BBa_K606048/annotation2138070/range2138070/1
component: BBa_K606034

https://synbiohub.org/public/igem/BBa_K606048/annotation2138070/range2138070/1
type: Range
persistent identity: range2138070
display id: range2138070
version: 1
direction: direction/reverse
top level: BBa_K606048
start: 101
end: 185
orientation: inline

BBa_K606040
type: SequenceAnnotation
persistent identity: annotation2138069
display id: annotation2138069
version: 1
top level: BBa_K606048
location: https://synbiohub.org/public/igem/BBa_K606048/annotation2138069/range2138069/1
component: BBa_K606040

https://synbiohub.org/public/igem/BBa_K606048/annotation2138069/range2138069/1
type: Range
persistent identity: range2138069
display id: range2138069
version: 1
direction: direction/reverse
top level: BBa_K606048
start: 1
end: 92
orientation: inline

Cpn10
type: ComponentDefinition
persistent identity: BBa K538000
display id: BBa_K538000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cpn10 (O. antarctica)
creator: Bas Stringer (Sequence by Paul van Dieken)
created: 2011-07-27T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part encodes the protein ''cochaperonin 10'', which is part of the Cpn10/Cpn60 chaperonin system of ''Oleispira antarctica''. [More information will follow soon.]
mutable notes: The protein's sequence[1] was published by Ferrer ''et al.'' in 2004. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[2] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[3] was confirmed using the EMBOSS recoder[4], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI. 1 - http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts 2 - http://openwetware.org/wiki/Biobrick_standard 3 - http://partsregistry.org/Help:Assembly_standard_10 4 - http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html
mutable provenance: ''De novo'' synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K538000_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2124174
display id: annotation2124174
version: 1
top level: Cpn10
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K538000/annotation2124174/range2124174/1

https://synbiohub.org/public/igem/BBa_K538000/annotation2124174/range2124174/1
type: Range
persistent identity: range2124174
display id: range2124174
version: 1
direction: direction/forward
top level: Cpn10
start: 1
end: 3
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2124175
display id: annotation2124175
version: 1
top level: Cpn10
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K538000/annotation2124175/range2124175/1

https://synbiohub.org/public/igem/BBa_K538000/annotation2124175/range2124175/1
type: Range
persistent identity: range2124175
display id: range2124175
version: 1
direction: direction/forward
top level: Cpn10
start: 292
end: 297
orientation: inline

BBa_K331022
type: ComponentDefinition
persistent identity: BBa K331022
display id: BBa_K331022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS with N-terminal Oligo Arginine - EYFP Fusion
creator: Adam Smith
created: 2010-09-28T11:00:00Z
modified: 2015-05-08T01:12:04Z
discontinued: false
dominant: false
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has a oligo arginine sequence fused to the N-terminus of an enhanced yellow fluorescent protein. This will be a component of the proof-of-concept part K331018 which will show localization of proteins into the interior of a microcompartment formed by the assemly of lumazine synthase proteins.
mutable notes: We chose not to include a transcriptional promoter to this part because we wanted the freedom to put it under the control of varied promoters. We also chose not to include a transcriptional terminator to this part because we planned to use it in an operon, where we would be expressing multiple proteins from a single transcript.
mutable provenance: This sequence was synthesized by Gene Art
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K249008
sequence annotation: BBa_B0034
sequence annotation: BBa_K249008
sequence: https://synbiohub.org/public/igem/BBa_K331022_sequence/1

BBa_B0034
type: Component
persistent identity: component2081107
display id: component2081107
version: 1
top level: BBa_K331022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K249008
type: Component
persistent identity: component2081108
display id: component2081108
version: 1
top level: BBa_K331022
access: public
definition: https://synbiohub.org/public/igem/BBa_K249008/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2081107
display id: annotation2081107
version: 1
top level: BBa_K331022
location: https://synbiohub.org/public/igem/BBa_K331022/annotation2081107/range2081107/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331022/annotation2081107/range2081107/1
type: Range
persistent identity: range2081107
display id: range2081107
version: 1
direction: direction/reverse
top level: BBa_K331022
start: 1
end: 12
orientation: inline

BBa_K249008
type: SequenceAnnotation
persistent identity: annotation2081108
display id: annotation2081108
version: 1
top level: BBa_K331022
location: https://synbiohub.org/public/igem/BBa_K331022/annotation2081108/range2081108/1
component: BBa_K249008

https://synbiohub.org/public/igem/BBa_K331022/annotation2081108/range2081108/1
type: Range
persistent identity: range2081108
display id: range2081108
version: 1
direction: direction/reverse
top level: BBa_K331022
start: 19
end: 780
orientation: inline

BBa_K823017
type: ComponentDefinition
persistent identity: BBa K823017
display id: BBa_K823017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0012-B0011)
creator: Jara Radeck
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
mutable notes: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
mutable provenance: Part:BBa_B0014
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0014
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K823017_sequence/1

BBa_B0014
type: Component
persistent identity: component2182657
display id: component2182657
version: 1
top level: BBa_K823017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2182657
display id: annotation2182657
version: 1
top level: BBa_K823017
location: https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
type: Range
persistent identity: range2182657
display id: range2182657
version: 1
direction: direction/reverse
top level: BBa_K823017
start: 1
end: 95
orientation: inline

BBa_K864600
type: ComponentDefinition
persistent identity: BBa K864600
display id: BBa_K864600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T0 (21 imm) transcriptional terminator
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The T0 transcriptional terminator terminates transcription with 80-90% efficiency.
mutable notes: -
mutable provenance: Gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T0 terminator
sequence: https://synbiohub.org/public/igem/BBa_K864600_sequence/1

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2197273
display id: annotation2197273
version: 1
top level: BBa_K864600
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864600/annotation2197273/range2197273/1

https://synbiohub.org/public/igem/BBa_K864600/annotation2197273/range2197273/1
type: Range
persistent identity: range2197273
display id: range2197273
version: 1
direction: direction/forward
top level: BBa_K864600
start: 1
end: 52
orientation: inline

BBa_K733013
type: ComponentDefinition
persistent identity: BBa K733013
display id: BBa_K733013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg+R.B.S.
creator: Chris, Yu lai cheong
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K316001
component: BBa_K143021
sequence annotation: BBa_K316001
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K733013_sequence/1

BBa_K316001
type: Component
persistent identity: component2183794
display id: component2183794
version: 1
top level: BBa_K733013
access: public
definition: https://synbiohub.org/public/igem/BBa_K316001/1

BBa_K143021
type: Component
persistent identity: component2183796
display id: component2183796
version: 1
top level: BBa_K733013
access: public
definition: https://synbiohub.org/public/igem/BBa_K143021/1

BBa_K316001
type: SequenceAnnotation
persistent identity: annotation2183794
display id: annotation2183794
version: 1
top level: BBa_K733013
location: https://synbiohub.org/public/igem/BBa_K733013/annotation2183794/range2183794/1
component: BBa_K316001

https://synbiohub.org/public/igem/BBa_K733013/annotation2183794/range2183794/1
type: Range
persistent identity: range2183794
display id: range2183794
version: 1
direction: direction/reverse
top level: BBa_K733013
start: 1
end: 97
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2183796
display id: annotation2183796
version: 1
top level: BBa_K733013
location: https://synbiohub.org/public/igem/BBa_K733013/annotation2183796/range2183796/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K733013/annotation2183796/range2183796/1
type: Range
persistent identity: range2183796
display id: range2183796
version: 1
direction: direction/reverse
top level: BBa_K733013
start: 98
end: 109
orientation: inline

WT CysE
type: ComponentDefinition
persistent identity: BBa K731000
display id: BBa_K731000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CysE (Serine Acetyltransferase)
creator: Jason Fontana, Daniele Rossetto, Giacomo Giacomelli
created: 2012-07-25T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.
mutable notes: The construct has a strong RBS (AGGAGG) spaced 8nt from the start codon.
mutable provenance: E. coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: spacer
sequence annotation: RBS
sequence annotation: Stop Codons
sequence: https://synbiohub.org/public/igem/BBa_K731000_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2180203
display id: annotation2180203
version: 1
top level: WT CysE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K731000/annotation2180203/range2180203/1

https://synbiohub.org/public/igem/BBa_K731000/annotation2180203/range2180203/1
type: Range
persistent identity: range2180203
display id: range2180203
version: 1
direction: direction/forward
top level: WT CysE
start: 15
end: 17
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation2178419
display id: annotation2178419
version: 1
top level: WT CysE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731000/annotation2178419/range2178419/1

https://synbiohub.org/public/igem/BBa_K731000/annotation2178419/range2178419/1
type: Range
persistent identity: range2178419
display id: range2178419
version: 1
direction: direction/forward
top level: WT CysE
start: 7
end: 14
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2178418
display id: annotation2178418
version: 1
top level: WT CysE
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K731000/annotation2178418/range2178418/1

https://synbiohub.org/public/igem/BBa_K731000/annotation2178418/range2178418/1
type: Range
persistent identity: range2178418
display id: range2178418
version: 1
direction: direction/forward
top level: WT CysE
start: 1
end: 6
orientation: inline

Stop Codons
type: SequenceAnnotation
persistent identity: annotation2178420
display id: annotation2178420
version: 1
top level: WT CysE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K731000/annotation2178420/range2178420/1

https://synbiohub.org/public/igem/BBa_K731000/annotation2178420/range2178420/1
type: Range
persistent identity: range2178420
display id: range2178420
version: 1
direction: direction/forward
top level: WT CysE
start: 834
end: 839
orientation: inline

BBa_K575031
type: ComponentDefinition
persistent identity: BBa K575031
display id: BBa_K575031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP, Constitutive Promoter + RBS (B0034) + LasR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of LasR combined with receptor
mutable notes: put the constitutive promoter after the production of LasR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575005
component: BBa_K575011
sequence annotation: BBa_K575005
sequence annotation: BBa_K575011
sequence: https://synbiohub.org/public/igem/BBa_K575031_sequence/1

BBa_K575005
type: Component
persistent identity: component2264177
display id: component2264177
version: 1
top level: BBa_K575031
access: public
definition: https://synbiohub.org/public/igem/BBa_K575005/1

BBa_K575011
type: Component
persistent identity: component2264172
display id: component2264172
version: 1
top level: BBa_K575031
access: public
definition: BBa_K575011

BBa_K575005
type: SequenceAnnotation
persistent identity: annotation2264177
display id: annotation2264177
version: 1
top level: BBa_K575031
location: https://synbiohub.org/public/igem/BBa_K575031/annotation2264177/range2264177/1
component: BBa_K575005

https://synbiohub.org/public/igem/BBa_K575031/annotation2264177/range2264177/1
type: Range
persistent identity: range2264177
display id: range2264177
version: 1
direction: direction/reverse
top level: BBa_K575031
start: 898
end: 1681
orientation: inline

BBa_K575011
type: SequenceAnnotation
persistent identity: annotation2264172
display id: annotation2264172
version: 1
top level: BBa_K575031
location: https://synbiohub.org/public/igem/BBa_K575031/annotation2264172/range2264172/1
component: BBa_K575011

https://synbiohub.org/public/igem/BBa_K575031/annotation2264172/range2264172/1
type: Range
persistent identity: range2264172
display id: range2264172
version: 1
direction: direction/reverse
top level: BBa_K575031
start: 1
end: 889
orientation: inline

PT5lac
type: ComponentDefinition
persistent identity: BBa K592008
display id: BBa_K592008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T5-lac Promoter
creator: Erik Lundin
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E.coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase.
mutable notes: The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.
mutable provenance: Phage T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T5-lac promoter
sequence annotation: -10 signal
sequence annotation: -35 signal
sequence annotation: lacO1 site
sequence annotation: lacOi site
sequence: https://synbiohub.org/public/igem/BBa_K592008_sequence/1

T5-lac promoter
type: SequenceAnnotation
persistent identity: annotation2127135
display id: annotation2127135
version: 1
top level: PT5lac
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2127135/range2127135/1

https://synbiohub.org/public/igem/BBa_K592008/annotation2127135/range2127135/1
type: Range
persistent identity: range2127135
display id: range2127135
version: 1
direction: direction/forward
top level: PT5lac
start: 1
end: 126
orientation: inline

-10 signal
type: SequenceAnnotation
persistent identity: annotation2135661
display id: annotation2135661
version: 1
top level: PT5lac
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135661/range2135661/1

https://synbiohub.org/public/igem/BBa_K592008/annotation2135661/range2135661/1
type: Range
persistent identity: range2135661
display id: range2135661
version: 1
direction: direction/forward
top level: PT5lac
start: 87
end: 93
orientation: inline

-35 signal
type: SequenceAnnotation
persistent identity: annotation2135659
display id: annotation2135659
version: 1
top level: PT5lac
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135659/range2135659/1

https://synbiohub.org/public/igem/BBa_K592008/annotation2135659/range2135659/1
type: Range
persistent identity: range2135659
display id: range2135659
version: 1
direction: direction/forward
top level: PT5lac
start: 63
end: 68
orientation: inline

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2135657
display id: annotation2135657
version: 1
top level: PT5lac
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135657/range2135657/1

https://synbiohub.org/public/igem/BBa_K592008/annotation2135657/range2135657/1
type: Range
persistent identity: range2135657
display id: range2135657
version: 1
direction: direction/forward
top level: PT5lac
start: 69
end: 86
orientation: inline

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2135656
display id: annotation2135656
version: 1
top level: PT5lac
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135656/range2135656/1

https://synbiohub.org/public/igem/BBa_K592008/annotation2135656/range2135656/1
type: Range
persistent identity: range2135656
display id: range2135656
version: 1
direction: direction/forward
top level: PT5lac
start: 1
end: 26
orientation: inline

lacOi site
type: SequenceAnnotation
persistent identity: annotation2135658
display id: annotation2135658
version: 1
top level: PT5lac
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K592008/annotation2135658/range2135658/1

https://synbiohub.org/public/igem/BBa_K592008/annotation2135658/range2135658/1
type: Range
persistent identity: range2135658
display id: range2135658
version: 1
direction: direction/forward
top level: PT5lac
start: 94
end: 123
orientation: inline

BBa_K747096
type: ComponentDefinition
persistent identity: BBa K747096
display id: BBa_K747096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV-promoter
creator: Lucas Schneider
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: c
mutable provenance: not sure
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K747096_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2199147
display id: annotation2199147
version: 1
top level: BBa_K747096
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K747096/annotation2199147/range2199147/1

https://synbiohub.org/public/igem/BBa_K747096/annotation2199147/range2199147/1
type: Range
persistent identity: range2199147
display id: range2199147
version: 1
direction: direction/forward
top level: BBa_K747096
start: 1
end: 580
orientation: inline

lexA
type: ComponentDefinition
persistent identity: BBa K864201
display id: BBa_K864201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA SOS response repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lexA is important in induction of recA
mutable notes: None
mutable provenance: Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined) lexA_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAGCGTTAACGGCCAG lexA_BB_R CGGCCGCTACTAGTATTATTACAGCCAGTCGCCGTT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lexA
sequence: https://synbiohub.org/public/igem/BBa_K864201_sequence/1

lexA
type: SequenceAnnotation
persistent identity: annotation2197113
display id: annotation2197113
version: 1
top level: lexA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864201/annotation2197113/range2197113/1

https://synbiohub.org/public/igem/BBa_K864201/annotation2197113/range2197113/1
type: Range
persistent identity: range2197113
display id: range2197113
version: 1
direction: direction/forward
top level: lexA
start: 1
end: 612
orientation: inline

BBa_K516132
type: ComponentDefinition
persistent identity: BBa K516132
display id: BBa_K516132
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter [J23101] with mRFP, RBS B0032
creator: Susanna Zucca, Federica Sampietro, Giuseppe Bertoni
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Constitutive promoter [J23101] with mRFP, RBS B0032
mutable notes: --
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E1010
component: BBa_B0032
component: BBa_B0015
component: BBa_J23101
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: BBa_J23101
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K516132_sequence/1

BBa_E1010
type: Component
persistent identity: component2258488
display id: component2258488
version: 1
top level: BBa_K516132
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0032
type: Component
persistent identity: component2258484
display id: component2258484
version: 1
top level: BBa_K516132
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0015
type: Component
persistent identity: component2258495
display id: component2258495
version: 1
top level: BBa_K516132
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J23101
type: Component
persistent identity: component2258482
display id: component2258482
version: 1
top level: BBa_K516132
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2258488
display id: annotation2258488
version: 1
top level: BBa_K516132
location: https://synbiohub.org/public/igem/BBa_K516132/annotation2258488/range2258488/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K516132/annotation2258488/range2258488/1
type: Range
persistent identity: range2258488
display id: range2258488
version: 1
direction: direction/reverse
top level: BBa_K516132
start: 63
end: 768
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2258495
display id: annotation2258495
version: 1
top level: BBa_K516132
location: https://synbiohub.org/public/igem/BBa_K516132/annotation2258495/range2258495/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516132/annotation2258495/range2258495/1
type: Range
persistent identity: range2258495
display id: range2258495
version: 1
direction: direction/reverse
top level: BBa_K516132
start: 777
end: 905
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2258482
display id: annotation2258482
version: 1
top level: BBa_K516132
location: https://synbiohub.org/public/igem/BBa_K516132/annotation2258482/range2258482/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K516132/annotation2258482/range2258482/1
type: Range
persistent identity: range2258482
display id: range2258482
version: 1
direction: direction/reverse
top level: BBa_K516132
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2258484
display id: annotation2258484
version: 1
top level: BBa_K516132
location: https://synbiohub.org/public/igem/BBa_K516132/annotation2258484/range2258484/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K516132/annotation2258484/range2258484/1
type: Range
persistent identity: range2258484
display id: range2258484
version: 1
direction: direction/forward
top level: BBa_K516132
start: 44
end: 56
orientation: inline

flhDC
type: ComponentDefinition
persistent identity: BBa K554001
display id: BBa_K554001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC promoter
creator: UNICAMP EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: flhDC is ...
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: flhDC
sequence: https://synbiohub.org/public/igem/BBa_K554001_sequence/1

flhDC
type: SequenceAnnotation
persistent identity: annotation2136874
display id: annotation2136874
version: 1
top level: flhDC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K554001/annotation2136874/range2136874/1

https://synbiohub.org/public/igem/BBa_K554001/annotation2136874/range2136874/1
type: Range
persistent identity: range2136874
display id: range2136874
version: 1
direction: direction/forward
top level: flhDC
start: 1
end: 85
orientation: inline

BBa_K518012
type: ComponentDefinition
persistent identity: BBa K518012
display id: BBa_K518012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + RFP + d.Ter
creator: Masato Ohgishi
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an RFP coding device. When ligated with a promoter, it will yield a red colony so that you can determine whether your promoter works.
mutable notes: None.
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0032
component: BBa_E1010
component: BBa_B0014
sequence annotation: BBa_B0032
sequence annotation: BBa_B0014
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K518012_sequence/1

BBa_B0032
type: Component
persistent identity: component2250776
display id: component2250776
version: 1
top level: BBa_K518012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E1010
type: Component
persistent identity: component2250780
display id: component2250780
version: 1
top level: BBa_K518012
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0014
type: Component
persistent identity: component2250787
display id: component2250787
version: 1
top level: BBa_K518012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250776
display id: annotation2250776
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250776/range2250776/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518012/annotation2250776/range2250776/1
type: Range
persistent identity: range2250776
display id: range2250776
version: 1
direction: direction/forward
top level: BBa_K518012
start: 1
end: 13
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2250787
display id: annotation2250787
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250787/range2250787/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518012/annotation2250787/range2250787/1
type: Range
persistent identity: range2250787
display id: range2250787
version: 1
direction: direction/reverse
top level: BBa_K518012
start: 734
end: 828
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250780
display id: annotation2250780
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250780/range2250780/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K518012/annotation2250780/range2250780/1
type: Range
persistent identity: range2250780
display id: range2250780
version: 1
direction: direction/reverse
top level: BBa_K518012
start: 20
end: 725
orientation: inline

BBa_K817023
type: ComponentDefinition
persistent identity: BBa K817023
display id: BBa_K817023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-SP3-Penetratin-Flag
creator: Jui-Tsen Yu
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1075_
m user id: 0
owner id: 13572
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: RBS-SP3-xx
mutable notes: RBS-SP3-xx
mutable provenance: RBS-SP3-xx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0030
component: BBa_K817029
sequence annotation: BBa_K817029
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K817023_sequence/1

BBa_B0030
type: Component
persistent identity: component2201542
display id: component2201542
version: 1
top level: BBa_K817023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K817029
type: Component
persistent identity: component2201547
display id: component2201547
version: 1
top level: BBa_K817023
access: public
definition: https://synbiohub.org/public/igem/BBa_K817029/1

BBa_K817029
type: SequenceAnnotation
persistent identity: annotation2201547
display id: annotation2201547
version: 1
top level: BBa_K817023
location: https://synbiohub.org/public/igem/BBa_K817023/annotation2201547/range2201547/1
component: BBa_K817029

https://synbiohub.org/public/igem/BBa_K817023/annotation2201547/range2201547/1
type: Range
persistent identity: range2201547
display id: range2201547
version: 1
direction: direction/reverse
top level: BBa_K817023
start: 22
end: 168
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2201542
display id: annotation2201542
version: 1
top level: BBa_K817023
location: https://synbiohub.org/public/igem/BBa_K817023/annotation2201542/range2201542/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K817023/annotation2201542/range2201542/1
type: Range
persistent identity: range2201542
display id: range2201542
version: 1
direction: direction/forward
top level: BBa_K817023
start: 1
end: 15
orientation: inline

BBa_K648028
type: ComponentDefinition
persistent identity: BBa K648028
display id: BBa_K648028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cro, Lamda Repressor which activates the lytic cycle
creator: James Coletta, Anisha Katyal, Kristen Salavo, Lauren Rossi
created: 2011-07-24T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9891
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cro is produced by the pr promoter in the lambda phage system. Normally C1 is repressing the pr promoter. However, when the lytic switch is thrown and RecA cleaves the C1 dimer the pr promoter is unrepressed. This results in the production of cro which in turn represses the prm promoter which halts the transcription of C1 repressor. In our project we used Cro to assemble our lambda system.
mutable provenance: Galen Lynch's notebook (Penn State iGEM 2007 member)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K648028_sequence/1

BBa_K823012
type: ComponentDefinition
persistent identity: BBa K823012
display id: BBa_K823012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23115
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23115 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23115
sequence: https://synbiohub.org/public/igem/BBa_K823012_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182541
display id: annotation2182541
version: 1
top level: BBa_K823012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823012/annotation2182541/range2182541/1

https://synbiohub.org/public/igem/BBa_K823012/annotation2182541/range2182541/1
type: Range
persistent identity: range2182541
display id: range2182541
version: 1
direction: direction/forward
top level: BBa_K823012
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182542
display id: annotation2182542
version: 1
top level: BBa_K823012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823012/annotation2182542/range2182542/1

https://synbiohub.org/public/igem/BBa_K823012/annotation2182542/range2182542/1
type: Range
persistent identity: range2182542
display id: range2182542
version: 1
direction: direction/forward
top level: BBa_K823012
start: 24
end: 29
orientation: inline

J23115
type: SequenceAnnotation
persistent identity: annotation2182540
display id: annotation2182540
version: 1
top level: BBa_K823012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823012/annotation2182540/range2182540/1

https://synbiohub.org/public/igem/BBa_K823012/annotation2182540/range2182540/1
type: Range
persistent identity: range2182540
display id: range2182540
version: 1
direction: direction/forward
top level: BBa_K823012
start: 1
end: 35
orientation: inline

BBa_K523015
type: ComponentDefinition
persistent identity: BBa K523015
display id: BBa_K523015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac + RBS + cenA (endoglucanase)
creator: Edinburgh 2008, 2011
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is ''C. fimi'' endoglucanase under the control of the Lac promoter.
mutable notes: By assembly of specified parts.
mutable provenance: By assembly of specified parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15001
component: BBa_K118023
component: BBa_J33207
sequence annotation: BBa_J15001
sequence annotation: BBa_K118023
sequence annotation: BBa_J33207
sequence: https://synbiohub.org/public/igem/BBa_K523015_sequence/1

BBa_J15001
type: Component
persistent identity: component2127248
display id: component2127248
version: 1
top level: BBa_K523015
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118023
type: Component
persistent identity: component2127250
display id: component2127250
version: 1
top level: BBa_K523015
access: public
definition: https://synbiohub.org/public/igem/BBa_K118023/1

BBa_J33207
type: Component
persistent identity: component2127245
display id: component2127245
version: 1
top level: BBa_K523015
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2127248
display id: annotation2127248
version: 1
top level: BBa_K523015
location: https://synbiohub.org/public/igem/BBa_K523015/annotation2127248/range2127248/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K523015/annotation2127248/range2127248/1
type: Range
persistent identity: range2127248
display id: range2127248
version: 1
direction: direction/reverse
top level: BBa_K523015
start: 609
end: 618
orientation: inline

BBa_K118023
type: SequenceAnnotation
persistent identity: annotation2127250
display id: annotation2127250
version: 1
top level: BBa_K523015
location: https://synbiohub.org/public/igem/BBa_K523015/annotation2127250/range2127250/1
component: BBa_K118023

https://synbiohub.org/public/igem/BBa_K523015/annotation2127250/range2127250/1
type: Range
persistent identity: range2127250
display id: range2127250
version: 1
direction: direction/reverse
top level: BBa_K523015
start: 625
end: 1977
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2127245
display id: annotation2127245
version: 1
top level: BBa_K523015
location: https://synbiohub.org/public/igem/BBa_K523015/annotation2127245/range2127245/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K523015/annotation2127245/range2127245/1
type: Range
persistent identity: range2127245
display id: range2127245
version: 1
direction: direction/reverse
top level: BBa_K523015
start: 1
end: 600
orientation: inline

BBa_K575029
type: ComponentDefinition
persistent identity: BBa K575029
display id: BBa_K575029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0034) + LasR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of LasR combined with receptor
mutable notes: put the constitutive promoter after the production of LasR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575009
component: BBa_K575005
sequence annotation: BBa_K575009
sequence annotation: BBa_K575005
sequence: https://synbiohub.org/public/igem/BBa_K575029_sequence/1

BBa_K575009
type: Component
persistent identity: component2138289
display id: component2138289
version: 1
top level: BBa_K575029
access: public
definition: BBa_K575009

BBa_K575005
type: Component
persistent identity: component2138294
display id: component2138294
version: 1
top level: BBa_K575029
access: public
definition: https://synbiohub.org/public/igem/BBa_K575005/1

BBa_K575009
type: SequenceAnnotation
persistent identity: annotation2138289
display id: annotation2138289
version: 1
top level: BBa_K575029
location: https://synbiohub.org/public/igem/BBa_K575029/annotation2138289/range2138289/1
component: BBa_K575009

https://synbiohub.org/public/igem/BBa_K575029/annotation2138289/range2138289/1
type: Range
persistent identity: range2138289
display id: range2138289
version: 1
direction: direction/reverse
top level: BBa_K575029
start: 1
end: 903
orientation: inline

BBa_K575005
type: SequenceAnnotation
persistent identity: annotation2138294
display id: annotation2138294
version: 1
top level: BBa_K575029
location: https://synbiohub.org/public/igem/BBa_K575029/annotation2138294/range2138294/1
component: BBa_K575005

https://synbiohub.org/public/igem/BBa_K575029/annotation2138294/range2138294/1
type: Range
persistent identity: range2138294
display id: range2138294
version: 1
direction: direction/reverse
top level: BBa_K575029
start: 912
end: 1695
orientation: inline

BBa_K817016
type: ComponentDefinition
persistent identity: BBa K817016
display id: BBa_K817016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: parS site
creator: Shan Chi Hsieh
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1075_
m user id: 0
owner id: 13960
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is the centromere site that can be recognized by parB of pseudomonas putida KT2440, when providing this part in cis on an low copy plasmid and providing parAB cassette in cis or in trans, the plasmid can be active partitioned thus it can enhances plasmid segregation stability and reduces plasmid loss rate.
mutable notes: It had better be used in low copy plasmid.
mutable provenance: pseudomonas putida KT2440 chromosome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: parB binding site
sequence: https://synbiohub.org/public/igem/BBa_K817016_sequence/1

parB binding site
type: SequenceAnnotation
persistent identity: annotation2187456
display id: annotation2187456
version: 1
top level: BBa_K817016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K817016/annotation2187456/range2187456/1

https://synbiohub.org/public/igem/BBa_K817016/annotation2187456/range2187456/1
type: Range
persistent identity: range2187456
display id: range2187456
version: 1
direction: direction/forward
top level: BBa_K817016
start: 1
end: 16
orientation: inline

BBa_K546003
type: ComponentDefinition
persistent identity: BBa K546003
display id: BBa_K546003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR + lux pR promoter.
creator: Brendan Ryback, Matthijn Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 8616
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part was made as a replacement for a defective version of BBa_F2621 which lacked the R0063 promoter, but was otherwise intact and functional. It shows a significantly higher response to 3OC6HSL than the incomplete BBa_F2621 (which is present in the Registry as BBa_K317037)
mutable notes: No specific considerations.
mutable provenance: Was constructed by cloning BBa_R0063 in front of the incomplete BBa_F2621 (sent from the Registry in July 2011 lacking the R0063 subcomponent)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K317037
component: BBa_R0063
sequence annotation: BBa_K317037
sequence annotation: BBa_R0063
sequence: https://synbiohub.org/public/igem/BBa_K546003_sequence/1

BBa_K317037
type: Component
persistent identity: component2264075
display id: component2264075
version: 1
top level: BBa_K546003
access: public
definition: https://synbiohub.org/public/igem/BBa_K317037/1

BBa_R0063
type: Component
persistent identity: component2264050
display id: component2264050
version: 1
top level: BBa_K546003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_K317037
type: SequenceAnnotation
persistent identity: annotation2264075
display id: annotation2264075
version: 1
top level: BBa_K546003
location: https://synbiohub.org/public/igem/BBa_K546003/annotation2264075/range2264075/1
component: BBa_K317037

https://synbiohub.org/public/igem/BBa_K546003/annotation2264075/range2264075/1
type: Range
persistent identity: range2264075
display id: range2264075
version: 1
direction: direction/reverse
top level: BBa_K546003
start: 160
end: 1158
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2264050
display id: annotation2264050
version: 1
top level: BBa_K546003
location: https://synbiohub.org/public/igem/BBa_K546003/annotation2264050/range2264050/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K546003/annotation2264050/range2264050/1
type: Range
persistent identity: range2264050
display id: range2264050
version: 1
direction: direction/forward
top level: BBa_K546003
start: 1
end: 151
orientation: inline

BBa_K851002
type: ComponentDefinition
persistent identity: BBa K851002
display id: BBa_K851002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD-pXyl AND Gate
creator: Abiel Trevi??o Garza
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1111_
m user id: 0
owner id: 12295
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: pBAD-pXyl is a combined promoter of D-Xylose and L-arabinose sugar sensor systems which is designed to be activated only in the presence of both sugars in the medium therefore functioning as an AND logic gate. BIOLOGY pXyl is an inducible promoter regulated by the transcriptional regulator XylR which, in Bacillus subtilis, regulates the expression of xyl operon[1]. Gene expression under pXyl can be induced by the addition of D-Xylose to the medium [1, 2]. The nucleotide sequence was obtained from Wilhelm, M &C. P. Hollenberg, 1985[3]. In the presence of L-arabinose, expression from pBAD is turned on while the absence of L-arabinose produces very low levels of transcription from pBAD [4, 5]. More precisely, in the absence of arabinose, the repressor protein AraC (BBa_I13458[6]) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription[7], but in the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription[7]. The nucleotide sequence was similar of that in Part:BBa_K206000[8]. For iGEM UNAM Genomics M??xico 2012 project [9], pBAD/pXyl was used in the design of an AND logic gate[10] using a recently described new type of communication system between Bacillus Subtilis cells called Nanotubes[11].
mutable notes: chimeric promotor
mutable provenance: REFERENCES [1] D Gartner, M Geissendorfer, & W Hillen(1988). Expression of the Bacillus subtilis xyl Operon Is Repressed at the Level of Transcription and Is Induced by Xylose J Bacteriol 170:7,3102-3109. [2] Shamanna, D. K., and K. E. Sanderson. 1979. Genetics and regulation of D-xylose utilization in Salmonella typhimurium LT2. J. Bacteriol. 139:71-79. [3] Wilhelm, M., and C. P. Hollenberg. 1985. Nucleotide sequence of the Bacillus subtilis xylose isomerase gene: extensive homology between the Bacillus and E. coli enzyme. Nucleic Acids Res. 13:5717-5722. [4] Lee, N. (1980) Molecular Aspects of ara Regulation. In The Operon, J. H. Miller and W. S. Reznikoff, eds. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory), pp. 389-410. [5] Lee, N., Francklyn, C., and Hamilton, E. P. (1987). Arabinose-Induced Binding of AraC Protein to araI2 Activates the araBAD Operon Promoter. Proc. Natl. Acad. Sci. USA 84, 8814-8818. [6] http://partsregistry.org/wiki/index.php/Part:BBa_I13458 [8] http://partsregistry.org/Part:BBa_K206000 [7] Schlief, R. (2000). Regulation of the L-arabinose operon of Escherichia coli. Trends in Genetics. 16(12):559-565. [9] http://2012.igem.org/Team:UNAM_Genomics_Mexico [10] http://2012.igem.org/Team:UNAM_Genomics_Mexico/Project/Description [11] Dubey GP, Ben-Yehuda S. (2011) Intercellular nanotubes mediate bacterial communication. Cell.;144(4):590-600 [12] Guzman, L.-M., Belin, D., Carson, M. J., and Beckwith, J. (1995). Tight Regulation, Modulation, and High-Level Expression by Vectors Containing the Arabinose PBAD Promoter. J. Bacteriol. 177, 4121-4130. [13] David, J. D., and H. Weismeyer. 1970. Control of xylose metabolism in Escherichia coli. Biochim. Biophys. Acta 201: 497-499.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: pBad recognition region 1
sequence annotation: Pbad recognition region 2
sequence annotation: XylR Binding Site 2
sequence annotation: -10
sequence annotation: XylR Binding Sites
sequence: https://synbiohub.org/public/igem/BBa_K851002_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2196287
display id: annotation2196287
version: 1
top level: BBa_K851002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K851002/annotation2196287/range2196287/1

https://synbiohub.org/public/igem/BBa_K851002/annotation2196287/range2196287/1
type: Range
persistent identity: range2196287
display id: range2196287
version: 1
direction: direction/forward
top level: BBa_K851002
start: 247
end: 253
orientation: inline

pBad recognition region 1
type: SequenceAnnotation
persistent identity: annotation2196277
display id: annotation2196277
version: 1
top level: BBa_K851002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K851002/annotation2196277/range2196277/1

https://synbiohub.org/public/igem/BBa_K851002/annotation2196277/range2196277/1
type: Range
persistent identity: range2196277
display id: range2196277
version: 1
direction: direction/forward
top level: BBa_K851002
start: 3
end: 19
orientation: inline

Pbad recognition region 2
type: SequenceAnnotation
persistent identity: annotation2196278
display id: annotation2196278
version: 1
top level: BBa_K851002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K851002/annotation2196278/range2196278/1

https://synbiohub.org/public/igem/BBa_K851002/annotation2196278/range2196278/1
type: Range
persistent identity: range2196278
display id: range2196278
version: 1
direction: direction/forward
top level: BBa_K851002
start: 161
end: 174
orientation: inline

XylR Binding Site 2
type: SequenceAnnotation
persistent identity: annotation2196302
display id: annotation2196302
version: 1
top level: BBa_K851002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K851002/annotation2196302/range2196302/1

https://synbiohub.org/public/igem/BBa_K851002/annotation2196302/range2196302/1
type: Range
persistent identity: range2196302
display id: range2196302
version: 1
direction: direction/forward
top level: BBa_K851002
start: 305
end: 313
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2196288
display id: annotation2196288
version: 1
top level: BBa_K851002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K851002/annotation2196288/range2196288/1

https://synbiohub.org/public/igem/BBa_K851002/annotation2196288/range2196288/1
type: Range
persistent identity: range2196288
display id: range2196288
version: 1
direction: direction/forward
top level: BBa_K851002
start: 271
end: 276
orientation: inline

XylR Binding Sites
type: SequenceAnnotation
persistent identity: annotation2196301
display id: annotation2196301
version: 1
top level: BBa_K851002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K851002/annotation2196301/range2196301/1

https://synbiohub.org/public/igem/BBa_K851002/annotation2196301/range2196301/1
type: Range
persistent identity: range2196301
display id: range2196301
version: 1
direction: direction/forward
top level: BBa_K851002
start: 288
end: 299
orientation: inline

pHS
type: ComponentDefinition
persistent identity: BBa K606040
display id: BBa_K606040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Hyperspank B.subtilis & E.coli
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter hyper-spank is an inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG).
mutable notes: DNA synthesis
mutable provenance: inspired from the part BBa_K143015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacO
sequence annotation: -35
sequence annotation: -10
sequence annotation: Lac0
sequence: https://synbiohub.org/public/igem/BBa_K606040_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation2138639
display id: annotation2138639
version: 1
top level: pHS
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K606040/annotation2138639/range2138639/1

https://synbiohub.org/public/igem/BBa_K606040/annotation2138639/range2138639/1
type: Range
persistent identity: range2138639
display id: range2138639
version: 1
direction: direction/forward
top level: pHS
start: 72
end: 92
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2138637
display id: annotation2138637
version: 1
top level: pHS
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K606040/annotation2138637/range2138637/1

https://synbiohub.org/public/igem/BBa_K606040/annotation2138637/range2138637/1
type: Range
persistent identity: range2138637
display id: range2138637
version: 1
direction: direction/forward
top level: pHS
start: 37
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2138638
display id: annotation2138638
version: 1
top level: pHS
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K606040/annotation2138638/range2138638/1

https://synbiohub.org/public/igem/BBa_K606040/annotation2138638/range2138638/1
type: Range
persistent identity: range2138638
display id: range2138638
version: 1
direction: direction/forward
top level: pHS
start: 60
end: 65
orientation: inline

Lac0
type: SequenceAnnotation
persistent identity: annotation2138636
display id: annotation2138636
version: 1
top level: pHS
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K606040/annotation2138636/range2138636/1

https://synbiohub.org/public/igem/BBa_K606040/annotation2138636/range2138636/1
type: Range
persistent identity: range2138636
display id: range2138636
version: 1
direction: direction/forward
top level: pHS
start: 1
end: 21
orientation: inline

amilCP
type: ComponentDefinition
persistent identity: BBa K592009
display id: BBa_K592009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP, blue chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
mutable notes: Illegal internal restriction site had to be removed (EcoRI).
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amilCP
sequence: https://synbiohub.org/public/igem/BBa_K592009_sequence/1

amilCP
type: SequenceAnnotation
persistent identity: annotation2131628
display id: annotation2131628
version: 1
top level: amilCP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1

https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1
type: Range
persistent identity: range2131628
display id: range2131628
version: 1
direction: direction/forward
top level: amilCP
start: 1
end: 666
orientation: inline

BBa_K608008
type: ComponentDefinition
persistent identity: BBa K608008
display id: BBa_K608008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive strong promoter with medium RBS and GFP
creator: Sandra Wassner
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 10299
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong promotor from the constitutive promotor family combined with medium RBS for strong gene expression. To quantify the gene expression, GFP was tagged to the promotor RBS domain.
mutable notes: We made this composite to test the effiency of the promotor and RBS domain.
mutable provenance: Composite from registry parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0032
component: BBa_E0040
component: BBa_J23104
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K608008_sequence/1

BBa_B0032
type: Component
persistent identity: component2127964
display id: component2127964
version: 1
top level: BBa_K608008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0040
type: Component
persistent identity: component2127967
display id: component2127967
version: 1
top level: BBa_K608008
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_J23104
type: Component
persistent identity: component2127962
display id: component2127962
version: 1
top level: BBa_K608008
access: public
definition: https://synbiohub.org/public/igem/BBa_J23104/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2127964
display id: annotation2127964
version: 1
top level: BBa_K608008
location: https://synbiohub.org/public/igem/BBa_K608008/annotation2127964/range2127964/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K608008/annotation2127964/range2127964/1
type: Range
persistent identity: range2127964
display id: range2127964
version: 1
direction: direction/forward
top level: BBa_K608008
start: 44
end: 56
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2127967
display id: annotation2127967
version: 1
top level: BBa_K608008
location: https://synbiohub.org/public/igem/BBa_K608008/annotation2127967/range2127967/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K608008/annotation2127967/range2127967/1
type: Range
persistent identity: range2127967
display id: range2127967
version: 1
direction: direction/reverse
top level: BBa_K608008
start: 63
end: 782
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2127962
display id: annotation2127962
version: 1
top level: BBa_K608008
location: https://synbiohub.org/public/igem/BBa_K608008/annotation2127962/range2127962/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608008/annotation2127962/range2127962/1
type: Range
persistent identity: range2127962
display id: range2127962
version: 1
direction: direction/reverse
top level: BBa_K608008
start: 1
end: 35
orientation: inline

ArgBox8
type: ComponentDefinition
persistent identity: BBa K778003
display id: BBa_K778003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ArgR binding sites （including 8 binding sites）
creator: Yuka Yamazaki
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Argbox tandem repeat 8
mutable notes: sukima
mutable provenance: synthesis(IDT)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Binding site 1 (argRp1)
sequence annotation: Binding site 4 (argIp)
sequence annotation: Binding site 2 (argRp1)
sequence annotation: Binding site 3 (argIp)
sequence: https://synbiohub.org/public/igem/BBa_K778003_sequence/1

Binding site 1 (argRp1)
type: SequenceAnnotation
persistent identity: annotation2201221
display id: annotation2201221
version: 1
top level: ArgBox8
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778003/annotation2201221/range2201221/1

https://synbiohub.org/public/igem/BBa_K778003/annotation2201221/range2201221/1
type: Range
persistent identity: range2201221
display id: range2201221
version: 1
direction: direction/forward
top level: ArgBox8
start: 1
end: 18
orientation: inline

Binding site 4 (argIp)
type: SequenceAnnotation
persistent identity: annotation2201231
display id: annotation2201231
version: 1
top level: ArgBox8
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778003/annotation2201231/range2201231/1

https://synbiohub.org/public/igem/BBa_K778003/annotation2201231/range2201231/1
type: Range
persistent identity: range2201231
display id: range2201231
version: 1
direction: direction/forward
top level: ArgBox8
start: 91
end: 108
orientation: inline

Binding site 2 (argRp1)
type: SequenceAnnotation
persistent identity: annotation2201246
display id: annotation2201246
version: 1
top level: ArgBox8
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778003/annotation2201246/range2201246/1

https://synbiohub.org/public/igem/BBa_K778003/annotation2201246/range2201246/1
type: Range
persistent identity: range2201246
display id: range2201246
version: 1
direction: direction/forward
top level: ArgBox8
start: 73
end: 90
orientation: inline

Binding site 4 (argIp)
type: SequenceAnnotation
persistent identity: annotation2201225
display id: annotation2201225
version: 1
top level: ArgBox8
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778003/annotation2201225/range2201225/1

https://synbiohub.org/public/igem/BBa_K778003/annotation2201225/range2201225/1
type: Range
persistent identity: range2201225
display id: range2201225
version: 1
direction: direction/forward
top level: ArgBox8
start: 55
end: 72
orientation: inline

Binding site 3 (argIp)
type: SequenceAnnotation
persistent identity: annotation2201222
display id: annotation2201222
version: 1
top level: ArgBox8
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778003/annotation2201222/range2201222/1

https://synbiohub.org/public/igem/BBa_K778003/annotation2201222/range2201222/1
type: Range
persistent identity: range2201222
display id: range2201222
version: 1
direction: direction/forward
top level: ArgBox8
start: 37
end: 54
orientation: inline

Binding site 3 (argIp)
type: SequenceAnnotation
persistent identity: annotation2201229
display id: annotation2201229
version: 1
top level: ArgBox8
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778003/annotation2201229/range2201229/1

https://synbiohub.org/public/igem/BBa_K778003/annotation2201229/range2201229/1
type: Range
persistent identity: range2201229
display id: range2201229
version: 1
direction: direction/forward
top level: ArgBox8
start: 109
end: 124
orientation: inline

Binding site 1 (argRp1)
type: SequenceAnnotation
persistent identity: annotation2201226
display id: annotation2201226
version: 1
top level: ArgBox8
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778003/annotation2201226/range2201226/1

https://synbiohub.org/public/igem/BBa_K778003/annotation2201226/range2201226/1
type: Range
persistent identity: range2201226
display id: range2201226
version: 1
direction: direction/forward
top level: ArgBox8
start: 125
end: 144
orientation: inline

Binding site 2 (argRp1)
type: SequenceAnnotation
persistent identity: annotation2200085
display id: annotation2200085
version: 1
top level: ArgBox8
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K778003/annotation2200085/range2200085/1

https://synbiohub.org/public/igem/BBa_K778003/annotation2200085/range2200085/1
type: Range
persistent identity: range2200085
display id: range2200085
version: 1
direction: direction/forward
top level: ArgBox8
start: 19
end: 36
orientation: inline

BBa_K817024
type: ComponentDefinition
persistent identity: BBa K817024
display id: BBa_K817024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-SP1-Penetratin-Flag-xx
creator: Jui-Tsen Yu
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1075_
m user id: 0
owner id: 13572
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: SP1-xx
mutable notes: SP1-xx
mutable provenance: SP1-xx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K817021
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K817021
sequence: https://synbiohub.org/public/igem/BBa_K817024_sequence/1

BBa_K817021
type: Component
persistent identity: component2201570
display id: component2201570
version: 1
top level: BBa_K817024
access: public
definition: BBa_K817021

