iGEM_2019_Plate2_Well9E
1
iGEM 2019 Distribution Plate 2 Well 9E
iGEM_2019_Plate2_Well23D
1
iGEM 2019 Distribution Plate 2 Well 23D
iGEM_2019_Plate2_Well6C
1
iGEM 2019 Distribution Plate 2 Well 6C
iGEM_2019_Plate2_Well5E
1
iGEM 2019 Distribution Plate 2 Well 5E
iGEM_2019_Plate2_Well14O
1
iGEM 2019 Distribution Plate 2 Well 14O
iGEM_2019_Plate2_Well5N
1
iGEM 2019 Distribution Plate 2 Well 5N
iGEM_2019_Plate2_Well3D
1
iGEM 2019 Distribution Plate 2 Well 3D
iGEM_2019_Plate2_Well6O
1
iGEM 2019 Distribution Plate 2 Well 6O
iGEM_2019_Plate2_Well15J
1
iGEM 2019 Distribution Plate 2 Well 15J
iGEM_2019_Plate2_Well8O
1
iGEM 2019 Distribution Plate 2 Well 8O
iGEM_2019_Plate2_Well23P
1
iGEM 2019 Distribution Plate 2 Well 23P
iGEM_2019_Plate2_Well11A
1
iGEM 2019 Distribution Plate 2 Well 11A
iGEM_2019_Plate2_Well1I
1
iGEM 2019 Distribution Plate 2 Well 1I
iGEM_2019_Plate2_Well11L
1
iGEM 2019 Distribution Plate 2 Well 11L
iGEM_2019_Plate2_Well15I
1
iGEM 2019 Distribution Plate 2 Well 15I
iGEM_2019_Plate2_Well16B
1
iGEM 2019 Distribution Plate 2 Well 16B
iGEM_2019_Plate2_Well12G
1
iGEM 2019 Distribution Plate 2 Well 12G
iGEM_2019_Plate2_Well1M
1
iGEM 2019 Distribution Plate 2 Well 1M
iGEM_2019_Plate2_Well6E
1
iGEM 2019 Distribution Plate 2 Well 6E
iGEM_2019_Plate2_Well1K
1
iGEM 2019 Distribution Plate 2 Well 1K
iGEM_2019_Plate2_Well12E
1
iGEM 2019 Distribution Plate 2 Well 12E
iGEM_2019_Plate2_Well12H
1
iGEM 2019 Distribution Plate 2 Well 12H
iGEM_2019_Plate2_Well5I
1
iGEM 2019 Distribution Plate 2 Well 5I
iGEM_2019_Plate2_Well11B
1
iGEM 2019 Distribution Plate 2 Well 11B
iGEM_2019_Plate2_Well24H
1
iGEM 2019 Distribution Plate 2 Well 24H
iGEM_2019_Plate2_Well6P
1
iGEM 2019 Distribution Plate 2 Well 6P
iGEM_2019_Plate2_Well20K
1
iGEM 2019 Distribution Plate 2 Well 20K
iGEM_2019_Plate2_Well23O
1
iGEM 2019 Distribution Plate 2 Well 23O
iGEM_2019_Plate2_Well17O
1
iGEM 2019 Distribution Plate 2 Well 17O
iGEM_2019_Plate2_Well5C
1
iGEM 2019 Distribution Plate 2 Well 5C
iGEM_2019_Plate2_Well15F
1
iGEM 2019 Distribution Plate 2 Well 15F
iGEM_2019_Plate2_Well22M
1
iGEM 2019 Distribution Plate 2 Well 22M
iGEM_2019_Plate2_Well11M
1
iGEM 2019 Distribution Plate 2 Well 11M
iGEM_2019_Plate2_Well13I
1
iGEM 2019 Distribution Plate 2 Well 13I
iGEM_2019_Plate2_Well11I
1
iGEM 2019 Distribution Plate 2 Well 11I
iGEM_2019_Plate2_Well4K
1
iGEM 2019 Distribution Plate 2 Well 4K
iGEM_2019_Plate2_Well10N
1
iGEM 2019 Distribution Plate 2 Well 10N
iGEM_2019_Plate2_Well10F
1
iGEM 2019 Distribution Plate 2 Well 10F
iGEM_2019_Plate2_Well8J
1
iGEM 2019 Distribution Plate 2 Well 8J
iGEM_2019_Plate2_Well20F
1
iGEM 2019 Distribution Plate 2 Well 20F
iGEM_2019_Plate2_Well5G
1
iGEM 2019 Distribution Plate 2 Well 5G
iGEM_2019_Plate2_Well16A
1
iGEM 2019 Distribution Plate 2 Well 16A
iGEM_2019_Plate2_Well13B
1
iGEM 2019 Distribution Plate 2 Well 13B
iGEM_2019_Plate2_Well18C
1
iGEM 2019 Distribution Plate 2 Well 18C
iGEM_2019_Plate2_Well4M
1
iGEM 2019 Distribution Plate 2 Well 4M
iGEM_2019_Plate2_Well23G
1
iGEM 2019 Distribution Plate 2 Well 23G
iGEM_2019_Plate2_Well12N
1
iGEM 2019 Distribution Plate 2 Well 12N
iGEM_2019_Plate2_Well6K
1
iGEM 2019 Distribution Plate 2 Well 6K
iGEM_2019_Plate2_Well8D
1
iGEM 2019 Distribution Plate 2 Well 8D
iGEM_2019_Plate2_Well5D
1
iGEM 2019 Distribution Plate 2 Well 5D
iGEM_2019_Plate2_Well4J
1
iGEM 2019 Distribution Plate 2 Well 4J
iGEM_2019_Plate2_Well23L
1
iGEM 2019 Distribution Plate 2 Well 23L
iGEM_2019_Plate2_Well20D
1
iGEM 2019 Distribution Plate 2 Well 20D
iGEM_2019_Plate2_Well17M
1
iGEM 2019 Distribution Plate 2 Well 17M
iGEM_2019_Plate2_Well10I
1
iGEM 2019 Distribution Plate 2 Well 10I
iGEM_2019_Plate2_Well20A
1
iGEM 2019 Distribution Plate 2 Well 20A
iGEM_2019_Plate2_Well12K
1
iGEM 2019 Distribution Plate 2 Well 12K
iGEM_2019_Plate2_Well18D
1
iGEM 2019 Distribution Plate 2 Well 18D
iGEM_2019_Plate2_Well12P
1
iGEM 2019 Distribution Plate 2 Well 12P
iGEM_2019_Plate2_Well4O
1
iGEM 2019 Distribution Plate 2 Well 4O
iGEM_2019_Plate2_Well20L
1
iGEM 2019 Distribution Plate 2 Well 20L
iGEM_2019_Plate2_Well1L
1
iGEM 2019 Distribution Plate 2 Well 1L
iGEM_2019_Plate2_Well6N
1
iGEM 2019 Distribution Plate 2 Well 6N
iGEM_2019_Plate2_Well2J
1
iGEM 2019 Distribution Plate 2 Well 2J
iGEM_2019_Plate2_Well2G
1
iGEM 2019 Distribution Plate 2 Well 2G
iGEM_2019_Plate2_Well2M
1
iGEM 2019 Distribution Plate 2 Well 2M
iGEM_2019_Plate2_Well3F
1
iGEM 2019 Distribution Plate 2 Well 3F
iGEM_2019_Plate2_Well12J
1
iGEM 2019 Distribution Plate 2 Well 12J
iGEM_2019_Plate2_Well23H
1
iGEM 2019 Distribution Plate 2 Well 23H
iGEM_2019_Plate2_Well16P
1
iGEM 2019 Distribution Plate 2 Well 16P
iGEM_2019_Plate2_Well8B
1
iGEM 2019 Distribution Plate 2 Well 8B
iGEM_2019_Plate2_Well21B
1
iGEM 2019 Distribution Plate 2 Well 21B
iGEM_2019_Plate2_Well1D
1
iGEM 2019 Distribution Plate 2 Well 1D
iGEM_2019_Plate2_Well1E
1
iGEM 2019 Distribution Plate 2 Well 1E
iGEM_2019_Plate2_Well7G
1
iGEM 2019 Distribution Plate 2 Well 7G
iGEM_2019_Plate2_Well20I
1
iGEM 2019 Distribution Plate 2 Well 20I
iGEM_2019_Plate2_Well3P
1
iGEM 2019 Distribution Plate 2 Well 3P
iGEM_2019_Plate2_Well22A
1
iGEM 2019 Distribution Plate 2 Well 22A
iGEM_2019_Plate2_Well3H
1
iGEM 2019 Distribution Plate 2 Well 3H
iGEM_2019_Plate2_Well12I
1
iGEM 2019 Distribution Plate 2 Well 12I
iGEM_2019_Plate2_Well21H
1
iGEM 2019 Distribution Plate 2 Well 21H
iGEM_2019_Plate2_Well14H
1
iGEM 2019 Distribution Plate 2 Well 14H
iGEM_2019_Plate2_Well9N
1
iGEM 2019 Distribution Plate 2 Well 9N
iGEM_2019_Plate2_Well6L
1
iGEM 2019 Distribution Plate 2 Well 6L
iGEM_2019_Plate2_Well1J
1
iGEM 2019 Distribution Plate 2 Well 1J
iGEM_2019_Plate2_Well22I
1
iGEM 2019 Distribution Plate 2 Well 22I
iGEM_2019_Plate2_Well13E
1
iGEM 2019 Distribution Plate 2 Well 13E
iGEM_2019_Plate2_Well13F
1
iGEM 2019 Distribution Plate 2 Well 13F
iGEM_2019_Plate2_Well14J
1
iGEM 2019 Distribution Plate 2 Well 14J
iGEM_2019_Plate2_Well24F
1
iGEM 2019 Distribution Plate 2 Well 24F
iGEM_2019_Plate2_Well10D
1
iGEM 2019 Distribution Plate 2 Well 10D
iGEM_2019_Plate2_Well4P
1
iGEM 2019 Distribution Plate 2 Well 4P
iGEM_2019_Plate2_Well11D
1
iGEM 2019 Distribution Plate 2 Well 11D
iGEM_2019_Plate2_Well11H
1
iGEM 2019 Distribution Plate 2 Well 11H
iGEM_2019_Plate2_Well18P
1
iGEM 2019 Distribution Plate 2 Well 18P
iGEM_2019_Plate2_Well15G
1
iGEM 2019 Distribution Plate 2 Well 15G
iGEM_2019_Plate2_Well19I
1
iGEM 2019 Distribution Plate 2 Well 19I
iGEM_2019_Plate2_Well12O
1
iGEM 2019 Distribution Plate 2 Well 12O
iGEM_2019_Plate2_Well19G
1
iGEM 2019 Distribution Plate 2 Well 19G
iGEM_2019_Plate2_Well4G
1
iGEM 2019 Distribution Plate 2 Well 4G
iGEM_2019_Plate2_Well5J
1
iGEM 2019 Distribution Plate 2 Well 5J
iGEM_2019_Plate2_Well6D
1
iGEM 2019 Distribution Plate 2 Well 6D
iGEM_2019_Plate2_Well10G
1
iGEM 2019 Distribution Plate 2 Well 10G
iGEM_2019_Plate2_Well15D
1
iGEM 2019 Distribution Plate 2 Well 15D
iGEM_2019_Plate2_Well16C
1
iGEM 2019 Distribution Plate 2 Well 16C
iGEM_2019_Plate2_Well18H
1
iGEM 2019 Distribution Plate 2 Well 18H
iGEM_2019_Plate2_Well13A
1
iGEM 2019 Distribution Plate 2 Well 13A
iGEM_2019_Plate2_Well22L
1
iGEM 2019 Distribution Plate 2 Well 22L
iGEM_2019_Plate2_Well16G
1
iGEM 2019 Distribution Plate 2 Well 16G
iGEM_2019_Plate2_Well17C
1
iGEM 2019 Distribution Plate 2 Well 17C
iGEM_2019_Plate2_Well19N
1
iGEM 2019 Distribution Plate 2 Well 19N
iGEM_2019_Plate2_Well11O
1
iGEM 2019 Distribution Plate 2 Well 11O
iGEM_2019_Plate2_Well2N
1
iGEM 2019 Distribution Plate 2 Well 2N
iGEM_2019_Plate2_Well22J
1
iGEM 2019 Distribution Plate 2 Well 22J
iGEM_2019_Plate2_Well19F
1
iGEM 2019 Distribution Plate 2 Well 19F
iGEM_2019_Plate2_Well19K
1
iGEM 2019 Distribution Plate 2 Well 19K
iGEM_2019_Plate2_Well15C
1
iGEM 2019 Distribution Plate 2 Well 15C
iGEM_2019_Plate2_Well7J
1
iGEM 2019 Distribution Plate 2 Well 7J
iGEM_2019_Plate2_Well2L
1
iGEM 2019 Distribution Plate 2 Well 2L
iGEM_2019_Plate2_Well14F
1
iGEM 2019 Distribution Plate 2 Well 14F
iGEM_2019_Plate2_Well21E
1
iGEM 2019 Distribution Plate 2 Well 21E
iGEM_2019_Plate2_Well6I
1
iGEM 2019 Distribution Plate 2 Well 6I
iGEM_2019_Plate2_Well10J
1
iGEM 2019 Distribution Plate 2 Well 10J
iGEM_2019_Plate2_Well20G
1
iGEM 2019 Distribution Plate 2 Well 20G
iGEM_2019_Plate2_Well4N
1
iGEM 2019 Distribution Plate 2 Well 4N
iGEM_2019_Plate2_Well15O
1
iGEM 2019 Distribution Plate 2 Well 15O
iGEM_2019_Plate2_Well22D
1
iGEM 2019 Distribution Plate 2 Well 22D
iGEM_2019_Plate2_Well9O
1
iGEM 2019 Distribution Plate 2 Well 9O
iGEM_2019_Plate2_Well3K
1
iGEM 2019 Distribution Plate 2 Well 3K
iGEM_2019_Plate2_Well7I
1
iGEM 2019 Distribution Plate 2 Well 7I
iGEM_2019_Plate2_Well14B
1
iGEM 2019 Distribution Plate 2 Well 14B
iGEM_2019_Plate2_Well6B
1
iGEM 2019 Distribution Plate 2 Well 6B
iGEM_2019_Plate2_Well10P
1
iGEM 2019 Distribution Plate 2 Well 10P
iGEM_2019_Plate2_Well18F
1
iGEM 2019 Distribution Plate 2 Well 18F
iGEM_2019_Plate2_Well5B
1
iGEM 2019 Distribution Plate 2 Well 5B
iGEM_2019_Plate2_Well14N
1
iGEM 2019 Distribution Plate 2 Well 14N
iGEM_2019_Plate2_Well15A
1
iGEM 2019 Distribution Plate 2 Well 15A
iGEM_2019_Plate2_Well19P
1
iGEM 2019 Distribution Plate 2 Well 19P
iGEM_2019_Plate2_Well9H
1
iGEM 2019 Distribution Plate 2 Well 9H
iGEM_2019_Plate2_Well16M
1
iGEM 2019 Distribution Plate 2 Well 16M
iGEM_2019_Plate2_Well23C
1
iGEM 2019 Distribution Plate 2 Well 23C
iGEM_2019_Plate2_Well6M
1
iGEM 2019 Distribution Plate 2 Well 6M
iGEM_2019_Plate2_Well5A
1
iGEM 2019 Distribution Plate 2 Well 5A
iGEM_2019_Plate2_Well16O
1
iGEM 2019 Distribution Plate 2 Well 16O
iGEM_2019_Plate2_Well15N
1
iGEM 2019 Distribution Plate 2 Well 15N
iGEM_2019_Plate2_Well22N
1
iGEM 2019 Distribution Plate 2 Well 22N
iGEM_2019_Plate2_Well17B
1
iGEM 2019 Distribution Plate 2 Well 17B
iGEM_2019_Plate2_Well20M
1
iGEM 2019 Distribution Plate 2 Well 20M
iGEM_2019_Plate2_Well14I
1
iGEM 2019 Distribution Plate 2 Well 14I
iGEM_2019_Plate2_Well8H
1
iGEM 2019 Distribution Plate 2 Well 8H
iGEM_2019_Plate2_Well20H
1
iGEM 2019 Distribution Plate 2 Well 20H
iGEM_2019_Plate2_Well14K
1
iGEM 2019 Distribution Plate 2 Well 14K
iGEM_2019_Plate2_Well12M
1
iGEM 2019 Distribution Plate 2 Well 12M
iGEM_2019_Plate2_Well23E
1
iGEM 2019 Distribution Plate 2 Well 23E
iGEM_2019_Plate2_Well2H
1
iGEM 2019 Distribution Plate 2 Well 2H
iGEM_2019_Plate2_Well9P
1
iGEM 2019 Distribution Plate 2 Well 9P
iGEM_2019_Plate2_Well16J
1
iGEM 2019 Distribution Plate 2 Well 16J
iGEM_2019_Plate2_Well14L
1
iGEM 2019 Distribution Plate 2 Well 14L
iGEM_2019_Plate2_Well4L
1
iGEM 2019 Distribution Plate 2 Well 4L
iGEM_2019_Plate2_Well4E
1
iGEM 2019 Distribution Plate 2 Well 4E
iGEM_2019_Plate2_Well5M
1
iGEM 2019 Distribution Plate 2 Well 5M
iGEM_2019_Plate2_Well23K
1
iGEM 2019 Distribution Plate 2 Well 23K
iGEM_2019_Plate2_Well3A
1
iGEM 2019 Distribution Plate 2 Well 3A
iGEM_2019_Plate2_Well4C
1
iGEM 2019 Distribution Plate 2 Well 4C
iGEM_2019_Plate2_Well15P
1
iGEM 2019 Distribution Plate 2 Well 15P
iGEM_2019_Plate2_Well14D
1
iGEM 2019 Distribution Plate 2 Well 14D
iGEM_2019_Plate2_Well17E
1
iGEM 2019 Distribution Plate 2 Well 17E
iGEM_2019_Plate2_Well6G
1
iGEM 2019 Distribution Plate 2 Well 6G
iGEM_2019_Plate2_Well24B
1
iGEM 2019 Distribution Plate 2 Well 24B
iGEM_2019_Plate2_Well9F
1
iGEM 2019 Distribution Plate 2 Well 9F
iGEM_2019_Plate2_Well17A
1
iGEM 2019 Distribution Plate 2 Well 17A
iGEM_2019_Plate2_Well5O
1
iGEM 2019 Distribution Plate 2 Well 5O
iGEM_2019_Plate2_Well5H
1
iGEM 2019 Distribution Plate 2 Well 5H
iGEM_2019_Plate2_Well24J
1
iGEM 2019 Distribution Plate 2 Well 24J
iGEM_2019_Plate2_Well3N
1
iGEM 2019 Distribution Plate 2 Well 3N
iGEM_2019_Plate2_Well9K
1
iGEM 2019 Distribution Plate 2 Well 9K
iGEM_2019_Plate2_Well21G
1
iGEM 2019 Distribution Plate 2 Well 21G
iGEM_2019_Plate2_Well13K
1
iGEM 2019 Distribution Plate 2 Well 13K
iGEM_2019_Plate2_Well24O
1
iGEM 2019 Distribution Plate 2 Well 24O
iGEM_2019_Plate2_Well21D
1
iGEM 2019 Distribution Plate 2 Well 21D
iGEM_2019_Plate2_Well11C
1
iGEM 2019 Distribution Plate 2 Well 11C
iGEM_2019_Plate2_Well7B
1
iGEM 2019 Distribution Plate 2 Well 7B
iGEM_2019_Plate2_Well21P
1
iGEM 2019 Distribution Plate 2 Well 21P
iGEM_2019_Plate2_Well4A
1
iGEM 2019 Distribution Plate 2 Well 4A
iGEM_2019_Plate2_Well15H
1
iGEM 2019 Distribution Plate 2 Well 15H
iGEM_2019_Plate2_Well16I
1
iGEM 2019 Distribution Plate 2 Well 16I
iGEM_2019_Plate2_Well1F
1
iGEM 2019 Distribution Plate 2 Well 1F
iGEM_2019_Plate2_Well13P
1
iGEM 2019 Distribution Plate 2 Well 13P
iGEM_2019_Plate2_Well6A
1
iGEM 2019 Distribution Plate 2 Well 6A
iGEM_2019_Plate2_Well2A
1
iGEM 2019 Distribution Plate 2 Well 2A
iGEM_2019_Plate2_Well22E
1
iGEM 2019 Distribution Plate 2 Well 22E
iGEM_2019_Plate2_Well10L
1
iGEM 2019 Distribution Plate 2 Well 10L
iGEM_2019_Plate2_Well19D
1
iGEM 2019 Distribution Plate 2 Well 19D
iGEM_2019_Plate2_Well2P
1
iGEM 2019 Distribution Plate 2 Well 2P
iGEM_2019_Plate2_Well7A
1
iGEM 2019 Distribution Plate 2 Well 7A
iGEM_2019_Plate2_Well5P
1
iGEM 2019 Distribution Plate 2 Well 5P
iGEM_2019_Plate2_Well17J
1
iGEM 2019 Distribution Plate 2 Well 17J
iGEM_2019_Plate2_Well7F
1
iGEM 2019 Distribution Plate 2 Well 7F
iGEM_2019_Plate2_Well22B
1
iGEM 2019 Distribution Plate 2 Well 22B
iGEM_2019_Plate2_Well20E
1
iGEM 2019 Distribution Plate 2 Well 20E
iGEM_2019_Plate2_Well22C
1
iGEM 2019 Distribution Plate 2 Well 22C
iGEM_2019_Plate2_Well14E
1
iGEM 2019 Distribution Plate 2 Well 14E
iGEM_2019_Plate2_Well21K
1
iGEM 2019 Distribution Plate 2 Well 21K
iGEM_2019_Plate2_Well9I
1
iGEM 2019 Distribution Plate 2 Well 9I
iGEM_2019_Plate2_Well4B
1
iGEM 2019 Distribution Plate 2 Well 4B
iGEM_2019_Plate2_Well1C
1
iGEM 2019 Distribution Plate 2 Well 1C
iGEM_2019_Plate2_Well19C
1
iGEM 2019 Distribution Plate 2 Well 19C
iGEM_2019_Plate2_Well2O
1
iGEM 2019 Distribution Plate 2 Well 2O
iGEM_2019_Plate2_Well8N
1
iGEM 2019 Distribution Plate 2 Well 8N
iGEM_2019_Plate2_Well23B
1
iGEM 2019 Distribution Plate 2 Well 23B
iGEM_2019_Plate2_Well4I
1
iGEM 2019 Distribution Plate 2 Well 4I
iGEM_2019_Plate2_Well14P
1
iGEM 2019 Distribution Plate 2 Well 14P
iGEM_2019_Plate2_Well18N
1
iGEM 2019 Distribution Plate 2 Well 18N
iGEM_2019_Plate2_Well11P
1
iGEM 2019 Distribution Plate 2 Well 11P
iGEM_2019_Plate2_Well6J
1
iGEM 2019 Distribution Plate 2 Well 6J
iGEM_2019_Plate2_Well18L
1
iGEM 2019 Distribution Plate 2 Well 18L
iGEM_2019_Plate2_Well11G
1
iGEM 2019 Distribution Plate 2 Well 11G
iGEM_2019_Plate2_Well11F
1
iGEM 2019 Distribution Plate 2 Well 11F
iGEM_2019_Plate2_Well13O
1
iGEM 2019 Distribution Plate 2 Well 13O
iGEM_2019_Plate2_Well23F
1
iGEM 2019 Distribution Plate 2 Well 23F
iGEM_2019_Plate2_Well3C
1
iGEM 2019 Distribution Plate 2 Well 3C
iGEM_2019_Plate2_Well13G
1
iGEM 2019 Distribution Plate 2 Well 13G
iGEM_2019_Plate2_Well13D
1
iGEM 2019 Distribution Plate 2 Well 13D
iGEM_2019_Plate2_Well17H
1
iGEM 2019 Distribution Plate 2 Well 17H
iGEM_2019_Plate2_Well17N
1
iGEM 2019 Distribution Plate 2 Well 17N
iGEM_2019_Plate2_Well9D
1
iGEM 2019 Distribution Plate 2 Well 9D
iGEM_2019_Plate2_Well22O
1
iGEM 2019 Distribution Plate 2 Well 22O
iGEM_2019_Plate2_Well6F
1
iGEM 2019 Distribution Plate 2 Well 6F
iGEM_2019_Plate2_Well8M
1
iGEM 2019 Distribution Plate 2 Well 8M
iGEM_2019_Plate2_Well7K
1
iGEM 2019 Distribution Plate 2 Well 7K
iGEM_2019_Plate2_Well17K
1
iGEM 2019 Distribution Plate 2 Well 17K
iGEM_2019_Plate2_Well18K
1
iGEM 2019 Distribution Plate 2 Well 18K
iGEM_2019_Plate2_Well14G
1
iGEM 2019 Distribution Plate 2 Well 14G
iGEM_2019_Plate2_Well5L
1
iGEM 2019 Distribution Plate 2 Well 5L
iGEM_2019_Plate2_Well13C
1
iGEM 2019 Distribution Plate 2 Well 13C
iGEM_2019_Plate2_Well15E
1
iGEM 2019 Distribution Plate 2 Well 15E
iGEM_2019_Plate2_Well20B
1
iGEM 2019 Distribution Plate 2 Well 20B
iGEM_2019_Plate2_Well2E
1
iGEM 2019 Distribution Plate 2 Well 2E
iGEM_2019_Plate2_Well2F
1
iGEM 2019 Distribution Plate 2 Well 2F
iGEM_2019_Plate2_Well12B
1
iGEM 2019 Distribution Plate 2 Well 12B
iGEM_2019_Plate2_Well5K
1
iGEM 2019 Distribution Plate 2 Well 5K
iGEM_2019_Plate2_Well18I
1
iGEM 2019 Distribution Plate 2 Well 18I
iGEM_2019_Plate2_Well9B
1
iGEM 2019 Distribution Plate 2 Well 9B
iGEM_2019_Plate2_Well23A
1
iGEM 2019 Distribution Plate 2 Well 23A
iGEM_2019_Plate2_Well10E
1
iGEM 2019 Distribution Plate 2 Well 10E
iGEM_2019_Plate2_Well18G
1
iGEM 2019 Distribution Plate 2 Well 18G
iGEM_2019_Plate2_Well12A
1
iGEM 2019 Distribution Plate 2 Well 12A
iGEM_2019_Plate2_Well16K
1
iGEM 2019 Distribution Plate 2 Well 16K
iGEM_2019_Plate2_Well20J
1
iGEM 2019 Distribution Plate 2 Well 20J
iGEM_2019_Plate2_Well18E
1
iGEM 2019 Distribution Plate 2 Well 18E
iGEM_2019_Plate2_Well2D
1
iGEM 2019 Distribution Plate 2 Well 2D
iGEM_2019_Plate2_Well1O
1
iGEM 2019 Distribution Plate 2 Well 1O
iGEM_2019_Plate2_Well9J
1
iGEM 2019 Distribution Plate 2 Well 9J
iGEM_2019_Plate2_Well7H
1
iGEM 2019 Distribution Plate 2 Well 7H
iGEM_2019_Plate2_Well23I
1
iGEM 2019 Distribution Plate 2 Well 23I
iGEM_2019_Plate2_Well18A
1
iGEM 2019 Distribution Plate 2 Well 18A
iGEM_2019_Plate2_Well12L
1
iGEM 2019 Distribution Plate 2 Well 12L
iGEM_2019_Plate2_Well24P
1
iGEM 2019 Distribution Plate 2 Well 24P
iGEM_2019_Plate2_Well17P
1
iGEM 2019 Distribution Plate 2 Well 17P
iGEM_2019_Plate2_Well9C
1
iGEM 2019 Distribution Plate 2 Well 9C
iGEM_2019_Plate2_Well20O
1
iGEM 2019 Distribution Plate 2 Well 20O
iGEM_2019_Plate2_Well9A
1
iGEM 2019 Distribution Plate 2 Well 9A
iGEM_2019_Plate2_Well8K
1
iGEM 2019 Distribution Plate 2 Well 8K
iGEM_2019_Plate2_Well11K
1
iGEM 2019 Distribution Plate 2 Well 11K
iGEM_2019_Plate2_Well1N
1
iGEM 2019 Distribution Plate 2 Well 1N
iGEM_2019_Plate2_Well14C
1
iGEM 2019 Distribution Plate 2 Well 14C
iGEM_2019_Plate2_Well8F
1
iGEM 2019 Distribution Plate 2 Well 8F
iGEM_2019_Plate2_Well8L
1
iGEM 2019 Distribution Plate 2 Well 8L
iGEM_2019_Plate2_Well1P
1
iGEM 2019 Distribution Plate 2 Well 1P
iGEM_2019_Plate2_Well7P
1
iGEM 2019 Distribution Plate 2 Well 7P
iGEM_2019_Plate2_Well8P
1
iGEM 2019 Distribution Plate 2 Well 8P
iGEM_2019_Plate2_Well7M
1
iGEM 2019 Distribution Plate 2 Well 7M
iGEM_2019_Plate2_Well13M
1
iGEM 2019 Distribution Plate 2 Well 13M
iGEM_2019_Plate2_Well16E
1
iGEM 2019 Distribution Plate 2 Well 16E
iGEM_2019_Plate2_Well19O
1
iGEM 2019 Distribution Plate 2 Well 19O
iGEM_2019_Plate2_Well16D
1
iGEM 2019 Distribution Plate 2 Well 16D
iGEM_2019_Plate2_Well20N
1
iGEM 2019 Distribution Plate 2 Well 20N
iGEM_2019_Plate2_Well11J
1
iGEM 2019 Distribution Plate 2 Well 11J
iGEM_2019_Plate2_Well10M
1
iGEM 2019 Distribution Plate 2 Well 10M
iGEM_2019_Plate2_Well24D
1
iGEM 2019 Distribution Plate 2 Well 24D
iGEM_2019_Plate2_Well21I
1
iGEM 2019 Distribution Plate 2 Well 21I
iGEM_2019_Plate2_Well4D
1
iGEM 2019 Distribution Plate 2 Well 4D
iGEM_2019_Plate2_Well16N
1
iGEM 2019 Distribution Plate 2 Well 16N
iGEM_2019_Plate2_Well8G
1
iGEM 2019 Distribution Plate 2 Well 8G
iGEM_2019_Plate2_Well10B
1
iGEM 2019 Distribution Plate 2 Well 10B
iGEM_2019_Plate2_Well19J
1
iGEM 2019 Distribution Plate 2 Well 19J
iGEM_2019_Plate2_Well17D
1
iGEM 2019 Distribution Plate 2 Well 17D
iGEM_2019_Plate2_Well1A
1
iGEM 2019 Distribution Plate 2 Well 1A
iGEM_2019_Plate2_Well19M
1
iGEM 2019 Distribution Plate 2 Well 19M
iGEM_2019_Plate2_Well3J
1
iGEM 2019 Distribution Plate 2 Well 3J
iGEM_2019_Plate2_Well21C
1
iGEM 2019 Distribution Plate 2 Well 21C
iGEM_2019_Plate2_Well2I
1
iGEM 2019 Distribution Plate 2 Well 2I
iGEM_2019_Plate2_Well3L
1
iGEM 2019 Distribution Plate 2 Well 3L
iGEM_2019_Plate2_Well22F
1
iGEM 2019 Distribution Plate 2 Well 22F
iGEM_2019_Plate2_Well14M
1
iGEM 2019 Distribution Plate 2 Well 14M
iGEM_2019_Plate2_Well8E
1
iGEM 2019 Distribution Plate 2 Well 8E
iGEM_2019_Plate2_Well19H
1
iGEM 2019 Distribution Plate 2 Well 19H
iGEM_2019_Plate2_Well7N
1
iGEM 2019 Distribution Plate 2 Well 7N
iGEM_2019_Plate2_Well1H
1
iGEM 2019 Distribution Plate 2 Well 1H
iGEM_2019_Plate2_Well19E
1
iGEM 2019 Distribution Plate 2 Well 19E
iGEM_2019_Plate2_Well18M
1
iGEM 2019 Distribution Plate 2 Well 18M
iGEM_2019_Plate2_Well15M
1
iGEM 2019 Distribution Plate 2 Well 15M
iGEM_2019_Plate2_Well8A
1
iGEM 2019 Distribution Plate 2 Well 8A
iGEM_2019_Plate2_Well15L
1
iGEM 2019 Distribution Plate 2 Well 15L
iGEM_2019_Plate2_Well10H
1
iGEM 2019 Distribution Plate 2 Well 10H
iGEM_2019_Plate2_Well10O
1
iGEM 2019 Distribution Plate 2 Well 10O
iGEM_2019_Plate2_Well10C
1
iGEM 2019 Distribution Plate 2 Well 10C
iGEM_2019_Plate2_Well22G
1
iGEM 2019 Distribution Plate 2 Well 22G
iGEM_2019_Plate2_Well6H
1
iGEM 2019 Distribution Plate 2 Well 6H
iGEM_2019_Plate2_Well13N
1
iGEM 2019 Distribution Plate 2 Well 13N
iGEM_2019_Plate2_Well21J
1
iGEM 2019 Distribution Plate 2 Well 21J
iGEM_2019_Plate2_Well3G
1
iGEM 2019 Distribution Plate 2 Well 3G
iGEM_2019_Plate2_Well21F
1
iGEM 2019 Distribution Plate 2 Well 21F
iGEM_2019_Plate2_Well22P
1
iGEM 2019 Distribution Plate 2 Well 22P
iGEM_2019_Plate2_Well17I
1
iGEM 2019 Distribution Plate 2 Well 17I
iGEM_2019_Plate2_Well13H
1
iGEM 2019 Distribution Plate 2 Well 13H
iGEM_2019_Plate2_Well21O
1
iGEM 2019 Distribution Plate 2 Well 21O
iGEM_2019_Plate2_Well19B
1
iGEM 2019 Distribution Plate 2 Well 19B
iGEM_2019_Plate2_Well17L
1
iGEM 2019 Distribution Plate 2 Well 17L
iGEM_2019_Plate2_Well16H
1
iGEM 2019 Distribution Plate 2 Well 16H
iGEM_2019_Plate2_Well13J
1
iGEM 2019 Distribution Plate 2 Well 13J
iGEM_2019_Plate2_Well19A
1
iGEM 2019 Distribution Plate 2 Well 19A
iGEM_2019_Plate2_Well11N
1
iGEM 2019 Distribution Plate 2 Well 11N
iGEM_2019_Plate2_Well7L
1
iGEM 2019 Distribution Plate 2 Well 7L
iGEM_2019_Plate2_Well7C
1
iGEM 2019 Distribution Plate 2 Well 7C
iGEM_2019_Plate2
1
iGEM 2019 Distribution Plate 2
iGEM_2019_Plate2_Well15B
1
iGEM 2019 Distribution Plate 2 Well 15B
iGEM_2019_Plate2_Well12C
1
iGEM 2019 Distribution Plate 2 Well 12C
iGEM_2019_Plate2_Well22H
1
iGEM 2019 Distribution Plate 2 Well 22H
iGEM_2019_Plate2_Well3M
1
iGEM 2019 Distribution Plate 2 Well 3M
iGEM_2019_Plate2_Well1B
1
iGEM 2019 Distribution Plate 2 Well 1B
iGEM_2019_Plate2_Well9M
1
iGEM 2019 Distribution Plate 2 Well 9M
iGEM_2019_Plate2_Well12D
1
iGEM 2019 Distribution Plate 2 Well 12D
iGEM_2019_Plate2_Well4F
1
iGEM 2019 Distribution Plate 2 Well 4F
iGEM_2019_Plate2_Well20C
1
iGEM 2019 Distribution Plate 2 Well 20C
iGEM_2019_Plate2_Well11E
1
iGEM 2019 Distribution Plate 2 Well 11E
iGEM_2019_Plate2_Well21M
1
iGEM 2019 Distribution Plate 2 Well 21M
iGEM_2019_Plate2_Well17F
1
iGEM 2019 Distribution Plate 2 Well 17F
iGEM_2019_Plate2_Well17G
1
iGEM 2019 Distribution Plate 2 Well 17G
iGEM_2019_Plate2_Well19L
1
iGEM 2019 Distribution Plate 2 Well 19L
iGEM_2019_Plate2_Well15K
1
iGEM 2019 Distribution Plate 2 Well 15K
iGEM_2019_Plate2_Well12F
1
iGEM 2019 Distribution Plate 2 Well 12F
iGEM_2019_Plate2_Well2B
1
iGEM 2019 Distribution Plate 2 Well 2B
iGEM_2019_Plate2_Well21N
1
iGEM 2019 Distribution Plate 2 Well 21N
iGEM_2019_Plate2_Well23J
1
iGEM 2019 Distribution Plate 2 Well 23J
iGEM_2019_Plate2_Well3O
1
iGEM 2019 Distribution Plate 2 Well 3O
iGEM_2019_Plate2_Well24L
1
iGEM 2019 Distribution Plate 2 Well 24L
iGEM_2019_Plate2_Well16F
1
iGEM 2019 Distribution Plate 2 Well 16F
iGEM_2019_Plate2_Well9G
1
iGEM 2019 Distribution Plate 2 Well 9G
iGEM_2019_Plate2_Well22K
1
iGEM 2019 Distribution Plate 2 Well 22K
iGEM_2019_Plate2_Well13L
1
iGEM 2019 Distribution Plate 2 Well 13L
iGEM_2019_Plate2_Well18B
1
iGEM 2019 Distribution Plate 2 Well 18B
iGEM_2019_Plate2_Well9L
1
iGEM 2019 Distribution Plate 2 Well 9L
iGEM_2019_Plate2_Well7O
1
iGEM 2019 Distribution Plate 2 Well 7O
iGEM_2019_Plate2_Well3B
1
iGEM 2019 Distribution Plate 2 Well 3B
iGEM_2019_Plate2_Well5F
1
iGEM 2019 Distribution Plate 2 Well 5F
iGEM_2019_Plate2_Well3E
1
iGEM 2019 Distribution Plate 2 Well 3E
iGEM_2019_Plate2_Well21L
1
iGEM 2019 Distribution Plate 2 Well 21L
iGEM_2019_Plate2_Well21A
1
iGEM 2019 Distribution Plate 2 Well 21A
iGEM_2019_Plate2_Well8I
1
iGEM 2019 Distribution Plate 2 Well 8I
iGEM_2019_Plate2_Well1G
1
iGEM 2019 Distribution Plate 2 Well 1G
iGEM_2019_Plate2_Well2K
1
iGEM 2019 Distribution Plate 2 Well 2K
iGEM_2019_Plate2_Well2C
1
iGEM 2019 Distribution Plate 2 Well 2C
iGEM_2019_Plate2_Well10A
1
iGEM 2019 Distribution Plate 2 Well 10A
iGEM_2019_Plate2_Well18J
1
iGEM 2019 Distribution Plate 2 Well 18J
iGEM_2019_Plate2_Well18O
1
iGEM 2019 Distribution Plate 2 Well 18O
iGEM_2019_Plate2_Well8C
1
iGEM 2019 Distribution Plate 2 Well 8C
iGEM_2019_Plate2_Well14A
1
iGEM 2019 Distribution Plate 2 Well 14A
iGEM_2019_Plate2_Well16L
1
iGEM 2019 Distribution Plate 2 Well 16L
iGEM_2019_Plate2_Well7D
1
iGEM 2019 Distribution Plate 2 Well 7D
iGEM_2019_Plate2_Well20P
1
iGEM 2019 Distribution Plate 2 Well 20P
iGEM_2019_Plate2_Well4H
1
iGEM 2019 Distribution Plate 2 Well 4H
iGEM_2019_Plate2_Well10K
1
iGEM 2019 Distribution Plate 2 Well 10K
iGEM_2019_Plate2_Well7E
1
iGEM 2019 Distribution Plate 2 Well 7E
BBa_I732812
1
BBa_I732812
ARL2A0301 (RBS+, TERM-)
Zhan Jian
2007-10-13T11:00:00Z
2015-08-31T04:07:59Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1946199
1
BBa_I732112
component1946198
1
BBa_B0034
annotation1946199
1
BBa_I732112
range1946199
1
19
1104
annotation1946198
1
BBa_B0034
range1946198
1
1
12
BBa_K773003
1
BBa_K773003
mCherry-LVA
Katie Knister
2012-09-30T11:00:00Z
2015-05-08T01:13:15Z
false
false
_1025_
0
11646
9
Released HQ 2013
In stock
false
This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
We modified the AAV sequence to LVA with PCR.
