iGEM 2019 Distribution Plate 4 Well 5D
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iGEM 2019 Distribution Plate 4 Well 17F
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iGEM 2019 Distribution Plate 4 Well 20I
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iGEM 2019 Distribution Plate 4 Well 8C
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iGEM 2019 Distribution Plate 4 Well 2A
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iGEM 2019 Distribution Plate 4 Well 5J
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iGEM 2019 Distribution Plate 4 Well 21A
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iGEM 2019 Distribution Plate 4 Well 21I
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iGEM 2019 Distribution Plate 4 Well 13O
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iGEM 2019 Distribution Plate 4 Well 11K
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iGEM 2019 Distribution Plate 4 Well 2B
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iGEM 2019 Distribution Plate 4 Well 18G
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iGEM 2019 Distribution Plate 4 Well 15G
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iGEM 2019 Distribution Plate 4 Well 2M
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iGEM 2019 Distribution Plate 4 Well 2G
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iGEM 2019 Distribution Plate 4 Well 17J
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iGEM 2019 Distribution Plate 4 Well 11H
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iGEM 2019 Distribution Plate 4 Well 4L
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iGEM 2019 Distribution Plate 4 Well 23H
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iGEM 2019 Distribution Plate 4 Well 22K
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iGEM 2019 Distribution Plate 4 Well 10G
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iGEM 2019 Distribution Plate 4 Well 1D
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iGEM 2019 Distribution Plate 4 Well 23J
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iGEM 2019 Distribution Plate 4 Well 7K
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iGEM 2019 Distribution Plate 4 Well 18K
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iGEM 2019 Distribution Plate 4 Well 14J
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iGEM 2019 Distribution Plate 4 Well 3D
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iGEM 2019 Distribution Plate 4 Well 6F
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iGEM 2019 Distribution Plate 4 Well 5K
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iGEM 2019 Distribution Plate 4 Well 2K
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iGEM 2019 Distribution Plate 4 Well 16L
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iGEM 2019 Distribution Plate 4 Well 7J
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iGEM 2019 Distribution Plate 4 Well 9C
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iGEM 2019 Distribution Plate 4 Well 17B
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iGEM 2019 Distribution Plate 4 Well 12L
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iGEM 2019 Distribution Plate 4 Well 14O
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iGEM 2019 Distribution Plate 4 Well 7O
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iGEM 2019 Distribution Plate 4 Well 17C
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iGEM 2019 Distribution Plate 4 Well 13J
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iGEM 2019 Distribution Plate 4 Well 20M
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iGEM 2019 Distribution Plate 4 Well 14A
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iGEM 2019 Distribution Plate 4 Well 16G
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iGEM 2019 Distribution Plate 4 Well 9F
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iGEM 2019 Distribution Plate 4 Well 9M
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iGEM 2019 Distribution Plate 4 Well 6O
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iGEM 2019 Distribution Plate 4 Well 20A
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iGEM 2019 Distribution Plate 4 Well 17D
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iGEM 2019 Distribution Plate 4 Well 7H
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iGEM 2019 Distribution Plate 4 Well 1C
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iGEM 2019 Distribution Plate 4 Well 21B
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iGEM 2019 Distribution Plate 4 Well 23L
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iGEM 2019 Distribution Plate 4 Well 1O
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iGEM 2019 Distribution Plate 4 Well 8D
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iGEM 2019 Distribution Plate 4 Well 1P
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iGEM 2019 Distribution Plate 4 Well 9E
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iGEM 2019 Distribution Plate 4 Well 9N
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iGEM 2019 Distribution Plate 4 Well 12A
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iGEM 2019 Distribution Plate 4 Well 4G
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iGEM 2019 Distribution Plate 4 Well 15L
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iGEM 2019 Distribution Plate 4 Well 9A
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iGEM 2019 Distribution Plate 4 Well 17I
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iGEM 2019 Distribution Plate 4 Well 19K
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iGEM 2019 Distribution Plate 4 Well 10E
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iGEM 2019 Distribution Plate 4 Well 20D
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iGEM 2019 Distribution Plate 4 Well 18O
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iGEM 2019 Distribution Plate 4 Well 12E
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iGEM 2019 Distribution Plate 4 Well 4B
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iGEM 2019 Distribution Plate 4 Well 16O
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iGEM 2019 Distribution Plate 4 Well 5P
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iGEM 2019 Distribution Plate 4 Well 2C
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iGEM 2019 Distribution Plate 4 Well 13M
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iGEM 2019 Distribution Plate 4 Well 19P
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iGEM 2019 Distribution Plate 4 Well 23O
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iGEM 2019 Distribution Plate 4 Well 4H
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iGEM 2019 Distribution Plate 4 Well 5O
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iGEM 2019 Distribution Plate 4 Well 5G
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iGEM 2019 Distribution Plate 4 Well 20K
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iGEM 2019 Distribution Plate 4 Well 19D
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member: BBa_K500001

iGEM 2019 Distribution Plate 4 Well 12N
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iGEM 2019 Distribution Plate 4 Well 8M
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iGEM 2019 Distribution Plate 4 Well 24E
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iGEM 2019 Distribution Plate 4 Well 15D
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iGEM 2019 Distribution Plate 4 Well 8N
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iGEM 2019 Distribution Plate 4 Well 9D
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iGEM 2019 Distribution Plate 4 Well 12P
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iGEM 2019 Distribution Plate 4 Well 6P
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iGEM 2019 Distribution Plate 4 Well 1B
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member: BBa_K249003

iGEM 2019 Distribution Plate 4 Well 1F
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member: BBa_K676004

iGEM 2019 Distribution Plate 4 Well 17H
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member: BBa_K116401

iGEM 2019 Distribution Plate 4 Well 6D
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iGEM 2019 Distribution Plate 4 Well 16M
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iGEM 2019 Distribution Plate 4 Well 10H
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iGEM 2019 Distribution Plate 4 Well 16F
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iGEM 2019 Distribution Plate 4 Well 20C
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iGEM 2019 Distribution Plate 4 Well 6L
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iGEM 2019 Distribution Plate 4 Well 10I
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iGEM 2019 Distribution Plate 4 Well 6N
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iGEM 2019 Distribution Plate 4 Well 9L
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iGEM 2019 Distribution Plate 4 Well 6A
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iGEM 2019 Distribution Plate 4 Well 2I
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iGEM 2019 Distribution Plate 4 Well 9O
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iGEM 2019 Distribution Plate 4 Well 9G
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iGEM 2019 Distribution Plate 4 Well 7C
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iGEM 2019 Distribution Plate 4 Well 17G
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iGEM 2019 Distribution Plate 4 Well 11F
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iGEM 2019 Distribution Plate 4 Well 9I
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iGEM 2019 Distribution Plate 4 Well 13F
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iGEM 2019 Distribution Plate 4 Well 14D
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member: BBa_P10100

iGEM 2019 Distribution Plate 4 Well 24D
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member: BBa_P10598

iGEM 2019 Distribution Plate 4 Well 12J
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iGEM 2019 Distribution Plate 4 Well 11J
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member: BBa_K530001

iGEM 2019 Distribution Plate 4 Well 19I
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member: BBa_K1051001

iGEM 2019 Distribution Plate 4 Well 9H
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member: BBa_K1031301

iGEM 2019 Distribution Plate 4 Well 19A
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iGEM 2019 Distribution Plate 4 Well 5B
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iGEM 2019 Distribution Plate 4 Well 16K
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iGEM 2019 Distribution Plate 4 Well 18B
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iGEM 2019 Distribution Plate 4 Well 8F
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iGEM 2019 Distribution Plate 4 Well 9J
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iGEM 2019 Distribution Plate 4 Well 6G
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iGEM 2019 Distribution Plate 4 Well 4K
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iGEM 2019 Distribution Plate 4 Well 5N
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iGEM 2019 Distribution Plate 4 Well 7E
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iGEM 2019 Distribution Plate 4 Well 7B
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iGEM 2019 Distribution Plate 4 Well 19N
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iGEM 2019 Distribution Plate 4 Well 13C
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iGEM 2019 Distribution Plate 4 Well 8H
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iGEM 2019 Distribution Plate 4 Well 22A
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iGEM 2019 Distribution Plate 4 Well 7D
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iGEM 2019 Distribution Plate 4 Well 14P
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iGEM 2019 Distribution Plate 4 Well 5M
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iGEM 2019 Distribution Plate 4 Well 1I
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iGEM 2019 Distribution Plate 4 Well 8E
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iGEM 2019 Distribution Plate 4 Well 18M
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iGEM 2019 Distribution Plate 4 Well 22O
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iGEM 2019 Distribution Plate 4 Well 22B
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iGEM 2019 Distribution Plate 4 Well 6K
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iGEM 2019 Distribution Plate 4 Well 2F
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iGEM 2019 Distribution Plate 4 Well 11A
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iGEM 2019 Distribution Plate 4 Well 12O
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iGEM 2019 Distribution Plate 4 Well 19F
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iGEM 2019 Distribution Plate 4 Well 11P
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iGEM 2019 Distribution Plate 4 Well 11E
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iGEM 2019 Distribution Plate 4 Well 15C
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iGEM 2019 Distribution Plate 4 Well 20B
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iGEM 2019 Distribution Plate 4 Well 2O
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iGEM 2019 Distribution Plate 4 Well 16H
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iGEM 2019 Distribution Plate 4 Well 13L
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iGEM 2019 Distribution Plate 4 Well 14M
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iGEM 2019 Distribution Plate 4 Well 7A
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iGEM 2019 Distribution Plate 4 Well 3E
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iGEM 2019 Distribution Plate 4 Well 1J
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iGEM 2019 Distribution Plate 4 Well 16I
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iGEM 2019 Distribution Plate 4 Well 16A
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iGEM 2019 Distribution Plate 4 Well 18H
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iGEM 2019 Distribution Plate 4 Well 8P
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iGEM 2019 Distribution Plate 4 Well 14N
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iGEM 2019 Distribution Plate 4 Well 13A
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iGEM 2019 Distribution Plate 4 Well 13I
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iGEM 2019 Distribution Plate 4 Well 15A
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iGEM 2019 Distribution Plate 4 Well 22M
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iGEM 2019 Distribution Plate 4 Well 10F
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iGEM 2019 Distribution Plate 4 Well 3N
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iGEM 2019 Distribution Plate 4 Well 16J
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iGEM 2019 Distribution Plate 4 Well 22H
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iGEM 2019 Distribution Plate 4 Well 19B
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iGEM 2019 Distribution Plate 4 Well 23P
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iGEM 2019 Distribution Plate 4 Well 10O
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iGEM 2019 Distribution Plate 4 Well 21M
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iGEM 2019 Distribution Plate 4 Well 3J
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iGEM 2019 Distribution Plate 4 Well 7I
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iGEM 2019 Distribution Plate 4 Well 2H
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iGEM 2019 Distribution Plate 4 Well 16N
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iGEM 2019 Distribution Plate 4 Well 4O
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iGEM 2019 Distribution Plate 4 Well 16C
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iGEM 2019 Distribution Plate 4 Well 1A
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iGEM 2019 Distribution Plate 4 Well 13D
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iGEM 2019 Distribution Plate 4 Well 19O
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iGEM 2019 Distribution Plate 4 Well 21D
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iGEM 2019 Distribution Plate 4 Well 13G
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iGEM 2019 Distribution Plate 4 Well 8B
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iGEM 2019 Distribution Plate 4 Well 12H
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iGEM 2019 Distribution Plate 4 Well 14B
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iGEM 2019 Distribution Plate 4 Well 15M
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iGEM 2019 Distribution Plate 4 Well 21H
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iGEM 2019 Distribution Plate 4 Well 20J
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iGEM 2019 Distribution Plate 4 Well 23I
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iGEM 2019 Distribution Plate 4
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iGEM 2019 Distribution Plate 4 Well 8O
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iGEM 2019 Distribution Plate 4 Well 13K
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iGEM 2019 Distribution Plate 4 Well 18E
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iGEM 2019 Distribution Plate 4 Well 4J
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iGEM 2019 Distribution Plate 4 Well 14F
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iGEM 2019 Distribution Plate 4 Well 21F
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iGEM 2019 Distribution Plate 4 Well 19M
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iGEM 2019 Distribution Plate 4 Well 14H
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iGEM 2019 Distribution Plate 4 Well 4N
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iGEM 2019 Distribution Plate 4 Well 1L
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iGEM 2019 Distribution Plate 4 Well 17K
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iGEM 2019 Distribution Plate 4 Well 24A
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iGEM 2019 Distribution Plate 4 Well 8G
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iGEM 2019 Distribution Plate 4 Well 5I
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iGEM 2019 Distribution Plate 4 Well 14C
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iGEM 2019 Distribution Plate 4 Well 5F
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iGEM 2019 Distribution Plate 4 Well 6J
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iGEM 2019 Distribution Plate 4 Well 4P
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iGEM 2019 Distribution Plate 4 Well 10C
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iGEM 2019 Distribution Plate 4 Well 13N
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iGEM 2019 Distribution Plate 4 Well 7P
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iGEM 2019 Distribution Plate 4 Well 17P
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iGEM 2019 Distribution Plate 4 Well 14L
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iGEM 2019 Distribution Plate 4 Well 18I
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iGEM 2019 Distribution Plate 4 Well 13E
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iGEM 2019 Distribution Plate 4 Well 19G
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member: BBa_K133139

iGEM 2019 Distribution Plate 4 Well 12B
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member: BBa_K953004

iGEM 2019 Distribution Plate 4 Well 20L
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iGEM 2019 Distribution Plate 4 Well 16B
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iGEM 2019 Distribution Plate 4 Well 10B
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iGEM 2019 Distribution Plate 4 Well 6M
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iGEM 2019 Distribution Plate 4 Well 13B
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iGEM 2019 Distribution Plate 4 Well 14I
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iGEM 2019 Distribution Plate 4 Well 24F
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iGEM 2019 Distribution Plate 4 Well 18A
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iGEM 2019 Distribution Plate 4 Well 6B
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iGEM 2019 Distribution Plate 4 Well 10K
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iGEM 2019 Distribution Plate 4 Well 20P
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iGEM 2019 Distribution Plate 4 Well 5C
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iGEM 2019 Distribution Plate 4 Well 4D
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iGEM 2019 Distribution Plate 4 Well 2L
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iGEM 2019 Distribution Plate 4 Well 11G
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iGEM 2019 Distribution Plate 4 Well 15J
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iGEM 2019 Distribution Plate 4 Well 7F
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iGEM 2019 Distribution Plate 4 Well 23D
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iGEM 2019 Distribution Plate 4 Well 10A
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iGEM 2019 Distribution Plate 4 Well 7N
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iGEM 2019 Distribution Plate 4 Well 4F
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iGEM 2019 Distribution Plate 4 Well 23A
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iGEM 2019 Distribution Plate 4 Well 24G
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iGEM 2019 Distribution Plate 4 Well 11O
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iGEM 2019 Distribution Plate 4 Well 1K
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iGEM 2019 Distribution Plate 4 Well 21K
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iGEM 2019 Distribution Plate 4 Well 22D
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iGEM 2019 Distribution Plate 4 Well 21J
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iGEM 2019 Distribution Plate 4 Well 17L
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iGEM 2019 Distribution Plate 4 Well 6I
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iGEM 2019 Distribution Plate 4 Well 22I
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iGEM 2019 Distribution Plate 4 Well 21L
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iGEM 2019 Distribution Plate 4 Well 5A
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iGEM 2019 Distribution Plate 4 Well 24O
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iGEM 2019 Distribution Plate 4 Well 13H
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iGEM 2019 Distribution Plate 4 Well 12K
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iGEM 2019 Distribution Plate 4 Well 8A
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iGEM 2019 Distribution Plate 4 Well 2P
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iGEM 2019 Distribution Plate 4 Well 20G
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iGEM 2019 Distribution Plate 4 Well 19H
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iGEM 2019 Distribution Plate 4 Well 17A
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iGEM 2019 Distribution Plate 4 Well 12M
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iGEM 2019 Distribution Plate 4 Well 22F
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iGEM 2019 Distribution Plate 4 Well 17N
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iGEM 2019 Distribution Plate 4 Well 10D
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iGEM 2019 Distribution Plate 4 Well 6E
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iGEM 2019 Distribution Plate 4 Well 2D
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iGEM 2019 Distribution Plate 4 Well 2E
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iGEM 2019 Distribution Plate 4 Well 15O
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iGEM 2019 Distribution Plate 4 Well 15P
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iGEM 2019 Distribution Plate 4 Well 3B
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iGEM 2019 Distribution Plate 4 Well 12D
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iGEM 2019 Distribution Plate 4 Well 15H
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iGEM 2019 Distribution Plate 4 Well 21G
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iGEM 2019 Distribution Plate 4 Well 4E
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iGEM 2019 Distribution Plate 4 Well 20O
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iGEM 2019 Distribution Plate 4 Well 19E
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iGEM 2019 Distribution Plate 4 Well 15B
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iGEM 2019 Distribution Plate 4 Well 1E
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iGEM 2019 Distribution Plate 4 Well 23F
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iGEM 2019 Distribution Plate 4 Well 22C
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iGEM 2019 Distribution Plate 4 Well 10N
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iGEM 2019 Distribution Plate 4 Well 11N
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iGEM 2019 Distribution Plate 4 Well 9B
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iGEM 2019 Distribution Plate 4 Well 11I
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iGEM 2019 Distribution Plate 4 Well 22G
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iGEM 2019 Distribution Plate 4 Well 6H
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iGEM 2019 Distribution Plate 4 Well 24H
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iGEM 2019 Distribution Plate 4 Well 15F
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iGEM 2019 Distribution Plate 4 Well 3M
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iGEM 2019 Distribution Plate 4 Well 18C
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iGEM 2019 Distribution Plate 4 Well 4M
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iGEM 2019 Distribution Plate 4 Well 18F
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iGEM 2019 Distribution Plate 4 Well 14K
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iGEM 2019 Distribution Plate 4 Well 24I
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iGEM 2019 Distribution Plate 4 Well 6C
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iGEM 2019 Distribution Plate 4 Well 2N
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iGEM 2019 Distribution Plate 4 Well 5E
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iGEM 2019 Distribution Plate 4 Well 11B
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persistent identity: iGEM 2019 Plate4 Well11B
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iGEM 2019 Distribution Plate 4 Well 11L
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iGEM 2019 Distribution Plate 4 Well 24C
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iGEM 2019 Distribution Plate 4 Well 3P
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iGEM 2019 Distribution Plate 4 Well 3K
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iGEM 2019 Distribution Plate 4 Well 3C
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iGEM 2019 Distribution Plate 4 Well 15E
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iGEM 2019 Distribution Plate 4 Well 9P
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iGEM 2019 Distribution Plate 4 Well 4A
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persistent identity: iGEM 2019 Plate4 Well4A
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display id: iGEM_2019_Plate4_Well7M
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iGEM 2019 Distribution Plate 4 Well 23G
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iGEM 2019 Distribution Plate 4 Well 23C
type: Collection
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type: Collection
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display id: iGEM_2019_Plate4_Well12I
version: 1
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iGEM 2019 Distribution Plate 4 Well 19J
type: Collection
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display id: iGEM_2019_Plate4_Well19J
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iGEM 2019 Distribution Plate 4 Well 9K
type: Collection
persistent identity: iGEM 2019 Plate4 Well9K
display id: iGEM_2019_Plate4_Well9K
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iGEM 2019 Distribution Plate 4 Well 12C
type: Collection
persistent identity: iGEM 2019 Plate4 Well12C
display id: iGEM_2019_Plate4_Well12C
version: 1
owned by: myers
member: BBa_K407008

BBa_K515105
type: ComponentDefinition
persistent identity: BBa K515105
display id: BBa_K515105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 promoter - sfGFP
creator: Atipat Patharagulpong
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part between J23100 and sfGFP
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K515005
sequence annotation: BBa_J23100
sequence annotation: BBa_K515005
sequence: https://synbiohub.org/public/igem/BBa_K515105_sequence/1

BBa_J23100
type: Component
persistent identity: component2219026
display id: component2219026
version: 1
top level: BBa_K515105
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K515005
type: Component
persistent identity: component2219029
display id: component2219029
version: 1
top level: BBa_K515105
access: public
definition: BBa_K515005

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2219026
display id: annotation2219026
version: 1
top level: BBa_K515105
location: https://synbiohub.org/public/igem/BBa_K515105/annotation2219026/range2219026/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K515105/annotation2219026/range2219026/1
type: Range
persistent identity: range2219026
display id: range2219026
version: 1
direction: direction/reverse
top level: BBa_K515105
start: 1
end: 35
orientation: inline

BBa_K515005
type: SequenceAnnotation
persistent identity: annotation2219029
display id: annotation2219029
version: 1
top level: BBa_K515105
location: https://synbiohub.org/public/igem/BBa_K515105/annotation2219029/range2219029/1
component: BBa_K515005

https://synbiohub.org/public/igem/BBa_K515105/annotation2219029/range2219029/1
type: Range
persistent identity: range2219029
display id: range2219029
version: 1
direction: direction/reverse
top level: BBa_K515105
start: 44
end: 787
orientation: inline

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
creator: Sabriya Rosemond, Erin Zwack
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
mutable notes: It was cloned into PsB1A2 plamsid.
mutable provenance: PsB1AT3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Rev primer J31007
sequence annotation: Fwd primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
direction: direction/forward
top level: tetA(C)f
start: 1
end: 1191
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
direction: direction/reverse
top level: tetA(C)f
start: 1172
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
direction: direction/forward
top level: tetA(C)f
start: 1
end: 24
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
direction: direction/forward
top level: tetA(C)f
start: 1189
end: 1191
orientation: inline

BBa_K515107
type: ComponentDefinition
persistent identity: BBa K515107
display id: BBa_K515107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Composite p(tetR) - Dendra2
creator: Atipat Patharagulpong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_R0040
component: BBa_K515007
sequence annotation: BBa_B0034
sequence annotation: BBa_K515007
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K515107_sequence/1

BBa_B0034
type: Component
persistent identity: component2128595
display id: component2128595
version: 1
top level: BBa_K515107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2128589
display id: component2128589
version: 1
top level: BBa_K515107
access: public
definition: p(tetR)

BBa_K515007
type: Component
persistent identity: component2128597
display id: component2128597
version: 1
top level: BBa_K515107
access: public
definition: https://synbiohub.org/public/igem/BBa_K515007/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128595
display id: annotation2128595
version: 1
top level: BBa_K515107
location: https://synbiohub.org/public/igem/BBa_K515107/annotation2128595/range2128595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K515107/annotation2128595/range2128595/1
type: Range
persistent identity: range2128595
display id: range2128595
version: 1
direction: direction/reverse
top level: BBa_K515107
start: 63
end: 74
orientation: inline

BBa_K515007
type: SequenceAnnotation
persistent identity: annotation2128597
display id: annotation2128597
version: 1
top level: BBa_K515107
location: https://synbiohub.org/public/igem/BBa_K515107/annotation2128597/range2128597/1
component: BBa_K515007

https://synbiohub.org/public/igem/BBa_K515107/annotation2128597/range2128597/1
type: Range
persistent identity: range2128597
display id: range2128597
version: 1
direction: direction/reverse
top level: BBa_K515107
start: 81
end: 761
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2128589
display id: annotation2128589
version: 1
top level: BBa_K515107
location: https://synbiohub.org/public/igem/BBa_K515107/annotation2128589/range2128589/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K515107/annotation2128589/range2128589/1
type: Range
persistent identity: range2128589
display id: range2128589
version: 1
direction: direction/forward
top level: BBa_K515107
start: 1
end: 54
orientation: inline

BBa_K315015
type: ComponentDefinition
persistent identity: BBa K315015
display id: BBa_K315015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetA gene (DeOptimized from bp 1 to 141)
creator: Danielle Wagner
created: 2010-07-05T11:00:00Z
modified: 2015-05-08T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _435_
m user id: 0
owner id: 7416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This sequence is the wild type TetA gene which has been deoptimized from base pairs 1 to 141. This is a basic part that can be placed behind a promoter and produce the Tetracycline resistance protein. Being that this gene is deoptimized, we are hypothesizing that it will allow for less translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis).
mutable notes: During the design of this sequence, it was necessary to ensure that the deoptimized sequence did not contain BioBrick restriction sites and that the translational sequence was identical to that of the wild type gene.
mutable provenance: This TetA gene has been deoptimized from base pairs 1 to 141 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/)and was constructed using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K315015_sequence/1

BBa_I732086
type: ComponentDefinition
persistent identity: BBa I732086
display id: BBa_I732086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [aTc] -> PoPS (version1)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is similar to [http://partsregistry.org/wiki/index.php/Part:BBa_I732083 BBa_I732083], but there is a mutation induced by PCR in [http://partsregistry.org/wiki/index.php/Part:BBa_I732084 BBa_I732084]. We are convinced that the mutation has nothing to do, and this part works as well as [http://partsregistry.org/wiki/index.php/Part:BBa_I732083 BBa_I732083].
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_I732084
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_I732084
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I732086_sequence/1

BBa_B0010
type: Component
persistent identity: component1948635
display id: component1948635
version: 1
top level: BBa_I732086
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I732084
type: Component
persistent identity: component1948634
display id: component1948634
version: 1
top level: BBa_I732086
access: public
definition: https://synbiohub.org/public/igem/BBa_I732084/1

BBa_B0012
type: Component
persistent identity: component1948637
display id: component1948637
version: 1
top level: BBa_I732086
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1948642
display id: component1948642
version: 1
top level: BBa_I732086
access: public
definition: p(tetR)

BBa_I732084
type: SequenceAnnotation
persistent identity: annotation1948634
display id: annotation1948634
version: 1
top level: BBa_I732086
location: https://synbiohub.org/public/igem/BBa_I732086/annotation1948634/range1948634/1
component: BBa_I732084

https://synbiohub.org/public/igem/BBa_I732086/annotation1948634/range1948634/1
type: Range
persistent identity: range1948634
display id: range1948634
version: 1
direction: direction/reverse
top level: BBa_I732086
start: 1
end: 689
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948637
display id: annotation1948637
version: 1
top level: BBa_I732086
location: https://synbiohub.org/public/igem/BBa_I732086/annotation1948637/range1948637/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732086/annotation1948637/range1948637/1
type: Range
persistent identity: range1948637
display id: range1948637
version: 1
direction: direction/forward
top level: BBa_I732086
start: 786
end: 826
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948635
display id: annotation1948635
version: 1
top level: BBa_I732086
location: https://synbiohub.org/public/igem/BBa_I732086/annotation1948635/range1948635/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732086/annotation1948635/range1948635/1
type: Range
persistent identity: range1948635
display id: range1948635
version: 1
direction: direction/forward
top level: BBa_I732086
start: 698
end: 777
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1948642
display id: annotation1948642
version: 1
top level: BBa_I732086
location: https://synbiohub.org/public/igem/BBa_I732086/annotation1948642/range1948642/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I732086/annotation1948642/range1948642/1
type: Range
persistent identity: range1948642
display id: range1948642
version: 1
direction: direction/forward
top level: BBa_I732086
start: 835
end: 888
orientation: inline

BBa_K531007
type: ComponentDefinition
persistent identity: BBa K531007
display id: BBa_K531007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PrsaA constitutive promoter, esp optimized for Caulobacter, and rsaA
creator: Alexander Aaring
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _697_
m user id: 0
owner id: 8840
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: PrsaA constitutive promoter from Caulobacter crescentus, esp, a suspected serine protease from Staphylococcus epidermidis, optimized for expression in Caulobacter crescentus, and the C-terminal secretion tag from rsaA.
mutable notes: Stop codon naturally occurring at the end of esp removed to ensure smooth expression of the fusion protein.
mutable provenance: PrsaA and rsaA C-terminal secretion tag both come from the genome of Caulobacter crescentus; esp optimized for Caulobacter crescentus is a synthetically codon optimized copy of esp as it is found in Staphylococcus epidermidis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K531006
component: BBa_K531001
sequence annotation: BBa_K531006
sequence annotation: BBa_K531001
sequence: https://synbiohub.org/public/igem/BBa_K531007_sequence/1

BBa_K531006
type: Component
persistent identity: component2136608
display id: component2136608
version: 1
top level: BBa_K531007
access: public
definition: BBa_K531006

BBa_K531001
type: Component
persistent identity: component2136609
display id: component2136609
version: 1
top level: BBa_K531007
access: public
definition: https://synbiohub.org/public/igem/BBa_K531001/1

BBa_K531006
type: SequenceAnnotation
persistent identity: annotation2136608
display id: annotation2136608
version: 1
top level: BBa_K531007
location: https://synbiohub.org/public/igem/BBa_K531007/annotation2136608/range2136608/1
component: BBa_K531006

https://synbiohub.org/public/igem/BBa_K531007/annotation2136608/range2136608/1
type: Range
persistent identity: range2136608
display id: range2136608
version: 1
direction: direction/reverse
top level: BBa_K531007
start: 96
end: 960
orientation: inline

BBa_K531001
type: SequenceAnnotation
persistent identity: annotation2136609
display id: annotation2136609
version: 1
top level: BBa_K531007
location: https://synbiohub.org/public/igem/BBa_K531007/annotation2136609/range2136609/1
component: BBa_K531001

https://synbiohub.org/public/igem/BBa_K531007/annotation2136609/range2136609/1
type: Range
persistent identity: range2136609
display id: range2136609
version: 1
direction: direction/reverse
top level: BBa_K531007
start: 969
end: 1328
orientation: inline

BBa_K500000
type: ComponentDefinition
persistent identity: BBa K500000
display id: BBa_K500000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lignin degradation 1
creator: Bin Jia
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _596_
m user id: 0
owner id: 4808
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: substrate:Non-phenolic
mutable notes: no
mutable provenance: P.chrysoporium lignin peroxidase isozyme H8 gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PRO
sequence annotation: Lip
sequence: https://synbiohub.org/public/igem/BBa_K500000_sequence/1

PRO
type: SequenceAnnotation
persistent identity: annotation2109134
display id: annotation2109134
version: 1
top level: BBa_K500000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K500000/annotation2109134/range2109134/1

https://synbiohub.org/public/igem/BBa_K500000/annotation2109134/range2109134/1
type: Range
persistent identity: range2109134
display id: range2109134
version: 1
direction: direction/forward
top level: BBa_K500000
start: 1
end: 3
orientation: inline

Lip
type: SequenceAnnotation
persistent identity: annotation2104117
display id: annotation2104117
version: 1
top level: BBa_K500000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K500000/annotation2104117/range2104117/1

https://synbiohub.org/public/igem/BBa_K500000/annotation2104117/range2104117/1
type: Range
persistent identity: range2104117
display id: range2104117
version: 1
direction: direction/forward
top level: BBa_K500000
start: 4
end: 1116
orientation: inline

BBa_K360127
type: ComponentDefinition
persistent identity: BBa K360127
display id: BBa_K360127
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23105 constitutive promoter + LovTAP Photosensor
creator: Claudia Ivonne Hernandez Armenta
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _485_
m user id: 0
owner id: 6618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This photoreceptor was assembled by Strickland et al., and consists of a LOV (light-oxygen-voltage) domain of Avena sativa phototropin1 (AsLOV2) that senses blue light, fused to the trpR- DNA binding domain of the transcription factor trp repressor. The resulting protein is called LovTAP: LOV- and tryptophan-activated protein. LOV domains bind a flavin-mononucleotide (FMN) or flavin-adenine-dinucleotide (FAD) cofactor, which are used in a wide variety of metabolic pathways as cofactors in redox reactions and are available in most organisms. The cofactor has a broad absorption spectrum, with a maximum at 450 nm. Besides, the core of the LOV domain is often flanked by amino- or carboxy-terminal helices, termed A???α and Jα, respectively. In the LovTAP construction, AsLOV2 domain via its carboxyl-terminal Jα ???helix was ligated to an amino-terminal truncation of TrpR. The resulting protein has a domain-domain overlap with a shared helix. Thus, photoexcitation would change the conformation of the protein, in turn changing the stability of the shared-helix-domain contacts. Under the presence of light, absorption of a photon leads to the formation of a covalent adduct between the flavin mononucleotide (FMN) cofactor and a conserved cysteine residue in the AsLOV2 domain, which results in conformational rearrangements in LovTAP. This change impacts the affinity of the shared helix for the two domains: disrupting the contacts between the shared helix and the LOV domain and enabling the association of the shared helix with the TrpR domain, which establishes DNA-binding affinity and LovTAP can then bind DNA as an homodimer, repressing the transcription of the genes downstream of its binding sites. In the dark, when the shared helix contacts the LOV domain, the TrpR domain's DNA-binding affinity decreases and LovTAP is in an inactive conformation.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K360121
component: BBa_J23105
sequence annotation: BBa_K360121
sequence annotation: BBa_J23105
sequence: https://synbiohub.org/public/igem/BBa_K360127_sequence/1

BBa_K360121
type: Component
persistent identity: component2091928
display id: component2091928
version: 1
top level: BBa_K360127
access: public
definition: BBa_K360121

BBa_J23105
type: Component
persistent identity: component2091921
display id: component2091921
version: 1
top level: BBa_K360127
access: public
definition: https://synbiohub.org/public/igem/BBa_J23105/1

BBa_K360121
type: SequenceAnnotation
persistent identity: annotation2091928
display id: annotation2091928
version: 1
top level: BBa_K360127
location: https://synbiohub.org/public/igem/BBa_K360127/annotation2091928/range2091928/1
component: BBa_K360121

https://synbiohub.org/public/igem/BBa_K360127/annotation2091928/range2091928/1
type: Range
persistent identity: range2091928
display id: range2091928
version: 1
direction: direction/reverse
top level: BBa_K360127
start: 44
end: 887
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2091921
display id: annotation2091921
version: 1
top level: BBa_K360127
location: https://synbiohub.org/public/igem/BBa_K360127/annotation2091921/range2091921/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K360127/annotation2091921/range2091921/1
type: Range
persistent identity: range2091921
display id: range2091921
version: 1
direction: direction/reverse
top level: BBa_K360127
start: 1
end: 35
orientation: inline

BBa_K625000
type: ComponentDefinition
persistent identity: BBa K625000
display id: BBa_K625000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-LacIM1
creator: Michael Eichenberger
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
group u list: _797_
m user id: 0
owner id: 10153
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Contains a rather weak RBS in front of a codon modified variant of LacI. This reduces the likelihood of recombination with wild type LacI when used in multi plasmid systems.
mutable notes: The RBS was chosen to match the one in [Basu et al, 2005] in order to create a working band pass filter. Minimal RBS length was chosen to be 40 bp in accordance with [Salis et al, 2009].
mutable provenance: PCR on DNA synthesized by GeneArt
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: LacI M1
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K625000_sequence/1

LacI M1
type: SequenceAnnotation
persistent identity: annotation2136636
display id: annotation2136636
version: 1
top level: BBa_K625000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K625000/annotation2136636/range2136636/1

https://synbiohub.org/public/igem/BBa_K625000/annotation2136636/range2136636/1
type: Range
persistent identity: range2136636
display id: range2136636
version: 1
direction: direction/forward
top level: BBa_K625000
start: 36
end: 1121
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136635
display id: annotation2136635
version: 1
top level: BBa_K625000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K625000/annotation2136635/range2136635/1

https://synbiohub.org/public/igem/BBa_K625000/annotation2136635/range2136635/1
type: Range
persistent identity: range2136635
display id: range2136635
version: 1
direction: direction/forward
top level: BBa_K625000
start: 1
end: 35
orientation: inline

BBa_K1031510
type: ComponentDefinition
persistent identity: BBa K1031510
display id: BBa_K1031510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HpaR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: HpaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: HpaR
sequence: https://synbiohub.org/public/igem/BBa_K1031510_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357469
display id: annotation2357469
version: 1
top level: BBa_K1031510
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1
type: Range
persistent identity: range2357469
display id: range2357469
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357473
display id: annotation2357473
version: 1
top level: BBa_K1031510
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1
type: Range
persistent identity: range2357473
display id: range2357473
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 474
end: 602
orientation: inline

HpaR
type: SequenceAnnotation
persistent identity: annotation2357472
display id: annotation2357472
version: 1
top level: BBa_K1031510
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1
type: Range
persistent identity: range2357472
display id: range2357472
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 19
end: 465
orientation: inline

BBa_K1051002
type: ComponentDefinition
persistent identity: BBa K1051002
display id: BBa_K1051002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stop codon free EYFP in RFC[23] standard
creator: Xiang LI
created: 2013-07-03T11:00:00Z
modified: 2015-05-08T01:08:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15912
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: EYFP without stop coding
mutable notes: add prefix and suffix del stop coding
mutable provenance: kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1051002_sequence/1

mCherry
type: ComponentDefinition
persistent identity: BBa J18932
display id: BBa_J18932
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry RFP
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Red fluorescent protein derived from DsRed. '''Advantages:''' * fast folding and maturation * bright and photo-stable
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18932_sequence/1

BBa_P10400
type: ComponentDefinition
persistent identity: BBa P10400
display id: BBa_P10400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3U+Ter-CaMV35S
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T06:02:16Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP41414
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10400_sequence/1

BBa_K258001
type: ComponentDefinition
persistent identity: BBa K258001
display id: BBa_K258001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lipase ABC transporter recognition domain (LARD 1)
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lard 1 was designed for the secretion of fusion proteins.The LARD 1 included four glycine-rich repeats comprising a β-roll structure, and were added to the C-terminus of test proteins. Either Pro-Gly linker or Factor Xa site can be added between fusion proteins and LARD 1. Upon supplementation of E. coli with ABC transporter from E. chrysanthemi (ABC transporter PrtDEF), which function well in the phylogenetically-neighboring genus E.coli, EGF-LARDs were excreted into the culture supernatant.
mutable notes: In our designed LARD 1, we favoured not to add these cleavage site so if you want, yo can add either Pro-Gly linker or Factor Xa site. Secretion of fusion proteins with LARDs could be detected by Western blotting. Fusion proteins were traced in the culture supernatant using antibody against LARDs. Polyclonal antibodies against the C-terminal signal sequence were produced with a synthetic peptide (YQPTDRLVFQGADGST, residues 421???436 of TliA ,also of LARD).
mutable provenance: LARD 1 composed of residues 303-476 of thermostable lipase (TliA) of Pseudomonas fluorescens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K258001_sequence/1

LRLb
type: ComponentDefinition
persistent identity: BBa I732102
display id: BBa_I732102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLb
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732102_sequence/1