BBa_B0015
type: Component
persistent identity: component2201577
display id: component2201577
version: 1
top level: BBa_K817024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2201577
display id: annotation2201577
version: 1
top level: BBa_K817024
location: https://synbiohub.org/public/igem/BBa_K817024/annotation2201577/range2201577/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K817024/annotation2201577/range2201577/1
type: Range
persistent identity: range2201577
display id: range2201577
version: 1
direction: direction/reverse
top level: BBa_K817024
start: 171
end: 299
orientation: inline

BBa_K817021
type: SequenceAnnotation
persistent identity: annotation2201570
display id: annotation2201570
version: 1
top level: BBa_K817024
location: https://synbiohub.org/public/igem/BBa_K817024/annotation2201570/range2201570/1
component: BBa_K817021

https://synbiohub.org/public/igem/BBa_K817024/annotation2201570/range2201570/1
type: Range
persistent identity: range2201570
display id: range2201570
version: 1
direction: direction/reverse
top level: BBa_K817024
start: 1
end: 162
orientation: inline

BBa_K539622
type: ComponentDefinition
persistent identity: BBa K539622
display id: BBa_K539622
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a RBS (B0032)+ilvC
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0032
component: BBa_K539711
sequence annotation: BBa_K539711
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K539622_sequence/1

BBa_B0032
type: Component
persistent identity: component2144135
display id: component2144135
version: 1
top level: BBa_K539622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K539711
type: Component
persistent identity: component2144138
display id: component2144138
version: 1
top level: BBa_K539622
access: public
definition: https://synbiohub.org/public/igem/BBa_K539711/1

BBa_K539711
type: SequenceAnnotation
persistent identity: annotation2144138
display id: annotation2144138
version: 1
top level: BBa_K539622
location: https://synbiohub.org/public/igem/BBa_K539622/annotation2144138/range2144138/1
component: BBa_K539711

https://synbiohub.org/public/igem/BBa_K539622/annotation2144138/range2144138/1
type: Range
persistent identity: range2144138
display id: range2144138
version: 1
direction: direction/reverse
top level: BBa_K539622
start: 20
end: 1495
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2144135
display id: annotation2144135
version: 1
top level: BBa_K539622
location: https://synbiohub.org/public/igem/BBa_K539622/annotation2144135/range2144135/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K539622/annotation2144135/range2144135/1
type: Range
persistent identity: range2144135
display id: range2144135
version: 1
direction: direction/forward
top level: BBa_K539622
start: 1
end: 13
orientation: inline

BBa_K554002
type: ComponentDefinition
persistent identity: BBa K554002
display id: BBa_K554002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HlyA secretion signal peptide
creator: UNICAMP EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: HylA is ...
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop
sequence annotation: HlyA
sequence: https://synbiohub.org/public/igem/BBa_K554002_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2136841
display id: annotation2136841
version: 1
top level: BBa_K554002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K554002/annotation2136841/range2136841/1

https://synbiohub.org/public/igem/BBa_K554002/annotation2136841/range2136841/1
type: Range
persistent identity: range2136841
display id: range2136841
version: 1
direction: direction/forward
top level: BBa_K554002
start: 181
end: 186
orientation: inline

HlyA
type: SequenceAnnotation
persistent identity: annotation2136840
display id: annotation2136840
version: 1
top level: BBa_K554002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K554002/annotation2136840/range2136840/1

https://synbiohub.org/public/igem/BBa_K554002/annotation2136840/range2136840/1
type: Range
persistent identity: range2136840
display id: range2136840
version: 1
direction: direction/forward
top level: BBa_K554002
start: 1
end: 180
orientation: inline

BBa_K592018
type: ComponentDefinition
persistent identity: BBa K592018
display id: BBa_K592018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-cph8
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the red light sensor Cph8 with RBS.
mutable notes: Since Cph8 was considerably longer than the RBS added, sequencing was necessary to confirm the construct.
mutable provenance: The cph8 was taken from Tabor's pJT122 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592000
component: BBa_B0034
sequence annotation: BBa_K592000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K592018_sequence/1

BBa_K592000
type: Component
persistent identity: component2136368
display id: component2136368
version: 1
top level: BBa_K592018
access: public
definition: https://synbiohub.org/public/igem/BBa_K592000/1

BBa_B0034
type: Component
persistent identity: component2136366
display id: component2136366
version: 1
top level: BBa_K592018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592000
type: SequenceAnnotation
persistent identity: annotation2136368
display id: annotation2136368
version: 1
top level: BBa_K592018
location: https://synbiohub.org/public/igem/BBa_K592018/annotation2136368/range2136368/1
component: BBa_K592000

https://synbiohub.org/public/igem/BBa_K592018/annotation2136368/range2136368/1
type: Range
persistent identity: range2136368
display id: range2136368
version: 1
direction: direction/reverse
top level: BBa_K592018
start: 19
end: 2256
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136366
display id: annotation2136366
version: 1
top level: BBa_K592018
location: https://synbiohub.org/public/igem/BBa_K592018/annotation2136366/range2136366/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592018/annotation2136366/range2136366/1
type: Range
persistent identity: range2136366
display id: range2136366
version: 1
direction: direction/reverse
top level: BBa_K592018
start: 1
end: 12
orientation: inline

MB-CFP
type: ComponentDefinition
persistent identity: BBa K774006
display id: BBa_K774006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420
creator: NRP-UEA-Norwich
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1026_
m user id: 0
owner id: 12400
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Our hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , in the orientation mammalian to bacterial, both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably. The hybrid promoter has been attached to the reporter: enhanced Cyan Fluorescence Protein (eCFP). The hybrid promoter has been characterised by observing expression of flourescent protein, and found to have increased transcription in response to increasing concentrations of potassium nitrate.
mutable notes: This biobrick contains the RBS and terminators, meaning we can simply add the promoter of choice to complete the system.
mutable provenance: E9-ns2 CArG promoter sequence + BHN + BBaK216005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K774001
component: BBa_E0420
sequence annotation: BBa_E0420
sequence annotation: BBa_K774001
sequence: https://synbiohub.org/public/igem/BBa_K774006_sequence/1

BBa_K774001
type: Component
persistent identity: component2187180
display id: component2187180
version: 1
top level: MB-CFP
access: public
definition: M-B

BBa_E0420
type: Component
persistent identity: component2187190
display id: component2187190
version: 1
top level: MB-CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0420/1

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2187190
display id: annotation2187190
version: 1
top level: MB-CFP
location: https://synbiohub.org/public/igem/BBa_K774006/annotation2187190/range2187190/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_K774006/annotation2187190/range2187190/1
type: Range
persistent identity: range2187190
display id: range2187190
version: 1
direction: direction/reverse
top level: MB-CFP
start: 217
end: 1094
orientation: inline

BBa_K774001
type: SequenceAnnotation
persistent identity: annotation2187180
display id: annotation2187180
version: 1
top level: MB-CFP
location: https://synbiohub.org/public/igem/BBa_K774006/annotation2187180/range2187180/1
component: BBa_K774001

https://synbiohub.org/public/igem/BBa_K774006/annotation2187180/range2187180/1
type: Range
persistent identity: range2187180
display id: range2187180
version: 1
direction: direction/reverse
top level: MB-CFP
start: 1
end: 208
orientation: inline

Tse2/Tsi2
type: ComponentDefinition
persistent identity: BBa K314202
display id: BBa_K314202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Toxin+ Antitoxin-Tse2 + Tsi2
creator: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Tse2/Tsi2, toxin antitoxin system. Tse2: A toxic protein originating from pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate p. aruginosas type 6 secretion system. Tsi2: Protein originating from pseudomonas aeruginosa that confers immunity to the toxic protein Tse2.
mutable provenance: pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tsi2
sequence annotation: tse2
sequence: https://synbiohub.org/public/igem/BBa_K314202_sequence/1

tsi2
type: SequenceAnnotation
persistent identity: annotation2089459
display id: annotation2089459
version: 1
top level: Tse2/Tsi2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K314202/annotation2089459/range2089459/1

https://synbiohub.org/public/igem/BBa_K314202/annotation2089459/range2089459/1
type: Range
persistent identity: range2089459
display id: range2089459
version: 1
direction: direction/forward
top level: Tse2/Tsi2
start: 487
end: 720
orientation: inline

tse2
type: SequenceAnnotation
persistent identity: annotation2089458
display id: annotation2089458
version: 1
top level: Tse2/Tsi2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K314202/annotation2089458/range2089458/1

https://synbiohub.org/public/igem/BBa_K314202/annotation2089458/range2089458/1
type: Range
persistent identity: range2089458
display id: range2089458
version: 1
direction: direction/forward
top level: Tse2/Tsi2
start: 1
end: 477
orientation: inline

Lov-tetR
type: ComponentDefinition
persistent identity: BBa K785000
display id: BBa_K785000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov-tetR (light sensor with Ptet repression) light->PtetO repressed
creator: Zining Hou
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1040_
m user id: 0
owner id: 11000
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light. This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA. We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.
mutable notes: This part is designed for our biowave project.
mutable provenance: This coding sequence is form the Avena sativa phototrophin1(AsLOV2) and tetracycline resistance. The whole gene is synthesized by Invitrogen company.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker part
sequence annotation: tetR DNA binding domain
sequence annotation: Lov light sensor domain
sequence: https://synbiohub.org/public/igem/BBa_K785000_sequence/1

linker part
type: SequenceAnnotation
persistent identity: annotation2194042
display id: annotation2194042
version: 1
top level: Lov-tetR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194042/range2194042/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194042/range2194042/1
type: Range
persistent identity: range2194042
display id: range2194042
version: 1
direction: direction/forward
top level: Lov-tetR
start: 213
end: 345
orientation: inline

tetR DNA binding domain
type: SequenceAnnotation
persistent identity: annotation2194043
display id: annotation2194043
version: 1
top level: Lov-tetR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194043/range2194043/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194043/range2194043/1
type: Range
persistent identity: range2194043
display id: range2194043
version: 1
direction: direction/forward
top level: Lov-tetR
start: 294
end: 1044
orientation: inline

Lov light sensor domain
type: SequenceAnnotation
persistent identity: annotation2194041
display id: annotation2194041
version: 1
top level: Lov-tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194041/range2194041/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194041/range2194041/1
type: Range
persistent identity: range2194041
display id: range2194041
version: 1
direction: direction/forward
top level: Lov-tetR
start: 1
end: 294
orientation: inline

BBa_K608002
type: ComponentDefinition
persistent identity: BBa K608002
display id: BBa_K608002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong Promoter and strong RBS
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: you can insert your part behind this part so it will be immediatly expressed in e.coli.
mutable notes: cloning via 3a-assembly
mutable provenance: assembly from iGEM parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23104
component: BBa_B0034
sequence annotation: BBa_J23104
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K608002_sequence/1

BBa_J23104
type: Component
persistent identity: component2128646
display id: component2128646
version: 1
top level: BBa_K608002
access: public
definition: https://synbiohub.org/public/igem/BBa_J23104/1

BBa_B0034
type: Component
persistent identity: component2128648
display id: component2128648
version: 1
top level: BBa_K608002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128646
display id: annotation2128646
version: 1
top level: BBa_K608002
location: https://synbiohub.org/public/igem/BBa_K608002/annotation2128646/range2128646/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608002/annotation2128646/range2128646/1
type: Range
persistent identity: range2128646
display id: range2128646
version: 1
direction: direction/reverse
top level: BBa_K608002
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128648
display id: annotation2128648
version: 1
top level: BBa_K608002
location: https://synbiohub.org/public/igem/BBa_K608002/annotation2128648/range2128648/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K608002/annotation2128648/range2128648/1
type: Range
persistent identity: range2128648
display id: range2128648
version: 1
direction: direction/reverse
top level: BBa_K608002
start: 44
end: 55
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_R0010
component: BBa_E1010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_K733006
type: ComponentDefinition
persistent identity: BBa K733006
display id: BBa_K733006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ydjM+Bmp2
creator: Chris Yu Lai Cheong; LI Yiming
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mature BMP-2
sequence annotation: Signal Peptide, YdjM
sequence: https://synbiohub.org/public/igem/BBa_K733006_sequence/1

Mature BMP-2
type: SequenceAnnotation
persistent identity: annotation2193746
display id: annotation2193746
version: 1
top level: BBa_K733006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K733006/annotation2193746/range2193746/1

https://synbiohub.org/public/igem/BBa_K733006/annotation2193746/range2193746/1
type: Range
persistent identity: range2193746
display id: range2193746
version: 1
direction: direction/forward
top level: BBa_K733006
start: 114
end: 461
orientation: inline

Signal Peptide, YdjM
type: SequenceAnnotation
persistent identity: annotation2193745
display id: annotation2193745
version: 1
top level: BBa_K733006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K733006/annotation2193745/range2193745/1

https://synbiohub.org/public/igem/BBa_K733006/annotation2193745/range2193745/1
type: Range
persistent identity: range2193745
display id: range2193745
version: 1
direction: direction/forward
top level: BBa_K733006
start: 1
end: 113
orientation: inline

BBa_K575040
type: ComponentDefinition
persistent identity: BBa K575040
display id: BBa_K575040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Genomic promoter with GFP reporter
mutable notes: no
mutable provenance: Genome and registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0034
component: BBa_K575020
sequence annotation: BBa_B0034
sequence annotation: BBa_K575020
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K575040_sequence/1

BBa_E0040
type: Component
persistent identity: component2138504
display id: component2138504
version: 1
top level: BBa_K575040
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component2138502
display id: component2138502
version: 1
top level: BBa_K575040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K575020
type: Component
persistent identity: component2138500
display id: component2138500
version: 1
top level: BBa_K575040
access: public
definition: BBa_K575020

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2138502
display id: annotation2138502
version: 1
top level: BBa_K575040
location: https://synbiohub.org/public/igem/BBa_K575040/annotation2138502/range2138502/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K575040/annotation2138502/range2138502/1
type: Range
persistent identity: range2138502
display id: range2138502
version: 1
direction: direction/reverse
top level: BBa_K575040
start: 810
end: 821
orientation: inline

BBa_K575020
type: SequenceAnnotation
persistent identity: annotation2138500
display id: annotation2138500
version: 1
top level: BBa_K575040
location: https://synbiohub.org/public/igem/BBa_K575040/annotation2138500/range2138500/1
component: BBa_K575020

https://synbiohub.org/public/igem/BBa_K575040/annotation2138500/range2138500/1
type: Range
persistent identity: range2138500
display id: range2138500
version: 1
direction: direction/reverse
top level: BBa_K575040
start: 1
end: 801
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2138504
display id: annotation2138504
version: 1
top level: BBa_K575040
location: https://synbiohub.org/public/igem/BBa_K575040/annotation2138504/range2138504/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K575040/annotation2138504/range2138504/1
type: Range
persistent identity: range2138504
display id: range2138504
version: 1
direction: direction/reverse
top level: BBa_K575040
start: 828
end: 1547
orientation: inline

MB-RFP
type: ComponentDefinition
persistent identity: BBa K774007
display id: BBa_K774007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mammalian-Bacterial promoter with RFP reporter: CArG promoter sequence + BBaK216005 + BBa_K08101
creator: NRP-UEA-Norwich
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1026_
m user id: 0
owner id: 12400
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Our hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , in the orientation mammalian to bacterial, both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably. The hybrid promoter has been attached to the reporter: Red Fluorescent Protein (RFP). The hybrid promoter has been characterised by observing expression of flourescent protein, and found to have increased transcription in response to increasing concentrations of potassium nitrate.
mutable notes: -
mutable provenance: E9-ns2 CArG promoter sequence + BHN + BBaK216005 + BBa_K081014
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K081014
component: BBa_K774001
sequence annotation: BBa_K081014
sequence annotation: BBa_K774001
sequence: https://synbiohub.org/public/igem/BBa_K774007_sequence/1

BBa_K081014
type: Component
persistent identity: component2251086
display id: component2251086
version: 1
top level: MB-RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K081014/1

BBa_K774001
type: Component
persistent identity: component2251074
display id: component2251074
version: 1
top level: MB-RFP
access: public
definition: M-B

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2251086
display id: annotation2251086
version: 1
top level: MB-RFP
location: https://synbiohub.org/public/igem/BBa_K774007/annotation2251086/range2251086/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K774007/annotation2251086/range2251086/1
type: Range
persistent identity: range2251086
display id: range2251086
version: 1
direction: direction/reverse
top level: MB-RFP
start: 217
end: 990
orientation: inline

BBa_K774001
type: SequenceAnnotation
persistent identity: annotation2251074
display id: annotation2251074
version: 1
top level: MB-RFP
location: https://synbiohub.org/public/igem/BBa_K774007/annotation2251074/range2251074/1
component: BBa_K774001

https://synbiohub.org/public/igem/BBa_K774007/annotation2251074/range2251074/1
type: Range
persistent identity: range2251074
display id: range2251074
version: 1
direction: direction/reverse
top level: MB-RFP
start: 1
end: 208
orientation: inline

BBa_K801039
type: ComponentDefinition
persistent identity: BBa K801039
display id: BBa_K801039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CDS for a synthetic hybrid fusion protein. Consists of serveral domains. RFC10 pre and suffix
mutable notes: -
mutable provenance: CDS for a synthetic hybrid fusion protein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HA epitope tag
sequence annotation: T7 promoter (for sequencing)
sequence annotation: GAL4 activation domain (GAL4AD)
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: EcoRI/MfeI fusion scar
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K801039_sequence/1

HA epitope tag
type: SequenceAnnotation
persistent identity: annotation2200012
display id: annotation2200012
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1
type: Range
persistent identity: range2200012
display id: range2200012
version: 1
direction: direction/forward
top level: BBa_K801039
start: 457
end: 477
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2200010
display id: annotation2200010
version: 1
top level: BBa_K801039
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1
type: Range
persistent identity: range2200010
display id: range2200010
version: 1
direction: direction/forward
top level: BBa_K801039
start: 414
end: 436
orientation: inline

GAL4 activation domain (GAL4AD)
type: SequenceAnnotation
persistent identity: annotation2199997
display id: annotation2199997
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1
type: Range
persistent identity: range2199997
display id: range2199997
version: 1
direction: direction/forward
top level: BBa_K801039
start: 70
end: 408
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199929
display id: annotation2199929
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1
type: Range
persistent identity: range2199929
display id: range2199929
version: 1
direction: direction/forward
top level: BBa_K801039
start: 31
end: 51
orientation: inline

EcoRI/MfeI fusion scar
type: SequenceAnnotation
persistent identity: annotation2200000
display id: annotation2200000
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1
type: Range
persistent identity: range2200000
display id: range2200000
version: 1
direction: direction/forward
top level: BBa_K801039
start: 499
end: 504
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2199998
display id: annotation2199998
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1
type: Range
persistent identity: range2199998
display id: range2199998
version: 1
direction: direction/forward
top level: BBa_K801039
start: 503
end: 801
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2200008
display id: annotation2200008
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1
type: Range
persistent identity: range2200008
display id: range2200008
version: 1
direction: direction/forward
top level: BBa_K801039
start: 409
end: 498
orientation: inline

1C2
type: ComponentDefinition
persistent identity: BBa K590054
display id: BBa_K590054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RrD_N25M-PSB1C3-High constitutive
creator: Lei Zheng, Ben Mo, Elaine Lai, Juhye An
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Plasmid that consists of Acyl-ACP Reductase, rbs, mutant Synechococcus elongatus PCC7942 Aldehyde Decarbonylase in PSB1C3 high consititutive vector for alkane production
mutable notes: TBD
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K590037
component: BBa_K314100
component: BBa_K590032
sequence annotation: BBa_K590032
sequence annotation: BBa_K314100
sequence annotation: BBa_K590037
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K590054_sequence/1

BBa_B0034
type: Component
persistent identity: component2129338
display id: component2129338
version: 1
top level: 1C2
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K590037
type: Component
persistent identity: component2129339
display id: component2129339
version: 1
top level: 1C2
access: public
definition: https://synbiohub.org/public/igem/BBa_K590037/1

BBa_K314100
type: Component
persistent identity: component2129335
display id: component2129335
version: 1
top level: 1C2
access: public
definition: BBa_K314100

BBa_K590032
type: Component
persistent identity: component2129336
display id: component2129336
version: 1
top level: 1C2
access: public
definition: https://synbiohub.org/public/igem/BBa_K590032/1

BBa_K590032
type: SequenceAnnotation
persistent identity: annotation2129336
display id: annotation2129336
version: 1
top level: 1C2
location: https://synbiohub.org/public/igem/BBa_K590054/annotation2129336/range2129336/1
component: BBa_K590032

https://synbiohub.org/public/igem/BBa_K590054/annotation2129336/range2129336/1
type: Range
persistent identity: range2129336
display id: range2129336
version: 1
direction: direction/reverse
top level: 1C2
start: 525
end: 1610
orientation: inline

BBa_K314100
type: SequenceAnnotation
persistent identity: annotation2129335
display id: annotation2129335
version: 1
top level: 1C2
location: https://synbiohub.org/public/igem/BBa_K590054/annotation2129335/range2129335/1
component: BBa_K314100

https://synbiohub.org/public/igem/BBa_K590054/annotation2129335/range2129335/1
type: Range
persistent identity: range2129335
display id: range2129335
version: 1
direction: direction/reverse
top level: 1C2
start: 1
end: 518
orientation: inline

BBa_K590037
type: SequenceAnnotation
persistent identity: annotation2129339
display id: annotation2129339
version: 1
top level: 1C2
location: https://synbiohub.org/public/igem/BBa_K590054/annotation2129339/range2129339/1
component: BBa_K590037

https://synbiohub.org/public/igem/BBa_K590054/annotation2129339/range2129339/1
type: Range
persistent identity: range2129339
display id: range2129339
version: 1
direction: direction/reverse
top level: 1C2
start: 1637
end: 2392
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2129338
display id: annotation2129338
version: 1
top level: 1C2
location: https://synbiohub.org/public/igem/BBa_K590054/annotation2129338/range2129338/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K590054/annotation2129338/range2129338/1
type: Range
persistent identity: range2129338
display id: range2129338
version: 1
direction: direction/reverse
top level: 1C2
start: 1619
end: 1630
orientation: inline

BBa_K611026
type: ComponentDefinition
persistent identity: BBa K611026
display id: BBa_K611026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Promoter Variant #6
creator: Nicholas Csicsery
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.
mutable notes: We chose to mutate this part from a well known promoter so that any changes in behavior would be easy to observe.
mutable provenance: Mutagenic PCR of BBa_R0010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: LacI binding site
sequence annotation: -10
sequence annotation: t->c
sequence annotation: -35
sequence annotation: CAP binding site
sequence annotation: end of LacI coding region (inactive)
sequence annotation: c->t
sequence: https://synbiohub.org/public/igem/BBa_K611026_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2136220
display id: annotation2136220
version: 1
top level: BBa_K611026
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K611026/annotation2136220/range2136220/1

https://synbiohub.org/public/igem/BBa_K611026/annotation2136220/range2136220/1
type: Range
persistent identity: range2136220
display id: range2136220
version: 1
direction: direction/forward
top level: BBa_K611026
start: 173
end: 173
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2134964
display id: annotation2134964
version: 1
top level: BBa_K611026
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K611026/annotation2134964/range2134964/1

https://synbiohub.org/public/igem/BBa_K611026/annotation2134964/range2134964/1
type: Range
persistent identity: range2134964
display id: range2134964
version: 1
direction: direction/forward
top level: BBa_K611026
start: 166
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2136219
display id: annotation2136219
version: 1
top level: BBa_K611026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K611026/annotation2136219/range2136219/1

https://synbiohub.org/public/igem/BBa_K611026/annotation2136219/range2136219/1
type: Range
persistent identity: range2136219
display id: range2136219
version: 1
direction: direction/forward
top level: BBa_K611026
start: 161
end: 166
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation2134962
display id: annotation2134962
version: 1
top level: BBa_K611026
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K611026/annotation2134962/range2134962/1

https://synbiohub.org/public/igem/BBa_K611026/annotation2134962/range2134962/1
type: Range
persistent identity: range2134962
display id: range2134962
version: 1
direction: direction/forward
top level: BBa_K611026
start: 138
end: 138
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2136218
display id: annotation2136218
version: 1
top level: BBa_K611026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K611026/annotation2136218/range2136218/1

https://synbiohub.org/public/igem/BBa_K611026/annotation2136218/range2136218/1
type: Range
persistent identity: range2136218
display id: range2136218
version: 1
direction: direction/forward
top level: BBa_K611026
start: 137
end: 142
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2136217
display id: annotation2136217
version: 1
top level: BBa_K611026
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K611026/annotation2136217/range2136217/1

https://synbiohub.org/public/igem/BBa_K611026/annotation2136217/range2136217/1
type: Range
persistent identity: range2136217
display id: range2136217
version: 1
direction: direction/forward
top level: BBa_K611026
start: 89
end: 126
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2136216
display id: annotation2136216
version: 1
top level: BBa_K611026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K611026/annotation2136216/range2136216/1

https://synbiohub.org/public/igem/BBa_K611026/annotation2136216/range2136216/1
type: Range
persistent identity: range2136216
display id: range2136216
version: 1
direction: direction/forward
top level: BBa_K611026
start: 1
end: 88
orientation: inline

c->t
type: SequenceAnnotation
persistent identity: annotation2134963
display id: annotation2134963
version: 1
top level: BBa_K611026
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K611026/annotation2134963/range2134963/1

https://synbiohub.org/public/igem/BBa_K611026/annotation2134963/range2134963/1
type: Range
persistent identity: range2134963
display id: range2134963
version: 1
direction: direction/forward
top level: BBa_K611026
start: 159
end: 159
orientation: inline

BBa_K823042
type: ComponentDefinition
persistent identity: BBa K823042
display id: BBa_K823042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PsspK promoter regulated by sigma G
creator: Julia Bartels
created: 2012-09-09T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
group u list: _1081_
m user id: 0
owner id: 9472
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A B.subtilis promoter regulated by sigma G late in Sporulation in the forespore
mutable notes: none
mutable provenance: B.subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PsspK
sequence: https://synbiohub.org/public/igem/BBa_K823042_sequence/1

PsspK
type: SequenceAnnotation
persistent identity: annotation2182372
display id: annotation2182372
version: 1
top level: BBa_K823042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823042/annotation2182372/range2182372/1

https://synbiohub.org/public/igem/BBa_K823042/annotation2182372/range2182372/1
type: Range
persistent identity: range2182372
display id: range2182372
version: 1
direction: direction/forward
top level: BBa_K823042
start: 1
end: 94
orientation: inline

BBa_K823009
type: ComponentDefinition
persistent identity: BBa K823009
display id: BBa_K823009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23107
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23107 (with one nucleotide different to the original Anderson promoter sequence) without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23107
sequence: https://synbiohub.org/public/igem/BBa_K823009_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182532
display id: annotation2182532
version: 1
top level: BBa_K823009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823009/annotation2182532/range2182532/1

https://synbiohub.org/public/igem/BBa_K823009/annotation2182532/range2182532/1
type: Range
persistent identity: range2182532
display id: range2182532
version: 1
direction: direction/forward
top level: BBa_K823009
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182533
display id: annotation2182533
version: 1
top level: BBa_K823009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823009/annotation2182533/range2182533/1

https://synbiohub.org/public/igem/BBa_K823009/annotation2182533/range2182533/1
type: Range
persistent identity: range2182533
display id: range2182533
version: 1
direction: direction/forward
top level: BBa_K823009
start: 24
end: 29
orientation: inline

J23107
type: SequenceAnnotation
persistent identity: annotation2182531
display id: annotation2182531
version: 1
top level: BBa_K823009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823009/annotation2182531/range2182531/1

https://synbiohub.org/public/igem/BBa_K823009/annotation2182531/range2182531/1
type: Range
persistent identity: range2182531
display id: range2182531
version: 1
direction: direction/forward
top level: BBa_K823009
start: 1
end: 35
orientation: inline

BBa_K875009
type: ComponentDefinition
persistent identity: BBa K875009
display id: BBa_K875009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LL 37 - Cathelicidin
creator: Giulia Corso, Sara Samari
created: 2012-09-22T11:00:00Z
modified: 2016-01-27T02:41:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1136_
m user id: 4206
owner id: 12657
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A new LL 37 bacteria toxin optimized for E. coli expression. The toxin disrupt the membrane from outside and is armless from inside.
mutable notes: by synthesis
mutable provenance: Ulrich et al., 2006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K875009_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2200494
display id: annotation2200494
version: 1
top level: BBa_K875009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K875009/annotation2200494/range2200494/1

https://synbiohub.org/public/igem/BBa_K875009/annotation2200494/range2200494/1
type: Range
persistent identity: range2200494
display id: range2200494
version: 1
direction: direction/forward
top level: BBa_K875009
start: 1
end: 3
orientation: inline

BBa_K608010
type: ComponentDefinition
persistent identity: BBa K608010
display id: BBa_K608010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium promoter with strong RBS and GFP
creator: Sandra Wassner
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 10299
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium promotor from the constitutive promotor family combined with a strong RBS and tagged with GFP to quantify the gene expression.
mutable notes: We made this composite to test the effiency of promotor and RBS.
mutable provenance: Composite from the registry parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23110
component: BBa_E0040
component: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K608010_sequence/1

BBa_J23110
type: Component
persistent identity: component2127991
display id: component2127991
version: 1
top level: BBa_K608010
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_E0040
type: Component
persistent identity: component2127995
display id: component2127995
version: 1
top level: BBa_K608010
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component2127993
display id: component2127993
version: 1
top level: BBa_K608010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2127991
display id: annotation2127991
version: 1
top level: BBa_K608010
location: https://synbiohub.org/public/igem/BBa_K608010/annotation2127991/range2127991/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K608010/annotation2127991/range2127991/1
type: Range
persistent identity: range2127991
display id: range2127991
version: 1
direction: direction/reverse
top level: BBa_K608010
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2127993
display id: annotation2127993
version: 1
top level: BBa_K608010
location: https://synbiohub.org/public/igem/BBa_K608010/annotation2127993/range2127993/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K608010/annotation2127993/range2127993/1
type: Range
persistent identity: range2127993
display id: range2127993
version: 1
direction: direction/reverse
top level: BBa_K608010
start: 44
end: 55
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2127995
display id: annotation2127995
version: 1
top level: BBa_K608010
location: https://synbiohub.org/public/igem/BBa_K608010/annotation2127995/range2127995/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K608010/annotation2127995/range2127995/1
type: Range
persistent identity: range2127995
display id: range2127995
version: 1
direction: direction/reverse
top level: BBa_K608010
start: 62
end: 781
orientation: inline

BBa_K654058
type: ComponentDefinition
persistent identity: BBa K654058
display id: BBa_K654058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Thioesterase (TesA from E. coli) with 8-His Tag
creator: Cody Tramp
created: 2011-08-11T11:00:00Z
modified: 2015-05-08T01:13:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _831_
m user id: 0
owner id: 6788
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: TesA thioesterase gene from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction.
mutable notes: 8-His tag added just before the stop codon - C terminus end of the protein. May alter activity level.
mutable provenance: E. coli genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Stop
sequence annotation: 8-His Tag
sequence annotation: Thioesterase (TesA)
sequence: https://synbiohub.org/public/igem/BBa_K654058_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2124921
display id: annotation2124921
version: 1
top level: BBa_K654058
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K654058/annotation2124921/range2124921/1

https://synbiohub.org/public/igem/BBa_K654058/annotation2124921/range2124921/1
type: Range
persistent identity: range2124921
display id: range2124921
version: 1
direction: direction/forward
top level: BBa_K654058
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2124922
display id: annotation2124922
version: 1
top level: BBa_K654058
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K654058/annotation2124922/range2124922/1

https://synbiohub.org/public/igem/BBa_K654058/annotation2124922/range2124922/1
type: Range
persistent identity: range2124922
display id: range2124922
version: 1
direction: direction/forward
top level: BBa_K654058
start: 649
end: 651
orientation: inline

8-His Tag
type: SequenceAnnotation
persistent identity: annotation2124923
display id: annotation2124923
version: 1
top level: BBa_K654058
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K654058/annotation2124923/range2124923/1

https://synbiohub.org/public/igem/BBa_K654058/annotation2124923/range2124923/1
type: Range
persistent identity: range2124923
display id: range2124923
version: 1
direction: direction/forward
top level: BBa_K654058
start: 625
end: 648
orientation: inline

Thioesterase (TesA)
type: SequenceAnnotation
persistent identity: annotation2124924
display id: annotation2124924
version: 1
top level: BBa_K654058
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K654058/annotation2124924/range2124924/1

https://synbiohub.org/public/igem/BBa_K654058/annotation2124924/range2124924/1
type: Range
persistent identity: range2124924
display id: range2124924
version: 1
direction: direction/forward
top level: BBa_K654058
start: 1
end: 624
orientation: inline

BBa_K861172
type: ComponentDefinition
persistent identity: BBa K861172
display id: BBa_K861172
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI generator controlled by PcstA (glucose-repressible promoter)
creator: Xian Xia
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda cI is controlled by promoter PcstA,which can be activated by CRP-cAMP complex.Therefore protein lambda cI will be expressed under low glucose concentration.
mutable notes: None
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K118011
component: BBa_P0451
sequence annotation: BBa_K118011
sequence annotation: BBa_P0451
sequence: https://synbiohub.org/public/igem/BBa_K861172_sequence/1

BBa_K118011
type: Component
persistent identity: component2182076
display id: component2182076
version: 1
top level: BBa_K861172
access: public
definition: https://synbiohub.org/public/igem/BBa_K118011/1

BBa_P0451
type: Component
persistent identity: component2182089
display id: component2182089
version: 1
top level: BBa_K861172
access: public
definition: https://synbiohub.org/public/igem/BBa_P0451/1

BBa_K118011
type: SequenceAnnotation
persistent identity: annotation2182076
display id: annotation2182076
version: 1
top level: BBa_K861172
location: https://synbiohub.org/public/igem/BBa_K861172/annotation2182076/range2182076/1
component: BBa_K118011

https://synbiohub.org/public/igem/BBa_K861172/annotation2182076/range2182076/1
type: Range
persistent identity: range2182076
display id: range2182076
version: 1
direction: direction/reverse
top level: BBa_K861172
start: 1
end: 131
orientation: inline

BBa_P0451
type: SequenceAnnotation
persistent identity: annotation2182089
display id: annotation2182089
version: 1
top level: BBa_K861172
location: https://synbiohub.org/public/igem/BBa_K861172/annotation2182089/range2182089/1
component: BBa_P0451

https://synbiohub.org/public/igem/BBa_K861172/annotation2182089/range2182089/1
type: Range
persistent identity: range2182089
display id: range2182089
version: 1
direction: direction/reverse
top level: BBa_K861172
start: 140
end: 1069
orientation: inline

BBa_K523004
type: ComponentDefinition
persistent identity: BBa K523004
display id: BBa_K523004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bglX for BioSandwich
creator: Sylvia Ispasanie, Mun Ching Lee
created: 2011-07-28T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a version of BBa_K523002 but with no RBS and no stop codon. There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.
mutable notes: The part was made using the strategy outlined in BBa_K523000 , and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. The part was created from a lab ''E. coli'' strain using primers: * ACT AGATCT atg aaa tgg cta tgt tca gta gg ** Forward; adds BglII site; starts at start codon. * ACT ACTAGT ACC cag caa ctc aaa ctc gcc ttt c ** Reverse; adds SpeI site; BioSandwich compliant; no stop codons, adds a glycine.
mutable provenance: See BBa_K523002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BglII
sequence annotation: PstI site removed
sequence annotation: ATG
sequence annotation: GG
sequence annotation: bglX
sequence: https://synbiohub.org/public/igem/BBa_K523004_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation2124179
display id: annotation2124179
version: 1
top level: BBa_K523004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523004/annotation2124179/range2124179/1

https://synbiohub.org/public/igem/BBa_K523004/annotation2124179/range2124179/1
type: Range
persistent identity: range2124179
display id: range2124179
version: 1
direction: direction/forward
top level: BBa_K523004
start: 1
end: 4
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2125497
display id: annotation2125497
version: 1
top level: BBa_K523004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K523004/annotation2125497/range2125497/1

https://synbiohub.org/public/igem/BBa_K523004/annotation2125497/range2125497/1
type: Range
persistent identity: range2125497
display id: range2125497
version: 1
direction: direction/forward
top level: BBa_K523004
start: 52
end: 52
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2124177
display id: annotation2124177
version: 1
top level: BBa_K523004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K523004/annotation2124177/range2124177/1

https://synbiohub.org/public/igem/BBa_K523004/annotation2124177/range2124177/1
type: Range
persistent identity: range2124177
display id: range2124177
version: 1
direction: direction/forward
top level: BBa_K523004
start: 5
end: 5
orientation: inline

GG
type: SequenceAnnotation
persistent identity: annotation2124178
display id: annotation2124178
version: 1
top level: BBa_K523004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523004/annotation2124178/range2124178/1

https://synbiohub.org/public/igem/BBa_K523004/annotation2124178/range2124178/1
type: Range
persistent identity: range2124178
display id: range2124178
version: 1
direction: direction/forward
top level: BBa_K523004
start: 2300
end: 2301
orientation: inline

bglX
type: SequenceAnnotation
persistent identity: annotation2124176
display id: annotation2124176
version: 1
top level: BBa_K523004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523004/annotation2124176/range2124176/1

https://synbiohub.org/public/igem/BBa_K523004/annotation2124176/range2124176/1
type: Range
persistent identity: range2124176
display id: range2124176
version: 1
direction: direction/forward
top level: BBa_K523004
start: 5
end: 2299
orientation: inline

tctB_162aa
type: ComponentDefinition
persistent identity: BBa K808003
display id: BBa_K808003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tctB_162: small subunit B1 of the tripartite tricarboxylate transporter family
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12829
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tctB_162: small subunit B1 of the tripartite tricarboxylate transporter family
mutable notes: -
mutable provenance: Comamonas testosteroni KF1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808003_sequence/1

BBa_K598007
type: ComponentDefinition
persistent identity: BBa K598007
display id: BBa_K598007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015
creator: Siyang HAO
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8816
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is a theophylline responsive riboswitch containing an original ribosome binding site.
mutable notes: It can be used as a reporter of gene expression intensity.
mutable provenance: Using PCR to get it in vivo.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP E0040
sequence annotation: Terminator B0015
sequence annotation: Native RBS
sequence annotation: Theophylline responsive riboswitch Theo P1G1
sequence: https://synbiohub.org/public/igem/BBa_K598007_sequence/1

GFP E0040
type: SequenceAnnotation
persistent identity: annotation2152709
display id: annotation2152709
version: 1
top level: BBa_K598007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K598007/annotation2152709/range2152709/1

https://synbiohub.org/public/igem/BBa_K598007/annotation2152709/range2152709/1
type: Range
persistent identity: range2152709
display id: range2152709
version: 1
direction: direction/forward
top level: BBa_K598007
start: 88
end: 807
orientation: inline

Terminator B0015
type: SequenceAnnotation
persistent identity: annotation2152711
display id: annotation2152711
version: 1
top level: BBa_K598007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598007/annotation2152711/range2152711/1

https://synbiohub.org/public/igem/BBa_K598007/annotation2152711/range2152711/1
type: Range
persistent identity: range2152711
display id: range2152711
version: 1
direction: direction/forward
top level: BBa_K598007
start: 816
end: 944
orientation: inline

Native RBS
type: SequenceAnnotation
persistent identity: annotation2152710
display id: annotation2152710
version: 1
top level: BBa_K598007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598007/annotation2152710/range2152710/1

https://synbiohub.org/public/igem/BBa_K598007/annotation2152710/range2152710/1
type: Range
persistent identity: range2152710
display id: range2152710
version: 1
direction: direction/forward
top level: BBa_K598007
start: 75
end: 78
orientation: inline

Theophylline responsive riboswitch Theo P1G1
type: SequenceAnnotation
persistent identity: annotation2152708
display id: annotation2152708
version: 1
top level: BBa_K598007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598007/annotation2152708/range2152708/1

https://synbiohub.org/public/igem/BBa_K598007/annotation2152708/range2152708/1
type: Range
persistent identity: range2152708
display id: range2152708
version: 1
direction: direction/forward
top level: BBa_K598007
start: 1
end: 87
orientation: inline

LigA
type: ComponentDefinition
persistent identity: BBa K525710
display id: BBa_K525710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: LigA1 mit Promotor, RBS, Histag
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: RBS
sequence annotation: LigA
sequence annotation: His-tag
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K525710_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2161302
display id: annotation2161302
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1
type: Range
persistent identity: range2161302
display id: range2161302
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 36
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161300
display id: annotation2161300
version: 1
top level: LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1
type: Range
persistent identity: range2161300
display id: range2161300
version: 1
direction: direction/forward
top level: LigA
start: 21
end: 33
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161301
display id: annotation2161301
version: 1
top level: LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1
type: Range
persistent identity: range2161301
display id: range2161301
version: 1
direction: direction/forward
top level: LigA
start: 24
end: 27
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2161303
display id: annotation2161303
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1
type: Range
persistent identity: range2161303
display id: range2161303
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 2045
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2161304
display id: annotation2161304
version: 1
top level: LigA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1
type: Range
persistent identity: range2161304
display id: range2161304
version: 1
direction: direction/forward
top level: LigA
start: 2047
end: 2064
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161299
display id: annotation2161299
version: 1
top level: LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1
type: Range
persistent identity: range2161299
display id: range2161299
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 20
orientation: inline

BBa_K822000
type: ComponentDefinition
persistent identity: BBa K822000
display id: BBa_K822000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lldPRD operon promoter + RBS from E. coli
creator: Jarle Pahr
created: 2012-09-22T11:00:00Z
modified: 2015-09-14T06:29:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1080_
m user id: 26442
owner id: 11627
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: lldP is a promoter associated with the lldPRD operon of E. coli. The lldPRD operon encodes a lactate-dehydrogenase, and the lldP promoter has been shown to be induced by L-lactate. The activity of lldP may be repressed by chloramphenicol and nutrient-rich growth media.
mutable notes: N/A.
mutable provenance: E. coli K12 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: transcriptional start site
sequence annotation: lldR binding site (O2)
sequence annotation: -10
sequence annotation: RBS
sequence annotation: lldR binding site (O1)
sequence: https://synbiohub.org/public/igem/BBa_K822000_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2200502
display id: annotation2200502
version: 1
top level: BBa_K822000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K822000/annotation2200502/range2200502/1

https://synbiohub.org/public/igem/BBa_K822000/annotation2200502/range2200502/1
type: Range
persistent identity: range2200502
display id: range2200502
version: 1
direction: direction/forward
top level: BBa_K822000
start: 201
end: 206
orientation: inline

transcriptional start site
type: SequenceAnnotation
persistent identity: annotation2200507
display id: annotation2200507
version: 1
top level: BBa_K822000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K822000/annotation2200507/range2200507/1

https://synbiohub.org/public/igem/BBa_K822000/annotation2200507/range2200507/1
type: Range
persistent identity: range2200507
display id: range2200507
version: 1
direction: direction/forward
top level: BBa_K822000
start: 235
end: 235
orientation: inline

lldR binding site (O2)
type: SequenceAnnotation
persistent identity: annotation2200500
display id: annotation2200500
version: 1
top level: BBa_K822000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K822000/annotation2200500/range2200500/1

https://synbiohub.org/public/igem/BBa_K822000/annotation2200500/range2200500/1
type: Range
persistent identity: range2200500
display id: range2200500
version: 1
direction: direction/forward
top level: BBa_K822000
start: 256
end: 272
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2200503
display id: annotation2200503
version: 1
top level: BBa_K822000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K822000/annotation2200503/range2200503/1

https://synbiohub.org/public/igem/BBa_K822000/annotation2200503/range2200503/1
type: Range
persistent identity: range2200503
display id: range2200503
version: 1
direction: direction/forward
top level: BBa_K822000
start: 224
end: 229
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2200508
display id: annotation2200508
version: 1
top level: BBa_K822000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K822000/annotation2200508/range2200508/1

https://synbiohub.org/public/igem/BBa_K822000/annotation2200508/range2200508/1
type: Range
persistent identity: range2200508
display id: range2200508
version: 1
direction: direction/forward
top level: BBa_K822000
start: 333
end: 338
orientation: inline

lldR binding site (O1)
type: SequenceAnnotation
persistent identity: annotation2200499
display id: annotation2200499
version: 1
top level: BBa_K822000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K822000/annotation2200499/range2200499/1

https://synbiohub.org/public/igem/BBa_K822000/annotation2200499/range2200499/1
type: Range
persistent identity: range2200499
display id: range2200499
version: 1
direction: direction/forward
top level: BBa_K822000
start: 130
end: 146
orientation: inline