This part was modified from a plasmid provided by the Murray lab at Caltech.
false
annotation2209512
1
Stop
range2209512
1
760
762
annotation2209510
1
Start
range2209510
1
19
21
annotation2209511
1
ssrA degradation tag: LVA
range2209511
1
730
759
annotation2209508
1
B0034
range2209508
1
1
12
annotation2209513
1
Stop
range2209513
1
763
765
annotation2209509
1
mCherry-LVA
range2209509
1
19
765
BBa_I718012
1
BBa_I718012
ptet-lox71-ter
David Bikard
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
false
false
_141_
0
1481
9
Released HQ 2013
In stock
false
Upstream construct of the Synthetics Multicellular Bacterium
none
none
false
component1956337
1
BBa_R0040
component1956341
1
BBa_I718017
component1956342
1
BBa_B0010
component1956344
1
BBa_B0012
annotation1956337
1
BBa_R0040
range1956337
1
1
54
annotation1956342
1
BBa_B0010
range1956342
1
105
184
annotation1956344
1
BBa_B0012
range1956344
1
193
233
annotation1956341
1
BBa_I718017
range1956341
1
63
96
BBa_K812010
1
GFP-AID
GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)
Cyrille Pauthenier
2012-09-17T11:00:00Z
2015-05-08T01:13:27Z
false
true
_1069_
0
8998
9
Released HQ 2013
In stock
false
This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell.
This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and
Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
This part has been biobricked using PCR method and cloned in pSB1C3
This part have been extracted by PCR from the plasmid pNHK60 obtained from the riken registry.
false
annotation2184369
1
Kozac
range2184369
1
1
6
annotation2184373
1
NLS1
range2184373
1
1459
1479
annotation2184374
1
NLS2
range2184374
1
1480
1500
annotation2184371
1
unfolded linker
range2184371
1
724
756
annotation2184370
1
GFP
range2184370
1
7
723
annotation2184372
1
AID degradation tag
range2184372
1
757
1443
BBa_K861031
1
BBa_K861031
FadJ, gene for fatty acid degradation from <i>E.coli str. K12</i>
Kuanwei Sheng
2012-09-05T11:00:00Z
2015-05-08T01:13:35Z
false
false
_1121_
0
12357
9
Released HQ 2013
In stock
false
FadJ is the anaerobic counterpart of FadB. The protein is shown to have better degradation of medium and short length fatty acid together with FadI.
None
E.coli str.K12 lab mutant DH5α
false
BBa_S0116
1
BBa_S0116
B0031.C0052
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-05-08T01:14:17Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component939974
1
BBa_B0031
component939984
1
BBa_C0052
annotation939984
1
BBa_C0052
range939984
1
21
689
annotation939974
1
BBa_B0031
range939974
1
1
14
BBa_J202010
1
BBa_J202010
PBAD Mutant#11
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -58 relative to the araC transcriptional start site was changed from A to C.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178457
1
Mutation
range2178457
1
54
54
annotation2178459
1
araI1
range2178459
1
40
56
annotation2178458
1
Transcription Start
range2178458
1
112
112
annotation2178460
1
araI2
range2178460
1
61
77
BBa_J06602
1
BBa_J06602
Construction Intermediate: mCherry, bacterial with RBS (B0034.J06504)
ytwang
2005-06-13T11:00:00Z
2015-08-31T04:08:18Z
false
true
_20_
0
340
20
Released HQ 2013
In stock
false
Combines BBa_B0034 RBS (Elowitz 1999) with BBa_J06504 mCherry, bacterial.
This is an intermediate leading to BBa_J06702.
false
component1596326
1
BBa_B0034
component1596334
1
BBa_J06504
annotation1596326
1
BBa_B0034
range1596326
1
1
12
annotation1596334
1
BBa_J06504
range1596334
1
19
732
BBa_R0080
1
AraC
Promoter (AraC regulated)
Sara Neves (Fighting Darwins)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
GenBank: J01641 (www.ncbi.nlm.nih.gov)
true
annotation301456
1
c-amp2
range301456
1
4
29
annotation301457
1
araO1
range301457
1
6
44
annotation308602
1
-10
range308602
1
136
141
annotation308601
1
-35
range308601
1
113
118
annotation301458
1
c-amp1
range301458
1
43
72
annotation301462
1
ara1 and ara2
range301462
1
73
101
BBa_K822002
1
BBa_K822002
Colicin E1 + immunoprotein
Jarle Pahr
2012-09-22T11:00:00Z
2015-05-08T01:13:29Z
false
false
_1080_
0
11627
9
Released HQ 2013
In stock
true
Colicin E1 operon of E. coli expressed under a constitutive promoter (BBa_J23119).
BBa_K150007 was not available from the registry, so its sequence was cloned from K150009 by PCR.
BBa_J23119, BBa_B0034 and K150009/BBa_K150007.
false
annotation2197335
1
Kil protein fragment
range2197335
1
1955
2050
annotation2197334
1
ColE1 immunity protein
range2197334
1
1566
1907
annotation2197333
1
ColE1 protein
range2197333
1
1
1569
BBa_K598014
1
BBa_K598014
pBAD+HH+CdIntron+E0040+b0015
Xiaowei YAN, Tong MU
2011-09-26T11:00:00Z
2015-05-08T01:12:50Z
false
false
_770_
0
8713
9
Released HQ 2013
In stock
false
This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
The intron derives from the genome of Cd
false
annotation2153088
1
RBS
range2153088
1
187
191
annotation2153083
1
group I intron
range2153083
1
186
764
annotation2153086
1
eGFP
range2153086
1
765
1484
annotation2153081
1
pBAD
range2153081
1
1
130
annotation2153082
1
HH
range2153082
1
139
185
annotation2153089
1
RBS
range2153089
1
758
758
annotation2153087
1
BBa_B0015
range2153087
1
1493
1621
BBa_E0022
1
ECFP
enhanced cyan fluorescent protein derived from A. victoria GFP
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
2003-01-31T12:00:00Z
2015-08-31T04:07:25Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. </P>
<P> <P>BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
Modified from <bb_part>BBa_E0021</bb_part>.
true
annotation2150
1
CFP (LVA)
range2150
1
1
762
annotation2155
1
A
range2155
1
69
69
annotation2153
1
SsrA
range2153
1
719
756
annotation2154
1
2
range2154
1
757
762
annotation7040
1
BBa_E0022
range7040
1
1
762
BBa_I732903
1
BBa_I732903
R0011 I732020
Zhan Jian
2007-10-16T11:00:00Z
2015-08-31T04:08:00Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1948581
1
BBa_B0010
component1948583
1
BBa_B0012
component1948576
1
BBa_B0034
component1948580
1
BBa_I732006
component1948570
1
BBa_R0011
annotation1948581
1
BBa_B0010
range1948581
1
324
403
annotation1948570
1
BBa_R0011
range1948570
1
1
54
annotation1948583
1
BBa_B0012
range1948583
1
412
452
annotation1948576
1
BBa_B0034
range1948576
1
64
75
annotation1948580
1
BBa_I732006
range1948580
1
82
315
BBa_K812032
1
BBa_K812032
mCFP with kozak sequence for eukaryotic expression
Chauris Karine
2012-09-19T11:00:00Z
2015-05-08T01:13:27Z
false
true
_1069_
0
12937
9
Released HQ 2013
In stock
false
mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts.
PCR and cloned in pSB1C3 using EcoRI and PstI
PCR amplified with kozak sequence from DNA coming from Alfonso Jaramillo's lab
false
annotation2187736
1
Kozak
range2187736
1
1
5
annotation2187737
1
mCFP
range2187737
1
6
722
BBa_K847060
1
otsA
''Escherichia coli'' osmoregulatory trehalose synthesis A (otsA)
Rashmi Sharma, Vishesh Jain, Kendrick Wang
2012-08-08T11:00:00Z
2015-05-08T01:13:34Z
false
false
_1107_
0
11092
9
Released HQ 2013
In stock
false
tba
tba
tba
false
BBa_I6050
1
BBa_I6050
Promoter O_H Test R1051.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948694
1
BBa_E0030
component948699
1
BBa_B0010
component948692
1
BBa_B0034
component948684
1
BBa_R1051
component948709
1
BBa_B0012
annotation948709
1
BBa_B0012
range948709
1
895
935
annotation948692
1
BBa_B0034
range948692
1
58
69
annotation948694
1
BBa_E0030
range948694
1
76
798
annotation948684
1
BBa_R1051
range948684
1
1
49
annotation948699
1
BBa_B0010
range948699
1
807
886
BBa_J202000
1
BBa_J202000
PBAD mutant#1
Jiyeon Park
2012-07-18T11:00:00Z
2015-08-31T04:08:36Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is mutant of PBAD (BBa_I13453).
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part lacks araO2 site which is essential for araC repressor function, araC acts as an activator in the presence of L-arabinose.
This mutant was created by random mutagenesis.
false
annotation2177772
1
araC transcription start
range2177772
1
112
112
annotation2177770
1
araI1
range2177770
1
40
56
annotation2177773
1
Mutation
range2177773
1
57
57
annotation2177771
1
araI2
range2177771
1
61
77
BBa_E0422
1
BBa_E0422
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-08-31T04:07:26Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
true
component942815
1
BBa_E0022
component942803
1
BBa_B0034
component942823
1
BBa_B0010
component942833
1
BBa_B0012
annotation942815
1
BBa_E0022
range942815
1
19
780
annotation942803
1
BBa_B0034
range942803
1
1
12
annotation942833
1
BBa_B0012
range942833
1
877
917
annotation942823
1
BBa_B0010
range942823
1
789
868
BBa_J202008
1
BBa_J202008
PBAD Mutant#9
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -60 relative to the araC transcriptional start site was changed from C to T.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178449
1
araI1
range2178449
1
40
56
annotation2178448
1
Mutation
range2178448
1
52
52
annotation2178450
1
araI2
range2178450
1
61
77
annotation2178451
1
Transcription Start
range2178451
1
112
112
BBa_K775009
1
BBa_K775009
Niacin Chimeric GPCR with Fus1-EGFP reporter (2)
Sietske Grijseels
2012-09-23T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1027_
0
12035
9
Released HQ 2013
In stock
false
-
-
-
false
component2195587
1
BBa_K775000
component2195575
1
BBa_K775004
annotation2195575
1
BBa_K775004
range2195575
1
1
1166
annotation2195587
1
BBa_K775000
range2195587
1
1175
2588
BBa_J202003
1
BBa_J202003
PBAD Mutant#4
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -63(T to C), -42 (C to A), and +9 (T to A) relative to the araC transcriptional start site were changed.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by random mutagenesis.
false
annotation2178426
1
Transcription Start
range2178426
1
112
112
annotation2178427
1
araI1
range2178427
1
40
56
annotation2178431
1
Mutation 3
range2178431
1
120
120
annotation2178429
1
Mutation 1
range2178429
1
49
49
annotation2178430
1
Mutation 2
range2178430
1
70
70
annotation2178428
1
araI2
range2178428
1
61
77
BBa_J01142
1
BBa_J01142
[pLacI pL][key3]
Golden Bear (Berkeley iGEM 2005)
2006-04-13T11:00:00Z
2015-08-31T04:08:13Z
false
false
_13_1_
0
612
13
Released HQ 2013
In stock
false
-- No description --
false
component1843668
1
BBa_R0011
component1843674
1
BBa_J01086
annotation1843674
1
BBa_J01086
range1843674
1
64
157
annotation1843668
1
BBa_R0011
range1843668
1
1
54
BBa_K808000
1
Ara
araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
Valentina Herbring, Sebastian Palluk, Andreas Schmidt
2012-08-10T11:00:00Z
2015-05-08T01:13:25Z
false
false
_1065_
0
11792
9
Released HQ 2013
In stock
true
This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
non
Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30
Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
false
annotation2200758
1
AraO
range2200758
1
909
924
annotation2200759
1
AraO1
range2200759
1
1066
1077
annotation2200760
1
AraPromoter
range2200760
1
1153
1181
annotation2200757
1
AraC
range2200757
1
1
879
BBa_I715022
1
RFP1
Amino Portion of RFP
Andrew Martens
2007-06-24T11:00:00Z
2016-01-25T04:43:58Z
false
false
_120_
4206
201
61
Released HQ 2013
In stock
false
af
afd
adf
true
annotation1935381
1
Coding sequence
range1935381
1
1
462
annotation1935380
1
start
range1935380
1
1
3
BBa_J202007
1
BBa_J202007
PBAD Mutnat#8
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -62 relative to the araC transcriptional start site was changed from A to G.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178447
1
Mutation
range2178447
1
50
50
annotation2178445
1
araI1
range2178445
1
40
56
annotation2178446
1
araI2
range2178446
1
61
77
annotation2178444
1
Transcription Start
range2178444
1
112
112
BBa_J202005
1
BBa_J202005
PBAD Mutant#6
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -67 relative to the araC transcriptional start site was changed from T to A.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178437
1
araI1
range2178437
1
40
56
annotation2178439
1
Mutation
range2178439
1
45
45
annotation2178436
1
Transcription Start
range2178436
1
112
112
annotation2178438
1
araI2
range2178438
1
61
77
BBa_K909005
1
BBa_K909005
cI protein with EYFP as a reporter
Deborah Huber
2012-09-22T11:00:00Z
2015-05-08T01:13:44Z
false
false
_1174_
0
14088
9
Released HQ 2013
In stock
false
sdkuhdshkudak
dsasad
sddas
false
component2264560
1
BBa_B0034
component2264564
1
BBa_C0051
component2264574
1
BBa_E0430
annotation2264574
1
BBa_E0430
range2264574
1
802
1679
annotation2264560
1
BBa_B0034
range2264560
1
1
12
annotation2264564
1
BBa_C0051
range2264564
1
19
793
BBa_K737007
1
BBa_K737007
Constitutive GVPC Generator
Li Kang
2012-09-17T11:00:00Z
2015-05-08T01:13:07Z
false
false
_986_
0
14291
9
Released HQ 2013
In stock
false
"It contains a constitutive promoter, GvpC protein , GFP protein and a terminator.
This part can be used as a control of (J23106+P0440+R0040+B0034+GVPC+E0840). It can express GvpC protein and GFP constantly. When it transformed with (J23106+P0412+R0011+B0034+GVPA+E0840), the bacteria may can float with GFP!"
This part can be used as a control of (J23106+P0440+R0040+B0034+GVPC+E0840). It can express GvpC protein and GFP constantly. When it transformed with (J23106+P0412+R0011+B0034+GVPA+E0840), the bacteria may can float with GFP.
Yes.
false
component2184892
1
BBa_B0034
component2184904
1
BBa_E0840
component2184893
1
BBa_K737017
component2184890
1
BBa_J23106
annotation2184892
1
BBa_B0034
range2184892
1
44
55
annotation2184890
1
BBa_J23106
range2184890
1
1
35
annotation2184893
1
BBa_K737017
range2184893
1
62
565
annotation2184904
1
BBa_E0840
range2184904
1
574
1451
BBa_K808034
1
BBa_K808034
araC-Pbad regulated terephtalate periplasmatic binding proteine of the tripartite transporter family
Valentina Herbring, Sebastian Palluk, Andreas Schmidt
2012-09-05T11:00:00Z
2015-05-08T01:13:26Z
false
false
_1065_
0
12529
9
Released HQ 2013
In stock
false
-
-
-
false
component2201271
1
BBa_K808000
component2201273
1
BBa_K808001
component2201272
1
BBa_J61101
annotation2201272
1
BBa_J61101
range2201272
1
1218
1229
annotation2201273
1
BBa_K808001
range2201273
1
1236
2204
annotation2201271
1
BBa_K808000
range2201271
1
1
1209
BBa_J44001
1
BBa_J44001
Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
Todd Eckdahl
2006-08-01T11:00:00Z
2015-08-31T04:08:48Z
false
false
_61_71_
0
606
61
Released HQ 2013
In stock
true
Reverse version of RBS BBa_B0030
Repeats in oligos caused unusual products during cloning
Cloned from synthetic oligos
true
annotation1893199
1
Reverse RBS
range1893199
1
1
15
BBa_I13523
1
BBa_I13523
Screening plasmid v2
jkm
2005-07-18T11:00:00Z
2015-08-31T04:07:35Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
Intermediate in screening plasmid v2 construction.
false
component1597485
1
BBa_B0010
component1597478
1
BBa_B0034
component1597473
1
BBa_I13455
component1597480
1
BBa_E0040
component1597495
1
BBa_B0012
annotation1597495
1
BBa_B0012
range1597495
1
921
961
annotation1597473
1
BBa_I13455
range1597473
1
1
78
annotation1597478
1
BBa_B0034
range1597478
1
87
98
annotation1597485
1
BBa_B0010
range1597485
1
833
912
annotation1597480
1
BBa_E0040
range1597480
1
105
824
BBa_I732805
1
BBa_I732805
ARL2A0101 (RBS+, TERM-)
Zhan Jian
2007-10-13T11:00:00Z
2015-08-31T04:07:59Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1946178
1
BBa_I732105
component1946177
1
BBa_B0034
annotation1946177
1
BBa_B0034
range1946177
1
1
12
annotation1946178
1
BBa_I732105
range1946178
1
19
1104
BBa_I763015
1
RBS-LacY
RBS-LacY
Alice Pasini
2007-08-19T11:00:00Z
2015-08-31T04:08:08Z
false
false
_111_
0
2088
9
Released HQ 2013
In stock
false
PLac activation induces the expression of the Lactose permase (Lac Y) gene, LacI repressor protein with GFP (+LVA tag) as a reporter.
---
Igem 2007 plates
false
component1943313
1
BBa_J22101
component1943312
1
BBa_B0034
annotation1943312
1
BBa_B0034
range1943312
1
1
12
annotation1943313
1
BBa_J22101
range1943313
1
19
1306
BBa_C0053
1
c2 P22
c2 repressor from Salmonella phage P22 (+LVA)
Maia Mahoney
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.</p>
References (unparsed) here: <p>Vander Byl,C. and Kropinski,A.M. <em>Sequence of the genome of Salmonella bacteriophage P22</em><br> J. Bacteriol. 182 (22), 6472-6481 (2000) <P> References (unparsed) here: <p>Vander Byl,C. and Kropinski,A.M. <em>Sequence of the genome of Salmonella bacteriophage P22</em><br> J. Bacteriol. 182 (22), 6472-6481 (2000) <P><P>
Bacteriophage P22
true
annotation1751
1
stop
range1751
1
682
687
annotation2213993
1
Help:Barcodes
range2213993
1
688
712
annotation1747
1
cII p22
range1747
1
1
648
annotation1750
1
LVA
range1750
1
649
681
annotation7036
1
BBa_C0053
range7036
1
1
687
BBa_I718009
1
BBa_I718009
pTet-lox71-rbs-gfp-ter
David Bikard
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
false
false
_141_
0
1481
9
Released HQ 2013
In stock
false
Upstream construct of the synthetic multicellular bacteria
none
none
false
component1956135
1
BBa_B0012
component1956129
1
BBa_B0030
component1956132
1
BBa_E0040
component1956123
1
BBa_R0040
component1956127
1
BBa_J37028
component1956133
1
BBa_B0010
annotation1956135
1
BBa_B0012
range1956135
1
942
982
annotation1956132
1
BBa_E0040
range1956132
1
126
845
annotation1956129
1
BBa_B0030
range1956129
1
105
119
annotation1956123
1
BBa_R0040
range1956123
1
1
54
annotation1956127
1
BBa_J37028
range1956127
1
63
96
annotation1956133
1
BBa_B0010
range1956133
1
854
933
BBa_I742109
1
rbs+COMT
COMT gene with ribosome binding site
sarah hollingshead
2007-10-18T11:00:00Z
2015-08-31T04:08:02Z
false
false
_123_
0
1964
9
Released HQ 2013
In stock
true
COMT (caffeic acid-O-methyl transferase) with ribosome binding site. Enzyme methylates -OH on C4 of the caffeic acid aromatic ring to produce ferulic acid.
COMT is the third gene in the vanillin biosynthesis pathway,
Used COMT cDNA as genomic DNA contains introns
Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006)
Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991)
Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
true
component1949771
1
BBa_I742107
component1949770
1
BBa_J15001
annotation1949771
1
BBa_I742107
range1949771
1
17
1117
annotation1949770
1
BBa_J15001
range1949770
1
1
10
BBa_I732815
1
BBa_I732815
ARL4A0604 (RBS+, TERM-)
Zhan Jian
2007-10-13T11:00:00Z
2015-08-31T04:07:59Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1946208
1
BBa_I732115
component1946207
1
BBa_B0034
annotation1946207
1
BBa_B0034
range1946207
1
1
12
annotation1946208
1
BBa_I732115
range1946208
1
19
1104
BBa_R0083
1
OmpR
Promoter (OmpR, positive)
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown.
The a at base 41 is mutated to t to avoid XbaI
site. Speculation that this was a previous assembly site.
NC_000193 E. coli K12
true
annotation301223
1
linker
range301223
1
37
44
annotation301224
1
-35
range301224
1
45
50
annotation306881
1
A
range306881
1
41
41
annotation301226
1
-10
range301226
1
68
73
annotation301221
1
C1 OmpR
range301221
1
13
30
BBa_J63008
1
SV40 NLS
SV40 nuclear localization sequence from SV40; yeast codon optimized
Caroline Ajo-Franklin
2006-10-10T11:00:00Z
2015-08-31T01:56:26Z
false
true
_97_
0
545
97
Released HQ 2013
In stock
false
yeast codon-optimized nuclear localization sequence from simian virus 40
as described in literature
direct synthesis from published sequence
true
BBa_K876015
1
BBa_K876015
pTet-LasR-DT
Timothy Cogan
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
true
_1138_
0
13836
9
Released HQ 2013
In stock
false
Constitutive promoter for LasR which can be repressed by TetR
Biobrick placement
Created from biobricks
false
component2251235
1
BBa_C0079
component2251226
1
BBa_R0040
component2251242
1
BBa_B0015
annotation2251235
1
BBa_C0079
range2251235
1
61
841
annotation2251226
1
BBa_R0040
range2251226
1
1
54
annotation2251242
1
BBa_B0015
range2251242
1
850
978
BBa_M30011
1
BBa_M30011
OmpR controlled mRFP
natalie kuldell
2005-12-14T12:00:00Z
2015-09-17T06:41:23Z
false
true
_45_1_
25546
314
1
Released HQ 2013
In stock
false
Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of mRFP (I13507). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. An alternative clone is M30012. Cells bearing this construct are pale pink.
false
component1768968
1
BBa_R0082
component1768973
1
BBa_B0034
component1768986
1
BBa_B0010
component1768996
1
BBa_B0012
component1768980
1
BBa_E1010
annotation1768996
1
BBa_B0012
range1768996
1
937
977
annotation1768986
1
BBa_B0010
range1768986
1
849
928
annotation1768968
1
BBa_R0082
range1768968
1
1
108
annotation1768973
1
BBa_B0034
range1768973
1
117
128
annotation1768980
1
BBa_E1010
range1768980
1
135
815
BBa_K952003
1
BBa_K952003
B0034-YF1-B0034-FixJ-PfixK2-amilGFP
Saimon Sharif
2012-09-30T11:00:00Z
2015-05-08T01:13:49Z
false
false
_1220_
0
13332
9
Released HQ 2013
In stock
false
This part is a composite of BBa_K592016, BBa_K592006, and BBa_K592010 in that order. When the cells are exposed to blue-light, they will express amilGFP, a yellow chromoprotein. This protein causes the cells to have a strong yellow color.
N/A
BBa_K592016, BBa_K592006, and BBa_K592010 are available in the parts registry. They were obtained directly from the Uppsala-Sweden 2011 team.
false
component2205329
1
BBa_K592006
component2205331
1
BBa_K592010
component2205327
1
BBa_K592016
annotation2205331
1
BBa_K592010
range2205331
1
2061
2759
annotation2205327
1
BBa_K592016
range2205327
1
1
1796
annotation2205329
1
BBa_K592006
range2205329
1
1805
2054
BBa_R0040
1
p(tetR)
TetR repressible promoter
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Sequence for pTet inverting regulator driven by the TetR protein.</P>
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
true
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
BBa_I718015
1
BBa_I718015
ter-lox66-rbs-dapA (B. Subtilis)
David Bikard
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
false
false
_141_
0
1481
9
Released HQ 2013
In stock
false
downstream construct of the synthetic multicellular bacteria
none
none
false
component1956322
1
BBa_B0010
component1956328
1
BBa_I718016
component1956324
1
BBa_B0012
component1956335
1
BBa_I718005
component1956330
1
BBa_B0030
annotation1956330
1
BBa_B0030
range1956330
1
180
194
annotation1956328
1
BBa_I718016
range1956328
1
138
171
annotation1956335
1
BBa_I718005
range1956335
1
201
1076
annotation1956324
1
BBa_B0012
range1956324
1
89
129
annotation1956322
1
BBa_B0010
range1956322
1
1
80
BBa_S03855
1
BBa_S03855
I718017:E0840
Eimad Shotar
2007-10-25T11:00:00Z
2015-05-08T01:14:31Z
false
true
_9_
0
1568
9
Released HQ 2013
In stock
false
false
component1956431
1
BBa_B0012
component1956425
1
BBa_B0030
component1956428
1
BBa_E0040
component1956423
1
BBa_I718017
component1956429
1
BBa_B0010
annotation1956431
1
BBa_B0012
range1956431
1
880
920
annotation1956428
1
BBa_E0040
range1956428
1
64
783
annotation1956429
1
BBa_B0010
range1956429
1
792
871
annotation1956425
1
BBa_B0030
range1956425
1
43
57
annotation1956423
1
BBa_I718017
range1956423
1
1
34
BBa_B0013
1
BBa_B0013
TE from coliphage T7 (+/-)
Reshma Shetty
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream.<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
false
annotation1691
1
T7 TE
range1691
1
14
33
annotation7021
1
BBa_B0013
range7021
1
1
47
annotation1697
1
''
range1697
1
1
6
annotation1695
1
C
range1695
1
37
37
annotation1692
1
stop
range1692
1
40
40
annotation1698
1
polya
range1698
1
34
47
annotation1696
1
C
range1696
1
10
10
BBa_J61016
1
BBa_J61016
Biobrick converter for BamHI-part-EcoRI
John Anderson
2006-08-10T11:00:00Z
2015-08-31T02:02:59Z
false
false
_95_
0
483
95
Released HQ 2013
In stock
false
Inserting a BamHI/EcoRI fragment into the BglII/MfeI sites of J61015 destroys the restriction sites and introduces standard Biobrick flanking sequence. In the process, the OnRFP cassette is destroyed allowing Red to White screening of colonies.
N/A
<tt>
PCR ca1007F/R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)<br>
PCR ca1008F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)<br>
PCR ca1007F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)<br>
Sub into pSB1A2 (EcoRI/SpeI)<br>
Products are:<br>
Bca1007 NotI/BamHI transfer<br>
Bca1008 BamHI/EcoRI transfer<br>
Bca1009 NotI/EcoRI transfer<br>
------------------------------------------------
ca1007F Forward EcoRI for 5' PspOMI NotI converter<br>
ctctggaattcgcggccgcttctagagggccctccctatcagtgatagagattg<br>
ca1007R Reverse SpeI for 3' BglII BamHI converter<br>
ccgctactagtagatcttataaacgcagaaaggcccac<br>
ca1008F Forward EcoRI for 5' BglII BamHI converter<br>
ctctggaattcgcggccgcttctagaagatcttccctatcagtgatagagattg<br>
ca1009R Reverse SpeI for 3' MfeI EcoRI converter<br>
ccgctactagtcaattgtataaacgcagaaaggcccac<br>
false
BBa_K598020
1
BBa_K598020
B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE
Zhenrun ZHANG
2011-09-27T11:00:00Z
2015-05-08T01:12:50Z
false
false
_770_
0
8606
9
Released HQ 2013
In stock
false
B0015+pBAD+vioAB+B0015+pBAD+TPP ribozyme 1.20+vioD+B0015+pBAD+vioE
The Ribosomal Binding Site of vioD is contained in TPP ribozyme 1.20.