BBa_K091137
type: ComponentDefinition
persistent identity: BBa K091137
display id: BBa_K091137
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+Mnt+TT
creator: Robert Cool
created: 2008-07-13T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was made by ligating I13025 to B0015.
mutable notes: no special cosiderations
mutable provenance: These parts can be found in the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13025
component: BBa_B0015
sequence annotation: BBa_I13025
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K091137_sequence/1

BBa_I13025
type: Component
persistent identity: component2249111
display id: component2249111
version: 1
top level: BBa_K091137
access: public
definition: https://synbiohub.org/public/igem/BBa_I13025/1

BBa_B0015
type: Component
persistent identity: component2249118
display id: component2249118
version: 1
top level: BBa_K091137
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I13025
type: SequenceAnnotation
persistent identity: annotation2249111
display id: annotation2249111
version: 1
top level: BBa_K091137
location: https://synbiohub.org/public/igem/BBa_K091137/annotation2249111/range2249111/1
component: BBa_I13025

https://synbiohub.org/public/igem/BBa_K091137/annotation2249111/range2249111/1
type: Range
persistent identity: range2249111
display id: range2249111
version: 1
direction: direction/reverse
top level: BBa_K091137
start: 1
end: 331
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249118
display id: annotation2249118
version: 1
top level: BBa_K091137
location: https://synbiohub.org/public/igem/BBa_K091137/annotation2249118/range2249118/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K091137/annotation2249118/range2249118/1
type: Range
persistent identity: range2249118
display id: range2249118
version: 1
direction: direction/reverse
top level: BBa_K091137
start: 340
end: 468
orientation: inline

cph8
type: ComponentDefinition
persistent identity: BBa K592000
display id: BBa_K592000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cph8 (Cph1/EnvZ fusion)
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is identical to BBa_I15010, the cph8 red light sensor. We were unable to transform BBa_I15010 from the the 2011 iGEM distribution kit. PCR attempts using VF2 and VR failed to amplify any DNA from the well sample. In the end, we chose to amplify cph8 from Jeffrey Tabor's pJT122 plasmid provided by Chris Voigt. The cph8 in pJT122 contained an internal PstI site, which was removed by point-mutagenesis. The resultant DNA sequence is identical to that of BBa_I15010.
mutable notes: The source DNA (pJT122) contained illegal restriction site (PstI) that had to be removed.
mutable provenance: Jeffrey Tabor's pJT122 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cph8
sequence: https://synbiohub.org/public/igem/BBa_K592000_sequence/1

cph8
type: SequenceAnnotation
persistent identity: annotation2132074
display id: annotation2132074
version: 1
top level: cph8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592000/annotation2132074/range2132074/1

https://synbiohub.org/public/igem/BBa_K592000/annotation2132074/range2132074/1
type: Range
persistent identity: range2132074
display id: range2132074
version: 1
direction: direction/forward
top level: cph8
start: 1
end: 2238
orientation: inline

BBa_K274371
type: ComponentDefinition
persistent identity: BBa K274371
display id: BBa_K274371
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> P2 ogr - PO promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746361
component: BBa_I746350
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746361
sequence annotation: BBa_I746350
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K274371_sequence/1

BBa_I746361
type: Component
persistent identity: component2055302
display id: component2055302
version: 1
top level: BBa_K274371
access: public
definition: https://synbiohub.org/public/igem/BBa_I746361/1

BBa_I746350
type: Component
persistent identity: component2055294
display id: component2055294
version: 1
top level: BBa_K274371
access: public
definition: https://synbiohub.org/public/igem/BBa_I746350/1

BBa_B0012
type: Component
persistent identity: component2055297
display id: component2055297
version: 1
top level: BBa_K274371
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2055295
display id: component2055295
version: 1
top level: BBa_K274371
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055297
display id: annotation2055297
version: 1
top level: BBa_K274371
location: https://synbiohub.org/public/igem/BBa_K274371/annotation2055297/range2055297/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274371/annotation2055297/range2055297/1
type: Range
persistent identity: range2055297
display id: range2055297
version: 1
direction: direction/forward
top level: BBa_K274371
start: 334
end: 374
orientation: inline

BBa_I746361
type: SequenceAnnotation
persistent identity: annotation2055302
display id: annotation2055302
version: 1
top level: BBa_K274371
location: https://synbiohub.org/public/igem/BBa_K274371/annotation2055302/range2055302/1
component: BBa_I746361

https://synbiohub.org/public/igem/BBa_K274371/annotation2055302/range2055302/1
type: Range
persistent identity: range2055302
display id: range2055302
version: 1
direction: direction/reverse
top level: BBa_K274371
start: 383
end: 474
orientation: inline

BBa_I746350
type: SequenceAnnotation
persistent identity: annotation2055294
display id: annotation2055294
version: 1
top level: BBa_K274371
location: https://synbiohub.org/public/igem/BBa_K274371/annotation2055294/range2055294/1
component: BBa_I746350

https://synbiohub.org/public/igem/BBa_K274371/annotation2055294/range2055294/1
type: Range
persistent identity: range2055294
display id: range2055294
version: 1
direction: direction/reverse
top level: BBa_K274371
start: 1
end: 237
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055295
display id: annotation2055295
version: 1
top level: BBa_K274371
location: https://synbiohub.org/public/igem/BBa_K274371/annotation2055295/range2055295/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274371/annotation2055295/range2055295/1
type: Range
persistent identity: range2055295
display id: range2055295
version: 1
direction: direction/forward
top level: BBa_K274371
start: 246
end: 325
orientation: inline

BBa_K676004
type: ComponentDefinition
persistent identity: BBa K676004
display id: BBa_K676004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Supercoilometer
creator: Ejaj Intisar
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _859_
m user id: 0
owner id: 10148
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: A device to detected the level of negative supercoils in a circular plasmid.
mutable notes: Primer and 3 way ligation
mutable provenance: E Coli promoter and iGEM kit plate CFP expression casette
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0422
component: BBa_K676003
sequence annotation: BBa_K676003
sequence annotation: BBa_E0422
sequence: https://synbiohub.org/public/igem/BBa_K676004_sequence/1

BBa_E0422
type: Component
persistent identity: component2127150
display id: component2127150
version: 1
top level: BBa_K676004
access: public
definition: https://synbiohub.org/public/igem/BBa_E0422/1

BBa_K676003
type: Component
persistent identity: component2127136
display id: component2127136
version: 1
top level: BBa_K676004
access: public
definition: https://synbiohub.org/public/igem/BBa_K676003/1

BBa_K676003
type: SequenceAnnotation
persistent identity: annotation2127136
display id: annotation2127136
version: 1
top level: BBa_K676004
location: https://synbiohub.org/public/igem/BBa_K676004/annotation2127136/range2127136/1
component: BBa_K676003

https://synbiohub.org/public/igem/BBa_K676004/annotation2127136/range2127136/1
type: Range
persistent identity: range2127136
display id: range2127136
version: 1
direction: direction/reverse
top level: BBa_K676004
start: 1
end: 103
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2127150
display id: annotation2127150
version: 1
top level: BBa_K676004
location: https://synbiohub.org/public/igem/BBa_K676004/annotation2127150/range2127150/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_K676004/annotation2127150/range2127150/1
type: Range
persistent identity: range2127150
display id: range2127150
version: 1
direction: direction/reverse
top level: BBa_K676004
start: 112
end: 1028
orientation: inline

BBa_K527002
type: ComponentDefinition
persistent identity: BBa K527002
display id: BBa_K527002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosynthesis/Oximes/CYP79B1/Plug 'n' Play
creator: Eva Meier Carlsen
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _693_
m user id: 0
owner id: 8631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick BBa_K527002 encode the plant enzyme Cytochrome P450 CYP79B1. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation. CYP79B1 is an N-hydroxylase converting L-Tryptophan into indole 3-acetaldoxime, the precursor of a cyanogenic glycoside . Oximes as the indole 3-acetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species. BBa_K527002 is submitted in the plasmid BBa_K678023 due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.
mutable notes: Mutated at a Pst1 restiction site (nucleotide 342) No prefix and suffix. User assembly ends.
mutable provenance: Institute of Plant Biology and Biotechnology Faculty of LIFE Science University of Copenhagen Denmark http://2011.igem.org/Team:DTU-Denmark-2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CYP79B1
sequence annotation: mutation
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K527002_sequence/1

CYP79B1
type: SequenceAnnotation
persistent identity: annotation2131870
display id: annotation2131870
version: 1
top level: BBa_K527002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K527002/annotation2131870/range2131870/1

https://synbiohub.org/public/igem/BBa_K527002/annotation2131870/range2131870/1
type: Range
persistent identity: range2131870
display id: range2131870
version: 1
direction: direction/forward
top level: BBa_K527002
start: 1
end: 1560
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2131869
display id: annotation2131869
version: 1
top level: BBa_K527002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K527002/annotation2131869/range2131869/1

https://synbiohub.org/public/igem/BBa_K527002/annotation2131869/range2131869/1
type: Range
persistent identity: range2131869
display id: range2131869
version: 1
direction: direction/forward
top level: BBa_K527002
start: 852
end: 852
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2131868
display id: annotation2131868
version: 1
top level: BBa_K527002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K527002/annotation2131868/range2131868/1

https://synbiohub.org/public/igem/BBa_K527002/annotation2131868/range2131868/1
type: Range
persistent identity: range2131868
display id: range2131868
version: 1
direction: direction/forward
top level: BBa_K527002
start: 1558
end: 1560
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2131865
display id: annotation2131865
version: 1
top level: BBa_K527002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K527002/annotation2131865/range2131865/1

https://synbiohub.org/public/igem/BBa_K527002/annotation2131865/range2131865/1
type: Range
persistent identity: range2131865
display id: range2131865
version: 1
direction: direction/forward
top level: BBa_K527002
start: 1
end: 3
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible pBad/araC promoter
creator: Sri Kosuri
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PC
sequence annotation: AraC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
direction: direction/forward
top level: pBad/araC
start: 1043
end: 1114
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
direction: direction/forward
top level: pBad/araC
start: 1
end: 1079
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
direction: direction/forward
top level: pBad/araC
start: 1080
end: 1210
orientation: inline

BBa_K1230007
type: ComponentDefinition
persistent identity: BBa K1230007
display id: BBa_K1230007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-pBad-rbs-LL37
creator: Andr??s Ferri??o Iriarte
created: 2013-09-17T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
group u list: _1544_
m user id: 0
owner id: 17080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Antimicrobial peptide generator (LL-37 with codon usage for E. coli)
mutable notes: Specific codon usage for E. coli. It works under arabinose induction.
mutable provenance: 2013 DNA distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K808000
component: BBa_B0034
component: BBa_K875009
sequence annotation: BBa_K875009
sequence annotation: BBa_B0034
sequence annotation: BBa_K808000
sequence: https://synbiohub.org/public/igem/BBa_K1230007_sequence/1

BBa_K808000
type: Component
persistent identity: component2353898
display id: component2353898
version: 1
top level: BBa_K1230007
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_B0034
type: Component
persistent identity: component2353900
display id: component2353900
version: 1
top level: BBa_K1230007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K875009
type: Component
persistent identity: component2353902
display id: component2353902
version: 1
top level: BBa_K1230007
access: public
definition: https://synbiohub.org/public/igem/BBa_K875009/1

BBa_K875009
type: SequenceAnnotation
persistent identity: annotation2353902
display id: annotation2353902
version: 1
top level: BBa_K1230007
location: https://synbiohub.org/public/igem/BBa_K1230007/annotation2353902/range2353902/1
component: BBa_K875009

https://synbiohub.org/public/igem/BBa_K1230007/annotation2353902/range2353902/1
type: Range
persistent identity: range2353902
display id: range2353902
version: 1
direction: direction/reverse
top level: BBa_K1230007
start: 1236
end: 1358
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2353900
display id: annotation2353900
version: 1
top level: BBa_K1230007
location: https://synbiohub.org/public/igem/BBa_K1230007/annotation2353900/range2353900/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1230007/annotation2353900/range2353900/1
type: Range
persistent identity: range2353900
display id: range2353900
version: 1
direction: direction/reverse
top level: BBa_K1230007
start: 1218
end: 1229
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2353898
display id: annotation2353898
version: 1
top level: BBa_K1230007
location: https://synbiohub.org/public/igem/BBa_K1230007/annotation2353898/range2353898/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1230007/annotation2353898/range2353898/1
type: Range
persistent identity: range2353898
display id: range2353898
version: 1
direction: direction/reverse
top level: BBa_K1230007
start: 1
end: 1209
orientation: inline

BBa_P10201
type: ComponentDefinition
persistent identity: BBa P10201
display id: BBa_P10201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 5U-TMV+SP-chloroplast
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:52:11Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP78133
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10201_sequence/1

BBa_K404319
type: ComponentDefinition
persistent identity: BBa K404319
display id: BBa_K404319
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cyan fluorescent protein (CFP) in RFC25 standard
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CFP
sequence: https://synbiohub.org/public/igem/BBa_K404319_sequence/1

CFP
type: SequenceAnnotation
persistent identity: annotation2094839
display id: annotation2094839
version: 1
top level: BBa_K404319
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404319/annotation2094839/range2094839/1

https://synbiohub.org/public/igem/BBa_K404319/annotation2094839/range2094839/1
type: Range
persistent identity: range2094839
display id: range2094839
version: 1
direction: direction/forward
top level: BBa_K404319
start: 1
end: 714
orientation: inline

BBa_K608014
type: ComponentDefinition
persistent identity: BBa K608014
display id: BBa_K608014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong promoter and medium RBS with RFP
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promtor,RBS with RFP
mutable notes: composite
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E1010
component: BBa_K608003
sequence annotation: BBa_K608003
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K608014_sequence/1

BBa_E1010
type: Component
persistent identity: component2247900
display id: component2247900
version: 1
top level: BBa_K608014
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K608003
type: Component
persistent identity: component2247897
display id: component2247897
version: 1
top level: BBa_K608014
access: public
definition: https://synbiohub.org/public/igem/BBa_K608003/1

BBa_K608003
type: SequenceAnnotation
persistent identity: annotation2247897
display id: annotation2247897
version: 1
top level: BBa_K608014
location: https://synbiohub.org/public/igem/BBa_K608014/annotation2247897/range2247897/1
component: BBa_K608003

https://synbiohub.org/public/igem/BBa_K608014/annotation2247897/range2247897/1
type: Range
persistent identity: range2247897
display id: range2247897
version: 1
direction: direction/reverse
top level: BBa_K608014
start: 1
end: 56
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247900
display id: annotation2247900
version: 1
top level: BBa_K608014
location: https://synbiohub.org/public/igem/BBa_K608014/annotation2247900/range2247900/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K608014/annotation2247900/range2247900/1
type: Range
persistent identity: range2247900
display id: range2247900
version: 1
direction: direction/reverse
top level: BBa_K608014
start: 63
end: 768
orientation: inline

BBa_P10599
type: ComponentDefinition
persistent identity: BBa P10599
display id: BBa_P10599
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenBraid destination vector alpha insert
creator: Vinoo Selvarajah
created: 2016-06-16T11:00:00Z
modified: 2016-06-17T03:42:33Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 4206
owner id: 4206
owning group id: 1
status: status/Planning
bookmark: false
mutable description: More information to come
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10599_sequence/1

MotB
type: ComponentDefinition
persistent identity: BBa S03271
display id: BBa_S03271
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MotB: RBS+flagellar protein
creator: Lane Weaver
created: 2005-07-24T11:00:00Z
modified: 2015-05-08T01:14:20Z
discontinued: false
dominant: false
group u list: _40_
m user id: 0
owner id: 335
owning group id: 40
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. coli flagellar protein linking torque machinery to cell wall; allows for induction of motility
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_S03271_sequence/1

BBa_K339007
type: ComponentDefinition
persistent identity: BBa K339007
display id: BBa_K339007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR Reporter
creator: Emily Hicks
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _460_
m user id: 0
owner id: 4377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: c j
mutable notes: c jks
mutable provenance: c skj
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K135000
component: BBa_I13507
sequence annotation: BBa_K135000
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K339007_sequence/1

BBa_K135000
type: Component
persistent identity: component2100025
display id: component2100025
version: 1
top level: BBa_K339007
access: public
definition: https://synbiohub.org/public/igem/BBa_K135000/1

BBa_I13507
type: Component
persistent identity: component2100037
display id: component2100037
version: 1
top level: BBa_K339007
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation2100025
display id: annotation2100025
version: 1
top level: BBa_K339007
location: https://synbiohub.org/public/igem/BBa_K339007/annotation2100025/range2100025/1
component: BBa_K135000

https://synbiohub.org/public/igem/BBa_K339007/annotation2100025/range2100025/1
type: Range
persistent identity: range2100025
display id: range2100025
version: 1
direction: direction/reverse
top level: BBa_K339007
start: 1
end: 55
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2100037
display id: annotation2100037
version: 1
top level: BBa_K339007
location: https://synbiohub.org/public/igem/BBa_K339007/annotation2100037/range2100037/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K339007/annotation2100037/range2100037/1
type: Range
persistent identity: range2100037
display id: range2100037
version: 1
direction: direction/reverse
top level: BBa_K339007
start: 64
end: 924
orientation: inline

BBa_K196004
type: ComponentDefinition
persistent identity: BBa K196004
display id: BBa_K196004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HfsG + HfsH proteins from Caulobacter crescentus
creator: Laetitia Warny
created: 2009-08-12T11:00:00Z
modified: 2015-05-08T01:11:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH [2] is a carbohydrate esterase. Here you have a part made of the Biobricks HfsG (BBa_K196002) and HfsH (BBa_K196003).
mutable notes: As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt [3]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.
mutable provenance: This sequence comes from Caulobacter crescentus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K196002
component: BBa_K196003
sequence annotation: BBa_K196002
sequence annotation: BBa_K196003
sequence: https://synbiohub.org/public/igem/BBa_K196004_sequence/1

BBa_K196002
type: Component
persistent identity: component2017248
display id: component2017248
version: 1
top level: BBa_K196004
access: public
definition: https://synbiohub.org/public/igem/BBa_K196002/1

BBa_K196003
type: Component
persistent identity: component2017249
display id: component2017249
version: 1
top level: BBa_K196004
access: public
definition: https://synbiohub.org/public/igem/BBa_K196003/1

BBa_K196002
type: SequenceAnnotation
persistent identity: annotation2017248
display id: annotation2017248
version: 1
top level: BBa_K196004
location: https://synbiohub.org/public/igem/BBa_K196004/annotation2017248/range2017248/1
component: BBa_K196002

https://synbiohub.org/public/igem/BBa_K196004/annotation2017248/range2017248/1
type: Range
persistent identity: range2017248
display id: range2017248
version: 1
direction: direction/reverse
top level: BBa_K196004
start: 1
end: 933
orientation: inline

BBa_K196003
type: SequenceAnnotation
persistent identity: annotation2017249
display id: annotation2017249
version: 1
top level: BBa_K196004
location: https://synbiohub.org/public/igem/BBa_K196004/annotation2017249/range2017249/1
component: BBa_K196003

https://synbiohub.org/public/igem/BBa_K196004/annotation2017249/range2017249/1
type: Range
persistent identity: range2017249
display id: range2017249
version: 1
direction: direction/reverse
top level: BBa_K196004
start: 940
end: 1716
orientation: inline

Sp1B
type: ComponentDefinition
persistent identity: BBa J176037
display id: BBa_J176037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sp1B
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: activation domain B from SP1; Emamai et al. 1995
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176037_sequence/1

BBa_K132000
type: ComponentDefinition
persistent identity: BBa K132000
display id: BBa_K132000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+kanR+LVA
creator: Du Jin,Hou Nan,Liu Duo,Yuan Fang,Zhang Weiran
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _219_
m user id: 0
owner id: 2627
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: true
mutable description: coding the kanamycin resistance gene with a LVA tag,so the protein can degrade in a relative short time
mutable notes: link the sequence with the standard scar in iGEM
mutable provenance: GENEART
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: LVA
sequence annotation: scar
sequence annotation: stop
sequence annotation: RBS
sequence annotation: kanR gene
sequence: https://synbiohub.org/public/igem/BBa_K132000_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1988218
display id: annotation1988218
version: 1
top level: BBa_K132000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K132000/annotation1988218/range1988218/1

https://synbiohub.org/public/igem/BBa_K132000/annotation1988218/range1988218/1
type: Range
persistent identity: range1988218
display id: range1988218
version: 1
direction: direction/forward
top level: BBa_K132000
start: 832
end: 864
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation1988215
display id: annotation1988215
version: 1
top level: BBa_K132000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K132000/annotation1988215/range1988215/1

https://synbiohub.org/public/igem/BBa_K132000/annotation1988215/range1988215/1
type: Range
persistent identity: range1988215
display id: range1988215
version: 1
direction: direction/forward
top level: BBa_K132000
start: 13
end: 18
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1988219
display id: annotation1988219
version: 1
top level: BBa_K132000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K132000/annotation1988219/range1988219/1

https://synbiohub.org/public/igem/BBa_K132000/annotation1988219/range1988219/1
type: Range
persistent identity: range1988219
display id: range1988219
version: 1
direction: direction/forward
top level: BBa_K132000
start: 865
end: 870
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1987900
display id: annotation1987900
version: 1
top level: BBa_K132000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K132000/annotation1987900/range1987900/1

https://synbiohub.org/public/igem/BBa_K132000/annotation1987900/range1987900/1
type: Range
persistent identity: range1987900
display id: range1987900
version: 1
direction: direction/forward
top level: BBa_K132000
start: 1
end: 12
orientation: inline

kanR gene
type: SequenceAnnotation
persistent identity: annotation1988217
display id: annotation1988217
version: 1
top level: BBa_K132000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K132000/annotation1988217/range1988217/1

https://synbiohub.org/public/igem/BBa_K132000/annotation1988217/range1988217/1
type: Range
persistent identity: range1988217
display id: range1988217
version: 1
direction: direction/forward
top level: BBa_K132000
start: 18
end: 831
orientation: inline

BBa_K731400
type: ComponentDefinition
persistent identity: BBa K731400
display id: BBa_K731400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Cysteine desulfhydrase (CysDes)
creator: Andrea Tassinari
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11988
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part was designed to construct a working CysDes gene under the control of an inducible promoter: the presence of IPTG inhibits the LacI repressor protein binding to the LacO operator; in this way it allows the RNApol to bind to the Ptac promoter and trascribe the gene of interest. In this case the gene of interest is a Cysteine Desulfhydrase, an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide.
mutable notes: LacI+LacIq promoter is an autonomous part (K731300) also designed by our team.
mutable provenance: LacI+LacIq promoter comes from pET-21b plasmid. CysDes gene comes from the genome of Treponema denticola.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K731300
component: BBa_K731600
sequence annotation: BBa_K731600
sequence annotation: BBa_K731300
sequence: https://synbiohub.org/public/igem/BBa_K731400_sequence/1

BBa_K731300
type: Component
persistent identity: component2180994
display id: component2180994
version: 1
top level: BBa_K731400
access: public
definition: https://synbiohub.org/public/igem/BBa_K731300/1

BBa_K731600
type: Component
persistent identity: component2181001
display id: component2181001
version: 1
top level: BBa_K731400
access: public
definition: https://synbiohub.org/public/igem/BBa_K731600/1

BBa_K731600
type: SequenceAnnotation
persistent identity: annotation2181001
display id: annotation2181001
version: 1
top level: BBa_K731400
location: https://synbiohub.org/public/igem/BBa_K731400/annotation2181001/range2181001/1
component: BBa_K731600

https://synbiohub.org/public/igem/BBa_K731400/annotation2181001/range2181001/1
type: Range
persistent identity: range2181001
display id: range2181001
version: 1
direction: direction/reverse
top level: BBa_K731400
start: 1162
end: 2466
orientation: inline

BBa_K731300
type: SequenceAnnotation
persistent identity: annotation2180994
display id: annotation2180994
version: 1
top level: BBa_K731400
location: https://synbiohub.org/public/igem/BBa_K731400/annotation2180994/range2180994/1
component: BBa_K731300

https://synbiohub.org/public/igem/BBa_K731400/annotation2180994/range2180994/1
type: Range
persistent identity: range2180994
display id: range2180994
version: 1
direction: direction/reverse
top level: BBa_K731400
start: 1
end: 1153
orientation: inline

BBa_K141003
type: ComponentDefinition
persistent identity: BBa K141003
display id: BBa_K141003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ucp 76 deleted
creator: Jaume For??s Martos
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _229_
m user id: 0
owner id: 2947
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K141003_sequence/1

LacI-T
type: ComponentDefinition
persistent identity: BBa K143062
display id: BBa_K143062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressor protein - Terminator
creator: Chris Hirst
created: 2008-09-30T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacI transcriptional repressor protein ( BBa_K143033 ) coupled to the double terminator ( BBa_B0015 . The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with ''B. subtilis''. LacI can be used in conjunction with the lac operon promoter ( BBa_K143015 ), where the LacI will act as a receiver for an IPTG input to result in a '''Polymerases per second''' (PoPS) output. The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012 . The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.
mutable notes: LacI was identified from the pDR111 ''B. subtilis'' integration vector. The double terminator is the most commonly used registry terminator.
mutable provenance: LacI was produced by PCR cloning using Pfu form the ''B. subtilis'' integration vector and cloned into a BioBrick with the registry double terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0012
component: BBa_K143033
sequence annotation: BBa_B0012
sequence annotation: BBa_K143033
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K143062_sequence/1

BBa_B0010
type: Component
persistent identity: component1994510
display id: component1994510
version: 1
top level: LacI-T
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1994512
display id: component1994512
version: 1
top level: LacI-T
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K143033
type: Component
persistent identity: component1994509
display id: component1994509
version: 1
top level: LacI-T
access: public
definition: LacI

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1994512
display id: annotation1994512
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994512/range1994512/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143062/annotation1994512/range1994512/1
type: Range
persistent identity: range1994512
display id: range1994512
version: 1
direction: direction/forward
top level: LacI-T
start: 1183
end: 1223
orientation: inline

BBa_K143033
type: SequenceAnnotation
persistent identity: annotation1994509
display id: annotation1994509
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994509/range1994509/1
component: BBa_K143033

https://synbiohub.org/public/igem/BBa_K143062/annotation1994509/range1994509/1
type: Range
persistent identity: range1994509
display id: range1994509
version: 1
direction: direction/reverse
top level: LacI-T
start: 1
end: 1086
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1994510
display id: annotation1994510
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994510/range1994510/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143062/annotation1994510/range1994510/1
type: Range
persistent identity: range1994510
display id: range1994510
version: 1
direction: direction/forward
top level: LacI-T
start: 1095
end: 1174
orientation: inline

BBa_K322124
type: ComponentDefinition
persistent identity: BBa K322124
display id: BBa_K322124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red light sensor Cph8 (from Coliroid) with RBS
creator: Chris French and Richard Edward Partridge-Hicks
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Cph8 is a combination of cph1 and envZ which, together with PCB, form a transmembrane, histidine kinase-based light sensing system. It contains the photoreceptor from Cph1 and the histidine kinase and response-regulator from EnvZ???OmpR, producing a particularly strongresponse to light. Features: cph8 coding sequence 16 to 2250, RBS 6 to 10.
mutable notes: Notes: we were unable to revive BioBrick I15010 with the coding sequence of the cph8 red light sensor fusion protein, so we sought to amplify it from BioBrick BBa_M30109. This led to numerous problems. It is clear from the Registry sequence data that M30109 is not what it is supposed to be. It does include cph8 with a ribosome binding site, but the RBS is not joined to cph8 by a standard scar; promoter R0040 may be present, but there appears to be an EcoRI site a little upstream of the ribosome binding site so that, when we cloned our PCR product, we failed to recover the promoter region; also the PstI site in cph8, which was mutated out in I15010, is still present in the version in M30109. We therefore performed corrective PCR to obtain a clone with the correct prefix, a ribosome binding site, and the cph8 coding sequence with the PstI site properly removed, which we present here, cloned in pSB1C3.
mutable provenance: BBa_I15010 (Photosensing Chimaera (Cph8) (not working, but amplified out of the whole construct))
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Cph8
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K322124_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation2083482
display id: annotation2083482
version: 1
top level: BBa_K322124
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K322124/annotation2083482/range2083482/1

https://synbiohub.org/public/igem/BBa_K322124/annotation2083482/range2083482/1
type: Range
persistent identity: range2083482
display id: range2083482
version: 1
direction: direction/forward
top level: BBa_K322124
start: 16
end: 2250
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2083481
display id: annotation2083481
version: 1
top level: BBa_K322124
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K322124/annotation2083481/range2083481/1

https://synbiohub.org/public/igem/BBa_K322124/annotation2083481/range2083481/1
type: Range
persistent identity: range2083481
display id: range2083481
version: 1
direction: direction/forward
top level: BBa_K322124
start: 6
end: 10
orientation: inline

BBa_K249001
type: ComponentDefinition
persistent identity: BBa K249001
display id: BBa_K249001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose Induced Inverter
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabinose Promoter with Tetracycline Inverter
mutable notes: Standard
mutable provenance: Existing BioBrick Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_I13453
component: BBa_Q04400
sequence annotation: BBa_Q04400
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K249001_sequence/1

BBa_I13453
type: Component
persistent identity: component2262207
display id: component2262207
version: 1
top level: BBa_K249001
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_Q04400
type: Component
persistent identity: component2262225
display id: component2262225
version: 1
top level: BBa_K249001
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04400/1

BBa_Q04400
type: SequenceAnnotation
persistent identity: annotation2262225
display id: annotation2262225
version: 1
top level: BBa_K249001
location: https://synbiohub.org/public/igem/BBa_K249001/annotation2262225/range2262225/1
component: BBa_Q04400

https://synbiohub.org/public/igem/BBa_K249001/annotation2262225/range2262225/1
type: Range
persistent identity: range2262225
display id: range2262225
version: 1
direction: direction/reverse
top level: BBa_K249001
start: 139
end: 1040
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2262207
display id: annotation2262207
version: 1
top level: BBa_K249001
location: https://synbiohub.org/public/igem/BBa_K249001/annotation2262207/range2262207/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K249001/annotation2262207/range2262207/1
type: Range
persistent identity: range2262207
display id: range2262207
version: 1
direction: direction/reverse
top level: BBa_K249001
start: 1
end: 130
orientation: inline

BBa_K249007
type: ComponentDefinition
persistent identity: BBa K249007
display id: BBa_K249007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP with C-terminal Arganine Fusion Tag
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: true
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This Cyan Fluorescent protein has been fused with 10 C-terminal Arginines to target it into the Lumazine-based microcompartment. Has a Ribosomal Binding Site
mutable notes: Standard
mutable provenance: BioBrick CFP with Synthesized C-terminal Arg Tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K249007_sequence/1

amilGFP
type: ComponentDefinition
persistent identity: BBa K592010
display id: BBa_K592010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilGFP, yellow chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein, amilGFP, naturally exhibits very strong yellow color when expressed. The color is strong and readily visible to naked eye both in LB-culture and on agar plates. The DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI).
mutable notes: Two illegal internal restriction sites (EcoRI and PstI) was removed.
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amilGFP
sequence: https://synbiohub.org/public/igem/BBa_K592010_sequence/1

amilGFP
type: SequenceAnnotation
persistent identity: annotation2131633
display id: annotation2131633
version: 1
top level: amilGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592010/annotation2131633/range2131633/1

https://synbiohub.org/public/igem/BBa_K592010/annotation2131633/range2131633/1
type: Range
persistent identity: range2131633
display id: range2131633
version: 1
direction: direction/forward
top level: amilGFP
start: 1
end: 696
orientation: inline

BBa_P10100
type: ComponentDefinition
persistent identity: BBa P10100
display id: BBa_P10100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s cauliflower mosaic virus + omega tobacco mosaic virus
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:48:17Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP12008
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10100_sequence/1

BBa_K1129050
type: ComponentDefinition
persistent identity: BBa K1129050
display id: BBa_K1129050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cinnamoyl-CoA reductase from Arabidopsis thaliana under arabinose promoter
creator: UBC iGEM 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1441_
m user id: 0
owner id: 9774
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arab.+rbs+AtCCR1
mutable notes: Arab.+rbs+AtCCR1
mutable provenance: genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_I13453
component: BBa_K1129005
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_K1129005
sequence: https://synbiohub.org/public/igem/BBa_K1129050_sequence/1

BBa_B0034
type: Component
persistent identity: component2353713
display id: component2353713
version: 1
top level: BBa_K1129050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13453
type: Component
persistent identity: component2353711
display id: component2353711
version: 1
top level: BBa_K1129050
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K1129005
type: Component
persistent identity: component2353714
display id: component2353714
version: 1
top level: BBa_K1129050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1129005/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2353711
display id: annotation2353711
version: 1
top level: BBa_K1129050
location: https://synbiohub.org/public/igem/BBa_K1129050/annotation2353711/range2353711/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1129050/annotation2353711/range2353711/1
type: Range
persistent identity: range2353711
display id: range2353711
version: 1
direction: direction/reverse
top level: BBa_K1129050
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2353713
display id: annotation2353713
version: 1
top level: BBa_K1129050
location: https://synbiohub.org/public/igem/BBa_K1129050/annotation2353713/range2353713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1129050/annotation2353713/range2353713/1
type: Range
persistent identity: range2353713
display id: range2353713
version: 1
direction: direction/reverse
top level: BBa_K1129050
start: 139
end: 150
orientation: inline

BBa_K1129005
type: SequenceAnnotation
persistent identity: annotation2353714
display id: annotation2353714
version: 1
top level: BBa_K1129050
location: https://synbiohub.org/public/igem/BBa_K1129050/annotation2353714/range2353714/1
component: BBa_K1129005

https://synbiohub.org/public/igem/BBa_K1129050/annotation2353714/range2353714/1
type: Range
persistent identity: range2353714
display id: range2353714
version: 1
direction: direction/reverse
top level: BBa_K1129050
start: 157
end: 1191
orientation: inline

BBa_K322123
type: ComponentDefinition
persistent identity: BBa K322123
display id: BBa_K322123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phycobilin synthesis operon without terminator
creator: Chris French and Richard Edward Partridge-Hicks
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor.
mutable notes: The actual forward sequence has the same changes at the start as seen in clone H11 described above. The reverse sequence is as expected. Features same as for the one above apart from the terminator.
mutable provenance: This is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: J23110
sequence annotation: pcyA
sequence annotation: B0032
sequence annotation: Heme oxygenase
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K322123_sequence/1

J23110
type: SequenceAnnotation
persistent identity: annotation2083043
display id: annotation2083043
version: 1
top level: BBa_K322123
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083043/range2083043/1

https://synbiohub.org/public/igem/BBa_K322123/annotation2083043/range2083043/1
type: Range
persistent identity: range2083043
display id: range2083043
version: 1
direction: direction/forward
top level: BBa_K322123
start: 1
end: 35
orientation: inline

pcyA
type: SequenceAnnotation
persistent identity: annotation2083047
display id: annotation2083047
version: 1
top level: BBa_K322123
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083047/range2083047/1

https://synbiohub.org/public/igem/BBa_K322123/annotation2083047/range2083047/1
type: Range
persistent identity: range2083047
display id: range2083047
version: 1
direction: direction/forward
top level: BBa_K322123
start: 650
end: 1396
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2083044
display id: annotation2083044
version: 1
top level: BBa_K322123
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083044/range2083044/1

https://synbiohub.org/public/igem/BBa_K322123/annotation2083044/range2083044/1
type: Range
persistent identity: range2083044
display id: range2083044
version: 1
direction: direction/forward
top level: BBa_K322123
start: 43
end: 54
orientation: inline

Heme oxygenase
type: SequenceAnnotation
persistent identity: annotation2083045
display id: annotation2083045
version: 1
top level: BBa_K322123
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083045/range2083045/1

https://synbiohub.org/public/igem/BBa_K322123/annotation2083045/range2083045/1
type: Range
persistent identity: range2083045
display id: range2083045
version: 1
direction: direction/forward
top level: BBa_K322123
start: 63
end: 629
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2083046
display id: annotation2083046
version: 1
top level: BBa_K322123
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083046/range2083046/1

https://synbiohub.org/public/igem/BBa_K322123/annotation2083046/range2083046/1
type: Range
persistent identity: range2083046
display id: range2083046
version: 1
direction: direction/forward
top level: BBa_K322123
start: 630
end: 644
orientation: inline

mCerulean
type: ComponentDefinition
persistent identity: BBa J18930
display id: BBa_J18930
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCerulean CFP
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cerulean GFP is an optimized FRET donor molecule that was rationally designed by Piston and co-workers to eliminate the excited-state heterogeneity of ECFP. In ECFP, a single-exponential fit to the fluorescence lifetime is not feasible due to multiple components, whereas Cerulean exhibits essentially homogeneous excited-state decay kinetics, rendering this protein useful for lifetime imaging... Live cell imaging experiments have demonstrated that Cerulean GFP undergoes highly efficient FRET to yellow acceptor molecules (25, 26). Additionally, Cerulean exhibits more than twice the brightness of ECFP and CyPet, emitting light at fluorescence intensities similar to Citrine (20). ===References=== * Rizzo MA, Springer GH, Granada B, Piston DW. (2004) An improved cyan fluorescent protein variant useful for FRET. PMID: 14990965
mutable notes: * amino acid sequence taken from a pAG304GAL-Cerulean-ccdB from LindQuist lab * borders trimmed to match the construct used for PDB 2Q57 (last 9 AA are not resolved in PDB) * structure starts with MSK, this construct starts with MVSK but the M has been skipped for this Biobrick * gene synthesis * codon optimized for E. coli
mutable provenance: literature gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18930_sequence/1

BBa_K208001
type: ComponentDefinition
persistent identity: BBa K208001
display id: BBa_K208001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phasin (PhaP)
creator: Elisabeth Linton
created: 2009-10-07T11:00:00Z
modified: 2015-05-08T01:11:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 2425
owning group id: 303
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: G
mutable notes: G
mutable provenance: G
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Phasin
sequence: https://synbiohub.org/public/igem/BBa_K208001_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2062218
display id: annotation2062218
version: 1
top level: BBa_K208001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K208001/annotation2062218/range2062218/1

https://synbiohub.org/public/igem/BBa_K208001/annotation2062218/range2062218/1
type: Range
persistent identity: range2062218
display id: range2062218
version: 1
direction: direction/forward
top level: BBa_K208001
start: 1
end: 3
orientation: inline

Phasin
type: SequenceAnnotation
persistent identity: annotation2062219
display id: annotation2062219
version: 1
top level: BBa_K208001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K208001/annotation2062219/range2062219/1

https://synbiohub.org/public/igem/BBa_K208001/annotation2062219/range2062219/1
type: Range
persistent identity: range2062219
display id: range2062219
version: 1
direction: direction/forward
top level: BBa_K208001
start: 1
end: 579
orientation: inline