BBa_K611025
type: ComponentDefinition
persistent identity: BBa K611025
display id: BBa_K611025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Promoter Variant #5
creator: Nicholas Csicsery
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.
mutable notes: We chose to mutate this part from a well known promoter so that any changes in behavior would be easy to observe.
mutable provenance: Mutagenic PCR of BBa_R0010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: CAP binding site
sequence annotation: a->c
sequence annotation: end of LacI coding region (inactive)
sequence annotation: -10
sequence annotation: -35
sequence annotation: t->c
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K611025_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2136215
display id: annotation2136215
version: 1
top level: BBa_K611025
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2136215/range2136215/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2136215/range2136215/1
type: Range
persistent identity: range2136215
display id: range2136215
version: 1
direction: direction/forward
top level: BBa_K611025
start: 173
end: 173
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2136212
display id: annotation2136212
version: 1
top level: BBa_K611025
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2136212/range2136212/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2136212/range2136212/1
type: Range
persistent identity: range2136212
display id: range2136212
version: 1
direction: direction/forward
top level: BBa_K611025
start: 89
end: 126
orientation: inline

a->c
type: SequenceAnnotation
persistent identity: annotation2134900
display id: annotation2134900
version: 1
top level: BBa_K611025
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2134900/range2134900/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2134900/range2134900/1
type: Range
persistent identity: range2134900
display id: range2134900
version: 1
direction: direction/forward
top level: BBa_K611025
start: 117
end: 117
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2136211
display id: annotation2136211
version: 1
top level: BBa_K611025
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2136211/range2136211/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2136211/range2136211/1
type: Range
persistent identity: range2136211
display id: range2136211
version: 1
direction: direction/forward
top level: BBa_K611025
start: 1
end: 88
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2136214
display id: annotation2136214
version: 1
top level: BBa_K611025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2136214/range2136214/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2136214/range2136214/1
type: Range
persistent identity: range2136214
display id: range2136214
version: 1
direction: direction/forward
top level: BBa_K611025
start: 161
end: 166
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2136213
display id: annotation2136213
version: 1
top level: BBa_K611025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2136213/range2136213/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2136213/range2136213/1
type: Range
persistent identity: range2136213
display id: range2136213
version: 1
direction: direction/forward
top level: BBa_K611025
start: 137
end: 142
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation2134901
display id: annotation2134901
version: 1
top level: BBa_K611025
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2134901/range2134901/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2134901/range2134901/1
type: Range
persistent identity: range2134901
display id: range2134901
version: 1
direction: direction/forward
top level: BBa_K611025
start: 123
end: 123
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation2134899
display id: annotation2134899
version: 1
top level: BBa_K611025
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2134899/range2134899/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2134899/range2134899/1
type: Range
persistent identity: range2134899
display id: range2134899
version: 1
direction: direction/forward
top level: BBa_K611025
start: 30
end: 30
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2134902
display id: annotation2134902
version: 1
top level: BBa_K611025
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K611025/annotation2134902/range2134902/1

https://synbiohub.org/public/igem/BBa_K611025/annotation2134902/range2134902/1
type: Range
persistent identity: range2134902
display id: range2134902
version: 1
direction: direction/forward
top level: BBa_K611025
start: 166
end: 200
orientation: inline

pLac ColE2
type: ComponentDefinition
persistent identity: BBa K914001
display id: BBa_K914001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac Colicin E2 Immunity protein
creator: Julianne Rieders
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13463
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Colicin E2 Immunity protein under pLac promoter (BBa_R0011) with repressilator RBS. This part can be used to confer immunity against the colicin E2 activity protein by induction with IPTG.
mutable notes: We selected the repressilator RBS and pLac to drive production of Colicin E2 Immunity protein
mutable provenance: pDEW Colicin E2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: lac O1
sequence annotation: BBa_K914002
sequence annotation: -10
sequence annotation: repressilator
sequence annotation: -35
sequence annotation: BBa_R0011
sequence annotation: cei
sequence: https://synbiohub.org/public/igem/BBa_K914001_sequence/1

lac O1
type: SequenceAnnotation
persistent identity: annotation2188654
display id: annotation2188654
version: 1
top level: pLac ColE2
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188654/range2188654/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188654/range2188654/1
type: Range
persistent identity: range2188654
display id: range2188654
version: 1
direction: direction/forward
top level: pLac ColE2
start: 3
end: 19
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2188656
display id: annotation2188656
version: 1
top level: pLac ColE2
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188656/range2188656/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188656/range2188656/1
type: Range
persistent identity: range2188656
display id: range2188656
version: 1
direction: direction/forward
top level: pLac ColE2
start: 26
end: 42
orientation: inline

BBa_K914002
type: SequenceAnnotation
persistent identity: annotation2188659
display id: annotation2188659
version: 1
top level: pLac ColE2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188659/range2188659/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188659/range2188659/1
type: Range
persistent identity: range2188659
display id: range2188659
version: 1
direction: direction/reverse
top level: pLac ColE2
start: 64
end: 345
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2188657
display id: annotation2188657
version: 1
top level: pLac ColE2
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188657/range2188657/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188657/range2188657/1
type: Range
persistent identity: range2188657
display id: range2188657
version: 1
direction: direction/forward
top level: pLac ColE2
start: 43
end: 48
orientation: inline

repressilator
type: SequenceAnnotation
persistent identity: annotation2188658
display id: annotation2188658
version: 1
top level: pLac ColE2
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188658/range2188658/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188658/range2188658/1
type: Range
persistent identity: range2188658
display id: range2188658
version: 1
direction: direction/forward
top level: pLac ColE2
start: 64
end: 84
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2188655
display id: annotation2188655
version: 1
top level: pLac ColE2
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188655/range2188655/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188655/range2188655/1
type: Range
persistent identity: range2188655
display id: range2188655
version: 1
direction: direction/forward
top level: pLac ColE2
start: 20
end: 25
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2188653
display id: annotation2188653
version: 1
top level: pLac ColE2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188653/range2188653/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188653/range2188653/1
type: Range
persistent identity: range2188653
display id: range2188653
version: 1
direction: direction/forward
top level: pLac ColE2
start: 1
end: 54
orientation: inline

cei
type: SequenceAnnotation
persistent identity: annotation2188660
display id: annotation2188660
version: 1
top level: pLac ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188660/range2188660/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188660/range2188660/1
type: Range
persistent identity: range2188660
display id: range2188660
version: 1
direction: direction/forward
top level: pLac ColE2
start: 85
end: 345
orientation: inline

BBa_K864203
type: ComponentDefinition
persistent identity: BBa K864203
display id: BBa_K864203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA3 super-repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lexA3
mutable notes: lexA3 is lexA (BBa_K864201) with a G85D mutation (G>A basepair change at position 254)
mutable provenance: BBa_K864201
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lexA3
sequence annotation: G95D
sequence annotation: G>A
sequence: https://synbiohub.org/public/igem/BBa_K864203_sequence/1

lexA3
type: SequenceAnnotation
persistent identity: annotation2197171
display id: annotation2197171
version: 1
top level: BBa_K864203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197171/range2197171/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197171/range2197171/1
type: Range
persistent identity: range2197171
display id: range2197171
version: 1
direction: direction/forward
top level: BBa_K864203
start: 1
end: 612
orientation: inline

G95D
type: SequenceAnnotation
persistent identity: annotation2197172
display id: annotation2197172
version: 1
top level: BBa_K864203
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197172/range2197172/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197172/range2197172/1
type: Range
persistent identity: range2197172
display id: range2197172
version: 1
direction: direction/forward
top level: BBa_K864203
start: 253
end: 255
orientation: inline

G>A
type: SequenceAnnotation
persistent identity: annotation2197173
display id: annotation2197173
version: 1
top level: BBa_K864203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197173/range2197173/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197173/range2197173/1
type: Range
persistent identity: range2197173
display id: range2197173
version: 1
direction: direction/forward
top level: BBa_K864203
start: 254
end: 254
orientation: inline

BBa_K567016
type: ComponentDefinition
persistent identity: BBa K567016
display id: BBa_K567016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metY-CGA
creator: You Wang
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This biobrick is constructed by mutating the anticodon of tRNA（met） to TCG (base pairing codon CGA). This tRNA can transfer fMet to CGA when it is used as the start codon. Kana gene with start codon substituted for CGA is used to testify the function of metY-CGA. When this biobrick and metGM(BBa_K567014) or metG(BBa_K567015) are co-transformed into the cell, the cells can survive on the LBKana plate.
mutable notes: tRNA(met) has been involved in many operons, we chose metY
mutable provenance: tRNA（met）from metY in E.coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: tRNA-Met
sequence annotation: -35
sequence annotation: polya
sequence annotation: CAT->TCG
sequence annotation: TE
sequence annotation: PmetY
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K567016_sequence/1

tRNA-Met
type: SequenceAnnotation
persistent identity: annotation2153170
display id: annotation2153170
version: 1
top level: BBa_K567016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153170/range2153170/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153170/range2153170/1
type: Range
persistent identity: range2153170
display id: range2153170
version: 1
direction: direction/forward
top level: BBa_K567016
start: 273
end: 349
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2153187
display id: annotation2153187
version: 1
top level: BBa_K567016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153187/range2153187/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153187/range2153187/1
type: Range
persistent identity: range2153187
display id: range2153187
version: 1
direction: direction/forward
top level: BBa_K567016
start: 151
end: 156
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2153280
display id: annotation2153280
version: 1
top level: BBa_K567016
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153280/range2153280/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153280/range2153280/1
type: Range
persistent identity: range2153280
display id: range2153280
version: 1
direction: direction/forward
top level: BBa_K567016
start: 491
end: 496
orientation: inline

CAT->TCG
type: SequenceAnnotation
persistent identity: annotation2153173
display id: annotation2153173
version: 1
top level: BBa_K567016
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153173/range2153173/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153173/range2153173/1
type: Range
persistent identity: range2153173
display id: range2153173
version: 1
direction: direction/forward
top level: BBa_K567016
start: 307
end: 309
orientation: inline

TE
type: SequenceAnnotation
persistent identity: annotation2153214
display id: annotation2153214
version: 1
top level: BBa_K567016
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153214/range2153214/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153214/range2153214/1
type: Range
persistent identity: range2153214
display id: range2153214
version: 1
direction: direction/forward
top level: BBa_K567016
start: 475
end: 490
orientation: inline

PmetY
type: SequenceAnnotation
persistent identity: annotation2153197
display id: annotation2153197
version: 1
top level: BBa_K567016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153197/range2153197/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153197/range2153197/1
type: Range
persistent identity: range2153197
display id: range2153197
version: 1
direction: direction/forward
top level: BBa_K567016
start: 151
end: 186
orientation: inline

TE
type: SequenceAnnotation
persistent identity: annotation2153244
display id: annotation2153244
version: 1
top level: BBa_K567016
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153244/range2153244/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153244/range2153244/1
type: Range
persistent identity: range2153244
display id: range2153244
version: 1
direction: direction/reverse
top level: BBa_K567016
start: 519
end: 531
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2153293
display id: annotation2153293
version: 1
top level: BBa_K567016
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153293/range2153293/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153293/range2153293/1
type: Range
persistent identity: range2153293
display id: range2153293
version: 1
direction: direction/forward
top level: BBa_K567016
start: 532
end: 538
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2153186
display id: annotation2153186
version: 1
top level: BBa_K567016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567016/annotation2153186/range2153186/1

https://synbiohub.org/public/igem/BBa_K567016/annotation2153186/range2153186/1
type: Range
persistent identity: range2153186
display id: range2153186
version: 1
direction: direction/forward
top level: BBa_K567016
start: 175
end: 181
orientation: inline

BBa_K731722
type: ComponentDefinition
persistent identity: BBa K731722
display id: BBa_K731722
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T1 terminator from E. coli rrnB
creator: Anna Depetris, Giacomo Giacomelli
created: 2012-08-22T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the B0010 part extracted by PCR from the doulbe terminator B0012. We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.
mutable notes: I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
mutable provenance: This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_K731722_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation2180631
display id: annotation2180631
version: 1
top level: BBa_K731722
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731722/annotation2180631/range2180631/1

https://synbiohub.org/public/igem/BBa_K731722/annotation2180631/range2180631/1
type: Range
persistent identity: range2180631
display id: range2180631
version: 1
direction: direction/forward
top level: BBa_K731722
start: 1
end: 79
orientation: inline

BBa_S04616
type: ComponentDefinition
persistent identity: BBa S04616
display id: BBa_S04616
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:K592004
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:14:42Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K592004
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K592004
sequence: https://synbiohub.org/public/igem/BBa_S04616_sequence/1

BBa_K592004
type: Component
persistent identity: component2136950
display id: component2136950
version: 1
top level: BBa_S04616
access: public
definition: YF1

BBa_B0034
type: Component
persistent identity: component2136948
display id: component2136948
version: 1
top level: BBa_S04616
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136948
display id: annotation2136948
version: 1
top level: BBa_S04616
location: https://synbiohub.org/public/igem/BBa_S04616/annotation2136948/range2136948/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04616/annotation2136948/range2136948/1
type: Range
persistent identity: range2136948
display id: range2136948
version: 1
direction: direction/reverse
top level: BBa_S04616
start: 1
end: 12
orientation: inline

BBa_K592004
type: SequenceAnnotation
persistent identity: annotation2136950
display id: annotation2136950
version: 1
top level: BBa_S04616
location: https://synbiohub.org/public/igem/BBa_S04616/annotation2136950/range2136950/1
component: BBa_K592004

https://synbiohub.org/public/igem/BBa_S04616/annotation2136950/range2136950/1
type: Range
persistent identity: range2136950
display id: range2136950
version: 1
direction: direction/reverse
top level: BBa_S04616
start: 19
end: 1149
orientation: inline

BBa_K823008
type: ComponentDefinition
persistent identity: BBa K823008
display id: BBa_K823008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23106
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: J23106
sequence: https://synbiohub.org/public/igem/BBa_K823008_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182529
display id: annotation2182529
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
type: Range
persistent identity: range2182529
display id: range2182529
version: 1
direction: direction/forward
top level: BBa_K823008
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182528
display id: annotation2182528
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
type: Range
persistent identity: range2182528
display id: range2182528
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 6
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2182530
display id: annotation2182530
version: 1
top level: BBa_K823008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
type: Range
persistent identity: range2182530
display id: range2182530
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 35
orientation: inline

BBa_K588001
type: ComponentDefinition
persistent identity: BBa K588001
display id: BBa_K588001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ptrp
creator: Kevin Lu
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _759_
m user id: 0
owner id: 9140
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tryptophan promoter and operator
mutable notes: The sequence contains SpeI sites at positions critical for function. We modified the SpeI site in the biobrick suffix to an NheI site as done by UNAM 2010
mutable provenance: Synthesized by IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ptrpL
sequence annotation: ptrpO
sequence: https://synbiohub.org/public/igem/BBa_K588001_sequence/1

ptrpL
type: SequenceAnnotation
persistent identity: annotation2142514
display id: annotation2142514
version: 1
top level: BBa_K588001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K588001/annotation2142514/range2142514/1

https://synbiohub.org/public/igem/BBa_K588001/annotation2142514/range2142514/1
type: Range
persistent identity: range2142514
display id: range2142514
version: 1
direction: direction/forward
top level: BBa_K588001
start: 1
end: 49
orientation: inline

ptrpO
type: SequenceAnnotation
persistent identity: annotation2142550
display id: annotation2142550
version: 1
top level: BBa_K588001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K588001/annotation2142550/range2142550/1

https://synbiohub.org/public/igem/BBa_K588001/annotation2142550/range2142550/1
type: Range
persistent identity: range2142550
display id: range2142550
version: 1
direction: direction/forward
top level: BBa_K588001
start: 20
end: 40
orientation: inline

ThiE
type: ComponentDefinition
persistent identity: BBa K558009
display id: BBa_K558009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Thiamin monophosphate pyrophosphorylase
creator: Laura Colgin, Megan Tabor
created: 2011-09-22T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _726_
m user id: 0
owner id: 8801
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region of Thiamine E from synechocystis
mutable notes: coming soon
mutable provenance: Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: ThiE coding region
sequence: https://synbiohub.org/public/igem/BBa_K558009_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2144392
display id: annotation2144392
version: 1
top level: ThiE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K558009/annotation2144392/range2144392/1

https://synbiohub.org/public/igem/BBa_K558009/annotation2144392/range2144392/1
type: Range
persistent identity: range2144392
display id: range2144392
version: 1
direction: direction/forward
top level: ThiE
start: 1
end: 3
orientation: inline

ThiE coding region
type: SequenceAnnotation
persistent identity: annotation2144393
display id: annotation2144393
version: 1
top level: ThiE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K558009/annotation2144393/range2144393/1

https://synbiohub.org/public/igem/BBa_K558009/annotation2144393/range2144393/1
type: Range
persistent identity: range2144393
display id: range2144393
version: 1
direction: direction/forward
top level: ThiE
start: 1
end: 1032
orientation: inline

BBa_K325259
type: ComponentDefinition
persistent identity: BBa K325259
display id: BBa_K325259
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yellow Firefly Luciferase (under pBAD) L. Cruciata (E. coli optimised)
creator: Ben Reeve and Emily Knott
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: rbs
sequence annotation: Yellow Luciferase (452) (L. cruciata)
sequence annotation: PC
sequence annotation: AraC
sequence annotation: Pbad
sequence annotation: double stop codon
sequence annotation: BamH1 site
sequence annotation: Pro452Ser
sequence annotation: Luciferin Regenerating Enzyme (L. cruciata)
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K325259_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2103829
display id: annotation2103829
version: 1
top level: BBa_K325259
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103829/range2103829/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103829/range2103829/1
type: Range
persistent identity: range2103829
display id: range2103829
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1219
end: 1235
orientation: inline

Yellow Luciferase (452) (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103835
display id: annotation2103835
version: 1
top level: BBa_K325259
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103835/range2103835/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103835/range2103835/1
type: Range
persistent identity: range2103835
display id: range2103835
version: 1
direction: direction/forward
top level: BBa_K325259
start: 2192
end: 3835
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2103821
display id: annotation2103821
version: 1
top level: BBa_K325259
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103821/range2103821/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103821/range2103821/1
type: Range
persistent identity: range2103821
display id: range2103821
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1043
end: 1114
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2103820
display id: annotation2103820
version: 1
top level: BBa_K325259
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103820/range2103820/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103820/range2103820/1
type: Range
persistent identity: range2103820
display id: range2103820
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1
end: 1079
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2103828
display id: annotation2103828
version: 1
top level: BBa_K325259
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103828/range2103828/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103828/range2103828/1
type: Range
persistent identity: range2103828
display id: range2103828
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1080
end: 1210
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103834
display id: annotation2103834
version: 1
top level: BBa_K325259
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103834/range2103834/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103834/range2103834/1
type: Range
persistent identity: range2103834
display id: range2103834
version: 1
direction: direction/forward
top level: BBa_K325259
start: 2175
end: 2191
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103836
display id: annotation2103836
version: 1
top level: BBa_K325259
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103836/range2103836/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103836/range2103836/1
type: Range
persistent identity: range2103836
display id: range2103836
version: 1
direction: direction/forward
top level: BBa_K325259
start: 3836
end: 3841
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2103833
display id: annotation2103833
version: 1
top level: BBa_K325259
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103833/range2103833/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103833/range2103833/1
type: Range
persistent identity: range2103833
display id: range2103833
version: 1
direction: direction/forward
top level: BBa_K325259
start: 2169
end: 2174
orientation: inline

Pro452Ser
type: SequenceAnnotation
persistent identity: annotation2103893
display id: annotation2103893
version: 1
top level: BBa_K325259
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103893/range2103893/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103893/range2103893/1
type: Range
persistent identity: range2103893
display id: range2103893
version: 1
direction: direction/forward
top level: BBa_K325259
start: 3545
end: 3545
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103832
display id: annotation2103832
version: 1
top level: BBa_K325259
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103832/range2103832/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103832/range2103832/1
type: Range
persistent identity: range2103832
display id: range2103832
version: 1
direction: direction/forward
top level: BBa_K325259
start: 2163
end: 2168
orientation: inline

Luciferin Regenerating Enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103831
display id: annotation2103831
version: 1
top level: BBa_K325259
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103831/range2103831/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103831/range2103831/1
type: Range
persistent identity: range2103831
display id: range2103831
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1236
end: 2162
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2103819
display id: annotation2103819
version: 1
top level: BBa_K325259
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103819/range2103819/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103819/range2103819/1
type: Range
persistent identity: range2103819
display id: range2103819
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1
end: 1210
orientation: inline

BBa_K611012
type: ComponentDefinition
persistent identity: BBa K611012
display id: BBa_K611012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet Characterization Construct
creator: Nicholas Csicsery
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used to characterize mutants of BBa_R0040 (pTet). By adding a mutant of BBa_R0040 before this part, one can measure several of its properties through the expression of GFP. Certain characteristics of the mutant, such as the binding of the TetR repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.
mutable notes: There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.
mutable provenance: Built from existing parts in the registry though BioBrick assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_E0040
component: BBa_B0034
component: BBa_C0040
component: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K611012_sequence/1

BBa_B0015
type: Component
persistent identity: component2268733
display id: component2268733
version: 1
top level: BBa_K611012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E0040
type: Component
persistent identity: component2268726
display id: component2268726
version: 1
top level: BBa_K611012
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component2268724
display id: component2268724
version: 1
top level: BBa_K611012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2268736
display id: component2268736
version: 1
top level: BBa_K611012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0040
type: Component
persistent identity: component2268740
display id: component2268740
version: 1
top level: BBa_K611012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_I13453
type: Component
persistent identity: component2268734
display id: component2268734
version: 1
top level: BBa_K611012
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2268736
display id: annotation2268736
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268736/range2268736/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611012/annotation2268736/range2268736/1
type: Range
persistent identity: range2268736
display id: range2268736
version: 1
direction: direction/reverse
top level: BBa_K611012
start: 1022
end: 1033
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2268740
display id: annotation2268740
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268740/range2268740/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K611012/annotation2268740/range2268740/1
type: Range
persistent identity: range2268740
display id: range2268740
version: 1
direction: direction/reverse
top level: BBa_K611012
start: 1040
end: 1724
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2268726
display id: annotation2268726
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268726/range2268726/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K611012/annotation2268726/range2268726/1
type: Range
persistent identity: range2268726
display id: range2268726
version: 1
direction: direction/reverse
top level: BBa_K611012
start: 19
end: 738
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2268724
display id: annotation2268724
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268724/range2268724/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611012/annotation2268724/range2268724/1
type: Range
persistent identity: range2268724
display id: range2268724
version: 1
direction: direction/reverse
top level: BBa_K611012
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2268733
display id: annotation2268733
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268733/range2268733/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K611012/annotation2268733/range2268733/1
type: Range
persistent identity: range2268733
display id: range2268733
version: 1
direction: direction/reverse
top level: BBa_K611012
start: 747
end: 875
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2268734
display id: annotation2268734
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268734/range2268734/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K611012/annotation2268734/range2268734/1
type: Range
persistent identity: range2268734
display id: range2268734
version: 1
direction: direction/reverse
top level: BBa_K611012
start: 884
end: 1013
orientation: inline

LovTAP-VP1
type: ComponentDefinition
persistent identity: BBa K838000
display id: BBa_K838000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mammalian LovTAP-VP16
creator: Nicolas Gobet
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1097_
m user id: 0
owner id: 12458
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Melanopsin is a photosensitive protein that triggers a signalling cascade in mammalian cells.
mutable notes: Do not use PstI as restriction enzyme!!
mutable provenance: http://www.sciencemag.org/content/332/6037/1565.abstract
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K838000_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2194630
display id: annotation2194630
version: 1
top level: LovTAP-VP1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K838000/annotation2194630/range2194630/1

https://synbiohub.org/public/igem/BBa_K838000/annotation2194630/range2194630/1
type: Range
persistent identity: range2194630
display id: range2194630
version: 1
direction: direction/forward
top level: LovTAP-VP1
start: 28
end: 850
orientation: inline

BBa_K763002
type: ComponentDefinition
persistent identity: BBa K763002
display id: BBa_K763002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pNirB + Gene encoding ZsGreen1
creator: Pedro Luis Dorado Morales
created: 2012-09-06T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1014_
m user id: 0
owner id: 11756
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: When our bacteria are growing in absence of oxygen, ZsYellow1 gets expressed. Why? FNR, is a DNA binding protein that regulates a large family of genes involved in cellular respiration and carbon metabolism during conditions of anaerobic cell growth. That is because FNR proteins are sensitive to little fluctuations in environmental oxygen concentration (FNR is believed to contain a redox/O2-sensitive element for detecting the anaerobic state). These proteins recognise a DNA target consisting of an inverted repeat: TTGATN1N2N3N4ATCAA (where N 1-4 represents a non-conserved tetrad, NCT). Then, when there is no oxygen in the media, FNR proteins bind to promoter and the transcription is activated
mutable notes: No design considerations needed.
mutable provenance: The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K763002_sequence/1

BBa_K763001
type: ComponentDefinition
persistent identity: BBa K763001
display id: BBa_K763001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGroE + Gene encoding AsRed2
creator: Pedro Luis Dorado Morales
created: 2012-09-06T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1014_
m user id: 0
owner id: 11756
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: When the bacteria suffer heat shock the protein gets expressed. Why? Heat-shock response is mediated by the Sigma 32 factor. This alternative sigma factor lets the RNA polymerase binds to some consensus promoter sequence. groE has this sequence in the promoter. That is because this gene encodes a chaperon protein, GroE. Chaperone proteins are a group of proteins present in all cells, many of them are heat shock proteins, whose function is to assist the folding of other proteins in the newly formed protein synthesis. In the case of GroE, it processes a nonnative polypeptide in a cycle consisting of three steps. First, the polypeptide substrate is captured by GroEL. Upon binding of the co-chaperone GroES and ATP, the substrate is then discharged into a unique microenvironment inside of the chaperone, which promotes proper folding. After hydrolysis of ATP, the polypeptide is released into solution. Moreover, GroE may actively increase the folding efficiency, e.g. by unfolding of misfolded protein molecules. This chaperon has an important role in heat shock proccess too, helping other proteins not to denature.
mutable notes: No design considerations needed.
mutable provenance: The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AsRed2 fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K763001_sequence/1

BBa_K823002
type: ComponentDefinition
persistent identity: BBa K823002
display id: BBa_K823002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlepA
creator: Korinna Kraft
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PlepA is the promoter of the PlepA gene of Bacillus subtilis. It is a constitutive promoter and does not contain a ribosome binding site.
mutable notes: no considerations
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K823002_sequence/1

BBa_K823001
type: ComponentDefinition
persistent identity: BBa K823001
display id: BBa_K823001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PliaI promoter of Bacillus subtilis
creator: Korinna Kraft
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: PliaI is the promoter of the PliaI gene of Bacillus subtilis, which is inducible by bacitracin. It does not contain a ribosome binding site.
mutable notes: no considerations
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: -10
sequence annotation: -35
sequence annotation: liaR-binding
sequence annotation: PliaI
sequence: https://synbiohub.org/public/igem/BBa_K823001_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation2202718
display id: annotation2202718
version: 1
top level: BBa_K823001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202718/range2202718/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202718/range2202718/1
type: Range
persistent identity: range2202718
display id: range2202718
version: 1
direction: direction/forward
top level: BBa_K823001
start: 197
end: 197
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202720
display id: annotation2202720
version: 1
top level: BBa_K823001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202720/range2202720/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202720/range2202720/1
type: Range
persistent identity: range2202720
display id: range2202720
version: 1
direction: direction/forward
top level: BBa_K823001
start: 185
end: 190
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202721
display id: annotation2202721
version: 1
top level: BBa_K823001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202721/range2202721/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202721/range2202721/1
type: Range
persistent identity: range2202721
display id: range2202721
version: 1
direction: direction/forward
top level: BBa_K823001
start: 160
end: 165
orientation: inline

liaR-binding
type: SequenceAnnotation
persistent identity: annotation2202717
display id: annotation2202717
version: 1
top level: BBa_K823001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202717/range2202717/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202717/range2202717/1
type: Range
persistent identity: range2202717
display id: range2202717
version: 1
direction: direction/forward
top level: BBa_K823001
start: 125
end: 159
orientation: inline

PliaI
type: SequenceAnnotation
persistent identity: annotation2202715
display id: annotation2202715
version: 1
top level: BBa_K823001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202715/range2202715/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202715/range2202715/1
type: Range
persistent identity: range2202715
display id: range2202715
version: 1
direction: direction/forward
top level: BBa_K823001
start: 1
end: 209
orientation: inline

BBa_K539641
type: ComponentDefinition
persistent identity: BBa K539641
display id: BBa_K539641
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a RBS (B0032)+ilvC with a RBS (B0032)+ilvD
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K539622
component: BBa_K539625
sequence annotation: BBa_K539622
sequence annotation: BBa_K539625
sequence: https://synbiohub.org/public/igem/BBa_K539641_sequence/1

BBa_K539622
type: Component
persistent identity: component2144217
display id: component2144217
version: 1
top level: BBa_K539641
access: public
definition: BBa_K539622

BBa_K539625
type: Component
persistent identity: component2144223
display id: component2144223
version: 1
top level: BBa_K539641
access: public
definition: https://synbiohub.org/public/igem/BBa_K539625/1

BBa_K539622
type: SequenceAnnotation
persistent identity: annotation2144217
display id: annotation2144217
version: 1
top level: BBa_K539641
location: https://synbiohub.org/public/igem/BBa_K539641/annotation2144217/range2144217/1
component: BBa_K539622

https://synbiohub.org/public/igem/BBa_K539641/annotation2144217/range2144217/1
type: Range
persistent identity: range2144217
display id: range2144217
version: 1
direction: direction/reverse
top level: BBa_K539641
start: 1
end: 1495
orientation: inline

BBa_K539625
type: SequenceAnnotation
persistent identity: annotation2144223
display id: annotation2144223
version: 1
top level: BBa_K539641
location: https://synbiohub.org/public/igem/BBa_K539641/annotation2144223/range2144223/1
component: BBa_K539625

https://synbiohub.org/public/igem/BBa_K539641/annotation2144223/range2144223/1
type: Range
persistent identity: range2144223
display id: range2144223
version: 1
direction: direction/reverse
top level: BBa_K539641
start: 1504
end: 3373
orientation: inline

BBa_K823003
type: ComponentDefinition
persistent identity: BBa K823003
display id: BBa_K823003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg
creator: Korinna Kraft
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pveg is a strong, constitutive promoter of Bacillus subtilis
mutable notes: no considerations
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K823003_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2190180
display id: annotation2190180
version: 1
top level: BBa_K823003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823003/annotation2190180/range2190180/1

https://synbiohub.org/public/igem/BBa_K823003/annotation2190180/range2190180/1
type: Range
persistent identity: range2190180
display id: range2190180
version: 1
direction: direction/forward
top level: BBa_K823003
start: 1
end: 237
orientation: inline

BBa_K325210
type: ComponentDefinition
persistent identity: BBa K325210
display id: BBa_K325210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red Firefly Luciferase and LRE L. Cruciata (E. coli optimised)
creator: Hannah Copley and Theo Sanderson
created: 2010-09-14T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is contains the coding sequence for a mutant light-emitting enzyme (luciferase) and luciferin regenerating enzyme (LRE) from the Japanese firefly, Luciola cruciata. It will make bacteria emit light at a reddish wavelength when placed under a promoter, provided the media is supplemented with luciferin. The luciferin regenerating enzyme will convert oxyluciferin into CHBT. CHBT is converted to more luciferin in the presence of D-cysteine.
mutable notes: Codon optimisation, making compatible with BioBrick assembly format.
mutable provenance: This was codon-optimised for E.coli and syntesised by DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Red luciferase (286) (L. cruciata)
sequence annotation: G to A: Ser286Asn
sequence annotation: rbs
sequence annotation: double stop codon
sequence annotation: BamH1 site
sequence annotation: Luciferin regenerating enzyme (L. cruciata)
sequence: https://synbiohub.org/public/igem/BBa_K325210_sequence/1

Red luciferase (286) (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2080154
display id: annotation2080154
version: 1
top level: BBa_K325210
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080154/range2080154/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080154/range2080154/1
type: Range
persistent identity: range2080154
display id: range2080154
version: 1
direction: direction/forward
top level: BBa_K325210
start: 974
end: 2617
orientation: inline

G to A: Ser286Asn
type: SequenceAnnotation
persistent identity: annotation2103099
display id: annotation2103099
version: 1
top level: BBa_K325210
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2103099/range2103099/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2103099/range2103099/1
type: Range
persistent identity: range2103099
display id: range2103099
version: 1
direction: direction/forward
top level: BBa_K325210
start: 1830
end: 1830
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2080149
display id: annotation2080149
version: 1
top level: BBa_K325210
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080149/range2080149/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080149/range2080149/1
type: Range
persistent identity: range2080149
display id: range2080149
version: 1
direction: direction/forward
top level: BBa_K325210
start: 1
end: 17
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2080153
display id: annotation2080153
version: 1
top level: BBa_K325210
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080153/range2080153/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080153/range2080153/1
type: Range
persistent identity: range2080153
display id: range2080153
version: 1
direction: direction/forward
top level: BBa_K325210
start: 957
end: 973
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2080155
display id: annotation2080155
version: 1
top level: BBa_K325210
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080155/range2080155/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080155/range2080155/1
type: Range
persistent identity: range2080155
display id: range2080155
version: 1
direction: direction/forward
top level: BBa_K325210
start: 2618
end: 2623
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2080152
display id: annotation2080152
version: 1
top level: BBa_K325210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080152/range2080152/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080152/range2080152/1
type: Range
persistent identity: range2080152
display id: range2080152
version: 1
direction: direction/forward
top level: BBa_K325210
start: 951
end: 956
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2080151
display id: annotation2080151
version: 1
top level: BBa_K325210
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080151/range2080151/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080151/range2080151/1
type: Range
persistent identity: range2080151
display id: range2080151
version: 1
direction: direction/forward
top level: BBa_K325210
start: 945
end: 950
orientation: inline

Luciferin regenerating enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2080150
display id: annotation2080150
version: 1
top level: BBa_K325210
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080150/range2080150/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080150/range2080150/1
type: Range
persistent identity: range2080150
display id: range2080150
version: 1
direction: direction/forward
top level: BBa_K325210
start: 18
end: 944
orientation: inline

BBa_K575045
type: ComponentDefinition
persistent identity: BBa K575045
display id: BBa_K575045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Genomic LasP. producing GFP along with constiutivley producing LasR
mutable notes: no
mutable provenance: from genome and registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575041
component: BBa_K575004
sequence annotation: BBa_K575041
sequence annotation: BBa_K575004
sequence: https://synbiohub.org/public/igem/BBa_K575045_sequence/1

BBa_K575041
type: Component
persistent identity: component2138539
display id: component2138539
version: 1
top level: BBa_K575045
access: public
definition: https://synbiohub.org/public/igem/BBa_K575041/1

BBa_K575004
type: Component
persistent identity: component2138545
display id: component2138545
version: 1
top level: BBa_K575045
access: public
definition: https://synbiohub.org/public/igem/BBa_K575004/1

BBa_K575041
type: SequenceAnnotation
persistent identity: annotation2138539
display id: annotation2138539
version: 1
top level: BBa_K575045
location: https://synbiohub.org/public/igem/BBa_K575045/annotation2138539/range2138539/1
component: BBa_K575041

https://synbiohub.org/public/igem/BBa_K575045/annotation2138539/range2138539/1
type: Range
persistent identity: range2138539
display id: range2138539
version: 1
direction: direction/reverse
top level: BBa_K575045
start: 1
end: 1266
orientation: inline

BBa_K575004
type: SequenceAnnotation
persistent identity: annotation2138545
display id: annotation2138545
version: 1
top level: BBa_K575045
location: https://synbiohub.org/public/igem/BBa_K575045/annotation2138545/range2138545/1
component: BBa_K575004

https://synbiohub.org/public/igem/BBa_K575045/annotation2138545/range2138545/1
type: Range
persistent identity: range2138545
display id: range2138545
version: 1
direction: direction/reverse
top level: BBa_K575045
start: 1275
end: 2061
orientation: inline

BBa_K567013
type: ComponentDefinition
persistent identity: BBa K567013
display id: BBa_K567013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tRNA(Asp)-TAG
creator: Bin Zhao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of lpp promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated.
mutable notes: the correct processing of tRNA should be ensured
mutable provenance: the tRNA(Asp) from AspV in E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: polya
sequence annotation: GTC->CTA
sequence annotation: TE
sequence annotation: -10
sequence annotation: -35
sequence annotation: tRNA-Asp
sequence annotation: PaspV
sequence: https://synbiohub.org/public/igem/BBa_K567013_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation2152879
display id: annotation2152879
version: 1
top level: BBa_K567013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K567013/annotation2152879/range2152879/1

https://synbiohub.org/public/igem/BBa_K567013/annotation2152879/range2152879/1
type: Range
persistent identity: range2152879
display id: range2152879
version: 1
direction: direction/forward
top level: BBa_K567013
start: 268
end: 274
orientation: inline

GTC->CTA
type: SequenceAnnotation
persistent identity: annotation2152877
display id: annotation2152877
version: 1
top level: BBa_K567013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567013/annotation2152877/range2152877/1

https://synbiohub.org/public/igem/BBa_K567013/annotation2152877/range2152877/1
type: Range
persistent identity: range2152877
display id: range2152877
version: 1
direction: direction/forward
top level: BBa_K567013
start: 172
end: 174
orientation: inline

TE
type: SequenceAnnotation
persistent identity: annotation2152878
display id: annotation2152878
version: 1
top level: BBa_K567013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567013/annotation2152878/range2152878/1

https://synbiohub.org/public/igem/BBa_K567013/annotation2152878/range2152878/1
type: Range
persistent identity: range2152878
display id: range2152878
version: 1
direction: direction/forward
top level: BBa_K567013
start: 245
end: 267
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2152874
display id: annotation2152874
version: 1
top level: BBa_K567013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567013/annotation2152874/range2152874/1

https://synbiohub.org/public/igem/BBa_K567013/annotation2152874/range2152874/1
type: Range
persistent identity: range2152874
display id: range2152874
version: 1
direction: direction/forward
top level: BBa_K567013
start: 104
end: 111
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2152871
display id: annotation2152871
version: 1
top level: BBa_K567013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567013/annotation2152871/range2152871/1

https://synbiohub.org/public/igem/BBa_K567013/annotation2152871/range2152871/1
type: Range
persistent identity: range2152871
display id: range2152871
version: 1
direction: direction/forward
top level: BBa_K567013
start: 80
end: 88
orientation: inline

tRNA-Asp
type: SequenceAnnotation
persistent identity: annotation2152876
display id: annotation2152876
version: 1
top level: BBa_K567013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567013/annotation2152876/range2152876/1

https://synbiohub.org/public/igem/BBa_K567013/annotation2152876/range2152876/1
type: Range
persistent identity: range2152876
display id: range2152876
version: 1
direction: direction/forward
top level: BBa_K567013
start: 138
end: 214
orientation: inline

PaspV
type: SequenceAnnotation
persistent identity: annotation2152873
display id: annotation2152873
version: 1
top level: BBa_K567013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567013/annotation2152873/range2152873/1

https://synbiohub.org/public/igem/BBa_K567013/annotation2152873/range2152873/1
type: Range
persistent identity: range2152873
display id: range2152873
version: 1
direction: direction/forward
top level: BBa_K567013
start: 80
end: 119
orientation: inline

BBa_K606053
type: ComponentDefinition
persistent identity: BBa K606053
display id: BBa_K606053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tyrosine amber supressor tRNA double terminator
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tyrosine amber suppressor tRNA for B. Subtilis and double terminator.
mutable notes: biobrick assembly
mutable provenance: b
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_K606034
sequence annotation: BBa_B0015
sequence annotation: BBa_K606034
sequence: https://synbiohub.org/public/igem/BBa_K606053_sequence/1

BBa_B0015
type: Component
persistent identity: component2133141
display id: component2133141
version: 1
top level: BBa_K606053
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K606034
type: Component
persistent identity: component2133134
display id: component2133134
version: 1
top level: BBa_K606053
access: public
definition: Y taRNA

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2133141
display id: annotation2133141
version: 1
top level: BBa_K606053
location: https://synbiohub.org/public/igem/BBa_K606053/annotation2133141/range2133141/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K606053/annotation2133141/range2133141/1
type: Range
persistent identity: range2133141
display id: range2133141
version: 1
direction: direction/reverse
top level: BBa_K606053
start: 94
end: 222
orientation: inline

BBa_K606034
type: SequenceAnnotation
persistent identity: annotation2133134
display id: annotation2133134
version: 1
top level: BBa_K606053
location: https://synbiohub.org/public/igem/BBa_K606053/annotation2133134/range2133134/1
component: BBa_K606034

https://synbiohub.org/public/igem/BBa_K606053/annotation2133134/range2133134/1
type: Range
persistent identity: range2133134
display id: range2133134
version: 1
direction: direction/reverse
top level: BBa_K606053
start: 1
end: 85
orientation: inline

M256I CysE
type: ComponentDefinition
persistent identity: BBa K731010
display id: BBa_K731010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M256I CysE (Serine Acetyltransferase)
creator: Jason Fontana, Daniele Rossetto, Giacomo Giacomelli
created: 2012-08-19T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes. This mutated version results to be not inhibited by Cys overproduction, as it secretes Cys.
mutable notes: The construct has a strong RBS (AGGAGG) spaced 8nt from the start codon.
mutable provenance: PCR extraction from E. coli genome and mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop
sequence annotation: Spacer
sequence annotation: Start
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K731010_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2180205
display id: annotation2180205
version: 1
top level: M256I CysE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K731010/annotation2180205/range2180205/1

https://synbiohub.org/public/igem/BBa_K731010/annotation2180205/range2180205/1
type: Range
persistent identity: range2180205
display id: range2180205
version: 1
direction: direction/reverse
top level: M256I CysE
start: 834
end: 839
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2180202
display id: annotation2180202
version: 1
top level: M256I CysE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731010/annotation2180202/range2180202/1

https://synbiohub.org/public/igem/BBa_K731010/annotation2180202/range2180202/1
type: Range
persistent identity: range2180202
display id: range2180202
version: 1
direction: direction/forward
top level: M256I CysE
start: 7
end: 14
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2180204
display id: annotation2180204
version: 1
top level: M256I CysE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K731010/annotation2180204/range2180204/1

https://synbiohub.org/public/igem/BBa_K731010/annotation2180204/range2180204/1
type: Range
persistent identity: range2180204
display id: range2180204
version: 1
direction: direction/forward
top level: M256I CysE
start: 15
end: 17
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2180201
display id: annotation2180201
version: 1
top level: M256I CysE
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K731010/annotation2180201/range2180201/1

https://synbiohub.org/public/igem/BBa_K731010/annotation2180201/range2180201/1
type: Range
persistent identity: range2180201
display id: range2180201
version: 1
direction: direction/forward
top level: M256I CysE
start: 1
end: 6
orientation: inline

f1 ori
type: ComponentDefinition
persistent identity: BBa K314110
display id: BBa_K314110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: f1 origin
creator: IGEM 2010 Team Washington
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage
mutable provenance: Pet 29 vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: f1 origin
sequence: https://synbiohub.org/public/igem/BBa_K314110_sequence/1

f1 origin
type: SequenceAnnotation
persistent identity: annotation2115768
display id: annotation2115768
version: 1
top level: f1 ori
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K314110/annotation2115768/range2115768/1

https://synbiohub.org/public/igem/BBa_K314110/annotation2115768/range2115768/1
type: Range
persistent identity: range2115768
display id: range2115768
version: 1
direction: direction/forward
top level: f1 ori
start: 18
end: 324
orientation: inline

BBa_K575033
type: ComponentDefinition
persistent identity: BBa K575033
display id: BBa_K575033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive Promoter + RBS (B0034) + RhlR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Continuous expression of RhlR combined with receptor with RhlP.
mutable notes: put the constitutive promoter after the production of RhlR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575007
component: BBa_K575012
sequence annotation: BBa_K575007
sequence annotation: BBa_K575012
sequence: https://synbiohub.org/public/igem/BBa_K575033_sequence/1

BBa_K575007
type: Component
persistent identity: component2138363
display id: component2138363
version: 1
top level: BBa_K575033
access: public
definition: https://synbiohub.org/public/igem/BBa_K575007/1

BBa_K575012
type: Component
persistent identity: component2138358
display id: component2138358
version: 1
top level: BBa_K575033
access: public
definition: BBa_K575012

BBa_K575007
type: SequenceAnnotation
persistent identity: annotation2138363
display id: annotation2138363
version: 1
top level: BBa_K575033
location: https://synbiohub.org/public/igem/BBa_K575033/annotation2138363/range2138363/1
component: BBa_K575007

https://synbiohub.org/public/igem/BBa_K575033/annotation2138363/range2138363/1
type: Range
persistent identity: range2138363
display id: range2138363
version: 1
direction: direction/reverse
top level: BBa_K575033
start: 811
end: 1600
orientation: inline

BBa_K575012
type: SequenceAnnotation
persistent identity: annotation2138358
display id: annotation2138358
version: 1
top level: BBa_K575033
location: https://synbiohub.org/public/igem/BBa_K575033/annotation2138358/range2138358/1
component: BBa_K575012

https://synbiohub.org/public/igem/BBa_K575033/annotation2138358/range2138358/1
type: Range
persistent identity: range2138358
display id: range2138358
version: 1
direction: direction/reverse
top level: BBa_K575033
start: 1
end: 802
orientation: inline

BBa_K642002
type: ComponentDefinition
persistent identity: BBa K642002
display id: BBa_K642002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor tagged with yBFP
creator: Wilson Lam, Matthew Orton
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _818_
m user id: 0
owner id: 9288
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the cI repressor from phage lambda tagged with a yeast codon optimized BFP.
mutable notes: It was important that the BFP was codon optimized for yeast.
mutable provenance: The cI repressor was pcr amplified from a previously submitted part (BBa_K105004). The yeast codon optimized BFP was synthesized by Mr. Gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BFP ORF
sequence annotation: cI ORF
sequence annotation: XbaI/SpeI mixed site
sequence: https://synbiohub.org/public/igem/BBa_K642002_sequence/1

BFP ORF
type: SequenceAnnotation
persistent identity: annotation2138667
display id: annotation2138667
version: 1
top level: BBa_K642002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K642002/annotation2138667/range2138667/1

https://synbiohub.org/public/igem/BBa_K642002/annotation2138667/range2138667/1
type: Range
persistent identity: range2138667
display id: range2138667
version: 1
direction: direction/forward
top level: BBa_K642002
start: 720
end: 1418
orientation: inline

cI ORF
type: SequenceAnnotation
persistent identity: annotation2138643
display id: annotation2138643
version: 1
top level: BBa_K642002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K642002/annotation2138643/range2138643/1

https://synbiohub.org/public/igem/BBa_K642002/annotation2138643/range2138643/1
type: Range
persistent identity: range2138643
display id: range2138643
version: 1
direction: direction/forward
top level: BBa_K642002
start: 1
end: 713
orientation: inline