The hammerhead ribozyme comes from E. coli, and the TPP aptamer comes from B. subtilis.
false
annotation2154545
1
B0015
range2154545
1
4565
4693
annotation2154516
1
rbs
range2154516
1
273
279
annotation2154575
1
rbs
range2154575
1
4976
4982
annotation2154515
1
pBAD
range2154515
1
138
267
annotation2154586
1
vioE
range2154586
1
6489
7064
annotation2154542
1
vioB
range2154542
1
1559
4556
annotation2154517
1
vioA
range2154517
1
285
1542
annotation2154576
1
vioD
range2154576
1
4988
6112
annotation2154548
1
pBAD
range2154548
1
4702
4831
annotation2154577
1
pBAD
range2154577
1
6342
6471
annotation2154514
1
B0015
range2154514
1
1
129
annotation2154588
1
rbs
range2154588
1
6477
6483
annotation2154518
1
rbs
range2154518
1
1545
1551
annotation2154556
1
TPP ribozyme 1.20
range2154556
1
4838
4987
annotation2154583
1
B0015
range2154583
1
6205
6333
BBa_I732902
1
BBa_I732902
R0010 I732020
Zhan Jian
2007-10-16T11:00:00Z
2015-08-31T04:08:00Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1948531
1
BBa_R0010
component1948543
1
BBa_I732006
component1948546
1
BBa_B0012
component1948539
1
BBa_B0034
component1948544
1
BBa_B0010
annotation1948531
1
BBa_R0010
range1948531
1
1
200
annotation1948539
1
BBa_B0034
range1948539
1
209
220
annotation1948546
1
BBa_B0012
range1948546
1
557
597
annotation1948544
1
BBa_B0010
range1948544
1
469
548
annotation1948543
1
BBa_I732006
range1948543
1
227
460
BBa_K876068
1
BBa_K876068
Term-Term-pLsrA-LacI
Zakaraya Ashour, Sanket Shah
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
true
_1138_
0
13835
9
Released HQ 2013
In stock
false
TT-pLasrA-LacI
N/A
N/A
false
component2251336
1
BBa_B0015
component2251337
1
BBa_K117002
component2251339
1
BBa_C0012
annotation2251337
1
BBa_K117002
range2251337
1
138
239
annotation2251339
1
BBa_C0012
range2251339
1
246
1373
annotation2251336
1
BBa_B0015
range2251336
1
1
129
BBa_I715032
1
BBa_I715032
lacIq reverse
Jordan Baumgardner
2007-07-17T11:00:00Z
2015-08-31T04:07:49Z
false
false
_120_
0
1518
9
Released HQ 2013
In stock
false
This is a reverse version of BBa_C0012, the lacIq repressor cds.
Standard biobrick prefix and suffix were used. Since PCR was used, DNA sequence verification was needed - no mutations were found.
PCR was used to amplify C0012 using primers that switched the biobrick prefix and suffix.
false
BBa_K733002
1
BBa_K733002
<i>xylR</i>+<i>PxylA</i>: A xylose inducible promoter with its transcriptional regulator.
WANG, Yuqi
2012-09-15T11:00:00Z
2015-05-08T01:13:06Z
false
false
_979_
0
12444
9
Released HQ 2013
In stock
false
Not available at this moment.
Not available at this moment.
Not available at this moment.
false
annotation2193604
1
Sigma A, -35
range2193604
1
1277
1282
annotation2193589
1
xylR
range2193589
1
7
1173
annotation2193603
1
Sigma A, -10
range2193603
1
1300
1305
BBa_K769022
1
BBa_K769022
lux operon(K769011) + GFP(I13522)
Tomomi YOKOYAMA
2012-09-18T11:00:00Z
2015-05-08T01:13:14Z
false
false
_1021_
0
14294
9
Released HQ 2013
In stock
false
lux operon(K769011) + GFP(I13522)
amprify green light
P.kishitanii
false
component2186528
1
BBa_I13522
component2186513
1
BBa_K769011
annotation2186513
1
BBa_K769011
range2186513
1
1
8531
annotation2186528
1
BBa_I13522
range2186528
1
8540
9476
BBa_K567018
1
BBa_K567018
PT7-GFP-TAG-RFP
Xiaopan Ma and Xiwen Zhao
2011-09-29T11:00:00Z
2015-05-08T01:12:43Z
false
false
_735_
0
8782
9
Released HQ 2013
In stock
false
GFP and RFP linked with a flexible chain and a stop codon TAG is inserted in the flexible chain. This biobrick is under the control of T7 promoter and lac operator. This part is used to testify the function of PT7-TDRS (BBa_K567011) and tRNA(Asp)-TAG (BBa_K567013).
false
annotation2154492
1
T7 TE
range2154492
1
1712
1759
annotation2154491
1
GFP-TAG-RFP
range2154491
1
89
1621
annotation2154346
1
PT7
range2154346
1
2
18
annotation2154444
1
TAG
range2154444
1
926
928
annotation2154349
1
rbs
range2154349
1
75
81
annotation2154350
1
GFP
range2154350
1
197
910
annotation2154347
1
lac operator
range2154347
1
21
44
annotation2154469
1
mRFP
range2154469
1
944
1621
BBa_I715039
1
BBa_I715039
pLac-RBS-RFP
Oyinade Adefuye
2007-07-31T11:00:00Z
2015-08-31T04:07:49Z
false
true
_120_
0
1523
9
Released HQ 2013
In stock
false
RBS-1Chlora
RBS-1Chlora
RBS-1Chlora
false
component2245597
1
BBa_R0010
component2245609
1
BBa_I13502
annotation2245609
1
BBa_I13502
range2245609
1
209
932
annotation2245597
1
BBa_R0010
range2245597
1
1
200
BBa_K747095
1
BBa_K747095
TAL-Protein_TT6_Direpeat
Lucas Schneider
2012-09-24T11:00:00Z
2015-05-08T01:13:11Z
false
false
_998_
0
13224
9
Released HQ 2013
In stock
false
follows
This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
This Part was synthesized and flanked with BsmBI-restriction sites.
false
annotation2197073
1
TT 6
range2197073
1
14
211
BBa_K747094
1
BBa_K747094
TAL-Protein_TG6_Direpeat
Lucas Schneider
2012-09-24T11:00:00Z
2015-05-08T01:13:11Z
false
false
_998_
0
13224
9
Released HQ 2013
In stock
false
follows
This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
This Part was synthesized and flanked with BsmBI-restriction sites.
false
annotation2197072
1
TC 6
range2197072
1
14
211
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
annotation23316
1
conserved
range23316
1
7
10
BBa_P0153
1
BBa_P0153
PoPS -> cII (p22) [S0167]
Randy Rettberg
2004-04-24T11:00:00Z
2015-05-08T01:14:10Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Protein generator converting TIPS to the protein cII from p22. Used as the input section for Quad Part Inverters Q01530.
true
component944588
1
BBa_B0010
component944598
1
BBa_B0012
component944568
1
BBa_B0031
component944580
1
BBa_C0053
annotation944588
1
BBa_B0010
range944588
1
741
820
annotation944598
1
BBa_B0012
range944598
1
829
869
annotation944580
1
BBa_C0053
range944580
1
21
707
annotation944568
1
BBa_B0031
range944568
1
1
14
BBa_K741008
1
BBa_K741008
pCon(0.244)-RBS-crogfp-T
Wuyang Chen
2012-07-23T11:00:00Z
2015-05-08T01:13:09Z
false
false
_990_
0
11656
9
Released HQ 2013
In stock
true
Here, pCon(J23114) constantly expressing the fusion protein crogfp at 10% of the strength of pCon(J23100).
TBD
BBa_J23114
BBa_K741006
false
component2223132
1
BBa_J23105
component2223143
1
BBa_K741006
annotation2223132
1
BBa_J23105
range2223132
1
1
35
annotation2223143
1
BBa_K741006
range2223143
1
44
1131
BBa_J202012
1
BBa_J202012
PBAD Mutant#13
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -70 relative to the araC transcriptional start site was changed from G to A.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178468
1
araI2
range2178468
1
61
77
annotation2178467
1
araI1
range2178467
1
40
56
annotation2178466
1
Transcription Start
range2178466
1
112
112
annotation2178465
1
Mutation
range2178465
1
42
42
BBa_K849003
1
BBa_K849003
bifunctional Cyclase from Gibberella fujikuroi
Martin Schneider, Sebastian Roy
2012-09-23T11:00:00Z
2015-05-08T01:13:34Z
false
false
_1109_
0
12313
9
Released HQ 2013
In stock
true
CPS/KS
no
Genomic sequence of Gibberella fujikuroi.
false
annotation2202327
1
cds
range2202327
1
1
2859
BBa_K567008
1
BBa_K567008
PT7-Luc-2AGG
Yunfeng Ruan and Chunying Li
2011-09-28T11:00:00Z
2015-05-08T01:12:43Z
false
false
_735_
0
8782
9
Released HQ 2013
In stock
false
This biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 2 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg) (BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002).
Cell is cultured in 50ug/ml kanamycin 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA.
Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes.
Amount of bioluminescence produced can be detected using luminometer.
PCR is used to insert the 2 AGG codons into the gene after ATG.
T7 promoter is derived from pET28A. Wild type Luciferase from BBa_I712019.
false
annotation2150771
1
luciferase
range2150771
1
89
1756
annotation2150787
1
rbs
range2150787
1
75
81
annotation2150784
1
PT7
range2150784
1
2
18
annotation2150769
1
ATG
range2150769
1
89
91
annotation2150785
1
lac operator
range2150785
1
21
44
annotation2150770
1
2AGG
range2150770
1
95
100
BBa_I15016
1
ecfp
B0032.ECFP
Jeff Tabor
2005-04-14T11:00:00Z
2015-08-31T04:07:38Z
false
false
_5_
0
88
5
Released HQ 2013
In stock
false
This part accepts POPS to produce ECFP under the medium RBS B0032
true
component1478405
1
BBa_B0032
component1478408
1
BBa_E0020
annotation1478408
1
BBa_E0020
range1478408
1
20
742
annotation1478405
1
BBa_B0032
range1478405
1
1
13
BBa_K801092
1
BBa_K801092
4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
Ingmar Polte
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
false
false
_1057_
0
13150
9
Released HQ 2013
In stock
false
4-coumarate - coenzym A ligase (4CL) coding region from ''Arabidopsis thaliana'' preceeded by the yeast consensus sequence for improved expression.
-
Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
http://www.ncbi.nlm.nih.gov/nucleotide/609339/
PMID: 15932991
false
annotation2196267
1
Consensus sequence
range2196267
1
1
6
annotation2201481
1
Start codon
range2201481
1
7
9
annotation2202114
1
Stop codon
range2202114
1
1690
1692
annotation2201482
1
4CL
range2201482
1
7
1689
BBa_K784006
1
BBa_K784006
Theophylline riboswitch 12.1 + mCherry
Ilya Vainberg Slutskin
2012-09-12T11:00:00Z
2015-05-08T01:13:21Z
false
false
_1039_
0
11677
9
Released HQ 2013
In stock
false
This part is a direct fusion of the theophylline riboswitch ([[Part:BBa_K784005|BBa_K784005]]) and the mCherry protein ([[Part:BBa_J06504|BBa_J06504]]) which was amplified from [[Part:BBa_J06702|BBa_J06702]]. The part was generated by [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr]. This part was to be used in order to characterize the effect of theophylline concentration on the level of translation of the mCherry protein.
The theophylline riboswitch had to be directly fused to the start codon of the mCherry protein. To achieve this, [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr] was used.
This part is a composite part of [[Part:BBa_K784005|BBa_K784005]] and [[Part:BBa_J06504|BBa_J06504]]
false
component2182907
1
BBa_K784005
component2182910
1
BBa_J06504
annotation2182910
1
BBa_J06504
range2182910
1
75
788
annotation2182907
1
BBa_K784005
range2182907
1
1
74
BBa_S05055
1
BBa_S05055
R0010:P0451
Muyuan Chen
2012-09-17T11:00:00Z
2015-05-08T01:14:49Z
false
false
_9_
0
12559
9
Released HQ 2013
In stock
false
false
component2184443
1
BBa_R0010
component2184462
1
BBa_P0451
annotation2184462
1
BBa_P0451
range2184462
1
209
1138
annotation2184443
1
BBa_R0010
range2184443
1
1
200
BBa_I6076
1
BBa_I6076
Promoter O_H Test R0065.E0432
Caitlin Conboy and Jennifer Braff
2004-03-17T12:00:00Z
2015-08-31T04:07:43Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
false
component949378
1
BBa_B0012
component949368
1
BBa_B0010
component949360
1
BBa_E0032
component949336
1
BBa_R0065
component949348
1
BBa_B0034
annotation949378
1
BBa_B0012
range949378
1
982
1022
annotation949336
1
BBa_R0065
range949336
1
1
97
annotation949368
1
BBa_B0010
range949368
1
894
973
annotation949360
1
BBa_E0032
range949360
1
124
885
annotation949348
1
BBa_B0034
range949348
1
106
117
BBa_K863021
1
BBa_K863021
bhal laccase from Bacillus halodurans
Isabel Huber
2012-09-22T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
12844
9
Released HQ 2013
In stock
false
bhal laccase from Bacillus halodurans
test
Bacillus halodurans C-125
false
annotation2213230
1
ATG
range2213230
1
1
3
annotation2213229
1
CDS
range2213229
1
1
1503
annotation2213231
1
TAA
range2213231
1
1501
1503
BBa_K929002
1
BBa_K929002
modified AID with CMV and hGH-polyA
Potsdam Bioware 2012 iGEM
2012-09-17T11:00:00Z
2015-05-08T01:13:46Z
false
false
_1194_
0
14457
9
Released HQ 2013
In stock
false
The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K929001). It is built of 3 parts: CMV promoter (BBa_I712004), superAID and hGH polyadenylation signal sequence (BBa_K404108).<br>
AID:<br>
AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes. <br>
The AID motif is naturally terminated with the Nuclear Export Sequence (NES) that causes the protein to translocate from the nucleus to the cytoplasm. Additionally, upstream, dysfunctional Nuclear Localization Sequence (NLS) is located. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate. That is why we called it superAID<br>
Functional NLS sequence:<br>
This part of the BioBrick directs the expressed protein into the nucleus, where it can mutate stronger.
<br>
Kozak sequence<br>
Kozak consensus sequence is added upstream of the AID mutant to express the protein stronger.<br>
CMV promoter:<br>
CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter.<br>
hGH polyadenylation signal sequence:<br>
Polyadenylation is a significant part for the translation and stability of mRNA. In eukaryotes, it is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression. hGH terminator gives a signal to start polyadenylation in the translation process.
blaa bla
3 other Biobricks: CMV promoter (BBa_I712004), superAID (BBa_K929001) and hGH polyadenylation signal sequence (BBa_K404108).
false
annotation2184470
1
Kozak sequence
range2184470
1
663
677
annotation2184469
1
BBa_I712004
range2184469
1
1
654
annotation2184468
1
CMV promoter
range2184468
1
1
654
annotation2193793
1
AUG
range2193793
1
671
673
annotation2193794
1
UAA
range2193794
1
1247
1249
annotation2184472
1
mod. AID
range2184472
1
696
1250
annotation2196257
1
AgeI
range2196257
1
1241
1246
annotation2184471
1
NLS sequence
range2184471
1
678
695
annotation2184475
1
BBa_K404108
range2184475
1
1259
1736
annotation2184474
1
hGH terminator
range2184474
1
1259
1736
annotation2184473
1
BBa_K929001
range2184473
1
663
1249
BBa_K863010
1
BBa_K863010
tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag
Isabel Huber
2012-09-17T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
12844
9
Released HQ 2013
In stock
false
tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag
test
Thermus thermophilus HB27
false
annotation2184732
1
tthl
range2184732
1
34
1419
annotation2184708
1
T7 promoter
range2184708
1
1
23
annotation2184782
1
TAA
range2184782
1
1438
1440
annotation2184783
1
TAA
range2184783
1
1441
1443
annotation2184671
1
ATG
range2184671
1
34
36
annotation2184733
1
6x HIS
range2184733
1
1420
1437
BBa_I6211
1
BBa_I6211
Promoter O_H Test R0083.E0432
Jennifer Braff
2004-05-09T11:00:00Z
2015-08-31T04:07:44Z
false
false
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
false
component950781
1
BBa_E0032
component950789
1
BBa_B0010
component950799
1
BBa_B0012
component950769
1
BBa_B0034
component950764
1
BBa_R0083
annotation950781
1
BBa_E0032
range950781
1
105
866
annotation950769
1
BBa_B0034
range950769
1
87
98
annotation950789
1
BBa_B0010
range950789
1
875
954
annotation950764
1
BBa_R0083
range950764
1
1
78
annotation950799
1
BBa_B0012
range950799
1
963
1003
BBa_K778002
1
fhlA-E363
fhlA-E363G (engineered trascriptional activator fhlA with a mutation)
Yuka Yamazaki
2012-09-21T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1032_
0
13134
9
Released HQ 2013
In stock
false
fhlA363
E363G
BBa_K778001
false
annotation2194869
1
E363G mutation
range2194869
1
1088
1088
annotation2194870
1
stop
range2194870
1
2077
2079
annotation2195764
1
A to G (different from E. coli K-12 MG1655)
range2195764
1
1906
1906
annotation2194868
1
start
range2194868
1
1
1
annotation2201467
1
FhlA-E363G
range2201467
1
1
2079
BBa_K737005
1
BBa_K737005
Inducible GVPC Generator
Li Kang
2012-09-17T11:00:00Z
2015-05-08T01:13:07Z
false
false
_986_
0
14291
9
Released HQ 2013
In stock
false
This part contains a constitutive promoter (J23106), a coding region of TetR protein, a terminator (B0015), a promoter repressed by TetR protein (R0040) a coding region of GvpC and GFP and a terminator (B0015) behind it.
This part can be used to induce the strength of promoter (R0040) by Tet and indicate the content of GvpC by fluorescence of GFP. In short, it can regulate the expression of GvpC.
We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP.
To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP. Here is the curve:
We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP.
To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.
No
false
component2184861
1
BBa_K737017
component2184853
1
BBa_P0440
component2184872
1
BBa_E0840
component2184860
1
BBa_B0034
component2184854
1
BBa_R0040
component2184840
1
BBa_J23106
annotation2184861
1
BBa_K737017
range2184861
1
972
1475
annotation2184854
1
BBa_R0040
range2184854
1
892
945
annotation2184853
1
BBa_P0440
range2184853
1
44
883
annotation2184872
1
BBa_E0840
range2184872
1
1484
2361
annotation2184840
1
BBa_J23106
range2184840
1
1
35
annotation2184860
1
BBa_B0034
range2184860
1
954
965
BBa_I14044
1
BBa_I14044
weak RBS . LacI
Aditi Shrivastava, Jiwon Lee, Sarah Welch
2004-08-03T11:00:00Z
2015-08-31T04:07:38Z
false
false
_4_
0
166
7
Released HQ 2013
In stock
true
BBa_B0031 BBa_C0012
false
component1041736
1
BBa_B0031
component1041746
1
BBa_C0012
annotation1041746
1
BBa_C0012
range1041746
1
21
1148
annotation1041736
1
BBa_B0031
range1041736
1
1
14
BBa_I0466
1
BBa_I0466
RhlR Protein Generator
mit
2004-07-12T11:00:00Z
2015-08-31T04:07:29Z
false
false
_6_
0
101
7
Released HQ 2013
In stock
false
RhlR can sense N-butyryl-HSL in the media and can activate transcription from R0071. It can be used as part of a receiver device.
true
component943304
1
BBa_B0034
component943320
1
BBa_B0010
component943314
1
BBa_C0071
component943330
1
BBa_B0012
annotation943320
1
BBa_B0010
range943320
1
814
893
annotation943314
1
BBa_C0071
range943314
1
19
780
annotation943304
1
BBa_B0034
range943304
1
1
12
annotation943330
1
BBa_B0012
range943330
1
902
942
BBa_K876004
1
BBa_K876004
pTet-RBS-GFP-T-pLac-TetR
Alec Spinhirne
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13834
9
Released HQ 2013
In stock
false
Test mechanism for the effectiveness of TetR in repressing pTet. Also used to show the effect of ATc on TetR
Biobrick placement
Created from biobricks
false
component2264651
1
BBa_C0040
component2264641
1
BBa_R0010
component2264640
1
BBa_K081012
component2264625
1
BBa_R0040
annotation2264651
1
BBa_C0040
range2264651
1
1065
1749
annotation2264625
1
BBa_R0040
range2264625
1
1
54
annotation2264641
1
BBa_R0010
range2264641
1
859
1058
annotation2264640
1
BBa_K081012
range2264640
1
63
850
BBa_K778006
1
ArgBox16
ArgR binding sites (including 16 binding sites)
Yuka Yamazaki
2012-09-22T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1032_
0
13134
9
Released HQ 2013
In stock
false
BBa_K778003 ??2
sukima
biobrick part (BBa_K778003)
false
component2194871
1
BBa_K778003
component2194872
1
BBa_K778003
annotation2194871
1
BBa_K778003
range2194871
1
1
144
annotation2194872
1
BBa_K778003
range2194872
1
153
296
BBa_C0012
1
lacI
lacI repressor from E. coli (+LVA)
Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.</P> <P>A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.</P>
References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P> References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P>Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.<P> Incompatible with systems containing LacI, lactose, or IPTG.
represillator of Elowitz and Leibler (2000)
true
annotation7031
1
BBa_C0012
range7031
1
1
1128
annotation2213988
1
Help:Barcodes
range2213988
1
1129
1153
annotation1723
1
lacI-LVA
range1723
1
1
1128
annotation1722
1
LVA
range1722
1
1090
1128
BBa_K775001
1
BBa_K775001
RI7-Olfr154 Isoamylacetate chimeric GPCR
Sietske Grijseels
2012-09-04T11:00:00Z
2015-05-08T01:13:15Z
false
false
_1027_
0
12035
9
Released HQ 2013
In stock
false
BBa_K775001 encodes a chimeric isoamylacetate-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mouse Olfr154, flanked by the N- and C- terminals of the rat OR RI7.
-
N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
Ligand binding domain: Olfr154 NM_013728, Mus musculus, (mouse)
C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
false
BBa_E0036
1
EYFP
enhanced yellow fluorescent protein derived from A. victoria GFP
jcbraff
2004-06-06T11:00:00Z
2015-08-31T04:07:25Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
-- No description --
true
BBa_K909006
1
BBa_K909006
Full-length lacZ with RBS and Terminator
Isaak Mueller
2012-09-23T11:00:00Z
2015-05-08T01:13:44Z
false
false
_1174_
0
14050
9
Released HQ 2013
In stock
false
.
.
.
false
component2195194
1
BBa_I732017
component2195199
1
BBa_B0017
annotation2195199
1
BBa_B0017
range2195199
1
3102
3269
annotation2195194
1
BBa_I732017
range2195194
1
1
3093
BBa_K876009
1
BBa_K876009
LuxR-DT-pLux-TetR
Timothy Cogan
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13836
9
Released HQ 2013
In stock
false
Inducible promoter for TetR
Biobrick placement
Generated from biobricks
false
component2259761
1
BBa_R0062
component2259749
1
BBa_C0062
component2259759
1
BBa_B0015
component2259768
1
BBa_C0040
annotation2259761
1
BBa_R0062
range2259761
1
927
981
annotation2259759
1
BBa_B0015
range2259759
1
790
918
annotation2259768
1
BBa_C0040
range2259768
1
988
1672
annotation2259749
1
BBa_C0062
range2259749
1
1
756
BBa_J202014
1
BBa_J202014
PBAD Mutant#15
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -59 relative to the araC transcriptional start site was changed from C to T.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178473
1
Mutation
range2178473
1
53
53
annotation2178474
1
Transcription Start
range2178474
1
112
112
annotation2178476
1
araI2
range2178476
1
61
77
annotation2178475
1
araI1
range2178475
1
40
56
BBa_J23119
1
BBa_J23119
constitutive promoter family member
John Anderson
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
false
Later
N/A
Overlap extension of synthetic oligonucleotides
true
BBa_K876038
1
BBa_K876038
TT-pLasR-araC
Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
2012-10-01T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13835
9
Released HQ 2013
In stock
false
TT-pLasR-araC
N/A
N/A
false
component2251249
1
BBa_B0015
component2251259
1
BBa_K876044
annotation2251249
1
BBa_B0015
range2251249
1
1
129
annotation2251259
1
BBa_K876044
range2251259
1
138
1240
BBa_K936013
1
BBa_K936013
pelB-LC-Cutinase fusion protein
Mattan Hamou
2012-07-23T11:00:00Z
2015-05-08T01:13:47Z
false
false
_1201_
0
13899
9
Released HQ 2013
In stock
false
pelB leader sequence attached to enzyme LC-Cutinase protein coding sequence in order to allow the cell to bring the enzyme to the periplasm for easier secretion.
Adding sequence of pelB in front of sequence for Cuntinase
http://aem.asm.org/content/78/5/1556.long
Constructed from pelB leader sequence and protein coding sequence for LC-Cutinase
false
annotation2178085
1
BBa_K936000
range2178085
1
67
951
annotation2178083
1
PelB
range2178083
1
1
66
annotation2178084
1
BBa_J32015
range2178084
1
1
66
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
<em>V. fischeri</em>
true
annotation2046
1
-35
range2046
1
20
25
annotation2047
1
-10
range2047
1
42
47
annotation2048
1
start
range2048
1
53
53
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2045
1
LuxR/HSL
range2045
1
1
20
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_44_46_
0
24
7
Released HQ 2013
In stock
false
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_E0240
1
GFP report
GFP generator
Jennifer Braff
2004-10-17T11:00:00Z
2015-08-31T04:07:26Z
false
true
_11_1_
0
61
7
Released HQ 2013
In stock
false
B0032.E0040.B0015
true
component1249231
1
BBa_B0012
component1249213
1
BBa_B0032
component1249221
1
BBa_B0010
component1249216
1
BBa_E0040
annotation1249231
1
BBa_B0012
range1249231
1
836
876
annotation1249213
1
BBa_B0032
range1249213
1
1
13
annotation1249216
1
BBa_E0040
range1249216
1
20
739
annotation1249221
1
BBa_B0010
range1249221
1
748
827
BBa_K876013
1
BBa_K876013
LacI-RFP-ST-pLac-LuxI
Timothy Cogan
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13836
9
Released HQ 2013
In stock
false
LacI and RFP attached to a constitutive promoter for LuxI
Biobrick placement
Generated from biobricks
false
component2264696
1
BBa_R0010
component2264704
1
BBa_C0061
component2264680
1
BBa_C0012
component2264695
1
BBa_K081014
annotation2264696
1
BBa_R0010
range2264696
1
1944
2143
annotation2264695
1
BBa_K081014
range2264695
1
1162
1935
annotation2264704
1
BBa_C0061
range2264704
1
2150
2767
annotation2264680
1
BBa_C0012
range2264680
1
1
1128
BBa_K917014
1
BBa_K917014
pLac promoter plus S. oneidensis tetraheme cytochrome c cymA
Jakub Krakowiak
2012-09-23T11:00:00Z
2015-05-08T01:13:46Z
false
false
_1182_
0
13804
9
Released HQ 2013
In stock
true
This part consists of the standard registry lac promoter (BBa_J33207) S. oneidensis tetraheme cytochrome c cymA (K917009).
no special notes
pLac gene comes from E. coli BL21 genomic DNA
cymA was cloned from S. oneidensis MR-1 genomic DNA
false
component2196015
1
BBa_J33207
component2196019
1
BBa_K917009
annotation2196015
1
BBa_J33207
range2196015
1
1
600
annotation2196019
1
BBa_K917009
range2196019
1
609
1207
BBa_R0079
1
LasR+PAI
Promoter (LasR & PAI regulated)
Alvin Carter Powers (Fighting Darwins)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Binding region for LasR protein (positive regulation)
"Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
true
annotation318495
1
-35
range318495
1
117
122
annotation300992
1
OP1
range300992
1
106
125
annotation318496
1
-10
range318496
1
140
145
annotation300985
1
OP2
range300985
1
46
63
BBa_K863001
1
BBa_K863001
bpul laccase from Bacillus pumilus
Isabel Huber
2012-09-17T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
12844
9
Released HQ 2013
In stock
false
bpul (Laccase from Bacillus pumilus)
test
Bacillus pumilus DSM 27 (ATCC7061)
false
annotation2184621
1
bpul
range2184621
1
1
1533
annotation2184622
1
ATG
range2184622
1
1
3
annotation2184905
1
A (Original) - T (Mutation)
range2184905
1
801
801
annotation2184641
1
TAA
range2184641
1
1531
1533
BBa_I716107
1
PT7-E
T7 Promoter (Mutant E)
David Tulga
2007-06-08T11:00:00Z
2015-08-31T04:07:51Z
false
false
_110_
0
1694
9
Released HQ 2013
In stock
false
This is the T7 Promoter which is activated when the T7 RNA Polymerase is produced. It provides very good PoPS (Polymerases Per Second) output, as well as it is highly orthogonal to E. Coli's natural transcription system. This one of the moderately active (Mutant E) variations we are using.
{{BerkiGEM2007-Biobrick2.0Intro}}
This part was generated synthetically.
false
annotation1954611
1
T7 Promoter E
range1954611
1
1
29
BBa_K808002
1
tctA_503aa
tctA_503: large subunit A1 of the tripartite tricarboxylate transporter family
Valentina Herbring, Sebastian Palluk, Andreas Schmidt
2012-09-04T11:00:00Z
2015-05-08T01:13:25Z
false
false
_1065_
0
13606
9
Released HQ 2013
In stock
false
tctA_503: large subunit A1 of the tripartite tricarboxylate transporter family
.
Comamonas tesosteroni KF1
false
BBa_S0107
1
BBa_S0107
B0033.C0040
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-05-08T01:14:17Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component939826
1
BBa_C0040
component939815
1
BBa_B0033
annotation939826
1
BBa_C0040
range939826
1
18
677
annotation939815
1
BBa_B0033
range939815
1
1
11
BBa_S03857
1
BBa_S03857
E0840:I718016
Eimad Shotar
2007-10-25T11:00:00Z
2015-05-08T01:14:31Z
false
true
_9_
0
1568
9
Released HQ 2013
In stock
false
false
component1956449
1
BBa_B0030
component1956453
1
BBa_B0010
component1956452
1
BBa_E0040
component1956459
1
BBa_I718016
component1956455
1
BBa_B0012
annotation1956459
1
BBa_I718016
range1956459
1
887
920
annotation1956453
1
BBa_B0010
range1956453
1
750
829
annotation1956455
1
BBa_B0012
range1956455
1
838
878
annotation1956452
1
BBa_E0040
range1956452
1
22
741
annotation1956449
1
BBa_B0030
range1956449
1
1
15
BBa_I6107
1
BBa_I6107
Promoter O_H Test R0079.E0430
cconboy
2004-05-09T11:00:00Z
2015-08-31T04:07:44Z
false
false
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
false
component949926
1
BBa_E0030
component949931
1
BBa_B0010
component949924
1
BBa_B0034
component949941
1
BBa_B0012
component949919
1
BBa_R0079
annotation949941
1
BBa_B0012
range949941
1
1003
1043
annotation949931
1
BBa_B0010
range949931
1
915
994
annotation949924
1
BBa_B0034
range949924
1
166
177
annotation949926
1
BBa_E0030
range949926
1
184
906
annotation949919
1
BBa_R0079
range949919
1
1
157
BBa_J202011
1
BBa_J202011
PBAD Mutant#12
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -56 relative to the araC transcriptional start site was changed from A to G.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178461
1
Mutation
range2178461
1
56
56
annotation2178462
1
Transcription Start
range2178462
1
112
112
annotation2178463
1
araI1
range2178463
1
40
56
annotation2178464
1
araI2
range2178464
1
61
77
BBa_I13504
1
BBa_I13504
Screening plasmid intermediate
jkm
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
false
true
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction.
true
component1505067
1
BBa_B0034
component1505069
1
BBa_E0040
component1505084
1
BBa_B0012
component1505074
1
BBa_B0010
annotation1505074
1
BBa_B0010
range1505074
1
747
826
annotation1505069
1
BBa_E0040
range1505069
1
19
738
annotation1505067
1
BBa_B0034
range1505067
1
1
12
annotation1505084
1
BBa_B0012
range1505084
1
835
875
BBa_K953002
1
BBa_K953002
Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)
Daniel Winter
2012-09-23T11:00:00Z
2015-05-08T01:13:49Z
false
false
_1221_
0
14275
9
Released HQ 2013
In stock
true
Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time.
As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells.
Optimised for expression in E. coli
Agrobacterium tumefaciens
false
annotation2196368
1
RBS
range2196368
1
1
22
annotation2196369
1
Start Codon
range2196369
1
23
25
annotation2196370
1
Bacteriophytochrome
range2196370
1
26
2230
annotation2196371
1
Stop
range2196371
1
2231
2236
annotation2196372
1
BBa_K953002
range2196372
1
1
2236
BBa_I732097
1
BBa_I732097
Promoter Activity Reporter (RFP)
Zhan Jian
2007-10-16T11:00:00Z
2015-08-31T04:07:57Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
Promoter can be inserted into the adapter containing BglII and BamHI sites. The activity of inserted promoter can be quantificationally measured using ONPG assay or fluorescent assay.