BBa_K531011
type: ComponentDefinition
persistent identity: BBa K531011
display id: BBa_K531011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter and RBS BBa_K081005 + Caulobacter optimized esp + C-terminal sec
creator: Alexander Aaring
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
group u list: _697_
m user id: 0
owner id: 8840
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Produces and secretes active Esp with intact C-terminal secretion tag from RsaA. Esp is a suspected serine protease that breaks up Staphylococcus aureus biofilms.
mutable notes: Optimized esp does not have a stop codon to ensure expression of the fused secretion tag.
mutable provenance: BBa_081005 was submitted to the registry by 2008 iGEM Team UNIV_Pavia, codon optimized esp was synthesized by Integrated DNA Technologies, rsaA C-terminal secretion tag was copied from Caulobacter lab strain CB15N.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K531006
component: BBa_K531001
component: BBa_K081005
sequence annotation: BBa_K531006
sequence annotation: BBa_K081005
sequence annotation: BBa_K531001
sequence: https://synbiohub.org/public/igem/BBa_K531011_sequence/1

BBa_K531006
type: Component
persistent identity: component2146141
display id: component2146141
version: 1
top level: BBa_K531011
access: public
definition: BBa_K531006

BBa_K531001
type: Component
persistent identity: component2146142
display id: component2146142
version: 1
top level: BBa_K531011
access: public
definition: https://synbiohub.org/public/igem/BBa_K531001/1

BBa_K081005
type: Component
persistent identity: component2146140
display id: component2146140
version: 1
top level: BBa_K531011
access: public
definition: https://synbiohub.org/public/igem/BBa_K081005/1

BBa_K531006
type: SequenceAnnotation
persistent identity: annotation2146141
display id: annotation2146141
version: 1
top level: BBa_K531011
location: https://synbiohub.org/public/igem/BBa_K531011/annotation2146141/range2146141/1
component: BBa_K531006

https://synbiohub.org/public/igem/BBa_K531011/annotation2146141/range2146141/1
type: Range
persistent identity: range2146141
display id: range2146141
version: 1
direction: direction/reverse
top level: BBa_K531011
start: 67
end: 931
orientation: inline

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2146140
display id: annotation2146140
version: 1
top level: BBa_K531011
location: https://synbiohub.org/public/igem/BBa_K531011/annotation2146140/range2146140/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K531011/annotation2146140/range2146140/1
type: Range
persistent identity: range2146140
display id: range2146140
version: 1
direction: direction/reverse
top level: BBa_K531011
start: 1
end: 58
orientation: inline

BBa_K531001
type: SequenceAnnotation
persistent identity: annotation2146142
display id: annotation2146142
version: 1
top level: BBa_K531011
location: https://synbiohub.org/public/igem/BBa_K531011/annotation2146142/range2146142/1
component: BBa_K531001

https://synbiohub.org/public/igem/BBa_K531011/annotation2146142/range2146142/1
type: Range
persistent identity: range2146142
display id: range2146142
version: 1
direction: direction/reverse
top level: BBa_K531011
start: 940
end: 1299
orientation: inline

BBa_K1055000
type: ComponentDefinition
persistent identity: BBa K1055000
display id: BBa_K1055000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mKate is a fluorophor derived from dsRed
creator: Kai Fenzl
created: 2013-09-17T11:00:00Z
modified: 2015-05-08T01:08:57Z
discontinued: false
dominant: false
group u list: _1362_
m user id: 0
owner id: 16536
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: mKate is a fluorophor derived from dsRed. It features the excitation maxima at 588 nm and the emission maxima at 633 nm.
mutable notes: Nucleotide sequence was added in order to avoid the formation of secondary structures during gBLOCK assembly and subsequent steps. The amino acid sequence was left unchanged.
mutable provenance: IDT Technologies, gBLOCK assembly and subsequent PCR isolation
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence: https://synbiohub.org/public/igem/BBa_K1055000_sequence/1

BBa_K1031803
type: ComponentDefinition
persistent identity: BBa K1031803
display id: BBa_K1031803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu-B0031-sfGFP-Terminator (XylR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pu-31-sfGFP-TT (XylR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0031
sequence annotation: sfGFP
sequence annotation: Pu (XylR)
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031803_sequence/1

B0031
type: SequenceAnnotation
persistent identity: annotation2362526
display id: annotation2362526
version: 1
top level: BBa_K1031803
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362526/range2362526/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362526/range2362526/1
type: Range
persistent identity: range2362526
display id: range2362526
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 187
end: 200
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362527
display id: annotation2362527
version: 1
top level: BBa_K1031803
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362527/range2362527/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362527/range2362527/1
type: Range
persistent identity: range2362527
display id: range2362527
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 221
end: 929
orientation: inline

Pu (XylR)
type: SequenceAnnotation
persistent identity: annotation2362525
display id: annotation2362525
version: 1
top level: BBa_K1031803
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362525/range2362525/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362525/range2362525/1
type: Range
persistent identity: range2362525
display id: range2362525
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 1
end: 180
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362528
display id: annotation2362528
version: 1
top level: BBa_K1031803
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362528/range2362528/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362528/range2362528/1
type: Range
persistent identity: range2362528
display id: range2362528
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 936
end: 1064
orientation: inline

BBa_J176006
type: ComponentDefinition
persistent identity: BBa J176006
display id: BBa_J176006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP
creator: Brady Laughlin
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: Mammalian venus fluorescent protein
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J176006_sequence/1

BBa_K133046
type: ComponentDefinition
persistent identity: BBa K133046
display id: BBa_K133046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CF215
creator: Ana Lasic
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a part that is composed of C-terminal end of chimeric flagellin (BBa_K133016). We used aminoacids from 215th aminoacid on. Chimeric flagellin is composed of C- and N-terminal part of E.coli flagellin and of variable region of H.pylori. The E.coli parts are responsible for binding and activation of cell membrane receptor TLR5 to initiate immune response, while H.pylori variable region was used to array the immune response against H.pylori. This part was used in combination with CF213 - chimeric flagellin (BBa_K133010). Between the CF213 and CF215 we have put H.pylori antigens in order to array the immune response against H.pylori.
mutable notes: It was important to split the chimeric flagellin in the variable region to assure that the antigen will be in the right place and not to interrupt the 3d structure of the protein.
mutable provenance: We used polimerase chain reaction from chimeric flagellin (BBa_K133016).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133046_sequence/1

BBa_K554006
type: ComponentDefinition
persistent identity: BBa K554006
display id: BBa_K554006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QseB and QseC
creator: UNICAMP EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: QseB and QseC is ...
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: RBS
sequence annotation: QseB
sequence annotation: QseC
sequence: https://synbiohub.org/public/igem/BBa_K554006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2136908
display id: annotation2136908
version: 1
top level: BBa_K554006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K554006/annotation2136908/range2136908/1

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type: Range
persistent identity: range2136908
display id: range2136908
version: 1
direction: direction/forward
top level: BBa_K554006
start: 2041
end: 2046
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2136903
display id: annotation2136903
version: 1
top level: BBa_K554006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K554006/annotation2136903/range2136903/1

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type: Range
persistent identity: range2136903
display id: range2136903
version: 1
direction: direction/forward
top level: BBa_K554006
start: 673
end: 678
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136906
display id: annotation2136906
version: 1
top level: BBa_K554006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K554006/annotation2136906/range2136906/1

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type: Range
persistent identity: range2136906
display id: range2136906
version: 1
direction: direction/forward
top level: BBa_K554006
start: 679
end: 693
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136901
display id: annotation2136901
version: 1
top level: BBa_K554006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K554006/annotation2136901/range2136901/1

https://synbiohub.org/public/igem/BBa_K554006/annotation2136901/range2136901/1
type: Range
persistent identity: range2136901
display id: range2136901
version: 1
direction: direction/forward
top level: BBa_K554006
start: 1
end: 15
orientation: inline

QseB
type: SequenceAnnotation
persistent identity: annotation2136902
display id: annotation2136902
version: 1
top level: BBa_K554006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K554006/annotation2136902/range2136902/1

https://synbiohub.org/public/igem/BBa_K554006/annotation2136902/range2136902/1
type: Range
persistent identity: range2136902
display id: range2136902
version: 1
direction: direction/forward
top level: BBa_K554006
start: 16
end: 672
orientation: inline

QseC
type: SequenceAnnotation
persistent identity: annotation2136907
display id: annotation2136907
version: 1
top level: BBa_K554006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K554006/annotation2136907/range2136907/1

https://synbiohub.org/public/igem/BBa_K554006/annotation2136907/range2136907/1
type: Range
persistent identity: range2136907
display id: range2136907
version: 1
direction: direction/forward
top level: BBa_K554006
start: 694
end: 2040
orientation: inline

BBa_K1189007
type: ComponentDefinition
persistent identity: BBa K1189007
display id: BBa_K1189007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-lactamase with His Tag under the control of the inducible lacI promoter
creator: Taylor Remondini
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1502_
m user id: 0
owner id: 13453
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: dasdsa
mutable notes: dsadas
mutable provenance: adasd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1189009
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K1189009
sequence: https://synbiohub.org/public/igem/BBa_K1189007_sequence/1

BBa_K1189009
type: Component
persistent identity: component2366024
display id: component2366024
version: 1
top level: BBa_K1189007
access: public
definition: BBa_K1189009

BBa_J04500
type: Component
persistent identity: component2366019
display id: component2366019
version: 1
top level: BBa_K1189007
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2366019
display id: annotation2366019
version: 1
top level: BBa_K1189007
location: https://synbiohub.org/public/igem/BBa_K1189007/annotation2366019/range2366019/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1189007/annotation2366019/range2366019/1
type: Range
persistent identity: range2366019
display id: range2366019
version: 1
direction: direction/reverse
top level: BBa_K1189007
start: 1
end: 220
orientation: inline

BBa_K1189009
type: SequenceAnnotation
persistent identity: annotation2366024
display id: annotation2366024
version: 1
top level: BBa_K1189007
location: https://synbiohub.org/public/igem/BBa_K1189007/annotation2366024/range2366024/1
component: BBa_K1189009

https://synbiohub.org/public/igem/BBa_K1189007/annotation2366024/range2366024/1
type: Range
persistent identity: range2366024
display id: range2366024
version: 1
direction: direction/reverse
top level: BBa_K1189007
start: 229
end: 1112
orientation: inline

BBa_K215002
type: ComponentDefinition
persistent identity: BBa K215002
display id: BBa_K215002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac+RBS+Secretion Signal and Streptavidin Binding Tags
creator: Jeff Nivala
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: nnn
mutable notes: nnn
mutable provenance: nnn
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K215001
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_K215001
sequence: https://synbiohub.org/public/igem/BBa_K215002_sequence/1

BBa_B0034
type: Component
persistent identity: component2027627
display id: component2027627
version: 1
top level: BBa_K215002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K215001
type: Component
persistent identity: component2027642
display id: component2027642
version: 1
top level: BBa_K215002
access: public
definition: BBa_K215001

BBa_R0011
type: Component
persistent identity: component2027621
display id: component2027621
version: 1
top level: BBa_K215002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2027621
display id: annotation2027621
version: 1
top level: BBa_K215002
location: https://synbiohub.org/public/igem/BBa_K215002/annotation2027621/range2027621/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K215002/annotation2027621/range2027621/1
type: Range
persistent identity: range2027621
display id: range2027621
version: 1
direction: direction/forward
top level: BBa_K215002
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2027627
display id: annotation2027627
version: 1
top level: BBa_K215002
location: https://synbiohub.org/public/igem/BBa_K215002/annotation2027627/range2027627/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K215002/annotation2027627/range2027627/1
type: Range
persistent identity: range2027627
display id: range2027627
version: 1
direction: direction/reverse
top level: BBa_K215002
start: 64
end: 75
orientation: inline

BBa_K215001
type: SequenceAnnotation
persistent identity: annotation2027642
display id: annotation2027642
version: 1
top level: BBa_K215002
location: https://synbiohub.org/public/igem/BBa_K215002/annotation2027642/range2027642/1
component: BBa_K215001

https://synbiohub.org/public/igem/BBa_K215002/annotation2027642/range2027642/1
type: Range
persistent identity: range2027642
display id: range2027642
version: 1
direction: direction/reverse
top level: BBa_K215002
start: 82
end: 946
orientation: inline

BBa_K274381
type: ComponentDefinition
persistent identity: BBa K274381
display id: BBa_K274381
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> PSP3 pag - PO promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746361
component: BBa_B0012
component: BBa_B0010
component: BBa_I746351
sequence annotation: BBa_B0012
sequence annotation: BBa_I746361
sequence annotation: BBa_B0010
sequence annotation: BBa_I746351
sequence: https://synbiohub.org/public/igem/BBa_K274381_sequence/1

BBa_I746361
type: Component
persistent identity: component2055350
display id: component2055350
version: 1
top level: BBa_K274381
access: public
definition: https://synbiohub.org/public/igem/BBa_I746361/1

BBa_B0012
type: Component
persistent identity: component2055345
display id: component2055345
version: 1
top level: BBa_K274381
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2055343
display id: component2055343
version: 1
top level: BBa_K274381
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746351
type: Component
persistent identity: component2055342
display id: component2055342
version: 1
top level: BBa_K274381
access: public
definition: https://synbiohub.org/public/igem/BBa_I746351/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055345
display id: annotation2055345
version: 1
top level: BBa_K274381
location: https://synbiohub.org/public/igem/BBa_K274381/annotation2055345/range2055345/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274381/annotation2055345/range2055345/1
type: Range
persistent identity: range2055345
display id: range2055345
version: 1
direction: direction/forward
top level: BBa_K274381
start: 334
end: 374
orientation: inline

BBa_I746361
type: SequenceAnnotation
persistent identity: annotation2055350
display id: annotation2055350
version: 1
top level: BBa_K274381
location: https://synbiohub.org/public/igem/BBa_K274381/annotation2055350/range2055350/1
component: BBa_I746361

https://synbiohub.org/public/igem/BBa_K274381/annotation2055350/range2055350/1
type: Range
persistent identity: range2055350
display id: range2055350
version: 1
direction: direction/reverse
top level: BBa_K274381
start: 383
end: 474
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055343
display id: annotation2055343
version: 1
top level: BBa_K274381
location: https://synbiohub.org/public/igem/BBa_K274381/annotation2055343/range2055343/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274381/annotation2055343/range2055343/1
type: Range
persistent identity: range2055343
display id: range2055343
version: 1
direction: direction/forward
top level: BBa_K274381
start: 246
end: 325
orientation: inline

BBa_I746351
type: SequenceAnnotation
persistent identity: annotation2055342
display id: annotation2055342
version: 1
top level: BBa_K274381
location: https://synbiohub.org/public/igem/BBa_K274381/annotation2055342/range2055342/1
component: BBa_I746351

https://synbiohub.org/public/igem/BBa_K274381/annotation2055342/range2055342/1
type: Range
persistent identity: range2055342
display id: range2055342
version: 1
direction: direction/reverse
top level: BBa_K274381
start: 1
end: 237
orientation: inline

clyA_Nter
type: ComponentDefinition
persistent identity: BBa K257007
display id: BBa_K257007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ClyA_Fusion_N-Term
creator: Guillaume Cambray
created: 2009-09-09T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _354_
m user id: 0
owner id: 5366
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ClyA is exported to the periplasm and exposed in outer-membrane vesicles
mutable notes: For fusion of proteins to the N-terminal extremity of ClyA using the silver fusion standard RFC23
mutable provenance: E. coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: hlyE
sequence annotation: poly GLY linker
sequence annotation: double codon stop
sequence: https://synbiohub.org/public/igem/BBa_K257007_sequence/1

hlyE
type: SequenceAnnotation
persistent identity: annotation2057161
display id: annotation2057161
version: 1
top level: clyA_Nter
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K257007/annotation2057161/range2057161/1

https://synbiohub.org/public/igem/BBa_K257007/annotation2057161/range2057161/1
type: Range
persistent identity: range2057161
display id: range2057161
version: 1
direction: direction/forward
top level: clyA_Nter
start: 16
end: 921
orientation: inline

poly GLY linker
type: SequenceAnnotation
persistent identity: annotation2020630
display id: annotation2020630
version: 1
top level: clyA_Nter
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K257007/annotation2020630/range2020630/1

https://synbiohub.org/public/igem/BBa_K257007/annotation2020630/range2020630/1
type: Range
persistent identity: range2020630
display id: range2020630
version: 1
direction: direction/forward
top level: clyA_Nter
start: 1
end: 15
orientation: inline

double codon stop
type: SequenceAnnotation
persistent identity: annotation2057169
display id: annotation2057169
version: 1
top level: clyA_Nter
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K257007/annotation2057169/range2057169/1

https://synbiohub.org/public/igem/BBa_K257007/annotation2057169/range2057169/1
type: Range
persistent identity: range2057169
display id: range2057169
version: 1
direction: direction/forward
top level: clyA_Nter
start: 922
end: 927
orientation: inline

BBa_K116401
type: ComponentDefinition
persistent identity: BBa K116401
display id: BBa_K116401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: external phosphate sensing promoter
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: promoter of phoB in E.coli, an external phosphate regulated promoter which can be activated when encountering phosphate limitation.
mutable notes: The promoter region should contain PhoB binding site
mutable provenance: E. coli K12 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: PhoB binding site
sequence: https://synbiohub.org/public/igem/BBa_K116401_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1995352
display id: annotation1995352
version: 1
top level: BBa_K116401
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995352/range1995352/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995352/range1995352/1
type: Range
persistent identity: range1995352
display id: range1995352
version: 1
direction: direction/forward
top level: BBa_K116401
start: 421
end: 429
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1995353
display id: annotation1995353
version: 1
top level: BBa_K116401
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995353/range1995353/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995353/range1995353/1
type: Range
persistent identity: range1995353
display id: range1995353
version: 1
direction: direction/forward
top level: BBa_K116401
start: 448
end: 455
orientation: inline

PhoB binding site
type: SequenceAnnotation
persistent identity: annotation1995351
display id: annotation1995351
version: 1
top level: BBa_K116401
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995351/range1995351/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995351/range1995351/1
type: Range
persistent identity: range1995351
display id: range1995351
version: 1
direction: direction/forward
top level: BBa_K116401
start: 420
end: 439
orientation: inline

BBa_M1600
type: ComponentDefinition
persistent identity: BBa M1600
display id: BBa_M1600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CXCR3
creator: Yoshitaro Heshiki
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12289
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: putative type III polyketide synthase (PKS) gene
mutable notes: na
mutable provenance: Aspergillus oryzae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Stop
sequence annotation: Coding Sequence
sequence: https://synbiohub.org/public/igem/BBa_M1600_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2171438
display id: annotation2171438
version: 1
top level: BBa_M1600
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M1600/annotation2171438/range2171438/1

https://synbiohub.org/public/igem/BBa_M1600/annotation2171438/range2171438/1
type: Range
persistent identity: range2171438
display id: range2171438
version: 1
direction: direction/forward
top level: BBa_M1600
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2171440
display id: annotation2171440
version: 1
top level: BBa_M1600
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M1600/annotation2171440/range2171440/1

https://synbiohub.org/public/igem/BBa_M1600/annotation2171440/range2171440/1
type: Range
persistent identity: range2171440
display id: range2171440
version: 1
direction: direction/forward
top level: BBa_M1600
start: 1099
end: 1101
orientation: inline

Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2171439
display id: annotation2171439
version: 1
top level: BBa_M1600
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M1600/annotation2171439/range2171439/1

https://synbiohub.org/public/igem/BBa_M1600/annotation2171439/range2171439/1
type: Range
persistent identity: range2171439
display id: range2171439
version: 1
direction: direction/forward
top level: BBa_M1600
start: 1
end: 1101
orientation: inline

BBa_K557001
type: ComponentDefinition
persistent identity: BBa K557001
display id: BBa_K557001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aptamer-CheZ: theophylline-sensitive synthetic riboswitch+phosphatase which dephosphorylates CheY-P
creator: Yinghong Lan
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is composed of a theophylline-sensitive aptamer,which will pair the ribosome binding site of the downstream gene in the absence of theophylline and thus inhibit the translation of the gene while expose the ribosome binding site in the presence of theophylline, and the CheZ gene, which is a phosphatase which dephosphorylates CheY-P and causes the flagellum to rotate counterclockwise and is essential for keeping the motility of E.coli. Thus this part provides a ligand-inducible motility and makes it possible to guide E.coli by theophylline.
mutable notes: We found the sequence of this part in literature and tried to design primers to construct it. But we were faced with a challenging problem in primer design, which comes from the basic properties of the aptamer involved. The aptamer tends to pair the ribosome binding site in the absence of theophylline, which is true in PCR, and enables primers to pair to the corresponding parts and the new strand to elongate. We made contact with the author of our reference and found they took different strategies in assembling the aptamer to CheZ instead of PCR. But thanks to the warm help of Professor J. S. Parkinson and Professor J. P. Gallivan, we acquired the sequence of primers used in assembling the aptamer to another gene and designed our own after analyzing them: forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGGGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACCCCGCTGCAAGACAACAAG ATGCAACCATCAATCAAACC-3' reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTATTATTAAAATCCAAGACTATCCAACAAATCGT-3' Besides, we used special protocal of PCR: 96℃ 10min 96℃ 30s, 56℃ 30s, 72℃ 1min for 16 cycles and each cycle the annealing temperature increases by 1℃ 96℃ 30s, 72℃ 30s, 72℃ 1min for 20 cycles 72℃ 10min
mutable provenance: Escherichia coli, the strain has always been kept in the lab of USTC-IGEM
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: aptamer
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K557001_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2149327
display id: annotation2149327
version: 1
top level: BBa_K557001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149327/range2149327/1

https://synbiohub.org/public/igem/BBa_K557001/annotation2149327/range2149327/1
type: Range
persistent identity: range2149327
display id: range2149327
version: 1
direction: direction/forward
top level: BBa_K557001
start: 47
end: 57
orientation: inline

aptamer
type: SequenceAnnotation
persistent identity: annotation2149326
display id: annotation2149326
version: 1
top level: BBa_K557001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149326/range2149326/1

https://synbiohub.org/public/igem/BBa_K557001/annotation2149326/range2149326/1
type: Range
persistent identity: range2149326
display id: range2149326
version: 1
direction: direction/forward
top level: BBa_K557001
start: 1
end: 46
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2149328
display id: annotation2149328
version: 1
top level: BBa_K557001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149328/range2149328/1

https://synbiohub.org/public/igem/BBa_K557001/annotation2149328/range2149328/1
type: Range
persistent identity: range2149328
display id: range2149328
version: 1
direction: direction/forward
top level: BBa_K557001
start: 58
end: 60
orientation: inline

cI857
type: ComponentDefinition
persistent identity: BBa J06503
display id: BBa_J06503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI857 (Lambda II) (RBS- LVA+)
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mutated from BBa_C0051 Repressor, Lambda cI (RBS- LVA+) to be temperature sensitive and UV insensitive.
mutable notes: First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutate succeeded. However, the sequencing had a lot of bad sequence at the beginings, so that the ind- mutation was not sequenced by either of the primers used. The ind- mutation was verified by the presence of a HindIII restrition site. This part was never completely sequenced. The cI857 QPI was made by Blue Heron, and this was part was abandoned.
mutable provenance: Hendrix, Roger W. et al., ed. Lambda II. New York: Cold Spring Harbor Laboratory, 1983. ind- mutation on p. 626 (at bp 37589 of Lambda) 857 mutation on p. 628 (at bp 37742 of Lambda) Made by introducing two point mutations to Lambda cI (BBa_C0051): G(352)->A for ind- and G(199)->A for 857 temperature sensitivity.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G->A (857)
sequence annotation: G->A (ind-)
sequence annotation: cI857
sequence annotation: barcode from LacI-LVA (BBa_C0012)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J06503_sequence/1

G->A (857)
type: SequenceAnnotation
persistent identity: annotation1549980
display id: annotation1549980
version: 1
top level: cI857
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549980/range1549980/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1549980/range1549980/1
type: Range
persistent identity: range1549980
display id: range1549980
version: 1
direction: direction/forward
top level: cI857
start: 199
end: 199
orientation: inline

G->A (ind-)
type: SequenceAnnotation
persistent identity: annotation1549981
display id: annotation1549981
version: 1
top level: cI857
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549981/range1549981/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1549981/range1549981/1
type: Range
persistent identity: range1549981
display id: range1549981
version: 1
direction: direction/forward
top level: cI857
start: 352
end: 352
orientation: inline

cI857
type: SequenceAnnotation
persistent identity: annotation1549975
display id: annotation1549975
version: 1
top level: cI857
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549975/range1549975/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1549975/range1549975/1
type: Range
persistent identity: range1549975
display id: range1549975
version: 1
direction: direction/forward
top level: cI857
start: 1
end: 711
orientation: inline

barcode from LacI-LVA (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1552049
display id: annotation1552049
version: 1
top level: cI857
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1552049/range1552049/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1552049/range1552049/1
type: Range
persistent identity: range1552049
display id: range1552049
version: 1
direction: direction/forward
top level: cI857
start: 751
end: 775
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1549979
display id: annotation1549979
version: 1
top level: cI857
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549979/range1549979/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1549979/range1549979/1
type: Range
persistent identity: range1549979
display id: range1549979
version: 1
direction: direction/forward
top level: cI857
start: 712
end: 744
orientation: inline

CMV
type: ComponentDefinition
persistent identity: BBa J176027
display id: BBa_J176027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: constitute CMV promoter;PCR cloned from pcDNA3.1+
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J176027_sequence/1

eforRed
type: ComponentDefinition
persistent identity: BBa K592012
display id: BBa_K592012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed, red chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein eforRed naturally exhibits red color when expressed. The color is weaker than RFP, however. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
mutable notes: coming soon
mutable provenance: coming soon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: eforRed
sequence: https://synbiohub.org/public/igem/BBa_K592012_sequence/1

eforRed
type: SequenceAnnotation
persistent identity: annotation2131799
display id: annotation2131799
version: 1
top level: eforRed
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1

https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
type: Range
persistent identity: range2131799
display id: range2131799
version: 1
direction: direction/forward
top level: eforRed
start: 1
end: 681
orientation: inline

BBa_K801080
type: ComponentDefinition
persistent identity: BBa K801080
display id: BBa_K801080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prepro-Thaumatin, yeast codon optimized
creator: Alois Br??uer
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: Coding region for Prepro-Thaumatin. Codon optimized for yeast.
mutable notes: Synthesized by IDT. The part is codon optimized for yeast. 3 forbidden restriction sites have been removed.
mutable provenance: Based on: http://www.ncbi.nlm.nih.gov/nuccore/121945717
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Pre-signal sequence
sequence annotation: Pro-signal sequence
sequence annotation: Preprothaumatin
sequence: https://synbiohub.org/public/igem/BBa_K801080_sequence/1

Pre-signal sequence
type: SequenceAnnotation
persistent identity: annotation2343094
display id: annotation2343094
version: 1
top level: BBa_K801080
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343094/range2343094/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343094/range2343094/1
type: Range
persistent identity: range2343094
display id: range2343094
version: 1
direction: direction/forward
top level: BBa_K801080
start: 1
end: 67
orientation: inline

Pro-signal sequence
type: SequenceAnnotation
persistent identity: annotation2343095
display id: annotation2343095
version: 1
top level: BBa_K801080
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343095/range2343095/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343095/range2343095/1
type: Range
persistent identity: range2343095
display id: range2343095
version: 1
direction: direction/forward
top level: BBa_K801080
start: 688
end: 705
orientation: inline

Preprothaumatin
type: SequenceAnnotation
persistent identity: annotation2343093
display id: annotation2343093
version: 1
top level: BBa_K801080
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343093/range2343093/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343093/range2343093/1
type: Range
persistent identity: range2343093
display id: range2343093
version: 1
direction: direction/forward
top level: BBa_K801080
start: 1
end: 705
orientation: inline

BBa_M45995
type: ComponentDefinition
persistent identity: BBa M45995
display id: BBa_M45995
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phasin coding sequence
creator: Charles Miller
created: 2014-03-24T12:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1855_
m user id: 0
owner id: 2425
owning group id: 303
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: fdglkdjg;lfdskg
mutable notes: had remove resti
mutable provenance: fgjhds
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop Codon
sequence annotation: Coding Sequence
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_M45995_sequence/1

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2371898
display id: annotation2371898
version: 1
top level: BBa_M45995
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M45995/annotation2371898/range2371898/1

https://synbiohub.org/public/igem/BBa_M45995/annotation2371898/range2371898/1
type: Range
persistent identity: range2371898
display id: range2371898
version: 1
direction: direction/forward
top level: BBa_M45995
start: 684
end: 686
orientation: inline

Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2371889
display id: annotation2371889
version: 1
top level: BBa_M45995
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M45995/annotation2371889/range2371889/1

https://synbiohub.org/public/igem/BBa_M45995/annotation2371889/range2371889/1
type: Range
persistent identity: range2371889
display id: range2371889
version: 1
direction: direction/forward
top level: BBa_M45995
start: 1
end: 686
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2371892
display id: annotation2371892
version: 1
top level: BBa_M45995
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M45995/annotation2371892/range2371892/1

https://synbiohub.org/public/igem/BBa_M45995/annotation2371892/range2371892/1
type: Range
persistent identity: range2371892
display id: range2371892
version: 1
direction: direction/forward
top level: BBa_M45995
start: 1
end: 3
orientation: inline

BBa_K1031301
type: ComponentDefinition
persistent identity: BBa K1031301
display id: BBa_K1031301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc-B0031-sfGFP-Terminator (HbpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-31-sfGFP-TT (HbpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Ph
sequence annotation: BBa_B0031
sequence annotation: sfGFP
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031301_sequence/1

Ph
type: SequenceAnnotation
persistent identity: annotation2360426
display id: annotation2360426
version: 1
top level: BBa_K1031301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1
type: Range
persistent identity: range2360426
display id: range2360426
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 1
end: 229
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2360427
display id: annotation2360427
version: 1
top level: BBa_K1031301
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1
type: Range
persistent identity: range2360427
display id: range2360427
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 236
end: 248
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360428
display id: annotation2360428
version: 1
top level: BBa_K1031301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1
type: Range
persistent identity: range2360428
display id: range2360428
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 255
end: 977
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360429
display id: annotation2360429
version: 1
top level: BBa_K1031301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1
type: Range
persistent identity: range2360429
display id: range2360429
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 984
end: 1112
orientation: inline

BBa_K091225
type: ComponentDefinition
persistent identity: BBa K091225
display id: BBa_K091225
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD RBS LacI
creator: Xiao Zhu
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:08:39Z
discontinued: false
dominant: false
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: this is the intermediate construct combining pBAD with BBa_S0100, so as to express lacI gene
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I13453
component: BBa_S0100
sequence annotation: BBa_I13453
sequence annotation: BBa_S0100
sequence: https://synbiohub.org/public/igem/BBa_K091225_sequence/1

BBa_I13453
type: Component
persistent identity: component2246704
display id: component2246704
version: 1
top level: BBa_K091225
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_S0100
type: Component
persistent identity: component2246711
display id: component2246711
version: 1
top level: BBa_K091225
access: public
definition: https://synbiohub.org/public/igem/BBa_S0100/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2246704
display id: annotation2246704
version: 1
top level: BBa_K091225
location: https://synbiohub.org/public/igem/BBa_K091225/annotation2246704/range2246704/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K091225/annotation2246704/range2246704/1
type: Range
persistent identity: range2246704
display id: range2246704
version: 1
direction: direction/reverse
top level: BBa_K091225
start: 1
end: 130
orientation: inline

BBa_S0100
type: SequenceAnnotation
persistent identity: annotation2246711
display id: annotation2246711
version: 1
top level: BBa_K091225
location: https://synbiohub.org/public/igem/BBa_K091225/annotation2246711/range2246711/1
component: BBa_S0100

https://synbiohub.org/public/igem/BBa_K091225/annotation2246711/range2246711/1
type: Range
persistent identity: range2246711
display id: range2246711
version: 1
direction: direction/reverse
top level: BBa_K091225
start: 139
end: 1309
orientation: inline

BBa_P10302
type: ComponentDefinition
persistent identity: BBa P10302
display id: BBa_P10302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GVG
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:57:03Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP80020
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10302_sequence/1

BBa_K781001
type: ComponentDefinition
persistent identity: BBa K781001
display id: BBa_K781001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [R0010][B0034] - RFP-FliC Insertion Chimera
creator: Kevin Chen
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1035_
m user id: 0
owner id: 9715
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the modified RFP protein obtained from J04450, that has been incorporated into the E. coli flagellin as an insertion. This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies.
mutable notes: The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.
mutable provenance: This was created using overlap extension PCR to insert into K781000 at the annotated site. The insert sequence was amplified using PCR from J04450.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: conserved
sequence annotation: -35
sequence annotation: BBa_E1010
sequence annotation: Flagellin
sequence annotation: LacI
sequence annotation: 1st Overlapping region
sequence annotation: 2nd Overlapping region
sequence annotation: -10
sequence annotation: Linker
sequence annotation: Linker 2
sequence annotation: BBa_J04500
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: CAP
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K781001_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2211294
display id: annotation2211294
version: 1
top level: BBa_K781001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211294/range2211294/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211294/range2211294/1
type: Range
persistent identity: range2211294
display id: range2211294
version: 1
direction: direction/forward
top level: BBa_K781001
start: 213
end: 216
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2211289
display id: annotation2211289
version: 1
top level: BBa_K781001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211289/range2211289/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211289/range2211289/1
type: Range
persistent identity: range2211289
display id: range2211289
version: 1
direction: direction/forward
top level: BBa_K781001
start: 137
end: 142
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2211298
display id: annotation2211298
version: 1
top level: BBa_K781001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211298/range2211298/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211298/range2211298/1
type: Range
persistent identity: range2211298
display id: range2211298
version: 1
direction: direction/forward
top level: BBa_K781001
start: 997
end: 1672
orientation: inline

Flagellin
type: SequenceAnnotation
persistent identity: annotation2211300
display id: annotation2211300
version: 1
top level: BBa_K781001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211300/range2211300/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211300/range2211300/1
type: Range
persistent identity: range2211300
display id: range2211300
version: 1
direction: direction/forward
top level: BBa_K781001
start: 1693
end: 2443
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2211291
display id: annotation2211291
version: 1
top level: BBa_K781001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211291/range2211291/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211291/range2211291/1
type: Range
persistent identity: range2211291
display id: range2211291
version: 1
direction: direction/forward
top level: BBa_K781001
start: 166
end: 200
orientation: inline

1st Overlapping region
type: SequenceAnnotation
persistent identity: annotation2211296
display id: annotation2211296
version: 1
top level: BBa_K781001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211296/range2211296/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211296/range2211296/1
type: Range
persistent identity: range2211296
display id: range2211296
version: 1
direction: direction/reverse
top level: BBa_K781001
start: 946
end: 976
orientation: inline

2nd Overlapping region
type: SequenceAnnotation
persistent identity: annotation2211301
display id: annotation2211301
version: 1
top level: BBa_K781001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211301/range2211301/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211301/range2211301/1
type: Range
persistent identity: range2211301
display id: range2211301
version: 1
direction: direction/forward
top level: BBa_K781001
start: 1693
end: 1721
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2211290
display id: annotation2211290
version: 1
top level: BBa_K781001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211290/range2211290/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211290/range2211290/1
type: Range
persistent identity: range2211290
display id: range2211290
version: 1
direction: direction/forward
top level: BBa_K781001
start: 161
end: 166
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2211297
display id: annotation2211297
version: 1
top level: BBa_K781001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211297/range2211297/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211297/range2211297/1
type: Range
persistent identity: range2211297
display id: range2211297
version: 1
direction: direction/forward
top level: BBa_K781001
start: 977
end: 996
orientation: inline

Flagellin
type: SequenceAnnotation
persistent identity: annotation2211295
display id: annotation2211295
version: 1
top level: BBa_K781001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211295/range2211295/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211295/range2211295/1
type: Range
persistent identity: range2211295
display id: range2211295
version: 1
direction: direction/forward
top level: BBa_K781001
start: 230
end: 975
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2211299
display id: annotation2211299
version: 1
top level: BBa_K781001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211299/range2211299/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211299/range2211299/1
type: Range
persistent identity: range2211299
display id: range2211299
version: 1
direction: direction/forward
top level: BBa_K781001
start: 1673
end: 1692
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2211287
display id: annotation2211287
version: 1
top level: BBa_K781001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211287/range2211287/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211287/range2211287/1
type: Range
persistent identity: range2211287
display id: range2211287
version: 1
direction: direction/reverse
top level: BBa_K781001
start: 1
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2211286
display id: annotation2211286
version: 1
top level: BBa_K781001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211286/range2211286/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211286/range2211286/1
type: Range
persistent identity: range2211286
display id: range2211286
version: 1
direction: direction/forward
top level: BBa_K781001
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2211293
display id: annotation2211293
version: 1
top level: BBa_K781001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211293/range2211293/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211293/range2211293/1
type: Range
persistent identity: range2211293
display id: range2211293
version: 1
direction: direction/forward
top level: BBa_K781001
start: 209
end: 220
orientation: inline

CAP
type: SequenceAnnotation
persistent identity: annotation2211288
display id: annotation2211288
version: 1
top level: BBa_K781001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211288/range2211288/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211288/range2211288/1
type: Range
persistent identity: range2211288
display id: range2211288
version: 1
direction: direction/forward
top level: BBa_K781001
start: 89
end: 126
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2211292
display id: annotation2211292
version: 1
top level: BBa_K781001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K781001/annotation2211292/range2211292/1

https://synbiohub.org/public/igem/BBa_K781001/annotation2211292/range2211292/1
type: Range
persistent identity: range2211292
display id: range2211292
version: 1
direction: direction/forward
top level: BBa_K781001
start: 173
end: 173
orientation: inline

BBa_K133055
type: ComponentDefinition
persistent identity: BBa K133055
display id: BBa_K133055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry
creator: Ana Lasic
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: mCherry is a red fluorescent protein used for analysis of localisation of protein.
mutable notes: none
mutable provenance: we got it from
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K133055_sequence/1

BBa_K274370
type: ComponentDefinition
persistent identity: BBa K274370
display id: BBa_K274370
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> P2 ogr - PF promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_I746360
component: BBa_B0012
component: BBa_I746350
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I746350
sequence annotation: BBa_I746360
sequence: https://synbiohub.org/public/igem/BBa_K274370_sequence/1