XbaI/SpeI mixed site
type: SequenceAnnotation
persistent identity: annotation2138666
display id: annotation2138666
version: 1
top level: BBa_K642002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K642002/annotation2138666/range2138666/1

https://synbiohub.org/public/igem/BBa_K642002/annotation2138666/range2138666/1
type: Range
persistent identity: range2138666
display id: range2138666
version: 1
direction: direction/forward
top level: BBa_K642002
start: 714
end: 719
orientation: inline

BBa_K316003
type: ComponentDefinition
persistent identity: BBa K316003
display id: BBa_K316003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylE - catechol 2,3-dioxygenase from P.putida with terminator
creator: IC 2010 Team
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:11:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Catechol or catechol 2,3-dioxygenases + O(2) is converted by a ring cleavage into 2-hydroxymuconate semialdehyde which is the toxic and bright yellow-coloured substrate. This is a key enzyme in many (soil) bacterial species used for the degradation of aromatic compounds. The enzyme used here itself originating from Pseudomonas putida is a homotetramer of C230 monomers. The tetramerization interactions position a ferrous ion critical for enzymatic activity. It has been deduced that intersubunit interaction is essential to produce a functioning enzyme after performing N and C terminal modifications on the monomer. Coming together the subunits generate an active site. The reaction itself takes place within seconds after the addition by Pasteur pipette or spraying of catechol at a 100mM stock solution diluted with DDH20 (used by our lab.) The toxic byproduct is thought to interfere with cell wall integrity and cellular machinery such that exposed cells gradually die.
mutable notes: The parts were put together using standard assembly [http://partsregistry.org/Assembly:Standard_assembly]
mutable provenance: XylE was obtained from the registry BBa_K118021 , the terminator is BBa_B0014
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0014
component: BBa_J33204
sequence annotation: BBa_B0014
sequence annotation: BBa_J33204
sequence: https://synbiohub.org/public/igem/BBa_K316003_sequence/1

BBa_B0014
type: Component
persistent identity: component2092342
display id: component2092342
version: 1
top level: BBa_K316003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_J33204
type: Component
persistent identity: component2092335
display id: component2092335
version: 1
top level: BBa_K316003
access: public
definition: https://synbiohub.org/public/igem/BBa_J33204/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092342
display id: annotation2092342
version: 1
top level: BBa_K316003
location: https://synbiohub.org/public/igem/BBa_K316003/annotation2092342/range2092342/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K316003/annotation2092342/range2092342/1
type: Range
persistent identity: range2092342
display id: range2092342
version: 1
direction: direction/reverse
top level: BBa_K316003
start: 967
end: 1061
orientation: inline

BBa_J33204
type: SequenceAnnotation
persistent identity: annotation2092335
display id: annotation2092335
version: 1
top level: BBa_K316003
location: https://synbiohub.org/public/igem/BBa_K316003/annotation2092335/range2092335/1
component: BBa_J33204

https://synbiohub.org/public/igem/BBa_K316003/annotation2092335/range2092335/1
type: Range
persistent identity: range2092335
display id: range2092335
version: 1
direction: direction/reverse
top level: BBa_K316003
start: 1
end: 958
orientation: inline

BBa_K598001
type: ComponentDefinition
persistent identity: BBa K598001
display id: BBa_K598001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
creator: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to regulate the translation of other genes.
mutable notes: The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
mutable provenance: Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Engineered RBS
sequence annotation: polya
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: Theophylline responsive riboswitch Theo 1G1
sequence annotation: stop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: eGFP
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K598001_sequence/1

Engineered RBS
type: SequenceAnnotation
persistent identity: annotation2152702
display id: annotation2152702
version: 1
top level: BBa_K598001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1
type: Range
persistent identity: range2152702
display id: range2152702
version: 1
direction: direction/forward
top level: BBa_K598001
start: 75
end: 81
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2152887
display id: annotation2152887
version: 1
top level: BBa_K598001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1
type: Range
persistent identity: range2152887
display id: range2152887
version: 1
direction: direction/forward
top level: BBa_K598001
start: 930
end: 943
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152703
display id: annotation2152703
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1
type: Range
persistent identity: range2152703
display id: range2152703
version: 1
direction: direction/forward
top level: BBa_K598001
start: 87
end: 806
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152882
display id: annotation2152882
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1
type: Range
persistent identity: range2152882
display id: range2152882
version: 1
direction: direction/forward
top level: BBa_K598001
start: 815
end: 894
orientation: inline

Theophylline responsive riboswitch Theo 1G1
type: SequenceAnnotation
persistent identity: annotation2152701
display id: annotation2152701
version: 1
top level: BBa_K598001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1
type: Range
persistent identity: range2152701
display id: range2152701
version: 1
direction: direction/forward
top level: BBa_K598001
start: 1
end: 86
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2152892
display id: annotation2152892
version: 1
top level: BBa_K598001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1
type: Range
persistent identity: range2152892
display id: range2152892
version: 1
direction: direction/forward
top level: BBa_K598001
start: 936
end: 936
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152705
display id: annotation2152705
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1
type: Range
persistent identity: range2152705
display id: range2152705
version: 1
direction: direction/forward
top level: BBa_K598001
start: 903
end: 943
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152901
display id: annotation2152901
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1
type: Range
persistent identity: range2152901
display id: range2152901
version: 1
direction: direction/forward
top level: BBa_K598001
start: 910
end: 929
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152704
display id: annotation2152704
version: 1
top level: BBa_K598001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1
type: Range
persistent identity: range2152704
display id: range2152704
version: 1
direction: direction/forward
top level: BBa_K598001
start: 87
end: 806
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152895
display id: annotation2152895
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1
type: Range
persistent identity: range2152895
display id: range2152895
version: 1
direction: direction/forward
top level: BBa_K598001
start: 826
end: 869
orientation: inline

BBa_K934022
type: ComponentDefinition
persistent identity: BBa K934022
display id: BBa_K934022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux-LasI
creator: Toshiki Hashimoto
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1199_
m user id: 0
owner id: 14028
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: We constructed this part by combining R0062 and K081016. This part starts to generate LasI protein in the presence of LuxR-3OC6HSL complex.
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K081016
sequence annotation: BBa_K081016
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K934022_sequence/1

BBa_R0062
type: Component
persistent identity: component2251021
display id: component2251021
version: 1
top level: BBa_K934022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K081016
type: Component
persistent identity: component2251038
display id: component2251038
version: 1
top level: BBa_K934022
access: public
definition: https://synbiohub.org/public/igem/BBa_K081016/1

BBa_K081016
type: SequenceAnnotation
persistent identity: annotation2251038
display id: annotation2251038
version: 1
top level: BBa_K934022
location: https://synbiohub.org/public/igem/BBa_K934022/annotation2251038/range2251038/1
component: BBa_K081016

https://synbiohub.org/public/igem/BBa_K934022/annotation2251038/range2251038/1
type: Range
persistent identity: range2251038
display id: range2251038
version: 1
direction: direction/reverse
top level: BBa_K934022
start: 64
end: 798
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2251021
display id: annotation2251021
version: 1
top level: BBa_K934022
location: https://synbiohub.org/public/igem/BBa_K934022/annotation2251021/range2251021/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K934022/annotation2251021/range2251021/1
type: Range
persistent identity: range2251021
display id: range2251021
version: 1
direction: direction/forward
top level: BBa_K934022
start: 1
end: 55
orientation: inline

BBa_K914003
type: ComponentDefinition
persistent identity: BBa K914003
display id: BBa_K914003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: L-rhamnose-inducible promoter (pRha)
creator: Denis Samuylov
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13487
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose.
mutable notes: One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.
mutable provenance: Amplification of the plasmid pJOE3075 (Dr. Altenbuchner).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pRha
sequence: https://synbiohub.org/public/igem/BBa_K914003_sequence/1

pRha
type: SequenceAnnotation
persistent identity: annotation2188559
display id: annotation2188559
version: 1
top level: BBa_K914003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1

https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
type: Range
persistent identity: range2188559
display id: range2188559
version: 1
direction: direction/forward
top level: BBa_K914003
start: 1
end: 122
orientation: inline

BBa_K819017
type: ComponentDefinition
persistent identity: BBa K819017
display id: BBa_K819017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible SulA408 promoter
creator: YU Zhou
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: SulA promoter in 408 form.SulA promoter belongs to the SOS regulon family, and promotes in E.coli transcription of SulA protein. SulA408 promoter is reponsive to our optimized Luminesensor(BBa_K819005).
mutable notes: SulA promoter in 408 form.
mutable provenance: SulA promoter sequence is got from regulon.org.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SulA408 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K819017_sequence/1

SulA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2197266
display id: annotation2197266
version: 1
top level: BBa_K819017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819017/annotation2197266/range2197266/1

https://synbiohub.org/public/igem/BBa_K819017/annotation2197266/range2197266/1
type: Range
persistent identity: range2197266
display id: range2197266
version: 1
direction: direction/forward
top level: BBa_K819017
start: 1
end: 81
orientation: inline

tphB
type: ComponentDefinition
persistent identity: BBa K808010
display id: BBa_K808010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphB: reaction from DCD to protocatechuate
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K808010_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2181625
display id: annotation2181625
version: 1
top level: tphB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808010/annotation2181625/range2181625/1

https://synbiohub.org/public/igem/BBa_K808010/annotation2181625/range2181625/1
type: Range
persistent identity: range2181625
display id: range2181625
version: 1
direction: direction/forward
top level: tphB
start: 1
end: 948
orientation: inline

BBa_K545666
type: ComponentDefinition
persistent identity: BBa K545666
display id: BBa_K545666
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rpos leader sequence
creator: Grenoble 2011
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _712_
m user id: 0
owner id: 8648
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This leader sequence originally from the 5'-untranslated RNA of the rpoS gene. Its particular secondary structure places the ribosome binding site into a double-stranded region and therefore prevents recognition by the ribosome. A small RNA, called dsrA can interacts with it and induces a change that liberates the RBS and thus stimulates the translation the downstream gene.
mutable notes: It has been extracted from colony by PCR and cloned into PSB1C3.
mutable provenance: This bricks comes from escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rpos
sequence: https://synbiohub.org/public/igem/BBa_K545666_sequence/1

rpos
type: SequenceAnnotation
persistent identity: annotation2137872
display id: annotation2137872
version: 1
top level: BBa_K545666
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K545666/annotation2137872/range2137872/1

https://synbiohub.org/public/igem/BBa_K545666/annotation2137872/range2137872/1
type: Range
persistent identity: range2137872
display id: range2137872
version: 1
direction: direction/forward
top level: BBa_K545666
start: 1
end: 223
orientation: inline

BBa_K823005
type: ComponentDefinition
persistent identity: BBa K823005
display id: BBa_K823005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23101
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:44:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: J23101
sequence: https://synbiohub.org/public/igem/BBa_K823005_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182511
display id: annotation2182511
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1
type: Range
persistent identity: range2182511
display id: range2182511
version: 1
direction: direction/forward
top level: BBa_K823005
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182510
display id: annotation2182510
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1
type: Range
persistent identity: range2182510
display id: range2182510
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 6
orientation: inline

J23101
type: SequenceAnnotation
persistent identity: annotation2182509
display id: annotation2182509
version: 1
top level: BBa_K823005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1
type: Range
persistent identity: range2182509
display id: range2182509
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 35
orientation: inline

BBa_K516032
type: ComponentDefinition
persistent identity: BBa K516032
display id: BBa_K516032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP protein generator TERM+ RBS+(B0032)
creator: Nicolo' Politi, Federica Sampietro, Davide Bianchini
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: mRFP protein generator TERM+ RBS+(B0032)
mutable notes: --
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0032
component: BBa_B0015
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K516032_sequence/1

BBa_B0032
type: Component
persistent identity: component2250597
display id: component2250597
version: 1
top level: BBa_K516032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0015
type: Component
persistent identity: component2250608
display id: component2250608
version: 1
top level: BBa_K516032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E1010
type: Component
persistent identity: component2250601
display id: component2250601
version: 1
top level: BBa_K516032
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250601
display id: annotation2250601
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250601/range2250601/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K516032/annotation2250601/range2250601/1
type: Range
persistent identity: range2250601
display id: range2250601
version: 1
direction: direction/reverse
top level: BBa_K516032
start: 20
end: 725
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250597
display id: annotation2250597
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250597/range2250597/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K516032/annotation2250597/range2250597/1
type: Range
persistent identity: range2250597
display id: range2250597
version: 1
direction: direction/forward
top level: BBa_K516032
start: 1
end: 13
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250608
display id: annotation2250608
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250608/range2250608/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516032/annotation2250608/range2250608/1
type: Range
persistent identity: range2250608
display id: range2250608
version: 1
direction: direction/reverse
top level: BBa_K516032
start: 734
end: 862
orientation: inline

BBa_K415023
type: ComponentDefinition
persistent identity: BBa K415023
display id: BBa_K415023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Plux/cI-OR:LuxI
creator: Andrew Yang
created: 2010-07-23T11:00:00Z
modified: 2015-05-08T01:12:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _525_
m user id: 0
owner id: 6124
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a LuxR/LacO-regulated GFP, LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification, TetR-regulated LuxR production.
mutable notes: to do
mutable provenance: to do
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K415022
component: BBa_K182101
sequence annotation: BBa_K182101
sequence annotation: BBa_K415022
sequence: https://synbiohub.org/public/igem/BBa_K415023_sequence/1

BBa_K415022
type: Component
persistent identity: component2318109
display id: component2318109
version: 1
top level: BBa_K415023
access: public
definition: https://synbiohub.org/public/igem/BBa_K415022/1

BBa_K182101
type: Component
persistent identity: component2318051
display id: component2318051
version: 1
top level: BBa_K415023
access: public
definition: https://synbiohub.org/public/igem/BBa_K182101/1

BBa_K182101
type: SequenceAnnotation
persistent identity: annotation2318051
display id: annotation2318051
version: 1
top level: BBa_K415023
location: https://synbiohub.org/public/igem/BBa_K415023/annotation2318051/range2318051/1
component: BBa_K182101

https://synbiohub.org/public/igem/BBa_K415023/annotation2318051/range2318051/1
type: Range
persistent identity: range2318051
display id: range2318051
version: 1
direction: direction/reverse
top level: BBa_K415023
start: 1
end: 960
orientation: inline

BBa_K415022
type: SequenceAnnotation
persistent identity: annotation2318109
display id: annotation2318109
version: 1
top level: BBa_K415023
location: https://synbiohub.org/public/igem/BBa_K415023/annotation2318109/range2318109/1
component: BBa_K415022

https://synbiohub.org/public/igem/BBa_K415023/annotation2318109/range2318109/1
type: Range
persistent identity: range2318109
display id: range2318109
version: 1
direction: direction/reverse
top level: BBa_K415023
start: 969
end: 3722
orientation: inline

BBa_K611013
type: ComponentDefinition
persistent identity: BBa K611013
display id: BBa_K611013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI Characterization Construct
creator: Nicholas Csicsery
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used to characterize mutants of BBa_R0010 (pLacI). By adding a mutant of BBa_R0010 before this part, one can measure several of its properties through the expression of GFP. Certain characteristics of the mutant, such as the binding of the LacI repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.
mutable notes: There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.
mutable provenance: Built from existing parts in the registry though BioBrick assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0040
component: BBa_C0012
component: BBa_B0015
component: BBa_I13453
sequence annotation: BBa_B0015
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_I13453
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K611013_sequence/1

BBa_B0034
type: Component
persistent identity: component2268754
display id: component2268754
version: 1
top level: BBa_K611013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2268744
display id: component2268744
version: 1
top level: BBa_K611013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_C0012
type: Component
persistent identity: component2268756
display id: component2268756
version: 1
top level: BBa_K611013
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0015
type: Component
persistent identity: component2268751
display id: component2268751
version: 1
top level: BBa_K611013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2268742
display id: component2268742
version: 1
top level: BBa_K611013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13453
type: Component
persistent identity: component2268752
display id: component2268752
version: 1
top level: BBa_K611013
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2268751
display id: annotation2268751
version: 1
top level: BBa_K611013
location: https://synbiohub.org/public/igem/BBa_K611013/annotation2268751/range2268751/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K611013/annotation2268751/range2268751/1
type: Range
persistent identity: range2268751
display id: range2268751
version: 1
direction: direction/reverse
top level: BBa_K611013
start: 747
end: 875
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2268744
display id: annotation2268744
version: 1
top level: BBa_K611013
location: https://synbiohub.org/public/igem/BBa_K611013/annotation2268744/range2268744/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K611013/annotation2268744/range2268744/1
type: Range
persistent identity: range2268744
display id: range2268744
version: 1
direction: direction/reverse
top level: BBa_K611013
start: 19
end: 738
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2268754
display id: annotation2268754
version: 1
top level: BBa_K611013
location: https://synbiohub.org/public/igem/BBa_K611013/annotation2268754/range2268754/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611013/annotation2268754/range2268754/1
type: Range
persistent identity: range2268754
display id: range2268754
version: 1
direction: direction/reverse
top level: BBa_K611013
start: 1022
end: 1033
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2268752
display id: annotation2268752
version: 1
top level: BBa_K611013
location: https://synbiohub.org/public/igem/BBa_K611013/annotation2268752/range2268752/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K611013/annotation2268752/range2268752/1
type: Range
persistent identity: range2268752
display id: range2268752
version: 1
direction: direction/reverse
top level: BBa_K611013
start: 884
end: 1013
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2268756
display id: annotation2268756
version: 1
top level: BBa_K611013
location: https://synbiohub.org/public/igem/BBa_K611013/annotation2268756/range2268756/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K611013/annotation2268756/range2268756/1
type: Range
persistent identity: range2268756
display id: range2268756
version: 1
direction: direction/forward
top level: BBa_K611013
start: 1040
end: 2167
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2268742
display id: annotation2268742
version: 1
top level: BBa_K611013
location: https://synbiohub.org/public/igem/BBa_K611013/annotation2268742/range2268742/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611013/annotation2268742/range2268742/1
type: Range
persistent identity: range2268742
display id: range2268742
version: 1
direction: direction/reverse
top level: BBa_K611013
start: 1
end: 12
orientation: inline

BBa_K546000
type: ComponentDefinition
persistent identity: BBa K546000
display id: BBa_K546000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. Complementary to the lux pL promoter, the lux pR promoter with a complex of luxR and 3OC6HSL will enable enlarged activation of the downstream synthetase gene, thus increase the concentration 3OC6HSL.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.
mutable provenance: The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F2621
component: BBa_K082014
sequence annotation: BBa_F2621
sequence annotation: BBa_K082014
sequence: https://synbiohub.org/public/igem/BBa_K546000_sequence/1

BBa_F2621
type: Component
persistent identity: component2289549
display id: component2289549
version: 1
top level: BBa_K546000
access: public
definition: https://synbiohub.org/public/igem/BBa_F2621/1

BBa_K082014
type: Component
persistent identity: component2289564
display id: component2289564
version: 1
top level: BBa_K546000
access: public
definition: https://synbiohub.org/public/igem/BBa_K082014/1

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2289549
display id: annotation2289549
version: 1
top level: BBa_K546000
location: https://synbiohub.org/public/igem/BBa_K546000/annotation2289549/range2289549/1
component: BBa_F2621

https://synbiohub.org/public/igem/BBa_K546000/annotation2289549/range2289549/1
type: Range
persistent identity: range2289549
display id: range2289549
version: 1
direction: direction/reverse
top level: BBa_K546000
start: 1
end: 1158
orientation: inline

BBa_K082014
type: SequenceAnnotation
persistent identity: annotation2289564
display id: annotation2289564
version: 1
top level: BBa_K546000
location: https://synbiohub.org/public/igem/BBa_K546000/annotation2289564/range2289564/1
component: BBa_K082014

https://synbiohub.org/public/igem/BBa_K546000/annotation2289564/range2289564/1
type: Range
persistent identity: range2289564
display id: range2289564
version: 1
direction: direction/reverse
top level: BBa_K546000
start: 1167
end: 1964
orientation: inline

BBa_K516022
type: ComponentDefinition
persistent identity: BBa K516022
display id: BBa_K516022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AiiA protein generator TERM+ RBS+(B0032)
creator: Susanna Zucca, Tommaso Goggia, Paolo Magni
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: AiiA protein generator TERM+ RBS+(B0032)
mutable notes: --
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_C0060
component: BBa_B0032
component: BBa_B0015
sequence annotation: BBa_B0032
sequence annotation: BBa_C0060
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K516022_sequence/1

BBa_C0060
type: Component
persistent identity: component2250560
display id: component2250560
version: 1
top level: BBa_K516022
access: public
definition: https://synbiohub.org/public/igem/BBa_C0060/1

BBa_B0032
type: Component
persistent identity: component2250556
display id: component2250556
version: 1
top level: BBa_K516022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0015
type: Component
persistent identity: component2250570
display id: component2250570
version: 1
top level: BBa_K516022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250556
display id: annotation2250556
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250556/range2250556/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K516022/annotation2250556/range2250556/1
type: Range
persistent identity: range2250556
display id: range2250556
version: 1
direction: direction/forward
top level: BBa_K516022
start: 1
end: 13
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation2250560
display id: annotation2250560
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250560/range2250560/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_K516022/annotation2250560/range2250560/1
type: Range
persistent identity: range2250560
display id: range2250560
version: 1
direction: direction/forward
top level: BBa_K516022
start: 20
end: 808
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250570
display id: annotation2250570
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250570/range2250570/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516022/annotation2250570/range2250570/1
type: Range
persistent identity: range2250570
display id: range2250570
version: 1
direction: direction/reverse
top level: BBa_K516022
start: 842
end: 970
orientation: inline

raiI
type: ComponentDefinition
persistent identity: BBa K594007
display id: BBa_K594007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a luxI homologous AHL synthetase from Rhzobium etli CFN 42
creator: Yu Xiaoyan
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _765_
m user id: 0
owner id: 8221
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RaiI is responsible for the synthesis of 3-OH-C8-HSL, a kind of AHL. The rai system in Rhzobium etli CFN42 can take the example of Rhizobium leguminosarum bv. vicae. RaiI is positively autoregulated by RaiR and 3-OH-C8-HSL but is also induced to a lesser extent by 3-OH-C14:1-HSL(produced by cinI) and 3-oxo-C8-HSL (produced by traI). These observations help explain the effect of cinRI mutations on the production of some of the short-chain AHLs and also suggest that an additional 3-oxo-C8-HSL-producing locus may contribute to regulation of raiRI.
mutable notes: no
mutable provenance: This part is from Rhzobium etli CFN42.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: raiI
sequence: https://synbiohub.org/public/igem/BBa_K594007_sequence/1

raiI
type: SequenceAnnotation
persistent identity: annotation2148105
display id: annotation2148105
version: 1
top level: raiI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K594007/annotation2148105/range2148105/1

https://synbiohub.org/public/igem/BBa_K594007/annotation2148105/range2148105/1
type: Range
persistent identity: range2148105
display id: range2148105
version: 1
direction: direction/forward
top level: raiI
start: 1
end: 639
orientation: inline

BBa_K554009
type: ComponentDefinition
persistent identity: BBa K554009
display id: BBa_K554009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TolC
creator: UNICAMP EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: TolC is ...
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: TolC
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K554009_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2136926
display id: annotation2136926
version: 1
top level: BBa_K554009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K554009/annotation2136926/range2136926/1

https://synbiohub.org/public/igem/BBa_K554009/annotation2136926/range2136926/1
type: Range
persistent identity: range2136926
display id: range2136926
version: 1
direction: direction/forward
top level: BBa_K554009
start: 1
end: 15
orientation: inline

TolC
type: SequenceAnnotation
persistent identity: annotation2136927
display id: annotation2136927
version: 1
top level: BBa_K554009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K554009/annotation2136927/range2136927/1

https://synbiohub.org/public/igem/BBa_K554009/annotation2136927/range2136927/1
type: Range
persistent identity: range2136927
display id: range2136927
version: 1
direction: direction/forward
top level: BBa_K554009
start: 16
end: 1533
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2136928
display id: annotation2136928
version: 1
top level: BBa_K554009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K554009/annotation2136928/range2136928/1

https://synbiohub.org/public/igem/BBa_K554009/annotation2136928/range2136928/1
type: Range
persistent identity: range2136928
display id: range2136928
version: 1
direction: direction/forward
top level: BBa_K554009
start: 1534
end: 1539
orientation: inline

BBa_K611016
type: ComponentDefinition
persistent identity: BBa K611016
display id: BBa_K611016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Repressor and Promoter Wild Type Control
creator: Nicholas Csicsery
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:53Z
discontinued: false
dominant: false
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is can be used as a control for BBa_K611010 and BBa_K611013, which test either C0012 and R0010 mutants respectively.
mutable notes: There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.
mutable provenance: Built from existing parts in the registry though BioBrick assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I13453
component: BBa_E0040
component: BBa_B0015
component: BBa_R0010
component: BBa_C0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_I13453
sequence annotation: BBa_R0010
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K611016_sequence/1

BBa_B0034
type: Component
persistent identity: component2273454
display id: component2273454
version: 1
top level: BBa_K611016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13453
type: Component
persistent identity: component2273452
display id: component2273452
version: 1
top level: BBa_K611016
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: Component
persistent identity: component2273442
display id: component2273442
version: 1
top level: BBa_K611016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2273444
display id: component2273444
version: 1
top level: BBa_K611016
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0015
type: Component
persistent identity: component2273451
display id: component2273451
version: 1
top level: BBa_K611016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: Component
persistent identity: component2273434
display id: component2273434
version: 1
top level: BBa_K611016
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0012
type: Component
persistent identity: component2273456
display id: component2273456
version: 1
top level: BBa_K611016
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273444
display id: annotation2273444
version: 1
top level: BBa_K611016
location: https://synbiohub.org/public/igem/BBa_K611016/annotation2273444/range2273444/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K611016/annotation2273444/range2273444/1
type: Range
persistent identity: range2273444
display id: range2273444
version: 1
direction: direction/reverse
top level: BBa_K611016
start: 227
end: 946
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273442
display id: annotation2273442
version: 1
top level: BBa_K611016
location: https://synbiohub.org/public/igem/BBa_K611016/annotation2273442/range2273442/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611016/annotation2273442/range2273442/1
type: Range
persistent identity: range2273442
display id: range2273442
version: 1
direction: direction/reverse
top level: BBa_K611016
start: 209
end: 220
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273454
display id: annotation2273454
version: 1
top level: BBa_K611016
location: https://synbiohub.org/public/igem/BBa_K611016/annotation2273454/range2273454/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611016/annotation2273454/range2273454/1
type: Range
persistent identity: range2273454
display id: range2273454
version: 1
direction: direction/reverse
top level: BBa_K611016
start: 1230
end: 1241
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273451
display id: annotation2273451
version: 1
top level: BBa_K611016
location: https://synbiohub.org/public/igem/BBa_K611016/annotation2273451/range2273451/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K611016/annotation2273451/range2273451/1
type: Range
persistent identity: range2273451
display id: range2273451
version: 1
direction: direction/reverse
top level: BBa_K611016
start: 955
end: 1083
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2273452
display id: annotation2273452
version: 1
top level: BBa_K611016
location: https://synbiohub.org/public/igem/BBa_K611016/annotation2273452/range2273452/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K611016/annotation2273452/range2273452/1
type: Range
persistent identity: range2273452
display id: range2273452
version: 1
direction: direction/reverse
top level: BBa_K611016
start: 1092
end: 1221
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2273434
display id: annotation2273434
version: 1
top level: BBa_K611016
location: https://synbiohub.org/public/igem/BBa_K611016/annotation2273434/range2273434/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K611016/annotation2273434/range2273434/1
type: Range
persistent identity: range2273434
display id: range2273434
version: 1
direction: direction/forward
top level: BBa_K611016
start: 1
end: 200
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2273456
display id: annotation2273456
version: 1
top level: BBa_K611016
location: https://synbiohub.org/public/igem/BBa_K611016/annotation2273456/range2273456/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K611016/annotation2273456/range2273456/1
type: Range
persistent identity: range2273456
display id: range2273456
version: 1
direction: direction/forward
top level: BBa_K611016
start: 1248
end: 2375
orientation: inline

BBa_K314100
type: ComponentDefinition
persistent identity: BBa K314100
display id: BBa_K314100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High constitutive expression cassette
creator: Tomas Huber, Justin Siegel
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: High constitutive protein expression insert includes f1 origin and RBS B0034
mutable provenance: construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K314110
sequence annotation: BBa_K314110
sequence: https://synbiohub.org/public/igem/BBa_K314100_sequence/1

BBa_K314110
type: Component
persistent identity: component2090995
display id: component2090995
version: 1
top level: BBa_K314100
access: public
definition: f1 ori

BBa_K314110
type: SequenceAnnotation
persistent identity: annotation2090995
display id: annotation2090995
version: 1
top level: BBa_K314100
location: https://synbiohub.org/public/igem/BBa_K314100/annotation2090995/range2090995/1
component: BBa_K314110

https://synbiohub.org/public/igem/BBa_K314100/annotation2090995/range2090995/1
type: Range
persistent identity: range2090995
display id: range2090995
version: 1
direction: direction/reverse
top level: BBa_K314100
start: 1
end: 455
orientation: inline

BBa_K581005
type: ComponentDefinition
persistent identity: BBa K581005
display id: BBa_K581005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc+sgrS(wt)+Terminator (small RNA regulator, conjugate part of ptsG(wt))
creator: Qin Xiao
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _752_
m user id: 0
owner id: 8589
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the conjugate part of ptsG_wt.SgrS is the wild type small RNA regulator.
mutable notes: wild type.
mutable provenance: Escherichia coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: B0015
sequence annotation: Critical region of SgrS(wt) complementary to ptsG(wt)
sequence annotation: J23106
sequence: https://synbiohub.org/public/igem/BBa_K581005_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2152357
display id: annotation2152357
version: 1
top level: BBa_K581005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K581005/annotation2152357/range2152357/1

https://synbiohub.org/public/igem/BBa_K581005/annotation2152357/range2152357/1
type: Range
persistent identity: range2152357
display id: range2152357
version: 1
direction: direction/forward
top level: BBa_K581005
start: 279
end: 407
orientation: inline

Critical region of SgrS(wt) complementary to ptsG(wt)
type: SequenceAnnotation
persistent identity: annotation2152329
display id: annotation2152329
version: 1
top level: BBa_K581005
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K581005/annotation2152329/range2152329/1

https://synbiohub.org/public/igem/BBa_K581005/annotation2152329/range2152329/1
type: Range
persistent identity: range2152329
display id: range2152329
version: 1
direction: direction/forward
top level: BBa_K581005
start: 216
end: 221
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2152328
display id: annotation2152328
version: 1
top level: BBa_K581005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K581005/annotation2152328/range2152328/1

https://synbiohub.org/public/igem/BBa_K581005/annotation2152328/range2152328/1
type: Range
persistent identity: range2152328
display id: range2152328
version: 1
direction: direction/forward
top level: BBa_K581005
start: 1
end: 35
orientation: inline

BBa_K797001
type: ComponentDefinition
persistent identity: BBa K797001
display id: BBa_K797001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phage shock protein A: one of the components of out Tat cassette
creator: Tomohiro Nobeyama
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1053_
m user id: 0
owner id: 11588
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This parts is a coding region of phage shock protein A. It ease the difference of proton potential between periplasm and cytoplasm. The expression of this protein increase the amount of secreted proteins via Tat pathway.
mutable provenance: This parts are derived from Escherichia coli's genomic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pspA
sequence: https://synbiohub.org/public/igem/BBa_K797001_sequence/1

pspA
type: SequenceAnnotation
persistent identity: annotation2195200
display id: annotation2195200
version: 1
top level: BBa_K797001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797001/annotation2195200/range2195200/1

https://synbiohub.org/public/igem/BBa_K797001/annotation2195200/range2195200/1
type: Range
persistent identity: range2195200
display id: range2195200
version: 1
direction: direction/forward
top level: BBa_K797001
start: 126
end: 794
orientation: inline

BBa_K541501
type: ComponentDefinition
persistent identity: BBa K541501
display id: BBa_K541501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Pveg, RBS spoVG and SacB signal peptide for B. subtilis
creator: Cihan TAŞTAN
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _708_
m user id: 0
owner id: 4260
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis. These parts have been taken from 2008_Imperial_Collage. Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis. SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by B. subtilis. Signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium.
mutable notes: no any design consideration
mutable provenance: All three basic components come from B.subtilis genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_K143012
sequence annotation: rbs
sequence annotation: SacB Signal tag
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence annotation: BBa_K143053
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K541501_sequence/1

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2123454
display id: annotation2123454
version: 1
top level: BBa_K541501
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123454/range2123454/1

https://synbiohub.org/public/igem/BBa_K541501/annotation2123454/range2123454/1
type: Range
persistent identity: range2123454
display id: range2123454
version: 1
direction: direction/reverse
top level: BBa_K541501
start: 1
end: 97
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2123457
display id: annotation2123457
version: 1
top level: BBa_K541501
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123457/range2123457/1

https://synbiohub.org/public/igem/BBa_K541501/annotation2123457/range2123457/1
type: Range
persistent identity: range2123457
display id: range2123457
version: 1
direction: direction/forward
top level: BBa_K541501
start: 106
end: 117
orientation: inline

SacB Signal tag
type: SequenceAnnotation
persistent identity: annotation2123460
display id: annotation2123460
version: 1
top level: BBa_K541501
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123460/range2123460/1

https://synbiohub.org/public/igem/BBa_K541501/annotation2123460/range2123460/1
type: Range
persistent identity: range2123460
display id: range2123460
version: 1
direction: direction/forward
top level: BBa_K541501
start: 124
end: 219
orientation: inline

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation2123455
display id: annotation2123455
version: 1
top level: BBa_K541501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123455/range2123455/1

https://synbiohub.org/public/igem/BBa_K541501/annotation2123455/range2123455/1
type: Range
persistent identity: range2123455
display id: range2123455
version: 1
direction: direction/forward
top level: BBa_K541501
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation2123456
display id: annotation2123456
version: 1
top level: BBa_K541501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123456/range2123456/1

https://synbiohub.org/public/igem/BBa_K541501/annotation2123456/range2123456/1
type: Range
persistent identity: range2123456
display id: range2123456
version: 1
direction: direction/forward
top level: BBa_K541501
start: 86
end: 91
orientation: inline

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2123459
display id: annotation2123459
version: 1
top level: BBa_K541501
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123459/range2123459/1

https://synbiohub.org/public/igem/BBa_K541501/annotation2123459/range2123459/1
type: Range
persistent identity: range2123459
display id: range2123459
version: 1
direction: direction/reverse
top level: BBa_K541501
start: 1
end: 117
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2123458
display id: annotation2123458
version: 1
top level: BBa_K541501
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123458/range2123458/1

https://synbiohub.org/public/igem/BBa_K541501/annotation2123458/range2123458/1
type: Range
persistent identity: range2123458
display id: range2123458
version: 1
direction: direction/reverse
top level: BBa_K541501
start: 106
end: 117
orientation: inline

BBa_K510036
type: ComponentDefinition
persistent identity: BBa K510036
display id: BBa_K510036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Improved Flip Flop (Module II: asRNA)
creator: Victor Alvarez, Felix Reyes and Yolanda Gonzalez
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6159
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Proteolysis is a very common system to regulate gene expression in all organisms. In bacteria, proteins can be targeted for proteolysis by adding a special degradation tag in their coding sequences. That???s how the Sspb-Clpx system works. In E.coli wild type, ClpX recognize specific degradation tags ??? ssrA tags -, unfolds the attached protein and translocates this denatured polypeptide into ClpP for its degradation, which is very fast. The action of this ClpXP protease is enhanced by the SspB adaptator protein. The idea is to add the DAS+4 tag to the proteins of one of the states of the flip-flop and express the Sspb under the promoter of the opposed state. Specifically, it's added the DAS+4 tag to the GFP and the LacI proteins, expressed under the cI promoter, and under the Lac promoter it's expressed Sspb. Furthermore, asRNAs are short non-coding RNAs that can inhibit mRNA translation by binding to the 5??? untranslated region (UTR) of that mRNA. That is the case of the Salmonella ompN gene, whose translation is inhibited by the conserved RybB asRNA, which forms a short duplex with the ompN CDS. This device has a fusion of OmpN start sequence with those proteins whose translation we want to regulate. The RybB asRNA binds to nucleotides from 5th to 20th relative to AUG. Consequently, a shorter fusion in which it's fused the first 7 codons of the OmpN gene CDS upstream of the RFP and Sspb CDS. '''These fusions do include the OmpN RBS'''. http://2011.igem.org/wiki/images/1/1a/UPOSevilla_finalconstruction.png Here we show the final structure of our improved bistable. As well as the two operons with their protein fusions and the promoter with the asRNA, we show the different restriction sites we have introduced and the enzymes that target each site. Finally, the total length of our construction is indicated in the figure. '''IMPORTANT''': This biobrick is '''incomplete''', you will need the asRNA device. You can find it in ... '''For further information please v…
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: PcI
sequence annotation: Terminator
sequence annotation: RybB (asRNA)
sequence: https://synbiohub.org/public/igem/BBa_K510036_sequence/1

PcI
type: SequenceAnnotation
persistent identity: annotation2135739
display id: annotation2135739
version: 1
top level: BBa_K510036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K510036/annotation2135739/range2135739/1

https://synbiohub.org/public/igem/BBa_K510036/annotation2135739/range2135739/1
type: Range
persistent identity: range2135739
display id: range2135739
version: 1
direction: direction/forward
top level: BBa_K510036
start: 1
end: 49
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2135752
display id: annotation2135752
version: 1
top level: BBa_K510036
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K510036/annotation2135752/range2135752/1

https://synbiohub.org/public/igem/BBa_K510036/annotation2135752/range2135752/1
type: Range
persistent identity: range2135752
display id: range2135752
version: 1
direction: direction/forward
top level: BBa_K510036
start: 66
end: 128
orientation: inline

RybB (asRNA)
type: SequenceAnnotation
persistent identity: annotation2135740
display id: annotation2135740
version: 1
top level: BBa_K510036
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K510036/annotation2135740/range2135740/1

https://synbiohub.org/public/igem/BBa_K510036/annotation2135740/range2135740/1
type: Range
persistent identity: range2135740
display id: range2135740
version: 1
direction: direction/forward
top level: BBa_K510036
start: 50
end: 65
orientation: inline

BBa_K539621
type: ComponentDefinition
persistent identity: BBa K539621
display id: BBa_K539621
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a RBS (B0030)+ilvC
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0030
component: BBa_K539711
sequence annotation: BBa_B0030
sequence annotation: BBa_K539711
sequence: https://synbiohub.org/public/igem/BBa_K539621_sequence/1

BBa_B0030
type: Component
persistent identity: component2143911
display id: component2143911
version: 1
top level: BBa_K539621
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K539711
type: Component
persistent identity: component2143914
display id: component2143914
version: 1
top level: BBa_K539621
access: public
definition: https://synbiohub.org/public/igem/BBa_K539711/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2143911
display id: annotation2143911
version: 1
top level: BBa_K539621
location: https://synbiohub.org/public/igem/BBa_K539621/annotation2143911/range2143911/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K539621/annotation2143911/range2143911/1
type: Range
persistent identity: range2143911
display id: range2143911
version: 1
direction: direction/forward
top level: BBa_K539621
start: 1
end: 15
orientation: inline

BBa_K539711
type: SequenceAnnotation
persistent identity: annotation2143914
display id: annotation2143914
version: 1
top level: BBa_K539621
location: https://synbiohub.org/public/igem/BBa_K539621/annotation2143914/range2143914/1
component: BBa_K539711

https://synbiohub.org/public/igem/BBa_K539621/annotation2143914/range2143914/1
type: Range
persistent identity: range2143914
display id: range2143914
version: 1
direction: direction/reverse
top level: BBa_K539621
start: 22
end: 1497
orientation: inline

BBa_K541526
type: ComponentDefinition
persistent identity: BBa K541526
display id: BBa_K541526
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: reflectin for b.subtilis with LipA signal peptide
creator: Fazilet Guler
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _708_
m user id: 0
owner id: 8463
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis. These parts have been taken from 2008_Imperial_Collage. Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis. LipA is a signal peptide from the B. subtilis genome. In general, signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached)through an appropriate secretory pathway[3]. LipA has been successfully used in the secretion of heterologous proteins such as cutinase by B. subtilis. Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli.
mutable notes: optimized for b.subtilis
mutable provenance: cephalopod and b.subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K541526_sequence/1

BBa_K331024
type: ComponentDefinition
persistent identity: BBa K331024
display id: BBa_K331024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS with N-terminal Oligo Arginine - ECFP Fusion
creator: Adam Smith
created: 2010-09-28T11:00:00Z
modified: 2015-05-08T01:12:04Z
discontinued: false
dominant: false
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has a oligo arginine sequence fused to the N-terminus of an enhanced cyan fluorescent protein. This will be a component of the proof-of-concept part BBa_K331018 which will show localization of proteins into the interior of a microcompartment formed by the assemly of lumazine synthase proteins.
mutable notes: This sequence was synthesized by Gene Art
mutable provenance: We chose not to include a transcriptional promoter to this part because we wanted the freedom to put it under the control of varied promoters. We also chose not to include a transcriptional terminator to this part because we planned to use it in an operon, where we would be expressing multiple proteins from a single transcript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K331001
sequence annotation: BBa_K331001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K331024_sequence/1

BBa_B0034
type: Component
persistent identity: component2081113
display id: component2081113
version: 1
top level: BBa_K331024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K331001
type: Component
persistent identity: component2081117
display id: component2081117
version: 1
top level: BBa_K331024
access: public
definition: https://synbiohub.org/public/igem/BBa_K331001/1

BBa_K331001
type: SequenceAnnotation
persistent identity: annotation2081117
display id: annotation2081117
version: 1
top level: BBa_K331024
location: https://synbiohub.org/public/igem/BBa_K331024/annotation2081117/range2081117/1
component: BBa_K331001

https://synbiohub.org/public/igem/BBa_K331024/annotation2081117/range2081117/1
type: Range
persistent identity: range2081117
display id: range2081117
version: 1
direction: direction/reverse
top level: BBa_K331024
start: 19
end: 780
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2081113
display id: annotation2081113
version: 1
top level: BBa_K331024
location: https://synbiohub.org/public/igem/BBa_K331024/annotation2081113/range2081113/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331024/annotation2081113/range2081113/1
type: Range
persistent identity: range2081113
display id: range2081113
version: 1
direction: direction/reverse
top level: BBa_K331024
start: 1
end: 12
orientation: inline

BBa_K577004
type: ComponentDefinition
persistent identity: BBa K577004
display id: BBa_K577004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Margaux Canevari
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
group u list: _748_
m user id: 0
owner id: 8454
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: An RFP reporter that does not glow due to the lack of the araC gene/
mutable notes: The RBS, RFP, and Terminator all come from the J61002 backbone
mutable provenance: Spring 2011 Kit Plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_B0034
component: BBa_E1010
component: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_I13453
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K577004_sequence/1

BBa_B0015
type: Component
persistent identity: component2258623
display id: component2258623
version: 1
top level: BBa_K577004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2258613
display id: component2258613
version: 1
top level: BBa_K577004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2258616
display id: component2258616
version: 1
top level: BBa_K577004
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I13453
type: Component
persistent identity: component2258611
display id: component2258611
version: 1
top level: BBa_K577004
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2258613
display id: annotation2258613
version: 1
top level: BBa_K577004
location: https://synbiohub.org/public/igem/BBa_K577004/annotation2258613/range2258613/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K577004/annotation2258613/range2258613/1
type: Range
persistent identity: range2258613
display id: range2258613
version: 1
direction: direction/reverse
top level: BBa_K577004
start: 139
end: 150
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2258611
display id: annotation2258611
version: 1
top level: BBa_K577004
location: https://synbiohub.org/public/igem/BBa_K577004/annotation2258611/range2258611/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K577004/annotation2258611/range2258611/1
type: Range
persistent identity: range2258611
display id: range2258611
version: 1
direction: direction/reverse
top level: BBa_K577004
start: 1
end: 130
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2258616
display id: annotation2258616
version: 1
top level: BBa_K577004
location: https://synbiohub.org/public/igem/BBa_K577004/annotation2258616/range2258616/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K577004/annotation2258616/range2258616/1
type: Range
persistent identity: range2258616
display id: range2258616
version: 1
direction: direction/reverse
top level: BBa_K577004
start: 157
end: 862
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2258623
display id: annotation2258623
version: 1
top level: BBa_K577004
location: https://synbiohub.org/public/igem/BBa_K577004/annotation2258623/range2258623/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K577004/annotation2258623/range2258623/1
type: Range
persistent identity: range2258623
display id: range2258623
version: 1
direction: direction/reverse
top level: BBa_K577004
start: 871
end: 999
orientation: inline

BBa_K576004
type: ComponentDefinition
persistent identity: BBa K576004
display id: BBa_K576004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right Part of Self-Excising Ribozyme
creator: George Zarubin
created: 2011-06-19T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
group u list: _747_
m user id: 0
owner id: 7330
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Modified 3' "half" of intron found in ORF #142 in Twort phage of Staphylococcus aureus in RFC53 MetaPart format. When complemented with 5' "half" BBA_K576003 in complience with BBFRFC53 assembly standard, the RNA-level excision should leave GGU linker between the whatever comes before K576003 and after K576004. K576003 and K576004 and everything in between them would be removed and possibly circularized.
mutable notes: The 3' end has been modified to accommodate MetaSuffix.
mutable provenance: [http://www.rna.whu.edu.cn/perl/gissd/get_seq_exon.cgi?intron_id=304 Group I intron Twort.v.ORF142,2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: MetaPrefix
sequence annotation: MetaSuffix
sequence annotation: IN2
sequence: https://synbiohub.org/public/igem/BBa_K576004_sequence/1

MetaPrefix
type: SequenceAnnotation
persistent identity: annotation2121635
display id: annotation2121635
version: 1
top level: BBa_K576004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K576004/annotation2121635/range2121635/1

https://synbiohub.org/public/igem/BBa_K576004/annotation2121635/range2121635/1
type: Range
persistent identity: range2121635
display id: range2121635
version: 1
direction: direction/forward
top level: BBa_K576004
start: 1
end: 10
orientation: inline