N/A
N/A
false
component2253298
1
BBa_I732007
component2253300
1
BBa_B0034
component2253310
1
BBa_B0015
component2253303
1
BBa_E1010
annotation2253300
1
BBa_B0034
range2253300
1
28
39
annotation2253310
1
BBa_B0015
range2253310
1
760
888
annotation2253298
1
BBa_I732007
range2253298
1
1
19
annotation2253303
1
BBa_E1010
range2253303
1
46
751
BBa_I13457
1
BBa_I13457
Improved insertion site with two RNAse E cut sites
jkm
2005-07-18T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
Shortened RNAse E site, insertion site, duplicate RNAse E site, and a trailing 5' hairpin (HP16 from Smolke et al., 2002).
true
annotation1588510
1
RNAse E site
range1588510
1
1
21
annotation1588512
1
Insertion site
range1588512
1
22
55
annotation1588515
1
Hairpin
range1588515
1
77
111
annotation1588513
1
RNAse E site
range1588513
1
56
76
annotation1588511
1
RNAse E cut site (between the bases)
range1588511
1
9
10
annotation1588514
1
RNAse E cut site (between the bases)
range1588514
1
64
65
BBa_K863006
1
BBa_K863006
ecol laccase from E. coli
Isabel Huber
2012-09-17T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
12844
9
Released HQ 2013
In stock
false
E.coli laccase ORF
test
E. coli BL21 (DE3)
false
annotation2184435
1
ATG
range2184435
1
1
3
annotation2184436
1
TAA
range2184436
1
1549
1551
BBa_J37019
1
BBa_J37019
AHL induced LuxR generator
Deepti Aswani
2006-07-18T11:00:00Z
2015-08-31T04:08:48Z
false
false
_66_
0
1064
66
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component2245214
1
BBa_R0062
component2245220
1
BBa_B0034
component2245223
1
BBa_C0062
annotation2245223
1
BBa_C0062
range2245223
1
82
837
annotation2245220
1
BBa_B0034
range2245220
1
64
75
annotation2245214
1
BBa_R0062
range2245214
1
1
55
BBa_R0074
1
PenI
Promoter (PenI regulated)
crackdots
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
extends slightly past -50
bacillus licheniformis
true
annotation319789
1
dimer right half
range319789
1
55
76
annotation319212
1
-10
range319212
1
46
51
annotation319788
1
dimer left half
range319788
1
31
53
annotation319211
1
-35
range319211
1
23
28
annotation302713
1
PenI
range302713
1
1
77
BBa_K916000
1
BBa_K916000
TraR Coding Sequence
Joseph Elsherbini
2012-09-25T11:00:00Z
2015-05-08T01:13:46Z
false
false
_1181_
0
4259
9
Released HQ 2013
In stock
false
TraR Coding Sequence from Agrobacterium tumefaciens
-
plasmid containing TraR
false
annotation2202115
1
cds
range2202115
1
1
705
annotation2202116
1
stop
range2202116
1
703
705
annotation2202117
1
start
range2202117
1
1
3
BBa_K801012
1
BBa_K801012
ADH1 yeast terminator
Georg Sch??tzinger
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
false
false
_1057_
0
13624
9
Released HQ 2013
In stock
false
ADH1 terminator of ''S. cerevisiae''
-
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
false
annotation2202145
1
ADH1-Terminator
range2202145
1
1
349
BBa_K909015
1
BBa_K909015
p-ABA overproduction composite without promoter
Deborah Huber
2012-09-21T11:00:00Z
2015-05-08T01:13:45Z
false
false
_1174_
0
14088
9
Released HQ 2013
In stock
false
PABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of pABA. ADC synthase is composed of two subunits, PabA and PabB. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates PABA.
pabB and pabA constitute an operon that can be controled by any promoter.
S04039, K137055
false
component2193610
1
BBa_K137005
component2193606
1
BBa_B0034
component2193611
1
BBa_B0010
component2193609
1
BBa_B0034
component2193613
1
BBa_B0012
component2193607
1
BBa_K137006
annotation2193606
1
BBa_B0034
range2193606
1
1
12
annotation2193610
1
BBa_K137005
range2193610
1
1935
2519
annotation2193613
1
BBa_B0012
range2193613
1
2616
2656
annotation2193611
1
BBa_B0010
range2193611
1
2528
2607
annotation2193607
1
BBa_K137006
range2193607
1
19
1908
annotation2193609
1
BBa_B0034
range2193609
1
1917
1928
BBa_J06504
1
mCherry
monomeric RFP optimized for bacteria
ytwang
2005-07-17T11:00:00Z
2016-01-25T01:05:28Z
false
false
_20_
4206
340
20
Released HQ 2013
In stock
false
mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
<p>
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.
</p>
<p>
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
</p>
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
true
annotation1585833
1
C->T (removing PstI site)
range1585833
1
352
352
annotation1585829
1
mCherry
range1585829
1
1
711
BBa_S03877
1
BBa_S03877
B0034:I15008
Kathy Lam
2007-10-25T11:00:00Z
2015-05-08T01:14:31Z
false
false
_82_
0
927
82
Released HQ 2013
In stock
false
false
component2243953
1
BBa_I15008
component2243951
1
BBa_B0034
annotation2243953
1
BBa_I15008
range2243953
1
19
769
annotation2243951
1
BBa_B0034
range2243951
1
1
12
BBa_K812020
1
BBa_K812020
Eukaryotic PoPs -> IaaH. Indoleacetamide hydrolyase for Auxin generator, with kozak
William Rostain
2012-09-19T11:00:00Z
2015-05-08T01:13:27Z
false
true
_1069_
0
7053
9
Released HQ 2013
In stock
false
This part contains the coding sequence for Indoleacetamide hydrolyase preceded by a Kozak sequence, for expression in eukaryotic plasmids.
Kozak is included, as it cannot be added by biobrick. This part can be used to assemble biobricks for integration onto Xenopus tropicalis genome. It can also be used directly by injection into a fertilised egg, using the ready to use Xenopus plasmid BBa_K812000.
This part was created from BBa_K515100, itself from P. savastanoi (By Imperial college)
false
annotation2187776
1
IaaH
range2187776
1
12
1355
annotation2187775
1
Kozak sequence
range2187775
1
1
11
BBa_K929003
1
BBa_K929003
modified AID with CMV, hGH-polyA and eGFP
Potsdam Bioware 2012 iGEM
2012-09-17T11:00:00Z
2015-05-08T01:13:46Z
false
false
_1194_
0
14457
9
Released HQ 2013
In stock
true
The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K929001). It is built of 3 parts: CMV promoter (BBa_I712004), superAID, eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108).<br>
AID:<br>
AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes. <br>
The AID motif is naturally terminated with the Nuclear Export Sequence (NES) that causes the protein to translocate from the nucleus to the cytoplasm. Additionally, upstream, dysfunctional Nuclear Localization Sequence (NLS) is located. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate. That is why we called it superAID<br>
Functional NLS sequence:<br>
This part of the BioBrick directs the expressed protein into the nucleus, where it can mutate stronger.
<br>
Kozak sequence<br>
Kozak consensus sequence is added upstream of the AID mutant to express the protein stronger.<br>
CMV promoter:<br>
CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter.<br>
hGH polyadenylation signal sequence:<br>
Polyadenylation is a significant part for the translation and stability of mRNA. In eukaryotes, it is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression. hGH terminator gives a signal to start polyadenylation in the translation process.<br>
eGFP:<br>
In order to test where and if the AID modified sequence is functional we added to it a eGFP protein. It is used as a marker gene for detection of transfected cells, e.g. tumor cells.<br>
[[Image:UP12_biobrick3.jpg]]
4 parts: CMV promoter (BBa_I712004), superAID(BBa_K929001), eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108)
false
annotation2203642
1
NLS sequence
range2203642
1
678
696
annotation2203646
1
UAA
range2203646
1
1964
1966
annotation2203639
1
BBa_K929001
range2203639
1
663
1243
annotation2203638
1
Kozak sequence
range2203638
1
663
677
annotation2203637
1
BBa_I712004
range2203637
1
1
654
annotation2203640
1
BBa_K929004
range2203640
1
663
1966
annotation2203644
1
BBa_K404316
range2203644
1
1244
1966
annotation2203648
1
BBa_K404108
range2203648
1
1975
2453
annotation2203641
1
AUG
range2203641
1
671
673
annotation2203643
1
part of BBa_K103001
range2203643
1
697
1243
annotation2203647
1
hGH terminator
range2203647
1
1975
2453
annotation2203645
1
AgeI
range2203645
1
1958
1963
BBa_K629005
1
trkD
trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
Zilong WANG, Yi ZHENG
2011-10-03T11:00:00Z
2015-05-08T01:12:56Z
false
false
_801_
0
9022
9
Released HQ 2013
In stock
true
Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
none
ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
false
annotation2157758
1
terminator
range2157758
1
1867
1869
annotation2157757
1
rev primer
range2157757
1
1849
1869
annotation2157754
1
start
range2157754
1
1
3
annotation2157755
1
fwd primer
range2157755
1
1
21
annotation2157756
1
TrkD
range2157756
1
1
1869
BBa_K876063
1
BBa_K876063
luxI-RBS-YFP-Term-Term
Zakaraya Ashour
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
false
_1138_
0
13835
9
Released HQ 2013
In stock
false
intermediate part
N/A
N/A
false
component2259770
1
BBa_C0061
component2259786
1
BBa_E0432
annotation2259786
1
BBa_E0432
range2259786
1
652
1568
annotation2259770
1
BBa_C0061
range2259770
1
1
618
BBa_K864444
1
BBa_K864444
RFP-Linker-SYFP2
Joel ??s
2012-09-24T11:00:00Z
2015-05-08T01:13:37Z
false
false
_1124_
0
13998
9
Released HQ 2013
In stock
true
Use this part to insert any 5?????TR of your gene of interest to screen for downregulation of your gene with small RNA. PCR amplify your 5???UTR with EcoRI and BamHI and clone into any backbone carrying this part. Digest whis part with EcoRI and BamHI and as RFP is replaced, this part functions as a red/white screening system. This allows you to create a reporter system with a fluorescent marker for screening for functional small RNAs.
SYFP2 <partinfo>BBa_K864100</partinfo> is situated downstrean of the RFP <partinfo>BBa_J04450</partinfo> and is connected in frame with a 12 aa glycine-serine repeated linker <partinfo>BBa_J18922</partinfo>, to prevent folding distruptions in the YFP or the 5???UTR. The first 6 nt of the glycine-serine linker is a BamHI restriction site (GGATCC), which translates into glycine-serine in E.Coli.
When screening for working small RNAs, it's recommended to include the first 15 codons of the gene of interest.
-
Constructed from SYFP2 <partinfo>BBa_K864100</partinfo>, linker <partinfo>BBa_J18922</partinfo> and RFP <partinfo>BBa_J04450</partinfo>
false
annotation2198883
1
RFP J04450
range2198883
1
1
1068
annotation2198894
1
BamHI
range2198894
1
1070
1075
annotation2198889
1
B0012
range2198889
1
1029
1069
annotation2198886
1
B0034
range2198886
1
209
220
annotation2198896
1
SYFP2
range2198896
1
1106
1825
annotation2198885
1
Plac
range2198885
1
1
200
annotation2198890
1
B0010
range2198890
1
941
1020
annotation2198887
1
RFP
range2198887
1
227
681
annotation2198893
1
Linker
range2198893
1
1076
1105
BBa_I15017
1
ecfp
B0032.EYFP
Jeff Tabor
2005-04-14T11:00:00Z
2015-08-31T04:07:38Z
false
true
_5_
0
88
5
Released HQ 2013
In stock
false
This part accepts POPS to generate EYFP under the medium strength RBS B0032.
true
component1478422
1
BBa_E0030
component1478419
1
BBa_B0032
annotation1478422
1
BBa_E0030
range1478422
1
20
742
annotation1478419
1
BBa_B0032
range1478419
1
1
13
BBa_K850000
1
BBa_K850000
AGM1 gene from candida albicans
Min-Gyu Kim
2012-09-14T11:00:00Z
2015-05-08T01:13:34Z
false
false
_1110_
0
10645
9
Released HQ 2013
In stock
false
Phosphoacetylglucosamine mutase (N-acetylglucosamine-phosphate mutase); enzyme of UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis (1) This enzyme is part of the pathway to make the sugar subunits that make up the chitin polymer. This pathway was taken from yeast but has been shown to function in Acetobacter xylinum.
Gibson assembly fragment must have ovelapping sequences of about 50 bp.
The sequence is from the candida genome database orf19.5013 (C. albicans SC5314. It contains a ribosome binding site. It was synthesized in <500bp pieces for Gibson Assembly and assembeled into pSB1C3.
false
BBa_K801010
1
BBa_K801010
TEF2 constitutive yeast promoter
Georg Sch??tzinger
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
false
false
_1057_
0
13624
9
Released HQ 2013
In stock
false
Constitutive yeast promoter of medium strength compared to TEF1 promoter.
-
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
false
annotation2202139
1
TEF1-Promoter
range2202139
1
1
647
BBa_I13013
1
BBa_I13013
RBS Test R0040.B0032.E0130
jasonk
2004-06-30T11:00:00Z
2015-08-31T04:07:32Z
false
false
_11_6_
0
135
7
Released HQ 2013
In stock
false
Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
false
component946853
1
BBa_B0032
component946861
1
BBa_B0010
component946871
1
BBa_B0012
component946856
1
BBa_E0030
component946842
1
BBa_R0040
annotation946853
1
BBa_B0032
range946853
1
63
75
annotation946842
1
BBa_R0040
range946842
1
1
54
annotation946856
1
BBa_E0030
range946856
1
82
804
annotation946861
1
BBa_B0010
range946861
1
813
892
annotation946871
1
BBa_B0012
range946871
1
901
941
BBa_I718013
1
BBa_I718013
ter-lox66-rbs-dapA Coli
David Bikard
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
false
false
_141_
0
1481
9
Released HQ 2013
In stock
false
downstream construct of the synthetic multicellular bacterium
none
none
false
component1956316
1
BBa_B0030
component1956308
1
BBa_B0010
component1956314
1
BBa_I718016
component1956310
1
BBa_B0012
component1956321
1
BBa_I718004
annotation1956314
1
BBa_I718016
range1956314
1
138
171
annotation1956308
1
BBa_B0010
range1956308
1
1
80
annotation1956310
1
BBa_B0012
range1956310
1
89
129
annotation1956321
1
BBa_I718004
range1956321
1
201
1082
annotation1956316
1
BBa_B0030
range1956316
1
180
194
BBa_K750000
1
BBa_K750000
pBADGLT:unstable gfp expression device activated by arabinose
Sifan Wang, Yizhen Yan, Jianzhao Chi, Qingshu Wu
2012-09-17T11:00:00Z
2015-05-08T01:13:12Z
false
false
_1001_
0
14212
9
Released HQ 2013
In stock
true
This part contains Pbad promoter, RBS of 1.0 strength, GFP(lva) producer and double terminator. GFP(lva) will be produced after arabinose added. In the cell display part of our project, this part is used in part...part...part...
We use the unstable gfp for our project so the degree of fluorescence can be decreased after it get to a top point. In the cell display part, we can achieve the switch of diffrent numbers.
We built this part by using the biobricks from the dna distribution plates 2012.
false
component2184517
1
BBa_K145015
component2184511
1
BBa_B0034
component2184524
1
BBa_B0015
component2184509
1
BBa_K206000
annotation2184509
1
BBa_K206000
range2184509
1
1
130
annotation2184511
1
BBa_B0034
range2184511
1
139
150
annotation2184524
1
BBa_B0015
range2184524
1
924
1052
annotation2184517
1
BBa_K145015
range2184517
1
157
915
BBa_S05051
1
BBa_S05051
B0034:K748003
Lei Qiao
2012-09-14T11:00:00Z
2015-05-08T01:14:49Z
false
false
_9_
0
8564
9
Released HQ 2013
In stock
false
false
component2183421
1
BBa_B0034
component2183422
1
BBa_K748003
annotation2183421
1
BBa_B0034
range2183421
1
1
12
annotation2183422
1
BBa_K748003
range2183422
1
19
1404
BBa_I13458
1
BBa_I13458
promoter C and arabinose C operon
Josh Michener
2005-08-16T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left.
true
BBa_K808032
1
BBa_K808032
Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
Marie Burghard, Jascha Diemer, Philipp Rottmann, Rene Sahm, Arne Wehling
2012-09-03T11:00:00Z
2015-05-08T01:13:26Z
false
false
_1065_
0
12529
9
Released HQ 2013
In stock
false
This a a composite of 2 different BioBricks by an standard assembly, forming a scar in between.
It contains the following units:
AraC-AraO2-AraO1-AraPromo(PBAD)- RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
which is similiar to BBa_K808000 & BBa_K808030
Its a regulatory system, being induced by adding 1 to 2% of mw% L-Arabinose. It is made for regulating the cell surface Expression of our PET cleaving enzyme pNB-Est13, which is anchored c-terminal to EstA.
We were not able to add this part as an composite due to missing links and unedited registry pages. Necessary informations are featured in the sequence.
assembled GENEART synthesized product(BBa_K808030)and cloned regulatory unit (BBa_K808000), deriving from an expression system (pBAD-vector)
false
annotation2183176
1
stop
range2183176
1
4259
4261
annotation2183174
1
BBa_K808027
range2183174
1
2822
4255
annotation2183168
1
scar
range2183168
1
1211
1218
annotation2183169
1
BBa_B0029
range2183169
1
1220
1234
annotation2183171
1
BBa_K808028
range2183171
1
1241
1303
annotation2183175
1
stop
range2183175
1
4256
4258
annotation2183173
1
myctag
range2183173
1
2792
2821
annotation2183167
1
ara promotor
range2183167
1
1153
1181
annotation2183172
1
BBa_K808026
range2183172
1
1322
2791
annotation2183164
1
AraC
range2183164
1
1
879
annotation2183165
1
ara O2
range2183165
1
909
924
annotation2183170
1
6histag
range2183170
1
1304
1321
annotation2183166
1
ara O1
range2183166
1
1066
1077
BBa_K784014
1
BBa_K784014
pLux+Spacer2+RBS+mCherry
Ilya Vainberg Slutskin
2012-09-15T11:00:00Z
2015-05-08T01:13:21Z
false
false
_1039_
0
11677
9
Released HQ 2013
In stock
false
This sequence is the product of restriction cloning of the [[Part:BBa_K784009|spacer2+RBS+mCherry]] downstream to the [[Part:BBa_R0062|pLux promoter]]. In the presence of [[Part:BBa_I0462|LuxR]] transcription can be induced by [[3OC6HSL|3OC<sub>6</sub>HSL]].
No design considerations.
Cloning of the [[Part:BBa_K784009|spacer2+RBS+mCherry]] downstream to the [[Part:BBa_R0062|pLux promoter]].
false
component2183696
1
BBa_R0062
component2183708
1
BBa_K784009
annotation2183696
1
BBa_R0062
range2183696
1
1
55
annotation2183708
1
BBa_K784009
range2183708
1
64
828
BBa_P0440
1
BBa_P0440
PoPS -> TetR [S0151]
Randy Rettberg
2004-04-26T11:00:00Z
2015-05-08T01:14:10Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
true
component944886
1
BBa_B0034
component944896
1
BBa_C0040
component944912
1
BBa_B0012
component944902
1
BBa_B0010
annotation944886
1
BBa_B0034
range944886
1
1
12
annotation944902
1
BBa_B0010
range944902
1
712
791
annotation944896
1
BBa_C0040
range944896
1
19
678
annotation944912
1
BBa_B0012
range944912
1
800
840
BBa_K909007
1
BBa_K909007
TetR DNA binding domain containing BamHI site for protein fusions
Gintautas Vainorius
2012-09-22T11:00:00Z
2015-05-08T01:13:45Z
false
false
_1174_
0
14094
9
Released HQ 2013
In stock
true
Truncated version of tetracycline repressor TetR, consisting of 1 ??? 127 amino acids, containing DNA binding domain. The rest of the structure, 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression.
In addition, we introduced BamHI site at C terminus for an easy in tetR-DBD frame fusions with other proteins. Thus, it can be used in two hybrid systems for both, homo and hetero dimerizations, also one can use these fusions to turn protein-protein interaction into the repression of transcription.
Deletion of 8-10 helixes might lower the stability of protein, also TetR-DBD is no longer responsive to anhydrotetracycline
E. coli Tn10 transposon
false
annotation2194604
1
BamHI restriction site
range2194604
1
382
387
BBa_K731030
1
araC-CysEm
Inducible araC-pBAD promoter regulating M256I CysE
Jason Fontana, Daniele Rossetto
2012-08-20T11:00:00Z
2015-05-08T01:13:06Z
false
false
_977_
0
12113
9
Released HQ 2013
In stock
true
The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose.
K731200 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency. The former was cut with E&S, the latter with X&P. pSB1C3 was digested with E&P.
K731200, K731010
false
component2331178
1
BBa_K731201
component2331183
1
BBa_K731010
annotation2331183
1
BBa_K731010
range2331183
1
1212
2050
annotation2331178
1
BBa_K731201
range2331178
1
1
1203
BBa_I6101
1
BBa_I6101
Promoter O_H Test R0071.E0430
Randy Rettberg
2004-04-21T11:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component949713
1
BBa_R0071
component949720
1
BBa_E0030
component949735
1
BBa_B0012
component949718
1
BBa_B0034
component949725
1
BBa_B0010
annotation949735
1
BBa_B0012
range949735
1
899
939
annotation949713
1
BBa_R0071
range949713
1
1
53
annotation949718
1
BBa_B0034
range949718
1
62
73
annotation949720
1
BBa_E0030
range949720
1
80
802
annotation949725
1
BBa_B0010
range949725
1
811
890
BBa_I7108
1
BBa_I7108
R0053.E0840
Jennifer Braff
2005-07-27T11:00:00Z
2015-08-31T04:07:45Z
false
false
_11_
0
61
1
Released HQ 2013
In stock
false
R0053.E0840 (S_N; strong rbs; low PoPS)
false
component1596681
1
BBa_B0010
component1596676
1
BBa_E0040
component1596673
1
BBa_B0030
component1596691
1
BBa_B0012
component1596660
1
BBa_R0053
annotation1596681
1
BBa_B0010
range1596681
1
812
891
annotation1596673
1
BBa_B0030
range1596673
1
63
77
annotation1596660
1
BBa_R0053
range1596660
1
1
54
annotation1596691
1
BBa_B0012
range1596691
1
900
940
annotation1596676
1
BBa_E0040
range1596676
1
84
803
BBa_K876060
1
BBa_K876060
Term-Term-plasR-RBS-CFP-Term-Term
Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
false
_1138_
0
13835
9
Released HQ 2013
In stock
false
pLasR is off by default and can be activated by LasR.
N/A
N/A
false
component2206363
1
BBa_B0015
component2206382
1
BBa_E0422
component2206368
1
BBa_R0079
annotation2206368
1
BBa_R0079
range2206368
1
138
294
annotation2206363
1
BBa_B0015
range2206363
1
1
129
annotation2206382
1
BBa_E0422
range2206382
1
303
1219
BBa_I714036
1
BBa_I714036
Riboregulator key 1
Chunbo Lou
2007-10-24T11:00:00Z
2015-08-31T04:07:47Z
false
false
_142_
0
1911
9
Released HQ 2013
In stock
false
The same as J01008
The Isaacs key1 riboregulator taR12 is modeled on the hok/sok post-segregational killing method of plasmid R1. It makes 25 base pairs with its counterpart, the riboregulator J01010 (aka "lock 1" or "crR12").
Isaacs documentation (see reference #1):
Upon induction of the araC promoter (using 0.25% arabinose solution), the taR12 key activated the corresponding crR12 lock to express the downstream indicator, GFP, to levels of fluorescence 19-fold greater than without taR12 expression. Maximal expression was reached at 70 minutes after addition of arabinose solution, and was maintained after that point.
J01008
false
BBa_K863000
1
BBa_K863000
bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag
Isabel Huber
2012-09-17T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
12844
9
Released HQ 2013
In stock
true
bpul (Laccase from Bacillus pumilus) with T7, RBS and HIS tag
test
Bacillus pumilus DSM 27 (ATCC7061)
false
annotation2184912
1
A (Original) T (Mutation)
range2184912
1
834
834
annotation2184915
1
TAA
range2184915
1
1585
1587
annotation2184911
1
bpul
range2184911
1
34
1556
annotation2184908
1
T7- Promotor BBa_I719005
range2184908
1
1
23
annotation2184913
1
6 x HIS
range2184913
1
1557
1581
annotation2184914
1
TAA
range2184914
1
1582
1584
annotation2184909
1
RBS
range2184909
1
25
30
annotation2184910
1
ATG
range2184910
1
34
36
BBa_E0020
1
ecfp
engineered cyan fluorescent protein derived from A. victoria GFP
Caitlin Conboy and Jennifer Braff
2004-03-02T12:00:00Z
2015-08-31T04:07:25Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
true
BBa_K733012
1
BBa_K733012
<i>xylR</i>+<i>PxylA</i>+RBS+<i>ydcE</i>+<i>Ptms</i>+RBS+<i>ydcD</i>: Cell Growth Inhibition Device
WANG, Yuqi
2012-09-15T11:00:00Z
2015-05-08T01:13:06Z
false
false
_979_
0
12444
9
Released HQ 2013
In stock
true
Not available at this moment.
Not available at this moment.
Not available at this moment.
false
component2183781
1
BBa_K733010
component2183778
1
BBa_K733011
annotation2183778
1
BBa_K733011
range2183778
1
1
1764
annotation2183781
1
BBa_K733010
range2183781
1
1773
2146
BBa_K876010
1
BBa_K876010
pLux-TetR
Timothy Cogan
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13836
9
Released HQ 2013
In stock
false
Inducible promoter for TetR
Biobrick placement
Generated from biobricks
false
component2245105
1
BBa_C0040
component2245097
1
BBa_R0062
annotation2245105
1
BBa_C0040
range2245105
1
62
746
annotation2245097
1
BBa_R0062
range2245097
1
1
55
BBa_K838001
1
RO
LovTAP readout
Nicolas Gobet
2012-09-21T11:00:00Z
2015-05-08T01:13:33Z
false
false
_1097_
0
12458
9
Released HQ 2013
In stock
false
Expresses dsRed
Has 2 SpeI and 1 EcoRI site init
Nicolas Gobet
false
BBa_I732081
1
BBa_I732081
Tet repressor (RBS+ LVA- TERM-)
Zhan Jian
2007-08-02T11:00:00Z
2015-08-31T04:07:57Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
true
N/A
N/A
N/A
false
component1940758
1
BBa_B0034
component1940760
1
BBa_I732080
annotation1940758
1
BBa_B0034
range1940758
1
1
12
annotation1940760
1
BBa_I732080
range1940760
1
19
645
BBa_I13450
1
BBa_I13450
Removes BioBrick End
jkm
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
Contains both an early MfeI site and a late NsiI site. Cutting with Mfe and Spe and ligation to a BB part cut EX will form two mixed sites, neither of which can be recut. Cutting with Xba and Nsi and ligation to a BB part cut SP will again form two mixed sites, neither of which can be recut.
Should be grown in pSB1A2, as Nsi cuts both pSB2K3 and pSB1AK3 and Mfe cuts pSB4A3.
70 bp from Xba to Nsi or Mfe to Spe, the minimum for gel extraction. The middle is largely noise, to raise the size of the part.
false
annotation1494720
1
MfeI
range1494720
1
2
7
annotation1494630
1
NsiI
range1494630
1
68
73
BBa_K567001
1
BBa_K567001
lacI-Ptrc-tRNA(Arg)
Chunying Li and Yunfeng Ruan
2011-09-28T11:00:00Z
2015-05-08T01:12:43Z
false
false
_735_
0
8782
9
Released HQ 2013
In stock
true
The tRNA(Arg) is under the control of promoter tac. tRNA(Arg) expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.
The correct processing to produce mature tRNA should be ensured.
LacI- Ptac derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.
false
annotation2152783
1
Ptrc
range2152783
1
1709
1739
annotation2152782
1
lacI
range2152782
1
397
1479
annotation2152785
1
rrnB TE
range2152785
1
2054
2133
annotation2152781
1
PlacI
range2152781
1
331
367
annotation2152784
1
tRNA-Arg
range2152784
1
1785
1859
BBa_E5503
1
BBa_E5503
R0040.B0030.E0040
jcbraff
2005-05-16T11:00:00Z
2015-08-31T04:07:27Z
false
false
_1_
0
61
1
Released HQ 2013
In stock
false
R0040.B0030.E0040 (R0040.E5500)
false
component1489147
1
BBa_B0030
component1489136
1
BBa_R0040
component1489150
1
BBa_E0040
annotation1489147
1
BBa_B0030
range1489147
1
63
77
annotation1489136
1
BBa_R0040
range1489136
1
1
54
annotation1489150
1
BBa_E0040
range1489150
1
84
803
BBa_K876069
1
BBa_K876069
Term-Term-pLuxR-TetR
Zakaraya Ashour, Sanket Shah
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
true
_1138_
0
13835
9
Released HQ 2013
In stock
false
TT-pLuxR-TetR
N/A
N/A
false
component2251320
1
BBa_B0015
component2251322
1
BBa_R0062
component2251329
1
BBa_C0040
annotation2251320
1
BBa_B0015
range2251320
1
1
129
annotation2251322
1
BBa_R0062
range2251322
1
138
192
annotation2251329
1
BBa_C0040
range2251329
1
199
883
BBa_I6086
1
BBa_I6086
Promoter O_H Test R1062.E0432
Caitlin Conboy and Jennifer Braff
2004-03-17T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component949620
1
BBa_B0010
component949592
1
BBa_R1062
component949600
1
BBa_B0034
component949612
1
BBa_E0032
component949630
1
BBa_B0012
annotation949600
1
BBa_B0034
range949600
1
65
76
annotation949592
1
BBa_R1062
range949592
1
1
56
annotation949620
1
BBa_B0010
range949620
1
853
932
annotation949612
1
BBa_E0032
range949612
1
83
844
annotation949630
1
BBa_B0012
range949630
1
941
981
BBa_I732801
1
BBa_I732801
LRLa (RBS+, TERM-)
Zhan Jian
2007-10-13T11:00:00Z
2015-08-31T04:07:59Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1946165
1
BBa_B0034
component1946166
1
BBa_I732101
annotation1946165
1
BBa_B0034
range1946165
1
1
12
annotation1946166
1
BBa_I732101
range1946166
1
19
1104
BBa_I6044
1
BBa_I6044
Promoter O_H Test R0063.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948579
1
BBa_B0034
component948596
1
BBa_B0012
component948586
1
BBa_B0010
component948570
1
BBa_R0063
component948581
1
BBa_E0030
annotation948586
1
BBa_B0010
range948586
1
909
988
annotation948596
1
BBa_B0012
range948596
1
997
1037
annotation948579
1
BBa_B0034
range948579
1
160
171
annotation948581
1
BBa_E0030
range948581
1
178
900
annotation948570
1
BBa_R0063
range948570
1
1
151
BBa_K786002
1
BBa_K786002
Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
Leung Wai Tak
2012-09-20T11:00:00Z
2015-05-08T01:13:22Z
false
false
_1041_
0
14285
9
Released HQ 2013
In stock
true
Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm.
We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
Pfam (version 26.0) was used to predict the domain of fusion protein.
Linker was refereed and modified from previous research study of SRII fusion with HtrII.
From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
false
annotation2199693
1
BamHI for C-terminal fusion
range2199693
1
785
790
annotation2199691
1
HindIII for N-terminal fusin
range2199691
1
62
67
annotation2199692
1
SRII-HtrII-Tar
range2199692
1
68
2083
annotation2199690
1
RBS 1.0
range2199690
1
42
61
annotation2199689
1
Constitutive promoter BBa_J23100
range2199689
1
1
41
annotation2199694
1
B0015
range2199694
1
2117
2245
BBa_K934001
1
phaC1-A-B1
phaC1-A-B1 [P(3HB) synthesis]
Taku Nakayama
2012-09-16T11:00:00Z
2015-05-08T01:13:47Z
false
false
_1199_
0
14016
9
Released HQ 2013
In stock
true
The sequence comes from Ralstonia eutropha H16 adapted to become a standard BioBrick.
test
test
false
annotation2192329
1
RBS
range2192329
1
336
342
annotation2192326
1
phaA
range2192326
1
2207
3388
annotation2192327
1
phaB1
range2192327
1
3463
4203
annotation2192325
1
phaC1
range2192325
1
353
2122
annotation2192328
1
Pwt
range2192328
1
1
50
annotation2192331
1
RBS
range2192331
1
3453
3456
annotation2192330
1
RBS
range2192330
1
2196
2199
BBa_S03858
1
BBa_S03858
I718017:B0015
Eimad Shotar
2007-10-25T11:00:00Z
2015-05-08T01:14:31Z
false
true
_9_
0
1568
9
Released HQ 2013
In stock
false
false
component1956464
1
BBa_B0012
component1956461
1
BBa_I718017
component1956462
1
BBa_B0010
annotation1956464
1
BBa_B0012
range1956464
1
131
171
annotation1956462
1
BBa_B0010
range1956462
1
43
122
annotation1956461
1
BBa_I718017
range1956461
1
1
34
BBa_C0052
1
cI 434
cI repressor from phage 434 (+LVA)
Maia Mahoney
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.</p>
References (unparsed) here: <p>Nikolnikov,S., Posfai,G. and Sain,B. <em>The construction of a versatile plasmid vector that allows direct<br> selection of fragments cloned into six unique sites of the cI gene of coliphage 434</em>. Gene 30 (1-3), 261-265 (1984)<P> References (unparsed) here: <p>Nikolnikov,S., Posfai,G. and Sain,B. <em>The construction of a versatile plasmid vector that allows direct<br> selection of fragments cloned into six unique sites of the cI gene of coliphage 434</em>. Gene 30 (1-3), 261-265 (1984)<P><P>
Bacteriophage 434
true
annotation2213992
1
Help:Barcodes
range2213992
1
670
694
annotation1745
1
LVA
range1745
1
631
669
annotation7035
1
BBa_C0052
range7035
1
1
669
annotation1743
1
cI 434
range1743
1
1
669
BBa_K743002
1
PCaa3
PCaa3 promoter and native rbs from Synechocystis PCC6803
Juan Sim??n ??lamos
2012-09-19T11:00:00Z
2015-05-08T01:13:09Z
false
false
_994_
0
11584
9
Released HQ 2013
In stock
false
This is the 100bp sequence upstream the Caa3 gene start codon, it contains putative rbs and promoter regions.
Caa3 codes for a putative cytochrome aa3 controlling protein and its mRNA levels have shown to oscillate in a circadian manner, peaking at hour 9.
the desition of taking just 100bp upstream C
this part was PCR amplified from Synechocystis chromosome, orf sll1898.
false
annotation2188681
1
Pcaa3 promoter
range2188681
1
1
101
BBa_K934023
1
BBa_K934023
3OC6HSL->3OC12HSL
Naoki Tsukuda
2012-09-16T11:00:00Z
2015-05-08T01:13:47Z
true
false
_1199_
0
14032
9
Released HQ 2013
Discontinued
false
This part generates LasI(which generate 3OC12HSL) in the presence of 3OC6HSL.