BBa_B0010
type: Component
persistent identity: component2055283
display id: component2055283
version: 1
top level: BBa_K274370
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746360
type: Component
persistent identity: component2055290
display id: component2055290
version: 1
top level: BBa_K274370
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_B0012
type: Component
persistent identity: component2055285
display id: component2055285
version: 1
top level: BBa_K274370
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746350
type: Component
persistent identity: component2055282
display id: component2055282
version: 1
top level: BBa_K274370
access: public
definition: https://synbiohub.org/public/igem/BBa_I746350/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055285
display id: annotation2055285
version: 1
top level: BBa_K274370
location: https://synbiohub.org/public/igem/BBa_K274370/annotation2055285/range2055285/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274370/annotation2055285/range2055285/1
type: Range
persistent identity: range2055285
display id: range2055285
version: 1
direction: direction/forward
top level: BBa_K274370
start: 334
end: 374
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055283
display id: annotation2055283
version: 1
top level: BBa_K274370
location: https://synbiohub.org/public/igem/BBa_K274370/annotation2055283/range2055283/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274370/annotation2055283/range2055283/1
type: Range
persistent identity: range2055283
display id: range2055283
version: 1
direction: direction/forward
top level: BBa_K274370
start: 246
end: 325
orientation: inline

BBa_I746350
type: SequenceAnnotation
persistent identity: annotation2055282
display id: annotation2055282
version: 1
top level: BBa_K274370
location: https://synbiohub.org/public/igem/BBa_K274370/annotation2055282/range2055282/1
component: BBa_I746350

https://synbiohub.org/public/igem/BBa_K274370/annotation2055282/range2055282/1
type: Range
persistent identity: range2055282
display id: range2055282
version: 1
direction: direction/reverse
top level: BBa_K274370
start: 1
end: 237
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2055290
display id: annotation2055290
version: 1
top level: BBa_K274370
location: https://synbiohub.org/public/igem/BBa_K274370/annotation2055290/range2055290/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K274370/annotation2055290/range2055290/1
type: Range
persistent identity: range2055290
display id: range2055290
version: 1
direction: direction/reverse
top level: BBa_K274370
start: 383
end: 473
orientation: inline

BBa_P10003
type: ComponentDefinition
persistent identity: BBa P10003
display id: BBa_P10003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s cauliflower mosaic virus + omega tobacco mosaic virus
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:45:17Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP51266
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10003_sequence/1

BBa_M1004
type: ComponentDefinition
persistent identity: BBa M1004
display id: BBa_M1004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HlyD Membrane Protein
creator: Alex Hatch
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
mutable notes: Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
mutable provenance: Genomic. Escherichia coli UTI89
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HlyD
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1004_sequence/1

HlyD
type: SequenceAnnotation
persistent identity: annotation2115953
display id: annotation2115953
version: 1
top level: BBa_M1004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1

https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
type: Range
persistent identity: range2115953
display id: range2115953
version: 1
direction: direction/forward
top level: BBa_M1004
start: 1
end: 1434
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115952
display id: annotation2115952
version: 1
top level: BBa_M1004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1

https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
type: Range
persistent identity: range2115952
display id: range2115952
version: 1
direction: direction/forward
top level: BBa_M1004
start: 1
end: 3
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115954
display id: annotation2115954
version: 1
top level: BBa_M1004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1

https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
type: Range
persistent identity: range2115954
display id: range2115954
version: 1
direction: direction/forward
top level: BBa_M1004
start: 1435
end: 1437
orientation: inline

BBa_K909000
type: ComponentDefinition
persistent identity: BBa K909000
display id: BBa_K909000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phycocyanobilin synthesis operon
creator: Isaak Mueller
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14050
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_I15009
component: BBa_I15008
sequence annotation: BBa_I15008
sequence annotation: BBa_I15009
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K909000_sequence/1

BBa_B0034
type: Component
persistent identity: component2251128
display id: component2251128
version: 1
top level: BBa_K909000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15009
type: Component
persistent identity: component2251135
display id: component2251135
version: 1
top level: BBa_K909000
access: public
definition: https://synbiohub.org/public/igem/BBa_I15009/1

BBa_I15008
type: Component
persistent identity: component2251130
display id: component2251130
version: 1
top level: BBa_K909000
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_B0034
type: Component
persistent identity: component2251132
display id: component2251132
version: 1
top level: BBa_K909000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2251130
display id: annotation2251130
version: 1
top level: BBa_K909000
location: https://synbiohub.org/public/igem/BBa_K909000/annotation2251130/range2251130/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K909000/annotation2251130/range2251130/1
type: Range
persistent identity: range2251130
display id: range2251130
version: 1
direction: direction/reverse
top level: BBa_K909000
start: 19
end: 769
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2251135
display id: annotation2251135
version: 1
top level: BBa_K909000
location: https://synbiohub.org/public/igem/BBa_K909000/annotation2251135/range2251135/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K909000/annotation2251135/range2251135/1
type: Range
persistent identity: range2251135
display id: range2251135
version: 1
direction: direction/reverse
top level: BBa_K909000
start: 796
end: 1570
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2251128
display id: annotation2251128
version: 1
top level: BBa_K909000
location: https://synbiohub.org/public/igem/BBa_K909000/annotation2251128/range2251128/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909000/annotation2251128/range2251128/1
type: Range
persistent identity: range2251128
display id: range2251128
version: 1
direction: direction/reverse
top level: BBa_K909000
start: 1
end: 12
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2251132
display id: annotation2251132
version: 1
top level: BBa_K909000
location: https://synbiohub.org/public/igem/BBa_K909000/annotation2251132/range2251132/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K909000/annotation2251132/range2251132/1
type: Range
persistent identity: range2251132
display id: range2251132
version: 1
direction: direction/reverse
top level: BBa_K909000
start: 778
end: 789
orientation: inline

lamB
type: ComponentDefinition
persistent identity: BBa K137112
display id: BBa_K137112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lamB
creator: Fei Chen
created: 2008-09-23T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 2988
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lamB is the a porin protein which is part of the maltose pathway. It is also the receptor associated and required fro lambda phage infection.
mutable notes: N/A
mutable provenance: DH10B Genomic DNA Prep
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K137112_sequence/1

BBa_K173007
type: ComponentDefinition
persistent identity: BBa K173007
display id: BBa_K173007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aTc inducible system (aTc->PoPS) with J23100 promoter
creator: Lorenzo Pasotti, Letizia Diamante, Paolo Magni
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _281_
m user id: 0
owner id: 2583
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A
mutable notes: a
mutable provenance: A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K173005
component: BBa_J23100
sequence annotation: BBa_K173005
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K173007_sequence/1

BBa_K173005
type: Component
persistent identity: component2263005
display id: component2263005
version: 1
top level: BBa_K173007
access: public
definition: https://synbiohub.org/public/igem/BBa_K173005/1

BBa_J23100
type: Component
persistent identity: component2262986
display id: component2262986
version: 1
top level: BBa_K173007
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K173005
type: SequenceAnnotation
persistent identity: annotation2263005
display id: annotation2263005
version: 1
top level: BBa_K173007
location: https://synbiohub.org/public/igem/BBa_K173007/annotation2263005/range2263005/1
component: BBa_K173005

https://synbiohub.org/public/igem/BBa_K173007/annotation2263005/range2263005/1
type: Range
persistent identity: range2263005
display id: range2263005
version: 1
direction: direction/reverse
top level: BBa_K173007
start: 44
end: 945
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2262986
display id: annotation2262986
version: 1
top level: BBa_K173007
location: https://synbiohub.org/public/igem/BBa_K173007/annotation2262986/range2262986/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K173007/annotation2262986/range2262986/1
type: Range
persistent identity: range2262986
display id: range2262986
version: 1
direction: direction/reverse
top level: BBa_K173007
start: 1
end: 35
orientation: inline

BBa_P10301
type: ComponentDefinition
persistent identity: BBa P10301
display id: BBa_P10301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NptII
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:55:59Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhtyoBricks part from plasmid pUAP80037
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10301_sequence/1

"Eye 2"
type: ComponentDefinition
persistent identity: BBa K1080011
display id: BBa_K1080011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ChlI2
creator: Macquarie University
created: 2013-09-21T11:00:00Z
modified: 2016-10-20T07:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 7708
owner id: 18463
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Long Description here
mutable notes: Design Considerations here
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ChlI2
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1080011_sequence/1

ChlI2
type: SequenceAnnotation
persistent identity: annotation2525682
display id: annotation2525682
version: 1
top level: "Eye 2"
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1080011/annotation2525682/range2525682/1

https://synbiohub.org/public/igem/BBa_K1080011/annotation2525682/range2525682/1
type: Range
persistent identity: range2525682
display id: range2525682
version: 1
direction: direction/forward
top level: "Eye 2"
start: 25
end: 1206
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2525681
display id: annotation2525681
version: 1
top level: "Eye 2"
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1080011/annotation2525681/range2525681/1

https://synbiohub.org/public/igem/BBa_K1080011/annotation2525681/range2525681/1
type: Range
persistent identity: range2525681
display id: range2525681
version: 1
direction: direction/forward
top level: "Eye 2"
start: 6
end: 16
orientation: inline

BBa_J61032
type: ComponentDefinition
persistent identity: BBa J61032
display id: BBa_J61032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alkaline phosphatase [phoA]
creator: John Anderson
created: 2006-11-10T12:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Alkaline phosphatase coding sequence (no rbs, promoter, or terminator present) from Citrobacter. This bacterium's phoA was chosen due simply to the absense of Biobrick restriction sites.
mutable notes: N/A
mutable provenance: pre-pcr ca1033F/ca1032R on Citrobacter gen (1500 bp) PCR ca1032F/R on above PCR (1500 bp, XbaI/EcoRI) Sub into pSB1A2-Bca1008 (XbaI/MfeI, 2077+935, large) Product is pSB1A2-Bca1032 ---- ca1032F Forward oligo to biobrick phoA of Citrobacter GGTTAtctagaTGTTTTTTATTTTAACTGTATTTATACATGGAG ca1032R Reverse oligo to biobrick phoA of Citrobacter cagtaGAATTCccagaggcggcaTTACTTCAG ca1033F Forward constant oligo to clone phoA ttatcatcgctccgcatcctg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J61032_sequence/1

BBa_K180000
type: ComponentDefinition
persistent identity: BBa K180000
display id: BBa_K180000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter (trp & lac regulated -- tac pR)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. The Ptac sequence has been extracted by PCR from the commercially available plasmid pMAL-pIII from New England Biolabs (N8101S), with the addition of the biobrick prefix and suffix sequences. [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
mutable notes: Sequence includes lacIq and Shine-Dalgarno sequence from pMAL-pIII prior to the Ptac promoter to ensure correct functionality of the promoter.
mutable provenance: pMAL-pIII (New England Biolabs N8101S)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Ptac
sequence annotation: lacIq
sequence: https://synbiohub.org/public/igem/BBa_K180000_sequence/1

Ptac
type: SequenceAnnotation
persistent identity: annotation2044121
display id: annotation2044121
version: 1
top level: BBa_K180000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180000/annotation2044121/range2044121/1

https://synbiohub.org/public/igem/BBa_K180000/annotation2044121/range2044121/1
type: Range
persistent identity: range2044121
display id: range2044121
version: 1
direction: direction/forward
top level: BBa_K180000
start: 1325
end: 1352
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2044120
display id: annotation2044120
version: 1
top level: BBa_K180000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180000/annotation2044120/range2044120/1

https://synbiohub.org/public/igem/BBa_K180000/annotation2044120/range2044120/1
type: Range
persistent identity: range2044120
display id: range2044120
version: 1
direction: direction/forward
top level: BBa_K180000
start: 1
end: 1082
orientation: inline

BBa_K1051257
type: ComponentDefinition
persistent identity: BBa K1051257
display id: BBa_K1051257
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Construct the measurement pathway of K1051206.
creator: Rui Guan
created: 2013-09-01T11:00:00Z
modified: 2015-05-08T01:08:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 14515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Construct the final pathway with M0050
mutable notes: Construct the final pathway with M0050
mutable provenance: Construct the final pathway with M0050
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1051206
component: BBa_K1051000
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K1051206
sequence annotation: BBa_K1051000
sequence: https://synbiohub.org/public/igem/BBa_K1051257_sequence/1

BBa_K1051206
type: Component
persistent identity: component2362656
display id: component2362656
version: 1
top level: BBa_K1051257
access: public
definition: https://synbiohub.org/public/igem/BBa_K1051206/1

BBa_K1051000
type: Component
persistent identity: component2362655
display id: component2362655
version: 1
top level: BBa_K1051257
access: public
definition: BBa_K1051000

BBa_J04500
type: Component
persistent identity: component2362654
display id: component2362654
version: 1
top level: BBa_K1051257
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2362654
display id: annotation2362654
version: 1
top level: BBa_K1051257
location: https://synbiohub.org/public/igem/BBa_K1051257/annotation2362654/range2362654/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1051257/annotation2362654/range2362654/1
type: Range
persistent identity: range2362654
display id: range2362654
version: 1
direction: direction/reverse
top level: BBa_K1051257
start: 1
end: 220
orientation: inline

BBa_K1051206
type: SequenceAnnotation
persistent identity: annotation2362656
display id: annotation2362656
version: 1
top level: BBa_K1051257
location: https://synbiohub.org/public/igem/BBa_K1051257/annotation2362656/range2362656/1
component: BBa_K1051206

https://synbiohub.org/public/igem/BBa_K1051257/annotation2362656/range2362656/1
type: Range
persistent identity: range2362656
display id: range2362656
version: 1
direction: direction/reverse
top level: BBa_K1051257
start: 910
end: 948
orientation: inline

BBa_K1051000
type: SequenceAnnotation
persistent identity: annotation2362655
display id: annotation2362655
version: 1
top level: BBa_K1051257
location: https://synbiohub.org/public/igem/BBa_K1051257/annotation2362655/range2362655/1
component: BBa_K1051000

https://synbiohub.org/public/igem/BBa_K1051257/annotation2362655/range2362655/1
type: Range
persistent identity: range2362655
display id: range2362655
version: 1
direction: direction/reverse
top level: BBa_K1051257
start: 227
end: 901
orientation: inline

BBa_K112305
type: ComponentDefinition
persistent identity: BBa K112305
display id: BBa_K112305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.lambda lysozyme!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda lysozyme with native rbs site in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0006/al0002 on pSB3C6-Bjh1501 (527bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112305 ----------------------------------------------- al0006 Forward Biobricking of lambda lysozyme with rbs site CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg al0002 Reverse Biobricking of lambda lysozyme with rbs site CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112305_sequence/1

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E1010
component: BBa_R0010
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_K343000
type: ComponentDefinition
persistent identity: BBa K343000
display id: BBa_K343000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated flagella master regulator (FlhDC mut).
creator: Lars Christian Lund
created: 2010-07-01T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6081
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The master regulator operon for flagellum synthesis. Produces transcriptional factors for Class II flagellar regulon genes.
mutable notes: The sequence comes directly from E.Coli.
mutable provenance: E.Coli K12 Mg1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Changed a T to C to remove Pst1 restriction site.
sequence annotation: Start codon
sequence annotation: Stop codon
sequence annotation: FlhD
sequence annotation: FlhC
sequence: https://synbiohub.org/public/igem/BBa_K343000_sequence/1

Changed a T to C to remove Pst1 restriction site.
type: SequenceAnnotation
persistent identity: annotation2072413
display id: annotation2072413
version: 1
top level: BBa_K343000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K343000/annotation2072413/range2072413/1

https://synbiohub.org/public/igem/BBa_K343000/annotation2072413/range2072413/1
type: Range
persistent identity: range2072413
display id: range2072413
version: 1
direction: direction/forward
top level: BBa_K343000
start: 822
end: 822
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2072345
display id: annotation2072345
version: 1
top level: BBa_K343000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K343000/annotation2072345/range2072345/1

https://synbiohub.org/public/igem/BBa_K343000/annotation2072345/range2072345/1
type: Range
persistent identity: range2072345
display id: range2072345
version: 1
direction: direction/forward
top level: BBa_K343000
start: 356
end: 358
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2072347
display id: annotation2072347
version: 1
top level: BBa_K343000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K343000/annotation2072347/range2072347/1

https://synbiohub.org/public/igem/BBa_K343000/annotation2072347/range2072347/1
type: Range
persistent identity: range2072347
display id: range2072347
version: 1
direction: direction/forward
top level: BBa_K343000
start: 930
end: 932
orientation: inline

FlhD
type: SequenceAnnotation
persistent identity: annotation2072343
display id: annotation2072343
version: 1
top level: BBa_K343000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K343000/annotation2072343/range2072343/1

https://synbiohub.org/public/igem/BBa_K343000/annotation2072343/range2072343/1
type: Range
persistent identity: range2072343
display id: range2072343
version: 1
direction: direction/forward
top level: BBa_K343000
start: 1
end: 349
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2072344
display id: annotation2072344
version: 1
top level: BBa_K343000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K343000/annotation2072344/range2072344/1

https://synbiohub.org/public/igem/BBa_K343000/annotation2072344/range2072344/1
type: Range
persistent identity: range2072344
display id: range2072344
version: 1
direction: direction/forward
top level: BBa_K343000
start: 350
end: 352
orientation: inline

FlhC
type: SequenceAnnotation
persistent identity: annotation2072346
display id: annotation2072346
version: 1
top level: BBa_K343000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K343000/annotation2072346/range2072346/1

https://synbiohub.org/public/igem/BBa_K343000/annotation2072346/range2072346/1
type: Range
persistent identity: range2072346
display id: range2072346
version: 1
direction: direction/forward
top level: BBa_K343000
start: 359
end: 929
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2072342
display id: annotation2072342
version: 1
top level: BBa_K343000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K343000/annotation2072342/range2072342/1

https://synbiohub.org/public/igem/BBa_K343000/annotation2072342/range2072342/1
type: Range
persistent identity: range2072342
display id: range2072342
version: 1
direction: direction/forward
top level: BBa_K343000
start: 1
end: 3
orientation: inline

BBa_K215001
type: ComponentDefinition
persistent identity: BBa K215001
display id: BBa_K215001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretion Signal and Streptavidin Binding Tags
creator: Jeff Nivala
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is the vector into which afp is inserted to tag it for purification using the IPP system. It contains an NheI restriction site that allows for the insertion of the desired protein coding sequence (NheI is compatible with XbaI and SpeI). This Target Vector part contains two tags: a secretion tag that causes the protein to be exported to the extracellular space, and a nanotag that causes the protein to bind to stretavidin (or some analog). For this part to function properly, it must be combined with a promoter and RBS, and used in E.coli strain UW5alpha, which has two other plasmids containing the other genes required for proper secretion and display of the protein to be purified on the outer membrane.
mutable notes: NheI was chosen as the protein insertion site because it is compatible with digested XbaI and SpeI restriction sites. The TEV sites and HIS tags flanking the NheI site are present to separate the inserted protein from the added tags. GSA linkers were used to separate the various features of the part.
mutable provenance: Nanotag (bases 1-48): From Lamla and Erdmann (http://www.ncbi.nlm.nih.gov/pubmed/14680960). Secretion tag (last 181 AA): Gene Bank ID Erwinia chrysanthemi M60395.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: TEV protease site
sequence annotation: His tag
sequence annotation: Secretion tag
sequence annotation: nanotag
sequence annotation: GSA linker
sequence annotation: terminator (B0015)
sequence annotation: NheI
sequence: https://synbiohub.org/public/igem/BBa_K215001_sequence/1

TEV protease site
type: SequenceAnnotation
persistent identity: annotation2027595
display id: annotation2027595
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027595/range2027595/1

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type: Range
persistent identity: range2027595
display id: range2027595
version: 1
direction: direction/forward
top level: BBa_K215001
start: 130
end: 150
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2027589
display id: annotation2027589
version: 1
top level: BBa_K215001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027589/range2027589/1

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type: Range
persistent identity: range2027589
display id: range2027589
version: 1
direction: direction/forward
top level: BBa_K215001
start: 58
end: 75
orientation: inline

Secretion tag
type: SequenceAnnotation
persistent identity: annotation2027599
display id: annotation2027599
version: 1
top level: BBa_K215001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027599/range2027599/1

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type: Range
persistent identity: range2027599
display id: range2027599
version: 1
direction: direction/forward
top level: BBa_K215001
start: 187
end: 735
orientation: inline

nanotag
type: SequenceAnnotation
persistent identity: annotation2027587
display id: annotation2027587
version: 1
top level: BBa_K215001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027587/range2027587/1

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type: Range
persistent identity: range2027587
display id: range2027587
version: 1
direction: direction/forward
top level: BBa_K215001
start: 1
end: 48
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027592
display id: annotation2027592
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027592/range2027592/1

https://synbiohub.org/public/igem/BBa_K215001/annotation2027592/range2027592/1
type: Range
persistent identity: range2027592
display id: range2027592
version: 1
direction: direction/forward
top level: BBa_K215001
start: 105
end: 114
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027594
display id: annotation2027594
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027594/range2027594/1

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type: Range
persistent identity: range2027594
display id: range2027594
version: 1
direction: direction/forward
top level: BBa_K215001
start: 121
end: 129
orientation: inline

terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2027600
display id: annotation2027600
version: 1
top level: BBa_K215001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027600/range2027600/1

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type: Range
persistent identity: range2027600
display id: range2027600
version: 1
direction: direction/forward
top level: BBa_K215001
start: 736
end: 865
orientation: inline

NheI
type: SequenceAnnotation
persistent identity: annotation2027593
display id: annotation2027593
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027593/range2027593/1

https://synbiohub.org/public/igem/BBa_K215001/annotation2027593/range2027593/1
type: Range
persistent identity: range2027593
display id: range2027593
version: 1
direction: direction/forward
top level: BBa_K215001
start: 115
end: 120
orientation: inline

TEV protease site
type: SequenceAnnotation
persistent identity: annotation2027591
display id: annotation2027591
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027591/range2027591/1

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type: Range
persistent identity: range2027591
display id: range2027591
version: 1
direction: direction/forward
top level: BBa_K215001
start: 85
end: 104
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027588
display id: annotation2027588
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027588/range2027588/1

https://synbiohub.org/public/igem/BBa_K215001/annotation2027588/range2027588/1
type: Range
persistent identity: range2027588
display id: range2027588
version: 1
direction: direction/forward
top level: BBa_K215001
start: 49
end: 57
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2027597
display id: annotation2027597
version: 1
top level: BBa_K215001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027597/range2027597/1

https://synbiohub.org/public/igem/BBa_K215001/annotation2027597/range2027597/1
type: Range
persistent identity: range2027597
display id: range2027597
version: 1
direction: direction/forward
top level: BBa_K215001
start: 160
end: 177
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027590
display id: annotation2027590
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027590/range2027590/1

https://synbiohub.org/public/igem/BBa_K215001/annotation2027590/range2027590/1
type: Range
persistent identity: range2027590
display id: range2027590
version: 1
direction: direction/forward
top level: BBa_K215001
start: 76
end: 84
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027596
display id: annotation2027596
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027596/range2027596/1

https://synbiohub.org/public/igem/BBa_K215001/annotation2027596/range2027596/1
type: Range
persistent identity: range2027596
display id: range2027596
version: 1
direction: direction/forward
top level: BBa_K215001
start: 151
end: 159
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027598
display id: annotation2027598
version: 1
top level: BBa_K215001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027598/range2027598/1

https://synbiohub.org/public/igem/BBa_K215001/annotation2027598/range2027598/1
type: Range
persistent identity: range2027598
display id: range2027598
version: 1
direction: direction/forward
top level: BBa_K215001
start: 178
end: 186
orientation: inline

BBa_K133010
type: ComponentDefinition
persistent identity: BBa K133010
display id: BBa_K133010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CF213
creator: Vid Kocar
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3351
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chimeric flagellin
mutable notes: None
mutable provenance: Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133010_sequence/1

BBa_K274380
type: ComponentDefinition
persistent identity: BBa K274380
display id: BBa_K274380
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> PSP3 pag - PF promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_I746360
component: BBa_B0010
component: BBa_I746351
sequence annotation: BBa_I746351
sequence annotation: BBa_B0010
sequence annotation: BBa_I746360
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K274380_sequence/1

BBa_B0012
type: Component
persistent identity: component2055333
display id: component2055333
version: 1
top level: BBa_K274380
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746360
type: Component
persistent identity: component2055338
display id: component2055338
version: 1
top level: BBa_K274380
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_B0010
type: Component
persistent identity: component2055331
display id: component2055331
version: 1
top level: BBa_K274380
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746351
type: Component
persistent identity: component2055330
display id: component2055330
version: 1
top level: BBa_K274380
access: public
definition: https://synbiohub.org/public/igem/BBa_I746351/1

BBa_I746351
type: SequenceAnnotation
persistent identity: annotation2055330
display id: annotation2055330
version: 1
top level: BBa_K274380
location: https://synbiohub.org/public/igem/BBa_K274380/annotation2055330/range2055330/1
component: BBa_I746351

https://synbiohub.org/public/igem/BBa_K274380/annotation2055330/range2055330/1
type: Range
persistent identity: range2055330
display id: range2055330
version: 1
direction: direction/reverse
top level: BBa_K274380
start: 1
end: 237
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055331
display id: annotation2055331
version: 1
top level: BBa_K274380
location: https://synbiohub.org/public/igem/BBa_K274380/annotation2055331/range2055331/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274380/annotation2055331/range2055331/1
type: Range
persistent identity: range2055331
display id: range2055331
version: 1
direction: direction/forward
top level: BBa_K274380
start: 246
end: 325
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2055338
display id: annotation2055338
version: 1
top level: BBa_K274380
location: https://synbiohub.org/public/igem/BBa_K274380/annotation2055338/range2055338/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K274380/annotation2055338/range2055338/1
type: Range
persistent identity: range2055338
display id: range2055338
version: 1
direction: direction/reverse
top level: BBa_K274380
start: 383
end: 473
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055333
display id: annotation2055333
version: 1
top level: BBa_K274380
location: https://synbiohub.org/public/igem/BBa_K274380/annotation2055333/range2055333/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274380/annotation2055333/range2055333/1
type: Range
persistent identity: range2055333
display id: range2055333
version: 1
direction: direction/forward
top level: BBa_K274380
start: 334
end: 374
orientation: inline

BBa_K208038
type: ComponentDefinition
persistent identity: BBa K208038
display id: BBa_K208038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Promoter/RBS/GeneIII/PhaP/Terminator
creator: USU iGEM 2009
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: PhaP Secretion
mutable notes: Silver Fusion
mutable provenance: Composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_K208001
component: BBa_B0010
component: BBa_B0034
component: BBa_K208002
component: BBa_B0012
sequence annotation: BBa_K208001
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K208002
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K208038_sequence/1

BBa_R0010
type: Component
persistent identity: component2056071
display id: component2056071
version: 1
top level: BBa_K208038
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K208001
type: Component
persistent identity: component2056082
display id: component2056082
version: 1
top level: BBa_K208038
access: public
definition: BBa_K208001

BBa_B0010
type: Component
persistent identity: component2056083
display id: component2056083
version: 1
top level: BBa_K208038
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2056079
display id: component2056079
version: 1
top level: BBa_K208038
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K208002
type: Component
persistent identity: component2056081
display id: component2056081
version: 1
top level: BBa_K208038
access: public
definition: https://synbiohub.org/public/igem/BBa_K208002/1

BBa_B0012
type: Component
persistent identity: component2056085
display id: component2056085
version: 1
top level: BBa_K208038
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K208001
type: SequenceAnnotation
persistent identity: annotation2056082
display id: annotation2056082
version: 1
top level: BBa_K208038
location: https://synbiohub.org/public/igem/BBa_K208038/annotation2056082/range2056082/1
component: BBa_K208001

https://synbiohub.org/public/igem/BBa_K208038/annotation2056082/range2056082/1
type: Range
persistent identity: range2056082
display id: range2056082
version: 1
direction: direction/reverse
top level: BBa_K208038
start: 287
end: 865
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056079
display id: annotation2056079
version: 1
top level: BBa_K208038
location: https://synbiohub.org/public/igem/BBa_K208038/annotation2056079/range2056079/1
component: BBa_B0034

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type: Range
persistent identity: range2056079
display id: range2056079
version: 1
direction: direction/reverse
top level: BBa_K208038
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056083
display id: annotation2056083
version: 1
top level: BBa_K208038
location: https://synbiohub.org/public/igem/BBa_K208038/annotation2056083/range2056083/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K208038/annotation2056083/range2056083/1
type: Range
persistent identity: range2056083
display id: range2056083
version: 1
direction: direction/forward
top level: BBa_K208038
start: 874
end: 953
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2056071
display id: annotation2056071
version: 1
top level: BBa_K208038
location: https://synbiohub.org/public/igem/BBa_K208038/annotation2056071/range2056071/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K208038/annotation2056071/range2056071/1
type: Range
persistent identity: range2056071
display id: range2056071
version: 1
direction: direction/forward
top level: BBa_K208038
start: 1
end: 200
orientation: inline

BBa_K208002
type: SequenceAnnotation
persistent identity: annotation2056081
display id: annotation2056081
version: 1
top level: BBa_K208038
location: https://synbiohub.org/public/igem/BBa_K208038/annotation2056081/range2056081/1
component: BBa_K208002

https://synbiohub.org/public/igem/BBa_K208038/annotation2056081/range2056081/1
type: Range
persistent identity: range2056081
display id: range2056081
version: 1
direction: direction/reverse
top level: BBa_K208038
start: 227
end: 280
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056085
display id: annotation2056085
version: 1
top level: BBa_K208038
location: https://synbiohub.org/public/igem/BBa_K208038/annotation2056085/range2056085/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K208038/annotation2056085/range2056085/1
type: Range
persistent identity: range2056085
display id: range2056085
version: 1
direction: direction/forward
top level: BBa_K208038
start: 962
end: 1002
orientation: inline

BisdAB
type: ComponentDefinition
persistent identity: BBa K525517
display id: BBa_K525517
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of BisdA and BisdB behind constitutive promoter
creator: Jonas Aretz
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion protein of BisdA and BisdB behind constitutive promoter
mutable notes: Fusion protein of BisdA and BisdB behind constitutive promoter
mutable provenance: Assembled
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J23110
component: BBa_B0034
component: BBa_K525515
sequence annotation: BBa_K525515
sequence annotation: BBa_B0034
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K525517_sequence/1

BBa_J23110
type: Component
persistent identity: component2128146
display id: component2128146
version: 1
top level: BisdAB
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0034
type: Component
persistent identity: component2128148
display id: component2128148
version: 1
top level: BisdAB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K525515
type: Component
persistent identity: component2128151
display id: component2128151
version: 1
top level: BisdAB
access: public
definition: https://synbiohub.org/public/igem/BBa_K525515/1

BBa_K525515
type: SequenceAnnotation
persistent identity: annotation2128151
display id: annotation2128151
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128151/range2128151/1
component: BBa_K525515

https://synbiohub.org/public/igem/BBa_K525517/annotation2128151/range2128151/1
type: Range
persistent identity: range2128151
display id: range2128151
version: 1
direction: direction/reverse
top level: BisdAB
start: 62
end: 1663
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128148
display id: annotation2128148
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128148/range2128148/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K525517/annotation2128148/range2128148/1
type: Range
persistent identity: range2128148
display id: range2128148
version: 1
direction: direction/reverse
top level: BisdAB
start: 44
end: 55
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2128146
display id: annotation2128146
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128146/range2128146/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K525517/annotation2128146/range2128146/1
type: Range
persistent identity: range2128146
display id: range2128146
version: 1
direction: direction/reverse
top level: BisdAB
start: 1
end: 35
orientation: inline

d6des
type: ComponentDefinition
persistent identity: BBa K925003
display id: BBa_K925003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Delta-6 desaturase
creator: Veronica Rezelj
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1190_
m user id: 0
owner id: 12788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part encodes a Δ6 desaturase derived from Synechocystis sp PCC 6803. The enzyme is a membrane-bound protein able to introduce a double bond at the Δ-12 site in the hydrocarbon chain of oleic acid (18:1, Δ9). This converts the substrate into linoleic acid (18:2 Δ9,12), a polyunsaturated fatty acid (PUFA). Mass spectrometry results show that feeding oleic acid to transformed E. coli, the bacterial membrane composition changes. Linoleic acid is observed.
mutable notes: -
mutable provenance: This desaturase comes from Synechocystis sp PCC 6803 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: d12des
sequence: https://synbiohub.org/public/igem/BBa_K925003_sequence/1

d12des
type: SequenceAnnotation
persistent identity: annotation2196235
display id: annotation2196235
version: 1
top level: d6des
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K925003/annotation2196235/range2196235/1

https://synbiohub.org/public/igem/BBa_K925003/annotation2196235/range2196235/1
type: Range
persistent identity: range2196235
display id: range2196235
version: 1
direction: direction/forward
top level: d6des
start: 1
end: 1080
orientation: inline

BBa_I763026
type: ComponentDefinition
persistent identity: BBa I763026
display id: BBa_I763026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI coding device regulated by pTetR
creator: Alice Pasini
created: 2007-09-05T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI coding device regulated by pTet inverting regulator which action is inhibited by the addition of tetracycline or its analog, aTc.
mutable notes: ---
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I763025
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_I763025
sequence: https://synbiohub.org/public/igem/BBa_I763026_sequence/1

BBa_I763025
type: Component
persistent identity: component2253079
display id: component2253079
version: 1
top level: BBa_I763026
access: public
definition: https://synbiohub.org/public/igem/BBa_I763025/1

BBa_B0015
type: Component
persistent identity: component2253086
display id: component2253086
version: 1
top level: BBa_I763026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2253086
display id: annotation2253086
version: 1
top level: BBa_I763026
location: https://synbiohub.org/public/igem/BBa_I763026/annotation2253086/range2253086/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I763026/annotation2253086/range2253086/1
type: Range
persistent identity: range2253086
display id: range2253086
version: 1
direction: direction/reverse
top level: BBa_I763026
start: 1242
end: 1370
orientation: inline

BBa_I763025
type: SequenceAnnotation
persistent identity: annotation2253079
display id: annotation2253079
version: 1
top level: BBa_I763026
location: https://synbiohub.org/public/igem/BBa_I763026/annotation2253079/range2253079/1
component: BBa_I763025

https://synbiohub.org/public/igem/BBa_I763026/annotation2253079/range2253079/1
type: Range
persistent identity: range2253079
display id: range2253079
version: 1
direction: direction/reverse
top level: BBa_I763026
start: 1
end: 1233
orientation: inline

Hin
type: ComponentDefinition
persistent identity: BBa J31000
display id: BBa_J31000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA-invertase Hin from Salmonella typhimurium
creator: Erin Zwack, Sabriya Rosemond
created: 2006-06-04T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix siteds.
mutable notes: none really
mutable provenance: Salmonella typhimurium
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hin sequence
sequence annotation: Mutation to delete the SpeI site
sequence annotation: Fwd primer J31001
sequence annotation: Former SpeI site
sequence annotation: Rev primer J31001
sequence: https://synbiohub.org/public/igem/BBa_J31000_sequence/1

Hin sequence
type: SequenceAnnotation
persistent identity: annotation1884987
display id: annotation1884987
version: 1
top level: Hin
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
type: Range
persistent identity: range1884987
display id: range1884987
version: 1
direction: direction/forward
top level: Hin
start: 1
end: 573
orientation: inline

Mutation to delete the SpeI site
type: SequenceAnnotation
persistent identity: annotation1880460
display id: annotation1880460
version: 1
top level: Hin
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
type: Range
persistent identity: range1880460
display id: range1880460
version: 1
direction: direction/forward
top level: Hin
start: 72
end: 72
orientation: inline

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910568
display id: annotation1910568
version: 1
top level: Hin
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
type: Range
persistent identity: range1910568
display id: range1910568
version: 1
direction: direction/forward
top level: Hin
start: 1
end: 3
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880459
display id: annotation1880459
version: 1
top level: Hin
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
type: Range
persistent identity: range1880459
display id: range1880459
version: 1
direction: direction/forward
top level: Hin
start: 70
end: 75
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910569
display id: annotation1910569
version: 1
top level: Hin
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
type: Range
persistent identity: range1910569
display id: range1910569
version: 1
direction: direction/reverse
top level: Hin
start: 551
end: 570
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa K1088005
display id: BBa_K1088005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: arabinose regulator (araC) coding sequence
creator: Andreas Kj??r
created: 2013-08-19T11:00:00Z
modified: 2015-05-08T01:09:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This sequence is a copy of the sequence 70,387 -> 71,265 in E. coli K-12 MG1655 which is the coding sequence of the pBAD repressor araC. From ECOCYC: The "arabinose regulator," AraC, is a transcription factor that regulates transcription of several genes and operons involved in arabinose catabolism and transport. It coregulates with another transcriptional regulator, CRP; both are transcription factors involved in l-arabinose degradation. These regulators bind cooperatively to activate transcription of five operons related to transport, catabolism, and autoregulation of l-arabinose. Transcription of these operons is induced when E. coli is grown in the absence of glucose and when the physiological inducer, l-arabinose, binds to the AraC regulator. In the absence of glucose, cellular cyclic AMP levels are high and cyclic AMP forms a dimeric complex with CRP to coregulate with AraC. For more info go to http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10054
mutable notes: The part was amplified from chromosomal DNA with primers carrying prefix and suffix overhangs
mutable provenance: E. coli K-12 MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: araC coding region
sequence: https://synbiohub.org/public/igem/BBa_K1088005_sequence/1

araC coding region
type: SequenceAnnotation
persistent identity: annotation2331909
display id: annotation2331909
version: 1
top level: araC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1088005/annotation2331909/range2331909/1

https://synbiohub.org/public/igem/BBa_K1088005/annotation2331909/range2331909/1
type: Range
persistent identity: range2331909
display id: range2331909
version: 1
direction: direction/forward
top level: araC
start: 1
end: 879
orientation: inline

gfasPurple
type: ComponentDefinition
persistent identity: BBa K1033919
display id: BBa_K1033919
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This chromoprotein from the coral Galaxea fascicularis, gfasPurple, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The coral Galaxea fascicularis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gfasPurple
sequence: https://synbiohub.org/public/igem/BBa_K1033919_sequence/1

gfasPurple
type: SequenceAnnotation
persistent identity: annotation2332104
display id: annotation2332104
version: 1
top level: gfasPurple
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033919/annotation2332104/range2332104/1

https://synbiohub.org/public/igem/BBa_K1033919/annotation2332104/range2332104/1
type: Range
persistent identity: range2332104
display id: range2332104
version: 1
direction: direction/forward
top level: gfasPurple
start: 1
end: 666
orientation: inline