MetaSuffix
type: SequenceAnnotation
persistent identity: annotation2121637
display id: annotation2121637
version: 1
top level: BBa_K576004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K576004/annotation2121637/range2121637/1

https://synbiohub.org/public/igem/BBa_K576004/annotation2121637/range2121637/1
type: Range
persistent identity: range2121637
display id: range2121637
version: 1
direction: direction/forward
top level: BBa_K576004
start: 163
end: 172
orientation: inline

IN2
type: SequenceAnnotation
persistent identity: annotation2121636
display id: annotation2121636
version: 1
top level: BBa_K576004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K576004/annotation2121636/range2121636/1

https://synbiohub.org/public/igem/BBa_K576004/annotation2121636/range2121636/1
type: Range
persistent identity: range2121636
display id: range2121636
version: 1
direction: direction/forward
top level: BBa_K576004
start: 11
end: 162
orientation: inline

BBa_K553003
type: ComponentDefinition
persistent identity: BBa K553003
display id: BBa_K553003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive production of LasR trans-activator
creator: Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _721_
m user id: 0
owner id: 8730
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4rfr4f
mutable notes: rf23f
mutable provenance: rf243f2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_C0179
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_B0015
sequence annotation: BBa_C0179
sequence: https://synbiohub.org/public/igem/BBa_K553003_sequence/1

BBa_J23100
type: Component
persistent identity: component2130119
display id: component2130119
version: 1
top level: BBa_K553003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_C0179
type: Component
persistent identity: component2130122
display id: component2130122
version: 1
top level: BBa_K553003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_B0034
type: Component
persistent identity: component2130121
display id: component2130121
version: 1
top level: BBa_K553003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2130129
display id: component2130129
version: 1
top level: BBa_K553003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2130121
display id: annotation2130121
version: 1
top level: BBa_K553003
location: https://synbiohub.org/public/igem/BBa_K553003/annotation2130121/range2130121/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K553003/annotation2130121/range2130121/1
type: Range
persistent identity: range2130121
display id: range2130121
version: 1
direction: direction/reverse
top level: BBa_K553003
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2130119
display id: annotation2130119
version: 1
top level: BBa_K553003
location: https://synbiohub.org/public/igem/BBa_K553003/annotation2130119/range2130119/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K553003/annotation2130119/range2130119/1
type: Range
persistent identity: range2130119
display id: range2130119
version: 1
direction: direction/reverse
top level: BBa_K553003
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2130129
display id: annotation2130129
version: 1
top level: BBa_K553003
location: https://synbiohub.org/public/igem/BBa_K553003/annotation2130129/range2130129/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K553003/annotation2130129/range2130129/1
type: Range
persistent identity: range2130129
display id: range2130129
version: 1
direction: direction/reverse
top level: BBa_K553003
start: 793
end: 921
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2130122
display id: annotation2130122
version: 1
top level: BBa_K553003
location: https://synbiohub.org/public/igem/BBa_K553003/annotation2130122/range2130122/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K553003/annotation2130122/range2130122/1
type: Range
persistent identity: range2130122
display id: range2130122
version: 1
direction: direction/reverse
top level: BBa_K553003
start: 62
end: 784
orientation: inline

BBa_K608351
type: ComponentDefinition
persistent identity: BBa K608351
display id: BBa_K608351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (correct) temperature sensitive promoter
creator: Theodhor Tiko
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _780_
m user id: 0
owner id: 9113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: correct temperatursesitive promotor
mutable notes: asd
mutable provenance: asd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: OR1
sequence annotation: conserved
sequence annotation: BBa_R0051
sequence annotation: BBa_K098997
sequence annotation: stem_loop
sequence annotation: OR2
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: -35
sequence annotation: stop
sequence annotation: cIts
sequence annotation: -10
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K608351_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2131314
display id: annotation2131314
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1
type: Range
persistent identity: range2131314
display id: range2131314
version: 1
direction: direction/forward
top level: BBa_K608351
start: 924
end: 940
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131425
display id: annotation2131425
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1
type: Range
persistent identity: range2131425
display id: range2131425
version: 1
direction: direction/forward
top level: BBa_K608351
start: 776
end: 788
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131301
display id: annotation2131301
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1
type: Range
persistent identity: range2131301
display id: range2131301
version: 1
direction: direction/forward
top level: BBa_K608351
start: 48
end: 51
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2131312
display id: annotation2131312
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1
type: Range
persistent identity: range2131312
display id: range2131312
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 948
orientation: inline

BBa_K098997
type: SequenceAnnotation
persistent identity: annotation2131304
display id: annotation2131304
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1
type: Range
persistent identity: range2131304
display id: range2131304
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 62
end: 775
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2131310
display id: annotation2131310
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1
type: Range
persistent identity: range2131310
display id: range2131310
version: 1
direction: direction/forward
top level: BBa_K608351
start: 858
end: 880
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2131311
display id: annotation2131311
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1
type: Range
persistent identity: range2131311
display id: range2131311
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 916
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2131309
display id: annotation2131309
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1
type: Range
persistent identity: range2131309
display id: range2131309
version: 1
direction: direction/forward
top level: BBa_K608351
start: 846
end: 891
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2131305
display id: annotation2131305
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1
type: Range
persistent identity: range2131305
display id: range2131305
version: 1
direction: direction/forward
top level: BBa_K608351
start: 797
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2131302
display id: annotation2131302
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1
type: Range
persistent identity: range2131302
display id: range2131302
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 44
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2131313
display id: annotation2131313
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1
type: Range
persistent identity: range2131313
display id: range2131313
version: 1
direction: direction/forward
top level: BBa_K608351
start: 914
end: 919
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2131308
display id: annotation2131308
version: 1
top level: BBa_K608351
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1
type: Range
persistent identity: range2131308
display id: range2131308
version: 1
direction: direction/forward
top level: BBa_K608351
start: 830
end: 830
orientation: inline

cIts
type: SequenceAnnotation
persistent identity: annotation2131303
display id: annotation2131303
version: 1
top level: BBa_K608351
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1
type: Range
persistent identity: range2131303
display id: range2131303
version: 1
direction: direction/forward
top level: BBa_K608351
start: 62
end: 775
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2131315
display id: annotation2131315
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1
type: Range
persistent identity: range2131315
display id: range2131315
version: 1
direction: direction/forward
top level: BBa_K608351
start: 937
end: 942
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2131306
display id: annotation2131306
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1
type: Range
persistent identity: range2131306
display id: range2131306
version: 1
direction: direction/forward
top level: BBa_K608351
start: 804
end: 823
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2131307
display id: annotation2131307
version: 1
top level: BBa_K608351
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1
type: Range
persistent identity: range2131307
display id: range2131307
version: 1
direction: direction/forward
top level: BBa_K608351
start: 824
end: 837
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2131300
display id: annotation2131300
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1
type: Range
persistent identity: range2131300
display id: range2131300
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 1
end: 35
orientation: inline

tphC_322aa
type: ComponentDefinition
persistent identity: BBa K808001
display id: BBa_K808001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphC: terephtalate periplasmatic binding proteine of the tripartite transporter family
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tphC subunit of ABC tricarboxylate transporter together with the tctA and tctB subunits it forms the complet tricarboxylate transporter.
mutable notes: non
mutable provenance: Wang,Y.Z., Zhou,Y. and Zylstra,G.J., Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni YZW-D, Environ. Health Perspect. 103 SUPPL 5, 9-12 (1995) Sasoh,M., Masai,E., Ishibashi,S., Hara,H., Kamimura,N., Miyauchi,K. and Fukuda,M., Characterization of the terephthalate degradation genes of Comamonas sp. strain E6, Appl. Environ. Microbiol. 72 (3), 1825-1832 (2006)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K808001
sequence: https://synbiohub.org/public/igem/BBa_K808001_sequence/1

BBa_K808001
type: SequenceAnnotation
persistent identity: annotation2179506
display id: annotation2179506
version: 1
top level: tphC_322aa
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K808001/annotation2179506/range2179506/1

https://synbiohub.org/public/igem/BBa_K808001/annotation2179506/range2179506/1
type: Range
persistent identity: range2179506
display id: range2179506
version: 1
direction: direction/reverse
top level: tphC_322aa
start: 1
end: 969
orientation: inline

BBa_K517000
type: ComponentDefinition
persistent identity: BBa K517000
display id: BBa_K517000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GAL galactose-inducible yeast promoter
creator: Joe Ho
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _682_
m user id: 0
owner id: 9774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Promoter can be induced to express a marker gene (GFP) when exposed to galactose in the absence of glucose.
mutable notes: Not available.
mutable provenance: This promoter was taken from the S. cerevisiae Advanced Gateway Destination Vectors.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K517000_sequence/1

BBa_K608007
type: ComponentDefinition
persistent identity: BBa K608007
display id: BBa_K608007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium Promoter , weak RBS
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: medium Promotor , weak RBS
mutable notes: composite
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23110
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K608007_sequence/1

BBa_J23110
type: Component
persistent identity: component2128663
display id: component2128663
version: 1
top level: BBa_K608007
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0031
type: Component
persistent identity: component2128665
display id: component2128665
version: 1
top level: BBa_K608007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2128665
display id: annotation2128665
version: 1
top level: BBa_K608007
location: https://synbiohub.org/public/igem/BBa_K608007/annotation2128665/range2128665/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K608007/annotation2128665/range2128665/1
type: Range
persistent identity: range2128665
display id: range2128665
version: 1
direction: direction/reverse
top level: BBa_K608007
start: 44
end: 57
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2128663
display id: annotation2128663
version: 1
top level: BBa_K608007
location: https://synbiohub.org/public/igem/BBa_K608007/annotation2128663/range2128663/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K608007/annotation2128663/range2128663/1
type: Range
persistent identity: range2128663
display id: range2128663
version: 1
direction: direction/reverse
top level: BBa_K608007
start: 1
end: 35
orientation: inline

BBa_K592024
type: ComponentDefinition
persistent identity: BBa K592024
display id: BBa_K592024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-BFP
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Blue Fluorescent Protein ( BBa_K592100 ) with B0034 RBS. One can add any promoter upstream of this gene.
mutable notes: The BFP part ( BBa_K592100 ) was codon-optimized for expression in E.coli by DNA2.0
mutable provenance: The BFP part is BBa_K592100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592100
component: BBa_B0034
sequence annotation: BBa_K592100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K592024_sequence/1

BBa_K592100
type: Component
persistent identity: component2136696
display id: component2136696
version: 1
top level: BBa_K592024
access: public
definition: BFP

BBa_B0034
type: Component
persistent identity: component2136694
display id: component2136694
version: 1
top level: BBa_K592024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592100
type: SequenceAnnotation
persistent identity: annotation2136696
display id: annotation2136696
version: 1
top level: BBa_K592024
location: https://synbiohub.org/public/igem/BBa_K592024/annotation2136696/range2136696/1
component: BBa_K592100

https://synbiohub.org/public/igem/BBa_K592024/annotation2136696/range2136696/1
type: Range
persistent identity: range2136696
display id: range2136696
version: 1
direction: direction/reverse
top level: BBa_K592024
start: 19
end: 723
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136694
display id: annotation2136694
version: 1
top level: BBa_K592024
location: https://synbiohub.org/public/igem/BBa_K592024/annotation2136694/range2136694/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592024/annotation2136694/range2136694/1
type: Range
persistent identity: range2136694
display id: range2136694
version: 1
direction: direction/reverse
top level: BBa_K592024
start: 1
end: 12
orientation: inline

BBa_K624000
type: ComponentDefinition
persistent identity: BBa K624000
display id: BBa_K624000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ori+Pmsp1
creator: SHANG-JUI TSAI, CHUN-YU LAI
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _796_
m user id: 0
owner id: 6664
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: express
mutable notes: express
mutable provenance: express
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pmsp1
sequence annotation: ori (pMGT)
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K624000_sequence/1

Pmsp1
type: SequenceAnnotation
persistent identity: annotation2148113
display id: annotation2148113
version: 1
top level: BBa_K624000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K624000/annotation2148113/range2148113/1

https://synbiohub.org/public/igem/BBa_K624000/annotation2148113/range2148113/1
type: Range
persistent identity: range2148113
display id: range2148113
version: 1
direction: direction/forward
top level: BBa_K624000
start: 81
end: 274
orientation: inline

ori (pMGT)
type: SequenceAnnotation
persistent identity: annotation2148112
display id: annotation2148112
version: 1
top level: BBa_K624000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K624000/annotation2148112/range2148112/1

https://synbiohub.org/public/igem/BBa_K624000/annotation2148112/range2148112/1
type: Range
persistent identity: range2148112
display id: range2148112
version: 1
direction: direction/forward
top level: BBa_K624000
start: 1
end: 80
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148114
display id: annotation2148114
version: 1
top level: BBa_K624000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K624000/annotation2148114/range2148114/1

https://synbiohub.org/public/igem/BBa_K624000/annotation2148114/range2148114/1
type: Range
persistent identity: range2148114
display id: range2148114
version: 1
direction: direction/forward
top level: BBa_K624000
start: 275
end: 277
orientation: inline

BBa_K541502
type: ComponentDefinition
persistent identity: BBa K541502
display id: BBa_K541502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Pveg, RBS spoVG and LipA signal peptide for B. subtilis
creator: Fazilet Guler
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _708_
m user id: 0
owner id: 8463
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis. These parts have been taken from 2008_Imperial_Collage. Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis. LipA is a signal peptide from the B. subtilis genome. In general, signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached)through an appropriate secretory pathway[3]. LipA has been successfully used in the secretion of heterologous proteins such as cutinase by B. subtilis.
mutable notes: optimized for b.subtilis
mutable provenance: b.subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LipA signal peptide
sequence annotation: BBa_K143012
sequence annotation: rbs
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K541502_sequence/1

LipA signal peptide
type: SequenceAnnotation
persistent identity: annotation2123494
display id: annotation2123494
version: 1
top level: BBa_K541502
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K541502/annotation2123494/range2123494/1

https://synbiohub.org/public/igem/BBa_K541502/annotation2123494/range2123494/1
type: Range
persistent identity: range2123494
display id: range2123494
version: 1
direction: direction/forward
top level: BBa_K541502
start: 124
end: 225
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2123491
display id: annotation2123491
version: 1
top level: BBa_K541502
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K541502/annotation2123491/range2123491/1

https://synbiohub.org/public/igem/BBa_K541502/annotation2123491/range2123491/1
type: Range
persistent identity: range2123491
display id: range2123491
version: 1
direction: direction/reverse
top level: BBa_K541502
start: 1
end: 97
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2123492
display id: annotation2123492
version: 1
top level: BBa_K541502
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K541502/annotation2123492/range2123492/1

https://synbiohub.org/public/igem/BBa_K541502/annotation2123492/range2123492/1
type: Range
persistent identity: range2123492
display id: range2123492
version: 1
direction: direction/forward
top level: BBa_K541502
start: 106
end: 117
orientation: inline

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation2123489
display id: annotation2123489
version: 1
top level: BBa_K541502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K541502/annotation2123489/range2123489/1

https://synbiohub.org/public/igem/BBa_K541502/annotation2123489/range2123489/1
type: Range
persistent identity: range2123489
display id: range2123489
version: 1
direction: direction/forward
top level: BBa_K541502
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation2123490
display id: annotation2123490
version: 1
top level: BBa_K541502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K541502/annotation2123490/range2123490/1

https://synbiohub.org/public/igem/BBa_K541502/annotation2123490/range2123490/1
type: Range
persistent identity: range2123490
display id: range2123490
version: 1
direction: direction/forward
top level: BBa_K541502
start: 86
end: 91
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2123493
display id: annotation2123493
version: 1
top level: BBa_K541502
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K541502/annotation2123493/range2123493/1

https://synbiohub.org/public/igem/BBa_K541502/annotation2123493/range2123493/1
type: Range
persistent identity: range2123493
display id: range2123493
version: 1
direction: direction/reverse
top level: BBa_K541502
start: 106
end: 117
orientation: inline

BBa_K863201
type: ComponentDefinition
persistent identity: BBa K863201
display id: BBa_K863201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3' UTR site of alcohol oxidase 1 gene (aox1)
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
mutable notes: primer sequences are coming soon
mutable provenance: The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863201_sequence/1

BBa_K805015
type: ComponentDefinition
persistent identity: BBa K805015
display id: BBa_K805015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CsgD, the master regulator of biofilm formation
creator: Han Jiapeng
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1062_
m user id: 0
owner id: 11895
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: test
mutable notes: test
mutable provenance: Yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K805015_sequence/1

BBa_K584000
type: ComponentDefinition
persistent identity: BBa K584000
display id: BBa_K584000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + GFP generator
creator: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
created: 2011-08-15T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _755_
m user id: 0
owner id: 9879
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part was designed to test the functionality of the promotor region.
mutable notes: /
mutable provenance: /
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13453
component: BBa_E0240
sequence annotation: BBa_E0240
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K584000_sequence/1

BBa_I13453
type: Component
persistent identity: component2125119
display id: component2125119
version: 1
top level: BBa_K584000
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_E0240
type: Component
persistent identity: component2125130
display id: component2125130
version: 1
top level: BBa_K584000
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2125130
display id: annotation2125130
version: 1
top level: BBa_K584000
location: https://synbiohub.org/public/igem/BBa_K584000/annotation2125130/range2125130/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K584000/annotation2125130/range2125130/1
type: Range
persistent identity: range2125130
display id: range2125130
version: 1
direction: direction/reverse
top level: BBa_K584000
start: 139
end: 1014
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2125119
display id: annotation2125119
version: 1
top level: BBa_K584000
location: https://synbiohub.org/public/igem/BBa_K584000/annotation2125119/range2125119/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K584000/annotation2125119/range2125119/1
type: Range
persistent identity: range2125119
display id: range2125119
version: 1
direction: direction/reverse
top level: BBa_K584000
start: 1
end: 130
orientation: inline

BBa_K516030
type: ComponentDefinition
persistent identity: BBa K516030
display id: BBa_K516030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP protein generator TERM+ RBS+(B0030)
creator: Nicolo' Politi, Federica Sampietro, Davide Bianchini
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: mRFP protein generator TERM+ RBS+(B0030)
mutable notes: --
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0015
component: BBa_B0030
sequence annotation: BBa_B0015
sequence annotation: BBa_E1010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K516030_sequence/1

BBa_E1010
type: Component
persistent identity: component2250576
display id: component2250576
version: 1
top level: BBa_K516030
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0015
type: Component
persistent identity: component2250583
display id: component2250583
version: 1
top level: BBa_K516030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0030
type: Component
persistent identity: component2250572
display id: component2250572
version: 1
top level: BBa_K516030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250583
display id: annotation2250583
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250583/range2250583/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516030/annotation2250583/range2250583/1
type: Range
persistent identity: range2250583
display id: range2250583
version: 1
direction: direction/reverse
top level: BBa_K516030
start: 736
end: 864
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250576
display id: annotation2250576
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250576/range2250576/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K516030/annotation2250576/range2250576/1
type: Range
persistent identity: range2250576
display id: range2250576
version: 1
direction: direction/reverse
top level: BBa_K516030
start: 22
end: 727
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2250572
display id: annotation2250572
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250572/range2250572/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K516030/annotation2250572/range2250572/1
type: Range
persistent identity: range2250572
display id: range2250572
version: 1
direction: direction/forward
top level: BBa_K516030
start: 1
end: 15
orientation: inline

BBa_K624001
type: ComponentDefinition
persistent identity: BBa K624001
display id: BBa_K624001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR+RBS+GFP+ter
creator: SHIH- HAO CHEN
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _796_
m user id: 0
owner id: 6664
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Add a promoter and a RBS on the upstream will express tetR protein and GFP. GFP can help detecting tetR expression.
mutable notes: no
mutable provenance: Biobrick parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_E0840
component: BBa_C0040
sequence annotation: BBa_C0040
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K624001_sequence/1

BBa_E0840
type: Component
persistent identity: component2258953
display id: component2258953
version: 1
top level: BBa_K624001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_C0040
type: Component
persistent identity: component2258942
display id: component2258942
version: 1
top level: BBa_K624001
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2258942
display id: annotation2258942
version: 1
top level: BBa_K624001
location: https://synbiohub.org/public/igem/BBa_K624001/annotation2258942/range2258942/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K624001/annotation2258942/range2258942/1
type: Range
persistent identity: range2258942
display id: range2258942
version: 1
direction: direction/reverse
top level: BBa_K624001
start: 1
end: 685
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2258953
display id: annotation2258953
version: 1
top level: BBa_K624001
location: https://synbiohub.org/public/igem/BBa_K624001/annotation2258953/range2258953/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K624001/annotation2258953/range2258953/1
type: Range
persistent identity: range2258953
display id: range2258953
version: 1
direction: direction/reverse
top level: BBa_K624001
start: 694
end: 1571
orientation: inline

BBa_K568001
type: ComponentDefinition
persistent identity: BBa K568001
display id: BBa_K568001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Optogenetical AND-Gate red/blue light
creator: Team TU Munich 2011
created: 2011-08-31T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _736_
m user id: 0
owner id: 9896
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD. The blue light sensor induces the expression of a mRNA coding for a T7-polymerase with an amber mutation, that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor is induced. Thus, only cells which receive both signals produce the desired substances.
mutable notes: We used YcgF/E as blue light sensor and Cph8 as red light sensor. We expect that this assembly will be the one with the lowest amount of unspecific gene expression which could occur due to overlapping of the absorption spectrums of the sensory domains.
mutable provenance: designed from existing Biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K322123
component: BBa_K238013
component: BBa_K228001
component: BBa_K228000
component: BBa_K322124
component: BBa_J44001
component: BBa_R0082
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_J44001
sequence annotation: BBa_R0082
sequence annotation: BBa_K322123
sequence annotation: BBa_K238013
sequence annotation: BBa_K322124
sequence annotation: BBa_K228000
sequence annotation: BBa_K228001
sequence: https://synbiohub.org/public/igem/BBa_K568001_sequence/1

BBa_K322123
type: Component
persistent identity: component2125718
display id: component2125718
version: 1
top level: BBa_K568001
access: public
definition: https://synbiohub.org/public/igem/BBa_K322123/1

BBa_K238013
type: Component
persistent identity: component2125742
display id: component2125742
version: 1
top level: BBa_K568001
access: public
definition: https://synbiohub.org/public/igem/BBa_K238013/1

BBa_K228001
type: Component
persistent identity: component2125728
display id: component2125728
version: 1
top level: BBa_K568001
access: public
definition: https://synbiohub.org/public/igem/BBa_K228001/1

BBa_K228000
type: Component
persistent identity: component2125746
display id: component2125746
version: 1
top level: BBa_K568001
access: public
definition: https://synbiohub.org/public/igem/BBa_K228000/1

BBa_K322124
type: Component
persistent identity: component2125721
display id: component2125721
version: 1
top level: BBa_K568001
access: public
definition: https://synbiohub.org/public/igem/BBa_K322124/1

BBa_J44001
type: Component
persistent identity: component2125744
display id: component2125744
version: 1
top level: BBa_K568001
access: public
definition: https://synbiohub.org/public/igem/BBa_J44001/1

BBa_R0082
type: Component
persistent identity: component2125727
display id: component2125727
version: 1
top level: BBa_K568001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0015
type: Component
persistent identity: component2125735
display id: component2125735
version: 1
top level: BBa_K568001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2125735
display id: annotation2125735
version: 1
top level: BBa_K568001
location: https://synbiohub.org/public/igem/BBa_K568001/annotation2125735/range2125735/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K568001/annotation2125735/range2125735/1
type: Range
persistent identity: range2125735
display id: range2125735
version: 1
direction: direction/reverse
top level: BBa_K568001
start: 3929
end: 4057
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2125744
display id: annotation2125744
version: 1
top level: BBa_K568001
location: https://synbiohub.org/public/igem/BBa_K568001/annotation2125744/range2125744/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_K568001/annotation2125744/range2125744/1
type: Range
persistent identity: range2125744
display id: range2125744
version: 1
direction: direction/reverse
top level: BBa_K568001
start: 4160
end: 4174
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2125727
display id: annotation2125727
version: 1
top level: BBa_K568001
location: https://synbiohub.org/public/igem/BBa_K568001/annotation2125727/range2125727/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K568001/annotation2125727/range2125727/1
type: Range
persistent identity: range2125727
display id: range2125727
version: 1
direction: direction/reverse
top level: BBa_K568001
start: 3669
end: 3776
orientation: inline

BBa_K322123
type: SequenceAnnotation
persistent identity: annotation2125718
display id: annotation2125718
version: 1
top level: BBa_K568001
location: https://synbiohub.org/public/igem/BBa_K568001/annotation2125718/range2125718/1
component: BBa_K322123

https://synbiohub.org/public/igem/BBa_K568001/annotation2125718/range2125718/1
type: Range
persistent identity: range2125718
display id: range2125718
version: 1
direction: direction/reverse
top level: BBa_K568001
start: 1
end: 1399
orientation: inline

BBa_K238013
type: SequenceAnnotation
persistent identity: annotation2125742
display id: annotation2125742
version: 1
top level: BBa_K568001
location: https://synbiohub.org/public/igem/BBa_K568001/annotation2125742/range2125742/1
component: BBa_K238013

https://synbiohub.org/public/igem/BBa_K568001/annotation2125742/range2125742/1
type: Range
persistent identity: range2125742
display id: range2125742
version: 1
direction: direction/reverse
top level: BBa_K568001
start: 4066
end: 4151
orientation: inline

BBa_K322124
type: SequenceAnnotation
persistent identity: annotation2125721
display id: annotation2125721
version: 1
top level: BBa_K568001
location: https://synbiohub.org/public/igem/BBa_K568001/annotation2125721/range2125721/1
component: BBa_K322124

https://synbiohub.org/public/igem/BBa_K568001/annotation2125721/range2125721/1
type: Range
persistent identity: range2125721
display id: range2125721
version: 1
direction: direction/reverse
top level: BBa_K568001
start: 1408
end: 3660
orientation: inline

BBa_K228000
type: SequenceAnnotation
persistent identity: annotation2125746
display id: annotation2125746
version: 1
top level: BBa_K568001
location: https://synbiohub.org/public/igem/BBa_K568001/annotation2125746/range2125746/1
component: BBa_K228000

https://synbiohub.org/public/igem/BBa_K568001/annotation2125746/range2125746/1
type: Range
persistent identity: range2125746
display id: range2125746
version: 1
direction: direction/reverse
top level: BBa_K568001
start: 4181
end: 6853
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2125728
display id: annotation2125728
version: 1
top level: BBa_K568001
location: https://synbiohub.org/public/igem/BBa_K568001/annotation2125728/range2125728/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K568001/annotation2125728/range2125728/1
type: Range
persistent identity: range2125728
display id: range2125728
version: 1
direction: direction/reverse
top level: BBa_K568001
start: 3785
end: 3920
orientation: inline

BBa_K517003
type: ComponentDefinition
persistent identity: BBa K517003
display id: BBa_K517003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: beta-pinene synthase
creator: Marianne Park
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _682_
m user id: 0
owner id: 9774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The (-)-b-pinene synthase (PsTPS-Pin2) synthase is a monoterpene synthase isolated from Sitka spruce converts geranyl pyrophosphate to 70.5%(-)-beta-pinene, 29.5%(-)-alpha-pinene.
mutable notes: none available
mutable provenance: The (-)-b-pinene synthase (PsTPS-Pin2) synthase is a monoterpene synthase isolated from Sitka spruce.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K517003_sequence/1

BBa_K543020
type: ComponentDefinition
persistent identity: BBa K543020
display id: BBa_K543020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriTr-GFP
creator: Yuki Ito
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _710_
m user id: 0
owner id: 5966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OriTr(BBa_J01003)+GFP(BBa_I13504).
mutable notes: For checking conjugation
mutable provenance: OriTr(BBa_J01003) GFP(BBa_I13504)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
component: BBa_I13504
component: BBa_J01003
sequence annotation: BBa_I13504
sequence annotation: BBa_J01003
sequence: https://synbiohub.org/public/igem/BBa_K543020_sequence/1

BBa_I13504
type: Component
persistent identity: component2143828
display id: component2143828
version: 1
top level: BBa_K543020
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_J01003
type: Component
persistent identity: component2143818
display id: component2143818
version: 1
top level: BBa_K543020
access: public
definition: https://synbiohub.org/public/igem/BBa_J01003/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2143828
display id: annotation2143828
version: 1
top level: BBa_K543020
location: https://synbiohub.org/public/igem/BBa_K543020/annotation2143828/range2143828/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K543020/annotation2143828/range2143828/1
type: Range
persistent identity: range2143828
display id: range2143828
version: 1
direction: direction/reverse
top level: BBa_K543020
start: 380
end: 1254
orientation: inline

BBa_J01003
type: SequenceAnnotation
persistent identity: annotation2143818
display id: annotation2143818
version: 1
top level: BBa_K543020
location: https://synbiohub.org/public/igem/BBa_K543020/annotation2143818/range2143818/1
component: BBa_J01003

https://synbiohub.org/public/igem/BBa_K543020/annotation2143818/range2143818/1
type: Range
persistent identity: range2143818
display id: range2143818
version: 1
direction: direction/reverse
top level: BBa_K543020
start: 1
end: 371
orientation: inline

BBa_K914011
type: ComponentDefinition
persistent identity: BBa K914011
display id: BBa_K914011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: supD-T - intermediate
creator: Jean Cury
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the intermediate of the K914000 part, so you can change the promoter easily.
mutable notes: The terminator is mutated, but it does not impact the function.
mutable provenance: made from 2 biobricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: modified thr terminator
sequence annotation: B1006
sequence annotation: BBa_K228001
sequence: https://synbiohub.org/public/igem/BBa_K914011_sequence/1

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation2189725
display id: annotation2189725
version: 1
top level: BBa_K914011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K914011/annotation2189725/range2189725/1

https://synbiohub.org/public/igem/BBa_K914011/annotation2189725/range2189725/1
type: Range
persistent identity: range2189725
display id: range2189725
version: 1
direction: direction/forward
top level: BBa_K914011
start: 154
end: 175
orientation: inline

B1006
type: SequenceAnnotation
persistent identity: annotation2189724
display id: annotation2189724
version: 1
top level: BBa_K914011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914011/annotation2189724/range2189724/1

https://synbiohub.org/public/igem/BBa_K914011/annotation2189724/range2189724/1
type: Range
persistent identity: range2189724
display id: range2189724
version: 1
direction: direction/forward
top level: BBa_K914011
start: 145
end: 183
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2189723
display id: annotation2189723
version: 1
top level: BBa_K914011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914011/annotation2189723/range2189723/1

https://synbiohub.org/public/igem/BBa_K914011/annotation2189723/range2189723/1
type: Range
persistent identity: range2189723
display id: range2189723
version: 1
direction: direction/reverse
top level: BBa_K914011
start: 1
end: 136
orientation: inline

BBa_K523006
type: ComponentDefinition
persistent identity: BBa K523006
display id: BBa_K523006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac + LacZ + malS
creator: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman
created: 2011-07-28T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: ''E. coli'' amylase gene ''malS'' under control of lac promoter. LacZ is also present for blue/white screening. This contains the native ''malS'' RBS.
mutable notes: N/A
mutable provenance: BBa_J33207 + BBa_K523001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K523001
component: BBa_J33207
sequence annotation: BBa_K523001
sequence annotation: BBa_J33207
sequence: https://synbiohub.org/public/igem/BBa_K523006_sequence/1

BBa_K523001
type: Component
persistent identity: component2124213
display id: component2124213
version: 1
top level: BBa_K523006
access: public
definition: BBa_K523001

BBa_J33207
type: Component
persistent identity: component2124206
display id: component2124206
version: 1
top level: BBa_K523006
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_K523001
type: SequenceAnnotation
persistent identity: annotation2124213
display id: annotation2124213
version: 1
top level: BBa_K523006
location: https://synbiohub.org/public/igem/BBa_K523006/annotation2124213/range2124213/1
component: BBa_K523001

https://synbiohub.org/public/igem/BBa_K523006/annotation2124213/range2124213/1
type: Range
persistent identity: range2124213
display id: range2124213
version: 1
direction: direction/reverse
top level: BBa_K523006
start: 609
end: 2675
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2124206
display id: annotation2124206
version: 1
top level: BBa_K523006
location: https://synbiohub.org/public/igem/BBa_K523006/annotation2124206/range2124206/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K523006/annotation2124206/range2124206/1
type: Range
persistent identity: range2124206
display id: range2124206
version: 1
direction: direction/reverse
top level: BBa_K523006
start: 1
end: 600
orientation: inline

BBa_K863206
type: ComponentDefinition
persistent identity: BBa K863206
display id: BBa_K863206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mating factor alpha 1 with Kozak sequence
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: long description is coming soon
mutable notes: design considerations are coming soon
mutable provenance: The Kozak sequence is based on the BioBrick BBa_J63003, but the sequence is used without the last bases atggag. And the MFalpha1 sequence is from the plasmid pPICZalpha A from Invitrogen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863206_sequence/1

BBa_K873002
type: ComponentDefinition
persistent identity: BBa K873002
display id: BBa_K873002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HSP promoter
creator: yue hu
created: 2012-09-17T11:00:00Z
modified: 2016-10-16T09:49:24Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1134_
m user id: 29300
owner id: 11547
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a heat shock promoter, it refers to the transcription factor sigma32. when the temperature rise, the transcription factor sigma32 expresses a lot and binds to the RNA ploymerase to recognize the conserved sequence in -35 and -10 region, then the promoter starts to transcript.
mutable notes: The whole sequence of this part is only 90bp, so take a futher consideration if you intend to digest it for use.
mutable provenance: We align the HSP promoters from the literature and then find the conserved sequence, then we just modified the promoter of groES of E.coli, and synthesis the part through BIC genescript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K873002_sequence/1

BBa_K517001
type: ComponentDefinition
persistent identity: BBa K517001
display id: BBa_K517001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GPD constitutive yeast promoter
creator: Joe Ho
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _682_
m user id: 0
owner id: 9774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Promoter for constitutively high expression.
mutable notes: Not available
mutable provenance: This promoter was taken from the S. cerevisiae Advanced Gateway Destination Vectors.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K517001_sequence/1

BBa_K617004
type: ComponentDefinition
persistent identity: BBa K617004
display id: BBa_K617004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda attP P'OP
creator: Will Jones
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _789_
m user id: 0
owner id: 4301
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lambda phage attachement site
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: P'
sequence annotation: O
sequence annotation: P
sequence: https://synbiohub.org/public/igem/BBa_K617004_sequence/1

P'
type: SequenceAnnotation
persistent identity: annotation2146519
display id: annotation2146519
version: 1
top level: BBa_K617004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K617004/annotation2146519/range2146519/1

https://synbiohub.org/public/igem/BBa_K617004/annotation2146519/range2146519/1
type: Range
persistent identity: range2146519
display id: range2146519
version: 1
direction: direction/forward
top level: BBa_K617004
start: 1
end: 246
orientation: inline

O
type: SequenceAnnotation
persistent identity: annotation2146520
display id: annotation2146520
version: 1
top level: BBa_K617004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K617004/annotation2146520/range2146520/1

https://synbiohub.org/public/igem/BBa_K617004/annotation2146520/range2146520/1
type: Range
persistent identity: range2146520
display id: range2146520
version: 1
direction: direction/forward
top level: BBa_K617004
start: 246
end: 267
orientation: inline

P
type: SequenceAnnotation
persistent identity: annotation2146516
display id: annotation2146516
version: 1
top level: BBa_K617004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K617004/annotation2146516/range2146516/1

https://synbiohub.org/public/igem/BBa_K617004/annotation2146516/range2146516/1
type: Range
persistent identity: range2146516
display id: range2146516
version: 1
direction: direction/forward
top level: BBa_K617004
start: 267
end: 396
orientation: inline

BBa_K316027
type: ComponentDefinition
persistent identity: BBa K316027
display id: BBa_K316027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B.subtilis transformation vector with LacI
creator: IC 2010 Team
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: yey
mutable notes: This part is designed for integration into ''B. subtilis' genome at amyE locus, it contains Spectinomycin resistance selection marker and constitutively expresses LacI. PmeI can be used to ligate a gene of interest using blunt ended methods.
mutable provenance: Existing biobricks, BBa_K143070 , BBa_K316002 , BBa_K316014 BBa_K143002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143001
component: BBa_K143065
component: BBa_K143053
component: BBa_K316014
component: BBa_K143002
component: BBa_K143062
sequence annotation: BBa_K143001
sequence annotation: BBa_K143062
sequence annotation: BBa_K143002
sequence annotation: BBa_K143053
sequence annotation: BBa_K143065
sequence annotation: BBa_K316014
sequence: https://synbiohub.org/public/igem/BBa_K316027_sequence/1

BBa_K143001
type: Component
persistent identity: component2102333
display id: component2102333
version: 1
top level: BBa_K316027
access: public
definition: https://synbiohub.org/public/igem/BBa_K143001/1

BBa_K143065
type: Component
persistent identity: component2102348
display id: component2102348
version: 1
top level: BBa_K316027
access: public
definition: https://synbiohub.org/public/igem/BBa_K143065/1

BBa_K143053
type: Component
persistent identity: component2102354
display id: component2102354
version: 1
top level: BBa_K316027
access: public
definition: https://synbiohub.org/public/igem/BBa_K143053/1

BBa_K316014
type: Component
persistent identity: component2102371
display id: component2102371
version: 1
top level: BBa_K316027
access: public
definition: https://synbiohub.org/public/igem/BBa_K316014/1

BBa_K143002
type: Component
persistent identity: component2102373
display id: component2102373
version: 1
top level: BBa_K316027
access: public
definition: https://synbiohub.org/public/igem/BBa_K143002/1

BBa_K143062
type: Component
persistent identity: component2102366
display id: component2102366
version: 1
top level: BBa_K316027
access: public
definition: https://synbiohub.org/public/igem/BBa_K143062/1

BBa_K143053
type: Component
persistent identity: component2102339
display id: component2102339
version: 1
top level: BBa_K316027
access: public
definition: https://synbiohub.org/public/igem/BBa_K143053/1

BBa_K143001
type: SequenceAnnotation
persistent identity: annotation2102333
display id: annotation2102333
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102333/range2102333/1
component: BBa_K143001

https://synbiohub.org/public/igem/BBa_K316027/annotation2102333/range2102333/1
type: Range
persistent identity: range2102333
display id: range2102333
version: 1
direction: direction/reverse
top level: BBa_K316027
start: 1
end: 522
orientation: inline

BBa_K143062
type: SequenceAnnotation
persistent identity: annotation2102366
display id: annotation2102366
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102366/range2102366/1
component: BBa_K143062

https://synbiohub.org/public/igem/BBa_K316027/annotation2102366/range2102366/1
type: Range
persistent identity: range2102366
display id: range2102366
version: 1
direction: direction/reverse
top level: BBa_K316027
start: 1693
end: 2915
orientation: inline

BBa_K143002
type: SequenceAnnotation
persistent identity: annotation2102373
display id: annotation2102373
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102373/range2102373/1
component: BBa_K143002

https://synbiohub.org/public/igem/BBa_K316027/annotation2102373/range2102373/1
type: Range
persistent identity: range2102373
display id: range2102373
version: 1
direction: direction/reverse
top level: BBa_K316027
start: 2976
end: 3977
orientation: inline

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2102339
display id: annotation2102339
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102339/range2102339/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K316027/annotation2102339/range2102339/1
type: Range
persistent identity: range2102339
display id: range2102339
version: 1
direction: direction/reverse
top level: BBa_K316027
start: 531
end: 647
orientation: inline

BBa_K143065
type: SequenceAnnotation
persistent identity: annotation2102348
display id: annotation2102348
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102348/range2102348/1
component: BBa_K143065

https://synbiohub.org/public/igem/BBa_K316027/annotation2102348/range2102348/1
type: Range
persistent identity: range2102348
display id: range2102348
version: 1
direction: direction/reverse
top level: BBa_K316027
start: 654
end: 1561
orientation: inline

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2102354
display id: annotation2102354
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102354/range2102354/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K316027/annotation2102354/range2102354/1
type: Range
persistent identity: range2102354
display id: range2102354
version: 1
direction: direction/reverse
top level: BBa_K316027
start: 1570
end: 1686
orientation: inline

BBa_K316014
type: SequenceAnnotation
persistent identity: annotation2102371
display id: annotation2102371
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102371/range2102371/1
component: BBa_K316014

https://synbiohub.org/public/igem/BBa_K316027/annotation2102371/range2102371/1
type: Range
persistent identity: range2102371
display id: range2102371
version: 1
direction: direction/reverse
top level: BBa_K316027
start: 2924
end: 2967
orientation: inline

BBa_K823007
type: ComponentDefinition
persistent identity: BBa K823007
display id: BBa_K823007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23103
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:46:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23103 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: J23103
sequence: https://synbiohub.org/public/igem/BBa_K823007_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182517
display id: annotation2182517
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1
type: Range
persistent identity: range2182517
display id: range2182517
version: 1
direction: direction/forward
top level: BBa_K823007
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182516
display id: annotation2182516
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1
type: Range
persistent identity: range2182516
display id: range2182516
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 6
orientation: inline

J23103
type: SequenceAnnotation
persistent identity: annotation2182515
display id: annotation2182515
version: 1
top level: BBa_K823007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1
type: Range
persistent identity: range2182515
display id: range2182515
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 35
orientation: inline

BBa_K592002
type: ComponentDefinition
persistent identity: BBa K592002
display id: BBa_K592002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ccaR
creator: Erik Lundin
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ccaR is the response regulator of the green sensor that also includes the membrane-associated histidine kinase ccaS. The two-componet system is also dependent of a chromophore.
mutable notes: The ccaR amplified from pJT118 was point mutated to get rid of illegal restriction site.
mutable provenance: The ccaR was amplified from a pJT118 provided by Voight et al (2011) Multichromatic Control of Gene Expression in Escherichia coli, J. Mol. Biol., 405, p315???324
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K592002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2136237
display id: annotation2136237
version: 1
top level: BBa_K592002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592002/annotation2136237/range2136237/1

https://synbiohub.org/public/igem/BBa_K592002/annotation2136237/range2136237/1
type: Range
persistent identity: range2136237
display id: range2136237
version: 1
direction: direction/forward
top level: BBa_K592002
start: 1
end: 708
orientation: inline

BBa_K656010
type: ComponentDefinition
persistent identity: BBa K656010
display id: BBa_K656010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anabaena pSac Promoter
creator: Evan Clark, Julius Ho, Eli Moss, Jesse Palmer
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _833_
m user id: 0
owner id: 9641
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anabaena vegetative cell constitutive promoter. Can be used to express specifically in vegetative cells, excluding heterocyst expression.
mutable provenance: Anabaena Variabilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K656010_sequence/1

BBa_K733001
type: ComponentDefinition
persistent identity: BBa K733001
display id: BBa_K733001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptms - a constitutive promoter in E. coli and B. subtilis.
creator: GU, Bida; SUN Fei
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A binding sequence, -35
sequence annotation: Sigma A binding sequence, -10
sequence: https://synbiohub.org/public/igem/BBa_K733001_sequence/1

Sigma A binding sequence, -35
type: SequenceAnnotation
persistent identity: annotation2193526
display id: annotation2193526
version: 1
top level: BBa_K733001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K733001/annotation2193526/range2193526/1

https://synbiohub.org/public/igem/BBa_K733001/annotation2193526/range2193526/1
type: Range
persistent identity: range2193526
display id: range2193526
version: 1
direction: direction/forward
top level: BBa_K733001
start: 14
end: 20
orientation: inline

Sigma A binding sequence, -10
type: SequenceAnnotation
persistent identity: annotation2193570
display id: annotation2193570
version: 1
top level: BBa_K733001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K733001/annotation2193570/range2193570/1

https://synbiohub.org/public/igem/BBa_K733001/annotation2193570/range2193570/1
type: Range
persistent identity: range2193570
display id: range2193570
version: 1
direction: direction/forward
top level: BBa_K733001
start: 40
end: 46
orientation: inline

BBa_K314103
type: ComponentDefinition
persistent identity: BBa K314103
display id: BBa_K314103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac induced expression cassette
creator: Tomas Huber, Justin Siegel
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lac induced protein expression insert includes f1 origin, Lac I gene and RBS
mutable provenance: construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_B0034
component: BBa_K314110
component: BBa_K314111
sequence annotation: BBa_B0034
sequence annotation: BBa_K314110
sequence annotation: BBa_K314111
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K314103_sequence/1

BBa_R0011
type: Component
persistent identity: component2091083
display id: component2091083
version: 1
top level: BBa_K314103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component2091089
display id: component2091089
version: 1
top level: BBa_K314103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K314110
type: Component
persistent identity: component2091081
display id: component2091081
version: 1
top level: BBa_K314103
access: public
definition: f1 ori

BBa_K314111
type: Component
persistent identity: component2091082
display id: component2091082
version: 1
top level: BBa_K314103
access: public
definition: https://synbiohub.org/public/igem/BBa_K314111/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2091089
display id: annotation2091089
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091089/range2091089/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K314103/annotation2091089/range2091089/1
type: Range
persistent identity: range2091089
display id: range2091089
version: 1
direction: direction/reverse
top level: BBa_K314103
start: 1627
end: 1638
orientation: inline

BBa_K314110
type: SequenceAnnotation
persistent identity: annotation2091081
display id: annotation2091081
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091081/range2091081/1
component: BBa_K314110

https://synbiohub.org/public/igem/BBa_K314103/annotation2091081/range2091081/1
type: Range
persistent identity: range2091081
display id: range2091081
version: 1
direction: direction/reverse
top level: BBa_K314103
start: 1
end: 455
orientation: inline