We constructed it with (???) and (Plux,LasI part name).
test
test
false
component2184094
1
BBa_K934022
component2184114
1
BBa_S03119
component2184086
1
BBa_C0078
annotation2184114
1
BBa_S03119
range2184114
1
807
1804
annotation2184086
1
BBa_C0078
range2184086
1
85
726
annotation2184094
1
BBa_K934022
range2184094
1
1
798
BBa_K844008
1
BBa_K844008
Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized) with Met (ATG) added
Andrea Halling
2012-10-01T11:00:00Z
2016-02-10T11:28:32Z
false
false
_1104_
4206
13876
9
Released HQ 2013
In stock
false
Spider silk subunit optimized to use a reduced set of tRNA codons (1 or 2 codons per amino acid); contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.
none
Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844004
false
annotation2212463
1
Beta-spiral
range2212463
1
156
171
annotation2212460
1
Beta-spiral
range2212460
1
98
113
annotation2212465
1
Strength Domain
range2212465
1
189
207
annotation2212454
1
Beta-spiral
range2212454
1
13
27
annotation2212455
1
Beta-spiral
range2212455
1
34
48
annotation2212457
1
Beta-spiral
range2212457
1
72
87
annotation2212453
1
Elasticity Domain
range2212453
1
4
87
annotation2212458
1
Beta-helix
range2212458
1
88
97
annotation2212451
1
Start
range2212451
1
1
3
annotation2212462
1
Beta-spiral
range2212462
1
133
147
annotation2212464
1
Linker Domain
range2212464
1
172
188
annotation2212459
1
Elasticity Domain
range2212459
1
88
171
annotation2212452
1
Beta-helix
range2212452
1
4
12
annotation2212461
1
Beta-sprial
range2212461
1
117
132
annotation2212456
1
Beta-spiral
range2212456
1
49
63
BBa_K808014
1
BBa_K808014
AroY: Catalyzes protocatechuate to catechol
Sascha Hein, Sven Rumpf, Daniel Sachs, Arne Wehling, Philipp Rottmann
2012-09-01T11:00:00Z
2015-05-08T01:13:26Z
false
false
_1065_
0
11792
9
Released HQ 2013
In stock
false
-
-
-
false
BBa_R1051
1
cI lam
Promoter, Standard (lambda cI regulated)<br>
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross<br> Drew Endy<br>
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
[Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].<br><br>The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P>
<P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<br><br> Modified to comply with BBa_S0001:<br> TTGACA-17N-GATACT<br><P> Incompatible with host expressing cI repressor.
<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
true
annotation2078
1
-10
range2078
1
38
43
annotation7074
1
BBa_R1051
range7074
1
1
49
annotation2075
1
-35
range2075
1
15
20
annotation2076
1
OR1
range2076
1
25
41
annotation2077
1
OR2
range2077
1
1
17
BBa_K779504
1
BBa_K779504
Long scrambled input strand MammoBlock
Kristjan Eerik Kaseniit
2012-09-28T11:00:00Z
2015-05-08T01:13:18Z
false
false
_1033_
0
12684
9
Released HQ 2013
In stock
false
Long non-matching (scrambled) input strand to act as a negative control to the reporter formed by parts [[Part:BBa K779500]] and [[Part:BBa K779502]] or [[Part:BBa K779501]] and [[Part:BBa K779502]].
Modifications: 5' IRD800 fluorophore, 3' phosphate.
IDT DNA oligo order: /5IRD800/mAmC mUmAmA mCmCmU mAmAmC mUmAmC mAmAmC mAmAmC mAmCmU mAmUmC mCmA/3Phos/
In the domain, uses the same nucleotides as the Sa, but is scrambled so that the Sa and Sc domains are orthogonal.
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
De novo design.
false
annotation2204258
1
Toehold
range2204258
1
21
28
annotation2204257
1
Domain Sc
range2204257
1
1
20
BBa_K844000
1
BBa_K844000
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
Kathleen Miller
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
false
false
_1104_
0
9404
9
Released HQ 2013
In stock
true
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
none
Constructed through oligonucleotide annealing
false
annotation2206607
1
10x-Histidine Tag
range2206607
1
1
30
annotation2206609
1
Stop
range2206609
1
34
36
annotation2206608
1
Stop
range2206608
1
31
33
BBa_K741020
1
BBa_K741020
plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T
Wuyang Chen
2012-09-15T11:00:00Z
2015-05-08T01:13:09Z
false
false
_990_
0
11656
9
Released HQ 2013
In stock
true
We make this part in the middle of the progress of making the part plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T-pRM-anticro-T.
TBD
BBa_K741001
BBa_K741009
false
component2230908
1
BBa_K741009
component2230895
1
BBa_K741001
annotation2230895
1
BBa_K741001
range2230895
1
1
1138
annotation2230908
1
BBa_K741009
range2230908
1
1147
2277
BBa_K797000
1
BBa_K797000
tatABCD from E.coli
Tomohiro Nobeyama
2012-09-19T11:00:00Z
2015-05-08T01:13:23Z
false
false
_1053_
0
11588
9
Released HQ 2013
In stock
false
This part is involved in TAT protein export pathway of E.coli. The part consist of three genes expressing tatA,B,C proteins. They are the components of Tat complex on cell membrane, where protein with torA signal can go through
This parts have very long region so that we have to divide this parts into two regions to read sequence.
They are derived from tatABCD operon in Escherichia coli???s genome.
false
annotation2197836
1
tatD
range2197836
1
1716
2511
annotation2194797
1
tatA
range2194797
1
119
389
annotation2197721
1
tatB
range2197721
1
393
907
annotation2197737
1
tatC
range2197737
1
911
1687
BBa_K731255
1
BBa_K731255
Inducible araC-pBAD promoter with GFPmut3b, weak RBS
Daniele Rossetto
2012-08-02T11:00:00Z
2015-05-08T01:13:06Z
false
false
_977_
0
11997
9
Released HQ 2013
In stock
false
This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200) when using a weak RBS. The part is composed of part BBa_K731200 and BBa_E0240
araC-pBAD from E. coli genome, GFPmut3b is from the registry (part BBa_E0840)
false
component2331139
1
BBa_K731201
component2331150
1
BBa_E0840
annotation2331150
1
BBa_E0840
range2331150
1
1212
2089
annotation2331139
1
BBa_K731201
range2331139
1
1
1203
BBa_K876002
1
BBa_K876002
pTet-LacI
Alec Spinhirne
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13834
9
Released HQ 2013
In stock
false
Constitutive promoter for LacI, repressed by TetR
Biobrick placement
Generated from biobricks
false
component2245087
1
BBa_R0040
component2245093
1
BBa_C0012
annotation2245093
1
BBa_C0012
range2245093
1
61
1188
annotation2245087
1
BBa_R0040
range2245087
1
1
54
BBa_K847101
1
BBa_K847101
Engineered FliC with insertable expression region
Julia Borden, Michelle Yu, Bella Okiddy
2012-10-01T11:00:00Z
2015-05-08T01:13:34Z
false
false
_1107_
0
11092
9
Released HQ 2013
In stock
false
TBA
TBA
TBA
false
component2209424
1
BBa_J23100
component2209438
1
BBa_B0015
component2209431
1
BBa_K847102
component2209426
1
BBa_B0030
annotation2209438
1
BBa_B0015
range2209438
1
976
1104
annotation2209424
1
BBa_J23100
range2209424
1
1
35
annotation2209426
1
BBa_B0030
range2209426
1
44
58
annotation2209431
1
BBa_K847102
range2209431
1
65
967
BBa_I732807
1
BBa_I732807
ARL2A0103 (RBS+, TERM-)
Zhan Jian
2007-10-13T11:00:00Z
2015-08-31T04:07:59Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1946183
1
BBa_B0034
component1946184
1
BBa_I732107
annotation1946184
1
BBa_I732107
range1946184
1
19
1104
annotation1946183
1
BBa_B0034
range1946183
1
1
12
BBa_K808007
1
Operon2
putative terephtalate uptake operon2
Valentina Herbring, Sebastian Palluk, Andreas Schmidt
2012-09-04T11:00:00Z
2015-05-08T01:13:25Z
false
false
_1065_
0
12829
9
Released HQ 2013
In stock
false
putative terephtalate uptake operon2
AraProm_RBS_tctA-505_RBS_tctB-197_RBS_tphC
-
Comamonas testosteroni KF1
false
component2201309
1
BBa_J61100
component2201308
1
BBa_K808004
component2201306
1
BBa_K808000
component2201310
1
BBa_K808005
component2201311
1
BBa_J61101
component2201307
1
BBa_J61100
component2201312
1
BBa_K808001
annotation2201310
1
BBa_K808005
range2201310
1
2780
3373
annotation2201312
1
BBa_K808001
range2201312
1
3400
4368
annotation2201309
1
BBa_J61100
range2201309
1
2762
2773
annotation2201311
1
BBa_J61101
range2201311
1
3382
3393
annotation2201308
1
BBa_K808004
range2201308
1
1236
2753
annotation2201307
1
BBa_J61100
range2201307
1
1218
1229
annotation2201306
1
BBa_K808000
range2201306
1
1
1209
BBa_K649303
1
BBa_K649303
PlacIQ-RBS-ispS
Goshi Sugano
2011-09-18T11:00:00Z
2015-05-08T01:13:00Z
false
false
_826_
0
9613
9
Released HQ 2013
In stock
true
RBS+ispS is same BBa_K649400.
PlacIQ was used the part BBa_.
None.
RBS+ispS : GeneArt Product
PlacIQ : Part(BBa_)
false
component2146397
1
BBa_I14032
component2146399
1
BBa_K649400
annotation2146399
1
BBa_K649400
range2146399
1
46
1908
annotation2146397
1
BBa_I14032
range2146397
1
1
37
BBa_K909008
1
BBa_K909008
tetR-DBD-UVR8 fusion protein
Gintautas Vainorius
2012-09-22T11:00:00Z
2015-05-08T01:13:45Z
false
false
_1174_
0
14094
9
Released HQ 2013
In stock
false
Tetracycline repressor DNA binding domain fusion with truncated version of UVR8 (tetR-DBD-UVR8).
UVR8 is a UV-B sensing receptor in plants necessary for UV-B protection. In dark state UVR8 forms a dimer which is monomerized by the UV-B light (280-315 nm). A truncated version of UVR8 (14-440 amino acids) was fused with tetR DNA binding domain (tetR-DBD), lacking of dimerization domain. Such mutant shows no repression of Ptet promoter, however fusion with dimerizing protein (UVR8) returns tetR-DBD activity. Later UV-B exposure breaks UVR8 dimer and releases tetR-DBD-UVR8 from Ptet, activating transcription. Thus, this tetR-DBD-UVR8 can be used as a one step UV-B on switch.
Contains two illegal PstI sites in UVR8 coding region and BamHI site was used for protein fusion
tetR-DBD was cloned from E.coli tetracyclin repressor tetR from transposon Tn10
cDNA of UVR8 was provided by Roman Ulm, University of Geneva.
false
annotation2194628
1
tetR-DBD
range2194628
1
1
390
annotation2194629
1
UVR8
range2194629
1
391
1674
BBa_P0152
1
BBa_P0152
PoPS -> cI (434) [S0166]
Randy Rettberg
2004-04-24T11:00:00Z
2015-05-08T01:14:10Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Protein generator converting TIPS to the protein cI from 434. Used as the input section for Quad Part Inverter Q01520.
true
component944535
1
BBa_B0031
component944562
1
BBa_B0012
component944545
1
BBa_C0052
component944552
1
BBa_B0010
annotation944552
1
BBa_B0010
range944552
1
723
802
annotation944545
1
BBa_C0052
range944545
1
21
689
annotation944562
1
BBa_B0012
range944562
1
811
851
annotation944535
1
BBa_B0031
range944535
1
1
14
BBa_I732018
1
BBa_I732018
lacZ alpha fragment (RBS+ TERM-)
Zhan Jian
2007-07-13T11:00:00Z
2015-08-31T04:07:56Z
false
true
_156_
0
1557
9
Released HQ 2013
In stock
true
None
None
I732006 inserted into pSB1A2-B0034
false
component1938196
1
BBa_B0034
component1938200
1
BBa_I732006
annotation1938200
1
BBa_I732006
range1938200
1
19
252
annotation1938196
1
BBa_B0034
range1938196
1
1
12
BBa_E0032
1
YFP
enhanced yellow fluorescent protein derived from A. victoria GFP
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
2003-01-31T12:00:00Z
2015-08-31T04:07:25Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. </P> Annotated mutation cause a Q81L mutation that appears to not be near the active site. </P>
<P> <P>BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
Modified from <bb_part>BBa_E0031</bb_part>
true
annotation2156
1
YFP (LVA)
range2156
1
1
762
annotation2159
1
2
range2159
1
757
762
annotation2161
1
A (Q->L)
range2161
1
242
242
annotation7041
1
BBa_E0032
range7041
1
1
762
annotation2160
1
SsrA
range2160
1
718
756
BBa_K786003
1
BBa_K786003
Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
Leung Wai Tak
2012-09-20T11:00:00Z
2015-05-08T01:13:22Z
false
false
_1041_
0
14285
9
Released HQ 2013
In stock
true
Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm.
We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
Pfam (version 26.0) was used to predict the domain of fusion protein.
Linker was refereed and modified from previous research study of SRI fusion with HtrII.
From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
false
annotation2199719
1
B0015
range2199719
1
2029
2158
annotation2190185
1
Constitutive promoter BBa_J23100
range2190185
1
1
41
annotation2190186
1
RBS 1.0
range2190186
1
42
61
annotation2199706
1
SRI-HtrI-Tar
range2199706
1
68
1992
BBa_K737033
1
BBa_K737033
galK is the leader sequence of galactokinase (GalK)
Peiran Zhang
2012-09-17T11:00:00Z
2015-05-08T01:13:07Z
false
false
_986_
0
14291
9
Released HQ 2013
In stock
false
galK is the leader sequence of galactokinase (GalK),and Spot42 sRNA controls the synthesis of the galactokinase (GalK) in response to the availability of glucose in the environment,by blocking the recognition of antiSD motif to RBS.The repression is modest(about 2.6 fold),maybe there is no subsequent degradation caused by the major degradosome,RNaesE and RNaseIII.
Note:The ClaI site is blocked by GATC methylation due to a mistake.It should be amplified by PCR to get rid of dam methylation before ClaI degestion
No
Reference:
[1] Johannes H. Urban and Jo?? rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3
[2] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008
false
BBa_I718014
1
BBa_I718014
rbs-dapA (B. Subtilis)
David Bikard
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
false
false
_141_
0
1481
9
Released HQ 2013
In stock
true
none
none
none
false
component1956192
1
BBa_B0030
component1956197
1
BBa_I718005
annotation1956192
1
BBa_B0030
range1956192
1
1
15
annotation1956197
1
BBa_I718005
range1956197
1
22
897
BBa_K561000
1
BBa_K561000
Pvgb+YFP+tetR
HUA Yijie, PI Ruoxi, XIA Yu
2011-09-28T11:00:00Z
2015-05-08T01:12:41Z
false
false
_729_
0
8637
9
Released HQ 2013
In stock
false
The expression of YFP and tetR is under the control of microaerobic promoter vgb. The expression of tetR will repress Ptet.
Because vgb promoter induces high expression under microaerobic conditions and induces lower expression under anaerobic conditions, we used medium RBS to resist it from inducing expression under anaerobic conditions.
vgb promoter comes from Vitreoscilla sp.
false
component2146980
1
BBa_P0440
component2146967
1
BBa_I15017
component2146962
1
BBa_K561001
annotation2146962
1
BBa_K561001
range2146962
1
1
135
annotation2146967
1
BBa_I15017
range2146967
1
144
885
annotation2146980
1
BBa_P0440
range2146980
1
894
1733
BBa_K895002
1
BBa_K895002
Salty_VgrG
Frank Sargent
2012-09-21T11:00:00Z
2015-05-08T01:13:41Z
false
false
_1160_
0
8083
9
Released HQ 2013
In stock
true
This is a coding part comprising the full open reading frame of the gene encoding the <i>Salmonella enterica</i> LT2 VgrG protein (also known as STM0279 or TssI). VgrG is an important component of bacterial Type VI Secretion Systems and is believed to locate at the very tip of the Type VI secretion device. Normally, therefore, this protein would be considered to be a secreted protein and to be located outside of the bacterial cell.
The <i>Salmonella vgrG</i> gene did not require any modification to meet RFC[10] standard.
Amplified by PCR from <i>Salmonella</i> LT2 genomic DNA.
false
annotation2192729
1
vgrG
range2192729
1
1
2193
BBa_K775010
1
BBa_K775010
Isoamylacetate Chimeric GPCR with Fus1-EGFP reporter (2)
Sietske Grijseels
2012-09-23T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1027_
0
12035
9
Released HQ 2013
In stock
false
-
-
-
false
component2195596
1
BBa_K775004
component2195597
1
BBa_K775001
annotation2195596
1
BBa_K775004
range2195596
1
1
1166
annotation2195597
1
BBa_K775001
range2195597
1
1175
2597
BBa_K731520
1
BBa_K731520
IPTG inducible GFPmut3b
Francesco Guzzonato
2012-08-21T11:00:00Z
2015-05-08T01:13:06Z
false
false
_977_
0
12142
9
Released HQ 2013
In stock
true
This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control.
lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.
This construct has ben obtained from ligation between BBa_K731500 and BBa_E0240.
false
component2181566
1
BBa_E0240
component2181555
1
BBa_K731500
annotation2181566
1
BBa_E0240
range2181566
1
1248
2123
annotation2181555
1
BBa_K731500
range2181555
1
1
1239
BBa_K759012
1
BBa_K759012
eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.
Kenta Mine
2012-09-19T11:00:00Z
2015-05-08T01:13:13Z
false
false
_1010_
0
10980
9
Released HQ 2013
In stock
false
In the presence of L-arabinose, pBAD promoter is induced and then wildtype Antigen 43 (Ag43) protein and eCFP (engineered cyan fluorescent protein)is produced.
The wildtype Ag43 have 6 PstI sites.
eCFP locates in the upstream of Ag43 for estimating the relationship between expressed protein amount and aggregation efficiency.
6 PstI sites exists in Ag43 coding site so we assembled this composite part without using PstI.
Ag43(BBA_K346007) was derived from E. coli K-12 strain by Peking 2010 iGEM.
false
component2188732
1
BBa_B0015
component2188721
1
BBa_E0020
component2188718
1
BBa_I0500
component2188725
1
BBa_K346007
component2188723
1
BBa_B0034
component2188720
1
BBa_B0034
annotation2188723
1
BBa_B0034
range2188723
1
1968
1979
annotation2188720
1
BBa_B0034
range2188720
1
1219
1230
annotation2188718
1
BBa_I0500
range2188718
1
1
1210
annotation2188725
1
BBa_K346007
range2188725
1
1986
5105
annotation2188732
1
BBa_B0015
range2188732
1
5114
5242
annotation2188721
1
BBa_E0020
range2188721
1
1237
1959
BBa_K850002
1
BBa_K850002
UAP1 gene from candida albicans
Min-Gyu Kim
2012-09-14T11:00:00Z
2015-05-08T01:13:34Z
false
false
_1110_
0
10645
9
Released HQ 2013
In stock
false
UDP-N-acetylglucosamine pyrophosphorylase, catalyzes biosynthesis of UDP-N-acetylglucosamine from UTP and N-acetylglucosamine 1-phosphate; functional homolog of S. cerevisiae Qri1p; alkaline upregulated. This gene is part of the pathway that synthesizes the sugar subunit of the polymer chitin.
Part was synthesized from <500bp overlapping Gibson Assembly fragments
candida genome database orf19.4265 (C. albicans SC5314)
false
BBa_R0084
1
OmpR
Promoter (OmpR, positive)
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
NC_000913 E. coli K12
true
annotation301313
1
F3 OmpR
range301313
1
51
68
annotation301309
1
F2 OmpR
range301309
1
31
48
annotation301317
1
-35
range301317
1
73
78
annotation301318
1
-10
range301318
1
88
93
annotation301308
1
F1 OmpR
range301308
1
11
28
BBa_I746104
1
BBa_I746104
P2 promoter in agr operon from S. aureus
Zhizhen Zhao
2007-08-30T11:00:00Z
2015-08-31T04:08:04Z
false
false
_116_
0
2121
9
Released HQ 2013
In stock
false
The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the expression level from P2 promoter.
Codon usage has been optimised for E. coli (which also seems to give good results for B. subtilis
The section of the sequence constituting the P2 promoter is subject to rather more debate than the sequences of the coding regions. [http://jb.asm.org/cgi/content/full/186/22/7549 Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region J. Bacteriol. 2004 186: 7549-7555] shows the areas of the sequence to which phosphorylated AgrA binds while L Rao, R K Karls, and M J Betley: In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609???2614. shows the transcription start sites; we took the overall extent of the promoter region from that labelled in Morfeldt, E., K. Tegmark, and S. Arvidson. 1996. Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227???1237. (not available online).
true
BBa_K876073
1
BBa_K876073
pTetR-LasR-Term-Term-pLasR-LuxS-RBS-CFP-Term-Term-pLuxR-TetR
Zakaraya Ashour, Sanket Shah
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
false
_1138_
0
13835
9
Released HQ 2013
In stock
false
pTetR-LasR-TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR
N/A
N/A
false
component2298670
1
BBa_R0079
component2298671
1
BBa_K091109
component2298694
1
BBa_C0040
component2298685
1
BBa_E0422
component2298687
1
BBa_R0062
component2298665
1
BBa_K876057
annotation2298685
1
BBa_E0422
range2298685
1
1674
2590
annotation2298687
1
BBa_R0062
range2298687
1
2599
2653
annotation2298665
1
BBa_K876057
range2298665
1
1
978
annotation2298694
1
BBa_C0040
range2298694
1
2660
3344
annotation2298671
1
BBa_K091109
range2298671
1
1150
1665
annotation2298670
1
BBa_R0079
range2298670
1
987
1143
BBa_I732912
1
BBa_I732912
Promoter Adapter + LacI
Zhan Jian
2007-10-16T11:00:00Z
2015-08-31T04:08:00Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1948770
1
BBa_I732100
component1948771
1
BBa_B0010
component1948769
1
BBa_B0034
component1948767
1
BBa_I732007
component1948773
1
BBa_B0012
annotation1948771
1
BBa_B0010
range1948771
1
1140
1219
annotation1948769
1
BBa_B0034
range1948769
1
28
39
annotation1948773
1
BBa_B0012
range1948773
1
1228
1268
annotation1948770
1
BBa_I732100
range1948770
1
46
1131
annotation1948767
1
BBa_I732007
range1948767
1
1
19
BBa_I718011
1
BBa_I718011
rbs-dapA coli
David Bikard
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
false
false
_141_
0
1481
9
Released HQ 2013
In stock
true
none
none
none
false
component1956157
1
BBa_B0030
component1956162
1
BBa_I718004
annotation1956162
1
BBa_I718004
range1956162
1
22
903
annotation1956157
1
BBa_B0030
range1956157
1
1
15
BBa_B0012
1
BBa_B0012
TE from coliphageT7
Reshma Shetty
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Transcription terminator for the <i>E.coli</i> RNA polymerase.
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
false
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_I13542
1
BBa_I13542
Intermediate in two promoter screening plasmid
Josh Michener
2005-08-21T11:00:00Z
2015-08-31T04:07:35Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
-- No description --
false
component1663933
1
BBa_E1010
component1663939
1
BBa_B0010
component1663949
1
BBa_B0012
component1663921
1
BBa_I13457
component1663926
1
BBa_B0034
component1663901
1
BBa_I13453
annotation1663901
1
BBa_I13453
range1663901
1
1
130
annotation1663949
1
BBa_B0012
range1663949
1
1078
1118
annotation1663921
1
BBa_I13457
range1663921
1
139
249
annotation1663933
1
BBa_E1010
range1663933
1
276
956
annotation1663926
1
BBa_B0034
range1663926
1
258
269
annotation1663939
1
BBa_B0010
range1663939
1
990
1069
BBa_I13541
1
BBa_I13541
Intermediate in two promoter screening plasmid
Josh Michener
2005-08-21T11:00:00Z
2015-08-31T04:07:35Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
-- No description --
false
component1663874
1
BBa_B0034
component1663876
1
BBa_E0040
component1663869
1
BBa_I13453
component1663881
1
BBa_B0010
component1663891
1
BBa_B0012
annotation1663891
1
BBa_B0012
range1663891
1
973
1013
annotation1663881
1
BBa_B0010
range1663881
1
885
964
annotation1663869
1
BBa_I13453
range1663869
1
1
130
annotation1663876
1
BBa_E0040
range1663876
1
157
876
annotation1663874
1
BBa_B0034
range1663874
1
139
150
BBa_I6042
1
BBa_I6042
Promoter O_H Test R0062.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948544
1
BBa_E0030
component948559
1
BBa_B0012
component948549
1
BBa_B0010
component948542
1
BBa_B0034
component948534
1
BBa_R0062
annotation948534
1
BBa_R0062
range948534
1
1
55
annotation948542
1
BBa_B0034
range948542
1
64
75
annotation948559
1
BBa_B0012
range948559
1
901
941
annotation948544
1
BBa_E0030
range948544
1
82
804
annotation948549
1
BBa_B0010
range948549
1
813
892
BBa_K598019
1
BBa_K598019
B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
Zhenrun ZHANG
2011-09-26T11:00:00Z
2015-05-08T01:12:50Z
false
false
_770_
0
8606
9
Released HQ 2013
In stock
false
B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
the Ribosomal Binding Site of vioE is contained in TPP Ribozyme.
Hammerhead Ribozyme backbone comes from E.coli, and TPP aptamer comes from B.subtilis.
false
annotation2144630
1
rbs
range2144630
1
6122
6129
annotation2144624
1
TPP ribozyme 2.5
range2144624
1
5985
6134
annotation2144597
1
vioA
range2144597
1
285
1542
annotation2144599
1
vioB
range2144599
1
1559
4556
annotation2144595
1
pBAD
range2144595
1
138
267
annotation2144600
1
vioD
range2144600
1
4579
5704
annotation2144598
1
rbs
range2144598
1
1545
1551
annotation2144601
1
B0015
range2144601
1
5712
5841
annotation2144593
1
B0015
range2144593
1
1
129
annotation2144596
1
rbs
range2144596
1
273
279
annotation2144594
1
rbs
range2144594
1
4565
4571
annotation2144602
1
pBAD
range2144602
1
5849
5978
annotation2144631
1
vioE
range2144631
1
6135
6710
BBa_I13025
1
BBa_I13025
mnt QPI Intermediate
jenmitch
2004-07-14T11:00:00Z
2015-08-31T04:07:32Z
false
false
_6_
0
159
7
Released HQ 2013
In stock
false
-- No description --
false
component939411
1
BBa_B0034
component939424
1
BBa_C0072
annotation939411
1
BBa_B0034
range939411
1
1
12
annotation939424
1
BBa_C0072
range939424
1
19
306
BBa_S0110
1
BBa_S0110
B0033.C0052
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-05-08T01:14:17Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component939884
1
BBa_C0052
component939873
1
BBa_B0033
annotation939884
1
BBa_C0052
range939884
1
18
686
annotation939873
1
BBa_B0033
range939873
1
1
11
BBa_K863005
1
BBa_K863005
ecol laccase from E. coli with T7 promoter, RBS and His-tag
Isabel Huber
2012-09-17T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
12844
9
Released HQ 2013
In stock
true
E.coli laccase ORF with T7, RBS and HIS tag
TEST
E. coli BL21 (DE3)
false
annotation2184564
1
TAA
range2184564
1
1600
1602
annotation2184563
1
TAA
range2184563
1
1603
1605
annotation2184561
1
T7 promoter
range2184561
1
1
23
annotation2184562
1
ATG
range2184562
1
34
36
annotation2184566
1
ecol
range2184566
1
34
1581
annotation2187301
1
RBS
range2187301
1
25
30
annotation2184565
1
6x HIS
range2184565
1
1582
1599
BBa_I13528
1
BBa_I13528
Screening plasmid v1.0 intermediate
Josh Michener
2005-07-28T11:00:00Z
2015-08-31T04:07:35Z
false
true
_11_
0
253
6
Released HQ 2013
In stock
false
Intermediate in screening plasmid v2 construction.
false
component2251413
1
BBa_I13457
component2251425
1
BBa_I13507
annotation2251425
1
BBa_I13507
range2251425
1
120
980
annotation2251413
1
BBa_I13457
range2251413
1
1
111
BBa_I9026
1
BBa_I9026
rhlI translational unit
crackdots
2004-01-27T12:00:00Z
2015-08-31T04:08:11Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
true
false
component939656
1
BBa_C0070
component939646
1
BBa_B0034
annotation939646
1
BBa_B0034
range939646
1
1
12
annotation939656
1
BBa_C0070
range939656
1
19
660
BBa_K519030
1
BBa_K519030
Photocontrol device
Chifuku Mita
2011-09-27T11:00:00Z
2015-05-08T01:12:33Z
false
false
_684_
0
9626
9
Released HQ 2013
In stock
false
a
a
a
false
component2273731
1
BBa_B0015
component2273719
1
BBa_I15009
component2273714
1
BBa_I15008
component2273721
1
BBa_B0034
component2273712
1
BBa_B0034
component2273716
1
BBa_B0034
component2273724
1
BBa_I15010
annotation2273724
1
BBa_I15010
range2273724
1
1597
3859
annotation2273721
1
BBa_B0034
range2273721
1
1579
1590
annotation2273714
1
BBa_I15008
range2273714
1
19
769
annotation2273716
1
BBa_B0034
range2273716
1
778
789
annotation2273731
1
BBa_B0015
range2273731
1
3868
3996
annotation2273712
1
BBa_B0034
range2273712
1
1
12
annotation2273719
1
BBa_I15009
range2273719
1
796
1570
BBa_K750012
1
TD0.01
TIME DELAY0.01:LuxI(RBS0.01)->LuxR->LuxPR->GFP
Sifan Wang,Ruosang Qiu,Jianzhao Chi,Muxin Yu
2012-09-18T11:00:00Z
2015-05-08T01:13:12Z
false
false
_1001_
0
14212
9
Released HQ 2013
In stock
false
This part contains part8 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
editing...
We built this part by biobricks from the DNA distribution kit plates 2012.
false
component2312814
1
BBa_K750007
component2312777
1
BBa_K750005
annotation2312777
1
BBa_K750005
range2312777
1
1
935
annotation2312814
1
BBa_K750007
range2312814
1
944
2963
BBa_I13452
1
BBa_I13452
Adds another BioBrick site
jkm
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.
70 bp from Eco to Spe or Xba to Pst, to allow for gel extraction. The middle portion is largely noise, intended as filler to raise the size of the part.
true
annotation1891620
1
BbsI site
range1891620
1
8
13
annotation1891619
1
Future Eco site
range1891619
1
1
6
annotation1891621
1
BstXI site
range1891621
1
41
52
annotation1891622
1
Future Pst site
range1891622
1
44
49
BBa_I6066
1
BBa_I6066
Promoter O_H Test R0051.E0432
Caitlin Conboy and Jennifer Braff
2004-03-17T12:00:00Z
2015-08-31T04:07:43Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
false
component949082
1
BBa_R0051
component949102
1
BBa_E0032
component949090
1
BBa_B0034
component949110
1
BBa_B0010
component949120
1
BBa_B0012
annotation949082
1
BBa_R0051
range949082
1
1
49
annotation949110
1
BBa_B0010
range949110
1
846
925
annotation949120
1
BBa_B0012
range949120
1
934
974
annotation949090
1
BBa_B0034
range949090
1
58
69
annotation949102
1
BBa_E0032
range949102
1
76
837
BBa_I6056
1
BBa_I6056
Promoter O_H Test R1062.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948824
1
BBa_B0012
component948799
1
BBa_R1062
component948809
1
BBa_E0030
component948814
1
BBa_B0010
component948807
1
BBa_B0034
annotation948824
1
BBa_B0012
range948824
1
902
942
annotation948814
1
BBa_B0010
range948814
1
814
893
annotation948807
1
BBa_B0034
range948807
1
65
76
annotation948809
1
BBa_E0030
range948809
1
83
805
annotation948799
1
BBa_R1062
range948799
1
1
56
BBa_K909014
1
BBa_K909014
Constitutive expression of pABA
Deborah Huber
2012-09-21T11:00:00Z
2015-05-08T01:13:45Z
false
false
_1174_
0
14088
9
Released HQ 2013
In stock
false
PABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of pABA.
ADC synthase is composed of two subunits, PabA and PabB. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates PABA.
xyz
Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli. V K Viswanathan,; J M Green and; B P Nichols.
false
component2193599
1
BBa_B0012
component2193595
1
BBa_B0034
component2193597
1
BBa_B0010
component2193592
1
BBa_B0034
component2193596
1
BBa_K137005
component2193593
1
BBa_K137006
component2193590
1
BBa_J23100
annotation2193599
1
BBa_B0012
range2193599
1
2659
2699
annotation2193593
1
BBa_K137006
range2193593
1
62
1951
annotation2193590
1
BBa_J23100
range2193590
1
1
35
annotation2193595
1
BBa_B0034
range2193595
1
1960
1971
annotation2193592
1
BBa_B0034
range2193592
1
44
55
annotation2193597
1
BBa_B0010
range2193597
1
2571
2650
annotation2193596
1
BBa_K137005
range2193596
1
1978
2562
BBa_K801093
1
BBa_K801093
4-coumarate--coenzyme A ligase (4CL) coding region
Ingmar Polte
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
false
false
_1057_
0
13150
9
Released HQ 2013
In stock
false
4-coumarate--coenzyme A ligase (4CL) coding region from ''Arabidopsis thaliana''.