BBa_K1149035
type: ComponentDefinition
persistent identity: BBa K1149035
display id: BBa_K1149035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Proteinase K
creator: Margarita Kopniczky
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:26Z
discontinued: false
dominant: false
group u list: _1461_
m user id: 0
owner id: 17968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Encodes a peptidase from proteinase K family, breaks down polylactic acid
mutable notes: E.coli optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6his+stop
sequence: https://synbiohub.org/public/igem/BBa_K1149035_sequence/1

6his+stop
type: SequenceAnnotation
persistent identity: annotation2340864
display id: annotation2340864
version: 1
top level: BBa_K1149035
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1149035/annotation2340864/range2340864/1

https://synbiohub.org/public/igem/BBa_K1149035/annotation2340864/range2340864/1
type: Range
persistent identity: range2340864
display id: range2340864
version: 1
direction: direction/forward
top level: BBa_K1149035
start: 1116
end: 1137
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa K165004
display id: BBa_K165004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, yeast optimized for fusion
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the mCherry fluorescent protein, yeast-codon optimized and biobricked in the silver lab BioFusion standard for use in fusion proteins.
mutable notes: -
mutable provenance: From Caroline Ajo-Franklin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165004_sequence/1

BBa_K404112
type: ComponentDefinition
persistent identity: BBa K404112
display id: BBa_K404112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cytosine deaminase (CD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Cytosine deaminase
sequence: https://synbiohub.org/public/igem/BBa_K404112_sequence/1

Cytosine deaminase
type: SequenceAnnotation
persistent identity: annotation2093175
display id: annotation2093175
version: 1
top level: BBa_K404112
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404112/annotation2093175/range2093175/1

https://synbiohub.org/public/igem/BBa_K404112/annotation2093175/range2093175/1
type: Range
persistent identity: range2093175
display id: range2093175
version: 1
direction: direction/forward
top level: BBa_K404112
start: 1
end: 1281
orientation: inline

BBa_K228005
type: ComponentDefinition
persistent identity: BBa K228005
display id: BBa_K228005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC protein and Pbad promoter
creator: Gao Rencheng
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It generates AraC regulatory protein which binds to and repress the pBad promoter(such as BBa_I13453). L-arabinose, which acts as inducer, could efficiently prevent the protein binding to pBad.
mutable provenance: Partsregistry, MIT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K228007
component: BBa_I13453
sequence annotation: BBa_K228007
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228005_sequence/1

BBa_K228007
type: Component
persistent identity: component2262289
display id: component2262289
version: 1
top level: BBa_K228005
access: public
definition: https://synbiohub.org/public/igem/BBa_K228007/1

BBa_I13453
type: Component
persistent identity: component2262290
display id: component2262290
version: 1
top level: BBa_K228005
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K228007
type: SequenceAnnotation
persistent identity: annotation2262289
display id: annotation2262289
version: 1
top level: BBa_K228005
location: https://synbiohub.org/public/igem/BBa_K228005/annotation2262289/range2262289/1
component: BBa_K228007

https://synbiohub.org/public/igem/BBa_K228005/annotation2262289/range2262289/1
type: Range
persistent identity: range2262289
display id: range2262289
version: 1
direction: direction/reverse
top level: BBa_K228005
start: 1
end: 1141
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2262290
display id: annotation2262290
version: 1
top level: BBa_K228005
location: https://synbiohub.org/public/igem/BBa_K228005/annotation2262290/range2262290/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228005/annotation2262290/range2262290/1
type: Range
persistent identity: range2262290
display id: range2262290
version: 1
direction: direction/reverse
top level: BBa_K228005
start: 1150
end: 1279
orientation: inline

BBa_P10004
type: ComponentDefinition
persistent identity: BBa P10004
display id: BBa_P10004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mas
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:46:44Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP85281
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10004_sequence/1

TEV
type: ComponentDefinition
persistent identity: BBa K316012
display id: BBa_K316012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEV protease S219P autocatalysis resistant variant
creator: IC 2010 Team
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: TEV protease S219P autocatalysis resistant variant Introduction : This is the nuclear inclusion protease, endogenous to Tobacco Etch Virus and is used in the late lifecycle to cleave polyprotein precursors. The recognition sequence is ENLYFQG/S [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W9V-45PMGK3-9P&_user=217827&_coverDate=02%2F01%2F1994&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=e075aad3a349720ad9484095d01a65be&searchtype=a]] between QG or QSDue to it???s stringent sequence specificity, TEV is commonly used to cleave genetically engineered proteins. Uses: TEV proteinase is used to cleave fusion proteins. It is useful due to its high degree of specificity http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W9V-45PMGK3-9P&_user=217827&_coverDate=02%2F01%2F1994&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=e075aad3a349720ad9484095d01a65be&searchtype=a and potential to be used in vivo or in vitro applications. Auto-inactivation Wild type TEV protease also cleaves itself at Met 218 and Ser 219 http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WXR-45R86R6-5X&_user=7635175&_coverDate=06%2F20%2F1995&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000011279&_version=1&_urlVersion=0&_userid=7635175&md5=0a1521e783c0bd4126b145f3f6d766d4&searchtype=a . This leads to auto-inactivation of the TEV protease and progressive loss of activity of the protein. The rate of inactivation is proportional to the concentration of protease http://peds.oxfordjournals.org/content/14/12/993.long More stable Mutants have been produced by single amino acid substitutions S219V (AGC(serine) to GTG(valine) and S219P (AGC(serine) to CCG(proline) Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type…
mutable notes: The part was produced by nucleotide synthesis by mwg ??? eurofin
mutable provenance: TEV protease is naturally found in Tobacco Etch Virus genome. This part contains the sequence codon optimized for expression in B.subtilis using mwg ??? eurofins www.eurofinsdna.com proprietary software.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TEV protease
sequence: https://synbiohub.org/public/igem/BBa_K316012_sequence/1

TEV protease
type: SequenceAnnotation
persistent identity: annotation2100977
display id: annotation2100977
version: 1
top level: TEV
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K316012/annotation2100977/range2100977/1

https://synbiohub.org/public/igem/BBa_K316012/annotation2100977/range2100977/1
type: Range
persistent identity: range2100977
display id: range2100977
version: 1
direction: direction/reverse
top level: TEV
start: 1
end: 717
orientation: inline

BBa_K404111
type: ComponentDefinition
persistent identity: BBa K404111
display id: BBa_K404111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mouse guanylate kinase (mGMK)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: mouse guanylate kinase
sequence: https://synbiohub.org/public/igem/BBa_K404111_sequence/1

mouse guanylate kinase
type: SequenceAnnotation
persistent identity: annotation2093174
display id: annotation2093174
version: 1
top level: BBa_K404111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404111/annotation2093174/range2093174/1

https://synbiohub.org/public/igem/BBa_K404111/annotation2093174/range2093174/1
type: Range
persistent identity: range2093174
display id: range2093174
version: 1
direction: direction/forward
top level: BBa_K404111
start: 1
end: 585
orientation: inline

BBa_K249009
type: ComponentDefinition
persistent identity: BBa K249009
display id: BBa_K249009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP with N-terminal Arganine Fusion Tag
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: true
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This Cyan Fluorescent protein has been fused with 10 N-terminal Arginines to target it into the Lumazine-based microcompartment. Has a double terminator
mutable notes: Standard
mutable provenance: BioBrick CFP with synthesized N-terminal Arg Tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K249009_sequence/1

BBa_P10598
type: ComponentDefinition
persistent identity: BBa P10598
display id: BBa_P10598
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenBraid destination vector omega insert
creator: Vinoo Selvarajah
created: 2016-06-16T11:00:00Z
modified: 2016-06-17T04:44:37Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 4206
owner id: 4206
owning group id: 1
status: status/Planning
bookmark: false
mutable description: More info to come
mutable notes: More info to come
mutable provenance: More info to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10598_sequence/1

lacI ts
type: ComponentDefinition
persistent identity: BBa J06501
display id: BBa_J06501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressor (temperature-sensitive mut 265) (+LVA)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature sensitive. Mutation from Chao, et al., 2002: Gly265->Asp (GG(803)T->GAT)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: TS mutation (G->A)
sequence annotation: barcode from LacI (BBa_C0012)
sequence annotation: LacI(ts)-LVA
sequence: https://synbiohub.org/public/igem/BBa_J06501_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1530907
display id: annotation1530907
version: 1
top level: lacI ts
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530907/range1530907/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530907/range1530907/1
type: Range
persistent identity: range1530907
display id: range1530907
version: 1
direction: direction/forward
top level: lacI ts
start: 1090
end: 1128
orientation: inline

TS mutation (G->A)
type: SequenceAnnotation
persistent identity: annotation1530900
display id: annotation1530900
version: 1
top level: lacI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530900/range1530900/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530900/range1530900/1
type: Range
persistent identity: range1530900
display id: range1530900
version: 1
direction: direction/forward
top level: lacI ts
start: 803
end: 803
orientation: inline

barcode from LacI (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1530914
display id: annotation1530914
version: 1
top level: lacI ts
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530914/range1530914/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530914/range1530914/1
type: Range
persistent identity: range1530914
display id: range1530914
version: 1
direction: direction/forward
top level: lacI ts
start: 1129
end: 1153
orientation: inline

LacI(ts)-LVA
type: SequenceAnnotation
persistent identity: annotation1530899
display id: annotation1530899
version: 1
top level: lacI ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530899/range1530899/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530899/range1530899/1
type: Range
persistent identity: range1530899
display id: range1530899
version: 1
direction: direction/forward
top level: lacI ts
start: 1
end: 1128
orientation: inline

pET fusion
type: ComponentDefinition
persistent identity: BBa K1122673
display id: BBa_K1122673
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ethanol production module
creator: Aleksandra Lewicka, Jan Lyczakowski
created: 2013-08-15T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1433_
m user id: 0
owner id: 18014
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part codes for the fusion of Pyruvate decarboxylase and Alcohol dehydrogenase from ''Zymomonas mobilis''. Fused enzymes increase ethanol yields and enable its faster production.
mutable notes: The part is based on the BBa_K173003 - the enzymatic components of this BioBrick were fused using MABEL technology. The fusion removed start codon in adhB, stop codon in pdc and the RBS inbetween.
mutable provenance: ''Z. mobilis'' gDNA codon optimised for ''E. coli''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122673_sequence/1

BBa_P10002
type: ComponentDefinition
persistent identity: BBa P10002
display id: BBa_P10002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pro+5U_AtUbi10
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:44:01Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP12015
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10002_sequence/1

BBa_K496003
type: ComponentDefinition
persistent identity: BBa K496003
display id: BBa_K496003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SlrP secretion tag, salmonella T3 sec. sys.
creator: Shunichi MAKINO
created: 2010-10-16T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _575_
m user id: 0
owner id: 6855
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: leucine-rich repeat protein (SlrP) One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional restrictions of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins like GFP. Currently this part is in trials to see if it will secrete GFP. Chassis is E.coli with BAC vector fragment (SGSC4024 1464540-1562427) of salmonella SPI2. Please consult papers bellow about details A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium Edward A. Miao and Samuel I. Miller 2000
mutable notes: RBS was added by primer in the process of PCR.
mutable provenance: This parts source is from genomic sequence of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. Amplified b y PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: BBa_B0034
sequence annotation: SlrP
sequence: https://synbiohub.org/public/igem/BBa_K496003_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2117351
display id: annotation2117351
version: 1
top level: BBa_K496003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K496003/annotation2117351/range2117351/1

https://synbiohub.org/public/igem/BBa_K496003/annotation2117351/range2117351/1
type: Range
persistent identity: range2117351
display id: range2117351
version: 1
direction: direction/reverse
top level: BBa_K496003
start: 1
end: 12
orientation: inline

SlrP
type: SequenceAnnotation
persistent identity: annotation2117352
display id: annotation2117352
version: 1
top level: BBa_K496003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K496003/annotation2117352/range2117352/1

https://synbiohub.org/public/igem/BBa_K496003/annotation2117352/range2117352/1
type: Range
persistent identity: range2117352
display id: range2117352
version: 1
direction: direction/forward
top level: BBa_K496003
start: 15
end: 587
orientation: inline

BBa_J36848
type: ComponentDefinition
persistent identity: BBa J36848
display id: BBa_J36848
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin wild-type + His6tag
creator: Perry Tsai
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
group u list: _65_
m user id: 0
owner id: 1229
owning group id: 65
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device contains a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type + His6 tag. This expression should display streptavidin on the cell surface of E. coli. NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts. However, this does not apply between the R0010 and B0031 in the J04500 composite because that was assembled in a standard fashion.
mutable notes: There is no spacer nucleotide between the end of the device and the SpeI restriction site. Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0031.
mutable provenance: Assembly of BBa_J04500 and BBa_J36844
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_B0034
component: BBa_J36835
component: BBa_J36836
component: BBa_J36841
sequence annotation: BBa_B0034
sequence annotation: BBa_J36836
sequence annotation: BBa_J36841
sequence annotation: BBa_J36835
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J36848_sequence/1

BBa_R0010
type: Component
persistent identity: component1909167
display id: component1909167
version: 1
top level: BBa_J36848
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component1909175
display id: component1909175
version: 1
top level: BBa_J36848
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J36835
type: Component
persistent identity: component1909176
display id: component1909176
version: 1
top level: BBa_J36848
access: public
definition: https://synbiohub.org/public/igem/BBa_J36835/1

BBa_J36836
type: Component
persistent identity: component1909177
display id: component1909177
version: 1
top level: BBa_J36848
access: public
definition: https://synbiohub.org/public/igem/BBa_J36836/1

BBa_J36841
type: Component
persistent identity: component1909178
display id: component1909178
version: 1
top level: BBa_J36848
access: public
definition: https://synbiohub.org/public/igem/BBa_J36841/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1909175
display id: annotation1909175
version: 1
top level: BBa_J36848
location: https://synbiohub.org/public/igem/BBa_J36848/annotation1909175/range1909175/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J36848/annotation1909175/range1909175/1
type: Range
persistent identity: range1909175
display id: range1909175
version: 1
direction: direction/reverse
top level: BBa_J36848
start: 209
end: 220
orientation: inline

BBa_J36836
type: SequenceAnnotation
persistent identity: annotation1909177
display id: annotation1909177
version: 1
top level: BBa_J36848
location: https://synbiohub.org/public/igem/BBa_J36848/annotation1909177/range1909177/1
component: BBa_J36836

https://synbiohub.org/public/igem/BBa_J36848/annotation1909177/range1909177/1
type: Range
persistent identity: range1909177
display id: range1909177
version: 1
direction: direction/reverse
top level: BBa_J36848
start: 322
end: 384
orientation: inline

BBa_J36841
type: SequenceAnnotation
persistent identity: annotation1909178
display id: annotation1909178
version: 1
top level: BBa_J36848
location: https://synbiohub.org/public/igem/BBa_J36848/annotation1909178/range1909178/1
component: BBa_J36841

https://synbiohub.org/public/igem/BBa_J36848/annotation1909178/range1909178/1
type: Range
persistent identity: range1909178
display id: range1909178
version: 1
direction: direction/reverse
top level: BBa_J36848
start: 393
end: 797
orientation: inline

BBa_J36835
type: SequenceAnnotation
persistent identity: annotation1909176
display id: annotation1909176
version: 1
top level: BBa_J36848
location: https://synbiohub.org/public/igem/BBa_J36848/annotation1909176/range1909176/1
component: BBa_J36835

https://synbiohub.org/public/igem/BBa_J36848/annotation1909176/range1909176/1
type: Range
persistent identity: range1909176
display id: range1909176
version: 1
direction: direction/reverse
top level: BBa_J36848
start: 227
end: 313
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1909167
display id: annotation1909167
version: 1
top level: BBa_J36848
location: https://synbiohub.org/public/igem/BBa_J36848/annotation1909167/range1909167/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J36848/annotation1909167/range1909167/1
type: Range
persistent identity: range1909167
display id: range1909167
version: 1
direction: direction/forward
top level: BBa_J36848
start: 1
end: 200
orientation: inline

VanBP
type: ComponentDefinition
persistent identity: BBa J58105
display id: BBa_J58105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synthetic periplasmic binding protein that docks a vanillin molecule
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J58105_sequence/1

BBa_K1051258
type: ComponentDefinition
persistent identity: BBa K1051258
display id: BBa_K1051258
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Construct the measurement pathway of K1051207.
creator: Rui Guan
created: 2013-09-01T11:00:00Z
modified: 2015-05-08T01:08:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 14515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Construct the final pathway with M0051
mutable notes: Construct the final pathway with M0051
mutable provenance: Construct the final pathway with M0051
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1051000
component: BBa_J04500
component: BBa_K1051207
sequence annotation: BBa_K1051000
sequence annotation: BBa_J04500
sequence annotation: BBa_K1051207
sequence: https://synbiohub.org/public/igem/BBa_K1051258_sequence/1

BBa_K1051000
type: Component
persistent identity: component2362666
display id: component2362666
version: 1
top level: BBa_K1051258
access: public
definition: BBa_K1051000

BBa_J04500
type: Component
persistent identity: component2362665
display id: component2362665
version: 1
top level: BBa_K1051258
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1051207
type: Component
persistent identity: component2362667
display id: component2362667
version: 1
top level: BBa_K1051258
access: public
definition: https://synbiohub.org/public/igem/BBa_K1051207/1

BBa_K1051000
type: SequenceAnnotation
persistent identity: annotation2362666
display id: annotation2362666
version: 1
top level: BBa_K1051258
location: https://synbiohub.org/public/igem/BBa_K1051258/annotation2362666/range2362666/1
component: BBa_K1051000

https://synbiohub.org/public/igem/BBa_K1051258/annotation2362666/range2362666/1
type: Range
persistent identity: range2362666
display id: range2362666
version: 1
direction: direction/reverse
top level: BBa_K1051258
start: 227
end: 901
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2362665
display id: annotation2362665
version: 1
top level: BBa_K1051258
location: https://synbiohub.org/public/igem/BBa_K1051258/annotation2362665/range2362665/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1051258/annotation2362665/range2362665/1
type: Range
persistent identity: range2362665
display id: range2362665
version: 1
direction: direction/reverse
top level: BBa_K1051258
start: 1
end: 220
orientation: inline

BBa_K1051207
type: SequenceAnnotation
persistent identity: annotation2362667
display id: annotation2362667
version: 1
top level: BBa_K1051258
location: https://synbiohub.org/public/igem/BBa_K1051258/annotation2362667/range2362667/1
component: BBa_K1051207

https://synbiohub.org/public/igem/BBa_K1051258/annotation2362667/range2362667/1
type: Range
persistent identity: range2362667
display id: range2362667
version: 1
direction: direction/reverse
top level: BBa_K1051258
start: 910
end: 954
orientation: inline

BBa_K554005
type: ComponentDefinition
persistent identity: BBa K554005
display id: BBa_K554005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IL12
creator: UNICAMP EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: IL12 is ...
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IL12
sequence annotation: RBS
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K554005_sequence/1

IL12
type: SequenceAnnotation
persistent identity: annotation2136884
display id: annotation2136884
version: 1
top level: BBa_K554005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K554005/annotation2136884/range2136884/1

https://synbiohub.org/public/igem/BBa_K554005/annotation2136884/range2136884/1
type: Range
persistent identity: range2136884
display id: range2136884
version: 1
direction: direction/forward
top level: BBa_K554005
start: 16
end: 1610
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136883
display id: annotation2136883
version: 1
top level: BBa_K554005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K554005/annotation2136883/range2136883/1

https://synbiohub.org/public/igem/BBa_K554005/annotation2136883/range2136883/1
type: Range
persistent identity: range2136883
display id: range2136883
version: 1
direction: direction/forward
top level: BBa_K554005
start: 1
end: 15
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2136885
display id: annotation2136885
version: 1
top level: BBa_K554005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K554005/annotation2136885/range2136885/1

https://synbiohub.org/public/igem/BBa_K554005/annotation2136885/range2136885/1
type: Range
persistent identity: range2136885
display id: range2136885
version: 1
direction: direction/forward
top level: BBa_K554005
start: 1611
end: 1617
orientation: inline

BBa_K415000
type: ComponentDefinition
persistent identity: BBa K415000
display id: BBa_K415000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/cI-OR : RBS-mCherry : Term
creator: Grant Robinson, Andrew Yang
created: 2010-06-06T11:00:00Z
modified: 2015-05-08T01:12:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _525_
m user id: 0
owner id: 6719
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a BioBrick composed of parts BBa_R0065 and BBa_J06702. This composite produces the mCherry fluorescent protein. It is activated by the presence of LuxR and AHL, and it is repressed by cI.
mutable notes: No design considerations to note.
mutable provenance: http://partsregistry.org/wiki/index.php?title=Part:BBa_R0065 http://partsregistry.org/wiki/index.php?title=Part:BBa_J06702
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J06504
component: BBa_B0034
component: BBa_R0065
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J06504
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_K415000_sequence/1

BBa_J06504
type: Component
persistent identity: component2070314
display id: component2070314
version: 1
top level: BBa_K415000
access: public
definition: https://synbiohub.org/public/igem/BBa_J06504/1

BBa_B0034
type: Component
persistent identity: component2070311
display id: component2070311
version: 1
top level: BBa_K415000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0065
type: Component
persistent identity: component2070302
display id: component2070302
version: 1
top level: BBa_K415000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0012
type: Component
persistent identity: component2070317
display id: component2070317
version: 1
top level: BBa_K415000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2070315
display id: component2070315
version: 1
top level: BBa_K415000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2070311
display id: annotation2070311
version: 1
top level: BBa_K415000
location: https://synbiohub.org/public/igem/BBa_K415000/annotation2070311/range2070311/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K415000/annotation2070311/range2070311/1
type: Range
persistent identity: range2070311
display id: range2070311
version: 1
direction: direction/reverse
top level: BBa_K415000
start: 106
end: 117
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation2070314
display id: annotation2070314
version: 1
top level: BBa_K415000
location: https://synbiohub.org/public/igem/BBa_K415000/annotation2070314/range2070314/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_K415000/annotation2070314/range2070314/1
type: Range
persistent identity: range2070314
display id: range2070314
version: 1
direction: direction/reverse
top level: BBa_K415000
start: 124
end: 837
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2070315
display id: annotation2070315
version: 1
top level: BBa_K415000
location: https://synbiohub.org/public/igem/BBa_K415000/annotation2070315/range2070315/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K415000/annotation2070315/range2070315/1
type: Range
persistent identity: range2070315
display id: range2070315
version: 1
direction: direction/forward
top level: BBa_K415000
start: 846
end: 925
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2070317
display id: annotation2070317
version: 1
top level: BBa_K415000
location: https://synbiohub.org/public/igem/BBa_K415000/annotation2070317/range2070317/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K415000/annotation2070317/range2070317/1
type: Range
persistent identity: range2070317
display id: range2070317
version: 1
direction: direction/forward
top level: BBa_K415000
start: 934
end: 974
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation2070302
display id: annotation2070302
version: 1
top level: BBa_K415000
location: https://synbiohub.org/public/igem/BBa_K415000/annotation2070302/range2070302/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_K415000/annotation2070302/range2070302/1
type: Range
persistent identity: range2070302
display id: range2070302
version: 1
direction: direction/forward
top level: BBa_K415000
start: 1
end: 97
orientation: inline

BBa_I718004
type: ComponentDefinition
persistent identity: BBa I718004
display id: BBa_I718004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DapA Coli MG1655
creator: David Bikard
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Dihydrodipicolinate synthase is the first enzyme unique to lysine biosynthesis, catalyzing the condensation of pyruvate and aspartate β-semialdehyde by a ping-pong mechanism. It also catalyzes the rate-limiting step in E.coli lysine biosynthesis after aspartate kinase III. The condensing enzyme-pyruvate complex, prior to reduction and hydrolysis, exists as the Schiff base formed between the carbonyl of pyruvate and an ε-amino group of a lysine residue on the enzyme. This property classifies the enzyme as a Class I aldolase with respect to carbon-carbon bond formation. The enzyme is inhibited by lysine, a feedback inhibitor. (source: Ecocyc)
mutable notes: none
mutable provenance: E. Coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: DapA
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I718004_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1939202
display id: annotation1939202
version: 1
top level: BBa_I718004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I718004/annotation1939202/range1939202/1

https://synbiohub.org/public/igem/BBa_I718004/annotation1939202/range1939202/1
type: Range
persistent identity: range1939202
display id: range1939202
version: 1
direction: direction/forward
top level: BBa_I718004
start: 877
end: 882
orientation: inline

DapA
type: SequenceAnnotation
persistent identity: annotation1939203
display id: annotation1939203
version: 1
top level: BBa_I718004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I718004/annotation1939203/range1939203/1

https://synbiohub.org/public/igem/BBa_I718004/annotation1939203/range1939203/1
type: Range
persistent identity: range1939203
display id: range1939203
version: 1
direction: direction/forward
top level: BBa_I718004
start: 1
end: 882
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1939201
display id: annotation1939201
version: 1
top level: BBa_I718004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I718004/annotation1939201/range1939201/1

https://synbiohub.org/public/igem/BBa_I718004/annotation1939201/range1939201/1
type: Range
persistent identity: range1939201
display id: range1939201
version: 1
direction: direction/forward
top level: BBa_I718004
start: 1
end: 3
orientation: inline

Intein
type: ComponentDefinition
persistent identity: BBa K300004
display id: BBa_K300004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Engineered pH-inducible intein (codon optimized for E. coli) - internal domain
creator: Sara Bisi, Paolo Magni
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: Codon optimized for E. coli. The amino acid sequence comes from [1].
mutable provenance: The original sequence comes from M. tuberculosis genomic DNA (RecA gene), but it has been highly engineered (according to [1]) to create a pH-inducible protein domain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K300004_sequence/1

BBa_K566015
type: ComponentDefinition
persistent identity: BBa K566015
display id: BBa_K566015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRM-RBS-cI434
creator: Daniel Rodriguez
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Composite part for Biphasic switch tests.
mutable notes: Search for illegal sites was performed, they were found and deleted.
mutable provenance: Synthesized DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K566001
component: BBa_K566011
sequence annotation: BBa_B0034
sequence annotation: BBa_K566001
sequence annotation: BBa_K566011
sequence: https://synbiohub.org/public/igem/BBa_K566015_sequence/1

BBa_B0034
type: Component
persistent identity: component2146403
display id: component2146403
version: 1
top level: BBa_K566015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K566001
type: Component
persistent identity: component2146401
display id: component2146401
version: 1
top level: BBa_K566015
access: public
definition: https://synbiohub.org/public/igem/BBa_K566001/1

BBa_B0034
type: Component
persistent identity: component2146408
display id: component2146408
version: 1
top level: BBa_K566015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K566011
type: Component
persistent identity: component2146406
display id: component2146406
version: 1
top level: BBa_K566015
access: public
definition: https://synbiohub.org/public/igem/BBa_K566011/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2146403
display id: annotation2146403
version: 1
top level: BBa_K566015
location: https://synbiohub.org/public/igem/BBa_K566015/annotation2146403/range2146403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K566015/annotation2146403/range2146403/1
type: Range
persistent identity: range2146403
display id: range2146403
version: 1
direction: direction/reverse
top level: BBa_K566015
start: 94
end: 105
orientation: inline

BBa_K566001
type: SequenceAnnotation
persistent identity: annotation2146401
display id: annotation2146401
version: 1
top level: BBa_K566015
location: https://synbiohub.org/public/igem/BBa_K566015/annotation2146401/range2146401/1
component: BBa_K566001

https://synbiohub.org/public/igem/BBa_K566015/annotation2146401/range2146401/1
type: Range
persistent identity: range2146401
display id: range2146401
version: 1
direction: direction/reverse
top level: BBa_K566015
start: 1
end: 85
orientation: inline

BBa_K566011
type: SequenceAnnotation
persistent identity: annotation2146406
display id: annotation2146406
version: 1
top level: BBa_K566015
location: https://synbiohub.org/public/igem/BBa_K566015/annotation2146406/range2146406/1
component: BBa_K566011

https://synbiohub.org/public/igem/BBa_K566015/annotation2146406/range2146406/1
type: Range
persistent identity: range2146406
display id: range2146406
version: 1
direction: direction/reverse
top level: BBa_K566015
start: 112
end: 780
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2146408
display id: annotation2146408
version: 1
top level: BBa_K566015
location: https://synbiohub.org/public/igem/BBa_K566015/annotation2146408/range2146408/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K566015/annotation2146408/range2146408/1
type: Range
persistent identity: range2146408
display id: range2146408
version: 1
direction: direction/reverse
top level: BBa_K566015
start: 789
end: 800
orientation: inline

BBa_J52642
type: ComponentDefinition
persistent identity: BBa J52642
display id: BBa_J52642
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green Flourescence Protein
creator: Jernej Kovač
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 852
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding sequence of GFP reporter protein
mutable notes: none
mutable provenance: many
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J52642_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1903213
display id: annotation1903213
version: 1
top level: BBa_J52642
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52642/annotation1903213/range1903213/1

https://synbiohub.org/public/igem/BBa_J52642/annotation1903213/range1903213/1
type: Range
persistent identity: range1903213
display id: range1903213
version: 1
direction: direction/forward
top level: BBa_J52642
start: 4
end: 716
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1903206
display id: annotation1903206
version: 1
top level: BBa_J52642
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J52642/annotation1903206/range1903206/1

https://synbiohub.org/public/igem/BBa_J52642/annotation1903206/range1903206/1
type: Range
persistent identity: range1903206
display id: range1903206
version: 1
direction: direction/forward
top level: BBa_J52642
start: 717
end: 720
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1903205
display id: annotation1903205
version: 1
top level: BBa_J52642
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J52642/annotation1903205/range1903205/1

https://synbiohub.org/public/igem/BBa_J52642/annotation1903205/range1903205/1
type: Range
persistent identity: range1903205
display id: range1903205
version: 1
direction: direction/forward
top level: BBa_J52642
start: 1
end: 3
orientation: inline

lsrR
type: ComponentDefinition
persistent identity: BBa K091001
display id: BBa_K091001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrR gene
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This sequence codes for the LsrR gene which is found in the E. coli strain MG1655. When this protein fors a complex with phosphorylated AI-2 it promotes the LsrA promoter.
mutable notes: no special considerations
mutable provenance: The DNA for this part is found in the E. coli strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091001_sequence/1

Bt-ALDH
type: ComponentDefinition
persistent identity: BBa K398006
display id: BBa_K398006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium-Chain Aldehyde Dehydrogenase
creator: Mathias Voges
created: 2010-06-12T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6320
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Aldehyde dehydrogenase from Geobacillus thermoleovorans B23.
mutable notes: Nucleotide sequence optimized for expression in E.coli.
mutable provenance: Genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: Bt-ALDH
sequence: https://synbiohub.org/public/igem/BBa_K398006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2070756
display id: annotation2070756
version: 1
top level: Bt-ALDH
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K398006/annotation2070756/range2070756/1

https://synbiohub.org/public/igem/BBa_K398006/annotation2070756/range2070756/1
type: Range
persistent identity: range2070756
display id: range2070756
version: 1
direction: direction/forward
top level: Bt-ALDH
start: 1492
end: 1494
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2070755
display id: annotation2070755
version: 1
top level: Bt-ALDH
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K398006/annotation2070755/range2070755/1

https://synbiohub.org/public/igem/BBa_K398006/annotation2070755/range2070755/1
type: Range
persistent identity: range2070755
display id: range2070755
version: 1
direction: direction/forward
top level: Bt-ALDH
start: 1
end: 3
orientation: inline

Bt-ALDH
type: SequenceAnnotation
persistent identity: annotation2070757
display id: annotation2070757
version: 1
top level: Bt-ALDH
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K398006/annotation2070757/range2070757/1

https://synbiohub.org/public/igem/BBa_K398006/annotation2070757/range2070757/1
type: Range
persistent identity: range2070757
display id: range2070757
version: 1
direction: direction/forward
top level: Bt-ALDH
start: 1
end: 1494
orientation: inline

BBa_P10000
type: ComponentDefinition
persistent identity: BBa P10000
display id: BBa_P10000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA promoter with 5' UTR
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:38:13Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10000_sequence/1

BBa_K902019
type: ComponentDefinition
persistent identity: BBa K902019
display id: BBa_K902019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S7 micrococcal nuclease
creator: Himika Dastidar
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1167_
m user id: 0
owner id: 13453
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: S7 micrococcal nuclease
mutable notes: It had to be mutated
mutable provenance: This part was synthesized from IDT and is native to Staphylococcus aureus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: See Design Considerations
sequence: https://synbiohub.org/public/igem/BBa_K902019_sequence/1

See Design Considerations
type: SequenceAnnotation
persistent identity: annotation2211172
display id: annotation2211172
version: 1
top level: BBa_K902019
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K902019/annotation2211172/range2211172/1

https://synbiohub.org/public/igem/BBa_K902019/annotation2211172/range2211172/1
type: Range
persistent identity: range2211172
display id: range2211172
version: 1
direction: direction/forward
top level: BBa_K902019
start: 312
end: 312
orientation: inline

BBa_K912000
type: ComponentDefinition
persistent identity: BBa K912000
display id: BBa_K912000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Citrobacter braakii phytase
creator: Grant Nicholas
created: 2012-07-08T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1177_
m user id: 0
owner id: 12923
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Part to produce C. braakii phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.
mutable notes: In progress.
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AY471611.1 Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: protein
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K912000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2210373
display id: annotation2210373
version: 1
top level: BBa_K912000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K912000/annotation2210373/range2210373/1

https://synbiohub.org/public/igem/BBa_K912000/annotation2210373/range2210373/1
type: Range
persistent identity: range2210373
display id: range2210373
version: 1
direction: direction/forward
top level: BBa_K912000
start: 1
end: 3
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation2210374
display id: annotation2210374
version: 1
top level: BBa_K912000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K912000/annotation2210374/range2210374/1

https://synbiohub.org/public/igem/BBa_K912000/annotation2210374/range2210374/1
type: Range
persistent identity: range2210374
display id: range2210374
version: 1
direction: direction/forward
top level: BBa_K912000
start: 1
end: 1302
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2210375
display id: annotation2210375
version: 1
top level: BBa_K912000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K912000/annotation2210375/range2210375/1

https://synbiohub.org/public/igem/BBa_K912000/annotation2210375/range2210375/1
type: Range
persistent identity: range2210375
display id: range2210375
version: 1
direction: direction/forward
top level: BBa_K912000
start: 1300
end: 1302
orientation: inline

BBa_K358006
type: ComponentDefinition
persistent identity: BBa K358006
display id: BBa_K358006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda lysis cassette with terminator
creator: Ken Kajita
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lambda lysis cassette[BBa_K358004] with double terminator[BBa_B0015].
mutable notes: non
mutable provenance: BBa_K358004 and BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K358004
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_K358004
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K358006_sequence/1

BBa_K358004
type: Component
persistent identity: component2080471
display id: component2080471
version: 1
top level: BBa_K358006
access: public
definition: https://synbiohub.org/public/igem/BBa_K358004/1

BBa_B0012
type: Component
persistent identity: component2080474
display id: component2080474
version: 1
top level: BBa_K358006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2080472
display id: component2080472
version: 1
top level: BBa_K358006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2080472
display id: annotation2080472
version: 1
top level: BBa_K358006
location: https://synbiohub.org/public/igem/BBa_K358006/annotation2080472/range2080472/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K358006/annotation2080472/range2080472/1
type: Range
persistent identity: range2080472
display id: range2080472
version: 1
direction: direction/forward
top level: BBa_K358006
start: 1282
end: 1361
orientation: inline

BBa_K358004
type: SequenceAnnotation
persistent identity: annotation2080471
display id: annotation2080471
version: 1
top level: BBa_K358006
location: https://synbiohub.org/public/igem/BBa_K358006/annotation2080471/range2080471/1
component: BBa_K358004

https://synbiohub.org/public/igem/BBa_K358006/annotation2080471/range2080471/1
type: Range
persistent identity: range2080471
display id: range2080471
version: 1
direction: direction/reverse
top level: BBa_K358006
start: 1
end: 1273
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2080474
display id: annotation2080474
version: 1
top level: BBa_K358006
location: https://synbiohub.org/public/igem/BBa_K358006/annotation2080474/range2080474/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K358006/annotation2080474/range2080474/1
type: Range
persistent identity: range2080474
display id: range2080474
version: 1
direction: direction/forward
top level: BBa_K358006
start: 1370
end: 1410
orientation: inline

BBa_K317030
type: ComponentDefinition
persistent identity: BBa K317030
display id: BBa_K317030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(J23102)-RBS(B0034)-pduP_GFP-Term(B0015)
creator: Yoshiyuki MURAKAMI
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:11:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _436_
m user id: 0
owner id: 4686
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: this part is chimeric protein of the enzyme relating propandiol metabolize and green fluorescent protein. this part was localize to BMC.
mutable notes: none
mutable provenance: psudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_J23102
sequence annotation: pduP-GFPuv fusion
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K317030_sequence/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2109379
display id: annotation2109379
version: 1
top level: BBa_K317030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K317030/annotation2109379/range2109379/1

https://synbiohub.org/public/igem/BBa_K317030/annotation2109379/range2109379/1
type: Range
persistent identity: range2109379
display id: range2109379
version: 1
direction: direction/reverse
top level: BBa_K317030
start: 1
end: 35
orientation: inline

pduP-GFPuv fusion
type: SequenceAnnotation
persistent identity: annotation2109381
display id: annotation2109381
version: 1
top level: BBa_K317030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K317030/annotation2109381/range2109381/1

https://synbiohub.org/public/igem/BBa_K317030/annotation2109381/range2109381/1
type: Range
persistent identity: range2109381
display id: range2109381
version: 1
direction: direction/forward
top level: BBa_K317030
start: 62
end: 832
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2109386
display id: annotation2109386
version: 1
top level: BBa_K317030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K317030/annotation2109386/range2109386/1

https://synbiohub.org/public/igem/BBa_K317030/annotation2109386/range2109386/1
type: Range
persistent identity: range2109386
display id: range2109386
version: 1
direction: direction/forward
top level: BBa_K317030
start: 841
end: 969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2109380
display id: annotation2109380
version: 1
top level: BBa_K317030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K317030/annotation2109380/range2109380/1

https://synbiohub.org/public/igem/BBa_K317030/annotation2109380/range2109380/1
type: Range
persistent identity: range2109380
display id: range2109380
version: 1
direction: direction/reverse
top level: BBa_K317030
start: 44
end: 55
orientation: inline