BBa_K314111
type: SequenceAnnotation
persistent identity: annotation2091082
display id: annotation2091082
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091082/range2091082/1
component: BBa_K314111

https://synbiohub.org/public/igem/BBa_K314103/annotation2091082/range2091082/1
type: Range
persistent identity: range2091082
display id: range2091082
version: 1
direction: direction/reverse
top level: BBa_K314103
start: 464
end: 1555
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2091083
display id: annotation2091083
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091083/range2091083/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K314103/annotation2091083/range2091083/1
type: Range
persistent identity: range2091083
display id: range2091083
version: 1
direction: direction/forward
top level: BBa_K314103
start: 1564
end: 1617
orientation: inline

BBa_K808011
type: ComponentDefinition
persistent identity: BBa K808011
display id: BBa_K808011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphA1: reduces the tphA2A3 complex
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808011_sequence/1

BBa_K817002
type: ComponentDefinition
persistent identity: BBa K817002
display id: BBa_K817002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PfadBA
creator: Yi-Te Lee, Chieh-Mei Wang
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1075_
m user id: 0
owner id: 13572
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fatty acid sensitive promoter
mutable notes: N/A
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PfadBA
sequence: https://synbiohub.org/public/igem/BBa_K817002_sequence/1

PfadBA
type: SequenceAnnotation
persistent identity: annotation2197686
display id: annotation2197686
version: 1
top level: BBa_K817002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K817002/annotation2197686/range2197686/1

https://synbiohub.org/public/igem/BBa_K817002/annotation2197686/range2197686/1
type: Range
persistent identity: range2197686
display id: range2197686
version: 1
direction: direction/forward
top level: BBa_K817002
start: 1
end: 72
orientation: inline

BBa_K539742
type: ComponentDefinition
persistent identity: BBa K539742
display id: BBa_K539742
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_B0015)
creator: Yang, Yi-Pei
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of kivd from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This is part of the isobutanol biosynthesis pathway. This part kivd is under TetR repressible promoter that constitutively ON and repressed by TetRcan. And terminator BBa_B0015 is included.
mutable notes: It is a working biobrick.
mutable provenance: It is cloned form Lactococcus lactis subsp. lactis KF147
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K539714
component: BBa_R0040
component: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_K539714
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K539742_sequence/1

BBa_B0034
type: Component
persistent identity: component2222929
display id: component2222929
version: 1
top level: BBa_K539742
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K539714
type: Component
persistent identity: component2222931
display id: component2222931
version: 1
top level: BBa_K539742
access: public
definition: https://synbiohub.org/public/igem/BBa_K539714/1

BBa_R0040
type: Component
persistent identity: component2222923
display id: component2222923
version: 1
top level: BBa_K539742
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2222938
display id: component2222938
version: 1
top level: BBa_K539742
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2222923
display id: annotation2222923
version: 1
top level: BBa_K539742
location: https://synbiohub.org/public/igem/BBa_K539742/annotation2222923/range2222923/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K539742/annotation2222923/range2222923/1
type: Range
persistent identity: range2222923
display id: range2222923
version: 1
direction: direction/forward
top level: BBa_K539742
start: 1
end: 54
orientation: inline

BBa_K539714
type: SequenceAnnotation
persistent identity: annotation2222931
display id: annotation2222931
version: 1
top level: BBa_K539742
location: https://synbiohub.org/public/igem/BBa_K539742/annotation2222931/range2222931/1
component: BBa_K539714

https://synbiohub.org/public/igem/BBa_K539742/annotation2222931/range2222931/1
type: Range
persistent identity: range2222931
display id: range2222931
version: 1
direction: direction/reverse
top level: BBa_K539742
start: 81
end: 1727
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222929
display id: annotation2222929
version: 1
top level: BBa_K539742
location: https://synbiohub.org/public/igem/BBa_K539742/annotation2222929/range2222929/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K539742/annotation2222929/range2222929/1
type: Range
persistent identity: range2222929
display id: range2222929
version: 1
direction: direction/reverse
top level: BBa_K539742
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2222938
display id: annotation2222938
version: 1
top level: BBa_K539742
location: https://synbiohub.org/public/igem/BBa_K539742/annotation2222938/range2222938/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K539742/annotation2222938/range2222938/1
type: Range
persistent identity: range2222938
display id: range2222938
version: 1
direction: direction/reverse
top level: BBa_K539742
start: 1736
end: 1864
orientation: inline

BBa_K741003
type: ComponentDefinition
persistent identity: BBa K741003
display id: BBa_K741003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRM-RBS-GFP-T
creator: Wuyang Chen
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Express GFP only if protein CI activated the promoter pRM.
mutable notes: TBD
mutable provenance: BBa_I13504 BBa_I12007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I12007
component: BBa_I13504
sequence annotation: BBa_I12007
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K741003_sequence/1

BBa_I12007
type: Component
persistent identity: component2177932
display id: component2177932
version: 1
top level: BBa_K741003
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_I13504
type: Component
persistent identity: component2177942
display id: component2177942
version: 1
top level: BBa_K741003
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation2177932
display id: annotation2177932
version: 1
top level: BBa_K741003
location: https://synbiohub.org/public/igem/BBa_K741003/annotation2177932/range2177932/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_K741003/annotation2177932/range2177932/1
type: Range
persistent identity: range2177932
display id: range2177932
version: 1
direction: direction/reverse
top level: BBa_K741003
start: 1
end: 82
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2177942
display id: annotation2177942
version: 1
top level: BBa_K741003
location: https://synbiohub.org/public/igem/BBa_K741003/annotation2177942/range2177942/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K741003/annotation2177942/range2177942/1
type: Range
persistent identity: range2177942
display id: range2177942
version: 1
direction: direction/reverse
top level: BBa_K741003
start: 91
end: 965
orientation: inline

BBa_K895006
type: ComponentDefinition
persistent identity: BBa K895006
display id: BBa_K895006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP open reading frame
creator: Frank Sargent
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:41Z
discontinued: false
dominant: false
group u list: _1160_
m user id: 0
owner id: 8083
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is GFP from Biobrick BBa_J54103 , which we have sequenced and re-cloned to RFC[10] standard in pSB1C3.
mutable notes: None
mutable provenance: Amplified by PCR from BBa_J54103
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K895006_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2194605
display id: annotation2194605
version: 1
top level: BBa_K895006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K895006/annotation2194605/range2194605/1

https://synbiohub.org/public/igem/BBa_K895006/annotation2194605/range2194605/1
type: Range
persistent identity: range2194605
display id: range2194605
version: 1
direction: direction/forward
top level: BBa_K895006
start: 1
end: 720
orientation: inline

BBa_K864102
type: ComponentDefinition
persistent identity: BBa K864102
display id: BBa_K864102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-SYFP2
creator: Arvid Hed??n Gynn??
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 13993
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the Super Yellow Fluorescent Protein 2 (SYFP2) ( BBa_K864100 ) with the strong BBa_B0034 RBS. ===Usage and Biology=== This part is usable as a reporter. The very strong fluorescence means transcription can be detected at low levels. Colonies are very clearly seen on a UV table with most promoters.
mutable notes: Transcriptional leakage is present in some backbones, giving visibly fluorescent colonies even without promoter.
mutable provenance: Assembly of K864100 and the B0034 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K864100
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K864100
sequence: https://synbiohub.org/public/igem/BBa_K864102_sequence/1

BBa_K864100
type: Component
persistent identity: component2197168
display id: component2197168
version: 1
top level: BBa_K864102
access: public
definition: SYFP2

BBa_B0034
type: Component
persistent identity: component2197166
display id: component2197166
version: 1
top level: BBa_K864102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2197166
display id: annotation2197166
version: 1
top level: BBa_K864102
location: https://synbiohub.org/public/igem/BBa_K864102/annotation2197166/range2197166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K864102/annotation2197166/range2197166/1
type: Range
persistent identity: range2197166
display id: range2197166
version: 1
direction: direction/reverse
top level: BBa_K864102
start: 1
end: 12
orientation: inline

BBa_K864100
type: SequenceAnnotation
persistent identity: annotation2197168
display id: annotation2197168
version: 1
top level: BBa_K864102
location: https://synbiohub.org/public/igem/BBa_K864102/annotation2197168/range2197168/1
component: BBa_K864100

https://synbiohub.org/public/igem/BBa_K864102/annotation2197168/range2197168/1
type: Range
persistent identity: range2197168
display id: range2197168
version: 1
direction: direction/reverse
top level: BBa_K864102
start: 19
end: 741
orientation: inline

BBa_K733014
type: ComponentDefinition
persistent identity: BBa K733014
display id: BBa_K733014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg+RBS+ydjM+Bmp2
creator: Chris Yu Lai Cheong; LI Yiming
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K733006
component: BBa_K733013
sequence annotation: BBa_K733006
sequence annotation: BBa_K733013
sequence: https://synbiohub.org/public/igem/BBa_K733014_sequence/1

BBa_K733006
type: Component
persistent identity: component2183804
display id: component2183804
version: 1
top level: BBa_K733014
access: public
definition: BBa_K733006

BBa_K733013
type: Component
persistent identity: component2183803
display id: component2183803
version: 1
top level: BBa_K733014
access: public
definition: BBa_K733013

BBa_K733006
type: SequenceAnnotation
persistent identity: annotation2183804
display id: annotation2183804
version: 1
top level: BBa_K733014
location: https://synbiohub.org/public/igem/BBa_K733014/annotation2183804/range2183804/1
component: BBa_K733006

https://synbiohub.org/public/igem/BBa_K733014/annotation2183804/range2183804/1
type: Range
persistent identity: range2183804
display id: range2183804
version: 1
direction: direction/reverse
top level: BBa_K733014
start: 118
end: 578
orientation: inline

BBa_K733013
type: SequenceAnnotation
persistent identity: annotation2183803
display id: annotation2183803
version: 1
top level: BBa_K733014
location: https://synbiohub.org/public/igem/BBa_K733014/annotation2183803/range2183803/1
component: BBa_K733013

https://synbiohub.org/public/igem/BBa_K733014/annotation2183803/range2183803/1
type: Range
persistent identity: range2183803
display id: range2183803
version: 1
direction: direction/reverse
top level: BBa_K733014
start: 1
end: 109
orientation: inline

Ruxyn
type: ComponentDefinition
persistent identity: BBa K805012
display id: BBa_K805012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ruxyn1
creator: Liu Ping
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1062_
m user id: 0
owner id: 11895
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: test
mutable notes: test
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K805012_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2200609
display id: annotation2200609
version: 1
top level: Ruxyn
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K805012/annotation2200609/range2200609/1

https://synbiohub.org/public/igem/BBa_K805012/annotation2200609/range2200609/1
type: Range
persistent identity: range2200609
display id: range2200609
version: 1
direction: direction/forward
top level: Ruxyn
start: 4
end: 996
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2200513
display id: annotation2200513
version: 1
top level: Ruxyn
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K805012/annotation2200513/range2200513/1

https://synbiohub.org/public/igem/BBa_K805012/annotation2200513/range2200513/1
type: Range
persistent identity: range2200513
display id: range2200513
version: 1
direction: direction/forward
top level: Ruxyn
start: 1
end: 3
orientation: inline

BBa_K584003
type: ComponentDefinition
persistent identity: BBa K584003
display id: BBa_K584003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI and LuxR regulated - hybrid promoter + GFP generator
creator: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
created: 2011-08-15T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _755_
m user id: 0
owner id: 9879
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part was designed to test the functionality of the promotor region.
mutable notes: /
mutable provenance: /
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0065
component: BBa_E0240
sequence annotation: BBa_R0065
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K584003_sequence/1

BBa_R0065
type: Component
persistent identity: component2125160
display id: component2125160
version: 1
top level: BBa_K584003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_E0240
type: Component
persistent identity: component2125178
display id: component2125178
version: 1
top level: BBa_K584003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation2125160
display id: annotation2125160
version: 1
top level: BBa_K584003
location: https://synbiohub.org/public/igem/BBa_K584003/annotation2125160/range2125160/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_K584003/annotation2125160/range2125160/1
type: Range
persistent identity: range2125160
display id: range2125160
version: 1
direction: direction/forward
top level: BBa_K584003
start: 1
end: 97
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2125178
display id: annotation2125178
version: 1
top level: BBa_K584003
location: https://synbiohub.org/public/igem/BBa_K584003/annotation2125178/range2125178/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K584003/annotation2125178/range2125178/1
type: Range
persistent identity: range2125178
display id: range2125178
version: 1
direction: direction/reverse
top level: BBa_K584003
start: 106
end: 981
orientation: inline

BBa_K886000
type: ComponentDefinition
persistent identity: BBa K886000
display id: BBa_K886000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fixed lox71
creator: edgar andres ochoa cruz
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1149_
m user id: 0
owner id: 11298
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The particularity of lox71 is that it has an altered sequence at the end of it's right arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.
mutable notes: Based on the report made by the igem2010 UT-Tokyo team and some papers, the lox71 (BBa_I718017) from the registry was wrong, it had a cg instead a gc in its center sequence that is between the arms. Apparently, this part was corrected by the iGEM11_WITS_CSIR_SA team (BBa_K537020), but the sequence from this group had the same error. The corrected part from iGEM11_Tokyo_Tech (BBa_K649205) was right but no DNA was available in the registry. So we decided to synthesized it and test it, using the proper sequence described by http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/, and we submitted the correct DNA to the Registry.
mutable provenance: primers
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K886000_sequence/1

BBa_K561001
type: ComponentDefinition
persistent identity: BBa K561001
display id: BBa_K561001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vgb promoter, microaerobic
creator: HUA Yijie, MA Keyue, XIA Yu
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _729_
m user id: 0
owner id: 8637
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: vgb promoter is induced under microaerobic conditions.
mutable notes: We construct this promoter, according to the reference: "Construction of highly efficient E. coli expression systems containing low oxygen induced promoter and partition region" by Liu Tao et al.
mutable provenance: vgb promoter comes from Vitreoscilla sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: vgb promoter
sequence: https://synbiohub.org/public/igem/BBa_K561001_sequence/1

vgb promoter
type: SequenceAnnotation
persistent identity: annotation2146939
display id: annotation2146939
version: 1
top level: BBa_K561001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K561001/annotation2146939/range2146939/1

https://synbiohub.org/public/igem/BBa_K561001/annotation2146939/range2146939/1
type: Range
persistent identity: range2146939
display id: range2146939
version: 1
direction: direction/forward
top level: BBa_K561001
start: 1
end: 135
orientation: inline

BBa_K731721
type: ComponentDefinition
persistent identity: BBa K731721
display id: BBa_K731721
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 terminator
creator: Giacomo Giacomelli, Anna Depetris
created: 2012-08-14T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a short terminator by T7phage that we tested with our new teminators' characterization system.
mutable notes: This is a standard sequence, very used in many constructs. As i don't have any design considerations i can say that generally to make screening gels of such short segments is preferable to use TBE1X instead of TAE1X.
mutable provenance: We amplified it directly from pET21b.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T7 terminator
sequence: https://synbiohub.org/public/igem/BBa_K731721_sequence/1

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2180632
display id: annotation2180632
version: 1
top level: BBa_K731721
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731721/annotation2180632/range2180632/1

https://synbiohub.org/public/igem/BBa_K731721/annotation2180632/range2180632/1
type: Range
persistent identity: range2180632
display id: range2180632
version: 1
direction: direction/forward
top level: BBa_K731721
start: 1
end: 48
orientation: inline

BBa_K575009
type: ComponentDefinition
persistent identity: BBa K575009
display id: BBa_K575009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP
creator: Kristin Palarz
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The inducable Promoter for the LasR/PAI1 complex attached to RBS (B0034) and GFP.
mutable notes: Need to ensure no leaky transciption of GFP.
mutable provenance: From Biobrick Parts registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_R0079
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0079
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K575009_sequence/1

BBa_B0034
type: Component
persistent identity: component2124829
display id: component2124829
version: 1
top level: BBa_K575009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0079
type: Component
persistent identity: component2124827
display id: component2124827
version: 1
top level: BBa_K575009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_E0040
type: Component
persistent identity: component2124831
display id: component2124831
version: 1
top level: BBa_K575009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2124829
display id: annotation2124829
version: 1
top level: BBa_K575009
location: https://synbiohub.org/public/igem/BBa_K575009/annotation2124829/range2124829/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K575009/annotation2124829/range2124829/1
type: Range
persistent identity: range2124829
display id: range2124829
version: 1
direction: direction/reverse
top level: BBa_K575009
start: 166
end: 177
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2124827
display id: annotation2124827
version: 1
top level: BBa_K575009
location: https://synbiohub.org/public/igem/BBa_K575009/annotation2124827/range2124827/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K575009/annotation2124827/range2124827/1
type: Range
persistent identity: range2124827
display id: range2124827
version: 1
direction: direction/reverse
top level: BBa_K575009
start: 1
end: 157
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2124831
display id: annotation2124831
version: 1
top level: BBa_K575009
location: https://synbiohub.org/public/igem/BBa_K575009/annotation2124831/range2124831/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K575009/annotation2124831/range2124831/1
type: Range
persistent identity: range2124831
display id: range2124831
version: 1
direction: direction/reverse
top level: BBa_K575009
start: 184
end: 903
orientation: inline

BBa_K325909
type: ComponentDefinition
persistent identity: BBa K325909
display id: BBa_K325909
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux Operon (under pBAD) Vibrio Fischeri
creator: Theo Sanderson and Will Handley
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: G28
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: putative rbs
sequence annotation: luxE
sequence annotation: luxB
sequence annotation: PC
sequence annotation: luxA
sequence annotation: luxG
sequence annotation: AraC
sequence annotation: luxC
sequence annotation: luxD
sequence annotation: pBAD
sequence annotation: putative rbs region
sequence: https://synbiohub.org/public/igem/BBa_K325909_sequence/1

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112117
display id: annotation2112117
version: 1
top level: BBa_K325909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112117/range2112117/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112117/range2112117/1
type: Range
persistent identity: range2112117
display id: range2112117
version: 1
direction: direction/forward
top level: BBa_K325909
start: 3616
end: 3621
orientation: inline

luxE
type: SequenceAnnotation
persistent identity: annotation2112058
display id: annotation2112058
version: 1
top level: BBa_K325909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112058/range2112058/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112058/range2112058/1
type: Range
persistent identity: range2112058
display id: range2112058
version: 1
direction: direction/forward
top level: BBa_K325909
start: 5826
end: 6923
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112122
display id: annotation2112122
version: 1
top level: BBa_K325909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112122/range2112122/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112122/range2112122/1
type: Range
persistent identity: range2112122
display id: range2112122
version: 1
direction: direction/forward
top level: BBa_K325909
start: 6915
end: 6919
orientation: inline

luxB
type: SequenceAnnotation
persistent identity: annotation2112057
display id: annotation2112057
version: 1
top level: BBa_K325909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112057/range2112057/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112057/range2112057/1
type: Range
persistent identity: range2112057
display id: range2112057
version: 1
direction: direction/forward
top level: BBa_K325909
start: 4732
end: 5712
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2112036
display id: annotation2112036
version: 1
top level: BBa_K325909
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112036/range2112036/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112036/range2112036/1
type: Range
persistent identity: range2112036
display id: range2112036
version: 1
direction: direction/forward
top level: BBa_K325909
start: 1043
end: 1114
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112119
display id: annotation2112119
version: 1
top level: BBa_K325909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112119/range2112119/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112119/range2112119/1
type: Range
persistent identity: range2112119
display id: range2112119
version: 1
direction: direction/forward
top level: BBa_K325909
start: 5775
end: 5781
orientation: inline

luxA
type: SequenceAnnotation
persistent identity: annotation2112056
display id: annotation2112056
version: 1
top level: BBa_K325909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112056/range2112056/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112056/range2112056/1
type: Range
persistent identity: range2112056
display id: range2112056
version: 1
direction: direction/forward
top level: BBa_K325909
start: 3629
end: 4693
orientation: inline

luxG
type: SequenceAnnotation
persistent identity: annotation2112059
display id: annotation2112059
version: 1
top level: BBa_K325909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112059/range2112059/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112059/range2112059/1
type: Range
persistent identity: range2112059
display id: range2112059
version: 1
direction: direction/forward
top level: BBa_K325909
start: 6927
end: 7637
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2111897
display id: annotation2111897
version: 1
top level: BBa_K325909
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2111897/range2111897/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2111897/range2111897/1
type: Range
persistent identity: range2111897
display id: range2111897
version: 1
direction: direction/forward
top level: BBa_K325909
start: 1
end: 1079
orientation: inline

luxC
type: SequenceAnnotation
persistent identity: annotation2112039
display id: annotation2112039
version: 1
top level: BBa_K325909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112039/range2112039/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112039/range2112039/1
type: Range
persistent identity: range2112039
display id: range2112039
version: 1
direction: direction/forward
top level: BBa_K325909
start: 1231
end: 2660
orientation: inline

luxD
type: SequenceAnnotation
persistent identity: annotation2112076
display id: annotation2112076
version: 1
top level: BBa_K325909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112076/range2112076/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112076/range2112076/1
type: Range
persistent identity: range2112076
display id: range2112076
version: 1
direction: direction/forward
top level: BBa_K325909
start: 2675
end: 3578
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112062
display id: annotation2112062
version: 1
top level: BBa_K325909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112062/range2112062/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112062/range2112062/1
type: Range
persistent identity: range2112062
display id: range2112062
version: 1
direction: direction/forward
top level: BBa_K325909
start: 4719
end: 4724
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112061
display id: annotation2112061
version: 1
top level: BBa_K325909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112061/range2112061/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112061/range2112061/1
type: Range
persistent identity: range2112061
display id: range2112061
version: 1
direction: direction/forward
top level: BBa_K325909
start: 2664
end: 2668
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2112038
display id: annotation2112038
version: 1
top level: BBa_K325909
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112038/range2112038/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112038/range2112038/1
type: Range
persistent identity: range2112038
display id: range2112038
version: 1
direction: direction/forward
top level: BBa_K325909
start: 1080
end: 1210
orientation: inline

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2112060
display id: annotation2112060
version: 1
top level: BBa_K325909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112060/range2112060/1

https://synbiohub.org/public/igem/BBa_K325909/annotation2112060/range2112060/1
type: Range
persistent identity: range2112060
display id: range2112060
version: 1
direction: direction/forward
top level: BBa_K325909
start: 1211
end: 1230
orientation: inline

BBa_K575010
type: ComponentDefinition
persistent identity: BBa K575010
display id: BBa_K575010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP
creator: Kristin Palarz
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR/PAI1 inducable promoter with RBS (B0030) and RFP.
mutable notes: Need to ensure no leaky transcription.
mutable provenance: From BioBrick Parts Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_R0079
component: BBa_E1010
sequence annotation: BBa_B0030
sequence annotation: BBa_R0079
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K575010_sequence/1

BBa_B0030
type: Component
persistent identity: component2247836
display id: component2247836
version: 1
top level: BBa_K575010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0079
type: Component
persistent identity: component2247834
display id: component2247834
version: 1
top level: BBa_K575010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_E1010
type: Component
persistent identity: component2247840
display id: component2247840
version: 1
top level: BBa_K575010
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2247836
display id: annotation2247836
version: 1
top level: BBa_K575010
location: https://synbiohub.org/public/igem/BBa_K575010/annotation2247836/range2247836/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K575010/annotation2247836/range2247836/1
type: Range
persistent identity: range2247836
display id: range2247836
version: 1
direction: direction/forward
top level: BBa_K575010
start: 166
end: 180
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2247834
display id: annotation2247834
version: 1
top level: BBa_K575010
location: https://synbiohub.org/public/igem/BBa_K575010/annotation2247834/range2247834/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K575010/annotation2247834/range2247834/1
type: Range
persistent identity: range2247834
display id: range2247834
version: 1
direction: direction/reverse
top level: BBa_K575010
start: 1
end: 157
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247840
display id: annotation2247840
version: 1
top level: BBa_K575010
location: https://synbiohub.org/public/igem/BBa_K575010/annotation2247840/range2247840/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K575010/annotation2247840/range2247840/1
type: Range
persistent identity: range2247840
display id: range2247840
version: 1
direction: direction/reverse
top level: BBa_K575010
start: 187
end: 892
orientation: inline

BBa_K598003
type: ComponentDefinition
persistent identity: BBa K598003
display id: BBa_K598003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS
creator: Yangyang ZHAO, Yan GONG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 3541
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It's a basic part including only a ribozyme sequence numbered 2.5
mutable notes: Only use it by sites mutation
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: TPP Ribozyme 2.5
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K598003_sequence/1

TPP Ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2152899
display id: annotation2152899
version: 1
top level: BBa_K598003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598003/annotation2152899/range2152899/1

https://synbiohub.org/public/igem/BBa_K598003/annotation2152899/range2152899/1
type: Range
persistent identity: range2152899
display id: range2152899
version: 1
direction: direction/forward
top level: BBa_K598003
start: 1
end: 150
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2152707
display id: annotation2152707
version: 1
top level: BBa_K598003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598003/annotation2152707/range2152707/1

https://synbiohub.org/public/igem/BBa_K598003/annotation2152707/range2152707/1
type: Range
persistent identity: range2152707
display id: range2152707
version: 1
direction: direction/forward
top level: BBa_K598003
start: 138
end: 145
orientation: inline

BBa_K577893
type: ComponentDefinition
persistent identity: BBa K577893
display id: BBa_K577893
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressed GFP
creator: Kyle Jones
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _748_
m user id: 0
owner id: 8430
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A composite part containing the TetR gene, C0040, turned on by a constitutive promoter, I14033. The TetR represses the pTet promoter in front of the GFP gene. By addition of tetracycline or aTc, this repression is inhibited and the cells should fluoresce green.
mutable notes: The whole part is in the backbone of I14033. Both P0440, the TetR composite part, and E0240, the GFP composite part, were used and inserted into their respective promoters. The pTet+GFP part was then combined as a suffix insertion to the I14033+TetR backbone.
mutable provenance: Spring 2011 iGEM Kit Plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_C0040
component: BBa_B0034
component: BBa_E0040
component: BBa_I14033
component: BBa_B0032
component: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_C0040
sequence annotation: BBa_R0040
sequence annotation: BBa_I14033
sequence: https://synbiohub.org/public/igem/BBa_K577893_sequence/1

BBa_B0012
type: Component
persistent identity: component2289593
display id: component2289593
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2289591
display id: component2289591
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0040
type: Component
persistent identity: component2289574
display id: component2289574
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0010
type: Component
persistent identity: component2289575
display id: component2289575
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2289570
display id: component2289570
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2289590
display id: component2289590
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_I14033
type: Component
persistent identity: component2289568
display id: component2289568
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_I14033/1

BBa_B0032
type: Component
persistent identity: component2289587
display id: component2289587
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component2289577
display id: component2289577
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component2289581
display id: component2289581
version: 1
top level: BBa_K577893
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2289577
display id: annotation2289577
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289577/range2289577/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K577893/annotation2289577/range2289577/1
type: Range
persistent identity: range2289577
display id: range2289577
version: 1
direction: direction/forward
top level: BBa_K577893
start: 846
end: 886
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2289593
display id: annotation2289593
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289593/range2289593/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K577893/annotation2289593/range2289593/1
type: Range
persistent identity: range2289593
display id: range2289593
version: 1
direction: direction/forward
top level: BBa_K577893
start: 1792
end: 1832
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2289587
display id: annotation2289587
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289587/range2289587/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K577893/annotation2289587/range2289587/1
type: Range
persistent identity: range2289587
display id: range2289587
version: 1
direction: direction/forward
top level: BBa_K577893
start: 957
end: 969
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2289591
display id: annotation2289591
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289591/range2289591/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K577893/annotation2289591/range2289591/1
type: Range
persistent identity: range2289591
display id: range2289591
version: 1
direction: direction/forward
top level: BBa_K577893
start: 1704
end: 1783
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2289575
display id: annotation2289575
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289575/range2289575/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K577893/annotation2289575/range2289575/1
type: Range
persistent identity: range2289575
display id: range2289575
version: 1
direction: direction/forward
top level: BBa_K577893
start: 758
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2289570
display id: annotation2289570
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289570/range2289570/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K577893/annotation2289570/range2289570/1
type: Range
persistent identity: range2289570
display id: range2289570
version: 1
direction: direction/reverse
top level: BBa_K577893
start: 47
end: 58
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2289590
display id: annotation2289590
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289590/range2289590/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K577893/annotation2289590/range2289590/1
type: Range
persistent identity: range2289590
display id: range2289590
version: 1
direction: direction/reverse
top level: BBa_K577893
start: 976
end: 1695
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2289574
display id: annotation2289574
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289574/range2289574/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K577893/annotation2289574/range2289574/1
type: Range
persistent identity: range2289574
display id: range2289574
version: 1
direction: direction/reverse
top level: BBa_K577893
start: 65
end: 749
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2289581
display id: annotation2289581
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289581/range2289581/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K577893/annotation2289581/range2289581/1
type: Range
persistent identity: range2289581
display id: range2289581
version: 1
direction: direction/forward
top level: BBa_K577893
start: 895
end: 948
orientation: inline

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2289568
display id: annotation2289568
version: 1
top level: BBa_K577893
location: https://synbiohub.org/public/igem/BBa_K577893/annotation2289568/range2289568/1
component: BBa_I14033

https://synbiohub.org/public/igem/BBa_K577893/annotation2289568/range2289568/1
type: Range
persistent identity: range2289568
display id: range2289568
version: 1
direction: direction/reverse
top level: BBa_K577893
start: 1
end: 38
orientation: inline

Sod Cu/Zn
type: ComponentDefinition
persistent identity: BBa K847004
display id: BBa_K847004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Deinococcus radiodurans'' Superoxide dismutase, SodCu/Zn
creator: Rashmi Sharma, Vishesh Jain, Kendrick Wang
created: 2012-08-08T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1107_
m user id: 0
owner id: 11092
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tba
mutable notes: tba
mutable provenance: tba
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K847004_sequence/1

BBa_K598010
type: ComponentDefinition
persistent identity: BBa K598010
display id: BBa_K598010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+Theophylline Responsive Riboswitch 1G1 with Engineered RBS+E0040+B0015
creator: Siyang HAO, Zhenrun ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8816
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is a theophylline responsive riboswitch which contains a designed RBS.
mutable notes: Check the RBS sequence before using.
mutable provenance: By PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: eGFP
sequence annotation: Engineered RBS
sequence annotation: pBAD
sequence annotation: Theo 1G1
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K598010_sequence/1

eGFP
type: SequenceAnnotation
persistent identity: annotation2150242
display id: annotation2150242
version: 1
top level: BBa_K598010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598010/annotation2150242/range2150242/1

https://synbiohub.org/public/igem/BBa_K598010/annotation2150242/range2150242/1
type: Range
persistent identity: range2150242
display id: range2150242
version: 1
direction: direction/forward
top level: BBa_K598010
start: 225
end: 944
orientation: inline

Engineered RBS
type: SequenceAnnotation
persistent identity: annotation2150245
display id: annotation2150245
version: 1
top level: BBa_K598010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598010/annotation2150245/range2150245/1

https://synbiohub.org/public/igem/BBa_K598010/annotation2150245/range2150245/1
type: Range
persistent identity: range2150245
display id: range2150245
version: 1
direction: direction/forward
top level: BBa_K598010
start: 213
end: 219
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2150240
display id: annotation2150240
version: 1
top level: BBa_K598010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598010/annotation2150240/range2150240/1

https://synbiohub.org/public/igem/BBa_K598010/annotation2150240/range2150240/1
type: Range
persistent identity: range2150240
display id: range2150240
version: 1
direction: direction/forward
top level: BBa_K598010
start: 1
end: 130
orientation: inline

Theo 1G1
type: SequenceAnnotation
persistent identity: annotation2150241
display id: annotation2150241
version: 1
top level: BBa_K598010
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598010/annotation2150241/range2150241/1

https://synbiohub.org/public/igem/BBa_K598010/annotation2150241/range2150241/1
type: Range
persistent identity: range2150241
display id: range2150241
version: 1
direction: direction/forward
top level: BBa_K598010
start: 139
end: 224
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2150244
display id: annotation2150244
version: 1
top level: BBa_K598010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598010/annotation2150244/range2150244/1

https://synbiohub.org/public/igem/BBa_K598010/annotation2150244/range2150244/1
type: Range
persistent identity: range2150244
display id: range2150244
version: 1
direction: direction/forward
top level: BBa_K598010
start: 945
end: 1081
orientation: inline

M-B
type: ComponentDefinition
persistent identity: BBa K774001
display id: BBa_K774001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mammalian-Bacterial Promoter: E9-ns2 CArG promoter sequence + BHN + BBaK216005
creator: NRP-UEA-Norwich
created: 2012-06-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1026_
m user id: 0
owner id: 11706
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: A hybrid of the PyeaR promoter (BBaK216005) and the E9-ns2 CArG promoter (Ref1). Two versions will be synthesised, with the promoters altering their position in relation to the 5???-end of the sequence.
mutable notes: To provide additional restriction enzyme sites that may become useful during later cloning steps, BamHI, HindIII and NdeI have been added between the 2 promoters. It is envisaged that any open reading frame (e.g. RFP or GFP) will be cloned ???downstream??? (i.e. at the 3???-end) of these promoter sequences.
mutable provenance: PyeaR promoter (BBaK216005) and the E9-ns2 CArG promoter (Ref1) Ref1: Scott, S.D., Joiner, M.C. & Marples, B., 2002. Optimizing radiation-responsive gene promoters for radiogenetic cancer therapy. Gene therapy, 9(20), p.1396-402. Available at: http://www.ncbi.nlm.nih.gov/pubmed/12365005 [Accessed June 24, 2012].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBaK216005
sequence annotation: BHN
sequence annotation: E9-ns2 CArG promoter
sequence: https://synbiohub.org/public/igem/BBa_K774001_sequence/1

BBaK216005
type: SequenceAnnotation
persistent identity: annotation2177194
display id: annotation2177194
version: 1
top level: M-B
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K774001/annotation2177194/range2177194/1

https://synbiohub.org/public/igem/BBa_K774001/annotation2177194/range2177194/1
type: Range
persistent identity: range2177194
display id: range2177194
version: 1
direction: direction/forward
top level: M-B
start: 110
end: 208
orientation: inline

BHN
type: SequenceAnnotation
persistent identity: annotation2177193
display id: annotation2177193
version: 1
top level: M-B
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K774001/annotation2177193/range2177193/1

https://synbiohub.org/public/igem/BBa_K774001/annotation2177193/range2177193/1
type: Range
persistent identity: range2177193
display id: range2177193
version: 1
direction: direction/forward
top level: M-B
start: 91
end: 109
orientation: inline

E9-ns2 CArG promoter
type: SequenceAnnotation
persistent identity: annotation2177192
display id: annotation2177192
version: 1
top level: M-B
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K774001/annotation2177192/range2177192/1

https://synbiohub.org/public/igem/BBa_K774001/annotation2177192/range2177192/1
type: Range
persistent identity: range2177192
display id: range2177192
version: 1
direction: direction/forward
top level: M-B
start: 1
end: 90
orientation: inline

BBa_K530008
type: ComponentDefinition
persistent identity: BBa K530008
display id: BBa_K530008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TDH3 Yeast Promoter
creator: Daniel Wolozny
created: 2011-08-14T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _696_
m user id: 0
owner id: 8447
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
mutable notes: Promoter was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the promoter.
mutable provenance: http://www.yeastgenome.org/cgi-bin/locus.fpl
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TDH3 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K530008_sequence/1

TDH3 Promoter
type: SequenceAnnotation
persistent identity: annotation2125804
display id: annotation2125804
version: 1
top level: BBa_K530008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K530008/annotation2125804/range2125804/1

https://synbiohub.org/public/igem/BBa_K530008/annotation2125804/range2125804/1
type: Range
persistent identity: range2125804
display id: range2125804
version: 1
direction: direction/forward
top level: BBa_K530008
start: 1
end: 500
orientation: inline

BBa_K660004
type: ComponentDefinition
persistent identity: BBa K660004
display id: BBa_K660004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: iLOV
creator: Glasgow Uni 2011
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:13:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _837_
m user id: 0
owner id: 9421
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: iLOV has the same function and uses as LOV2. As a reporter, it is advantageous over GFP derived fluorescent proteins due to its small size (useful if you are tagging proteins), ability to recover quickly from photobleaching and use in anoxic conditions.
mutable notes: illegal restriction sites. The part was synthesized to remove them.
mutable provenance: iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: iLOV Domain
sequence: https://synbiohub.org/public/igem/BBa_K660004_sequence/1

iLOV Domain
type: SequenceAnnotation
persistent identity: annotation2135615
display id: annotation2135615
version: 1
top level: BBa_K660004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K660004/annotation2135615/range2135615/1

https://synbiohub.org/public/igem/BBa_K660004/annotation2135615/range2135615/1
type: Range
persistent identity: range2135615
display id: range2135615
version: 1
direction: direction/forward
top level: BBa_K660004
start: 1
end: 336
orientation: inline

BBa_K574004
type: ComponentDefinition
persistent identity: BBa K574004
display id: BBa_K574004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL regulated by TetR
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: p(tetR) + lasI
mutable notes: Empty.
mutable provenance: Existing Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K081016
component: BBa_R0040
sequence annotation: BBa_K081016
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K574004_sequence/1

BBa_K081016
type: Component
persistent identity: component2250774
display id: component2250774
version: 1
top level: BBa_K574004
access: public
definition: https://synbiohub.org/public/igem/BBa_K081016/1

BBa_R0040
type: Component
persistent identity: component2250757
display id: component2250757
version: 1
top level: BBa_K574004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K081016
type: SequenceAnnotation
persistent identity: annotation2250774
display id: annotation2250774
version: 1
top level: BBa_K574004
location: https://synbiohub.org/public/igem/BBa_K574004/annotation2250774/range2250774/1
component: BBa_K081016

https://synbiohub.org/public/igem/BBa_K574004/annotation2250774/range2250774/1
type: Range
persistent identity: range2250774
display id: range2250774
version: 1
direction: direction/reverse
top level: BBa_K574004
start: 63
end: 797
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2250757
display id: annotation2250757
version: 1
top level: BBa_K574004
location: https://synbiohub.org/public/igem/BBa_K574004/annotation2250757/range2250757/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K574004/annotation2250757/range2250757/1
type: Range
persistent identity: range2250757
display id: range2250757
version: 1
direction: direction/forward
top level: BBa_K574004
start: 1
end: 54
orientation: inline

BBa_K325219
type: ComponentDefinition
persistent identity: BBa K325219
display id: BBa_K325219
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red Firefly Luciferase and LRE (under pBAD) L. Cruciata (E. coli optimised)
creator: Bill Collins and Emily Knott
created: 2010-09-14T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata). It also generates the luciferin regenerating enzyme. When luciferin and D-cysteine are added this BioBrick should give sustained light output. With simply luciferin it will give a burst of light output.
mutable notes: Biobrick assembly
mutable provenance: Biobrick assembly from its two subparts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K325210
sequence annotation: BBa_K325210
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K325219_sequence/1

BBa_I0500
type: Component
persistent identity: component2103897
display id: component2103897
version: 1
top level: BBa_K325219
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K325210
type: Component
persistent identity: component2103906
display id: component2103906
version: 1
top level: BBa_K325219
access: public
definition: BBa_K325210

BBa_K325210
type: SequenceAnnotation
persistent identity: annotation2103906
display id: annotation2103906
version: 1
top level: BBa_K325219
location: https://synbiohub.org/public/igem/BBa_K325219/annotation2103906/range2103906/1
component: BBa_K325210

https://synbiohub.org/public/igem/BBa_K325219/annotation2103906/range2103906/1
type: Range
persistent identity: range2103906
display id: range2103906
version: 1
direction: direction/reverse
top level: BBa_K325219
start: 1219
end: 3841
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2103897
display id: annotation2103897
version: 1
top level: BBa_K325219
location: https://synbiohub.org/public/igem/BBa_K325219/annotation2103897/range2103897/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K325219/annotation2103897/range2103897/1
type: Range
persistent identity: range2103897
display id: range2103897
version: 1
direction: direction/reverse
top level: BBa_K325219
start: 1
end: 1210
orientation: inline

BBa_K929301
type: ComponentDefinition
persistent identity: BBa K929301
display id: BBa_K929301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Potsdam Standard Backbone
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14525
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The Potsdam Assembly Standard is a modified PLICing (Phosphorothioate-based ligase-independent gene cloning) method ([http://www.ncbi.nlm.nih.gov/pubmed/20646988 Blanusa ''et al.'' (2010)]). For this standard we designed a new cloning backbone with an RFP expression cassette as insert and two new restriction enzyme recognition sites in the suffix and prefix in pSB1C3, the Potsdam Standard Backbone. In the prefix, we added the Apa I recognition site and in the suffix the Sph I recognition site. Both enzymes causing a 3??? overhang with 4 nucleotides. For the cloning process we cut the vector with Apa I and Sph I. In this assembly standard that???s the only step where we have to use restriction enzymes. The gene of interest was amplified with primers that contain 4 phosphothioate nucleotides and the recognition sites for Apa I and Sph I at the 5??? end. After incubation in an iodine/ethanol solution, the thiophosphates were cut out resulting in a 3??? overhang which is suitable to the overhangs that was created by cutting the new assembly vector. After that the digested vector and the PLICed insert were mixed and transformed into E. coli. By using the RFP expression cassette we created a ligation control system. Due to the fact that red fluorescent colonies have a failed vector ligation we can tell which colonies are correctly ligated. The colonies that do not show red fluorescence are the positive clones.
mutable notes: To create the Potsdam Standard Backbone, we amplified the RFP expression cassette (BBa_J04450) to add the restriction site for Apa I and Sph I. Then, the amplified expression cassette was insert into pSB1C3 using Xba I and Pst I.
mutable provenance: BBa_J04450
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LacI
sequence annotation: RFP
sequence annotation: Apa I
sequence annotation: BBa B0034
sequence annotation: Sph I
sequence annotation: Cap
sequence: https://synbiohub.org/public/igem/BBa_K929301_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation2203448
display id: annotation2203448
version: 1
top level: BBa_K929301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K929301/annotation2203448/range2203448/1

https://synbiohub.org/public/igem/BBa_K929301/annotation2203448/range2203448/1
type: Range
persistent identity: range2203448
display id: range2203448
version: 1
direction: direction/forward
top level: BBa_K929301
start: 83
end: 118
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2203450
display id: annotation2203450
version: 1
top level: BBa_K929301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K929301/annotation2203450/range2203450/1

https://synbiohub.org/public/igem/BBa_K929301/annotation2203450/range2203450/1
type: Range
persistent identity: range2203450
display id: range2203450
version: 1
direction: direction/forward
top level: BBa_K929301
start: 145
end: 822
orientation: inline

Apa I
type: SequenceAnnotation
persistent identity: annotation2203446
display id: annotation2203446
version: 1
top level: BBa_K929301
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929301/annotation2203446/range2203446/1

https://synbiohub.org/public/igem/BBa_K929301/annotation2203446/range2203446/1
type: Range
persistent identity: range2203446
display id: range2203446
version: 1
direction: direction/forward
top level: BBa_K929301
start: 1
end: 6
orientation: inline

BBa B0034
type: SequenceAnnotation
persistent identity: annotation2203449
display id: annotation2203449
version: 1
top level: BBa_K929301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K929301/annotation2203449/range2203449/1

https://synbiohub.org/public/igem/BBa_K929301/annotation2203449/range2203449/1
type: Range
persistent identity: range2203449
display id: range2203449
version: 1
direction: direction/forward
top level: BBa_K929301
start: 127
end: 138
orientation: inline

Sph I
type: SequenceAnnotation
persistent identity: annotation2203451
display id: annotation2203451
version: 1
top level: BBa_K929301
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929301/annotation2203451/range2203451/1

https://synbiohub.org/public/igem/BBa_K929301/annotation2203451/range2203451/1
type: Range
persistent identity: range2203451
display id: range2203451
version: 1
direction: direction/forward
top level: BBa_K929301
start: 823
end: 828
orientation: inline

Cap
type: SequenceAnnotation
persistent identity: annotation2203447
display id: annotation2203447
version: 1
top level: BBa_K929301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K929301/annotation2203447/range2203447/1

https://synbiohub.org/public/igem/BBa_K929301/annotation2203447/range2203447/1
type: Range
persistent identity: range2203447
display id: range2203447
version: 1
direction: direction/forward
top level: BBa_K929301
start: 7
end: 44
orientation: inline

Y taRNA
type: ComponentDefinition
persistent identity: BBa K606034
display id: BBa_K606034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tyrosine amber supressor tRNA
creator: Cyrille Pauthenier
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tyrosine amber suppressor tRNA for B. Subtilis.
mutable notes: This sequence is the pure tRNA without the side sequences
mutable provenance: Synthetize de novo by matching primers and cloned from the tRNA cluster of B. Subtilis genome of strain 168
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K606034_sequence/1

BBa_K608003
type: ComponentDefinition
persistent identity: BBa K608003
display id: BBa_K608003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong Promoter , medium RBS
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: strong Promotor , medium RBS
mutable notes: composite
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23104
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K608003_sequence/1

BBa_J23104
type: Component
persistent identity: component2128649
display id: component2128649
version: 1
top level: BBa_K608003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23104/1

BBa_B0032
type: Component
persistent identity: component2128651
display id: component2128651
version: 1
top level: BBa_K608003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128651
display id: annotation2128651
version: 1
top level: BBa_K608003
location: https://synbiohub.org/public/igem/BBa_K608003/annotation2128651/range2128651/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K608003/annotation2128651/range2128651/1
type: Range
persistent identity: range2128651
display id: range2128651
version: 1
direction: direction/forward
top level: BBa_K608003
start: 44
end: 56
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128649
display id: annotation2128649
version: 1
top level: BBa_K608003
location: https://synbiohub.org/public/igem/BBa_K608003/annotation2128649/range2128649/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608003/annotation2128649/range2128649/1
type: Range
persistent identity: range2128649
display id: range2128649
version: 1
direction: direction/reverse
top level: BBa_K608003
start: 1
end: 35
orientation: inline