-
Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
http://www.ncbi.nlm.nih.gov/nucleotide/609339/
PMID: 15932991
false
annotation2202140
1
Start codon
range2202140
1
1
3
annotation2202143
1
4CL
range2202143
1
1
1683
annotation2202141
1
Stop codon
range2202141
1
1684
1686
BBa_I13502
1
mRFP1
Screening plasmid intermediate
jkm
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction.
true
component1505045
1
BBa_E1010
component1505038
1
BBa_B0034
annotation1505038
1
BBa_B0034
range1505038
1
1
12
annotation1505045
1
BBa_E1010
range1505045
1
19
699
BBa_I6106
1
BBa_I6106
Promoter O_H Test R0078.E0430
cconboy
2004-05-09T11:00:00Z
2015-08-31T04:07:44Z
false
false
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
false
component949885
1
BBa_R0078
component949892
1
BBa_E0030
component949907
1
BBa_B0012
component949890
1
BBa_B0034
component949897
1
BBa_B0010
annotation949892
1
BBa_E0030
range949892
1
252
974
annotation949907
1
BBa_B0012
range949907
1
1071
1111
annotation949897
1
BBa_B0010
range949897
1
983
1062
annotation949885
1
BBa_R0078
range949885
1
1
225
annotation949890
1
BBa_B0034
range949890
1
234
245
BBa_K763005
1
BBa_K763005
pADH2 + Gene encoding YAP1 protein
Luisa Mar??a Mart??nez S??nchez
2012-09-16T11:00:00Z
2015-05-08T01:13:13Z
false
false
_1014_
0
11712
9
Released HQ 2013
In stock
false
There are two transcriptional factors which regulates the ADH2 promoter: ADR1 a carbon source-responsibe zinc-finger2 and CAT83. In the absence of glucose both cis-acting sites in the promoter are bound cooperatively by the transcriptional activators. ADR1 binds to the UAS1 site while CAT8 binds to the UAS2/CSRE site and regulates positively the transcription of YAP1 protein by the activation of the ADH2 promoter. The presence of glucose downregulates the levels of the transcription factors (ADR1 and CAT8) which results in the depletion of the production of YAP1 protein by the repression of the ADH2 promoter.
No design considerations needed.
The sequences of the promoter and the gene which encodes the YAP1 protein comes from yeast Sacharomyces cerevisiae genome.
false
BBa_K863020
1
BBa_K863020
bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag
Isabel Huber
2012-09-18T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
12844
9
Released HQ 2013
In stock
false
bhal laccase from Bacillus halodurans with T7 and HIS
test
Bacillus halodurans C-125
false
annotation2202256
1
TAA
range2202256
1
1552
1554
annotation2202253
1
ATG
range2202253
1
34
36
annotation2202254
1
bhal
range2202254
1
34
1533
annotation2202257
1
TAA
range2202257
1
1555
1557
annotation2202251
1
T7
range2202251
1
1
23
annotation2202255
1
6 x HIS
range2202255
1
1534
1551
annotation2202252
1
RBS
range2202252
1
25
30
BBa_K808004
1
tctA_505aa
tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
Valentina Herbring, Sebastian Palluk, Andreas Schmidt
2012-09-04T11:00:00Z
2015-05-08T01:13:25Z
false
false
_1065_
0
12829
9
Released HQ 2013
In stock
false
tctA_505: large subunit A2 of the tripartite tricarboxylate transporter family
deletion of PstI restriction site
Comamonas testosteroni KF1
false
BBa_E0024
1
ECFP
enhanced cyan fluorescent protein derived from A. victoria GFP
jcbraff
2004-06-06T11:00:00Z
2015-08-31T04:07:25Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
-- No description --
true
BBa_R0063
1
lux pL
Promoter (luxR & HSL regulated -- lux pL)<br>
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl</p>
<P> <P> This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <p><img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"><P>Unspecified.
<em>V. fischeri.</em>
true
annotation2053
1
-35
range2053
1
89
94
annotation7071
1
BBa_R0063
range7071
1
1
151
annotation2052
1
-10
range2052
1
115
122
annotation2054
1
start
range2054
1
128
128
annotation2055
1
Putative LuxR/HSL
range2055
1
130
149
annotation2051
1
LuxR/HSL
range2051
1
1
20
BBa_S0109
1
BBa_S0109
B0033.C0051
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-05-08T01:14:17Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component939856
1
BBa_B0033
component939867
1
BBa_C0051
annotation939856
1
BBa_B0033
range939856
1
1
11
annotation939867
1
BBa_C0051
range939867
1
18
767
BBa_I732101
1
LRLa
LRLa
Zhan Jian
2007-10-13T11:00:00Z
2015-08-31T04:07:57Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
true
BBa_S03859
1
BBa_S03859
B0015:I718016
Eimad Shotar
2007-10-25T11:00:00Z
2015-05-08T01:14:31Z
false
true
_9_
0
1568
9
Released HQ 2013
In stock
false
false
component1956469
1
BBa_B0010
component1956471
1
BBa_B0012
component1956475
1
BBa_I718016
annotation1956475
1
BBa_I718016
range1956475
1
138
171
annotation1956471
1
BBa_B0012
range1956471
1
89
129
annotation1956469
1
BBa_B0010
range1956469
1
1
80
BBa_K574005
1
BBa_K574005
3OC12HSL and YFP regulated by pBad
Lei Wei
2011-09-28T11:00:00Z
2015-05-08T01:12:44Z
false
false
_745_
0
8529
9
Released HQ 2013
In stock
false
pBAD + lasI + yfp.
Empty.
Exsiting Parts.
false
component2268871
1
BBa_I13453
component2268889
1
BBa_E0430
component2268879
1
BBa_K081009
annotation2268889
1
BBa_E0430
range2268889
1
835
1712
annotation2268879
1
BBa_K081009
range2268879
1
139
826
annotation2268871
1
BBa_I13453
range2268871
1
1
130
BBa_I714031
1
BBa_I714031
OriT-R (Origin of Transfer for the R751-plasmid nic region)
Mingzhi Qu
2007-10-20T11:00:00Z
2015-08-31T04:07:47Z
false
false
_142_
0
2248
9
Released HQ 2013
In stock
false
OriT-R751 (Origin of Transfer for the R751-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by R751 plasmid machinery begins via rolling circle replication.
Enterobacter aerogenes plasmid R751
NCBI:NC_001735
true
BBa_K936000
1
BBa_K936000
LC Cutinase
Nicholas Csicsery
2012-07-16T11:00:00Z
2015-05-08T01:13:47Z
false
false
_1201_
0
10185
9
Released HQ 2013
In stock
false
This is the LC Cutinase gene used to break PET into terephthalic acid and ethylene glycol.
Eliminated BioBrick restriction sites and added a TAA double stop.
Modified from the paper Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach by Sulaiman et al.
false
BBa_K779503
1
BBa_K779503
Long input strand MammoBlock
Kristjan Eerik Kaseniit
2012-09-28T11:00:00Z
2015-05-08T01:13:18Z
false
false
_1033_
0
12684
9
Released HQ 2013
In stock
false
Long matching input strand to the reporter formed by parts [[Part:BBa K779500]] and [[Part:BBa K779502]] or [[Part:BBa K779501]] and [[Part:BBa K779502]].
Modifications: 5' IRD800 fluorophore, 3' phosphate.
IDT DNA oligo order: /5IRD800/mCmA mCmCmC mAmCmU mCmCmU mCmUmC mCmCmA mCmCmA mAmCmU mAmUmC mCmA/3Phos/
3' phosphate added to protect from some 3' exonucleases (suggested by IDT).
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
De novo design.
false
annotation2204255
1
Domain Sa
range2204255
1
1
20
annotation2204256
1
Toehold
range2204256
1
21
28
BBa_K784047
1
BBa_K784047
Theophylline riboswitch + wt T7 RNAP
Asaf Blumental
2012-09-25T11:00:00Z
2015-05-08T01:13:22Z
false
false
_1039_
0
11677
9
Released HQ 2013
In stock
true
This part contains an engineered theophylline riboswitch (RS) ([http://partsregistry.org/Part:BBa_K784005 BBa K784005]) and T7 RNA polymerase ([http://partsregistry.org/Part:BBa_I2032 BBa I2032]). The riboswitch contains a RBS and it will be exposed and starts translating the T7 RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7 RNAP are adjacent to one another without any restriction site between them. There is an additional stop codon at the end of the gene.
The riboswitch had to fused to the RNAP using assembly PCR.
Assembly PCR of [http://partsregistry.org/Part:BBa_K784005 BBa K784005] and [http://partsregistry.org/Part:BBa_I2032 BBa_I2032].
[http://partsregistry.org/Part:BBa_I2032 BBa I2032] was received from Ann K. Ganesan.
false
component2199521
1
BBa_I2032
component2199519
1
BBa_K784005
annotation2199521
1
BBa_I2032
range2199521
1
75
2729
annotation2199519
1
BBa_K784005
range2199519
1
1
74
BBa_K907000
1
Bxb1_Int
Mycobacterium Phage Bxb1 gp35, DNA integrase
Dong-hui Choe, Soo-in Lee
2012-09-19T11:00:00Z
2015-05-08T01:13:44Z
false
false
_1172_
0
12628
9
Released HQ 2013
In stock
true
This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1.
The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences.
We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655).
When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.
Internal restriction endonuclease site is changed(two PstI sites, one XbaI sites) without changing amino acid sequence.
This part is derived by Mycobacterium phage Bxb1. And synthesized by Bioneer.
http://www.ncbi.nlm.nih.gov/protein/NP_075302.1
false
annotation2187533
1
Mycobacterium phage Bxb1 integrase CDS
range2187533
1
1
1503
BBa_J202013
1
BBa_J202013
PBAD Mutant#14
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -69 relative to the araC transcriptional start site was changed from C to A.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178470
1
Transcription Start
range2178470
1
112
112
annotation2178469
1
Mutation
range2178469
1
43
43
annotation2178472
1
araI2
range2178472
1
61
77
annotation2178471
1
araI1
range2178471
1
40
56
BBa_I716106
1
PT7-D
T7 Promoter (Mutant D)
David Tulga
2007-06-08T11:00:00Z
2015-08-31T04:07:51Z
false
false
_110_
0
1694
9
Released HQ 2013
In stock
false
This is the T7 Promoter which is activated when the T7 RNA Polymerase is produced. It provides very good PoPS (Polymerases Per Second) output, as well as it is highly orthogonal to E. Coli's natural transcription system. This one of the moderately active (Mutant D) variations we are using.
{{BerkiGEM2007-Biobrick2.0Intro}}
This part was generated synthetically.
false
annotation1954612
1
T7 Promoter D
range1954612
1
1
29
BBa_C0040
1
tetR
tetracycline repressor from transposon Tn10 (+LVA)
June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P>
References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
true
annotation23329
1
tetR
range23329
1
4
620
annotation23330
1
SsrA
range23330
1
621
654
annotation2213989
1
Help:Barcodes
range2213989
1
661
685
BBa_I714044
1
BBa_I714044
[R0010][I714031]
Mingzhi Qu
2007-10-20T11:00:00Z
2015-08-31T04:07:47Z
false
false
_142_
0
2248
9
Released HQ 2013
In stock
false
This is I714031 with R0010 Promoter in the front.
[R0010]+[I714031]
false
component1951661
1
BBa_I714031
component1951654
1
BBa_R0010
annotation1951654
1
BBa_R0010
range1951654
1
1
200
annotation1951661
1
BBa_I714031
range1951661
1
209
486
BBa_K777113
1
BBa_K777113
motA
Team Goettingen
2012-09-16T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
Released HQ 2013
In stock
false
The motA gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGACCGGTGGAAGCCTTGG <br>
**Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATGCTTCCTCGGTTGTCG
* The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
false
annotation2184144
1
stop
range2184144
1
831
831
annotation2184143
1
start
range2184143
1
1
1
annotation2184142
1
MotA coding sequence
range2184142
1
1
831
BBa_K775000
1
BBa_K775000
RI7-GPR109A Niacin chimeric GPCR
Sietske Grijseels
2012-08-30T11:00:00Z
2015-05-08T01:13:15Z
false
false
_1027_
0
12035
9
Released HQ 2013
In stock
false
BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.
Promoter: GPD
Terminator: CYC1.
Homo sapiens NP_808219.1
Rat
false
annotation2181639
1
BamHI restriction site
range2181639
1
113
118
annotation2181642
1
FLAGtag
range2181642
1
128
151
annotation2181645
1
NdeI restriction site
range2181645
1
1157
1162
annotation2181638
1
GPD promoter
range2181638
1
1
112
annotation2181643
1
N terminus RI7
range2181643
1
152
337
annotation2181640
1
Kozak sequence
range2181640
1
119
124
annotation2181646
1
CYC1 terminator
range2181646
1
1163
1414
annotation2181644
1
Stopcodon
range2181644
1
1151
1156
annotation2181647
1
C terminus RI7
range2181647
1
1055
1150
annotation2181648
1
Niacin receptor part
range2181648
1
338
1054
annotation2181641
1
Startcodon
range2181641
1
125
127
BBa_I6040
1
BBa_I6040
Promoter O_H Test R0053.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948509
1
BBa_E0030
component948507
1
BBa_B0034
component948514
1
BBa_B0010
component948524
1
BBa_B0012
component948497
1
BBa_R0053
annotation948524
1
BBa_B0012
range948524
1
900
940
annotation948507
1
BBa_B0034
range948507
1
63
74
annotation948509
1
BBa_E0030
range948509
1
81
803
annotation948497
1
BBa_R0053
range948497
1
1
54
annotation948514
1
BBa_B0010
range948514
1
812
891
BBa_E0840
1
GFP genera
GFP generator
Jennifer Braff
2004-10-17T11:00:00Z
2015-08-31T04:07:26Z
false
true
_11_1_
0
61
7
Released HQ 2013
In stock
true
B0030.E0040.B0015
true
component1249242
1
BBa_E0040
component1249257
1
BBa_B0012
component1249247
1
BBa_B0010
component1249239
1
BBa_B0030
annotation1249239
1
BBa_B0030
range1249239
1
1
15
annotation1249257
1
BBa_B0012
range1249257
1
838
878
annotation1249242
1
BBa_E0040
range1249242
1
22
741
annotation1249247
1
BBa_B0010
range1249247
1
750
829
BBa_K864680
1
BBa_K864680
tetM
Erik Lundin
2012-09-25T11:00:00Z
2015-05-08T01:13:37Z
false
false
_1124_
0
9827
9
In stock
false
tetM
-
-
false
annotation2214190
1
tetM
range2214190
1
1
1920
BBa_K876000
1
BBa_K876000
pLac-TetR
Alec Spinhirne
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13834
9
Released HQ 2013
In stock
false
Constitutive producer for TetR, repressed by LacI
Biobrick placement
Created from biobricks
false
component2245076
1
BBa_R0010
component2245086
1
BBa_C0040
annotation2245086
1
BBa_C0040
range2245086
1
207
891
annotation2245076
1
BBa_R0010
range2245076
1
1
200
BBa_I13001
1
BBa_I13001
RBS B0030 w/ YFP (-LVA) TT
Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
2004-06-27T11:00:00Z
2015-08-31T04:07:32Z
false
false
_6_
0
101
7
Released HQ 2013
In stock
false
B0030. E0030. B0015
true
component943431
1
BBa_E0030
component943436
1
BBa_B0010
component943446
1
BBa_B0012
component943428
1
BBa_B0030
annotation943431
1
BBa_E0030
range943431
1
22
744
annotation943436
1
BBa_B0010
range943436
1
753
832
annotation943446
1
BBa_B0012
range943446
1
841
881
annotation943428
1
BBa_B0030
range943428
1
1
15
BBa_I13600
1
BBa_I13600
Tet with CFP reporter (without LVA tag)
Christopher Batten, Victoria Chou, Kenneth Nesmith
2004-07-15T11:00:00Z
2015-08-31T04:07:35Z
false
true
_6_
0
101
7
Released HQ 2013
In stock
false
-- No description --
true
component947192
1
BBa_B0034
component947194
1
BBa_E0020
component947199
1
BBa_B0010
component947184
1
BBa_R0040
component947209
1
BBa_B0012
annotation947209
1
BBa_B0012
range947209
1
900
940
annotation947194
1
BBa_E0020
range947194
1
81
803
annotation947199
1
BBa_B0010
range947199
1
812
891
annotation947184
1
BBa_R0040
range947184
1
1
54
annotation947192
1
BBa_B0034
range947192
1
63
74
BBa_B0023
1
BBa_B0023
TE from coliphage T7, reversed
Caitlin Conboy
2003-10-16T11:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
This part is the reverse of terminator B0013, a bacteriophage T7 terminator which was engineered to be bidirectional. Contains a poly A insertion upstream of a 20 bp stem-loop.
false
BBa_K849004
1
BBa_K849004
ent-Kaurene Oxidase from Gibberella fujikuroi
Martin Schneider
2012-09-23T11:00:00Z
2015-05-08T01:13:34Z
false
false
_1109_
0
12313
9
Released HQ 2013
In stock
false
KO
no
Genomic Sequence from Gibberella fujikuroi
false
annotation2202568
1
cds
range2202568
1
1
1578
BBa_I763007
1
pLambda+RF
promoter lambda (cI regulated) with RFP reporter
Alice Pasini
2007-07-29T11:00:00Z
2015-08-31T04:08:08Z
false
false
_111_
0
2088
9
Released HQ 2013
In stock
false
Promoter lambda - cI regulated - (R0051) with RFP (E1010) as a reporter. It can be used as a detector of the glucose metabolism.
Reporter
Igem 2007 plates
true
component2252869
1
BBa_R0051
component2252884
1
BBa_I13507
annotation2252869
1
BBa_R0051
range2252869
1
1
49
annotation2252884
1
BBa_I13507
range2252884
1
58
918
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
<em>V. fischeri</em> <genbank>AF170104</genbank>
true
annotation1766
1
luxR
range1766
1
1
750
annotation1765
1
A
range1765
1
492
492
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation1764
1
T
range1764
1
174
174
annotation1762
1
prefix
range1762
1
1
2
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
BBa_K773002
1
BBa_K773002
Proteorhodopsin
Katie Knister
2012-09-30T11:00:00Z
2015-05-08T01:13:15Z
false
false
_1025_
0
11646
9
Released HQ 2013
In stock
false
This part carries the gene for a proteorhodopsin pump, which can generate a proton motive force for making ATP.
We codon optimized the gene for E. coli and eliminated four restriction sites.
Synthesized with gene assembly
false
annotation2209515
1
Start
range2209515
1
1
3
annotation2209517
1
stop
range2209517
1
772
774
annotation2209514
1
Proteorhodopsin
range2209514
1
1
777
annotation2209516
1
stop
range2209516
1
775
777
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_K876052
1
BBa_K876052
plasR-luxI-RBS-YFP-Term-Term
Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
false
_1138_
0
13835
9
Released HQ 2013
In stock
false
pLasR is off by default and can be actated by LasR. LuxI converts SAM to AHL.
N/A
N/A
false
component2264743
1
BBa_E0432
component2264725
1
BBa_R0079
component2264727
1
BBa_C0061
annotation2264743
1
BBa_E0432
range2264743
1
815
1731
annotation2264727
1
BBa_C0061
range2264727
1
164
781
annotation2264725
1
BBa_R0079
range2264725
1
1
157
BBa_R0078
1
cinR
Promoter (cinR and HSL regulated)
Drew Endy
2004-01-29T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Rhizobium leguminosarum
true
annotation318250
1
stem_loop
range318250
1
91
136
annotation318234
1
unidentified/uncharacterized CinR dependent
range318234
1
1
220
annotation318244
1
stem_loop
range318244
1
35
74
BBa_K741002
1
BBa_K741002
plac-RBS-GFP-T
Wuyang Chen
2012-07-23T11:00:00Z
2015-05-08T01:13:09Z
false
false
_990_
0
11656
9
Released HQ 2013
In stock
true
Different strength of green fluorescence will be generated determined by the concentration of IPTG.
TBD
BBa_R0010
BBa_E0040
false
component2177894
1
BBa_I13504
component2177878
1
BBa_R0010
annotation2177878
1
BBa_R0010
range2177878
1
1
200
annotation2177894
1
BBa_I13504
range2177894
1
209
1083
BBa_I6084
1
BBa_I6084
Promoter O_H Test R1053.E0432
Caitlin Conboy and Jennifer Braff
2004-03-17T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component949552
1
BBa_B0034
component949564
1
BBa_E0032
component949582
1
BBa_B0012
component949542
1
BBa_R1053
component949572
1
BBa_B0010
annotation949542
1
BBa_R1053
range949542
1
1
55
annotation949552
1
BBa_B0034
range949552
1
64
75
annotation949572
1
BBa_B0010
range949572
1
852
931
annotation949582
1
BBa_B0012
range949582
1
940
980
annotation949564
1
BBa_E0032
range949564
1
82
843
BBa_S0117
1
BBa_S0117
B0031.C0053
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-05-08T01:14:17Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component939989
1
BBa_B0031
component940001
1
BBa_C0053
annotation940001
1
BBa_C0053
range940001
1
21
707
annotation939989
1
BBa_B0031
range939989
1
1
14
BBa_I6032
1
BBa_I6032
Promoter O_H Test R0040.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948347
1
BBa_R0040
component948355
1
BBa_B0034
component948357
1
BBa_E0030
component948372
1
BBa_B0012
component948362
1
BBa_B0010
annotation948347
1
BBa_R0040
range948347
1
1
54
annotation948372
1
BBa_B0012
range948372
1
900
940
annotation948357
1
BBa_E0030
range948357
1
81
803
annotation948362
1
BBa_B0010
range948362
1
812
891
annotation948355
1
BBa_B0034
range948355
1
63
74
BBa_K909003
1
BBa_K909003
Cph8 (Cph1-Envz-Fusionprotein) with RBS
Isaak Mueller
2012-09-23T11:00:00Z
2015-05-08T01:13:44Z
false
false
_1174_
0
14050
9
Released HQ 2013
In stock
false
.
.
.
false
annotation2194863
1
conserved
range2194863
1
5
8
annotation2194865
1
cph8 (cph1-envZ-fusion)
range2194865
1
21
2255
annotation2194864
1
BBa_B0034
range2194864
1
1
12
annotation2194867
1
BBa_K909002
range2194867
1
21
2255
annotation2194866
1
g->a
range2194866
1
128
128
BBa_K909009
1
BBa_K909009
cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana
Gintautas Vainorius
2012-09-22T11:00:00Z
2015-05-08T01:13:45Z
false
false
_1174_
0
14094
9
Released HQ 2013
In stock
true
UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection.
In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain), to use UVR8 as a UV-B sensing domain.
Contains two illegal PstI sites.
cDNA of UVR8 from Arabidopsis thaliana was provided by Roman Ulm, University of Geneva.
false
annotation2202567
1
BamHI site
range2202567
1
4
9
BBa_I716211
1
BBa_I716211
Barnase active peptide
Samantha Liang
2007-06-04T11:00:00Z
2015-08-31T04:07:51Z
false
true
_110_
0
933
52
Released HQ 2013
In stock
false
Barnase active peptide (no start codon)
PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716211
sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg
sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716211
sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg
sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
false
BBa_K801071
1
BBa_K801071
7-methylxanthine N-methyltransferase CaMXMT1-strep
Roman Prechtl
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
false
false
_1057_
0
11966
9
Released HQ 2013
In stock
false
7-methylxanthine N-methyltransferase CaMXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
-
Synthesized by IDT, Yeast codon optimized
false
annotation2198586
1
Start codon
range2198586
1
7
9
annotation2198148
1
Strep Tag
range2198148
1
1141
1170
annotation2197709
1
CaMXMT1
range2197709
1
7
1140
annotation2197876
1
Consensus sequence
range2197876
1
1
6
annotation2198587
1
Stop codon
range2198587
1
1171
1176
BBa_K895005
1
BBa_K895005
Salty_VgrG-CD27_endolysin
Frank Sargent
2012-09-22T11:00:00Z
2015-05-08T01:13:41Z
false
false
_1160_
0
8083
9
Released HQ 2013
In stock
false
This is a coding part comprising the full open reading frame of <i>Salmonella enterica</i> LT2 <i>vgrG</i> gene (also known as STM0289 or <i>tssI</i>) linked <i>via</i> an haemagglutinin eptitope tag to a synthetic gene encoding an endolysin from the CD27 bacteriophage. The CD27 endolysin is believed to have high specificity against the cell wall of <i>Clostridium difficile</i>.
None.
The <i>vgrG</i> section is derived from <partinfo>BBa_K895002</partinfo>, which was used as a PCR template, and the endolysin section is derived from a synthetic gene designed by Neil Fairweather's group at Imperial College London.
false
annotation2194046
1
endolysin
range2194046
1
2221
2793
annotation2194045
1
HA tag
range2194045
1
2188
2214
annotation2194044
1
vgrG
range2194044
1
1
2187
BBa_K779501
1
BBa_K779501
Long reporter top strand MammoBlock
Kristjan Eerik Kaseniit
2012-09-28T11:00:00Z
2015-05-08T01:13:18Z
false
false
_1033_
0
12684
9
Released HQ 2013
In stock
true
Reporter top strand.
Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmU mCmUmC mCmCmA mCmCmA /3AlexF488N/
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature.
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
De novo design.
false
annotation2204235
1
Domain Sa
range2204235
1
1
20
BBa_S0100
1
BBa_S0100
B0034.C0012
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-05-08T01:14:16Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
false
component939698
1
BBa_B0034
component939708
1
BBa_C0012
annotation939708
1
BBa_C0012
range939708
1
19
1146
annotation939698
1
BBa_B0034
range939698
1
1
12
BBa_I746324
1
BBa_I746324
GFP under control of Psid promoter
Stefan Milde
2007-09-10T11:00:00Z
2015-08-31T04:08:04Z
false
false
_116_
0
2122
9
Released HQ 2013
In stock
false
This part is used to screen the level of induction of the Psid promoter (I746364).
The Psid promoter is induced by a class of phage activators (P2 ogr: I746350, PSP3pag: I746351, phiR73 delta: I746352).
The Psid promoter was put upstream of I13504.
registry (I13504) and own construct (I746364)
false
component1943843
1
BBa_B0010
component1943842
1
BBa_E0040
component1943840
1
BBa_B0034
component1943845
1
BBa_B0012
component1943838
1
BBa_I746364
annotation1943845
1
BBa_B0012
range1943845
1
936
976
annotation1943840
1
BBa_B0034
range1943840
1
102
113
annotation1943843
1
BBa_B0010
range1943843
1
848
927
annotation1943838
1
BBa_I746364
range1943838
1
1
93
annotation1943842
1
BBa_E0040
range1943842
1
120
839
BBa_J202009
1
BBa_J202009
PBAD Mutant#10
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -59 relative to the araC transcriptional start site was changed from C to T. Also, C at -48 was changed to T.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by random mutagenesis.
false
annotation2178454
1
Transcription Start
range2178454
1
112
112
annotation2178456
1
araI2
range2178456
1
61
77
annotation2178452
1
Mutation 1
range2178452
1
53
53
annotation2178453
1
Mutation 2
range2178453
1
64
64
annotation2178455
1
araI1
range2178455
1
40
56
BBa_K733018
1
BBa_K733018
<i>xylR</i>+<i>PxylA</i>+RBS+GFP+Double Terminator
WANG, Yuqi
2012-09-15T11:00:00Z
2015-05-08T01:13:07Z
false
false
_979_
0
12444
9
Released HQ 2013
In stock
true
Not available at this moment.
Not available at this moment.
Not available at this moment.
false
component2183842
1
BBa_K733002
component2183853
1
BBa_E0240
annotation2183853
1
BBa_E0240
range2183853
1
1396
2271
annotation2183842
1
BBa_K733002
range2183842
1
1
1387
BBa_K844006
1
BBa_K844006
Spider Silk 1x Subunit "W" with Met (ATG) start codon
Federico Carlos Rodriguez
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
false
false
_1104_
0
13908
9
Released HQ 2013
In stock
false
Spider silk subunit native sequence; contains two elasticity domains and one strength domain, contains added Met (ATG) on 5??? end.
none
Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844002
false
annotation2212396
1
Beta-spiral
range2212396
1
133
147
annotation2212389
1
Beta-spiral
range2212389
1
34
48
annotation2212391
1
Beta-spiral
range2212391
1
72
87
annotation2212397
1
Beta-spiral
range2212397
1
156
171
annotation2212390
1
Beta-spiral
range2212390
1
49
63
annotation2212388
1
Beta-spiral
range2212388
1
13
27
annotation2212394
1
Beta-spiral
range2212394
1
98
113
annotation2212395
1
Beta-spiral
range2212395
1
117
132
annotation2212387
1
Beta-helix
range2212387
1
4
12
annotation2212392
1
Beta-helix
range2212392
1
88
97
annotation2212385
1
Start
range2212385
1
1
3
annotation2212386
1
Elasticity Domain
range2212386
1
4
87
annotation2212398
1
Linker Domain
range2212398
1
172
188
annotation2212393
1
Elasticity Domain
range2212393
1
88
171
annotation2212399
1
Strength Domain
range2212399
1
189
207
BBa_I714033
1
BBa_I714033
crRNA-lock3-var1 (A new "lock" designed by our team)
Mingzhi Qu
2007-10-21T11:00:00Z
2015-08-31T04:07:47Z
false
false
_142_
0
2248
9
Released HQ 2013
In stock
false
crRNA(I714033) and taRNA(I714034) whose structure resemble that of J23078 (lock3) and J23066 (key3) but with a different matching regions to minimize the possible interaction with the key3-lock3.
true
BBa_I6070
1
BBa_I6070
Promoter O_H Test R0053.E0432
Caitlin Conboy and Jennifer Braff
2004-03-17T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component949196
1
BBa_B0034
component949216
1
BBa_B0010
component949226
1
BBa_B0012
component949186
1
BBa_R0053
component949208
1
BBa_E0032
annotation949186
1
BBa_R0053
range949186
1
1
54
annotation949196
1
BBa_B0034
range949196
1
63
74
annotation949216
1
BBa_B0010
range949216
1
851
930
annotation949226
1
BBa_B0012
range949226
1
939
979
annotation949208
1
BBa_E0032
range949208
1
81
842
BBa_K574006
1
BBa_K574006
3OC12HSL regulated by pTet and pBad
Lei Wei
2011-09-28T11:00:00Z
2015-05-08T01:12:44Z
false
false
_745_
0
8529
9
Released HQ 2013
In stock
true
Part 2/3 of a Oscillator.
Empty.
Existing Parts.
false
component2295507
1
BBa_K574005
component2295487
1
BBa_K574004
annotation2295487
1
BBa_K574004
range2295487
1
1
797
annotation2295507
1
BBa_K574005
range2295507
1
806
2517
BBa_R0065
1
cI+luxR
Promoter (lambda cI and luxR regulated -- hybrid)
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. </P> <P> </P> <table width="75%" border="1"> <tr> <td><strong>LuxI</strong></td> <td><strong>cI</strong></td> <td><strong>activity of promoter</strong></td> </tr> <tr> <td>+</td> <td>+</td> <td>zero</td> </tr> <tr> <td>+</td> <td>-</td> <td>maximum</td> </tr> <tr> <td>-</td> <td>+</td> <td>zero</td> </tr> <tr> <td>-</td> <td>-</td> <td>leaky (no quantitative information)</td> </tr> </table> <P> </P>
<P> <P>This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>. <P>
true
annotation1986780
1
OR1 cI
range1986780
1
81
97
annotation1986779
1
-35
range1986779
1
71
76
annotation1986774
1
BBa_R0065
range1986774
1
1
97
annotation1986778
1
lux p(R) start
range1986778
1
58
58
annotation1986781
1
-10
range1986781
1
94
97
annotation1986776
1
-10
range1986776
1
47
52
annotation1986777
1
OR2 cI
range1986777
1
57
73
annotation1986775
1
Lux Box
range1986775
1
6
25
BBa_P0151
1
BBa_P0151
PoPS -> cI (lambda) [S0165]
Randy Rettberg
2004-04-24T11:00:00Z
2015-05-08T01:14:10Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Protein generator converting TIPS to the protein cI from lambda. Used as the input section for Quad Part Inverters Q01150 and Q01151.
true
component944529
1
BBa_B0012
component944519
1
BBa_B0010
component944503
1
BBa_B0031
component944513
1
BBa_C0051
annotation944503
1
BBa_B0031
range944503
1
1
14
annotation944513
1
BBa_C0051
range944513
1
21
770
annotation944529
1
BBa_B0012
range944529
1
892
932
annotation944519
1
BBa_B0010
range944519
1
804
883
BBa_I13519
1
BBa_I13519
Screening Plasmid Intermediate
jkm
2005-06-05T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction.
false
component1505591
1
BBa_B0012
component1505565
1
BBa_I13452
component1505576
1
BBa_E0040
component1505573
1
BBa_B0032
component1505581
1
BBa_B0010
annotation1505581
1
BBa_B0010
range1505581
1
808
887
annotation1505591
1
BBa_B0012
range1505591
1
896
936
annotation1505565
1
BBa_I13452
range1505565
1
1
52
annotation1505576
1
BBa_E0040
range1505576
1
80
799
annotation1505573
1
BBa_B0032
range1505573
1
61
73
BBa_R0071
1
RhlR+C4
Promoter (RhlR & C4-HSL regulated)
Ronny Krashinsky
2004-01-24T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.
<p>
This is the natural sequence taken from Pseudomonas aeruginosa.
<p>
Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
The -10 and -35 sites are labeled as identified in [Pearson97].