BBa_K398030
type: ComponentDefinition
persistent identity: BBa K398030
display id: BBa_K398030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ALDH generator (over-expression)
creator: Kira Schipper
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6322
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ALDH, an aldehyde deyhydrogenase that facilitates the third step in alkane degradation, from n-alkanals to n-alkanoic acids, which can then be further degraded through β-oxidation
mutable notes: Nucleotide sequence optimized for expression in E.coli
mutable provenance: Genebank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K398006
component: BBa_B0034
sequence annotation: BBa_K398006
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K398030_sequence/1

BBa_R0040
type: Component
persistent identity: component2083587
display id: component2083587
version: 1
top level: BBa_K398030
access: public
definition: p(tetR)

BBa_K398006
type: Component
persistent identity: component2083597
display id: component2083597
version: 1
top level: BBa_K398030
access: public
definition: Bt-ALDH

BBa_B0034
type: Component
persistent identity: component2083593
display id: component2083593
version: 1
top level: BBa_K398030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K398006
type: SequenceAnnotation
persistent identity: annotation2083597
display id: annotation2083597
version: 1
top level: BBa_K398030
location: https://synbiohub.org/public/igem/BBa_K398030/annotation2083597/range2083597/1
component: BBa_K398006

https://synbiohub.org/public/igem/BBa_K398030/annotation2083597/range2083597/1
type: Range
persistent identity: range2083597
display id: range2083597
version: 1
direction: direction/reverse
top level: BBa_K398030
start: 81
end: 1574
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2083593
display id: annotation2083593
version: 1
top level: BBa_K398030
location: https://synbiohub.org/public/igem/BBa_K398030/annotation2083593/range2083593/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K398030/annotation2083593/range2083593/1
type: Range
persistent identity: range2083593
display id: range2083593
version: 1
direction: direction/reverse
top level: BBa_K398030
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2083587
display id: annotation2083587
version: 1
top level: BBa_K398030
location: https://synbiohub.org/public/igem/BBa_K398030/annotation2083587/range2083587/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K398030/annotation2083587/range2083587/1
type: Range
persistent identity: range2083587
display id: range2083587
version: 1
direction: direction/forward
top level: BBa_K398030
start: 1
end: 54
orientation: inline

BBa_K864402
type: ComponentDefinition
persistent identity: BBa K864402
display id: BBa_K864402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23110-B0034-eforRed
creator: Erik Lundin
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23110-B0034-eforRed
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23110
component: BBa_B0034
component: BBa_K592012
sequence annotation: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_K592012
sequence: https://synbiohub.org/public/igem/BBa_K864402_sequence/1

BBa_J23110
type: Component
persistent identity: component2213813
display id: component2213813
version: 1
top level: BBa_K864402
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0034
type: Component
persistent identity: component2213815
display id: component2213815
version: 1
top level: BBa_K864402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592012
type: Component
persistent identity: component2213817
display id: component2213817
version: 1
top level: BBa_K864402
access: public
definition: eforRed

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2213815
display id: annotation2213815
version: 1
top level: BBa_K864402
location: https://synbiohub.org/public/igem/BBa_K864402/annotation2213815/range2213815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K864402/annotation2213815/range2213815/1
type: Range
persistent identity: range2213815
display id: range2213815
version: 1
direction: direction/reverse
top level: BBa_K864402
start: 44
end: 55
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2213813
display id: annotation2213813
version: 1
top level: BBa_K864402
location: https://synbiohub.org/public/igem/BBa_K864402/annotation2213813/range2213813/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K864402/annotation2213813/range2213813/1
type: Range
persistent identity: range2213813
display id: range2213813
version: 1
direction: direction/reverse
top level: BBa_K864402
start: 1
end: 35
orientation: inline

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2213817
display id: annotation2213817
version: 1
top level: BBa_K864402
location: https://synbiohub.org/public/igem/BBa_K864402/annotation2213817/range2213817/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K864402/annotation2213817/range2213817/1
type: Range
persistent identity: range2213817
display id: range2213817
version: 1
direction: direction/reverse
top level: BBa_K864402
start: 62
end: 742
orientation: inline

BBa_K094100
type: ComponentDefinition
persistent identity: BBa K094100
display id: BBa_K094100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ gene
creator: Liu Chenli
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the cheZ operon coding region without an rbs nor a terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
mutable notes: This part is without promoter, rbs or terminator.
mutable provenance: The origin of the cheZ gene comes from E. Coli strain MG1655 chromosome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cheZ
sequence: https://synbiohub.org/public/igem/BBa_K094100_sequence/1

cheZ
type: SequenceAnnotation
persistent identity: annotation1980478
display id: annotation1980478
version: 1
top level: BBa_K094100
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K094100/annotation1980478/range1980478/1

https://synbiohub.org/public/igem/BBa_K094100/annotation1980478/range1980478/1
type: Range
persistent identity: range1980478
display id: range1980478
version: 1
direction: direction/forward
top level: BBa_K094100
start: 1
end: 645
orientation: inline

RFC 37
type: ComponentDefinition
persistent identity: BBa K245050
display id: BBa_K245050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GyrB-CutA1
creator: ??pela Miklavič
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _337_
m user id: 0
owner id: 4969
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K245009
component: BBa_K245129
component: BBa_K245116
sequence annotation: BBa_K245009
sequence annotation: BBa_K245116
sequence annotation: BBa_K245129
sequence: https://synbiohub.org/public/igem/BBa_K245050_sequence/1

BBa_K245009
type: Component
persistent identity: component2061223
display id: component2061223
version: 1
top level: RFC 37
access: public
definition: https://synbiohub.org/public/igem/BBa_K245009/1

BBa_K245129
type: Component
persistent identity: component2061224
display id: component2061224
version: 1
top level: RFC 37
access: public
definition: https://synbiohub.org/public/igem/BBa_K245129/1

BBa_K245116
type: Component
persistent identity: component2061221
display id: component2061221
version: 1
top level: RFC 37
access: public
definition: https://synbiohub.org/public/igem/BBa_K245116/1

BBa_K245009
type: SequenceAnnotation
persistent identity: annotation2061223
display id: annotation2061223
version: 1
top level: RFC 37
location: https://synbiohub.org/public/igem/BBa_K245050/annotation2061223/range2061223/1
component: BBa_K245009

https://synbiohub.org/public/igem/BBa_K245050/annotation2061223/range2061223/1
type: Range
persistent identity: range2061223
display id: range2061223
version: 1
direction: direction/reverse
top level: RFC 37
start: 661
end: 666
orientation: inline

BBa_K245116
type: SequenceAnnotation
persistent identity: annotation2061221
display id: annotation2061221
version: 1
top level: RFC 37
location: https://synbiohub.org/public/igem/BBa_K245050/annotation2061221/range2061221/1
component: BBa_K245116

https://synbiohub.org/public/igem/BBa_K245050/annotation2061221/range2061221/1
type: Range
persistent identity: range2061221
display id: range2061221
version: 1
direction: direction/reverse
top level: RFC 37
start: 1
end: 660
orientation: inline

BBa_K245129
type: SequenceAnnotation
persistent identity: annotation2061224
display id: annotation2061224
version: 1
top level: RFC 37
location: https://synbiohub.org/public/igem/BBa_K245050/annotation2061224/range2061224/1
component: BBa_K245129

https://synbiohub.org/public/igem/BBa_K245050/annotation2061224/range2061224/1
type: Range
persistent identity: range2061224
display id: range2061224
version: 1
direction: direction/reverse
top level: RFC 37
start: 667
end: 999
orientation: inline

GluColi
type: ComponentDefinition
persistent identity: BBa K196014
display id: BBa_K196014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Final GluColi device.
creator: Laetitia Warny
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the part we actually created to test the glue production by E. coli. HfsG and HfsH come from Caulobacter crescentu. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. RFP has been added as a reporter.
mutable notes: As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by GeneArt [2]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.
mutable provenance: HfsG and HfsH sequences come from Caulobacter crescentus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K196005
component: BBa_K196007
sequence annotation: BBa_K196007
sequence annotation: BBa_K196005
sequence: https://synbiohub.org/public/igem/BBa_K196014_sequence/1

BBa_K196005
type: Component
persistent identity: component2267888
display id: component2267888
version: 1
top level: GluColi
access: public
definition: https://synbiohub.org/public/igem/BBa_K196005/1

BBa_K196007
type: Component
persistent identity: component2267879
display id: component2267879
version: 1
top level: GluColi
access: public
definition: https://synbiohub.org/public/igem/BBa_K196007/1

BBa_K196007
type: SequenceAnnotation
persistent identity: annotation2267879
display id: annotation2267879
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
component: BBa_K196007

https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
type: Range
persistent identity: range2267879
display id: range2267879
version: 1
direction: direction/reverse
top level: GluColi
start: 1
end: 787
orientation: inline

BBa_K196005
type: SequenceAnnotation
persistent identity: annotation2267888
display id: annotation2267888
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
component: BBa_K196005

https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
type: Range
persistent identity: range2267888
display id: range2267888
version: 1
direction: direction/reverse
top level: GluColi
start: 794
end: 2646
orientation: inline

spxB
type: ComponentDefinition
persistent identity: BBa K137069
display id: BBa_K137069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pyruvate oxidase (SpxB)
creator: Doug Tischer
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the pyruvate oxidase from Streptococcus pneumoniae R6. It catalyzes the formation of hydrogen peroxide by oxidizing pyruvate in the reaction: pyruvate + Pi + O2 --> acetyl-P + CO2 + H2O2.
mutable notes: Removal of a PstI site very near the 5' end with PCR primers.
mutable provenance: Genomic PCR from a prepared genome of Streptococcus pneumoniae R6 from ATCC (# BAA-255D-5)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T->G
sequence: https://synbiohub.org/public/igem/BBa_K137069_sequence/1

T->G
type: SequenceAnnotation
persistent identity: annotation1969567
display id: annotation1969567
version: 1
top level: spxB
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K137069/annotation1969567/range1969567/1

https://synbiohub.org/public/igem/BBa_K137069/annotation1969567/range1969567/1
type: Range
persistent identity: range1969567
display id: range1969567
version: 1
direction: direction/forward
top level: spxB
start: 27
end: 27
orientation: inline

ARL2A0201
type: ComponentDefinition
persistent identity: BBa I732109
display id: BBa_I732109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0201
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732109_sequence/1

BBa_P10200
type: ComponentDefinition
persistent identity: BBa P10200
display id: BBa_P10200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: omega tobacco mosaic virus
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:50:22Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP41402
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10200_sequence/1

BBa_K634002
type: ComponentDefinition
persistent identity: BBa K634002
display id: BBa_K634002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: exaDE
creator: Kenneth O. Xu
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:57Z
discontinued: false
dominant: false
group u list: _809_
m user id: 0
owner id: 9393
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The 2 component system that responds to ethanol that activates transcription of pexaA.
mutable notes: Each part contains a RBS but the part does not contain a promoter.
mutable provenance: Cloning of k634000 and k634001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K634000
component: BBa_K634001
sequence annotation: BBa_K634001
sequence annotation: BBa_K634000
sequence: https://synbiohub.org/public/igem/BBa_K634002_sequence/1

BBa_K634000
type: Component
persistent identity: component2129709
display id: component2129709
version: 1
top level: BBa_K634002
access: public
definition: https://synbiohub.org/public/igem/BBa_K634000/1

BBa_K634001
type: Component
persistent identity: component2129712
display id: component2129712
version: 1
top level: BBa_K634002
access: public
definition: https://synbiohub.org/public/igem/BBa_K634001/1

BBa_K634001
type: SequenceAnnotation
persistent identity: annotation2129712
display id: annotation2129712
version: 1
top level: BBa_K634002
location: https://synbiohub.org/public/igem/BBa_K634002/annotation2129712/range2129712/1
component: BBa_K634001

https://synbiohub.org/public/igem/BBa_K634002/annotation2129712/range2129712/1
type: Range
persistent identity: range2129712
display id: range2129712
version: 1
direction: direction/reverse
top level: BBa_K634002
start: 850
end: 1541
orientation: inline

BBa_K634000
type: SequenceAnnotation
persistent identity: annotation2129709
display id: annotation2129709
version: 1
top level: BBa_K634002
location: https://synbiohub.org/public/igem/BBa_K634002/annotation2129709/range2129709/1
component: BBa_K634000

https://synbiohub.org/public/igem/BBa_K634002/annotation2129709/range2129709/1
type: Range
persistent identity: range2129709
display id: range2129709
version: 1
direction: direction/reverse
top level: BBa_K634002
start: 1
end: 841
orientation: inline

BBa_K1060009
type: ComponentDefinition
persistent identity: BBa K1060009
display id: BBa_K1060009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium constitutive expression of EBF synthase from Artemisia annua
creator: Ingmar Claes, Su Wang, Sylvie Weckx
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1368_
m user id: 0
owner id: 17634
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006).
mutable notes: no EcoRI, XbaI, NotI, SpeI or PstI sites present
mutable provenance: Composite part; genomic sequence from EBF synthase from Artemisia annua combined with iGEM bricks. BBa_K608006 + Bba_K1060002 + Bba_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1060002
component: BBa_B0015
component: BBa_K608006
sequence annotation: BBa_K608006
sequence annotation: BBa_K1060002
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1060009_sequence/1

BBa_K1060002
type: Component
persistent identity: component2353987
display id: component2353987
version: 1
top level: BBa_K1060009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1060002/1

BBa_B0015
type: Component
persistent identity: component2353994
display id: component2353994
version: 1
top level: BBa_K1060009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K608006
type: Component
persistent identity: component2353986
display id: component2353986
version: 1
top level: BBa_K1060009
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2353986
display id: annotation2353986
version: 1
top level: BBa_K1060009
location: https://synbiohub.org/public/igem/BBa_K1060009/annotation2353986/range2353986/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K1060009/annotation2353986/range2353986/1
type: Range
persistent identity: range2353986
display id: range2353986
version: 1
direction: direction/reverse
top level: BBa_K1060009
start: 1
end: 56
orientation: inline

BBa_K1060002
type: SequenceAnnotation
persistent identity: annotation2353987
display id: annotation2353987
version: 1
top level: BBa_K1060009
location: https://synbiohub.org/public/igem/BBa_K1060009/annotation2353987/range2353987/1
component: BBa_K1060002

https://synbiohub.org/public/igem/BBa_K1060009/annotation2353987/range2353987/1
type: Range
persistent identity: range2353987
display id: range2353987
version: 1
direction: direction/reverse
top level: BBa_K1060009
start: 63
end: 1787
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2353994
display id: annotation2353994
version: 1
top level: BBa_K1060009
location: https://synbiohub.org/public/igem/BBa_K1060009/annotation2353994/range2353994/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1060009/annotation2353994/range2353994/1
type: Range
persistent identity: range2353994
display id: range2353994
version: 1
direction: direction/reverse
top level: BBa_K1060009
start: 1796
end: 1924
orientation: inline

BBa_K257002
type: ComponentDefinition
persistent identity: BBa K257002
display id: BBa_K257002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ClyA_Fusion_C-Term
creator: Vicard Du
created: 2009-08-19T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _354_
m user id: 0
owner id: 5011
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ClyA ....
mutable notes: ...
mutable provenance: Genomic sequenced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Good rbs
sequence annotation: hlyE
sequence annotation: poly Gly linker
sequence: https://synbiohub.org/public/igem/BBa_K257002_sequence/1

Good rbs
type: SequenceAnnotation
persistent identity: annotation2056561
display id: annotation2056561
version: 1
top level: BBa_K257002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K257002/annotation2056561/range2056561/1

https://synbiohub.org/public/igem/BBa_K257002/annotation2056561/range2056561/1
type: Range
persistent identity: range2056561
display id: range2056561
version: 1
direction: direction/forward
top level: BBa_K257002
start: 1
end: 8
orientation: inline

hlyE
type: SequenceAnnotation
persistent identity: annotation2056562
display id: annotation2056562
version: 1
top level: BBa_K257002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K257002/annotation2056562/range2056562/1

https://synbiohub.org/public/igem/BBa_K257002/annotation2056562/range2056562/1
type: Range
persistent identity: range2056562
display id: range2056562
version: 1
direction: direction/forward
top level: BBa_K257002
start: 14
end: 922
orientation: inline

poly Gly linker
type: SequenceAnnotation
persistent identity: annotation2056560
display id: annotation2056560
version: 1
top level: BBa_K257002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K257002/annotation2056560/range2056560/1

https://synbiohub.org/public/igem/BBa_K257002/annotation2056560/range2056560/1
type: Range
persistent identity: range2056560
display id: range2056560
version: 1
direction: direction/forward
top level: BBa_K257002
start: 923
end: 937
orientation: inline

BBa_I712028
type: ComponentDefinition
persistent identity: BBa I712028
display id: BBa_I712028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence
creator: Marko Bitenc
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1835
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This protein is used to show that T7 polymerase is actually going into the nucleolus, where it can transcribe genes under T7 promotor.
mutable notes: In the original cherry protein there was a PstI restriction site in the middle of the sequence. That is why mutagenesis of this restiction site was necessary.
mutable provenance: From one clone that Gabi gave me - will be further described in the future.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712028_sequence/1

BBa_K1051001
type: ComponentDefinition
persistent identity: BBa K1051001
display id: BBa_K1051001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stop codon free ECFP in RFC[23] standard
creator: Xiang LI
created: 2013-07-03T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15912
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ECFP without stop coding
mutable notes: add prefix and suffix del stop coding
mutable provenance: kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1051001_sequence/1

BBa_K189000
type: ComponentDefinition
persistent identity: BBa K189000
display id: BBa_K189000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Targeted BioBrick
creator: Feng Tian, Teng Li
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:13Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _290_
m user id: 0
owner id: 4058
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Targeted Biobrick is a specially constructed for cell-specific attachment and subsequent internalization. It consists of a TAT peptide for attachment (can be modified by other peptide for different specificity), the selected segment in fiber shaft of adenovirus and RGD sequence. Different modules or domains are linked by linker sequences.
mutable notes: N/A
mutable provenance: Constructed by our team
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K189000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2037731
display id: annotation2037731
version: 1
top level: BBa_K189000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K189000/annotation2037731/range2037731/1

https://synbiohub.org/public/igem/BBa_K189000/annotation2037731/range2037731/1
type: Range
persistent identity: range2037731
display id: range2037731
version: 1
direction: direction/forward
top level: BBa_K189000
start: 1
end: 1263
orientation: inline

BBa_K608018
type: ComponentDefinition
persistent identity: BBa K608018
display id: BBa_K608018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium promoter and weak RBS with RFP
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promotor,RBS with RFP
mutable notes: composite
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K608007
component: BBa_E1010
sequence annotation: BBa_K608007
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K608018_sequence/1

BBa_K608007
type: Component
persistent identity: component2247918
display id: component2247918
version: 1
top level: BBa_K608018
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_E1010
type: Component
persistent identity: component2247921
display id: component2247921
version: 1
top level: BBa_K608018
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2247918
display id: annotation2247918
version: 1
top level: BBa_K608018
location: https://synbiohub.org/public/igem/BBa_K608018/annotation2247918/range2247918/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K608018/annotation2247918/range2247918/1
type: Range
persistent identity: range2247918
display id: range2247918
version: 1
direction: direction/reverse
top level: BBa_K608018
start: 1
end: 57
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247921
display id: annotation2247921
version: 1
top level: BBa_K608018
location: https://synbiohub.org/public/igem/BBa_K608018/annotation2247921/range2247921/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K608018/annotation2247921/range2247921/1
type: Range
persistent identity: range2247921
display id: range2247921
version: 1
direction: direction/reverse
top level: BBa_K608018
start: 64
end: 769
orientation: inline

BBa_K638001
type: ComponentDefinition
persistent identity: BBa K638001
display id: BBa_K638001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reflectin A1 from Loligo
creator: Matthew Jones
created: 2011-08-03T11:00:00Z
modified: 2015-05-08T01:12:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _814_
m user id: 0
owner id: 9698
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: reflectin A1
sequence annotation: stop codon
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K638001_sequence/1

reflectin A1
type: SequenceAnnotation
persistent identity: annotation2127497
display id: annotation2127497
version: 1
top level: BBa_K638001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K638001/annotation2127497/range2127497/1

https://synbiohub.org/public/igem/BBa_K638001/annotation2127497/range2127497/1
type: Range
persistent identity: range2127497
display id: range2127497
version: 1
direction: direction/forward
top level: BBa_K638001
start: 19
end: 1077
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2127498
display id: annotation2127498
version: 1
top level: BBa_K638001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K638001/annotation2127498/range2127498/1

https://synbiohub.org/public/igem/BBa_K638001/annotation2127498/range2127498/1
type: Range
persistent identity: range2127498
display id: range2127498
version: 1
direction: direction/forward
top level: BBa_K638001
start: 1078
end: 1080
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2127496
display id: annotation2127496
version: 1
top level: BBa_K638001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K638001/annotation2127496/range2127496/1

https://synbiohub.org/public/igem/BBa_K638001/annotation2127496/range2127496/1
type: Range
persistent identity: range2127496
display id: range2127496
version: 1
direction: direction/forward
top level: BBa_K638001
start: 1
end: 12
orientation: inline

BBa_K315014
type: ComponentDefinition
persistent identity: BBa K315014
display id: BBa_K315014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetA (Optimized from bp 1 to 141)
creator: Danielle Wagner
created: 2010-07-05T11:00:00Z
modified: 2015-05-08T01:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _435_
m user id: 0
owner id: 7416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This sequence is the wild type TetA gene which has been optimized from base pairs 1 to 141. This is a basic part that can be placed behind a promoter and produce the Tetracycline resistance protein. Being that this gene is optimized, we are hypothesizing that it will allow for greater translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis).
mutable notes: During the design of this sequence, it was necessary to ensure that the optimized sequence did not contain BioBrick restriction sites and that the translational sequence was identical to that of the wild type gene.
mutable provenance: This TetA gene has been optimized from base pairs 1 to 141 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/)and was constructed using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K315014_sequence/1

BBa_K360129
type: ComponentDefinition
persistent identity: BBa K360129
display id: BBa_K360129
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117 constitutive promoter + LovTAP Photosensor
creator: Claudia Ivonne Hernandez Armenta
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _485_
m user id: 0
owner id: 6618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K360121
component: BBa_J23117
sequence annotation: BBa_K360121
sequence annotation: BBa_J23117
sequence: https://synbiohub.org/public/igem/BBa_K360129_sequence/1

BBa_K360121
type: Component
persistent identity: component2092012
display id: component2092012
version: 1
top level: BBa_K360129
access: public
definition: BBa_K360121

BBa_J23117
type: Component
persistent identity: component2092005
display id: component2092005
version: 1
top level: BBa_K360129
access: public
definition: https://synbiohub.org/public/igem/BBa_J23117/1

BBa_K360121
type: SequenceAnnotation
persistent identity: annotation2092012
display id: annotation2092012
version: 1
top level: BBa_K360129
location: https://synbiohub.org/public/igem/BBa_K360129/annotation2092012/range2092012/1
component: BBa_K360121

https://synbiohub.org/public/igem/BBa_K360129/annotation2092012/range2092012/1
type: Range
persistent identity: range2092012
display id: range2092012
version: 1
direction: direction/reverse
top level: BBa_K360129
start: 44
end: 887
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2092005
display id: annotation2092005
version: 1
top level: BBa_K360129
location: https://synbiohub.org/public/igem/BBa_K360129/annotation2092005/range2092005/1
component: BBa_J23117

https://synbiohub.org/public/igem/BBa_K360129/annotation2092005/range2092005/1
type: Range
persistent identity: range2092005
display id: range2092005
version: 1
direction: direction/reverse
top level: BBa_K360129
start: 1
end: 35
orientation: inline

BBa_K864404
type: ComponentDefinition
persistent identity: BBa K864404
display id: BBa_K864404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cjBlue Coding Device
creator: Pikkei Yuen
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9973
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: cjBlue is a blue (λ[maxabs]=610nm) chromoprotein from the sea anemone Cnidopus japoincus, as reported by (Chan et. al., 2006). We synthesized cjBlue codon optimized for expression in e.coli and observed a turquoise colour, wavelength approximately 5*10^2nm. This part is also submitted as a functionally active variant with J23110 and B0034
mutable notes: Codon-optimized for E.coli.
mutable provenance: Synthesized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23110
component: BBa_B0034
component: BBa_K592011
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_K592011
sequence: https://synbiohub.org/public/igem/BBa_K864404_sequence/1

BBa_J23110
type: Component
persistent identity: component2373057
display id: component2373057
version: 1
top level: BBa_K864404
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0034
type: Component
persistent identity: component2373059
display id: component2373059
version: 1
top level: BBa_K864404
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592011
type: Component
persistent identity: component2373061
display id: component2373061
version: 1
top level: BBa_K864404
access: public
definition: https://synbiohub.org/public/igem/BBa_K592011/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2373057
display id: annotation2373057
version: 1
top level: BBa_K864404
location: https://synbiohub.org/public/igem/BBa_K864404/annotation2373057/range2373057/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K864404/annotation2373057/range2373057/1
type: Range
persistent identity: range2373057
display id: range2373057
version: 1
direction: direction/reverse
top level: BBa_K864404
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2373059
display id: annotation2373059
version: 1
top level: BBa_K864404
location: https://synbiohub.org/public/igem/BBa_K864404/annotation2373059/range2373059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K864404/annotation2373059/range2373059/1
type: Range
persistent identity: range2373059
display id: range2373059
version: 1
direction: direction/reverse
top level: BBa_K864404
start: 44
end: 55
orientation: inline

BBa_K592011
type: SequenceAnnotation
persistent identity: annotation2373061
display id: annotation2373061
version: 1
top level: BBa_K864404
location: https://synbiohub.org/public/igem/BBa_K864404/annotation2373061/range2373061/1
component: BBa_K592011

https://synbiohub.org/public/igem/BBa_K864404/annotation2373061/range2373061/1
type: Range
persistent identity: range2373061
display id: range2373061
version: 1
direction: direction/reverse
top level: BBa_K864404
start: 62
end: 763
orientation: inline

BBa_K082004
type: ComponentDefinition
persistent identity: BBa K082004
display id: BBa_K082004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI- wild type
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The
mutable notes: The
mutable provenance: The
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K082004
sequence: https://synbiohub.org/public/igem/BBa_K082004_sequence/1

BBa_K082004
type: SequenceAnnotation
persistent identity: annotation1992777
display id: annotation1992777
version: 1
top level: BBa_K082004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082004/annotation1992777/range1992777/1

https://synbiohub.org/public/igem/BBa_K082004/annotation1992777/range1992777/1
type: Range
persistent identity: range1992777
display id: range1992777
version: 1
direction: direction/reverse
top level: BBa_K082004
start: 1
end: 1083
orientation: inline

BBa_K953000
type: ComponentDefinition
persistent identity: BBa K953000
display id: BBa_K953000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)
creator: Ryan Kenny
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1221_
m user id: 0
owner id: 14254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS) and T7 promoter ( Part BBa1719005 , oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
mutable notes: GC rich regions, which formed hairpin loops in synthesis, were removed. Restriction sites were removed to conform to biobrick standards.
mutable provenance: The part was codon optimsed (http://www.bioinformatics.org/sms2/rev_trans.html) from Synechocystis and assembled using Gibson Assembly. Select GC rich regions were removed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K953004
component: BBa_I712074
sequence annotation: BBa_I712074
sequence annotation: BBa_K953004
sequence: https://synbiohub.org/public/igem/BBa_K953000_sequence/1

BBa_K953004
type: Component
persistent identity: component2199294
display id: component2199294
version: 1
top level: BBa_K953000
access: public
definition: BBa_K953004

BBa_I712074
type: Component
persistent identity: component2199289
display id: component2199289
version: 1
top level: BBa_K953000
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2199289
display id: annotation2199289
version: 1
top level: BBa_K953000
location: https://synbiohub.org/public/igem/BBa_K953000/annotation2199289/range2199289/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K953000/annotation2199289/range2199289/1
type: Range
persistent identity: range2199289
display id: range2199289
version: 1
direction: direction/reverse
top level: BBa_K953000
start: 1
end: 46
orientation: inline

BBa_K953004
type: SequenceAnnotation
persistent identity: annotation2199294
display id: annotation2199294
version: 1
top level: BBa_K953000
location: https://synbiohub.org/public/igem/BBa_K953000/annotation2199294/range2199294/1
component: BBa_K953004

https://synbiohub.org/public/igem/BBa_K953000/annotation2199294/range2199294/1
type: Range
persistent identity: range2199294
display id: range2199294
version: 1
direction: direction/reverse
top level: BBa_K953000
start: 47
end: 794
orientation: inline

BBa_K360128
type: ComponentDefinition
persistent identity: BBa K360128
display id: BBa_K360128
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114 constitutive promoter + LovTAP Photosensor
creator: Claudia Ivonne Hernandez Armenta
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _485_
m user id: 0
owner id: 6618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23114
component: BBa_K360121
sequence annotation: BBa_K360121
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K360128_sequence/1

BBa_J23114
type: Component
persistent identity: component2091975
display id: component2091975
version: 1
top level: BBa_K360128
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_K360121
type: Component
persistent identity: component2091982
display id: component2091982
version: 1
top level: BBa_K360128
access: public
definition: BBa_K360121

BBa_K360121
type: SequenceAnnotation
persistent identity: annotation2091982
display id: annotation2091982
version: 1
top level: BBa_K360128
location: https://synbiohub.org/public/igem/BBa_K360128/annotation2091982/range2091982/1
component: BBa_K360121

https://synbiohub.org/public/igem/BBa_K360128/annotation2091982/range2091982/1
type: Range
persistent identity: range2091982
display id: range2091982
version: 1
direction: direction/reverse
top level: BBa_K360128
start: 44
end: 887
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2091975
display id: annotation2091975
version: 1
top level: BBa_K360128
location: https://synbiohub.org/public/igem/BBa_K360128/annotation2091975/range2091975/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K360128/annotation2091975/range2091975/1
type: Range
persistent identity: range2091975
display id: range2091975
version: 1
direction: direction/reverse
top level: BBa_K360128
start: 1
end: 35
orientation: inline

BBa_I712004
type: ComponentDefinition
persistent identity: BBa I712004
display id: BBa_I712004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV promoter
creator: Andrej Ondracka
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included.
mutable notes: Cloned into pSB1AC3 vector.
mutable provenance: pcDNA3 by Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712004_sequence/1

BBa_K633001
type: ComponentDefinition
persistent identity: BBa K633001
display id: BBa_K633001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linker + EstA Membrane Protein
creator: Ricardo Camilo Chavez Martinez
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _807_
m user id: 0
owner id: 9146
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We used a linker attached to a protein membrane "estA"
mutable notes: None whatsoever
mutable provenance: where does it come from?
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: estA
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_K633001_sequence/1

estA
type: SequenceAnnotation
persistent identity: annotation2145003
display id: annotation2145003
version: 1
top level: BBa_K633001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K633001/annotation2145003/range2145003/1

https://synbiohub.org/public/igem/BBa_K633001/annotation2145003/range2145003/1
type: Range
persistent identity: range2145003
display id: range2145003
version: 1
direction: direction/forward
top level: BBa_K633001
start: 34
end: 876
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2145004
display id: annotation2145004
version: 1
top level: BBa_K633001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K633001/annotation2145004/range2145004/1

https://synbiohub.org/public/igem/BBa_K633001/annotation2145004/range2145004/1
type: Range
persistent identity: range2145004
display id: range2145004
version: 1
direction: direction/forward
top level: BBa_K633001
start: 1
end: 33
orientation: inline

mRFPr
type: ComponentDefinition
persistent identity: BBa J31008
display id: BBa_J31008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP reverse
creator: Karmella Haynes
created: 2007-02-15T12:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed
mutable notes: RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers: 5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F" 5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"
mutable provenance: RFP reverse was PCR amplified from mRFP (BBaE1010).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mRFP reverse
sequence annotation: Spe mRFP F
sequence annotation: Xba mRFP R
sequence: https://synbiohub.org/public/igem/BBa_J31008_sequence/1

mRFP reverse
type: SequenceAnnotation
persistent identity: annotation1911761
display id: annotation1911761
version: 1
top level: mRFPr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1

https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
type: Range
persistent identity: range1911761
display id: range1911761
version: 1
direction: direction/reverse
top level: mRFPr
start: 1
end: 678
orientation: inline

Spe mRFP F
type: SequenceAnnotation
persistent identity: annotation1911764
display id: annotation1911764
version: 1
top level: mRFPr
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1

https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
type: Range
persistent identity: range1911764
display id: range1911764
version: 1
direction: direction/reverse
top level: mRFPr
start: 659
end: 678
orientation: inline

Xba mRFP R
type: SequenceAnnotation
persistent identity: annotation1911765
display id: annotation1911765
version: 1
top level: mRFPr
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1

https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
type: Range
persistent identity: range1911765
display id: range1911765
version: 1
direction: direction/forward
top level: mRFPr
start: 1
end: 20
orientation: inline

BBa_K1033933
type: ComponentDefinition
persistent identity: BBa K1033933
display id: BBa_K1033933
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asPink, pink chromoprotein
creator: Erik Lundin
created: 2014-04-20T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: asPink
sequence: https://synbiohub.org/public/igem/BBa_K1033933_sequence/1

asPink
type: SequenceAnnotation
persistent identity: annotation2379047
display id: annotation2379047
version: 1
top level: BBa_K1033933
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033933/annotation2379047/range2379047/1

https://synbiohub.org/public/igem/BBa_K1033933/annotation2379047/range2379047/1
type: Range
persistent identity: range2379047
display id: range2379047
version: 1
direction: direction/forward
top level: BBa_K1033933
start: 1
end: 702
orientation: inline

BBa_K879011
type: ComponentDefinition
persistent identity: BBa K879011
display id: BBa_K879011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP
creator: Nien-Tsu Tseng
created: 2012-09-27T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The part contains RFP sequence from E1010 with removal of Kpn I site and stop ending changed to tgatga
mutable notes: .
mutable provenance: E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K879011_sequence/1

BBa_M45116
type: ComponentDefinition
persistent identity: BBa M45116
display id: BBa_M45116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFPuv
creator: Federico C. Rodriguez
created: 2014-04-18T11:00:00Z
modified: 2015-05-08T01:14:08Z
discontinued: false
dominant: false
group u list: _1855_
m user id: 0
owner id: 20936
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Green fluorescent protein (GFP) is a 27 kDa protein of 238 amino acids in length. GFP is intrinsically fluorescent and hence is ideal as a non-invasive marker in living cells. It is used as a cell lineage tracer, reporter of gene expression, a measure of protein-protein interactions, and as an intracellular pH indicator. GFP can function as a protein tag as it tolerates N- and C-terminal fusion to a broad variety of proteins (Chin et al.). Wild-type GFP fluoresces maximally when excited at 395 nm, with a minor peak at 475 nm. Fluorescence emission peaks at 509 nm. It is stable within a range of pH 5.5 to 12. Denaturation occurs at temperatures above 70??C (Chin et al.). Several variants with improved absorption or emission spectra, or increased the fluorescence intensity, have since been developed. Variants also exhibit different thermosensitivities (Chin et al.). As stated before, the maximum excitation spectra of GFPuv is 395nm while its maximum emission spectra is 509nm, which is similar to that of wild-type GFP. Compared to wild-type GFP, GFPuv contains three amino acid substitutions (Phe-99 to Ser, Met-153 to Thr, and Val-163 to Ala [based on the amino acid numbering of wild-type GFP]), none of which alter the chromophore sequence. The main advantage of this variant over wild-type GFP is its large amount of fluorescence when excited by ultra-violet (UV) light at 395nm. GFPuv is 18 times brighter than wild-type GFP under ultra-violet excitation and can easily be detected by the eye without the need for specialized equipment. Furthermore, GFPuv expressed in E. coli is a soluble, fluorescent protein in contrast to wild-type GFP, much of which may be expressed as a nonfluorescent protein in inclusion bodies. Consequently, the GFPuv gene is expressed very efficiently in E. coli (Chin et al.). Reference: Chin et al. Characterization of Green Fluorescent Protein. Retrieved from: http://staff.science.nus.edu.sg/~scilooe/srp_2003/sci_paper/dbs/research%20_paper/ngeow_ka…
mutable notes: The sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be taken into account during the creation of the sequence as a biobrick.
mutable provenance: GFPuv comes was originally isolated from the bioluminescent jellyfish Aequorea Victoria (Chin et al.).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding Sequence
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M45116_sequence/1

Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2372547
display id: annotation2372547
version: 1
top level: BBa_M45116
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M45116/annotation2372547/range2372547/1

https://synbiohub.org/public/igem/BBa_M45116/annotation2372547/range2372547/1
type: Range
persistent identity: range2372547
display id: range2372547
version: 1
direction: direction/forward
top level: BBa_M45116
start: 1
end: 717
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2372548
display id: annotation2372548
version: 1
top level: BBa_M45116
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M45116/annotation2372548/range2372548/1

https://synbiohub.org/public/igem/BBa_M45116/annotation2372548/range2372548/1
type: Range
persistent identity: range2372548
display id: range2372548
version: 1
direction: direction/forward
top level: BBa_M45116
start: 1
end: 3
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2372549
display id: annotation2372549
version: 1
top level: BBa_M45116
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M45116/annotation2372549/range2372549/1

https://synbiohub.org/public/igem/BBa_M45116/annotation2372549/range2372549/1
type: Range
persistent identity: range2372549
display id: range2372549
version: 1
direction: direction/forward
top level: BBa_M45116
start: 715
end: 717
orientation: inline

BBa_K763000
type: ComponentDefinition
persistent identity: BBa K763000
display id: BBa_K763000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac + Gene encoding AmCyan
creator: Pedro Luis Dorado Morales
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1014_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The promoter is activated when: (1) there is no glucose in the medium and (2) there is lactose in the medium. Our construction uses the well-known lactose operon system. A lack of glucose promotes the formation of CAP (or CRP), which binds to specific sites upstream of sugar-metabolization genes and activates its transcription. Moreover, another condition is needed since LacI repressor will bind to the operator region and will block the transcription unless lactose is also in the medium.
mutable notes: No design considerations needed.
mutable provenance: The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K763000_sequence/1

BBa_K175041
type: ComponentDefinition
persistent identity: BBa K175041
display id: BBa_K175041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p(LacI) controlled I-SceI homing endonuclease generator
creator: Tim_Weenink
created: 2009-10-10T11:00:00Z
modified: 2015-05-08T01:10:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _280_
m user id: 0
owner id: 4302
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: p(LacI) controlled I-SceI homing endonuclease generator, inducible with IPTG when LacI is present in the cell
mutable notes: BBa_K142205 is wrong, so this part was assembled again.
mutable provenance: Assembled with BBa_K142202 from iGEM08_ETH_Zurich.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_B0012
component: BBa_K142200
component: BBa_B0010
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_K142200
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K175041_sequence/1