BBa_K617003
type: ComponentDefinition
persistent identity: BBa K617003
display id: BBa_K617003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R6K origin of replication
creator: Will Jones
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _789_
m user id: 0
owner id: 4301
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: conditional origin of replication
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: dna
sequence: https://synbiohub.org/public/igem/BBa_K617003_sequence/1

dna
type: SequenceAnnotation
persistent identity: annotation2148712
display id: annotation2148712
version: 1
top level: BBa_K617003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K617003/annotation2148712/range2148712/1

https://synbiohub.org/public/igem/BBa_K617003/annotation2148712/range2148712/1
type: Range
persistent identity: range2148712
display id: range2148712
version: 1
direction: direction/forward
top level: BBa_K617003
start: 1
end: 392
orientation: inline

BBa_K538101
type: ComponentDefinition
persistent identity: BBa K538101
display id: BBa_K538101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Cpn60
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K538001
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K538001
sequence: https://synbiohub.org/public/igem/BBa_K538101_sequence/1

BBa_K538001
type: Component
persistent identity: component2133885
display id: component2133885
version: 1
top level: BBa_K538101
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_B0034
type: Component
persistent identity: component2133881
display id: component2133881
version: 1
top level: BBa_K538101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133881
display id: annotation2133881
version: 1
top level: BBa_K538101
location: https://synbiohub.org/public/igem/BBa_K538101/annotation2133881/range2133881/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538101/annotation2133881/range2133881/1
type: Range
persistent identity: range2133881
display id: range2133881
version: 1
direction: direction/reverse
top level: BBa_K538101
start: 1
end: 12
orientation: inline

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2133885
display id: annotation2133885
version: 1
top level: BBa_K538101
location: https://synbiohub.org/public/igem/BBa_K538101/annotation2133885/range2133885/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538101/annotation2133885/range2133885/1
type: Range
persistent identity: range2133885
display id: range2133885
version: 1
direction: direction/reverse
top level: BBa_K538101
start: 19
end: 1668
orientation: inline

BBa_K553020
type: ComponentDefinition
persistent identity: BBa K553020
display id: BBa_K553020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mammaliam Secreted Beta Lactamase
creator: Luca Braga, Niels Ntamati
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _721_
m user id: 0
owner id: 8729
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The coding sequence is composed from the N-Terminus to the C-Terminus by the secretion signal of human IgG in fusion with the TEM-1 beta lactamase.
mutable notes: This basic part has been designed in order to have a prefix and a suffix according to the standard Biobrick structure.
mutable provenance: Synthetic origin, it has been designed according to its functions in our final system.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K553020_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2127674
display id: annotation2127674
version: 1
top level: BBa_K553020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K553020/annotation2127674/range2127674/1

https://synbiohub.org/public/igem/BBa_K553020/annotation2127674/range2127674/1
type: Range
persistent identity: range2127674
display id: range2127674
version: 1
direction: direction/forward
top level: BBa_K553020
start: 631
end: 633
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2127673
display id: annotation2127673
version: 1
top level: BBa_K553020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K553020/annotation2127673/range2127673/1

https://synbiohub.org/public/igem/BBa_K553020/annotation2127673/range2127673/1
type: Range
persistent identity: range2127673
display id: range2127673
version: 1
direction: direction/forward
top level: BBa_K553020
start: 4
end: 7
orientation: inline

BBa_K598011
type: ComponentDefinition
persistent identity: BBa K598011
display id: BBa_K598011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015
creator: Yangyang ZHAO, Zhenrun ZHANG, Yan GONG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 3541
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a basic part that contains pBAD promoter, a ribozyme sequence numbered 2.5, a GFP part named E0040 and a terminator part named B0015.
mutable notes: Use it by stantard assembly or by sites mutation
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: eGFP
sequence annotation: RBS
sequence annotation: TPP Ribozyme 2.5
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: pBAD
sequence: https://synbiohub.org/public/igem/BBa_K598011_sequence/1

eGFP
type: SequenceAnnotation
persistent identity: annotation2152712
display id: annotation2152712
version: 1
top level: BBa_K598011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598011/annotation2152712/range2152712/1

https://synbiohub.org/public/igem/BBa_K598011/annotation2152712/range2152712/1
type: Range
persistent identity: range2152712
display id: range2152712
version: 1
direction: direction/forward
top level: BBa_K598011
start: 288
end: 1007
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2144565
display id: annotation2144565
version: 1
top level: BBa_K598011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598011/annotation2144565/range2144565/1

https://synbiohub.org/public/igem/BBa_K598011/annotation2144565/range2144565/1
type: Range
persistent identity: range2144565
display id: range2144565
version: 1
direction: direction/forward
top level: BBa_K598011
start: 275
end: 282
orientation: inline

TPP Ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2152717
display id: annotation2152717
version: 1
top level: BBa_K598011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598011/annotation2152717/range2152717/1

https://synbiohub.org/public/igem/BBa_K598011/annotation2152717/range2152717/1
type: Range
persistent identity: range2152717
display id: range2152717
version: 1
direction: direction/forward
top level: BBa_K598011
start: 137
end: 287
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2154348
display id: annotation2154348
version: 1
top level: BBa_K598011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598011/annotation2154348/range2154348/1

https://synbiohub.org/public/igem/BBa_K598011/annotation2154348/range2154348/1
type: Range
persistent identity: range2154348
display id: range2154348
version: 1
direction: direction/forward
top level: BBa_K598011
start: 288
end: 1007
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2154351
display id: annotation2154351
version: 1
top level: BBa_K598011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598011/annotation2154351/range2154351/1

https://synbiohub.org/public/igem/BBa_K598011/annotation2154351/range2154351/1
type: Range
persistent identity: range2154351
display id: range2154351
version: 1
direction: direction/forward
top level: BBa_K598011
start: 1016
end: 1144
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2152713
display id: annotation2152713
version: 1
top level: BBa_K598011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598011/annotation2152713/range2152713/1

https://synbiohub.org/public/igem/BBa_K598011/annotation2152713/range2152713/1
type: Range
persistent identity: range2152713
display id: range2152713
version: 1
direction: direction/forward
top level: BBa_K598011
start: 1
end: 130
orientation: inline

YF1
type: ComponentDefinition
persistent identity: BBa K592004
display id: BBa_K592004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YF1 blue-light sensor protein
creator: Lei Sun
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.
mutable notes: The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.
mutable provenance: Ytva comes from the genome of Bacillus subtilis. FixL derives from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YF1
sequence: https://synbiohub.org/public/igem/BBa_K592004_sequence/1

YF1
type: SequenceAnnotation
persistent identity: annotation2130111
display id: annotation2130111
version: 1
top level: YF1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1

https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
type: Range
persistent identity: range2130111
display id: range2130111
version: 1
direction: direction/forward
top level: YF1
start: 1
end: 1131
orientation: inline

BBa_K530014
type: ComponentDefinition
persistent identity: BBa K530014
display id: BBa_K530014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KRE9 Yeast UTR
creator: Daniel Wolozny
created: 2011-09-02T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
group u list: _696_
m user id: 0
owner id: 8447
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: KRE9 yeast UTR is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
mutable notes: UTR was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the UTR.
mutable provenance: http://www.yeastgenome.org/cgi-bin/locus.fpl
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: KRE9 UTR
sequence: https://synbiohub.org/public/igem/BBa_K530014_sequence/1

KRE9 UTR
type: SequenceAnnotation
persistent identity: annotation2125809
display id: annotation2125809
version: 1
top level: BBa_K530014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K530014/annotation2125809/range2125809/1

https://synbiohub.org/public/igem/BBa_K530014/annotation2125809/range2125809/1
type: Range
persistent identity: range2125809
display id: range2125809
version: 1
direction: direction/forward
top level: BBa_K530014
start: 1
end: 221
orientation: inline

BBa_K576003
type: ComponentDefinition
persistent identity: BBa K576003
display id: BBa_K576003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Left Part of Self-Excising Ribozyme
creator: George Zarubin
created: 2011-06-19T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
group u list: _747_
m user id: 0
owner id: 7330
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Modified 3' "half" of intron found in ORF #142 in Twort phage of Staphylococcus aureus in RFC53 MetaPart format. When complemented with 5' "half" ( BBa_K576004 ) in complience with BBFRFC53 assembly standard, the RNA-level excision should leave GGU linker between the whatever comes before K576003 and after K576004. K576003 and K576004 and everything in between them would be removed and possibly circularized.
mutable notes: The P1 helix of the intron has been modified to accommodate the MetaPrefix. The part is in RFC53 L-MetaPart format, having GGT and ATG overgangs.
mutable provenance: [http://www.rna.whu.edu.cn/perl/gissd/get_seq_exon.cgi?intron_id=304 Group I intron Twort.v.ORF142,2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: IN1
sequence annotation: MetaPrefix
sequence annotation: MetaSuffix
sequence: https://synbiohub.org/public/igem/BBa_K576003_sequence/1

IN1
type: SequenceAnnotation
persistent identity: annotation2121633
display id: annotation2121633
version: 1
top level: BBa_K576003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K576003/annotation2121633/range2121633/1

https://synbiohub.org/public/igem/BBa_K576003/annotation2121633/range2121633/1
type: Range
persistent identity: range2121633
display id: range2121633
version: 1
direction: direction/forward
top level: BBa_K576003
start: 11
end: 119
orientation: inline

MetaPrefix
type: SequenceAnnotation
persistent identity: annotation2121632
display id: annotation2121632
version: 1
top level: BBa_K576003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K576003/annotation2121632/range2121632/1

https://synbiohub.org/public/igem/BBa_K576003/annotation2121632/range2121632/1
type: Range
persistent identity: range2121632
display id: range2121632
version: 1
direction: direction/forward
top level: BBa_K576003
start: 1
end: 10
orientation: inline

MetaSuffix
type: SequenceAnnotation
persistent identity: annotation2121634
display id: annotation2121634
version: 1
top level: BBa_K576003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K576003/annotation2121634/range2121634/1

https://synbiohub.org/public/igem/BBa_K576003/annotation2121634/range2121634/1
type: Range
persistent identity: range2121634
display id: range2121634
version: 1
direction: direction/forward
top level: BBa_K576003
start: 120
end: 129
orientation: inline

BBa_K554000
type: ComponentDefinition
persistent identity: BBa K554000
display id: BBa_K554000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SoxS promoter
creator: UNICAMP_EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: SoxS promoter
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SoxS
sequence: https://synbiohub.org/public/igem/BBa_K554000_sequence/1

SoxS
type: SequenceAnnotation
persistent identity: annotation2136873
display id: annotation2136873
version: 1
top level: BBa_K554000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K554000/annotation2136873/range2136873/1

https://synbiohub.org/public/igem/BBa_K554000/annotation2136873/range2136873/1
type: Range
persistent identity: range2136873
display id: range2136873
version: 1
direction: direction/forward
top level: BBa_K554000
start: 1
end: 62
orientation: inline

BBa_S05060
type: ComponentDefinition
persistent identity: BBa S05060
display id: BBa_S05060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K873000:B0015
creator: yue hu
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:14:49Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 11547
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_K873000
sequence annotation: BBa_B0015
sequence annotation: BBa_K873000
sequence: https://synbiohub.org/public/igem/BBa_S05060_sequence/1

BBa_B0015
type: Component
persistent identity: component2185413
display id: component2185413
version: 1
top level: BBa_S05060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K873000
type: Component
persistent identity: component2185406
display id: component2185406
version: 1
top level: BBa_S05060
access: public
definition: https://synbiohub.org/public/igem/BBa_K873000/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2185413
display id: annotation2185413
version: 1
top level: BBa_S05060
location: https://synbiohub.org/public/igem/BBa_S05060/annotation2185413/range2185413/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S05060/annotation2185413/range2185413/1
type: Range
persistent identity: range2185413
display id: range2185413
version: 1
direction: direction/reverse
top level: BBa_S05060
start: 303
end: 431
orientation: inline

BBa_K873000
type: SequenceAnnotation
persistent identity: annotation2185406
display id: annotation2185406
version: 1
top level: BBa_S05060
location: https://synbiohub.org/public/igem/BBa_S05060/annotation2185406/range2185406/1
component: BBa_K873000

https://synbiohub.org/public/igem/BBa_S05060/annotation2185406/range2185406/1
type: Range
persistent identity: range2185406
display id: range2185406
version: 1
direction: direction/reverse
top level: BBa_S05060
start: 1
end: 294
orientation: inline

BBa_K624003
type: ComponentDefinition
persistent identity: BBa K624003
display id: BBa_K624003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rep gene
creator: SHANG-JUI TSAI, CHUN-YU LAI
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _796_
m user id: 0
owner id: 6664
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: only the plasmid contain rep gene can be used in magnetic bacteria AMB-1
mutable notes: yes
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rep (pMGT)
sequence annotation: start
sequence annotation: rbs from pMGT
sequence: https://synbiohub.org/public/igem/BBa_K624003_sequence/1

rep (pMGT)
type: SequenceAnnotation
persistent identity: annotation2148115
display id: annotation2148115
version: 1
top level: BBa_K624003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K624003/annotation2148115/range2148115/1

https://synbiohub.org/public/igem/BBa_K624003/annotation2148115/range2148115/1
type: Range
persistent identity: range2148115
display id: range2148115
version: 1
direction: direction/forward
top level: BBa_K624003
start: 28
end: 857
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148127
display id: annotation2148127
version: 1
top level: BBa_K624003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K624003/annotation2148127/range2148127/1

https://synbiohub.org/public/igem/BBa_K624003/annotation2148127/range2148127/1
type: Range
persistent identity: range2148127
display id: range2148127
version: 1
direction: direction/forward
top level: BBa_K624003
start: 25
end: 27
orientation: inline

rbs from pMGT
type: SequenceAnnotation
persistent identity: annotation2148116
display id: annotation2148116
version: 1
top level: BBa_K624003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K624003/annotation2148116/range2148116/1

https://synbiohub.org/public/igem/BBa_K624003/annotation2148116/range2148116/1
type: Range
persistent identity: range2148116
display id: range2148116
version: 1
direction: direction/forward
top level: BBa_K624003
start: 1
end: 24
orientation: inline

BBa_K934012
type: ComponentDefinition
persistent identity: BBa K934012
display id: BBa_K934012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plas-LuxI
creator: Toshiki Hashimoto
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1199_
m user id: 0
owner id: 14028
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: We constructed this part by combining K649000 and K081008. This part starts generating LuxI enzyme in the presence of LasR-3OC12HSL complex.
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K649000
component: BBa_K081008
sequence annotation: BBa_K649000
sequence annotation: BBa_K081008
sequence: https://synbiohub.org/public/igem/BBa_K934012_sequence/1

BBa_K649000
type: Component
persistent identity: component2248169
display id: component2248169
version: 1
top level: BBa_K934012
access: public
definition: https://synbiohub.org/public/igem/BBa_K649000/1

BBa_K081008
type: Component
persistent identity: component2248177
display id: component2248177
version: 1
top level: BBa_K934012
access: public
definition: https://synbiohub.org/public/igem/BBa_K081008/1

BBa_K649000
type: SequenceAnnotation
persistent identity: annotation2248169
display id: annotation2248169
version: 1
top level: BBa_K934012
location: https://synbiohub.org/public/igem/BBa_K934012/annotation2248169/range2248169/1
component: BBa_K649000

https://synbiohub.org/public/igem/BBa_K934012/annotation2248169/range2248169/1
type: Range
persistent identity: range2248169
display id: range2248169
version: 1
direction: direction/reverse
top level: BBa_K934012
start: 1
end: 83
orientation: inline

BBa_K081008
type: SequenceAnnotation
persistent identity: annotation2248177
display id: annotation2248177
version: 1
top level: BBa_K934012
location: https://synbiohub.org/public/igem/BBa_K934012/annotation2248177/range2248177/1
component: BBa_K081008

https://synbiohub.org/public/igem/BBa_K934012/annotation2248177/range2248177/1
type: Range
persistent identity: range2248177
display id: range2248177
version: 1
direction: direction/reverse
top level: BBa_K934012
start: 92
end: 755
orientation: inline

BBa_K813001
type: ComponentDefinition
persistent identity: BBa K813001
display id: BBa_K813001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: URE2 - Yeast Genomic IRES
creator: Morgan Baltz, Katie Lutes, Akshitha Yarrabothula, Michael Wierzbicki
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1070_
m user id: 0
owner id: 11253
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: IRES elements initiate 5'- independent translation.
mutable notes: NONE
mutable provenance: saccharomyces cerevisiae genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K813001_sequence/1

BBa_K733009
type: ComponentDefinition
persistent identity: BBa K733009
display id: BBa_K733009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptms+BBa_E0240: Ptms+RBS+GFP+Double
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0240
component: BBa_K733001
sequence annotation: BBa_E0240
sequence annotation: BBa_K733001
sequence: https://synbiohub.org/public/igem/BBa_K733009_sequence/1

BBa_E0240
type: Component
persistent identity: component2183743
display id: component2183743
version: 1
top level: BBa_K733009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K733001
type: Component
persistent identity: component2183732
display id: component2183732
version: 1
top level: BBa_K733009
access: public
definition: BBa_K733001

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2183743
display id: annotation2183743
version: 1
top level: BBa_K733009
location: https://synbiohub.org/public/igem/BBa_K733009/annotation2183743/range2183743/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K733009/annotation2183743/range2183743/1
type: Range
persistent identity: range2183743
display id: range2183743
version: 1
direction: direction/reverse
top level: BBa_K733009
start: 74
end: 949
orientation: inline

BBa_K733001
type: SequenceAnnotation
persistent identity: annotation2183732
display id: annotation2183732
version: 1
top level: BBa_K733009
location: https://synbiohub.org/public/igem/BBa_K733009/annotation2183732/range2183732/1
component: BBa_K733001

https://synbiohub.org/public/igem/BBa_K733009/annotation2183732/range2183732/1
type: Range
persistent identity: range2183732
display id: range2183732
version: 1
direction: direction/reverse
top level: BBa_K733009
start: 1
end: 65
orientation: inline

BBa_K822001
type: ComponentDefinition
persistent identity: BBa K822001
display id: BBa_K822001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ldhA promoter + RBS from Corynebacterium glutamicum
creator: Jarle Pahr
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1080_
m user id: 0
owner id: 11627
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Promoter region associated with the ldhA operon of C. glutamicum.
mutable notes: N/A
mutable provenance: C. glutamicum genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K822001_sequence/1

FabH2
type: ComponentDefinition
persistent identity: BBa K590034
display id: BBa_K590034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FabH2
creator: Matthew Harger
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Makes Branched Chain Alkanes
mutable notes: None
mutable provenance: TBD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K590034_sequence/1

YAP1
type: ComponentDefinition
persistent identity: BBa K813000
display id: BBa_K813000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YAP1 - Yeast Genomic IRES
creator: Morgan Baltz, Katie Lutes, Akshitha Yarrabothula, Michael Wierzbicki
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1070_
m user id: 0
owner id: 11253
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: IRES elements initiate 5'- independent translation.
mutable notes: NONE
mutable provenance: saccharomyces cerevisiae genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K813000_sequence/1

BBa_K542010
type: ComponentDefinition
persistent identity: BBa K542010
display id: BBa_K542010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Enhanced Lumazine Synthase (ELS)
creator: Anthony Vuong
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The part was synthesized by Bio Basic Inc. into the pET28a plasmid vector with an N-terminal His-tag. The Enhanced Lumazine Synthase (ELS) without the His-tag was moved into the pSB1C3 plasmid vector by the Lethbridge 2011 team. The size of the cavity of the Lumazine Synthase microcompartment was enlarged via directed evolution (W??rsd??rfer ''et al.,'' 2011). The compartment formed by this polypeptide has a larger cavity than the Lumazine Synthase microcompartment submitted by the Lethbridge 2009 team ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002]). A larger interior allows for the option of localizing larger and/or more proteins into the cavity of the compartment. Reference: W??rsd??rfer, B., Woycechowsky, K.J., and Hilvert, D., (2011). Directed Evolution of a Protein Container. ''Science.'' 331: 589-592.
mutable notes: The compartment formed by this polypeptide has a larger cavity than the Lumazine Synthase microcompartment submitted by the Lethbridge 2009 team ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002]).
mutable provenance: The Enhanced Lumazine Synthase (ELS) sequence was the result of directed evolution. The part was synthesized by Bio Basic Inc. and moved into pSB1C3 by the Lethbridge 2011 team. W??rsd??rfer, B., Woycechowsky, K.J., and Hilvert, D., (2011). Directed Evolution of a Protein Container. ''Science.'' 331: 589-592.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K542010_sequence/1

BBa_K539626
type: ComponentDefinition
persistent identity: BBa K539626
display id: BBa_K539626
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a RBS (B0034)+ilvD
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K539712
component: BBa_B0034
sequence annotation: BBa_K539712
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K539626_sequence/1

BBa_K539712
type: Component
persistent identity: component2143909
display id: component2143909
version: 1
top level: BBa_K539626
access: public
definition: https://synbiohub.org/public/igem/BBa_K539712/1

BBa_B0034
type: Component
persistent identity: component2143907
display id: component2143907
version: 1
top level: BBa_K539626
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K539712
type: SequenceAnnotation
persistent identity: annotation2143909
display id: annotation2143909
version: 1
top level: BBa_K539626
location: https://synbiohub.org/public/igem/BBa_K539626/annotation2143909/range2143909/1
component: BBa_K539712

https://synbiohub.org/public/igem/BBa_K539626/annotation2143909/range2143909/1
type: Range
persistent identity: range2143909
display id: range2143909
version: 1
direction: direction/reverse
top level: BBa_K539626
start: 19
end: 1869
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2143907
display id: annotation2143907
version: 1
top level: BBa_K539626
location: https://synbiohub.org/public/igem/BBa_K539626/annotation2143907/range2143907/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K539626/annotation2143907/range2143907/1
type: Range
persistent identity: range2143907
display id: range2143907
version: 1
direction: direction/reverse
top level: BBa_K539626
start: 1
end: 12
orientation: inline

BBa_K598005
type: ComponentDefinition
persistent identity: BBa K598005
display id: BBa_K598005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline Responsive Riboswitch P1G1 with Native RBS
creator: Siyang HAO
created: 2011-08-14T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8597
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a test to add a new part.1
mutable notes: This is a test to add a new part.3
mutable provenance: This is a test to add a new part.2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Native RBS
sequence annotation: Theophylline responsive riboswitch Theo P1G1
sequence: https://synbiohub.org/public/igem/BBa_K598005_sequence/1

Native RBS
type: SequenceAnnotation
persistent identity: annotation2150192
display id: annotation2150192
version: 1
top level: BBa_K598005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598005/annotation2150192/range2150192/1

https://synbiohub.org/public/igem/BBa_K598005/annotation2150192/range2150192/1
type: Range
persistent identity: range2150192
display id: range2150192
version: 1
direction: direction/forward
top level: BBa_K598005
start: 75
end: 78
orientation: inline

Theophylline responsive riboswitch Theo P1G1
type: SequenceAnnotation
persistent identity: annotation2152450
display id: annotation2152450
version: 1
top level: BBa_K598005
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598005/annotation2152450/range2152450/1

https://synbiohub.org/public/igem/BBa_K598005/annotation2152450/range2152450/1
type: Range
persistent identity: range2152450
display id: range2152450
version: 1
direction: direction/forward
top level: BBa_K598005
start: 1
end: 87
orientation: inline

BBa_K524001
type: ComponentDefinition
persistent identity: BBa K524001
display id: BBa_K524001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac + RBS + split sfGFP 1-10
creator: HO Yuan Heng Trevor
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _689_
m user id: 0
owner id: 8758
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: (to be entered)
mutable notes: A stop codon was added to the end of the CDS to terminate translation of sfGFP1-10
mutable provenance: Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop codon
sequence annotation: B0034
sequence annotation: start codon
sequence annotation: R0010
sequence annotation: sfGFP1-10
sequence: https://synbiohub.org/public/igem/BBa_K524001_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2150868
display id: annotation2150868
version: 1
top level: BBa_K524001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K524001/annotation2150868/range2150868/1

https://synbiohub.org/public/igem/BBa_K524001/annotation2150868/range2150868/1
type: Range
persistent identity: range2150868
display id: range2150868
version: 1
direction: direction/forward
top level: BBa_K524001
start: 868
end: 873
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2146555
display id: annotation2146555
version: 1
top level: BBa_K524001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K524001/annotation2146555/range2146555/1

https://synbiohub.org/public/igem/BBa_K524001/annotation2146555/range2146555/1
type: Range
persistent identity: range2146555
display id: range2146555
version: 1
direction: direction/forward
top level: BBa_K524001
start: 208
end: 219
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2150867
display id: annotation2150867
version: 1
top level: BBa_K524001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K524001/annotation2150867/range2150867/1

https://synbiohub.org/public/igem/BBa_K524001/annotation2150867/range2150867/1
type: Range
persistent identity: range2150867
display id: range2150867
version: 1
direction: direction/forward
top level: BBa_K524001
start: 226
end: 228
orientation: inline

R0010
type: SequenceAnnotation
persistent identity: annotation2146554
display id: annotation2146554
version: 1
top level: BBa_K524001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K524001/annotation2146554/range2146554/1

https://synbiohub.org/public/igem/BBa_K524001/annotation2146554/range2146554/1
type: Range
persistent identity: range2146554
display id: range2146554
version: 1
direction: direction/forward
top level: BBa_K524001
start: 1
end: 200
orientation: inline

sfGFP1-10
type: SequenceAnnotation
persistent identity: annotation2146556
display id: annotation2146556
version: 1
top level: BBa_K524001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K524001/annotation2146556/range2146556/1

https://synbiohub.org/public/igem/BBa_K524001/annotation2146556/range2146556/1
type: Range
persistent identity: range2146556
display id: range2146556
version: 1
direction: direction/forward
top level: BBa_K524001
start: 226
end: 873
orientation: inline

NPDC-1C3-H
type: ComponentDefinition
persistent identity: BBa K590028
display id: BBa_K590028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NPDC-PSB1C3-High constitutive
creator: Matthew Harger, Lei Zheng
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Nostoc punctiforme PCC73102 Aldehyde Decarbonylase in PSB1C3 high consititutive vector
mutable notes: None
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K590033
component: BBa_K314100
sequence annotation: BBa_K314100
sequence annotation: BBa_K590033
sequence: https://synbiohub.org/public/igem/BBa_K590028_sequence/1

BBa_K590033
type: Component
persistent identity: component2129135
display id: component2129135
version: 1
top level: NPDC-1C3-H
access: public
definition: https://synbiohub.org/public/igem/BBa_K590033/1

BBa_K314100
type: Component
persistent identity: component2129134
display id: component2129134
version: 1
top level: NPDC-1C3-H
access: public
definition: BBa_K314100

BBa_K314100
type: SequenceAnnotation
persistent identity: annotation2129134
display id: annotation2129134
version: 1
top level: NPDC-1C3-H
location: https://synbiohub.org/public/igem/BBa_K590028/annotation2129134/range2129134/1
component: BBa_K314100

https://synbiohub.org/public/igem/BBa_K590028/annotation2129134/range2129134/1
type: Range
persistent identity: range2129134
display id: range2129134
version: 1
direction: direction/reverse
top level: NPDC-1C3-H
start: 1
end: 518
orientation: inline

BBa_K590033
type: SequenceAnnotation
persistent identity: annotation2129135
display id: annotation2129135
version: 1
top level: NPDC-1C3-H
location: https://synbiohub.org/public/igem/BBa_K590028/annotation2129135/range2129135/1
component: BBa_K590033

https://synbiohub.org/public/igem/BBa_K590028/annotation2129135/range2129135/1
type: Range
persistent identity: range2129135
display id: range2129135
version: 1
direction: direction/reverse
top level: NPDC-1C3-H
start: 525
end: 1250
orientation: inline

BBa_K592027
type: ComponentDefinition
persistent identity: BBa K592027
display id: BBa_K592027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T5-lac promoter-B0032-BFP
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter T5-lac ( BBa_K592008 ) for testing its promoter strength coupled with BFP ( BBa_K592100 ). This construct should be cloned into pSB3K3 plasmid to measure the BFP output and thereby determine the promoter strength. Theoretically, the promoter should be well-repressed in LacIq strain but inducible by high concentrations of IPTG.
mutable notes: This part was assembled into pSB3K3 for testing, and later cloned into pSB1C3 for submission.
mutable provenance: The information of BBa_K592008 and BBa_K592100 can be found in the registry. T5-lac promoter is a fusion promoter with parts from T5 phage.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0032
component: BBa_K592008
component: BBa_K592100
sequence annotation: BBa_K592100
sequence annotation: BBa_B0032
sequence annotation: BBa_K592008
sequence: https://synbiohub.org/public/igem/BBa_K592027_sequence/1

BBa_B0032
type: Component
persistent identity: component2136783
display id: component2136783
version: 1
top level: BBa_K592027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K592008
type: Component
persistent identity: component2136781
display id: component2136781
version: 1
top level: BBa_K592027
access: public
definition: PT5lac

BBa_K592100
type: Component
persistent identity: component2136786
display id: component2136786
version: 1
top level: BBa_K592027
access: public
definition: BFP

BBa_K592100
type: SequenceAnnotation
persistent identity: annotation2136786
display id: annotation2136786
version: 1
top level: BBa_K592027
location: https://synbiohub.org/public/igem/BBa_K592027/annotation2136786/range2136786/1
component: BBa_K592100

https://synbiohub.org/public/igem/BBa_K592027/annotation2136786/range2136786/1
type: Range
persistent identity: range2136786
display id: range2136786
version: 1
direction: direction/reverse
top level: BBa_K592027
start: 154
end: 858
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2136783
display id: annotation2136783
version: 1
top level: BBa_K592027
location: https://synbiohub.org/public/igem/BBa_K592027/annotation2136783/range2136783/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K592027/annotation2136783/range2136783/1
type: Range
persistent identity: range2136783
display id: range2136783
version: 1
direction: direction/forward
top level: BBa_K592027
start: 135
end: 147
orientation: inline

BBa_K592008
type: SequenceAnnotation
persistent identity: annotation2136781
display id: annotation2136781
version: 1
top level: BBa_K592027
location: https://synbiohub.org/public/igem/BBa_K592027/annotation2136781/range2136781/1
component: BBa_K592008

https://synbiohub.org/public/igem/BBa_K592027/annotation2136781/range2136781/1
type: Range
persistent identity: range2136781
display id: range2136781
version: 1
direction: direction/reverse
top level: BBa_K592027
start: 1
end: 126
orientation: inline

9A1
type: ComponentDefinition
persistent identity: BBa K590057
display id: BBa_K590057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RrD_Y21W-PSB1C3-High constitutive
creator: Lei Zheng, Ben Mo, Elaine Lai, Juhye An
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Plasmid that consists of Acyl-ACP Reductase rbs mutant Synechococcus elongatus PCC7942 Aldehyde Decarbonylase in PSB1C3 high consititutive vector for alkane production
mutable notes: TBD
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K590032
component: BBa_B0034
component: BBa_K590040
component: BBa_K314100
sequence annotation: BBa_K590040
sequence annotation: BBa_K314100
sequence annotation: BBa_K590032
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K590057_sequence/1

BBa_K590032
type: Component
persistent identity: component2129367
display id: component2129367
version: 1
top level: 9A1
access: public
definition: https://synbiohub.org/public/igem/BBa_K590032/1

BBa_B0034
type: Component
persistent identity: component2129369
display id: component2129369
version: 1
top level: 9A1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K590040
type: Component
persistent identity: component2129370
display id: component2129370
version: 1
top level: 9A1
access: public
definition: https://synbiohub.org/public/igem/BBa_K590040/1

BBa_K314100
type: Component
persistent identity: component2129366
display id: component2129366
version: 1
top level: 9A1
access: public
definition: BBa_K314100

BBa_K590040
type: SequenceAnnotation
persistent identity: annotation2129370
display id: annotation2129370
version: 1
top level: 9A1
location: https://synbiohub.org/public/igem/BBa_K590057/annotation2129370/range2129370/1
component: BBa_K590040

https://synbiohub.org/public/igem/BBa_K590057/annotation2129370/range2129370/1
type: Range
persistent identity: range2129370
display id: range2129370
version: 1
direction: direction/reverse
top level: 9A1
start: 1637
end: 2392
orientation: inline

BBa_K314100
type: SequenceAnnotation
persistent identity: annotation2129366
display id: annotation2129366
version: 1
top level: 9A1
location: https://synbiohub.org/public/igem/BBa_K590057/annotation2129366/range2129366/1
component: BBa_K314100

https://synbiohub.org/public/igem/BBa_K590057/annotation2129366/range2129366/1
type: Range
persistent identity: range2129366
display id: range2129366
version: 1
direction: direction/reverse
top level: 9A1
start: 1
end: 518
orientation: inline

BBa_K590032
type: SequenceAnnotation
persistent identity: annotation2129367
display id: annotation2129367
version: 1
top level: 9A1
location: https://synbiohub.org/public/igem/BBa_K590057/annotation2129367/range2129367/1
component: BBa_K590032

https://synbiohub.org/public/igem/BBa_K590057/annotation2129367/range2129367/1
type: Range
persistent identity: range2129367
display id: range2129367
version: 1
direction: direction/reverse
top level: 9A1
start: 525
end: 1610
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2129369
display id: annotation2129369
version: 1
top level: 9A1
location: https://synbiohub.org/public/igem/BBa_K590057/annotation2129369/range2129369/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K590057/annotation2129369/range2129369/1
type: Range
persistent identity: range2129369
display id: range2129369
version: 1
direction: direction/reverse
top level: 9A1
start: 1619
end: 1630
orientation: inline

Tat PROM
type: ComponentDefinition
persistent identity: BBa K895000
display id: BBa_K895000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eco_Ptat2
creator: Frank Sargent
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1160_
m user id: 0
owner id: 8083
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The constitutive promoter region (97 bp) from the Escherichia coli K-12 tatABCD (twin-arginine translocase) operon. This is a constitutive promoter that gives good levels of expression in E. coli, but is not inducible or repressible. The promoter region is cloned as an EcoRI / PstI fragment into pSB1C3 following Biobrick Standard [10]. This is a corrected part that replaces the BBa_K562000 biobrick.
mutable notes: The part has an engineered BamHI (5'-GGATCC-3') restriction site at the extreme 3' end. For best results, open reading frames should be cloned into this BamHI site with their ATG initiation codons immediately following it. This will place the ATG at exactly the correct distance from the native tatA RBS for optimal translational efficiency.
mutable provenance: Amplified by PCR from BBa_K562000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K895000_sequence/1

PchiP-lacZ
type: ComponentDefinition
persistent identity: BBa K564012
display id: BBa_K564012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter and RBS of E. coli chitoporin translationally fused with lacZ.
creator: Anna Lewinska
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _732_
m user id: 0
owner id: 8333
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.
mutable notes: There are no design considerations.
mutable provenance: The subparts were prepared: ChiP upstream part from Escherichia Coli W3110 genomic DNA by PCR and lacZ from part BBa_I732005 present in part registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0010
component: BBa_K564004
component: BBa_I732005
sequence annotation: BBa_K564004
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I732005
sequence: https://synbiohub.org/public/igem/BBa_K564012_sequence/1

BBa_B0012
type: Component
persistent identity: component2135124
display id: component2135124
version: 1
top level: PchiP-lacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2135122
display id: component2135122
version: 1
top level: PchiP-lacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K564004
type: Component
persistent identity: component2135116
display id: component2135116
version: 1
top level: PchiP-lacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_K564004/1

BBa_I732005
type: Component
persistent identity: component2135121
display id: component2135121
version: 1
top level: PchiP-lacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_I732005/1

BBa_K564004
type: SequenceAnnotation
persistent identity: annotation2135116
display id: annotation2135116
version: 1
top level: PchiP-lacZ
location: https://synbiohub.org/public/igem/BBa_K564012/annotation2135116/range2135116/1
component: BBa_K564004

https://synbiohub.org/public/igem/BBa_K564012/annotation2135116/range2135116/1
type: Range
persistent identity: range2135116
display id: range2135116
version: 1
direction: direction/reverse
top level: PchiP-lacZ
start: 1
end: 599
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2135124
display id: annotation2135124
version: 1
top level: PchiP-lacZ
location: https://synbiohub.org/public/igem/BBa_K564012/annotation2135124/range2135124/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K564012/annotation2135124/range2135124/1
type: Range
persistent identity: range2135124
display id: range2135124
version: 1
direction: direction/forward
top level: PchiP-lacZ
start: 3777
end: 3817
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2135122
display id: annotation2135122
version: 1
top level: PchiP-lacZ
location: https://synbiohub.org/public/igem/BBa_K564012/annotation2135122/range2135122/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K564012/annotation2135122/range2135122/1
type: Range
persistent identity: range2135122
display id: range2135122
version: 1
direction: direction/forward
top level: PchiP-lacZ
start: 3689
end: 3768
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2135121
display id: annotation2135121
version: 1
top level: PchiP-lacZ
location: https://synbiohub.org/public/igem/BBa_K564012/annotation2135121/range2135121/1
component: BBa_I732005

https://synbiohub.org/public/igem/BBa_K564012/annotation2135121/range2135121/1
type: Range
persistent identity: range2135121
display id: range2135121
version: 1
direction: direction/reverse
top level: PchiP-lacZ
start: 606
end: 3680
orientation: inline

BBa_K539643
type: ComponentDefinition
persistent identity: BBa K539643
display id: BBa_K539643
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a RBS (B0034)+ilvC with a RBS (B0034)+ilvD
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K539626
component: BBa_K539623
sequence annotation: BBa_K539626
sequence annotation: BBa_K539623
sequence: https://synbiohub.org/public/igem/BBa_K539643_sequence/1

BBa_K539626
type: Component
persistent identity: component2143972
display id: component2143972
version: 1
top level: BBa_K539643
access: public
definition: BBa_K539626

BBa_K539623
type: Component
persistent identity: component2143967
display id: component2143967
version: 1
top level: BBa_K539643
access: public
definition: BBa_K539623

BBa_K539626
type: SequenceAnnotation
persistent identity: annotation2143972
display id: annotation2143972
version: 1
top level: BBa_K539643
location: https://synbiohub.org/public/igem/BBa_K539643/annotation2143972/range2143972/1
component: BBa_K539626

https://synbiohub.org/public/igem/BBa_K539643/annotation2143972/range2143972/1
type: Range
persistent identity: range2143972
display id: range2143972
version: 1
direction: direction/reverse
top level: BBa_K539643
start: 1503
end: 3371
orientation: inline

BBa_K539623
type: SequenceAnnotation
persistent identity: annotation2143967
display id: annotation2143967
version: 1
top level: BBa_K539643
location: https://synbiohub.org/public/igem/BBa_K539643/annotation2143967/range2143967/1
component: BBa_K539623

https://synbiohub.org/public/igem/BBa_K539643/annotation2143967/range2143967/1
type: Range
persistent identity: range2143967
display id: range2143967
version: 1
direction: direction/reverse
top level: BBa_K539643
start: 1
end: 1494
orientation: inline

BBa_K823000
type: ComponentDefinition
persistent identity: BBa K823000
display id: BBa_K823000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PliaG
creator: Korinna Kraft
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter of the liaG gene of Bacillus subtilis, weak constitutive
mutable notes: no considerations
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: PliaG
sequence: https://synbiohub.org/public/igem/BBa_K823000_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182555
display id: annotation2182555
version: 1
top level: BBa_K823000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182555/range2182555/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182555/range2182555/1
type: Range
persistent identity: range2182555
display id: range2182555
version: 1
direction: direction/forward
top level: BBa_K823000
start: 105
end: 110
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182554
display id: annotation2182554
version: 1
top level: BBa_K823000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182554/range2182554/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182554/range2182554/1
type: Range
persistent identity: range2182554
display id: range2182554
version: 1
direction: direction/forward
top level: BBa_K823000
start: 81
end: 86
orientation: inline

PliaG
type: SequenceAnnotation
persistent identity: annotation2182553
display id: annotation2182553
version: 1
top level: BBa_K823000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182553/range2182553/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182553/range2182553/1
type: Range
persistent identity: range2182553
display id: range2182553
version: 1
direction: direction/forward
top level: BBa_K823000
start: 1
end: 121
orientation: inline

BBa_K539421
type: ComponentDefinition
persistent identity: BBa K539421
display id: BBa_K539421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)+RNA thermometer
creator: Shu-Han Chang
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_K115002
sequence annotation: BBa_R0010
sequence annotation: BBa_K115002
sequence: https://synbiohub.org/public/igem/BBa_K539421_sequence/1

BBa_R0010
type: Component
persistent identity: component2143728
display id: component2143728
version: 1
top level: BBa_K539421
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K115002
type: Component
persistent identity: component2143739
display id: component2143739
version: 1
top level: BBa_K539421
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2143728
display id: annotation2143728
version: 1
top level: BBa_K539421
location: https://synbiohub.org/public/igem/BBa_K539421/annotation2143728/range2143728/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K539421/annotation2143728/range2143728/1
type: Range
persistent identity: range2143728
display id: range2143728
version: 1
direction: direction/forward
top level: BBa_K539421
start: 1
end: 200
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2143739
display id: annotation2143739
version: 1
top level: BBa_K539421
location: https://synbiohub.org/public/igem/BBa_K539421/annotation2143739/range2143739/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K539421/annotation2143739/range2143739/1
type: Range
persistent identity: range2143739
display id: range2143739
version: 1
direction: direction/reverse
top level: BBa_K539421
start: 209
end: 260
orientation: inline

BBa_K649001
type: ComponentDefinition
persistent identity: BBa K649001
display id: BBa_K649001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP regulated by 3OC12-HSL and LasR
creator: Tomoyuki Ohno
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _826_
m user id: 0
owner id: 9681
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP reporter regulated by 3OC12-HSL and LasR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J54103
component: BBa_K649000
sequence annotation: BBa_J54103
sequence annotation: BBa_K649000
sequence: https://synbiohub.org/public/igem/BBa_K649001_sequence/1

BBa_J54103
type: Component
persistent identity: component2152562
display id: component2152562
version: 1
top level: BBa_K649001
access: public
definition: https://synbiohub.org/public/igem/BBa_J54103/1

BBa_K649000
type: Component
persistent identity: component2152547
display id: component2152547
version: 1
top level: BBa_K649001
access: public
definition: https://synbiohub.org/public/igem/BBa_K649000/1

BBa_J54103
type: SequenceAnnotation
persistent identity: annotation2152562
display id: annotation2152562
version: 1
top level: BBa_K649001
location: https://synbiohub.org/public/igem/BBa_K649001/annotation2152562/range2152562/1
component: BBa_J54103

https://synbiohub.org/public/igem/BBa_K649001/annotation2152562/range2152562/1
type: Range
persistent identity: range2152562
display id: range2152562
version: 1
direction: direction/reverse
top level: BBa_K649001
start: 92
end: 1018
orientation: inline

BBa_K649000
type: SequenceAnnotation
persistent identity: annotation2152547
display id: annotation2152547
version: 1
top level: BBa_K649001
location: https://synbiohub.org/public/igem/BBa_K649001/annotation2152547/range2152547/1
component: BBa_K649000

https://synbiohub.org/public/igem/BBa_K649001/annotation2152547/range2152547/1
type: Range
persistent identity: range2152547
display id: range2152547
version: 1
direction: direction/reverse
top level: BBa_K649001
start: 1
end: 83
orientation: inline

BBa_K516011
type: ComponentDefinition
persistent identity: BBa K516011
display id: BBa_K516011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI protein generator TERM- RBS+(B0031)
creator: Nicolo' Politi, Tommaso Goggia, Paolo Magni
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: luxI protein generator TERM- RBS+(B0031)
mutable notes: --
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_K516011_sequence/1

BBa_C0061
type: Component
persistent identity: component2244637
display id: component2244637
version: 1
top level: BBa_K516011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0031
type: Component
persistent identity: component2244635
display id: component2244635
version: 1
top level: BBa_K516011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2244635
display id: annotation2244635
version: 1
top level: BBa_K516011
location: https://synbiohub.org/public/igem/BBa_K516011/annotation2244635/range2244635/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K516011/annotation2244635/range2244635/1
type: Range
persistent identity: range2244635
display id: range2244635
version: 1
direction: direction/reverse
top level: BBa_K516011
start: 1
end: 14
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2244637
display id: annotation2244637
version: 1
top level: BBa_K516011
location: https://synbiohub.org/public/igem/BBa_K516011/annotation2244637/range2244637/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K516011/annotation2244637/range2244637/1
type: Range
persistent identity: range2244637
display id: range2244637
version: 1
direction: direction/forward
top level: BBa_K516011
start: 21
end: 638
orientation: inline

BBa_K519010
type: ComponentDefinition
persistent identity: BBa K519010
display id: BBa_K519010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmtA
creator: Kotone Miyake
created: 2011-09-29T11:00:00Z
modified: 2016-01-13T10:24:13Z
discontinued: false
dominant: true
experience: experience/None
group u list: _684_
m user id: 4206
owner id: 9603
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: SmtA is a metallothionein that can bind to heavy metal ions such as Cd(II). This SmtA is cloned from Synechococcus sp. PCC7942 and it has been reported that the cyanobacterial strains that express SmtA can resist medium containing cadmim.
mutable notes: This part was cloned from Synechococcus sp. PCC7942, using primers to add EcoRI, XbaI and SpeI at the ends.
mutable provenance: Synechococcus sp. PCC7942
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K519010_sequence/1

BBa_K864204
type: ComponentDefinition
persistent identity: BBa K864204
display id: BBa_K864204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpF
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OmpF
mutable notes: Illegal PstI restriction site removed by silent mutations T>A at position 66 and A>T at position 69 using primers listed below (annealing sequences are underlined) ompF_rsr_F CTGAAATCTATAACAAAGATGGCAA ompF_rsr_R CTGCGTTTGCAGTACCTGCT
mutable provenance: Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined) ompF_BB_F TTCGAATTCGCGGCCGCTTCTAGATGATGAAGCGCAATATTC ompF_BB_R CGGCCGCTACTAGTATTATTAGAACTGGTAAACGATACC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A>T
sequence annotation: ompF
sequence annotation: T>A
sequence: https://synbiohub.org/public/igem/BBa_K864204_sequence/1