<P>
The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
<i>Pseudomonas aeruginosa</i> rhlAB promoter
true
annotation297103
1
-35
range297103
1
19
24
annotation297104
1
-10
range297104
1
42
47
annotation297102
1
RhlR
range297102
1
1
20
annotation297105
1
start
range297105
1
53
53
BBa_J202004
1
BBa_J202004
PBAD Mutant#5
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -71 relative to the araC transcriptional start site was changed from A to G.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178435
1
Mutation
range2178435
1
41
41
annotation2178434
1
Transcription Start
range2178434
1
112
112
annotation2178432
1
araI1
range2178432
1
40
56
annotation2178433
1
araI2
range2178433
1
61
77
BBa_K769020
1
BBa_K769020
lux operon(K769011) + lumP(K769019)
Tomomi YOKOYAMA
2012-09-18T11:00:00Z
2015-05-08T01:13:14Z
false
false
_1021_
0
14294
9
Released HQ 2013
In stock
false
Promoter(Pbad)-lux operon-DT + lumP(K769019)
blue shift
P.kishitanii
false
component2186406
1
BBa_K769011
component2186418
1
BBa_K769019
annotation2186406
1
BBa_K769011
range2186406
1
1
8531
annotation2186418
1
BBa_K769019
range2186418
1
8540
9301
BBa_I6105
1
BBa_I6105
Promoter O_H Test R0077.E0430
Randy Rettberg
2004-04-21T11:00:00Z
2015-08-31T04:07:44Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component949853
1
BBa_R0077
component949860
1
BBa_E0030
component949865
1
BBa_B0010
component949858
1
BBa_B0034
component949875
1
BBa_B0012
annotation949865
1
BBa_B0010
range949865
1
989
1068
annotation949860
1
BBa_E0030
range949860
1
258
980
annotation949858
1
BBa_B0034
range949858
1
240
251
annotation949853
1
BBa_R0077
range949853
1
1
231
annotation949875
1
BBa_B0012
range949875
1
1077
1117
BBa_S03156
1
BBa_S03156
B0034.C0079
Jennifer Braff
2004-07-22T11:00:00Z
2015-05-08T01:14:20Z
false
true
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
false
component940989
1
BBa_C0079
component940976
1
BBa_B0034
annotation940989
1
BBa_C0079
range940989
1
19
774
annotation940976
1
BBa_B0034
range940976
1
1
12
BBa_I746320
1
BBa_I746320
GFP under control of PF promoter
Stefan Milde
2007-09-10T11:00:00Z
2015-08-31T04:08:04Z
false
false
_116_
0
2122
9
Released HQ 2013
In stock
false
This part is used to screen the level of induction of the PF promoter (I746360).
The PF promoter is induced by a class of phage activators (P2 ogr: I746350, PSP3pag: I746351, phiR73 delta: I746352).
The PF promoter was put upstream of I13504.
registry (I13504) and own construct (I746360)
false
component1943794
1
BBa_E0040
component1943792
1
BBa_B0034
component1943790
1
BBa_I746360
component1943797
1
BBa_B0012
component1943795
1
BBa_B0010
annotation1943792
1
BBa_B0034
range1943792
1
100
111
annotation1943790
1
BBa_I746360
range1943790
1
1
91
annotation1943794
1
BBa_E0040
range1943794
1
118
837
annotation1943795
1
BBa_B0010
range1943795
1
846
925
annotation1943797
1
BBa_B0012
range1943797
1
934
974
BBa_K775003
1
BBa_K775003
RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator
Sietske Grijseels
2012-09-04T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1027_
0
12035
9
Released HQ 2013
In stock
false
BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7.
-
N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
Ligand binding domain: C. elegans 7-transmembrane receptor ODR-10 U49449.1
C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
false
BBa_K823016
1
BBa_K823016
lacZ full-length (RBS+ TERM-)
Jara Radeck
2012-09-10T11:00:00Z
2015-05-08T01:13:30Z
false
false
_1081_
0
11555
9
Released HQ 2013
In stock
false
Full-length lacZ with an RBS ahead.
This is a copy of the [[Part:BBa_I732017|Part:BBa_I732017]]. Only here the part is cloned in the standard vector <b>pSB1C3</b> instead of the pSB1A2.
We changed the backbone vector of the part from pSB1A2 to <b>pSB1C3</b>
Part:BBa_I732017
false
component2182650
1
BBa_I732017
annotation2182650
1
BBa_I732017
range2182650
1
1
3093
BBa_K876057
1
BBa_K876057
pTetR-lasR-Term-Term
Leah Ferrell, Sanket Shah, Shayan Ghassemi, Zakaraya Ashour
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
false
_1138_
0
13835
9
Released HQ 2013
In stock
false
pTetR-lasR-TT
N/A
N/A
false
component2251293
1
BBa_B0015
component2251277
1
BBa_R0040
component2251286
1
BBa_C0079
annotation2251286
1
BBa_C0079
range2251286
1
61
841
annotation2251293
1
BBa_B0015
range2251293
1
850
978
annotation2251277
1
BBa_R0040
range2251277
1
1
54
BBa_K567015
1
BBa_K567015
PT7-metGN
You Wang
2011-09-28T11:00:00Z
2015-05-08T01:12:43Z
false
false
_735_
0
8782
9
Released HQ 2013
In stock
true
T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator. We have cloned metG from E.coli and the tRNA recognition domain of metG is truncated. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and metY-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LBKana plate.
overlapping PCR is used
metG from E.coli
false
annotation2150831
1
rbs
range2150831
1
75
81
annotation2153277
1
T7 TE
range2153277
1
1389
1436
annotation2153247
1
MetRS(truncated)
range2153247
1
89
1219
annotation2150830
1
lac operator
range2150830
1
21
44
annotation2150829
1
T7 promoter
range2150829
1
2
18
BBa_C0060
1
aiiA
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the autoinducer inactivation enzyme A (<em>aiiA</em>) LVA tagged. The gene was originally isolated from <em>Bacillus</em> sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.</P>
References (unparsed) here: <p>Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of <em>Erwinia<br> carotovora</em>, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). <a href="#">http://www.pnas.org/cgi/content/full/97/7/3526</a></P> <P>Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. <a href="#">http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491</a><br> <br> </P> <P> References (unparsed) here: <p>Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of <em>Erwinia<br> carotovora</em>, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). <a href="#">http://www.pnas.org/cgi/content/full/97/7/3526</a></P> <P>Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. <a href="#">http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491</a><br> <br> </P> <P>BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.<P>
<genbank>AF196486</genbank> from <em>Bacillus</em> sp. 240B1 putative metallohydrolase (<em>aiiA</em>) gene. <BR> Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of <em>Erwinia<br> carotovora</em>, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).<br>
true
annotation2213987
1
Help:Barcodes
range2213987
1
790
814
annotation7037
1
BBa_C0060
range7037
1
1
789
annotation1754
1
start
range1754
1
1
3
annotation1756
1
LVA
range1756
1
751
783
annotation1755
1
2
range1755
1
784
789
annotation1757
1
aiiA
range1757
1
1
750
BBa_S01022
1
BBa_S01022
B0034.E0020
Randy Rettberg
2005-06-15T11:00:00Z
2015-05-08T01:14:17Z
false
true
_1_
0
25
1
Released HQ 2013
In stock
false
Intermediate Part from Box 7
false
component1520219
1
BBa_E0020
component1520217
1
BBa_B0034
annotation1520219
1
BBa_E0020
range1520219
1
19
741
annotation1520217
1
BBa_B0034
range1520217
1
1
12
BBa_K731020
1
araC-CysE
Inducible araC-pBAD promoter regulating WT CysE
Jason Fontana
2012-08-20T11:00:00Z
2015-05-08T01:13:06Z
false
false
_977_
0
12113
9
Released HQ 2013
In stock
false
The CysE gene (K731000) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose.
CysE was inserted after araC-pBAD in his vector, cutting the former with X&P and the latter with S&P.
K731200, K731000
false
component2331169
1
BBa_K731201
component2331174
1
BBa_K731000
annotation2331174
1
BBa_K731000
range2331174
1
1212
2050
annotation2331169
1
BBa_K731201
range2331169
1
1
1203
BBa_S03119
1
BBa_S03119
--Specify Parts List--
Randy Rettberg
2004-04-19T11:00:00Z
2015-05-08T01:14:19Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Intermediate part from assembly 299
true
component955034
1
BBa_B0034
component955057
1
BBa_B0010
component955026
1
BBa_R0040
component955067
1
BBa_B0012
component955049
1
BBa_C0062
annotation955067
1
BBa_B0012
range955067
1
958
998
annotation955026
1
BBa_R0040
range955026
1
1
54
annotation955049
1
BBa_C0062
range955049
1
81
836
annotation955057
1
BBa_B0010
range955057
1
870
949
annotation955034
1
BBa_B0034
range955034
1
63
74
BBa_I6102
1
BBa_I6102
Promoter O_H Test R0073.E0430
Randy Rettberg
2004-04-21T11:00:00Z
2015-08-31T04:07:44Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component949769
1
BBa_B0012
component949752
1
BBa_B0034
component949747
1
BBa_R0073
component949754
1
BBa_E0030
component949759
1
BBa_B0010
annotation949759
1
BBa_B0010
range949759
1
825
904
annotation949754
1
BBa_E0030
range949754
1
94
816
annotation949747
1
BBa_R0073
range949747
1
1
67
annotation949752
1
BBa_B0034
range949752
1
76
87
annotation949769
1
BBa_B0012
range949769
1
913
953
BBa_E5501
1
BBa_E5501
B0032.E0040
jcbraff
2005-05-16T11:00:00Z
2015-08-31T04:07:27Z
false
true
_1_
0
61
1
Released HQ 2013
In stock
true
B0032.E0040
false
component1489101
1
BBa_E0040
component1489098
1
BBa_B0032
annotation1489098
1
BBa_B0032
range1489098
1
1
13
annotation1489101
1
BBa_E0040
range1489101
1
20
739
BBa_K936020
1
BBa_K936020
K206000+B0034+pelB+Cutinase+Polyhistidine Tag
Mattan Hamou
2012-09-19T11:00:00Z
2015-05-08T01:13:47Z
false
false
_1201_
0
13899
9
Released HQ 2013
In stock
false
Full Cutinase Construct on psB1A3 backbone.
There's no terminator so it needs to be used with a backbone that ends with a 3
http://www.google.com/url?q=http%3A%2F%2Faem.asm.org%2Fcontent%2F78%2F5%2F1556.long&sa=D&sntz=1&usg=AFQjCNGjFC2TH89mPrYkWCAiYVccsHzdig
false
component2188714
1
BBa_K936014
component2188710
1
BBa_K936015
annotation2188710
1
BBa_K936015
range2188710
1
1
150
annotation2188714
1
BBa_K936014
range2188714
1
157
1125
BBa_K775002
1
BBa_K775002
RI7-OR1G1 Isoamylacetate chimeric GPCR
Sietske Grijseels
2012-09-04T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1027_
0
12035
9
Released HQ 2013
In stock
false
BBa_K775002 encodes a chimeric isoamylacetate-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mouse OR1G1, flanked by the N- and C- terminals of the rat OR RI7.
-
N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
Ligand binding domain: ORG1, (Homo sapiens) NM_003555
C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
false
BBa_K737032
1
BBa_K737032
Spot42 based small RNA2
Peiran Zhang and Minghao Gong
2012-09-17T11:00:00Z
2015-05-08T01:13:07Z
false
false
_986_
0
14291
9
Released HQ 2013
In stock
false
Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose operon and other sugar metabolic pathway,such as galK,nanC,ytfJ,srlA,which are the 5??? leader sequence of the corresponding metabolic enzymes.
Take galK for example,Spot42 causes translation repression by base pairing to RBS in the 5??? leader sequence,then block the recognition of the antiSD sequence on 30S subunit.
It???s a very strong pairing(up to 20bases),but it only causes 2.6 fold repression according to Johannes H. Urban and Jo?? rg Vogel,for it doesn???t result in the degradation of the RNA complex by RNaseE(ssRNA degradation) and RNaseIII(dsRNA degradation),both of them are major enzymes that causes RNA degradation in vivo.Spot42-galK complex degrades in a slow and presently unclear way. The base pairing is initiated by the recognition of seed region,which plays a significant role in the repression efficiency.
No
No
false
BBa_J202002
1
BBa_J202002
PBAD Mutant#3
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -71 relative to the araC transcriptional start site was changed from A to G. Also, C at -22 was changed to T
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by random mutagenesis.
false
annotation2178422
1
araI2
range2178422
1
61
77
annotation2178425
1
Mutation 2
range2178425
1
90
90
annotation2178421
1
araI1
range2178421
1
40
56
annotation2178424
1
Mutation 1
range2178424
1
41
41
annotation2178423
1
Transcription Start
range2178423
1
112
112
BBa_K863204
1
BBa_K863204
shuttle vector pECPP11JS for recombination of genes of interest in yeast
Julia Schirmacher
2012-09-22T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
13076
9
Released HQ 2013
In stock
true
long description is coming soon
the design considerations are coming soon
This part is made of the BioBricks XYZ by gibson assembly.
false
annotation2200007
1
3' AOX
range2200007
1
4184
4867
annotation2200005
1
AOX1 terminator
range2200005
1
1250
1531
annotation2200002
1
5' AOX
range2200002
1
1
940
annotation2200003
1
Kosak sequence
range2200003
1
941
952
annotation2200006
1
his4
range2200006
1
1615
4143
annotation2200004
1
MFalpha
range2200004
1
953
1215
BBa_K784013
1
BBa_K784013
pLux+Theophylline riboswitch+mCherry
Ilya Vainberg Slutskin
2012-09-15T11:00:00Z
2015-05-08T01:13:21Z
false
true
_1039_
0
11677
9
Released HQ 2013
In stock
false
This sequence is the product of restriction cloning of the [[Part:BBa_K784006|theophylline riboswitch]] downstream to the [[Part:BBa_R0062|pLux promoter]]. In the presence of [[Part:BBa_I0462|LuxR]] transcription can be induced by [[3OC6HSL|3OC<sub>6</sub>HSL]].
No design considerations.
Cloning of [[Part:BBa_K784006|theophylline riboswitch]] downstream to the [[Part:BBa_R0062|pLux promoter]]
false
component2183694
1
BBa_K784006
component2183682
1
BBa_R0062
annotation2183694
1
BBa_K784006
range2183694
1
64
851
annotation2183682
1
BBa_R0062
range2183682
1
1
55
BBa_I716208
1
BBa_I716208
[lox][terminator][lox]
Samantha Liang
2007-06-04T11:00:00Z
2015-08-31T04:07:51Z
false
false
_110_
0
933
52
Released HQ 2013
In stock
false
[lox][terminator][lox]
Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large)
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small)
Product is pBca9145-I716208.
Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large)
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small)
Product is pBca9145-I716208.
false
BBa_I732810
1
BBa_I732810
ARL2A0203 (RBS+, TERM-)
Zhan Jian
2007-10-13T11:00:00Z
2015-08-31T04:07:59Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1946192
1
BBa_B0034
component1946193
1
BBa_I732110
annotation1946192
1
BBa_B0034
range1946192
1
1
12
annotation1946193
1
BBa_I732110
range1946193
1
19
1104
BBa_I732904
1
BBa_I732904
R0040 B0015
Zhan Jian
2007-10-16T11:00:00Z
2015-08-31T04:08:00Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1948594
1
BBa_B0012
component1948588
1
BBa_R0040
component1948592
1
BBa_B0010
annotation1948592
1
BBa_B0010
range1948592
1
63
142
annotation1948588
1
BBa_R0040
range1948588
1
1
54
annotation1948594
1
BBa_B0012
range1948594
1
151
191
BBa_K737051
1
BBa_K737051
IPTG inducible GFP Generator
Weihong Lai and Peiran Zhang
2012-09-18T11:00:00Z
2015-05-08T01:13:08Z
false
false
_986_
0
14291
9
Released HQ 2013
In stock
false
[J23106,P0412]is a constitutive lacI repressor generator,Fluorescence can be observed with induced by IPTG.
The RBS of E0240 is B0030,a weak GFP translational unit specialized to quantitively describe the behaviour of promotor in many cases.
NOTE: R0011 don???t work well in high copy number plasmids.
No
No
false
component2185914
1
BBa_E0240
component2185899
1
BBa_R0011
component2185885
1
BBa_J23106
component2185898
1
BBa_P0412
annotation2185885
1
BBa_J23106
range2185885
1
1
35
annotation2185898
1
BBa_P0412
range2185898
1
44
1351
annotation2185899
1
BBa_R0011
range2185899
1
1360
1413
annotation2185914
1
BBa_E0240
range2185914
1
1423
2298
BBa_K778001
1
fhlA
fhlA: a transcriptional activator for enhanced hydrogen production
Tomohito Minakuchi
2012-09-21T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1032_
0
13134
9
Released HQ 2013
In stock
true
fhlA
pst1
Escherichia coli (JM109)
false
annotation2201429
1
FhlA protein
range2201429
1
1
2079
annotation2200693
1
A to G (1906bp) (different from E. coli K-12 MG1655)
range2200693
1
1906
1906
annotation2200692
1
Illegal Pst1_site deletion (1854bp)
range2200692
1
1854
1854
annotation2200694
1
stop
range2200694
1
2077
2079
annotation2200691
1
start
range2200691
1
1
1
BBa_K844007
1
BBa_K844007
Spider Silk 1x Subunit "F" (Fewest tRNA codon optimized) with Met (ATG) added
Charles Barentine
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
false
false
_1104_
0
10131
9
Released HQ 2013
In stock
false
Spider silk subunit optimized to use the fewest number of tRNA codons; contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.
none
Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844003
false
annotation2212476
1
Beta-spiral
range2212476
1
117
132
annotation2212479
1
Linker Domain
range2212479
1
172
188
annotation2212475
1
Beta-spiral
range2212475
1
98
113
annotation2212473
1
Elasticity Domain
range2212473
1
88
171
annotation2212474
1
Beta-helix
range2212474
1
88
97
annotation2212466
1
Start
range2212466
1
1
3
annotation2212468
1
Beta-helix
range2212468
1
4
12
annotation2212478
1
Beta-spiral
range2212478
1
156
171
annotation2212470
1
Beta-spiral
range2212470
1
34
48
annotation2212480
1
Strength Domain
range2212480
1
189
207
annotation2212471
1
Beta-spiral
range2212471
1
49
63
annotation2212467
1
Elasticity Domain
range2212467
1
4
87
annotation2212472
1
Beta-spiral
range2212472
1
72
87
annotation2212477
1
Beta-spiral
range2212477
1
133
147
annotation2212469
1
Beta-spiral
range2212469
1
13
27
BBa_K861042
1
BBa_K861042
FadA, gene for fatty acid degradation from <i>Salmonella enterica</i> LT2
Kuanwei Sheng
2012-09-05T11:00:00Z
2015-05-08T01:13:35Z
false
false
_1121_
0
12357
9
Released HQ 2013
In stock
false
FadA gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadA have far better efficiency compared to FadA in E.coli.(???S-??? is used to distinguish FadA gene in E.coli )
None
E.coli str.K12 lab mutant DH5α
false
BBa_J202006
1
BBa_J202006
PBAD Mutant#7
Jiyeon Park
2012-07-25T11:00:00Z
2015-08-31T04:08:37Z
false
false
_910_
0
11164
9
Released HQ 2013
In stock
false
This is PBAD (BBa_I13453) mutant. -66 relative to the araC transcriptional start site was changed from T to G.
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
This mutant was created by site-directed mutagenesis.
false
annotation2178443
1
Mutation
range2178443
1
46
46
annotation2178442
1
araI2
range2178442
1
61
77
annotation2178441
1
araI1
range2178441
1
40
56
annotation2178440
1
Transcription Start
range2178440
1
112
112
BBa_C0261
1
BBa_C0261
AHL-making Enzyme, luxI (+RBS)
robertb
2004-08-22T11:00:00Z
2015-08-31T04:07:24Z
false
false
_6_
0
159
7
Released HQ 2013
In stock
true
B0034.C0061
true
component1060413
1
BBa_C0061
component1060403
1
BBa_B0034
annotation1060413
1
BBa_C0061
range1060413
1
19
636
annotation1060403
1
BBa_B0034
range1060403
1
1
12
BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation1714
1
RBS
range1714
1
7
10
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
annotation7028
1
BBa_B0033
range7028
1
1
11
BBa_C0051
1
cI lam
cI repressor from E. coli phage lambda (+LVA)
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).</P>
References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P> References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P>BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.<P>
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
true
annotation23334
1
cI lambda
range23334
1
4
711
annotation23335
1
LVA
range23335
1
712
744
annotation2213991
1
Help:Barcodes
range2213991
1
751
775
BBa_K911003
1
BBa_K911003
Fluoride Sensitive Riboswitch
Oliver Meacock
2012-09-11T11:00:00Z
2015-05-08T01:13:45Z
false
false
_1176_
0
12694
9
Released HQ 2013
In stock
true
Riboswitch that is highly sensitive to the F- ion. We have characterized this part in three different chassis: TOP10 e.coli, Laboratory strain bacillus subtilis and a strain of bacillus subtilis with its normal fluoride riboswitch knocked out (kindly provided by the Breaker lab in Yale). Results of Miller assays for these three chassis are shown below.
Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter, in this case lacZ as our reporter.
Bacillus Cereus Genome
false
annotation2182732
1
Lysine promoter
range2182732
1
1
43
annotation2182733
1
Fluoride riboswitch
range2182733
1
61
139
BBa_I716210
1
BBa_I716210
BamHI endonuclease without start codon
Samantha Liang
2007-06-04T11:00:00Z
2015-08-31T04:07:51Z
false
false
_110_
0
933
52
Released HQ 2013
In stock
false
BamHI endonuclease without start codon
PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L)
Product is pBca9145-I716210
sil04F Forward BglII biobrick of BamHI
ccaaaAGATCTgaagtagaaaaagagtttattactga
sil04R Reverse XholI/BamHI biobrick of BamHI
gctagCTCGAGttaGGATCCctatttgttttcaactttatc
PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L)
Product is pBca9145-I716210
false
BBa_J61051
1
BBa_J61051
[Psal1]
John Anderson
2007-02-20T12:00:00Z
2015-08-31T02:03:00Z
false
false
_95_
0
483
95
Released HQ 2013
In stock
false
Salicylate promoter and nahR
nonh
non
false
BBa_E0026
1
ECFP
enhanced cyan fluorescent protein derived from A. victoria GFP
jcbraff
2004-06-06T11:00:00Z
2015-08-31T04:07:25Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
-- No description --
true
BBa_J01060
1
[pTET][loc
OnLock1 = [pTet][Lock1]
Golden Bear
2005-11-05T12:00:00Z
2015-08-31T04:08:12Z
false
true
_13_
0
395
13
Released HQ 2013
In stock
false
[pTet][Lock1]
false
component1738591
1
BBa_R0040
component1738596
1
BBa_J01010
annotation1738591
1
BBa_R0040
range1738591
1
1
54
annotation1738596
1
BBa_J01010
range1738596
1
63
102
BBa_S03518
1
BBa_S03518
B0034:C0040
Azeem Kaka
2006-07-23T11:00:00Z
2015-05-08T01:14:24Z
false
false
_58_
0
1061
58
Released HQ 2013
In stock
true
true
component2243681
1
BBa_B0034
component2243685
1
BBa_C0040
annotation2243681
1
BBa_B0034
range2243681
1
1
12
annotation2243685
1
BBa_C0040
range2243685
1
19
703
BBa_J01141
1
BBa_J01141
[pLacI pL][key1]
Golden Bear (Berkeley iGEM 2005)
2006-04-13T11:00:00Z
2015-08-31T04:08:13Z
false
false
_13_1_
0
612
13
Released HQ 2013
In stock
false
-- No description --
false
component1843688
1
BBa_J01008
component1843682
1
BBa_R0011
annotation1843682
1
BBa_R0011
range1843682
1
1
54
annotation1843688
1
BBa_J01008
range1843688
1
64
157
BBa_I13453
1
BBa_I13453
Pbad promoter
jkm
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
false
false
_11_
0
253
6
Released HQ 2013
In stock
false
PBad promoter from I0500 without AraC.
true
BBa_J61001
1
R6K
[R6K] Origin of replication
John Anderson
2006-07-30T11:00:00Z
2015-08-31T02:02:59Z
false
false
_95_
0
483
95
Released HQ 2013
In stock
false
R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature.
{pSB1A2-Bca1011}
N/A
<tt>
PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI)<br>
Sub into pSB1A2-I13522 (EcoRI/SpeI)<br>
Product is Bca1011<br>
----
ca1011F Forward Biobricking of R6K<br>
GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg<br>
ca1011R Reverse biobricking of R6K<br>
ccgctactagtaGCAGTTCAACCTGTTGATAG<br>
</tt>
true
annotation1892271
1
R6K
range1892271
1
27
406
BBa_K902059
1
BBa_K902059
katG +LAA behind an RBS (B0034)
Lisa Oberding, Ali Honarmand
2012-09-30T11:00:00Z
2015-05-08T01:13:43Z
false
false
_1167_
0
13399
9
Released HQ 2013
In stock
false
KatG +LAA behind and RBS site (B0034)
None
Registry
false
component2205168
1
BBa_B0034
component2205170
1
BBa_K137068
annotation2205170
1
BBa_K137068
range2205170
1
19
2235
annotation2205168
1
BBa_B0034
range2205168
1
1
12
BBa_I6060
1
BBa_I6060
Promoter O_H Test R0010.E0432
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948916
1
BBa_B0010
component948926
1
BBa_B0012
component948886
1
BBa_R0010
component948908
1
BBa_E0032
component948896
1
BBa_B0034
annotation948916
1
BBa_B0010
range948916
1
997
1076
annotation948908
1
BBa_E0032
range948908
1
227
988
annotation948926
1
BBa_B0012
range948926
1
1085
1125
annotation948886
1
BBa_R0010
range948886
1
1
200
annotation948896
1
BBa_B0034
range948896
1
209
220
BBa_K737031
1
BBa_K737031
Spot42 based small RNA1
Peiran Zhang and Minghao Gong
2012-09-17T11:00:00Z
2015-05-08T01:13:07Z
false
false
_986_
0
14291
9
Released HQ 2013
In stock
false
Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose operon and other sugar metabolic pathway,such as galK,nanC,ytfJ,srlA,which are the 5??? leader sequence of the corresponding metabolic enzymes.
Take galK for example,Spot42 causes translation repression by base pairing to RBS in the 5??? leader sequence,then block the recognition of the antiSD sequence on 30S subunit.
It???s a very strong pairing(up to 20bases),but it only causes 2.6 fold repression according to Johannes H. Urban and Jo?? rg Vogel,for it doesn???t result in the degradation of the RNA complex by RNaseE(ssRNA degradation) and RNaseIII(dsRNA degradation),both of them are major enzymes that causes RNA degradation in vivo.Spot42-galK complex degrades in a slow and presently unclear way. The base pairing is initiated by the recognition of seed region,which plays a significant role in the repression efficiency.
No
No
false
BBa_K849000
1
HMG-CoA-R
3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast
Leonard Sebastian Fresenborg
2012-09-19T11:00:00Z
2015-05-08T01:13:34Z
false
false
_1109_
0
12733
9
Released HQ 2013
In stock
true
This is a truncated version of the 3-Hydroxy-3-Methyl-Glutaryl-Coenzyme-A-Reductase (HMG-CoA-R) from ''Saccharomyces cerevisiae''. It catalyses the reaction of 3-Hydroxy-3-Methyl-Glutaryle-Coenzyme-A to Mevalonate which occurs in the Mevalonate Pathway to Isopentylpyrophosphate which is the Precurser of all Isoprenoides. The enzyme is naturally a membrane bound enzyme. The membrane anchor is situated N-terminal. This region is also essential for the degradational control of the enzyme. By deleting the N-terminal part to Aminoacid (inclusive) the enzyme is expected to be water soluble and deregulated.
The truncation was done according to the following publication: <br>
[[http://aem.asm.org/content/76/19/6449.short|Rico, Pardo, Orejas: 'Enhanced Production of a Plant Monoterpene by Overexpression of the 3-Hydroxy-3-Methylglutaryl Conenzyme A Reductase Catalytic Domain in Saccharomyces cerevisiae'; Applied and Enviromental Microbiology; Vol 76 p. 6449-6454; 2010]]
The original source of this part is the genome of Saccharomyces cerevisiae. We obtained the sequence by de novo synthesis. For that we used data from the [[http://www.yeastgenome.org/|Saccharomyes Genome Database]]. We inserted some calm mutations to avoid conflicts with the Cloning Standard 10.
false
annotation2202427
1
cds
range2202427
1
1
1584
BBa_R0077
1
cinR
Promoter (cinR and HSL regulated, RBS+)
crackdots
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription.
This promoter contains the RBS: TGGAGG
The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci
Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ
MOL. MICROBIOLOGY 37(1): 81-97, 2000
Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
Rhizobium leguminosarum
true
annotation301205
1
stem_loop
range301205
1
91
136
annotation301371
1
unidentified/uncharacterized CinR dependent
range301371
1
1
220
annotation301150
1
embedded RBS
range301150
1
226
231
annotation301204
1
stem_loop
range301204
1
35
74
BBa_K805011
1
Cel5A
Cel5A, endoglucanases
Liu Ping
2012-09-19T11:00:00Z
2015-06-19T01:48:30Z
false
false
_1062_
4206
11895
9
Released HQ 2013
In stock
true
test
test
yes
false
annotation2200608
1
protein
range2200608
1
4
1233
annotation2200607
1
start codon
range2200607
1
1
3
BBa_I13602
1
BBa_I13602
Tet operator with CFP reporter (with LVA tag) [R/Tc+]
Christopher Batten, Victoria Chou, Kenneth Nesmith
2004-07-15T11:00:00Z
2015-08-31T04:07:36Z
false
true
_6_
0
101
7
Released HQ 2013
In stock
false
-- No description --
true
component947292
1
BBa_B0012
component947254
1
BBa_R0040
component947262
1
BBa_B0034
component947282
1
BBa_B0010
component947274
1
BBa_E0022
annotation947274
1
BBa_E0022
range947274
1
81
842
annotation947292
1
BBa_B0012
range947292
1
939
979
annotation947262
1
BBa_B0034
range947262
1
63
74
annotation947254
1
BBa_R0040
range947254
1
1
54
annotation947282
1
BBa_B0010
range947282
1
851
930
BBa_K929000
1
CMV AID pA
AID with CMV promoter and hGH-polyadenylation signal sequence
Potsdam Bioware 2012 iGEM
2012-09-15T11:00:00Z
2015-05-08T01:13:46Z
false
false
_1194_
0
14457
9
Released HQ 2013
In stock
false
The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K103001). It is built of 3 parts: CMV promoter, AID and hGH-polyA tail.
AID:
AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes.
CMV promoter:
CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter.
hGH-polyA tail:
The polyA tail consists of multiple adenosine monophosphates. hGH-polyA tail is a significant part for the translation and stability of mRNA. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.
bla bla
It is a composite part consisting of 3 other Biobricks.
false
annotation2197703
1
BBa_K404108
range2197703
1
1276
1754
annotation2197697
1
NcoI
range2197697
1
663
668
annotation2197700
1
AUG
range2197700
1
665
667
annotation2197695
1
CMV promoter
range2197695
1
1
654
annotation2197698
1
BBa_K103001
range2197698
1
663
1262
annotation2197696
1
BBa_I712004
range2197696
1
1
654
annotation2197702
1
hGH terminator
range2197702
1
1276
1754
annotation2197699
1
AID
range2197699
1
665
1262
annotation2197701
1
UGA
range2197701
1
1259
1262
BBa_R1062
1
lux pR
Promoter, Standard (luxR and HSL regulated -- lux pR)<br>
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr<br> Drew Endy<br>
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
[Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].</p> <p>Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"><br><br> Modified to comply with BBa_S0001:<br> TTGACA-17N-GATACT<br> <P>
<em>V. fischeri</em>
true
annotation2100
1
start
range2100
1
54
54
annotation7077
1
BBa_R1062
range7077
1
1
56
annotation2101
1
-35
range2101
1
20
25
annotation2099
1
-10
range2099
1
42
48
annotation2098
1
LuxR/HSL
range2098
1
1
20
BBa_S03154
1
BBa_S03154
B0034.C0078
Jennifer Braff
2004-07-22T11:00:00Z
2015-05-08T01:14:20Z
false
false
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
true
component940972
1
BBa_C0078
component940962
1
BBa_B0034
annotation940962
1
BBa_B0034
range940962
1
1
12
annotation940972
1
BBa_C0078
range940972
1
19
660
BBa_S01002
1
BBa_S01002
R0071.E0422
2005-06-10T11:00:00Z
2015-05-08T01:14:16Z
false
false
_1_
0
25
1
Released HQ 2013
In stock
false
Intermediate part from Jen 1 Tube 46
false
component1511043
1
BBa_E0022
component1511026
1
BBa_R0071
component1511031
1
BBa_B0034
component1511061
1
BBa_B0012
component1511051
1
BBa_B0010
annotation1511031
1
BBa_B0034
range1511031
1
62
73
annotation1511026
1
BBa_R0071
range1511026
1
1
53
annotation1511043
1
BBa_E0022
range1511043
1
80
841
annotation1511061
1
BBa_B0012
range1511061
1
938
978
annotation1511051
1
BBa_B0010
range1511051
1
850
929
BBa_I6108
1
BBa_I6108
Promoter O_H Test R0080.E0430
cconboy
2004-05-09T11:00:00Z
2015-08-31T04:07:44Z
false
false
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
false
component949969
1
BBa_B0010
component949957
1
BBa_R0080
component949979
1
BBa_B0012
component949962
1
BBa_B0034
component949964
1
BBa_E0030
annotation949979
1
BBa_B0012
range949979
1
995
1035
annotation949969
1
BBa_B0010
range949969
1
907
986
annotation949957
1
BBa_R0080
range949957
1
1
149
annotation949964
1
BBa_E0030
range949964
1
176
898
annotation949962
1
BBa_B0034
range949962
1
158
169
BBa_C0061
1
luxI
autoinducer synthetase for AHL
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Synthesizes 3OC<sub>6</sub>HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
<P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P>
<em>V. fischeri</em> <genbank>AF170104</genbank>
true
annotation1760
1
LVA
range1760
1
580
611
annotation2213985
1
Help:Barcodes
range2213985
1
619
643
annotation7038
1
BBa_C0061
range7038
1
1
618
annotation1761
1
luxI
range1761
1
1
579
BBa_K880002
1
BBa_K880002
GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.
Josh Atkinson, Mike Ferguson, and Ben Parker
2012-09-28T11:00:00Z
2015-05-08T01:13:40Z
false
false
_1142_
0
9403
9
Released HQ 2013
In stock
false
-IRR K137008 + inverted tetR promoter K137047 + IRL K137010 + GFP I13504 (MI275)
-GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases. Same components as K137058 with the inverted repeats reversed such that the external half sites are external to the flip region (IRR - ???flip region??? - IRL)
GFP fluorescent reporter capable of assaying the functionality of fim regulatory recombinases; based on K137058.
K137058???s invertible repeat sequences responsible for recombinase binding are ordered incorrectly; the regions are not identical, and have ???left??? and ???right??? components. K137008 (invertible repeat-right) and K137010 (invertible repeat-left) are mislabeled and belong in the order ???K137008-region to invert-K137010??? in accordance with the wild-type fim system.