BBa_B0030
type: Component
persistent identity: component2033677
display id: component2033677
version: 1
top level: BBa_K175041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component2033683
display id: component2033683
version: 1
top level: BBa_K175041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K142200
type: Component
persistent identity: component2033680
display id: component2033680
version: 1
top level: BBa_K175041
access: public
definition: https://synbiohub.org/public/igem/BBa_K142200/1

BBa_B0010
type: Component
persistent identity: component2033681
display id: component2033681
version: 1
top level: BBa_K175041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component2033669
display id: component2033669
version: 1
top level: BBa_K175041
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2033669
display id: annotation2033669
version: 1
top level: BBa_K175041
location: https://synbiohub.org/public/igem/BBa_K175041/annotation2033669/range2033669/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K175041/annotation2033669/range2033669/1
type: Range
persistent identity: range2033669
display id: range2033669
version: 1
direction: direction/forward
top level: BBa_K175041
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033681
display id: annotation2033681
version: 1
top level: BBa_K175041
location: https://synbiohub.org/public/igem/BBa_K175041/annotation2033681/range2033681/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K175041/annotation2033681/range2033681/1
type: Range
persistent identity: range2033681
display id: range2033681
version: 1
direction: direction/forward
top level: BBa_K175041
start: 986
end: 1065
orientation: inline

BBa_K142200
type: SequenceAnnotation
persistent identity: annotation2033680
display id: annotation2033680
version: 1
top level: BBa_K175041
location: https://synbiohub.org/public/igem/BBa_K175041/annotation2033680/range2033680/1
component: BBa_K142200

https://synbiohub.org/public/igem/BBa_K175041/annotation2033680/range2033680/1
type: Range
persistent identity: range2033680
display id: range2033680
version: 1
direction: direction/reverse
top level: BBa_K175041
start: 230
end: 977
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2033677
display id: annotation2033677
version: 1
top level: BBa_K175041
location: https://synbiohub.org/public/igem/BBa_K175041/annotation2033677/range2033677/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K175041/annotation2033677/range2033677/1
type: Range
persistent identity: range2033677
display id: range2033677
version: 1
direction: direction/forward
top level: BBa_K175041
start: 209
end: 223
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2033683
display id: annotation2033683
version: 1
top level: BBa_K175041
location: https://synbiohub.org/public/igem/BBa_K175041/annotation2033683/range2033683/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K175041/annotation2033683/range2033683/1
type: Range
persistent identity: range2033683
display id: range2033683
version: 1
direction: direction/forward
top level: BBa_K175041
start: 1074
end: 1114
orientation: inline

Δ12d
type: ComponentDefinition
persistent identity: BBa K925000
display id: BBa_K925000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Delta-12 desaturase
creator: Veronica Rezelj
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1190_
m user id: 0
owner id: 12788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a delta-12 desaturase derived from Synechocystis sp PCC 6803. The enzyme is able to introduce a double bond at the Δ-12 site in the hydrocarbon chain of oleic acid (18:1 , Δ9), for its conversion into linoleic acid (18:2 Δ9,12), a polyunsaturated fatty acid (PUFA). Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part, the bacterial membrane composition is changed, and linoleic acid is observed.
mutable notes: .
mutable provenance: This desaturase comes from Synechocystis sp PCC 6803 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: d12des
sequence: https://synbiohub.org/public/igem/BBa_K925000_sequence/1

d12des
type: SequenceAnnotation
persistent identity: annotation2196200
display id: annotation2196200
version: 1
top level: Δ12d
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K925000/annotation2196200/range2196200/1

https://synbiohub.org/public/igem/BBa_K925000/annotation2196200/range2196200/1
type: Range
persistent identity: range2196200
display id: range2196200
version: 1
direction: direction/forward
top level: Δ12d
start: 1
end: 1056
orientation: inline

BBa_K331023
type: ComponentDefinition
persistent identity: BBa K331023
display id: BBa_K331023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS with C-terminal Oligo Arginine - EYFP Fusion
creator: Adam Smith
created: 2010-09-28T11:00:00Z
modified: 2015-05-08T01:12:04Z
discontinued: false
dominant: false
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a oligo arginine sequence fused to the C-terminus of an enhanced yellow fluorescent protein. This will be a component of the proof-of-concept part K331019 which will show localization of proteins into the interior of a microcompartment formed by the assemly of lumazine synthase proteins.
mutable notes: We chose not to include a transcriptional promoter to this part because we wanted the freedom to put it under the control of varied promoters. We also chose not to include a transcriptional terminator to this part because we planned to use it in an operon, where we would be expressing multiple proteins from a single transcript.
mutable provenance: This sequence was synthesized by Gene Art
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K249006
sequence annotation: BBa_B0034
sequence annotation: BBa_K249006
sequence: https://synbiohub.org/public/igem/BBa_K331023_sequence/1

BBa_B0034
type: Component
persistent identity: component2081110
display id: component2081110
version: 1
top level: BBa_K331023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K249006
type: Component
persistent identity: component2081111
display id: component2081111
version: 1
top level: BBa_K331023
access: public
definition: BBa_K249006

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2081110
display id: annotation2081110
version: 1
top level: BBa_K331023
location: https://synbiohub.org/public/igem/BBa_K331023/annotation2081110/range2081110/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331023/annotation2081110/range2081110/1
type: Range
persistent identity: range2081110
display id: range2081110
version: 1
direction: direction/reverse
top level: BBa_K331023
start: 1
end: 12
orientation: inline

BBa_K249006
type: SequenceAnnotation
persistent identity: annotation2081111
display id: annotation2081111
version: 1
top level: BBa_K331023
location: https://synbiohub.org/public/igem/BBa_K331023/annotation2081111/range2081111/1
component: BBa_K249006

https://synbiohub.org/public/igem/BBa_K331023/annotation2081111/range2081111/1
type: Range
persistent identity: range2081111
display id: range2081111
version: 1
direction: direction/reverse
top level: BBa_K331023
start: 19
end: 774
orientation: inline

BBa_P0455
type: ComponentDefinition
persistent identity: BBa P0455
display id: BBa_P0455
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.Lambda cI LVA+.Term
creator: Reshma Shetty
created: 2005-06-29T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_B0012
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_P0455_sequence/1

BBa_B0011
type: Component
persistent identity: component1558091
display id: component1558091
version: 1
top level: BBa_P0455
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component1558083
display id: component1558083
version: 1
top level: BBa_P0455
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0051
type: Component
persistent identity: component1558073
display id: component1558073
version: 1
top level: BBa_P0455
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component1558063
display id: component1558063
version: 1
top level: BBa_P0455
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1558073
display id: annotation1558073
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558073/range1558073/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0455/annotation1558073/range1558073/1
type: Range
persistent identity: range1558073
display id: range1558073
version: 1
direction: direction/forward
top level: BBa_P0455
start: 19
end: 768
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1558083
display id: annotation1558083
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558083/range1558083/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0455/annotation1558083/range1558083/1
type: Range
persistent identity: range1558083
display id: range1558083
version: 1
direction: direction/forward
top level: BBa_P0455
start: 802
end: 842
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1558063
display id: annotation1558063
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558063/range1558063/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0455/annotation1558063/range1558063/1
type: Range
persistent identity: range1558063
display id: range1558063
version: 1
direction: direction/forward
top level: BBa_P0455
start: 1
end: 12
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1558091
display id: annotation1558091
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558091/range1558091/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_P0455/annotation1558091/range1558091/1
type: Range
persistent identity: range1558091
display id: range1558091
version: 1
direction: direction/forward
top level: BBa_P0455
start: 851
end: 896
orientation: inline

SheDnaK
type: ComponentDefinition
persistent identity: BBa K538002
display id: BBa_K538002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SheDnaK (Shewanella sp. Ac10)
creator: Bas Stringer (Sequence by Paul van Dieken)
created: 2011-07-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A > G (Recode for PvuII site)
sequence annotation: Start Codon
sequence annotation: A > G (Recode for EcoRI site)
sequence annotation: T > G (Recode for XbaI site)
sequence annotation: T > G (Recode for NheI site)
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K538002_sequence/1

A > G (Recode for PvuII site)
type: SequenceAnnotation
persistent identity: annotation2127787
display id: annotation2127787
version: 1
top level: SheDnaK
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K538002/annotation2127787/range2127787/1

https://synbiohub.org/public/igem/BBa_K538002/annotation2127787/range2127787/1
type: Range
persistent identity: range2127787
display id: range2127787
version: 1
direction: direction/forward
top level: SheDnaK
start: 345
end: 345
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2124233
display id: annotation2124233
version: 1
top level: SheDnaK
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K538002/annotation2124233/range2124233/1

https://synbiohub.org/public/igem/BBa_K538002/annotation2124233/range2124233/1
type: Range
persistent identity: range2124233
display id: range2124233
version: 1
direction: direction/forward
top level: SheDnaK
start: 1
end: 3
orientation: inline

A > G (Recode for EcoRI site)
type: SequenceAnnotation
persistent identity: annotation2127789
display id: annotation2127789
version: 1
top level: SheDnaK
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K538002/annotation2127789/range2127789/1

https://synbiohub.org/public/igem/BBa_K538002/annotation2127789/range2127789/1
type: Range
persistent identity: range2127789
display id: range2127789
version: 1
direction: direction/forward
top level: SheDnaK
start: 726
end: 726
orientation: inline

T > G (Recode for XbaI site)
type: SequenceAnnotation
persistent identity: annotation2127790
display id: annotation2127790
version: 1
top level: SheDnaK
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K538002/annotation2127790/range2127790/1

https://synbiohub.org/public/igem/BBa_K538002/annotation2127790/range2127790/1
type: Range
persistent identity: range2127790
display id: range2127790
version: 1
direction: direction/forward
top level: SheDnaK
start: 945
end: 945
orientation: inline

T > G (Recode for NheI site)
type: SequenceAnnotation
persistent identity: annotation2127792
display id: annotation2127792
version: 1
top level: SheDnaK
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K538002/annotation2127792/range2127792/1

https://synbiohub.org/public/igem/BBa_K538002/annotation2127792/range2127792/1
type: Range
persistent identity: range2127792
display id: range2127792
version: 1
direction: direction/forward
top level: SheDnaK
start: 1671
end: 1671
orientation: inline

A > G (Recode for PvuII site)
type: SequenceAnnotation
persistent identity: annotation2127791
display id: annotation2127791
version: 1
top level: SheDnaK
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K538002/annotation2127791/range2127791/1

https://synbiohub.org/public/igem/BBa_K538002/annotation2127791/range2127791/1
type: Range
persistent identity: range2127791
display id: range2127791
version: 1
direction: direction/forward
top level: SheDnaK
start: 1281
end: 1281
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2124234
display id: annotation2124234
version: 1
top level: SheDnaK
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K538002/annotation2124234/range2124234/1

https://synbiohub.org/public/igem/BBa_K538002/annotation2124234/range2124234/1
type: Range
persistent identity: range2124234
display id: range2124234
version: 1
direction: direction/forward
top level: SheDnaK
start: 1915
end: 1920
orientation: inline

BBa_K777109
type: ComponentDefinition
persistent identity: BBa K777109
display id: BBa_K777109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliC
creator: Flash Coli
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: * The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. Coli''.
mutable notes: * The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR. * ''flhDC'' was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: ACTGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTG ** Rev: TCCTGCAGCGGCCGCTACTAGTTACTGCCCGGGATGGCGGTTGACATAAGCcGCAGGCAAAGCTGCCAACAG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SpeI site removed
sequence annotation: TAA stop codon
sequence annotation: FliC coding region
sequence annotation: PstI site removed
sequence annotation: ATG start codon
sequence: https://synbiohub.org/public/igem/BBa_K777109_sequence/1

SpeI site removed
type: SequenceAnnotation
persistent identity: annotation2184554
display id: annotation2184554
version: 1
top level: BBa_K777109
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184554/range2184554/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184554/range2184554/1
type: Range
persistent identity: range2184554
display id: range2184554
version: 1
direction: direction/forward
top level: BBa_K777109
start: 552
end: 552
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2184550
display id: annotation2184550
version: 1
top level: BBa_K777109
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184550/range2184550/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184550/range2184550/1
type: Range
persistent identity: range2184550
display id: range2184550
version: 1
direction: direction/forward
top level: BBa_K777109
start: 1495
end: 1497
orientation: inline

FliC coding region
type: SequenceAnnotation
persistent identity: annotation2184548
display id: annotation2184548
version: 1
top level: BBa_K777109
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184548/range2184548/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184548/range2184548/1
type: Range
persistent identity: range2184548
display id: range2184548
version: 1
direction: direction/forward
top level: BBa_K777109
start: 1
end: 1497
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2184560
display id: annotation2184560
version: 1
top level: BBa_K777109
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184560/range2184560/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184560/range2184560/1
type: Range
persistent identity: range2184560
display id: range2184560
version: 1
direction: direction/forward
top level: BBa_K777109
start: 1488
end: 1488
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2184559
display id: annotation2184559
version: 1
top level: BBa_K777109
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184559/range2184559/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184559/range2184559/1
type: Range
persistent identity: range2184559
display id: range2184559
version: 1
direction: direction/forward
top level: BBa_K777109
start: 921
end: 921
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2184558
display id: annotation2184558
version: 1
top level: BBa_K777109
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184558/range2184558/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184558/range2184558/1
type: Range
persistent identity: range2184558
display id: range2184558
version: 1
direction: direction/forward
top level: BBa_K777109
start: 876
end: 876
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2184549
display id: annotation2184549
version: 1
top level: BBa_K777109
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184549/range2184549/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184549/range2184549/1
type: Range
persistent identity: range2184549
display id: range2184549
version: 1
direction: direction/forward
top level: BBa_K777109
start: 1
end: 3
orientation: inline

BBa_K125502
type: ComponentDefinition
persistent identity: BBa K125502
display id: BBa_K125502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1993)
creator: Norman Wang
created: 2008-08-09T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: phaB
mutable notes: PHB biosynthesis pathway
mutable provenance: Synechocystis sp. PCC6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: inserted second stop
sequence annotation: start
sequence annotation: original stop codon
sequence: https://synbiohub.org/public/igem/BBa_K125502_sequence/1

inserted second stop
type: SequenceAnnotation
persistent identity: annotation1971710
display id: annotation1971710
version: 1
top level: BBa_K125502
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971710/range1971710/1

https://synbiohub.org/public/igem/BBa_K125502/annotation1971710/range1971710/1
type: Range
persistent identity: range1971710
display id: range1971710
version: 1
direction: direction/forward
top level: BBa_K125502
start: 724
end: 726
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1971708
display id: annotation1971708
version: 1
top level: BBa_K125502
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971708/range1971708/1

https://synbiohub.org/public/igem/BBa_K125502/annotation1971708/range1971708/1
type: Range
persistent identity: range1971708
display id: range1971708
version: 1
direction: direction/forward
top level: BBa_K125502
start: 1
end: 3
orientation: inline

original stop codon
type: SequenceAnnotation
persistent identity: annotation1971709
display id: annotation1971709
version: 1
top level: BBa_K125502
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971709/range1971709/1

https://synbiohub.org/public/igem/BBa_K125502/annotation1971709/range1971709/1
type: Range
persistent identity: range1971709
display id: range1971709
version: 1
direction: direction/forward
top level: BBa_K125502
start: 721
end: 723
orientation: inline

BBa_K249008
type: ComponentDefinition
persistent identity: BBa K249008
display id: BBa_K249008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP with N-terminal Arganine Tag
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This Yellow Fluorescent protein has been fused with 10 N-terminal Arginines to target it into the Lumazine-based microcompartment. Has a double terminator at the end
mutable notes: Standard
mutable provenance: BioBrick YFP with synthesized N-termianl Arg Tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K249008_sequence/1

BBa_I739001
type: ComponentDefinition
persistent identity: BBa I739001
display id: BBa_I739001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)
creator: Christian Kemmer, Stefan Luzi
created: 2007-09-26T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2208
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Biobrick encodes tetR (BBa_C0040) under control of the constitutive promoter (BBa_J23100) followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015
mutable notes: expression cassette for tetR
mutable provenance: synthetic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_J23105
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J23105
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I739001_sequence/1

BBa_C0040
type: Component
persistent identity: component2253166
display id: component2253166
version: 1
top level: BBa_I739001
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J23105
type: Component
persistent identity: component2253160
display id: component2253160
version: 1
top level: BBa_I739001
access: public
definition: https://synbiohub.org/public/igem/BBa_J23105/1

BBa_B0015
type: Component
persistent identity: component2253173
display id: component2253173
version: 1
top level: BBa_I739001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2253162
display id: component2253162
version: 1
top level: BBa_I739001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2253162
display id: annotation2253162
version: 1
top level: BBa_I739001
location: https://synbiohub.org/public/igem/BBa_I739001/annotation2253162/range2253162/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I739001/annotation2253162/range2253162/1
type: Range
persistent identity: range2253162
display id: range2253162
version: 1
direction: direction/reverse
top level: BBa_I739001
start: 44
end: 55
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2253160
display id: annotation2253160
version: 1
top level: BBa_I739001
location: https://synbiohub.org/public/igem/BBa_I739001/annotation2253160/range2253160/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_I739001/annotation2253160/range2253160/1
type: Range
persistent identity: range2253160
display id: range2253160
version: 1
direction: direction/reverse
top level: BBa_I739001
start: 1
end: 35
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2253166
display id: annotation2253166
version: 1
top level: BBa_I739001
location: https://synbiohub.org/public/igem/BBa_I739001/annotation2253166/range2253166/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I739001/annotation2253166/range2253166/1
type: Range
persistent identity: range2253166
display id: range2253166
version: 1
direction: direction/reverse
top level: BBa_I739001
start: 62
end: 746
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2253173
display id: annotation2253173
version: 1
top level: BBa_I739001
location: https://synbiohub.org/public/igem/BBa_I739001/annotation2253173/range2253173/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I739001/annotation2253173/range2253173/1
type: Range
persistent identity: range2253173
display id: range2253173
version: 1
direction: direction/reverse
top level: BBa_I739001
start: 755
end: 883
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR 2
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

Fok_a
type: ComponentDefinition
persistent identity: BBa K243000
display id: BBa_K243000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein domain (active) of the restriction endonuclease FokI
creator: Freiburg Bioware09
created: 2009-10-07T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is used as the active domain of our universal restriction endonuclease. It cut double stranded DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001).
mutable notes: Modifications of the vector (catalytic active heterodimer) -heterodimeric aminio acids * switch Glutamate 490/310-312 to Lysin (GAA->AAA) * switch isoleucin 538/454-456 to Lysin (ATC->AAA)
mutable provenance: extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Fok_a
sequence: https://synbiohub.org/public/igem/BBa_K243000_sequence/1

Fok_a
type: SequenceAnnotation
persistent identity: annotation2041869
display id: annotation2041869
version: 1
top level: Fok_a
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K243000/annotation2041869/range2041869/1

https://synbiohub.org/public/igem/BBa_K243000/annotation2041869/range2041869/1
type: Range
persistent identity: range2041869
display id: range2041869
version: 1
direction: direction/forward
top level: Fok_a
start: 1
end: 579
orientation: inline

BBa_K249003
type: ComponentDefinition
persistent identity: BBa K249003
display id: BBa_K249003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lumazine Synthase
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2016-10-08T12:35:49Z
discontinued: false
dominant: true
experience: experience/Fails
group u list: _342_
m user id: 31248
owner id: 3171
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the Composite Part which creates the enzyme Lumazine Synthase. Lumazine SYnthase generates the product Lumazine which aggregates together to form a microcompartment, or synthetic organelle. The microcompartment has large negatively charged pores through which proteins containing positely charged Arginine Tags may be targetted.
mutable notes: point mutations to EcoRI and PstI sites?
mutable provenance: A science paper?
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0012
component: BBa_K249002
component: BBa_R0010
component: BBa_B0010
component: BBa_B0030
sequence annotation: BBa_K249002
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K249003_sequence/1

BBa_B0012
type: Component
persistent identity: component2060532
display id: component2060532
version: 1
top level: BBa_K249003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K249002
type: Component
persistent identity: component2060529
display id: component2060529
version: 1
top level: BBa_K249003
access: public
definition: BBa_K249002

BBa_R0010
type: Component
persistent identity: component2060519
display id: component2060519
version: 1
top level: BBa_K249003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component2060530
display id: component2060530
version: 1
top level: BBa_K249003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component2060527
display id: component2060527
version: 1
top level: BBa_K249003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K249002
type: SequenceAnnotation
persistent identity: annotation2060529
display id: annotation2060529
version: 1
top level: BBa_K249003
location: https://synbiohub.org/public/igem/BBa_K249003/annotation2060529/range2060529/1
component: BBa_K249002

https://synbiohub.org/public/igem/BBa_K249003/annotation2060529/range2060529/1
type: Range
persistent identity: range2060529
display id: range2060529
version: 1
direction: direction/reverse
top level: BBa_K249003
start: 230
end: 694
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2060530
display id: annotation2060530
version: 1
top level: BBa_K249003
location: https://synbiohub.org/public/igem/BBa_K249003/annotation2060530/range2060530/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K249003/annotation2060530/range2060530/1
type: Range
persistent identity: range2060530
display id: range2060530
version: 1
direction: direction/forward
top level: BBa_K249003
start: 703
end: 782
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2060532
display id: annotation2060532
version: 1
top level: BBa_K249003
location: https://synbiohub.org/public/igem/BBa_K249003/annotation2060532/range2060532/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K249003/annotation2060532/range2060532/1
type: Range
persistent identity: range2060532
display id: range2060532
version: 1
direction: direction/forward
top level: BBa_K249003
start: 791
end: 831
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2060519
display id: annotation2060519
version: 1
top level: BBa_K249003
location: https://synbiohub.org/public/igem/BBa_K249003/annotation2060519/range2060519/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K249003/annotation2060519/range2060519/1
type: Range
persistent identity: range2060519
display id: range2060519
version: 1
direction: direction/forward
top level: BBa_K249003
start: 1
end: 200
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2060527
display id: annotation2060527
version: 1
top level: BBa_K249003
location: https://synbiohub.org/public/igem/BBa_K249003/annotation2060527/range2060527/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K249003/annotation2060527/range2060527/1
type: Range
persistent identity: range2060527
display id: range2060527
version: 1
direction: direction/forward
top level: BBa_K249003
start: 209
end: 223
orientation: inline

BBa_K1051000
type: ComponentDefinition
persistent identity: BBa K1051000
display id: BBa_K1051000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stop codon free RFP in RFC[23] standard
creator: Xiang LI
created: 2013-07-01T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15912
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Red fluorescent protein with assembly standard 23 is engineered mutant of red fluorescent protein from Discosoma striata (coral).
mutable notes: You have to remove the termination codon before you use it
mutable provenance: engineered mutant of red fluorescent protein from Discosoma striata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1051000_sequence/1

BBa_K300002
type: ComponentDefinition
persistent identity: BBa K300002
display id: BBa_K300002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phasin (PhaP) - head domain
creator: Giacomo Zambianchi, Alessandro Ranieri, Manuel Lupotto, Paolo Magni
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: It is identical to BBa_K208001 , but it lacks the stop codon in order to support protein fusions. It has been designed as a head domain: The Prefix sequence is 5'-GAATTCGCGGCCGCTTCTAG-3' (RFC10 Prefix) The Suffix sequence is 5'-ACTAGTAGCGGCCGCTGCAG-3' (RFC23 Suffix) For these reasons, a tail domain or an internal domain (compatible with RFC23) are ready to be assembled downstream to create protein fusions. CONSTRUCTION METHOD: BBa_K208001 (provided by iGEM HQ in pSB3K3 in RFC23 standard) was PCR-amplified/mutagenized with primers: phaP10F 5'-GCTTCTAGATGATCCTCACCCCGGAACA-3' phaPSR: 5'-GCTACTAGTGGCAGCCGTCGTCTTCTTTG-3' in order to delete the stop codon. The PCR product was ran on a 1% agarose gel, gel-extracted, cut with XbaI-SpeI and ligated with pSB1AK3 (previously cut with XbaI-SpeI and dephosphorylated). Positive clones were found through digestion screening/sequencing.
mutable provenance: Ralstonia eutropha genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K300002_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2081800
display id: annotation2081800
version: 1
top level: BBa_K300002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K300002/annotation2081800/range2081800/1

https://synbiohub.org/public/igem/BBa_K300002/annotation2081800/range2081800/1
type: Range
persistent identity: range2081800
display id: range2081800
version: 1
direction: direction/forward
top level: BBa_K300002
start: 1
end: 3
orientation: inline

BBa_K873003
type: ComponentDefinition
persistent identity: BBa K873003
display id: BBa_K873003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: conjugative transfer of RFG
creator: yue hu
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1134_
m user id: 0
owner id: 11547
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We just combine part J01003 with part I13521, J01003 is coden oriT and can transfer the sequence that followed.
mutable notes: This part can just be moblized by other plasmids which contain tra gene.
mutable provenance: Both the two parts we used were from registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
component: BBa_J01003
component: BBa_I13521
sequence annotation: BBa_J01003
sequence annotation: BBa_I13521
sequence: https://synbiohub.org/public/igem/BBa_K873003_sequence/1

BBa_J01003
type: Component
persistent identity: component2185359
display id: component2185359
version: 1
top level: BBa_K873003
access: public
definition: https://synbiohub.org/public/igem/BBa_J01003/1

BBa_I13521
type: Component
persistent identity: component2185376
display id: component2185376
version: 1
top level: BBa_K873003
access: public
definition: https://synbiohub.org/public/igem/BBa_I13521/1

BBa_J01003
type: SequenceAnnotation
persistent identity: annotation2185359
display id: annotation2185359
version: 1
top level: BBa_K873003
location: https://synbiohub.org/public/igem/BBa_K873003/annotation2185359/range2185359/1
component: BBa_J01003

https://synbiohub.org/public/igem/BBa_K873003/annotation2185359/range2185359/1
type: Range
persistent identity: range2185359
display id: range2185359
version: 1
direction: direction/reverse
top level: BBa_K873003
start: 1
end: 371
orientation: inline

BBa_I13521
type: SequenceAnnotation
persistent identity: annotation2185376
display id: annotation2185376
version: 1
top level: BBa_K873003
location: https://synbiohub.org/public/igem/BBa_K873003/annotation2185376/range2185376/1
component: BBa_I13521

https://synbiohub.org/public/igem/BBa_K873003/annotation2185376/range2185376/1
type: Range
persistent identity: range2185376
display id: range2185376
version: 1
direction: direction/reverse
top level: BBa_K873003
start: 380
end: 1302
orientation: inline

BBa_K763004
type: ComponentDefinition
persistent identity: BBa K763004
display id: BBa_K763004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLexA + Gene encoding ZsGreen1
creator: Pedro Luis Dorado Morales
created: 2012-09-06T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1014_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: When E.coli cells are irradiated with UV light (240 nm), bacteria start synthesizing the fluorescent protein. Why? Eubacteria respond to DNA damage with a set of mechanisms called SOS response. The induction of this system involves more than forty independent SOS genes, most of which encode proteins engaged in protection, repairing, replication, mutagenesis and metabolism of DNA. How does this system work? Under normal conditions, LexA protein represses the transcription of many DNA repairing proteins by binding to some sequences called SOS ???boxes??? in their operators. But, when a damage in the DNA occurs, (different types of genotoxic stress can make this system start), the accumulation RecA-ssDNA-ATP complexes activate LexA for autocleavage.
mutable notes: No design considerations needed.
mutable provenance: The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K763004_sequence/1

BBa_K518005
type: ComponentDefinition
persistent identity: BBa K518005
display id: BBa_K518005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ
creator: Masato Ohgishi
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility.
mutable notes: None.
mutable provenance: Cloned from Escherichia coli JM109 strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K518005_sequence/1

BBa_K362004
type: ComponentDefinition
persistent identity: BBa K362004
display id: BBa_K362004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sodA promoter is controlled by some transcription factors including SoxS.
creator: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama
created: 2010-09-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _480_
m user id: 0
owner id: 6267
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sensitibity to oxiditative stress
mutable notes: sensitibity to oxiditative stress
mutable provenance: sensitibity to oxiditative stress
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K362004_sequence/1

BBa_K098997
type: ComponentDefinition
persistent identity: BBa K098997
display id: BBa_K098997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cIts coding region
creator: Harvard iGEM 08
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This it the temperature sensitive cI from the PGW7 plasmid. It has the Elowitz RBS in front (BBa_B0034). It produces a temperature sensitive cI repressor that loses binding efficiency with the cI promoter between 35 and 42 degrees C.
mutable notes: Obtained by PCR. RBS also added by PCR.
mutable provenance: It comes from PGW7 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cIts
sequence: https://synbiohub.org/public/igem/BBa_K098997_sequence/1

cIts
type: SequenceAnnotation
persistent identity: annotation1988780
display id: annotation1988780
version: 1
top level: BBa_K098997
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K098997/annotation1988780/range1988780/1

https://synbiohub.org/public/igem/BBa_K098997/annotation1988780/range1988780/1
type: Range
persistent identity: range1988780
display id: range1988780
version: 1
direction: direction/forward
top level: BBa_K098997
start: 1
end: 714
orientation: inline

BBa_K274392
type: ComponentDefinition
persistent identity: BBa K274392
display id: BBa_K274392
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> PhiR73 pag - PP promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746352
component: BBa_B0012
component: BBa_I746362
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746362
sequence annotation: BBa_I746352
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K274392_sequence/1

BBa_I746352
type: Component
persistent identity: component2055392
display id: component2055392
version: 1
top level: BBa_K274392
access: public
definition: https://synbiohub.org/public/igem/BBa_I746352/1

BBa_B0012
type: Component
persistent identity: component2055395
display id: component2055395
version: 1
top level: BBa_K274392
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746362
type: Component
persistent identity: component2055400
display id: component2055400
version: 1
top level: BBa_K274392
access: public
definition: https://synbiohub.org/public/igem/BBa_I746362/1

BBa_B0010
type: Component
persistent identity: component2055393
display id: component2055393
version: 1
top level: BBa_K274392
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055395
display id: annotation2055395
version: 1
top level: BBa_K274392
location: https://synbiohub.org/public/igem/BBa_K274392/annotation2055395/range2055395/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274392/annotation2055395/range2055395/1
type: Range
persistent identity: range2055395
display id: range2055395
version: 1
direction: direction/forward
top level: BBa_K274392
start: 361
end: 401
orientation: inline

BBa_I746362
type: SequenceAnnotation
persistent identity: annotation2055400
display id: annotation2055400
version: 1
top level: BBa_K274392
location: https://synbiohub.org/public/igem/BBa_K274392/annotation2055400/range2055400/1
component: BBa_I746362

https://synbiohub.org/public/igem/BBa_K274392/annotation2055400/range2055400/1
type: Range
persistent identity: range2055400
display id: range2055400
version: 1
direction: direction/reverse
top level: BBa_K274392
start: 410
end: 501
orientation: inline

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2055392
display id: annotation2055392
version: 1
top level: BBa_K274392
location: https://synbiohub.org/public/igem/BBa_K274392/annotation2055392/range2055392/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K274392/annotation2055392/range2055392/1
type: Range
persistent identity: range2055392
display id: range2055392
version: 1
direction: direction/reverse
top level: BBa_K274392
start: 1
end: 264
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055393
display id: annotation2055393
version: 1
top level: BBa_K274392
location: https://synbiohub.org/public/igem/BBa_K274392/annotation2055393/range2055393/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274392/annotation2055393/range2055393/1
type: Range
persistent identity: range2055393
display id: range2055393
version: 1
direction: direction/forward
top level: BBa_K274392
start: 273
end: 352
orientation: inline

BBa_K1088020
type: ComponentDefinition
persistent identity: BBa K1088020
display id: BBa_K1088020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI:LVA device (constitutive promoter, RBS and terminator)
creator: Patrick Rosendahl Andreassen
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The trancsriptional repressor lacI with LVA tag for faster degradation (BBa_C0012)under the constitutivly active promoter (BBa_J23106), with a RBS (BBa_B0030) and with a terminator (BBa_B1002). This device can be used to overexpress lacI with the purpose of repressing transcription from high-copy plasmids containting the lac promoter (BBa_R0010). The repression can be relieved by allosteric binding of IPTG to lacI, thereby promoting transcription from the lac promoter.
mutable notes: Used a strong terminator so that the composite part can be used in front of the lac promoter without unwanted transcription of the regulated gene.
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0030
component: BBa_K1088023
component: BBa_J23106
component: BBa_K1088022
component: BBa_B1002
sequence annotation: BBa_B1002
sequence annotation: BBa_K1088022
sequence annotation: BBa_K1088023
sequence annotation: BBa_J23106
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K1088020_sequence/1

BBa_B0030
type: Component
persistent identity: component2351522
display id: component2351522
version: 1
top level: BBa_K1088020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K1088023
type: Component
persistent identity: component2351527
display id: component2351527
version: 1
top level: BBa_K1088020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1088023/1

BBa_J23106
type: Component
persistent identity: component2351520
display id: component2351520
version: 1
top level: BBa_K1088020
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_K1088022
type: Component
persistent identity: component2351524
display id: component2351524
version: 1
top level: BBa_K1088020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1088022/1

BBa_B1002
type: Component
persistent identity: component2351532
display id: component2351532
version: 1
top level: BBa_K1088020
access: public
definition: https://synbiohub.org/public/igem/BBa_B1002/1

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2351532
display id: annotation2351532
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351532/range2351532/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351532/range2351532/1
type: Range
persistent identity: range2351532
display id: range2351532
version: 1
direction: direction/reverse
top level: BBa_K1088020
start: 1185
end: 1218
orientation: inline

BBa_K1088022
type: SequenceAnnotation
persistent identity: annotation2351524
display id: annotation2351524
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351524/range2351524/1
component: BBa_K1088022

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351524/range2351524/1
type: Range
persistent identity: range2351524
display id: range2351524
version: 1
direction: direction/reverse
top level: BBa_K1088020
start: 51
end: 56
orientation: inline

BBa_K1088023
type: SequenceAnnotation
persistent identity: annotation2351527
display id: annotation2351527
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351527/range2351527/1
component: BBa_K1088023

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351527/range2351527/1
type: Range
persistent identity: range2351527
display id: range2351527
version: 1
direction: direction/reverse
top level: BBa_K1088020
start: 57
end: 1184
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2351520
display id: annotation2351520
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351520/range2351520/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351520/range2351520/1
type: Range
persistent identity: range2351520
display id: range2351520
version: 1
direction: direction/reverse
top level: BBa_K1088020
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2351522
display id: annotation2351522
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351522/range2351522/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351522/range2351522/1
type: Range
persistent identity: range2351522
display id: range2351522
version: 1
direction: direction/forward
top level: BBa_K1088020
start: 36
end: 50
orientation: inline

BBa_K750107
type: ComponentDefinition
persistent identity: BBa K750107
display id: BBa_K750107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBADcIT-0.07:cI protein expression system activated by arabinose(RBS strength:0.07)
creator: Sifan Wang,Yizhen Yan,Shuqin Hu,Yunxin Long,Zhao Ma
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: editing
mutable notes: editing
mutable provenance: editing
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0015
component: BBa_B0031
component: BBa_K206000
component: BBa_C0051
sequence annotation: BBa_B0031
sequence annotation: BBa_K206000
sequence annotation: BBa_B0015
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K750107_sequence/1

BBa_B0015
type: Component
persistent identity: component2259593
display id: component2259593
version: 1
top level: BBa_K750107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0031
type: Component
persistent identity: component2259582
display id: component2259582
version: 1
top level: BBa_K750107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_K206000
type: Component
persistent identity: component2259580
display id: component2259580
version: 1
top level: BBa_K750107
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_C0051
type: Component
persistent identity: component2259586
display id: component2259586
version: 1
top level: BBa_K750107
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2259582
display id: annotation2259582
version: 1
top level: BBa_K750107
location: https://synbiohub.org/public/igem/BBa_K750107/annotation2259582/range2259582/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K750107/annotation2259582/range2259582/1
type: Range
persistent identity: range2259582
display id: range2259582
version: 1
direction: direction/reverse
top level: BBa_K750107
start: 139
end: 152
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2259580
display id: annotation2259580
version: 1
top level: BBa_K750107
location: https://synbiohub.org/public/igem/BBa_K750107/annotation2259580/range2259580/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K750107/annotation2259580/range2259580/1
type: Range
persistent identity: range2259580
display id: range2259580
version: 1
direction: direction/reverse
top level: BBa_K750107
start: 1
end: 130
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259593
display id: annotation2259593
version: 1
top level: BBa_K750107
location: https://synbiohub.org/public/igem/BBa_K750107/annotation2259593/range2259593/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K750107/annotation2259593/range2259593/1
type: Range
persistent identity: range2259593
display id: range2259593
version: 1
direction: direction/reverse
top level: BBa_K750107
start: 942
end: 1070
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2259586
display id: annotation2259586
version: 1
top level: BBa_K750107
location: https://synbiohub.org/public/igem/BBa_K750107/annotation2259586/range2259586/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K750107/annotation2259586/range2259586/1
type: Range
persistent identity: range2259586
display id: range2259586
version: 1
direction: direction/reverse
top level: BBa_K750107
start: 159
end: 933
orientation: inline

Rubber Fuc
type: ComponentDefinition
persistent identity: BBa K1088024
display id: BBa_K1088024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HRT2 prenyltransferase from Hevea Brasilianis (ara promoter without araC: arabinose inducible)
creator: Andreas Kj??r
created: 2013-09-17T11:00:00Z
modified: 2015-05-08T01:09:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The gene is expressed from the arabinose promoter (BBa_I13453), which is induceable with arabinose under glucose scarce conditions. Note that the regulater insn't a part of this part and thus no/very little transcription is expected.
mutable notes: There is a 3xFLAG-tag after the HRT2 stop codon, and thus the 3xFLAG-tag isn't translated.
mutable provenance: Source is specified in components
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1088003
component: BBa_K1088021
component: BBa_I13453
component: BBa_K1088025
component: BBa_J15001
sequence annotation: BBa_I13453
sequence annotation: BBa_K1088003
sequence annotation: BBa_K1088025
sequence annotation: BBa_K1088021
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_K1088024_sequence/1

BBa_K1088003
type: Component
persistent identity: component2373591
display id: component2373591
version: 1
top level: Rubber Fuc
access: public
definition: https://synbiohub.org/public/igem/BBa_K1088003/1

BBa_K1088021
type: Component
persistent identity: component2373587
display id: component2373587
version: 1
top level: Rubber Fuc
access: public
definition: https://synbiohub.org/public/igem/BBa_K1088021/1