A>T
type: SequenceAnnotation
persistent identity: annotation2197201
display id: annotation2197201
version: 1
top level: BBa_K864204
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K864204/annotation2197201/range2197201/1

https://synbiohub.org/public/igem/BBa_K864204/annotation2197201/range2197201/1
type: Range
persistent identity: range2197201
display id: range2197201
version: 1
direction: direction/forward
top level: BBa_K864204
start: 69
end: 69
orientation: inline

ompF
type: SequenceAnnotation
persistent identity: annotation2197199
display id: annotation2197199
version: 1
top level: BBa_K864204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864204/annotation2197199/range2197199/1

https://synbiohub.org/public/igem/BBa_K864204/annotation2197199/range2197199/1
type: Range
persistent identity: range2197199
display id: range2197199
version: 1
direction: direction/forward
top level: BBa_K864204
start: 1
end: 1092
orientation: inline

T>A
type: SequenceAnnotation
persistent identity: annotation2197200
display id: annotation2197200
version: 1
top level: BBa_K864204
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K864204/annotation2197200/range2197200/1

https://synbiohub.org/public/igem/BBa_K864204/annotation2197200/range2197200/1
type: Range
persistent identity: range2197200
display id: range2197200
version: 1
direction: direction/forward
top level: BBa_K864204
start: 66
end: 66
orientation: inline

BBa_K748002
type: ComponentDefinition
persistent identity: BBa K748002
display id: BBa_K748002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Truncated lysostaphin coding sequence. Lysostaphin has has a specific lytic action against S.aureus.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lysostaphin is a zinc metalloenzyme that has a specific lytic action against S.aureus. Lysostaphin has activities of three enzymes namely, endo-β-N-acetyl glucosamidase, N-acteyl-muramyl-L-alanine amidase, glycylglycine endopeptidase. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the poly-glycine bridges which form cross links between glycopeptide chains in cell wall peptidoglycan of S.aureus cells. The wild-type lysostaphin gene encodes a preproenzyme, and the conversion of prolysostaphin to mature lysostaphin occurs extracellularly and involves the removal of the hydrophilic tandem repeat portion of the proenzyme. In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
mutable notes: In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K748002_sequence/1

BBa_K592016
type: ComponentDefinition
persistent identity: BBa K592016
display id: BBa_K592016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: YF1 works in conjunction with its response regulator, FixJ. This part consists of RBS-coupled YF1 and FixJ without promoter.
mutable notes: This constructs Strong RBS to ensure good expression.
mutable provenance: The components that made up of YF1 fusion protein comes from Bradyrhizobium japonicum and Bacillus subtilis. FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592005
component: BBa_K592004
component: BBa_B0034
sequence annotation: BBa_K592005
sequence annotation: BBa_B0034
sequence annotation: BBa_K592004
sequence: https://synbiohub.org/public/igem/BBa_K592016_sequence/1

BBa_K592005
type: Component
persistent identity: component2136339
display id: component2136339
version: 1
top level: BBa_K592016
access: public
definition: https://synbiohub.org/public/igem/BBa_K592005/1

BBa_K592004
type: Component
persistent identity: component2136335
display id: component2136335
version: 1
top level: BBa_K592016
access: public
definition: YF1

BBa_B0034
type: Component
persistent identity: component2136333
display id: component2136333
version: 1
top level: BBa_K592016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2136337
display id: component2136337
version: 1
top level: BBa_K592016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592005
type: SequenceAnnotation
persistent identity: annotation2136339
display id: annotation2136339
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
component: BBa_K592005

https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
type: Range
persistent identity: range2136339
display id: range2136339
version: 1
direction: direction/reverse
top level: BBa_K592016
start: 1176
end: 1796
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136333
display id: annotation2136333
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
type: Range
persistent identity: range2136333
display id: range2136333
version: 1
direction: direction/reverse
top level: BBa_K592016
start: 1
end: 12
orientation: inline

BBa_K592004
type: SequenceAnnotation
persistent identity: annotation2136335
display id: annotation2136335
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
component: BBa_K592004

https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
type: Range
persistent identity: range2136335
display id: range2136335
version: 1
direction: direction/reverse
top level: BBa_K592016
start: 19
end: 1149
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136337
display id: annotation2136337
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
type: Range
persistent identity: range2136337
display id: range2136337
version: 1
direction: direction/reverse
top level: BBa_K592016
start: 1158
end: 1169
orientation: inline

cheZ
type: ComponentDefinition
persistent identity: BBa K629003
display id: BBa_K629003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ, chemotaxis regulator, protein phosphatase for CheY
creator: Zilong WANG, Yi ZHENG
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).
mutable notes: how to control the proper expression of cheZ
mutable provenance: genome of E. Coli BL21 plys strain
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fwd primer
sequence annotation: rev primer
sequence annotation: TGA to TAA
sequence annotation: terminator
sequence annotation: start
sequence annotation: CheZ
sequence: https://synbiohub.org/public/igem/BBa_K629003_sequence/1

fwd primer
type: SequenceAnnotation
persistent identity: annotation2154421
display id: annotation2154421
version: 1
top level: cheZ
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1
type: Range
persistent identity: range2154421
display id: range2154421
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 20
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2154423
display id: annotation2154423
version: 1
top level: cheZ
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1
type: Range
persistent identity: range2154423
display id: range2154423
version: 1
direction: direction/forward
top level: cheZ
start: 624
end: 645
orientation: inline

TGA to TAA
type: SequenceAnnotation
persistent identity: annotation2155668
display id: annotation2155668
version: 1
top level: cheZ
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1
type: Range
persistent identity: range2155668
display id: range2155668
version: 1
direction: direction/forward
top level: cheZ
start: 643
end: 645
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2154424
display id: annotation2154424
version: 1
top level: cheZ
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1
type: Range
persistent identity: range2154424
display id: range2154424
version: 1
direction: direction/forward
top level: cheZ
start: 643
end: 645
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2154422
display id: annotation2154422
version: 1
top level: cheZ
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1
type: Range
persistent identity: range2154422
display id: range2154422
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 3
orientation: inline

CheZ
type: SequenceAnnotation
persistent identity: annotation2154425
display id: annotation2154425
version: 1
top level: cheZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1
type: Range
persistent identity: range2154425
display id: range2154425
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 645
orientation: inline

BBa_K792009
type: ComponentDefinition
persistent identity: BBa K792009
display id: BBa_K792009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast exportable His-rich peptide w/enhanced import (1)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This composite device produces and exports a Histidine rich peptide, that is import enhanced (thanks to a trojan peptide). The high level structure of the device is: * Kozak consensus sequence for initiation of translation * Signal peptide that targets the product of the gene for secretion * Trojan peptide, to increase internalization in target cell * Payload: this is the exported amino acid rich domain of the protein
mutable notes: Composite part
mutable provenance: Composite part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K792002
component: BBa_K792003
component: BBa_K792001
component: BBa_K792005
sequence annotation: BBa_K792001
sequence annotation: BBa_K792002
sequence annotation: BBa_K792005
sequence annotation: BBa_K792003
sequence: https://synbiohub.org/public/igem/BBa_K792009_sequence/1

BBa_K792002
type: Component
persistent identity: component2196482
display id: component2196482
version: 1
top level: BBa_K792009
access: public
definition: https://synbiohub.org/public/igem/BBa_K792002/1

BBa_K792003
type: Component
persistent identity: component2196484
display id: component2196484
version: 1
top level: BBa_K792009
access: public
definition: https://synbiohub.org/public/igem/BBa_K792003/1

BBa_K792001
type: Component
persistent identity: component2196481
display id: component2196481
version: 1
top level: BBa_K792009
access: public
definition: https://synbiohub.org/public/igem/BBa_K792001/1

BBa_K792005
type: Component
persistent identity: component2196486
display id: component2196486
version: 1
top level: BBa_K792009
access: public
definition: https://synbiohub.org/public/igem/BBa_K792005/1

BBa_K792001
type: SequenceAnnotation
persistent identity: annotation2196481
display id: annotation2196481
version: 1
top level: BBa_K792009
location: https://synbiohub.org/public/igem/BBa_K792009/annotation2196481/range2196481/1
component: BBa_K792001

https://synbiohub.org/public/igem/BBa_K792009/annotation2196481/range2196481/1
type: Range
persistent identity: range2196481
display id: range2196481
version: 1
direction: direction/reverse
top level: BBa_K792009
start: 1
end: 21
orientation: inline

BBa_K792002
type: SequenceAnnotation
persistent identity: annotation2196482
display id: annotation2196482
version: 1
top level: BBa_K792009
location: https://synbiohub.org/public/igem/BBa_K792009/annotation2196482/range2196482/1
component: BBa_K792002

https://synbiohub.org/public/igem/BBa_K792009/annotation2196482/range2196482/1
type: Range
persistent identity: range2196482
display id: range2196482
version: 1
direction: direction/reverse
top level: BBa_K792009
start: 22
end: 75
orientation: inline

BBa_K792005
type: SequenceAnnotation
persistent identity: annotation2196486
display id: annotation2196486
version: 1
top level: BBa_K792009
location: https://synbiohub.org/public/igem/BBa_K792009/annotation2196486/range2196486/1
component: BBa_K792005

https://synbiohub.org/public/igem/BBa_K792009/annotation2196486/range2196486/1
type: Range
persistent identity: range2196486
display id: range2196486
version: 1
direction: direction/reverse
top level: BBa_K792009
start: 115
end: 162
orientation: inline

BBa_K792003
type: SequenceAnnotation
persistent identity: annotation2196484
display id: annotation2196484
version: 1
top level: BBa_K792009
location: https://synbiohub.org/public/igem/BBa_K792009/annotation2196484/range2196484/1
component: BBa_K792003

https://synbiohub.org/public/igem/BBa_K792009/annotation2196484/range2196484/1
type: Range
persistent identity: range2196484
display id: range2196484
version: 1
direction: direction/reverse
top level: BBa_K792009
start: 76
end: 114
orientation: inline

BBa_K823010
type: ComponentDefinition
persistent identity: BBa K823010
display id: BBa_K823010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23113
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23113 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: J23113
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823010_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182536
display id: annotation2182536
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1
type: Range
persistent identity: range2182536
display id: range2182536
version: 1
direction: direction/forward
top level: BBa_K823010
start: 24
end: 29
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2182534
display id: annotation2182534
version: 1
top level: BBa_K823010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1
type: Range
persistent identity: range2182534
display id: range2182534
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182535
display id: annotation2182535
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1
type: Range
persistent identity: range2182535
display id: range2182535
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 6
orientation: inline

8BE
type: ComponentDefinition
persistent identity: BBa K590056
display id: BBa_K590056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RrD_C70K-PSB1C3-High constitutive
creator: Lei Zheng, Ben Mo, Elaine Lai, Juhye An
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Plasmid that consists of Acyl-ACP Reductase rbs mutant Synechococcus elongatus PCC7942 Aldehyde Decarbonylase in PSB1C3 high consititutive vector for alkane production
mutable notes: TBD
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K590039
component: BBa_K314100
component: BBa_K590032
sequence annotation: BBa_K590032
sequence annotation: BBa_K314100
sequence annotation: BBa_B0034
sequence annotation: BBa_K590039
sequence: https://synbiohub.org/public/igem/BBa_K590056_sequence/1

BBa_B0034
type: Component
persistent identity: component2129354
display id: component2129354
version: 1
top level: 8BE
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K590039
type: Component
persistent identity: component2129355
display id: component2129355
version: 1
top level: 8BE
access: public
definition: https://synbiohub.org/public/igem/BBa_K590039/1

BBa_K314100
type: Component
persistent identity: component2129351
display id: component2129351
version: 1
top level: 8BE
access: public
definition: BBa_K314100

BBa_K590032
type: Component
persistent identity: component2129352
display id: component2129352
version: 1
top level: 8BE
access: public
definition: https://synbiohub.org/public/igem/BBa_K590032/1

BBa_K590032
type: SequenceAnnotation
persistent identity: annotation2129352
display id: annotation2129352
version: 1
top level: 8BE
location: https://synbiohub.org/public/igem/BBa_K590056/annotation2129352/range2129352/1
component: BBa_K590032

https://synbiohub.org/public/igem/BBa_K590056/annotation2129352/range2129352/1
type: Range
persistent identity: range2129352
display id: range2129352
version: 1
direction: direction/reverse
top level: 8BE
start: 525
end: 1610
orientation: inline

BBa_K314100
type: SequenceAnnotation
persistent identity: annotation2129351
display id: annotation2129351
version: 1
top level: 8BE
location: https://synbiohub.org/public/igem/BBa_K590056/annotation2129351/range2129351/1
component: BBa_K314100

https://synbiohub.org/public/igem/BBa_K590056/annotation2129351/range2129351/1
type: Range
persistent identity: range2129351
display id: range2129351
version: 1
direction: direction/reverse
top level: 8BE
start: 1
end: 518
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2129354
display id: annotation2129354
version: 1
top level: 8BE
location: https://synbiohub.org/public/igem/BBa_K590056/annotation2129354/range2129354/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K590056/annotation2129354/range2129354/1
type: Range
persistent identity: range2129354
display id: range2129354
version: 1
direction: direction/reverse
top level: 8BE
start: 1619
end: 1630
orientation: inline

BBa_K590039
type: SequenceAnnotation
persistent identity: annotation2129355
display id: annotation2129355
version: 1
top level: 8BE
location: https://synbiohub.org/public/igem/BBa_K590056/annotation2129355/range2129355/1
component: BBa_K590039

https://synbiohub.org/public/igem/BBa_K590056/annotation2129355/range2129355/1
type: Range
persistent identity: range2129355
display id: range2129355
version: 1
direction: direction/reverse
top level: 8BE
start: 1637
end: 2392
orientation: inline

BBa_K575011
type: ComponentDefinition
persistent identity: BBa K575011
display id: BBa_K575011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP
creator: Kristin Palarz
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR/PAI1 inducable promoter with RBS (B0034) and RFP.
mutable notes: Need to ensure no leaky transcription.
mutable provenance: From BioBrick parts registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0079
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_R0079
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K575011_sequence/1

BBa_R0079
type: Component
persistent identity: component2247824
display id: component2247824
version: 1
top level: BBa_K575011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0034
type: Component
persistent identity: component2247826
display id: component2247826
version: 1
top level: BBa_K575011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2247829
display id: component2247829
version: 1
top level: BBa_K575011
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247829
display id: annotation2247829
version: 1
top level: BBa_K575011
location: https://synbiohub.org/public/igem/BBa_K575011/annotation2247829/range2247829/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K575011/annotation2247829/range2247829/1
type: Range
persistent identity: range2247829
display id: range2247829
version: 1
direction: direction/reverse
top level: BBa_K575011
start: 184
end: 889
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2247824
display id: annotation2247824
version: 1
top level: BBa_K575011
location: https://synbiohub.org/public/igem/BBa_K575011/annotation2247824/range2247824/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K575011/annotation2247824/range2247824/1
type: Range
persistent identity: range2247824
display id: range2247824
version: 1
direction: direction/reverse
top level: BBa_K575011
start: 1
end: 157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2247826
display id: annotation2247826
version: 1
top level: BBa_K575011
location: https://synbiohub.org/public/igem/BBa_K575011/annotation2247826/range2247826/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K575011/annotation2247826/range2247826/1
type: Range
persistent identity: range2247826
display id: range2247826
version: 1
direction: direction/reverse
top level: BBa_K575011
start: 166
end: 177
orientation: inline

BBa_K575032
type: ComponentDefinition
persistent identity: BBa K575032
display id: BBa_K575032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive Promoter + RBS (B0030) + RhlR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Continuous expression of RhlR combined with receptor with RhlP.
mutable notes: put the constitutive promoter after the production of RhlR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575006
component: BBa_K575012
sequence annotation: BBa_K575012
sequence annotation: BBa_K575006
sequence: https://synbiohub.org/public/igem/BBa_K575032_sequence/1

BBa_K575006
type: Component
persistent identity: component2138344
display id: component2138344
version: 1
top level: BBa_K575032
access: public
definition: https://synbiohub.org/public/igem/BBa_K575006/1

BBa_K575012
type: Component
persistent identity: component2138338
display id: component2138338
version: 1
top level: BBa_K575032
access: public
definition: BBa_K575012

BBa_K575012
type: SequenceAnnotation
persistent identity: annotation2138338
display id: annotation2138338
version: 1
top level: BBa_K575032
location: https://synbiohub.org/public/igem/BBa_K575032/annotation2138338/range2138338/1
component: BBa_K575012

https://synbiohub.org/public/igem/BBa_K575032/annotation2138338/range2138338/1
type: Range
persistent identity: range2138338
display id: range2138338
version: 1
direction: direction/reverse
top level: BBa_K575032
start: 1
end: 802
orientation: inline

BBa_K575006
type: SequenceAnnotation
persistent identity: annotation2138344
display id: annotation2138344
version: 1
top level: BBa_K575032
location: https://synbiohub.org/public/igem/BBa_K575032/annotation2138344/range2138344/1
component: BBa_K575006

https://synbiohub.org/public/igem/BBa_K575032/annotation2138344/range2138344/1
type: Range
persistent identity: range2138344
display id: range2138344
version: 1
direction: direction/reverse
top level: BBa_K575032
start: 811
end: 1603
orientation: inline

BBa_K606013
type: ComponentDefinition
persistent identity: BBa K606013
display id: BBa_K606013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpoVG RFPmCherry double terminator. Translational unit for B. Subtilis
creator: Cyrille Pauthenier
created: 2011-07-06T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a translational unit for expressing the RFP in B. Subtilis. The RBS used is SpoVG (K143021),
mutable notes: In pSB1K3
mutable provenance: This part is the fusion of the biobricks K143021 E1010 and B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K143021
component: BBa_K606012
sequence annotation: BBa_K143021
sequence annotation: BBa_K606012
sequence: https://synbiohub.org/public/igem/BBa_K606013_sequence/1

BBa_K143021
type: Component
persistent identity: component2250502
display id: component2250502
version: 1
top level: BBa_K606013
access: public
definition: https://synbiohub.org/public/igem/BBa_K143021/1

BBa_K606012
type: Component
persistent identity: component2250513
display id: component2250513
version: 1
top level: BBa_K606013
access: public
definition: https://synbiohub.org/public/igem/BBa_K606012/1

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2250502
display id: annotation2250502
version: 1
top level: BBa_K606013
location: https://synbiohub.org/public/igem/BBa_K606013/annotation2250502/range2250502/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K606013/annotation2250502/range2250502/1
type: Range
persistent identity: range2250502
display id: range2250502
version: 1
direction: direction/reverse
top level: BBa_K606013
start: 1
end: 12
orientation: inline

BBa_K606012
type: SequenceAnnotation
persistent identity: annotation2250513
display id: annotation2250513
version: 1
top level: BBa_K606013
location: https://synbiohub.org/public/igem/BBa_K606013/annotation2250513/range2250513/1
component: BBa_K606012

https://synbiohub.org/public/igem/BBa_K606013/annotation2250513/range2250513/1
type: Range
persistent identity: range2250513
display id: range2250513
version: 1
direction: direction/reverse
top level: BBa_K606013
start: 19
end: 861
orientation: inline

BBa_K607038
type: ComponentDefinition
persistent identity: BBa K607038
display id: BBa_K607038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prm modified promoter generating GFP
creator: Jakub Radzikowski
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _779_
m user id: 0
owner id: 8814
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Modified lamdba Prm promoter (repressed by 434 cI) generating GFP. Part used for measurements.
mutable notes: none
mutable provenance: distribution 2011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I12006
component: BBa_E0840
sequence annotation: BBa_E0840
sequence annotation: BBa_I12006
sequence: https://synbiohub.org/public/igem/BBa_K607038_sequence/1

BBa_I12006
type: Component
persistent identity: component2136307
display id: component2136307
version: 1
top level: BBa_K607038
access: public
definition: https://synbiohub.org/public/igem/BBa_I12006/1

BBa_E0840
type: Component
persistent identity: component2136318
display id: component2136318
version: 1
top level: BBa_K607038
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2136318
display id: annotation2136318
version: 1
top level: BBa_K607038
location: https://synbiohub.org/public/igem/BBa_K607038/annotation2136318/range2136318/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K607038/annotation2136318/range2136318/1
type: Range
persistent identity: range2136318
display id: range2136318
version: 1
direction: direction/reverse
top level: BBa_K607038
start: 91
end: 968
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation2136307
display id: annotation2136307
version: 1
top level: BBa_K607038
location: https://synbiohub.org/public/igem/BBa_K607038/annotation2136307/range2136307/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_K607038/annotation2136307/range2136307/1
type: Range
persistent identity: range2136307
display id: range2136307
version: 1
direction: direction/reverse
top level: BBa_K607038
start: 1
end: 82
orientation: inline

BBa_K541505
type: ComponentDefinition
persistent identity: BBa K541505
display id: BBa_K541505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: limulus anti-LPS factor (LALF)
creator: Fazilet Guler
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _708_
m user id: 0
owner id: 8463
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge- shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.
mutable notes: optimized for both e.coli and b.subtilis
mutable provenance: horseshoe crab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: LALF
sequence: https://synbiohub.org/public/igem/BBa_K541505_sequence/1

LALF
type: SequenceAnnotation
persistent identity: annotation2123467
display id: annotation2123467
version: 1
top level: BBa_K541505
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K541505/annotation2123467/range2123467/1

https://synbiohub.org/public/igem/BBa_K541505/annotation2123467/range2123467/1
type: Range
persistent identity: range2123467
display id: range2123467
version: 1
direction: direction/forward
top level: BBa_K541505
start: 1
end: 312
orientation: inline

BBa_K523020
type: ComponentDefinition
persistent identity: BBa K523020
display id: BBa_K523020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac + INP-bglX
creator: Mun Ching Lee, Sylvia Ispasanie
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: Later
mutable provenance: Later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Spacer
sequence annotation: Spacer (Linker)
sequence annotation: -35
sequence annotation: LacZ
sequence annotation: INP-bglX fusion
sequence annotation: -10
sequence annotation: rbs
sequence annotation: Plac - LacZ
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K523020_sequence/1

Spacer
type: SequenceAnnotation
persistent identity: annotation2128971
display id: annotation2128971
version: 1
top level: BBa_K523020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2128971/range2128971/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2128971/range2128971/1
type: Range
persistent identity: range2128971
display id: range2128971
version: 1
direction: direction/forward
top level: BBa_K523020
start: 597
end: 625
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2128974
display id: annotation2128974
version: 1
top level: BBa_K523020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2128974/range2128974/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2128974/range2128974/1
type: Range
persistent identity: range2128974
display id: range2128974
version: 1
direction: direction/forward
top level: BBa_K523020
start: 3911
end: 3938
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2128968
display id: annotation2128968
version: 1
top level: BBa_K523020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2128968/range2128968/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2128968/range2128968/1
type: Range
persistent identity: range2128968
display id: range2128968
version: 1
direction: direction/forward
top level: BBa_K523020
start: 1
end: 32
orientation: inline

Spacer (Linker)
type: SequenceAnnotation
persistent identity: annotation2147348
display id: annotation2147348
version: 1
top level: BBa_K523020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2147348/range2147348/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2147348/range2147348/1
type: Range
persistent identity: range2147348
display id: range2147348
version: 1
direction: direction/forward
top level: BBa_K523020
start: 1565
end: 1600
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2129012
display id: annotation2129012
version: 1
top level: BBa_K523020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2129012/range2129012/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2129012/range2129012/1
type: Range
persistent identity: range2129012
display id: range2129012
version: 1
direction: direction/forward
top level: BBa_K523020
start: 286
end: 291
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2128970
display id: annotation2128970
version: 1
top level: BBa_K523020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2128970/range2128970/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2128970/range2128970/1
type: Range
persistent identity: range2128970
display id: range2128970
version: 1
direction: direction/forward
top level: BBa_K523020
start: 359
end: 589
orientation: inline

INP-bglX fusion
type: SequenceAnnotation
persistent identity: annotation2128972
display id: annotation2128972
version: 1
top level: BBa_K523020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2128972/range2128972/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2128972/range2128972/1
type: Range
persistent identity: range2128972
display id: range2128972
version: 1
direction: direction/forward
top level: BBa_K523020
start: 623
end: 3922
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2129013
display id: annotation2129013
version: 1
top level: BBa_K523020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2129013/range2129013/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2129013/range2129013/1
type: Range
persistent identity: range2129013
display id: range2129013
version: 1
direction: direction/forward
top level: BBa_K523020
start: 309
end: 314
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129014
display id: annotation2129014
version: 1
top level: BBa_K523020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2129014/range2129014/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2129014/range2129014/1
type: Range
persistent identity: range2129014
display id: range2129014
version: 1
direction: direction/forward
top level: BBa_K523020
start: 348
end: 351
orientation: inline

Plac - LacZ
type: SequenceAnnotation
persistent identity: annotation2128969
display id: annotation2128969
version: 1
top level: BBa_K523020
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2128969/range2128969/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2128969/range2128969/1
type: Range
persistent identity: range2128969
display id: range2128969
version: 1
direction: direction/forward
top level: BBa_K523020
start: 38
end: 589
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2128973
display id: annotation2128973
version: 1
top level: BBa_K523020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2128973/range2128973/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2128973/range2128973/1
type: Range
persistent identity: range2128973
display id: range2128973
version: 1
direction: direction/forward
top level: BBa_K523020
start: 611
end: 614
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2128975
display id: annotation2128975
version: 1
top level: BBa_K523020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K523020/annotation2128975/range2128975/1

https://synbiohub.org/public/igem/BBa_K523020/annotation2128975/range2128975/1
type: Range
persistent identity: range2128975
display id: range2128975
version: 1
direction: direction/forward
top level: BBa_K523020
start: 3920
end: 3920
orientation: inline

BBa_K590030
type: ComponentDefinition
persistent identity: BBa K590030
display id: BBa_K590030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DrR-FabH2-PSB1C3-High constitutive
creator: Matthew Harger, Lei Zheng
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Aldehyde Decarbonylase rbs Acyl-ACP Reductase FabH2 in PSB1C3 high consititutive vector for branched alkane production
mutable notes: None
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K590025
component: BBa_K590034
component: BBa_B0034
sequence annotation: BBa_K590025
sequence annotation: BBa_B0034
sequence annotation: BBa_K590034
sequence: https://synbiohub.org/public/igem/BBa_K590030_sequence/1

BBa_K590025
type: Component
persistent identity: component2129213
display id: component2129213
version: 1
top level: BBa_K590030
access: public
definition: https://synbiohub.org/public/igem/BBa_K590025/1

BBa_K590034
type: Component
persistent identity: component2129216
display id: component2129216
version: 1
top level: BBa_K590030
access: public
definition: FabH2

BBa_B0034
type: Component
persistent identity: component2129215
display id: component2129215
version: 1
top level: BBa_K590030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K590025
type: SequenceAnnotation
persistent identity: annotation2129213
display id: annotation2129213
version: 1
top level: BBa_K590030
location: https://synbiohub.org/public/igem/BBa_K590030/annotation2129213/range2129213/1
component: BBa_K590025

https://synbiohub.org/public/igem/BBa_K590030/annotation2129213/range2129213/1
type: Range
persistent identity: range2129213
display id: range2129213
version: 1
direction: direction/reverse
top level: BBa_K590030
start: 1
end: 2392
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2129215
display id: annotation2129215
version: 1
top level: BBa_K590030
location: https://synbiohub.org/public/igem/BBa_K590030/annotation2129215/range2129215/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K590030/annotation2129215/range2129215/1
type: Range
persistent identity: range2129215
display id: range2129215
version: 1
direction: direction/reverse
top level: BBa_K590030
start: 2401
end: 2412
orientation: inline

BBa_K590034
type: SequenceAnnotation
persistent identity: annotation2129216
display id: annotation2129216
version: 1
top level: BBa_K590030
location: https://synbiohub.org/public/igem/BBa_K590030/annotation2129216/range2129216/1
component: BBa_K590034

https://synbiohub.org/public/igem/BBa_K590030/annotation2129216/range2129216/1
type: Range
persistent identity: range2129216
display id: range2129216
version: 1
direction: direction/reverse
top level: BBa_K590030
start: 2419
end: 3441
orientation: inline

BBa_K575024
type: ComponentDefinition
persistent identity: BBa K575024
display id: BBa_K575024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR
creator: Kristin Palarz
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _746_
m user id: 0
owner id: 8735
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Continuous expression of LasR combined with receptor
mutable notes: put the constitutive promoter after the production of LasR
mutable provenance: from registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K575008
component: BBa_K575004
sequence annotation: BBa_K575004
sequence annotation: BBa_K575008
sequence: https://synbiohub.org/public/igem/BBa_K575024_sequence/1

BBa_K575008
type: Component
persistent identity: component2138239
display id: component2138239
version: 1
top level: BBa_K575024
access: public
definition: BBa_K575008

BBa_K575004
type: Component
persistent identity: component2138245
display id: component2138245
version: 1
top level: BBa_K575024
access: public
definition: https://synbiohub.org/public/igem/BBa_K575004/1

BBa_K575004
type: SequenceAnnotation
persistent identity: annotation2138245
display id: annotation2138245
version: 1
top level: BBa_K575024
location: https://synbiohub.org/public/igem/BBa_K575024/annotation2138245/range2138245/1
component: BBa_K575004

https://synbiohub.org/public/igem/BBa_K575024/annotation2138245/range2138245/1
type: Range
persistent identity: range2138245
display id: range2138245
version: 1
direction: direction/reverse
top level: BBa_K575024
start: 915
end: 1701
orientation: inline

BBa_K575008
type: SequenceAnnotation
persistent identity: annotation2138239
display id: annotation2138239
version: 1
top level: BBa_K575024
location: https://synbiohub.org/public/igem/BBa_K575024/annotation2138239/range2138239/1
component: BBa_K575008

https://synbiohub.org/public/igem/BBa_K575024/annotation2138239/range2138239/1
type: Range
persistent identity: range2138239
display id: range2138239
version: 1
direction: direction/reverse
top level: BBa_K575024
start: 1
end: 906
orientation: inline

ChiPm-lacZ
type: ComponentDefinition
persistent identity: BBa K564013
display id: BBa_K564013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Upstream mutated chitoporin part fused with lacZ
creator: Anna Lewinska
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: true
experience: experience/None
group u list: _732_
m user id: 0
owner id: 8333
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.In this biobrick we altered the binding site for ChiP regulator ??? ChiX. It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.
mutable notes: There are no design considerations.
mutable provenance: The subparts were prepared: ChiP upstream part from Escherichia Coli W3110 genomic DNA by PCR and lacZ from part BBa_I732005 present in part registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_I732005
component: BBa_B0012
component: BBa_K564015
sequence annotation: BBa_B0010
sequence annotation: BBa_I732005
sequence annotation: BBa_K564015
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K564013_sequence/1

BBa_B0010
type: Component
persistent identity: component2136028
display id: component2136028
version: 1
top level: ChiPm-lacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I732005
type: Component
persistent identity: component2136027
display id: component2136027
version: 1
top level: ChiPm-lacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_I732005/1

BBa_B0012
type: Component
persistent identity: component2136030
display id: component2136030
version: 1
top level: ChiPm-lacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K564015
type: Component
persistent identity: component2136022
display id: component2136022
version: 1
top level: ChiPm-lacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_K564015/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2136028
display id: annotation2136028
version: 1
top level: ChiPm-lacZ
location: https://synbiohub.org/public/igem/BBa_K564013/annotation2136028/range2136028/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K564013/annotation2136028/range2136028/1
type: Range
persistent identity: range2136028
display id: range2136028
version: 1
direction: direction/forward
top level: ChiPm-lacZ
start: 3689
end: 3768
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2136027
display id: annotation2136027
version: 1
top level: ChiPm-lacZ
location: https://synbiohub.org/public/igem/BBa_K564013/annotation2136027/range2136027/1
component: BBa_I732005

https://synbiohub.org/public/igem/BBa_K564013/annotation2136027/range2136027/1
type: Range
persistent identity: range2136027
display id: range2136027
version: 1
direction: direction/reverse
top level: ChiPm-lacZ
start: 606
end: 3680
orientation: inline

BBa_K564015
type: SequenceAnnotation
persistent identity: annotation2136022
display id: annotation2136022
version: 1
top level: ChiPm-lacZ
location: https://synbiohub.org/public/igem/BBa_K564013/annotation2136022/range2136022/1
component: BBa_K564015

https://synbiohub.org/public/igem/BBa_K564013/annotation2136022/range2136022/1
type: Range
persistent identity: range2136022
display id: range2136022
version: 1
direction: direction/reverse
top level: ChiPm-lacZ
start: 1
end: 599
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2136030
display id: annotation2136030
version: 1
top level: ChiPm-lacZ
location: https://synbiohub.org/public/igem/BBa_K564013/annotation2136030/range2136030/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K564013/annotation2136030/range2136030/1
type: Range
persistent identity: range2136030
display id: range2136030
version: 1
direction: direction/forward
top level: ChiPm-lacZ
start: 3777
end: 3817
orientation: inline

BBa_K733017
type: ComponentDefinition
persistent identity: BBa K733017
display id: BBa_K733017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg+RBS+ybdN+Bmp2+terminator
creator: Chris Yu Lai Cheong; LI Yiming
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K733015
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K733015
sequence: https://synbiohub.org/public/igem/BBa_K733017_sequence/1

BBa_K733015
type: Component
persistent identity: component2183834
display id: component2183834
version: 1
top level: BBa_K733017
access: public
definition: https://synbiohub.org/public/igem/BBa_K733015/1

BBa_B0015
type: Component
persistent identity: component2183841
display id: component2183841
version: 1
top level: BBa_K733017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2183841
display id: annotation2183841
version: 1
top level: BBa_K733017
location: https://synbiohub.org/public/igem/BBa_K733017/annotation2183841/range2183841/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K733017/annotation2183841/range2183841/1
type: Range
persistent identity: range2183841
display id: range2183841
version: 1
direction: direction/reverse
top level: BBa_K733017
start: 565
end: 693
orientation: inline

BBa_K733015
type: SequenceAnnotation
persistent identity: annotation2183834
display id: annotation2183834
version: 1
top level: BBa_K733017
location: https://synbiohub.org/public/igem/BBa_K733017/annotation2183834/range2183834/1
component: BBa_K733015

https://synbiohub.org/public/igem/BBa_K733017/annotation2183834/range2183834/1
type: Range
persistent identity: range2183834
display id: range2183834
version: 1
direction: direction/reverse
top level: BBa_K733017
start: 1
end: 556
orientation: inline

BBa_K817018
type: ComponentDefinition
persistent identity: BBa K817018
display id: BBa_K817018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gamma-delta resolvase cassette
creator: Shan Chi Hsieh
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1075_
m user id: 0
owner id: 13960
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: These cassette is encodes an autoregulated resolvase(serine type recombinase)from E.coli F plasmid transposon tn1000(tn3 family). Its promoter region consists of 3 sub-sites(res site) and can process recombination. This cassette can resolve multimer that formed during replicative transposition, so it can also resolve plasmid multimer providing plasmid stability by avoiding dimer catastrophe. For this reason, it is multimer resolution system(MRS) that provide the same function as E.coli chromosome XerCD/dif but acts independent of cell cycle and may have higher efficiency on plasmids compared with slow XerCD system.
mutable notes: The native sequence have two PstI site, these sites have been cleared by mutagenesis.
mutable provenance: E.coli F plasmid transposon tn1000(tn3 family)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: recombination site
sequence annotation: tnpX
sequence annotation: PstI
sequence annotation: resII-R
sequence annotation: resI-L
sequence annotation: resIII-R
sequence annotation: tnpA
sequence annotation: tnpR
sequence annotation: resI-R
sequence annotation: resIII-L
sequence annotation: resII-L
sequence: https://synbiohub.org/public/igem/BBa_K817018_sequence/1

recombination site
type: SequenceAnnotation
persistent identity: annotation2187452
display id: annotation2187452
version: 1
top level: BBa_K817018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187452/range2187452/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187452/range2187452/1
type: Range
persistent identity: range2187452
display id: range2187452
version: 1
direction: direction/forward
top level: BBa_K817018
start: 169
end: 169
orientation: inline

tnpX
type: SequenceAnnotation
persistent identity: annotation2187437
display id: annotation2187437
version: 1
top level: BBa_K817018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187437/range2187437/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187437/range2187437/1
type: Range
persistent identity: range2187437
display id: range2187437
version: 1
direction: direction/forward
top level: BBa_K817018
start: 841
end: 888
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2187451
display id: annotation2187451
version: 1
top level: BBa_K817018
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187451/range2187451/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187451/range2187451/1
type: Range
persistent identity: range2187451
display id: range2187451
version: 1
direction: direction/forward
top level: BBa_K817018
start: 612
end: 612
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2187450
display id: annotation2187450
version: 1
top level: BBa_K817018
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187450/range2187450/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187450/range2187450/1
type: Range
persistent identity: range2187450
display id: range2187450
version: 1
direction: direction/forward
top level: BBa_K817018
start: 53
end: 53
orientation: inline

resII-R
type: SequenceAnnotation
persistent identity: annotation2187447
display id: annotation2187447
version: 1
top level: BBa_K817018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187447/range2187447/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187447/range2187447/1
type: Range
persistent identity: range2187447
display id: range2187447
version: 1
direction: direction/reverse
top level: BBa_K817018
start: 230
end: 238
orientation: inline

resI-L
type: SequenceAnnotation
persistent identity: annotation2187444
display id: annotation2187444
version: 1
top level: BBa_K817018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187444/range2187444/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187444/range2187444/1
type: Range
persistent identity: range2187444
display id: range2187444
version: 1
direction: direction/forward
top level: BBa_K817018
start: 156
end: 163
orientation: inline

resIII-R
type: SequenceAnnotation
persistent identity: annotation2187449
display id: annotation2187449
version: 1
top level: BBa_K817018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187449/range2187449/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187449/range2187449/1
type: Range
persistent identity: range2187449
display id: range2187449
version: 1
direction: direction/reverse
top level: BBa_K817018
start: 260
end: 268
orientation: inline

tnpA
type: SequenceAnnotation
persistent identity: annotation2187435
display id: annotation2187435
version: 1
top level: BBa_K817018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187435/range2187435/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187435/range2187435/1
type: Range
persistent identity: range2187435
display id: range2187435
version: 1
direction: direction/reverse
top level: BBa_K817018
start: 1
end: 110
orientation: inline

tnpR
type: SequenceAnnotation
persistent identity: annotation2187436
display id: annotation2187436
version: 1
top level: BBa_K817018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187436/range2187436/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187436/range2187436/1
type: Range
persistent identity: range2187436
display id: range2187436
version: 1
direction: direction/forward
top level: BBa_K817018
start: 274
end: 825
orientation: inline

resI-R
type: SequenceAnnotation
persistent identity: annotation2187445
display id: annotation2187445
version: 1
top level: BBa_K817018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187445/range2187445/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187445/range2187445/1
type: Range
persistent identity: range2187445
display id: range2187445
version: 1
direction: direction/reverse
top level: BBa_K817018
start: 176
end: 184
orientation: inline

resIII-L
type: SequenceAnnotation
persistent identity: annotation2187448
display id: annotation2187448
version: 1
top level: BBa_K817018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187448/range2187448/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187448/range2187448/1
type: Range
persistent identity: range2187448
display id: range2187448
version: 1
direction: direction/forward
top level: BBa_K817018
start: 244
end: 252
orientation: inline

resII-L
type: SequenceAnnotation
persistent identity: annotation2187446
display id: annotation2187446
version: 1
top level: BBa_K817018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K817018/annotation2187446/range2187446/1

https://synbiohub.org/public/igem/BBa_K817018/annotation2187446/range2187446/1
type: Range
persistent identity: range2187446
display id: range2187446
version: 1
direction: direction/forward
top level: BBa_K817018
start: 206
end: 214
orientation: inline

BBa_K325209
type: ComponentDefinition
persistent identity: BBa K325209
display id: BBa_K325209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green Firefly Luciferase and LRE (under pBAD) L. Cruciata (E. coli optimised)
creator: Anja Hohmann and Will Handley
created: 2010-09-16T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the lux operon from V. fischeri expressed under a pBAD promoter. It emits light when induced by arabinose. luxCDE create a substrate which the luciferase complex of luxA and luxB uses to emit light at a blue wavelength.
mutable notes: Removal of existing lux promoter, and replacement with pBAD
mutable provenance: From pHK555 provided by James Slock ( http://departments.kings.edu/biology/lux/bacterial.html )
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: double stop codon
sequence annotation: PC
sequence annotation: AraC
sequence annotation: I0500
sequence annotation: rbs
sequence annotation: BamH1 site
sequence annotation: Luciferin Regenerating Enzyme (L. cruciata)
sequence annotation: Wild Type Luciferase (L. cruciata)
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_K325209_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103106
display id: annotation2103106
version: 1
top level: BBa_K325209
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103106/range2103106/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103106/range2103106/1
type: Range
persistent identity: range2103106
display id: range2103106
version: 1
direction: direction/forward
top level: BBa_K325209
start: 2163
end: 2168
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2103102
display id: annotation2103102
version: 1
top level: BBa_K325209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103102/range2103102/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103102/range2103102/1
type: Range
persistent identity: range2103102
display id: range2103102
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1043
end: 1114
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2103101
display id: annotation2103101
version: 1
top level: BBa_K325209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103101/range2103101/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103101/range2103101/1
type: Range
persistent identity: range2103101
display id: range2103101
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1
end: 1079
orientation: inline

I0500
type: SequenceAnnotation
persistent identity: annotation2103100
display id: annotation2103100
version: 1
top level: BBa_K325209
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103100/range2103100/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103100/range2103100/1
type: Range
persistent identity: range2103100
display id: range2103100
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1
end: 1210
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103108
display id: annotation2103108
version: 1
top level: BBa_K325209
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103108/range2103108/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103108/range2103108/1
type: Range
persistent identity: range2103108
display id: range2103108
version: 1
direction: direction/forward
top level: BBa_K325209
start: 2175
end: 2191
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2103107
display id: annotation2103107
version: 1
top level: BBa_K325209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103107/range2103107/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103107/range2103107/1
type: Range
persistent identity: range2103107
display id: range2103107
version: 1
direction: direction/forward
top level: BBa_K325209
start: 2169
end: 2174
orientation: inline

Luciferin Regenerating Enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103105
display id: annotation2103105
version: 1
top level: BBa_K325209
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103105/range2103105/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103105/range2103105/1
type: Range
persistent identity: range2103105
display id: range2103105
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1236
end: 2162
orientation: inline

Wild Type Luciferase (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103109
display id: annotation2103109
version: 1
top level: BBa_K325209
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103109/range2103109/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103109/range2103109/1
type: Range
persistent identity: range2103109
display id: range2103109
version: 1
direction: direction/forward
top level: BBa_K325209
start: 2192
end: 3835
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103104
display id: annotation2103104
version: 1
top level: BBa_K325209
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103104/range2103104/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103104/range2103104/1
type: Range
persistent identity: range2103104
display id: range2103104
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1219
end: 1235
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2103103
display id: annotation2103103
version: 1
top level: BBa_K325209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103103/range2103103/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103103/range2103103/1
type: Range
persistent identity: range2103103
display id: range2103103
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1080
end: 1210
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103119
display id: annotation2103119
version: 1
top level: BBa_K325209
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103119/range2103119/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103119/range2103119/1
type: Range
persistent identity: range2103119
display id: range2103119
version: 1
direction: direction/forward
top level: BBa_K325209
start: 3836
end: 3841
orientation: inline

BM-CFP
type: ComponentDefinition
persistent identity: BBa K774004
display id: BBa_K774004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420
creator: NRP-UEA-Norwich
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1026_
m user id: 0
owner id: 12400
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Our hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably. The hybrid promoter has been attached to the reporter: enhanced Cyan Fluorescence Protein (eCFP). The hybrid promoter has been characterised by observing expression of flourescent protein, and found to have increased transcription in response to increasing concentrations of potassium nitrate.
mutable notes: The enhanced Cyan Fluorescence Protein (eCFP) was chosen with the eventual aim of attaching it to part BBa_K774002 or BBa_K774003. It was therefore important that the fluorescence peak of eCFP didn't overlap with RFP too much, as eCFP and RFP will be reporting on either part BBa_K774002 or BBa_K774003 at the same time.
mutable provenance: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + BBa_E0420
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0420
component: BBa_K774000
sequence annotation: BBa_K774000
sequence annotation: BBa_E0420
sequence: https://synbiohub.org/public/igem/BBa_K774004_sequence/1

BBa_E0420
type: Component
persistent identity: component2187156
display id: component2187156
version: 1
top level: BM-CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0420/1

BBa_K774000
type: Component
persistent identity: component2187146
display id: component2187146
version: 1
top level: BM-CFP
access: public
definition: B-M

BBa_K774000
type: SequenceAnnotation
persistent identity: annotation2187146
display id: annotation2187146
version: 1
top level: BM-CFP
location: https://synbiohub.org/public/igem/BBa_K774004/annotation2187146/range2187146/1
component: BBa_K774000

https://synbiohub.org/public/igem/BBa_K774004/annotation2187146/range2187146/1
type: Range
persistent identity: range2187146
display id: range2187146
version: 1
direction: direction/reverse
top level: BM-CFP
start: 1
end: 208
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2187156
display id: annotation2187156
version: 1
top level: BM-CFP
location: https://synbiohub.org/public/igem/BBa_K774004/annotation2187156/range2187156/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_K774004/annotation2187156/range2187156/1
type: Range
persistent identity: range2187156
display id: range2187156
version: 1
direction: direction/reverse
top level: BM-CFP
start: 217
end: 1094
orientation: inline