Repeat sequence in this part exist in the correct (wild-type) orientation.
Enter design considerations
Enter source
false
component2204543
1
BBa_K137008
component2204545
1
BBa_K137047
component2204548
1
BBa_K137010
component2204558
1
BBa_I13504
annotation2204548
1
BBa_K137010
range2204548
1
302
336
annotation2204545
1
BBa_K137047
range2204545
1
44
293
annotation2204558
1
BBa_I13504
range2204558
1
345
1219
annotation2204543
1
BBa_K137008
range2204543
1
1
35
BBa_I13202
1
BBa_I13202
3OC<sub>6</sub>HSL Sender Controlled by Lac Repressible Promoter
Barry Canton
2004-07-29T11:00:00Z
2015-08-31T04:07:33Z
false
true
_101_6_
0
135
7
Released HQ 2013
In stock
false
A lac repressible promoter driving production of an untagged LuxI enzyme which produces an AHL (3OC<sub>6</sub>HSL).
false
component1484926
1
BBa_R0011
component1484935
1
BBa_B0034
component1484942
1
BBa_B0010
component1484952
1
BBa_B0012
component1484937
1
BBa_C0161
annotation1484942
1
BBa_B0010
range1484942
1
675
754
annotation1484952
1
BBa_B0012
range1484952
1
763
803
annotation1484937
1
BBa_C0161
range1484937
1
82
666
annotation1484926
1
BBa_R0011
range1484926
1
1
54
annotation1484935
1
BBa_B0034
range1484935
1
64
75
BBa_E0430
1
BBa_E0430
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
Caitlin Conboy
2004-03-15T12:00:00Z
2015-08-31T04:07:26Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Standard YFP Output Device -LVA tag
true
component942892
1
BBa_B0010
component942885
1
BBa_B0034
component942887
1
BBa_E0030
component942902
1
BBa_B0012
annotation942902
1
BBa_B0012
range942902
1
838
878
annotation942892
1
BBa_B0010
range942892
1
750
829
annotation942885
1
BBa_B0034
range942885
1
1
12
annotation942887
1
BBa_E0030
range942887
1
19
741
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
represillator of Elowitz and Leibler (2000)
true
annotation2001
1
lac O1
range2001
1
26
42
annotation1999
1
lac O1
range1999
1
3
19
annotation2002
1
-10
range2002
1
43
48
annotation2000
1
-35
range2000
1
20
25
annotation7064
1
BBa_R0011
range7064
1
1
54
BBa_K737004
1
BBa_K737004
Inducible GVPA Generator
Li Kang
2012-09-17T11:00:00Z
2015-05-08T01:13:07Z
false
false
_986_
0
14291
9
Released HQ 2013
In stock
false
This part contains a constitutive promoter (J23106), a coding region of LacI protein with an LVA degradation tail, a terminator (B0015), a promoter repressed by LacI protein (R0011) a coding region of GvpA and GFP and a terminator (B0015) behind it.
This part can be used to induce the strength of promoter (R0011) by IPTG and indicate the content of GvpA by fluorescence of GFP. In short, it can regulate the expression of GvpA.
We can use different concentration of IPTG to regulate the strength of promoter (R0011) ahead GvpA Protein and GFP. And We can know the approximate concentration of GvpA by the fluorescence unit of GFP.
To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP. Here is the curve:
We can use different concentration of IPTG to regulate the strength of promoter (R0011) ahead GvpA Protein and GFP. And We can know the approximate concentration of GvpA by the fluorescence unit of GFP.
To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.
No
false
component2184827
1
BBa_B0034
component2184828
1
BBa_K737016
component2184807
1
BBa_J23106
component2184821
1
BBa_R0011
component2184839
1
BBa_E0840
component2184820
1
BBa_P0412
annotation2184827
1
BBa_B0034
range2184827
1
1423
1434
annotation2184807
1
BBa_J23106
range2184807
1
1
35
annotation2184820
1
BBa_P0412
range2184820
1
44
1351
annotation2184828
1
BBa_K737016
range2184828
1
1441
1659
annotation2184821
1
BBa_R0011
range2184821
1
1360
1413
annotation2184839
1
BBa_E0840
range2184839
1
1668
2545
BBa_K880005
1
BBa_K880005
Strong promoter, strong RBS combination for high expression levels of proteins
Josh Atkinson, Mike Ferguson, and Ben Parker
2012-09-28T11:00:00Z
2015-05-08T01:13:40Z
false
false
_1142_
0
9403
9
Released HQ 2013
In stock
false
-J23100+B0034
-Strong promoter, strong RBS combination for high expression levels of proteins
Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
Enter design considerations.
It is a combination of strong promoter (J23100) and RBS (B0034).
false
component2204228
1
BBa_J23100
component2204230
1
BBa_B0034
annotation2204230
1
BBa_B0034
range2204230
1
44
55
annotation2204228
1
BBa_J23100
range2204228
1
1
35
BBa_C0160
1
aiiA
autoinducer inactivation enzyme aiiA (no LVA)
jcbraff
2004-05-26T11:00:00Z
2015-08-31T04:07:24Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
same as C0060 except no LVA tag
true
annotation1891585
1
aiiA
range1891585
1
1
756
BBa_K731250
1
BBa_K731250
Inducible araC-pBAD promoter with GFPmut3b, strong RBS
Daniele Rossetto
2012-08-02T11:00:00Z
2015-05-08T01:13:06Z
false
false
_977_
0
11997
9
Released HQ 2013
In stock
true
This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200).
The part is composed of part BBa_K731200 and BBa_E0840
araC-pBAD from E. coli genome, GFPmut3b is from the registry (part BBa_E0840)
false
component2331165
1
BBa_E0240
component2331154
1
BBa_K731201
annotation2331165
1
BBa_E0240
range2331165
1
1212
2087
annotation2331154
1
BBa_K731201
range2331154
1
1
1203
BBa_K748003
1
BBa_K748003
yddV is a Diguanylate Cyclase-Genomic.
Lei Qiao
2012-09-14T11:00:00Z
2015-05-08T01:13:12Z
false
false
_999_
0
8564
9
Released HQ 2013
In stock
false
yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
nothing special
de novo synthesis
false
BBa_E5504
1
BBa_E5504
R0040.B0032.E0040
jcbraff
2005-05-16T11:00:00Z
2015-08-31T04:07:27Z
false
false
_1_
0
61
1
Released HQ 2013
In stock
false
R0040.B0032.E0040 (R0040.E5501)
false
component1489160
1
BBa_R0040
component1489174
1
BBa_E0040
component1489171
1
BBa_B0032
annotation1489174
1
BBa_E0040
range1489174
1
82
801
annotation1489160
1
BBa_R0040
range1489160
1
1
54
annotation1489171
1
BBa_B0032
range1489171
1
63
75
BBa_K876067
1
BBa_K876067
Term-Term-pLac-LuxR
Zakaraya Ashour
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
true
_1138_
0
13835
9
Released HQ 2013
In stock
false
TT-pLac-LuxR
N/A
N/A
false
component2251300
1
BBa_B0015
component2251310
1
BBa_C0062
component2251301
1
BBa_R0010
annotation2251310
1
BBa_C0062
range2251310
1
344
1099
annotation2251301
1
BBa_R0010
range2251301
1
138
337
annotation2251300
1
BBa_B0015
range2251300
1
1
129
BBa_K773004
1
BBa_K773004
mCherry-AAV
Katie Knister
2012-09-30T11:00:00Z
2015-05-08T01:13:15Z
false
false
_1025_
0
11646
9
Released HQ 2013
In stock
false
This part holds the fluorescent protein mCherry attached to a degradation tag AAV which breaks down fluorescent protein over time.
We eliminated a restriction site from the gene.
This part was modified from a plasmid provided by the Murray lab at Caltech.
false
annotation2209503
1
mCherry-AAV
range2209503
1
19
765
annotation2209506
1
ssrA degradation tag: AAV
range2209506
1
730
759
annotation2209504
1
Stop
range2209504
1
760
762
annotation2209502
1
B0034
range2209502
1
1
12
annotation2209507
1
Start
range2209507
1
19
21
annotation2209505
1
Stop
range2209505
1
763
765
BBa_I732007
1
Adapter
BglII + BamHI
Zhan Jian
2007-07-06T11:00:00Z
2015-08-31T04:07:56Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
BglII and BamHI restriction sites with some flanking
None
[[http://openwetware.org/wiki/Silver:_Oligonucleotide_Inserts Oligonucleotide Inserts]]
true
annotation1936908
1
BamHI
range1936908
1
12
17
annotation1936907
1
BglII
range1936907
1
3
8
BBa_J01003
1
OriTR
OriT-R (Origin of transfer for the R-plasmid nic region)
Golden Bear
2005-10-17T11:00:00Z
2015-08-31T04:08:11Z
false
false
_13_
0
395
13
Released HQ 2013
In stock
false
OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.
true
BBa_I6064
1
BBa_I6064
Promoter O_H Test R0050.E0432
Caitlin Conboy and Jennifer Braff
2004-03-17T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component949072
1
BBa_B0012
component949054
1
BBa_E0032
component949062
1
BBa_B0010
component949032
1
BBa_R0050
component949042
1
BBa_B0034
annotation949062
1
BBa_B0010
range949062
1
852
931
annotation949042
1
BBa_B0034
range949042
1
64
75
annotation949054
1
BBa_E0032
range949054
1
82
843
annotation949072
1
BBa_B0012
range949072
1
940
980
annotation949032
1
BBa_R0050
range949032
1
1
55
BBa_K801072
1
BBa_K801072
3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep
Roman Prechtl
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
false
false
_1057_
0
11966
9
Released HQ 2013
In stock
false
3,7-dimethylxanthine N-methyltransferase CaDXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
-
http://www.ncbi.nlm.nih.gov/nuccore/AB084125
synthesized by IDT, yeast codon optimized
false
annotation2198187
1
Strep Tag
range2198187
1
1159
1188
annotation2197893
1
Consensus sequence
range2197893
1
1
6
annotation2197894
1
CaDXMT1
range2197894
1
7
1158
annotation2198588
1
Stop codon
range2198588
1
1189
1194
annotation2198600
1
Start codon
range2198600
1
7
9
BBa_K750010
1
TD0.6
TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP
Sifan Wang, Ruosang Qiu, Shuqin Hu,Zhao Ma,Yunxin Long
2012-09-18T11:00:00Z
2015-05-08T01:13:12Z
false
false
_1001_
0
14212
9
Released HQ 2013
In stock
false
This part contains part5 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
editing...
We built this part by biobricks from the DNA distribution kit plates 2012.
false
component2312702
1
BBa_K750007
component2312665
1
BBa_K750002
annotation2312702
1
BBa_K750007
range2312702
1
948
2967
annotation2312665
1
BBa_K750002
range2312665
1
1
939
BBa_R2109
1
BBa_R2109
Promoter with operator site for C2003
Reshma Shetty
2006-10-20T11:00:00Z
2015-05-08T01:14:16Z
false
false
_41_
0
126
70
Released HQ 2013
In stock
false
This promoter is designed to bind to C2003.
Placement of the operator site was determined to optimize steric interference between C2003 and RNA polymerase.
This promoter was rationally designed and synthesized.
false
annotation1903913
1
-10 site
range1903913
1
40
45
annotation1903914
1
transcription start site
range1903914
1
53
53
annotation1903912
1
-35 site
range1903912
1
17
22
annotation1903915
1
operator site for C2003
range1903915
1
22
33
BBa_I13027
1
BBa_I13027
penI QPI intermediate
jenmitch
2004-07-14T11:00:00Z
2015-08-31T04:07:32Z
false
true
_6_
0
159
7
Released HQ 2013
In stock
false
-- No description --
false
component939448
1
BBa_B0034
component939458
1
BBa_C0074
annotation939448
1
BBa_B0034
range939448
1
1
12
annotation939458
1
BBa_C0074
range939458
1
19
441
BBa_J24679
1
BBa_J24679
RBS + LacI
Annie Gao
2006-10-25T11:00:00Z
2015-08-31T04:08:41Z
false
false
_58_
0
1017
58
Released HQ 2013
In stock
true
Adds an RBS to LacI protein coding region
Strength of RBS
composite of other iGEM parts
true
component2243741
1
BBa_B0033
component2243744
1
BBa_C0012
annotation2243744
1
BBa_C0012
range2243744
1
18
1145
annotation2243741
1
BBa_B0033
range2243741
1
1
11
BBa_I6046
1
BBa_I6046
Promoter O_H Test R0065.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948627
1
BBa_B0010
component948608
1
BBa_R0065
component948637
1
BBa_B0012
component948620
1
BBa_B0034
component948622
1
BBa_E0030
annotation948622
1
BBa_E0030
range948622
1
124
846
annotation948620
1
BBa_B0034
range948620
1
106
117
annotation948608
1
BBa_R0065
range948608
1
1
97
annotation948637
1
BBa_B0012
range948637
1
943
983
annotation948627
1
BBa_B0010
range948627
1
855
934
BBa_K817000
1
BBa_K817000
SP1-GLP1
Yen-Der Li
2012-09-23T11:00:00Z
2015-05-08T01:13:28Z
false
false
_1075_
0
13572
9
Released HQ 2013
In stock
true
This is the GLP-1 coding part along with the signal peptide sequence.
SP1-GLP1
SP1-GLP1
false
annotation2201480
1
GLP-1
range2201480
1
65
162
annotation2201479
1
SP1
range2201479
1
1
64
BBa_K819012
1
LacIM
Luminesensor repressible LacIM generator
ZHANG Hong
2012-09-02T11:00:00Z
2015-05-08T01:13:29Z
false
false
_1077_
0
13785
9
Released HQ 2013
In stock
false
LacI mutant driven by luminesensor repressible SulA promoter in 408 form.
B0034, CDS of LacI mutant and B0015 were connected directly to SulA promoter in 408 form.
Sequene of the LacI mutant is from Partregistry.
false
annotation2181627
1
LacIM(K395400)
range2181627
1
65
1292
annotation2181628
1
SulA408 Promoter
range2181628
1
1
64
BBa_K779500
1
BBa_K779500
Long reporter top strand with bulge MammoBlock
Kristjan Eerik Kaseniit
2012-09-28T11:00:00Z
2015-05-08T01:13:18Z
false
false
_1033_
0
12684
9
Released HQ 2013
In stock
false
Reporter top strand with a 4nt bulge.
Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmC mAmCmU mUmCmU mCmCmC mAmCmC mA/3AlexF488N/
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature.
4-nucleotide bulge added 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011).
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
De novo design.
false
annotation2204234
1
Domain Sa part 2
range2204234
1
15
24
annotation2204231
1
Bulge
range2204231
1
11
14
annotation2204233
1
Domain Sa part 1
range2204233
1
1
10
annotation2204232
1
Domain Sb
range2204232
1
1
24
BBa_R0082
1
OmpR
Promoter (OmpR, positive)
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1.
An alternate version, BBa_R0083, cuts out the C2 and C3 sites.
The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
NC_000193 E. coli K12
true
annotation301156
1
C3 OmpR
range301156
1
54
71
annotation301167
1
-10
range301167
1
98
103
annotation301155
1
C2 OmpR
range301155
1
34
51
annotation301154
1
C1 OmpR
range301154
1
13
30
annotation301166
1
-35
range301166
1
75
80
BBa_I732095
1
BBa_I732095
Promoter Activity Reporter (LacZ-alpha and RFP)
Zhan Jian
2007-08-31T11:00:00Z
2015-08-31T04:07:57Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component2265792
1
BBa_I732074
component2265804
1
BBa_I13507
annotation2265792
1
BBa_I732074
range2265792
1
1
279
annotation2265804
1
BBa_I13507
range2265804
1
288
1148
BBa_B0040
1
spacer
Spacer.1 (generic)
Vinay S. Mahajan, Brian Chow, Peter Carr
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.</P>
<P> <P><p>The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the <a href="http://www.lifesci.ucsb.edu/~maduro/random.htm">Random DNA Generator </a>engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other <em>E.coli</em> sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. </p> <p> DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur):<br> T->A @ 85<br> T->A @ 42<br> C->T @ 79<br> A->T @ 64<br> A->T @ 31<br> T->A @ 34<br> C->A @ 37<br> Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences.<br> </p> <P>In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. </p><P> The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
Randomly generated and optimized for several parameters (see Design notes).
true
annotation1721
1
Spacer-1
range1721
1
1
70
annotation7030
1
BBa_B0040
range7030
1
1
70
BBa_I6207
1
BBa_I6207
Promoter O_H Test R0079.E0432
Jennifer Braff
2004-05-09T11:00:00Z
2015-08-31T04:07:44Z
false
false
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
false
component950605
1
BBa_B0012
component950595
1
BBa_B0010
component950570
1
BBa_R0079
component950575
1
BBa_B0034
component950587
1
BBa_E0032
annotation950587
1
BBa_E0032
range950587
1
184
945
annotation950605
1
BBa_B0012
range950605
1
1042
1082
annotation950595
1
BBa_B0010
range950595
1
954
1033
annotation950575
1
BBa_B0034
range950575
1
166
177
annotation950570
1
BBa_R0079
range950570
1
1
157
BBa_I13002
1
BBa_I13002
RBS B0031 w/ YFP (-LVA) TT
Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
2004-06-27T11:00:00Z
2015-08-31T04:07:32Z
false
false
_6_
0
101
7
Released HQ 2013
In stock
false
-- No description --
true
component943452
1
BBa_B0031
component943454
1
BBa_E0030
component943469
1
BBa_B0012
component943459
1
BBa_B0010
annotation943454
1
BBa_E0030
range943454
1
21
743
annotation943459
1
BBa_B0010
range943459
1
752
831
annotation943469
1
BBa_B0012
range943469
1
840
880
annotation943452
1
BBa_B0031
range943452
1
1
14
BBa_K863011
1
BBa_K863011
tthl laccase from Thermus thermophilus
Isabel Huber
2012-09-17T11:00:00Z
2015-05-08T01:13:36Z
false
false
_1123_
0
12844
9
Released HQ 2013
In stock
false
tthl (Laccase from Thermus thermophilus) coding sequence
test
Thermus thermophilus HB27
false
annotation2184806
1
ATG
range2184806
1
1
3
annotation2184888
1
tthl
range2184888
1
1
1389
annotation2184889
1
TAG
range2184889
1
1387
1389
BBa_A340620
1
BBa_A340620
Quadpart Amplifier (B0034.C0062.B0015.R0062)
cconboy
2004-08-09T11:00:00Z
2015-08-31T04:07:20Z
false
false
_11_1_
0
60
7
Released HQ 2013
In stock
false
-- No description --
false
component1041235
1
BBa_R0062
component1041202
1
BBa_C0062
component1041220
1
BBa_B0012
component1041187
1
BBa_B0034
component1041210
1
BBa_B0010
annotation1041202
1
BBa_C0062
range1041202
1
19
774
annotation1041187
1
BBa_B0034
range1041187
1
1
12
annotation1041220
1
BBa_B0012
range1041220
1
896
936
annotation1041235
1
BBa_R0062
range1041235
1
945
999
annotation1041210
1
BBa_B0010
range1041210
1
808
887
BBa_I746105
1
BBa_I746105
GFP with agr P2 promoter
Zhizhen Zhao
2007-09-06T11:00:00Z
2015-08-31T04:08:04Z
false
false
_116_
0
2121
9
Released HQ 2013
In stock
false
This is composed of a promoter and a GFP reporter gene. This can produce green fluorescence and in theory the strength of the green flourence is much stronger when there is phosphorylated AgrA in the cell. This is used to test the basal activity of agr P2 promoter.
NA
We used two subparts from the registry to produce the new part. BBa_I746104+BBa_E0840
false
component1943717
1
BBa_B0012
component1943711
1
BBa_B0030
component1943714
1
BBa_E0040
component1943715
1
BBa_B0010
component1943709
1
BBa_I746104
annotation1943709
1
BBa_I746104
range1943709
1
1
96
annotation1943714
1
BBa_E0040
range1943714
1
126
845
annotation1943715
1
BBa_B0010
range1943715
1
854
933
annotation1943717
1
BBa_B0012
range1943717
1
942
982
annotation1943711
1
BBa_B0030
range1943711
1
105
119
BBa_E0034
1
EYFP
enhanced yellow fluorescent protein derived from A. victoria GFP
jcbraff
2004-06-06T11:00:00Z
2015-08-31T04:07:25Z
false
false
_11_1_
0
61
7
Released HQ 2013
In stock
false
-- No description --
true
annotation1245672
1
AAV
range1245672
1
718
756
annotation1245671
1
stop
range1245671
1
757
762
BBa_J04450
1
BBa_J04450
RFP Coding Device
Tamar Odle
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
false
true
_16_
0
328
16
Released HQ 2013
In stock
false
Contains an IPTG inducible promoter an RBS, RFP, and terminator.
Davidson Synth-Aces
true
component1509411
1
BBa_E1010
component1509394
1
BBa_R0010
component1509427
1
BBa_B0012
component1509417
1
BBa_B0010
component1509404
1
BBa_B0034
annotation1509404
1
BBa_B0034
range1509404
1
209
220
annotation1509417
1
BBa_B0010
range1509417
1
941
1020
annotation1509394
1
BBa_R0010
range1509394
1
1
200
annotation1509411
1
BBa_E1010
range1509411
1
227
907
annotation1509427
1
BBa_B0012
range1509427
1
1029
1069
BBa_K876011
1
BBa_K876011
pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI
Timothy Cogan
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13836
9
Released HQ 2013
In stock
true
Population 2 for use with the dual strain toggle
Biobrick placement
Generated from biobricks
false
component2295837
1
BBa_C0079
component2295851
1
BBa_C0012
component2295844
1
BBa_B0015
component2295865
1
BBa_K081014
component2295866
1
BBa_R0010
component2295874
1
BBa_C0061
component2295828
1
BBa_R0040
component2295849
1
BBa_R0079
annotation2295849
1
BBa_R0079
range2295849
1
987
1143
annotation2295851
1
BBa_C0012
range2295851
1
1150
2277
annotation2295828
1
BBa_R0040
range2295828
1
1
54
annotation2295874
1
BBa_C0061
range2295874
1
3299
3916
annotation2295844
1
BBa_B0015
range2295844
1
850
978
annotation2295866
1
BBa_R0010
range2295866
1
3093
3292
annotation2295837
1
BBa_C0079
range2295837
1
61
841
annotation2295865
1
BBa_K081014
range2295865
1
2311
3084
BBa_K808012
1
BBa_K808012
tphA2: Catalyzes together with tphA3 TPA to DCD
Sascha Hein, Sven Rumpf, Daniel Sachs
2012-09-01T11:00:00Z
2015-05-08T01:13:25Z
false
false
_1065_
0
11792
9
Released HQ 2013
In stock
false
-
-
-
false
BBa_K861110
1
BBa_K861110
BcsB,regulator of cellulose synthetase
Xian Xia
2012-09-06T11:00:00Z
2015-06-17T01:21:30Z
false
false
_1121_
0
4206
12357
9
Released HQ 2013
In stock
false
BcsB is involved in biosynthesis of cellulose, which is a significant constituent of the extracellular matrix observed during multicellular morphotype (rdar) growth. The BcsB protein that was indirectly inferred to bind c-di-GMP.
None
Escherichia coli str. K-12 substr. MG1655
false
BBa_I13503
1
mRFP1
Screening plasmid intermediate
jkm
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
false
true
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction
false
component1505061
1
BBa_E1010
component1505053
1
BBa_B0032
annotation1505061
1
BBa_E1010
range1505061
1
20
700
annotation1505053
1
BBa_B0032
range1505053
1
1
13
BBa_I6080
1
BBa_I6080
Promoter O_H Test R1051.E0432
Caitlin Conboy and Jennifer Braff
2004-03-17T12:00:00Z
2015-08-31T04:07:43Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component949438
1
BBa_R1051
component949458
1
BBa_E0032
component949476
1
BBa_B0012
component949446
1
BBa_B0034
component949466
1
BBa_B0010
annotation949438
1
BBa_R1051
range949438
1
1
49
annotation949446
1
BBa_B0034
range949446
1
58
69
annotation949476
1
BBa_B0012
range949476
1
934
974
annotation949466
1
BBa_B0010
range949466
1
846
925
annotation949458
1
BBa_E0032
range949458
1
76
837
BBa_K777117
1
BBa_K777117
motB
Team Goettingen
2012-09-17T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
Released HQ 2013
In stock
false
This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
* motA was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
** Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAGAATCAAGCGCATCC
** Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACCTCGGTTCGGCTGATGG
* The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
false
annotation2184526
1
start
range2184526
1
1
1
annotation2184527
1
stop
range2184527
1
927
927
annotation2184525
1
MotB coding region
range2184525
1
1
927
BBa_J09855
1
BBa_J09855
Constitutive luxR device with pLuxR
Jon Badalamenti
2006-10-25T11:00:00Z
2015-08-31T04:08:21Z
false
true
_72_40_
0
475
72
Released HQ 2013
In stock
true
I14032.I0462.R0062
This is a universal part designed to allow induction by HSL of whatever is assembled downstream of the part.
This part was constructed from registry biobricks.
true
component2260447
1
BBa_R0062
component2260430
1
BBa_I14032
component2260445
1
BBa_I0462
annotation2260430
1
BBa_I14032
range2260430
1
1
37
annotation2260447
1
BBa_R0062
range2260447
1
990
1044
annotation2260445
1
BBa_I0462
range2260445
1
46
981
BBa_I13028
1
BBa_I13028
cI (TP-901) QPI intermediate
jenmitch
2004-07-14T11:00:00Z
2015-08-31T04:07:32Z
false
false
_6_
0
159
7
Released HQ 2013
In stock
false
-- No description --
false
component939472
1
BBa_C0075
component939462
1
BBa_B0034
annotation939462
1
BBa_B0034
range939462
1
1
12
annotation939472
1
BBa_C0075
range939472
1
19
597
BBa_J04421
1
BBa_J04421
ECFP Coding Device with Promoter, RBS, Coding Sequence, and Terminator
Kristen DeCelle
2005-06-08T11:00:00Z
2016-10-14T07:25:32Z
false
true
_16_
31248
326
16
Released HQ 2013
In stock
false
IPTG Inducible Promoter, RBS, ECFP with LVA tag, Terminator
false
component1509538
1
BBa_B0012
component1509520
1
BBa_E0022
component1509508
1
BBa_B0034
component1509528
1
BBa_B0010
component1509498
1
BBa_R0010
annotation1509528
1
BBa_B0010
range1509528
1
997
1076
annotation1509538
1
BBa_B0012
range1509538
1
1085
1125
annotation1509498
1
BBa_R0010
range1509498
1
1
200
annotation1509508
1
BBa_B0034
range1509508
1
209
220
annotation1509520
1
BBa_E0022
range1509520
1
227
988
BBa_K808033
1
BBa_K808033
araC-Pbad regulated XylE - catechol 2,3-dioxygenase from P.putida with terminator
Valentina Herbring, Sebastian Palluk, Andreas Schmidt
2012-09-05T11:00:00Z
2015-05-08T01:13:26Z
false
false
_1065_
0
12529
9
Released HQ 2013
In stock
false
-
-
-
false
component2201322
1
BBa_K808000
component2201335
1
BBa_K316003
component2201323
1
BBa_J61100
annotation2201322
1
BBa_K808000
range2201322
1
1
1209
annotation2201323
1
BBa_J61100
range2201323
1
1218
1229
annotation2201335
1
BBa_K316003
range2201335
1
1238
2298
BBa_I732074
1
BBa_I732074
Promoter Adaptor + LacZ-alpha Reporter
Zhan Jian
2007-08-01T11:00:00Z
2015-08-31T04:07:57Z
false
false
_156_
0
1557
9
Released HQ 2013
In stock
false
N/A
N/A
N/A
false
component1940656
1
BBa_B0034
component1940654
1
BBa_I732007
component1940660
1
BBa_I732006
annotation1940656
1
BBa_B0034
range1940656
1
28
39
annotation1940660
1
BBa_I732006
range1940660
1
46
279
annotation1940654
1
BBa_I732007
range1940654
1
1
19
BBa_J13023
1
BBa_J13023
3OC6HSL+LuxR dependent POPS/RIPS generator
Jeff Tabor
2005-06-28T11:00:00Z
2015-08-31T04:08:29Z
false
true
_37_
0
88
37
Released HQ 2013
In stock
false
produces pops and rips in the presence of luxR/3OC6HSL and the absence of lambda cI.
false
component1556583
1
BBa_B0034
component1556571
1
BBa_R0065
annotation1556583
1
BBa_B0034
range1556583
1
106
117
annotation1556571
1
BBa_R0065
range1556571
1
1
97
BBa_I13500
1
BBa_I13500
Screening plasmid intermediate
jkm
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
false
true
_11_
0
253
6
Released HQ 2013
In stock
false
Built by Josh as an intermediate in screening plasmid construction
true
component1505002
1
BBa_E0040
component1505000
1
BBa_B0034
annotation1505000
1
BBa_B0034
range1505000
1
1
12
annotation1505002
1
BBa_E0040
range1505002
1
19
738
BBa_I6031
1
BBa_I6031
Promoter O_H Test R0011.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component2221231
1
BBa_R0011
component2221245
1
BBa_E0430
annotation2221245
1
BBa_E0430
range2221245
1
64
941
annotation2221231
1
BBa_R0011
range2221231
1
1
54
BBa_I6103
1
BBa_I6103
Promoter O_H Test R0074.E0430
Randy Rettberg
2004-04-21T11:00:00Z
2015-08-31T04:07:44Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component949805
1
BBa_B0012
component949783
1
BBa_R0074
component949795
1
BBa_B0010
component949790
1
BBa_E0030
component949788
1
BBa_B0034
annotation949790
1
BBa_E0030
range949790
1
104
826
annotation949805
1
BBa_B0012
range949805
1
923
963
annotation949795
1
BBa_B0010
range949795
1
835
914
annotation949788
1
BBa_B0034
range949788
1
86
97
annotation949783
1
BBa_R0074
range949783
1
1
77
BBa_S01003
1
BBa_S01003
R0062.E0422
Randy Rettberg
2005-06-10T11:00:00Z
2015-05-08T01:14:16Z
false
true
_1_
0
25
1
Released HQ 2013
In stock
false
Intermediate part from Jen 1 tube 45
false
component1510982
1
BBa_B0034
component1510994
1
BBa_E0022
component1511012
1
BBa_B0012
component1510974
1
BBa_R0062
component1511002
1
BBa_B0010
annotation1510974
1
BBa_R0062
range1510974
1
1
55
annotation1510994
1
BBa_E0022
range1510994
1
82
843
annotation1510982
1
BBa_B0034
range1510982
1
64
75
annotation1511002
1
BBa_B0010
range1511002
1
852
931
annotation1511012
1
BBa_B0012
range1511012
1
940
980
BBa_S0108
1
BBa_S0108
B0033.C0050
Caitlin Conboy and Jennifer Braff
2003-12-04T12:00:00Z
2015-05-08T01:14:17Z
false
false
_1_
0
24
7
Released HQ 2013
In stock
false
false
component939848
1
BBa_C0050
component939832
1
BBa_B0033
annotation939832
1
BBa_B0033
range939832
1
1
11
annotation939848
1
BBa_C0050
range939848
1
18
761
BBa_K876014
1
BBa_K876014
pLac-LuxI
Timothy Cogan
2012-09-29T11:00:00Z
2015-05-08T01:13:38Z
false
false
_1138_
0
13836
9
Released HQ 2013
In stock
false
Constitutive promoter for LuxI which can be repressed by LacI
Biobrick placement
Generated from biobricks
false
component2245114
1
BBa_C0061
component2245106
1
BBa_R0010
annotation2245106
1
BBa_R0010
range2245106
1
1
200
annotation2245114
1
BBa_C0061
range2245114
1
207
824
BBa_I6030
1
BBa_I6030
Promoter O_H Test R0010.E0430
Caitlin Conboy and Jennifer Braff
2004-03-16T12:00:00Z
2015-08-31T04:07:43Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
-- No description --
false
component948302
1
BBa_B0012
component948292
1
BBa_B0010
component948285
1
BBa_B0034
component948275
1
BBa_R0010
component948287
1
BBa_E0030
annotation948287
1
BBa_E0030
range948287
1
227
949
annotation948285
1
BBa_B0034
range948285
1
209
220
annotation948292
1
BBa_B0010
range948292
1
958
1037
annotation948275
1
BBa_R0010
range948275
1
1
200
annotation948302
1
BBa_B0012
range948302
1
1046
1086
BBa_K750009
1
TD1.0
TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP
Sifan Wang,Ruosang Qiu,Xinyi Yao,Qingshu Wu
2012-09-18T11:00:00Z
2015-05-08T01:13:12Z
false
false
_1001_
0
14212
9
Released HQ 2013
In stock
true
This part contains BBa-K750001 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
editing
We built this part by biobricks from the DNA distribution kit plates 2012.
false
component2312646
1
BBa_K750007
component2312609
1
BBa_K750001
annotation2312646
1
BBa_K750007
range2312646
1
945
2964
annotation2312609
1
BBa_K750001
range2312609
1
1
936
BBa_K876070
1
BBa_K876070
CP-GFP-Term
Zakaraya Ashour, Sanket Shah
2012-10-01T11:00:00Z
2015-05-08T01:13:39Z
false
false
_1138_
0
13835
9
Released HQ 2013
In stock
false
Constitutive promoter with GFP
N/A
N/A
false
component2206856
1
BBa_J23103
component2206867
1
BBa_K081012
annotation2206856
1
BBa_J23103
range2206856
1
1
35
annotation2206867
1
BBa_K081012
range2206867
1
44
831
BBa_K801070
1
BBa_K801070
xanthosine methyltransferase CaXMT1-strep
Roman Prechtl
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
false
false
_1057_
0
11966
9
Released HQ 2013
In stock
false
xanthosine methyltransferase CaXMT1-strep from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
-
http://www.ncbi.nlm.nih.gov/nuccore/AB048793
synthesized by IDT, yeast codon optimized
false
annotation2198400
1
Strep Tag
range2198400
1
1123
1152
annotation2198398
1
CaXMT1
range2198398
1
7
1122
annotation2198397
1
Consensus sequence
range2198397
1
1
6
annotation2198399
1
Start codon
range2198399
1
7
9
annotation2198401
1
Stop codon
range2198401
1
1153
1158