BBa_I13453
type: Component
persistent identity: component2373586
display id: component2373586
version: 1
top level: Rubber Fuc
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K1088025
type: Component
persistent identity: component2373592
display id: component2373592
version: 1
top level: Rubber Fuc
access: public
definition: https://synbiohub.org/public/igem/BBa_K1088025/1

BBa_J15001
type: Component
persistent identity: component2373590
display id: component2373590
version: 1
top level: Rubber Fuc
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2373586
display id: annotation2373586
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373586/range2373586/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373586/range2373586/1
type: Range
persistent identity: range2373586
display id: range2373586
version: 1
direction: direction/reverse
top level: Rubber Fuc
start: 1
end: 130
orientation: inline

BBa_K1088003
type: SequenceAnnotation
persistent identity: annotation2373591
display id: annotation2373591
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373591/range2373591/1
component: BBa_K1088003

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373591/range2373591/1
type: Range
persistent identity: range2373591
display id: range2373591
version: 1
direction: direction/reverse
top level: Rubber Fuc
start: 147
end: 1001
orientation: inline

BBa_K1088025
type: SequenceAnnotation
persistent identity: annotation2373592
display id: annotation2373592
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373592/range2373592/1
component: BBa_K1088025

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373592/range2373592/1
type: Range
persistent identity: range2373592
display id: range2373592
version: 1
direction: direction/reverse
top level: Rubber Fuc
start: 1002
end: 1067
orientation: inline

BBa_K1088021
type: SequenceAnnotation
persistent identity: annotation2373587
display id: annotation2373587
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373587/range2373587/1
component: BBa_K1088021

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373587/range2373587/1
type: Range
persistent identity: range2373587
display id: range2373587
version: 1
direction: direction/reverse
top level: Rubber Fuc
start: 131
end: 136
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2373590
display id: annotation2373590
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373590/range2373590/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373590/range2373590/1
type: Range
persistent identity: range2373590
display id: range2373590
version: 1
direction: direction/reverse
top level: Rubber Fuc
start: 137
end: 146
orientation: inline

BBa_K204047
type: ComponentDefinition
persistent identity: BBa K204047
display id: BBa_K204047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AI-1 generator
creator: Uno Keisuke
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _304_
m user id: 0
owner id: 5360
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: AI-1 generater p(tetR)+RBS+LasI+terminater
mutable notes: p(tetR)+RBS+LasI+terminater
mutable provenance: p(tetR)+RBS+LasI+terminater
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_R0040
component: BBa_C0078
component: BBa_B0034
sequence annotation: BBa_C0078
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K204047_sequence/1

BBa_B0015
type: Component
persistent identity: component2256017
display id: component2256017
version: 1
top level: BBa_K204047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2256000
display id: component2256000
version: 1
top level: BBa_K204047
access: public
definition: p(tetR)

BBa_C0078
type: Component
persistent identity: component2256010
display id: component2256010
version: 1
top level: BBa_K204047
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_B0034
type: Component
persistent identity: component2256006
display id: component2256006
version: 1
top level: BBa_K204047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2256010
display id: annotation2256010
version: 1
top level: BBa_K204047
location: https://synbiohub.org/public/igem/BBa_K204047/annotation2256010/range2256010/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K204047/annotation2256010/range2256010/1
type: Range
persistent identity: range2256010
display id: range2256010
version: 1
direction: direction/reverse
top level: BBa_K204047
start: 81
end: 747
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2256000
display id: annotation2256000
version: 1
top level: BBa_K204047
location: https://synbiohub.org/public/igem/BBa_K204047/annotation2256000/range2256000/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K204047/annotation2256000/range2256000/1
type: Range
persistent identity: range2256000
display id: range2256000
version: 1
direction: direction/forward
top level: BBa_K204047
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256006
display id: annotation2256006
version: 1
top level: BBa_K204047
location: https://synbiohub.org/public/igem/BBa_K204047/annotation2256006/range2256006/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K204047/annotation2256006/range2256006/1
type: Range
persistent identity: range2256006
display id: range2256006
version: 1
direction: direction/reverse
top level: BBa_K204047
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256017
display id: annotation2256017
version: 1
top level: BBa_K204047
location: https://synbiohub.org/public/igem/BBa_K204047/annotation2256017/range2256017/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K204047/annotation2256017/range2256017/1
type: Range
persistent identity: range2256017
display id: range2256017
version: 1
direction: direction/reverse
top level: BBa_K204047
start: 756
end: 884
orientation: inline

BBa_K530001
type: ComponentDefinition
persistent identity: BBa K530001
display id: BBa_K530001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CRTE
creator: Daniel Wolozny
created: 2011-07-31T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _696_
m user id: 0
owner id: 8447
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
mutable notes: Part was PCR amplified from the genomic DNA created from the experiment and paper: High-Level Production of Beta-Carotene in Saccharomyces Cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces Dendrorhous (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932764/).
mutable provenance: Found in the genomic sequence of Xanthophyllomyces Dendrorhous. http://www.ncbi.nlm.nih.gov/nuccore/AY177204.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Forward Primer
sequence annotation: Reverse Primer
sequence annotation: ORF
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K530001_sequence/1

Forward Primer
type: SequenceAnnotation
persistent identity: annotation2125795
display id: annotation2125795
version: 1
top level: BBa_K530001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K530001/annotation2125795/range2125795/1

https://synbiohub.org/public/igem/BBa_K530001/annotation2125795/range2125795/1
type: Range
persistent identity: range2125795
display id: range2125795
version: 1
direction: direction/forward
top level: BBa_K530001
start: 1
end: 25
orientation: inline

Reverse Primer
type: SequenceAnnotation
persistent identity: annotation2125796
display id: annotation2125796
version: 1
top level: BBa_K530001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K530001/annotation2125796/range2125796/1

https://synbiohub.org/public/igem/BBa_K530001/annotation2125796/range2125796/1
type: Range
persistent identity: range2125796
display id: range2125796
version: 1
direction: direction/reverse
top level: BBa_K530001
start: 1105
end: 1131
orientation: inline

ORF
type: SequenceAnnotation
persistent identity: annotation2125794
display id: annotation2125794
version: 1
top level: BBa_K530001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K530001/annotation2125794/range2125794/1

https://synbiohub.org/public/igem/BBa_K530001/annotation2125794/range2125794/1
type: Range
persistent identity: range2125794
display id: range2125794
version: 1
direction: direction/forward
top level: BBa_K530001
start: 1
end: 1131
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2125792
display id: annotation2125792
version: 1
top level: BBa_K530001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K530001/annotation2125792/range2125792/1

https://synbiohub.org/public/igem/BBa_K530001/annotation2125792/range2125792/1
type: Range
persistent identity: range2125792
display id: range2125792
version: 1
direction: direction/forward
top level: BBa_K530001
start: 1
end: 3
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2125793
display id: annotation2125793
version: 1
top level: BBa_K530001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K530001/annotation2125793/range2125793/1

https://synbiohub.org/public/igem/BBa_K530001/annotation2125793/range2125793/1
type: Range
persistent identity: range2125793
display id: range2125793
version: 1
direction: direction/forward
top level: BBa_K530001
start: 1129
end: 1131
orientation: inline

BBa_K360126
type: ComponentDefinition
persistent identity: BBa K360126
display id: BBa_K360126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23102 constitutive promoter + LovTAP Photosensor
creator: Claudia Ivonne Hernandez Armenta
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _485_
m user id: 0
owner id: 6618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LovTAP: Blue Light Photosensor and Transcriptional Regulator This photoreceptor was assembled by Strickland et al., and consists of a LOV (light-oxygen-voltage) domain of Avena sativa phototropin1 (AsLOV2) that senses blue light, fused to the trpR- DNA binding domain of the transcription factor trp repressor. The resulting protein is called LovTAP: LOV- and tryptophan-activated protein. LOV domains bind a flavin-mononucleotide (FMN) or flavin-adenine-dinucleotide (FAD) cofactor, which are used in a wide variety of metabolic pathways as cofactors in redox reactions and are available in most organisms. The cofactor has a broad absorption spectrum, with a maximum at 450 nm. Besides, the core of the LOV domain is often flanked by amino- or carboxy-terminal helices, termed A???α and Jα, respectively. In the LovTAP construction, AsLOV2 domain via its carboxyl-terminal Jα ???helix was ligated to an amino-terminal truncation of TrpR. The resulting protein has a domain-domain overlap with a shared helix. Thus, photoexcitation would change the conformation of the protein, in turn changing the stability of the shared-helix-domain contacts. Under the presence of light, absorption of a photon leads to the formation of a covalent adduct between the flavin mononucleotide (FMN) cofactor and a conserved cysteine residue in the AsLOV2 domain, which results in conformational rearrangements in LovTAP. This change impacts the affinity of the shared helix for the two domains: disrupting the contacts between the shared helix and the LOV domain and enabling the association of the shared helix with the TrpR domain, which establishes DNA-binding affinity and LovTAP can then bind DNA as an homodimer, repressing the transcription of the genes downstream of its binding sites. In the dark, when the shared helix contacts the LOV domain, the TrpR domain's DNA-binding affinity decreases and LovTAP is in an inactive conformation.
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K360121
component: BBa_J23102
sequence annotation: BBa_J23102
sequence annotation: BBa_K360121
sequence: https://synbiohub.org/public/igem/BBa_K360126_sequence/1

BBa_K360121
type: Component
persistent identity: component2091858
display id: component2091858
version: 1
top level: BBa_K360126
access: public
definition: BBa_K360121

BBa_J23102
type: Component
persistent identity: component2091851
display id: component2091851
version: 1
top level: BBa_K360126
access: public
definition: https://synbiohub.org/public/igem/BBa_J23102/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2091851
display id: annotation2091851
version: 1
top level: BBa_K360126
location: https://synbiohub.org/public/igem/BBa_K360126/annotation2091851/range2091851/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K360126/annotation2091851/range2091851/1
type: Range
persistent identity: range2091851
display id: range2091851
version: 1
direction: direction/reverse
top level: BBa_K360126
start: 1
end: 35
orientation: inline

BBa_K360121
type: SequenceAnnotation
persistent identity: annotation2091858
display id: annotation2091858
version: 1
top level: BBa_K360126
location: https://synbiohub.org/public/igem/BBa_K360126/annotation2091858/range2091858/1
component: BBa_K360121

https://synbiohub.org/public/igem/BBa_K360126/annotation2091858/range2091858/1
type: Range
persistent identity: range2091858
display id: range2091858
version: 1
direction: direction/reverse
top level: BBa_K360126
start: 44
end: 887
orientation: inline

BAT2
type: ComponentDefinition
persistent identity: BBa J45008
display id: BBa_J45008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
creator: Veena Venkatachalam
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: fd;lsfas
mutable notes: dakfdas;
mutable provenance: dkfkas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: BAT2
sequence annotation: double TAA stop codon
sequence annotation: a->g to eliminate SpeI
sequence: https://synbiohub.org/public/igem/BBa_J45008_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1883624
display id: annotation1883624
version: 1
top level: BAT2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1
type: Range
persistent identity: range1883624
display id: range1883624
version: 1
direction: direction/forward
top level: BAT2
start: 1
end: 3
orientation: inline

BAT2
type: SequenceAnnotation
persistent identity: annotation1961299
display id: annotation1961299
version: 1
top level: BAT2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1
type: Range
persistent identity: range1961299
display id: range1961299
version: 1
direction: direction/forward
top level: BAT2
start: 1
end: 1134
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1883625
display id: annotation1883625
version: 1
top level: BAT2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1
type: Range
persistent identity: range1883625
display id: range1883625
version: 1
direction: direction/forward
top level: BAT2
start: 1129
end: 1134
orientation: inline

a->g to eliminate SpeI
type: SequenceAnnotation
persistent identity: annotation1894553
display id: annotation1894553
version: 1
top level: BAT2
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1
type: Range
persistent identity: range1894553
display id: range1894553
version: 1
direction: direction/forward
top level: BAT2
start: 771
end: 771
orientation: inline

mCh
type: ComponentDefinition
persistent identity: BBa J176005
display id: BBa_J176005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCh
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: Humanized mCherry fluorescent protein
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176005_sequence/1

BBa_K137007
type: ComponentDefinition
persistent identity: BBa K137007
display id: BBa_K137007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fimE
creator: Allen Lin
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: fimE gene
mutable notes: none
mutable provenance: pFIP plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K137007_sequence/1

BBa_K098995
type: ComponentDefinition
persistent identity: BBa K098995
display id: BBa_K098995
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: heat sensitive cI QPI with high promoter
creator: Harvard iGEM 08
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a heat sensitive cI QPI with a high promoter preceding the repressor (BBa_J23114). It can be used to construct a heat-inducible system if a reporter or indicator is adding to the right of this part.
mutable notes: This is a mutagenized form because the original product had an extra PstI site between the repressor and terminator. While there are still extra basepairs between the end of the repressor sequence and the Biobricks scar, they no longer code for a PstI site. The extra base pairs are: [repressor coding region] CTCCAGCGGCCGC [Biobricks scar]
mutable provenance: PGW7 plasmid and various BioBricks parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S04137
component: BBa_J23114
sequence annotation: BBa_J23114
sequence annotation: BBa_S04137
sequence: https://synbiohub.org/public/igem/BBa_K098995_sequence/1

BBa_S04137
type: Component
persistent identity: component2224150
display id: component2224150
version: 1
top level: BBa_K098995
access: public
definition: https://synbiohub.org/public/igem/BBa_S04137/1

BBa_J23114
type: Component
persistent identity: component2224132
display id: component2224132
version: 1
top level: BBa_K098995
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2224132
display id: annotation2224132
version: 1
top level: BBa_K098995
location: https://synbiohub.org/public/igem/BBa_K098995/annotation2224132/range2224132/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K098995/annotation2224132/range2224132/1
type: Range
persistent identity: range2224132
display id: range2224132
version: 1
direction: direction/reverse
top level: BBa_K098995
start: 1
end: 35
orientation: inline

BBa_S04137
type: SequenceAnnotation
persistent identity: annotation2224150
display id: annotation2224150
version: 1
top level: BBa_K098995
location: https://synbiohub.org/public/igem/BBa_K098995/annotation2224150/range2224150/1
component: BBa_S04137

https://synbiohub.org/public/igem/BBa_K098995/annotation2224150/range2224150/1
type: Range
persistent identity: range2224150
display id: range2224150
version: 1
direction: direction/reverse
top level: BBa_K098995
start: 44
end: 929
orientation: inline

ARL2A0404
type: ComponentDefinition
persistent identity: BBa I732114
display id: BBa_I732114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0404
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732114_sequence/1

BBa_K1189008
type: ComponentDefinition
persistent identity: BBa K1189008
display id: BBa_K1189008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-Lactamase
creator: Somshukla Chaudhuri
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1502_
m user id: 0
owner id: 13453
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: true
mutable description: adaskd
mutable notes: adskajd
mutable provenance: asaksjd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K1189008_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2365963
display id: annotation2365963
version: 1
top level: BBa_K1189008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1189008/annotation2365963/range2365963/1

https://synbiohub.org/public/igem/BBa_K1189008/annotation2365963/range2365963/1
type: Range
persistent identity: range2365963
display id: range2365963
version: 1
direction: direction/forward
top level: BBa_K1189008
start: 1
end: 3
orientation: inline

CMVTetO2
type: ComponentDefinition
persistent identity: BBa J176017
display id: BBa_J176017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMVTetO2
creator: Brady Laughlin
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: complete tet./ dox. inducible promoter from Invitrogen vector pcDNA/FRT/TO, T-Rex system
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetO x2
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_J176017_sequence/1

TetO x2
type: SequenceAnnotation
persistent identity: annotation2147356
display id: annotation2147356
version: 1
top level: CMVTetO2
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J176017/annotation2147356/range2147356/1

https://synbiohub.org/public/igem/BBa_J176017/annotation2147356/range2147356/1
type: Range
persistent identity: range2147356
display id: range2147356
version: 1
direction: direction/forward
top level: CMVTetO2
start: 589
end: 628
orientation: inline

TATA box
type: SequenceAnnotation
persistent identity: annotation2147355
display id: annotation2147355
version: 1
top level: CMVTetO2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176017/annotation2147355/range2147355/1

https://synbiohub.org/public/igem/BBa_J176017/annotation2147355/range2147355/1
type: Range
persistent identity: range2147355
display id: range2147355
version: 1
direction: direction/forward
top level: CMVTetO2
start: 573
end: 579
orientation: inline

BBa_K141002
type: ComponentDefinition
persistent identity: BBa K141002
display id: BBa_K141002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ucp 175 deleted
creator: Jaume For??s Martos
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _229_
m user id: 0
owner id: 2947
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K141002_sequence/1

BBa_K199118
type: ComponentDefinition
persistent identity: BBa K199118
display id: BBa_K199118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter + RBS + RFP
creator: Clifton Davis
created: 2010-03-02T12:00:00Z
modified: 2015-05-08T01:11:20Z
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5118
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 Promoter (R0085) + RBS (B0034) + RFP (E1010)
mutable notes: N/A
mutable provenance: Biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0085
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0085
sequence: https://synbiohub.org/public/igem/BBa_K199118_sequence/1

BBa_R0085
type: Component
persistent identity: component2247068
display id: component2247068
version: 1
top level: BBa_K199118
access: public
definition: https://synbiohub.org/public/igem/BBa_R0085/1

BBa_B0034
type: Component
persistent identity: component2247070
display id: component2247070
version: 1
top level: BBa_K199118
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2247073
display id: component2247073
version: 1
top level: BBa_K199118
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247073
display id: annotation2247073
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247073/range2247073/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K199118/annotation2247073/range2247073/1
type: Range
persistent identity: range2247073
display id: range2247073
version: 1
direction: direction/reverse
top level: BBa_K199118
start: 50
end: 755
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2247070
display id: annotation2247070
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247070/range2247070/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K199118/annotation2247070/range2247070/1
type: Range
persistent identity: range2247070
display id: range2247070
version: 1
direction: direction/reverse
top level: BBa_K199118
start: 32
end: 43
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation2247068
display id: annotation2247068
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247068/range2247068/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_K199118/annotation2247068/range2247068/1
type: Range
persistent identity: range2247068
display id: range2247068
version: 1
direction: direction/reverse
top level: BBa_K199118
start: 1
end: 23
orientation: inline

BBa_K899012
type: ComponentDefinition
persistent identity: BBa K899012
display id: BBa_K899012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SST (BBa_K899001) with double RBS (BBa_K899010)
creator: Andreas Kj??r
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K899000
component: BBa_K899010
sequence annotation: BBa_K899000
sequence annotation: BBa_K899010
sequence: https://synbiohub.org/public/igem/BBa_K899012_sequence/1

BBa_K899000
type: Component
persistent identity: component2202323
display id: component2202323
version: 1
top level: BBa_K899012
access: public
definition: https://synbiohub.org/public/igem/BBa_K899000/1

BBa_K899010
type: Component
persistent identity: component2202320
display id: component2202320
version: 1
top level: BBa_K899012
access: public
definition: https://synbiohub.org/public/igem/BBa_K899010/1

BBa_K899000
type: SequenceAnnotation
persistent identity: annotation2202323
display id: annotation2202323
version: 1
top level: BBa_K899012
location: https://synbiohub.org/public/igem/BBa_K899012/annotation2202323/range2202323/1
component: BBa_K899000

https://synbiohub.org/public/igem/BBa_K899012/annotation2202323/range2202323/1
type: Range
persistent identity: range2202323
display id: range2202323
version: 1
direction: direction/reverse
top level: BBa_K899012
start: 20
end: 1912
orientation: inline

BBa_K899010
type: SequenceAnnotation
persistent identity: annotation2202320
display id: annotation2202320
version: 1
top level: BBa_K899012
location: https://synbiohub.org/public/igem/BBa_K899012/annotation2202320/range2202320/1
component: BBa_K899010

https://synbiohub.org/public/igem/BBa_K899012/annotation2202320/range2202320/1
type: Range
persistent identity: range2202320
display id: range2202320
version: 1
direction: direction/reverse
top level: BBa_K899012
start: 1
end: 13
orientation: inline

BBa_K204500
type: ComponentDefinition
persistent identity: BBa K204500
display id: BBa_K204500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FliH
creator: Uno Keisuke
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
group u list: _304_
m user id: 0
owner id: 5360
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: FliH
mutable notes: FliH
mutable provenance: FliH
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FliH
sequence: https://synbiohub.org/public/igem/BBa_K204500_sequence/1

FliH
type: SequenceAnnotation
persistent identity: annotation2042760
display id: annotation2042760
version: 1
top level: BBa_K204500
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K204500/annotation2042760/range2042760/1

https://synbiohub.org/public/igem/BBa_K204500/annotation2042760/range2042760/1
type: Range
persistent identity: range2042760
display id: range2042760
version: 1
direction: direction/forward
top level: BBa_K204500
start: 1
end: 708
orientation: inline

BBa_K899011
type: ComponentDefinition
persistent identity: BBa K899011
display id: BBa_K899011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FFT (BBa_K899001) with double RBS (BBa_K899009)
creator: Andreas Kj??r
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a composite part containing our fructan:fructan fructosyltransferase (FFT) gene with a "double" RBS containing both the shine-dalgarno and kozak sequence. This is the main part of our project, and also what we submitted to iGEM. The part was characterized in a pJET vector (http://www.bioinfo.pte.hu/f2/pict_f2/pJETmap.pdf) and we found a weird looking substance that resembled sugar structure in the growth medium. When we expressed FFT in E. coli top 10 strains they grew as fast as regular top 10 bacteria, probably because the sucrose:sucrose fructosyltransferase (SST) enzyme wasnt present (this enzyme produces the base that FFT polymerizes). When staining for inulin we observed a slight increase in inulin even though the SST enzyme wasn't present. This is probably because there was something in the substrate that enabled FFT to polymerize the sugar.
mutable notes: none
mutable provenance: The FFT gene derives from the jerusalem artichoke (Helianthus Tuberosus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K899009
component: BBa_K899001
sequence annotation: BBa_K899009
sequence annotation: BBa_K899001
sequence: https://synbiohub.org/public/igem/BBa_K899011_sequence/1

BBa_K899009
type: Component
persistent identity: component2202166
display id: component2202166
version: 1
top level: BBa_K899011
access: public
definition: https://synbiohub.org/public/igem/BBa_K899009/1

BBa_K899001
type: Component
persistent identity: component2202169
display id: component2202169
version: 1
top level: BBa_K899011
access: public
definition: BBa_K899001

BBa_K899009
type: SequenceAnnotation
persistent identity: annotation2202166
display id: annotation2202166
version: 1
top level: BBa_K899011
location: https://synbiohub.org/public/igem/BBa_K899011/annotation2202166/range2202166/1
component: BBa_K899009

https://synbiohub.org/public/igem/BBa_K899011/annotation2202166/range2202166/1
type: Range
persistent identity: range2202166
display id: range2202166
version: 1
direction: direction/reverse
top level: BBa_K899011
start: 1
end: 13
orientation: inline

BBa_K899001
type: SequenceAnnotation
persistent identity: annotation2202169
display id: annotation2202169
version: 1
top level: BBa_K899011
location: https://synbiohub.org/public/igem/BBa_K899011/annotation2202169/range2202169/1
component: BBa_K899001

https://synbiohub.org/public/igem/BBa_K899011/annotation2202169/range2202169/1
type: Range
persistent identity: range2202169
display id: range2202169
version: 1
direction: direction/reverse
top level: BBa_K899011
start: 20
end: 1867
orientation: inline

AAR
type: ComponentDefinition
persistent identity: BBa K590032
display id: BBa_K590032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942
creator: Lei Zheng
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Produces enzyme that reduces long chain length acyl-ACPs into the corresponding fatty aldehydes
mutable notes: None
mutable provenance: Ordered from GenScript
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K590032_sequence/1

lsrK
type: ComponentDefinition
persistent identity: BBa K091002
display id: BBa_K091002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrK gene
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part codes for the protein LsrK which phosphorylates AI-2 which acts to promote the lsrA promoter.
mutable notes: no special considerations
mutable provenance: This sequence is found in the E. coli strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091002_sequence/1

BBa_K141000
type: ComponentDefinition
persistent identity: BBa K141000
display id: BBa_K141000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ucp1
creator: Jaume For??s Martos
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _229_
m user id: 0
owner id: 2947
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Ucp1 is a DNA part which encodes for a protein called UCP1 which belongs to the uncuopling proteins family. The uncoupling proteins (UCPs) are transporters, presents in the mitochondrial inner membrane, that mediate a regulated discharge of the proton gradient that is generated by the respiratory chain. This energy-dissipatory mechanism can serve functions such as termogenesis. The protein is regulated by acivation and inhibition in the mitochondrion. The proton conductance in UCP1 is inhibited by nucleotides and activated by fatty acids.
mutable notes: none
mutable provenance: Uncouplin protein UCP1 is a protein that is found in the mitochondria from brown adipocytes.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K141000_sequence/1

BBa_K500001
type: ComponentDefinition
persistent identity: BBa K500001
display id: BBa_K500001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lignin degradation 2
creator: Bin Jia
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _596_
m user id: 0
owner id: 4808
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: substrate：phenols
mutable notes: no
mutable provenance: P.chrysoporium
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PRO
sequence annotation: MNP
sequence: https://synbiohub.org/public/igem/BBa_K500001_sequence/1

PRO
type: SequenceAnnotation
persistent identity: annotation2104160
display id: annotation2104160
version: 1
top level: BBa_K500001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K500001/annotation2104160/range2104160/1

https://synbiohub.org/public/igem/BBa_K500001/annotation2104160/range2104160/1
type: Range
persistent identity: range2104160
display id: range2104160
version: 1
direction: direction/forward
top level: BBa_K500001
start: 1
end: 3
orientation: inline

MNP
type: SequenceAnnotation
persistent identity: annotation2109133
display id: annotation2109133
version: 1
top level: BBa_K500001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K500001/annotation2109133/range2109133/1

https://synbiohub.org/public/igem/BBa_K500001/annotation2109133/range2109133/1
type: Range
persistent identity: range2109133
display id: range2109133
version: 1
direction: direction/forward
top level: BBa_K500001
start: 4
end: 1134
orientation: inline

BBa_P10402
type: ComponentDefinition
persistent identity: BBa P10402
display id: BBa_P10402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: G7
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T06:04:04Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP72400
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10402_sequence/1

Fok_a-Venu
type: ComponentDefinition
persistent identity: BBa K243032
display id: BBa_K243032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fok_a-Venus
creator: Freiburg Bioware09
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is our protein domain Fok_a from our universal endonuclease, which we linked with an YFP-part.
mutable notes: Designed according the RFC25
mutable provenance: Combiantion of our prtein domain Fok_a and the mVenus-YFP_FusionPartfrom the Team Freiburg07
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I757008
component: BBa_K243000
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K243000
sequence annotation: BBa_I757008
sequence: https://synbiohub.org/public/igem/BBa_K243032_sequence/1

BBa_I757008
type: Component
persistent identity: component2055455
display id: component2055455
version: 1
top level: Fok_a-Venu
access: public
definition: https://synbiohub.org/public/igem/BBa_I757008/1

BBa_K243000
type: Component
persistent identity: component2055452
display id: component2055452
version: 1
top level: Fok_a-Venu
access: public
definition: Fok_a

BBa_B0105
type: Component
persistent identity: component2055454
display id: component2055454
version: 1
top level: Fok_a-Venu
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2055454
display id: annotation2055454
version: 1
top level: Fok_a-Venu
location: https://synbiohub.org/public/igem/BBa_K243032/annotation2055454/range2055454/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K243032/annotation2055454/range2055454/1
type: Range
persistent identity: range2055454
display id: range2055454
version: 1
direction: direction/reverse
top level: Fok_a-Venu
start: 580
end: 585
orientation: inline

BBa_K243000
type: SequenceAnnotation
persistent identity: annotation2055452
display id: annotation2055452
version: 1
top level: Fok_a-Venu
location: https://synbiohub.org/public/igem/BBa_K243032/annotation2055452/range2055452/1
component: BBa_K243000

https://synbiohub.org/public/igem/BBa_K243032/annotation2055452/range2055452/1
type: Range
persistent identity: range2055452
display id: range2055452
version: 1
direction: direction/reverse
top level: Fok_a-Venu
start: 1
end: 579
orientation: inline

BBa_I757008
type: SequenceAnnotation
persistent identity: annotation2055455
display id: annotation2055455
version: 1
top level: Fok_a-Venu
location: https://synbiohub.org/public/igem/BBa_K243032/annotation2055455/range2055455/1
component: BBa_I757008

https://synbiohub.org/public/igem/BBa_K243032/annotation2055455/range2055455/1
type: Range
persistent identity: range2055455
display id: range2055455
version: 1
direction: direction/reverse
top level: Fok_a-Venu
start: 586
end: 1299
orientation: inline

BBa_K346001
type: ComponentDefinition
persistent identity: BBa K346001
display id: BBa_K346001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (B0034) + MerR (mercury-responsive transcription factor)
creator: Ao Liu & Ying Sheng
created: 2010-10-07T11:00:00Z
modified: 2015-07-21T02:53:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_457_
m user id: 4206
owner id: 4411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part was designed as a translational unit for MerR expression.
mutable notes: The native RBS of MerR is not strong enough and its intensity can not be predicted as there is no such a corresponding RBS part in Registry. In order to facilitate the use and further characterization of MerR for future team, we prefixed an RBS part BBa_B0034 from Partsregistry.
mutable provenance: The coding sequence of MerR comes from Tn, prefixed by an RBS part BBa_B0034 from Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: B0034
sequence annotation: merR
sequence: https://synbiohub.org/public/igem/BBa_K346001_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2093291
display id: annotation2093291
version: 1
top level: BBa_K346001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K346001/annotation2093291/range2093291/1

https://synbiohub.org/public/igem/BBa_K346001/annotation2093291/range2093291/1
type: Range
persistent identity: range2093291
display id: range2093291
version: 1
direction: direction/forward
top level: BBa_K346001
start: 1
end: 12
orientation: inline

merR
type: SequenceAnnotation
persistent identity: annotation2093295
display id: annotation2093295
version: 1
top level: BBa_K346001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346001/annotation2093295/range2093295/1

https://synbiohub.org/public/igem/BBa_K346001/annotation2093295/range2093295/1
type: Range
persistent identity: range2093295
display id: range2093295
version: 1
direction: direction/forward
top level: BBa_K346001
start: 19
end: 453
orientation: inline

BBa_K914008
type: ComponentDefinition
persistent identity: BBa K914008
display id: BBa_K914008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Meganuclease I-SceI controlled by pRha
creator: Denis Samuylov
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1179_
m user id: 0
owner id: 13487
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: I-SceI homing endonuclease expression is controlled by pRha promoter. Expression is induced with L-rhamnose. I-SceI doesn't have an LVA degradation tag.
mutable notes: The system is leaky and meganuclease cut RS with high efficiency even without pRha induction.
mutable provenance: Promoter pRha was amplified from the plasmid pJOE3075 (Dr. Altenbuchner). RBS was taken from an iGEM distribution plate (BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K914006
component: BBa_B0032
component: BBa_K914003
sequence annotation: BBa_K914003
sequence annotation: BBa_B0032
sequence annotation: BBa_K914006
sequence: https://synbiohub.org/public/igem/BBa_K914008_sequence/1

BBa_K914006
type: Component
persistent identity: component2188838
display id: component2188838
version: 1
top level: BBa_K914008
access: public
definition: https://synbiohub.org/public/igem/BBa_K914006/1

BBa_B0032
type: Component
persistent identity: component2188835
display id: component2188835
version: 1
top level: BBa_K914008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K914003
type: Component
persistent identity: component2188833
display id: component2188833
version: 1
top level: BBa_K914008
access: public
definition: https://synbiohub.org/public/igem/BBa_K914003/1

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2188833
display id: annotation2188833
version: 1
top level: BBa_K914008
location: https://synbiohub.org/public/igem/BBa_K914008/annotation2188833/range2188833/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K914008/annotation2188833/range2188833/1
type: Range
persistent identity: range2188833
display id: range2188833
version: 1
direction: direction/reverse
top level: BBa_K914008
start: 1
end: 122
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2188835
display id: annotation2188835
version: 1
top level: BBa_K914008
location: https://synbiohub.org/public/igem/BBa_K914008/annotation2188835/range2188835/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K914008/annotation2188835/range2188835/1
type: Range
persistent identity: range2188835
display id: range2188835
version: 1
direction: direction/forward
top level: BBa_K914008
start: 131
end: 143
orientation: inline

BBa_K914006
type: SequenceAnnotation
persistent identity: annotation2188838
display id: annotation2188838
version: 1
top level: BBa_K914008
location: https://synbiohub.org/public/igem/BBa_K914008/annotation2188838/range2188838/1
component: BBa_K914006

https://synbiohub.org/public/igem/BBa_K914008/annotation2188838/range2188838/1
type: Range
persistent identity: range2188838
display id: range2188838
version: 1
direction: direction/reverse
top level: BBa_K914008
start: 150
end: 861
orientation: inline

luc
type: ComponentDefinition
persistent identity: BBa K389004
display id: BBa_K389004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luciferase from pGL4.10[luc2]
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A luciferase from Promega's pGL4.10luc2 vector.
mutable notes: sequence of this vector is already published by Promega
mutable provenance: pGL4.10luc2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Luciferase
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389004_sequence/1

Luciferase
type: SequenceAnnotation
persistent identity: annotation2077057
display id: annotation2077057
version: 1
top level: luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389004/annotation2077057/range2077057/1

https://synbiohub.org/public/igem/BBa_K389004/annotation2077057/range2077057/1
type: Range
persistent identity: range2077057
display id: range2077057
version: 1
direction: direction/forward
top level: luc
start: 1
end: 1653
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078364
display id: annotation2078364
version: 1
top level: luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389004/annotation2078364/range2078364/1

https://synbiohub.org/public/igem/BBa_K389004/annotation2078364/range2078364/1
type: Range
persistent identity: range2078364
display id: range2078364
version: 1
direction: direction/forward
top level: luc
start: 1654
end: 1656
orientation: inline

BBa_P10101
type: ComponentDefinition
persistent identity: BBa P10101
display id: BBa_P10101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s cauliflower mosaic virus
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:51:02Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP41373
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10101_sequence/1

BBa_J36846
type: ComponentDefinition
persistent identity: BBa J36846
display id: BBa_J36846
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA(46-159)-Streptavidin wild-type + His6
creator: Perry Tsai
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1229
owning group id: 65
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Assembled construct of signal peptide of lipoprotein, five transmembrane domains of outer membrane protein A, and streptavidin, wild-type with His6 tag at the end. This construct codes for a protein which displays streptavidin on the cell surface.
mutable notes: There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains. Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.
mutable provenance: Assembly of J36835, J36837, J36841
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J36837
component: BBa_J36835
component: BBa_J36841
sequence annotation: BBa_J36835
sequence annotation: BBa_J36841
sequence annotation: BBa_J36837
sequence: https://synbiohub.org/public/igem/BBa_J36846_sequence/1

BBa_J36837
type: Component
persistent identity: component1909024
display id: component1909024
version: 1
top level: BBa_J36846
access: public
definition: https://synbiohub.org/public/igem/BBa_J36837/1

BBa_J36835
type: Component
persistent identity: component1909023
display id: component1909023
version: 1
top level: BBa_J36846
access: public
definition: https://synbiohub.org/public/igem/BBa_J36835/1

BBa_J36841
type: Component
persistent identity: component1909025
display id: component1909025
version: 1
top level: BBa_J36846
access: public
definition: https://synbiohub.org/public/igem/BBa_J36841/1

BBa_J36835
type: SequenceAnnotation
persistent identity: annotation1909023
display id: annotation1909023
version: 1
top level: BBa_J36846
location: https://synbiohub.org/public/igem/BBa_J36846/annotation1909023/range1909023/1
component: BBa_J36835

https://synbiohub.org/public/igem/BBa_J36846/annotation1909023/range1909023/1
type: Range
persistent identity: range1909023
display id: range1909023
version: 1
direction: direction/reverse
top level: BBa_J36846
start: 1
end: 87
orientation: inline

BBa_J36841
type: SequenceAnnotation
persistent identity: annotation1909025
display id: annotation1909025
version: 1
top level: BBa_J36846
location: https://synbiohub.org/public/igem/BBa_J36846/annotation1909025/range1909025/1
component: BBa_J36841

https://synbiohub.org/public/igem/BBa_J36846/annotation1909025/range1909025/1
type: Range
persistent identity: range1909025
display id: range1909025
version: 1
direction: direction/reverse
top level: BBa_J36846
start: 446
end: 850
orientation: inline

BBa_J36837
type: SequenceAnnotation
persistent identity: annotation1909024
display id: annotation1909024
version: 1
top level: BBa_J36846
location: https://synbiohub.org/public/igem/BBa_J36846/annotation1909024/range1909024/1
component: BBa_J36837

https://synbiohub.org/public/igem/BBa_J36846/annotation1909024/range1909024/1
type: Range
persistent identity: range1909024
display id: range1909024
version: 1
direction: direction/reverse
top level: BBa_J36846
start: 96
end: 437
orientation: inline

BBa_J97003
type: ComponentDefinition
persistent identity: BBa J97003
display id: BBa_J97003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IP-Free TannenRFP (Red Fluorescent Protein)
creator: Drew Endy
created: 2014-02-18T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: x
mutable notes: This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
mutable provenance: This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97003_sequence/1

ARL2A0204
type: ComponentDefinition
persistent identity: BBa I732111
display id: BBa_I732111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0204
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732111_sequence/1

BBa_K197019
type: ComponentDefinition
persistent identity: BBa K197019
display id: BBa_K197019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TypeIII Needle scFv
creator: Gabriela Guzman Lopez Aguado
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _294_
m user id: 0
owner id: 5247
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This antibody detects the espA motif of the typeIII needle complex present in many pathogenic bacteria. This can be surface displayed for pathogen detection applciations.
mutable notes: n/a
mutable provenance: gene synthesized, sequence taken from La Ragione, R et al. Recombinant anti-EspA antibodies block Escherichia coli O157:H7-induced attaching and effacing lesions in vitro. Microbes and Infection. September 2005; 8: 426???433. Available Online: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VPN-4H4T215-1-7&_cdi=6211&_user=4420&_orig=search&_coverDate=02%2F28%2F2006&_sk=999919997&view=c&wchp=dGLbVtz-zSkzS&md5=f35699db5dcd202f4cd1c1d8739d89b0&ie=/sdarticle.pdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K197019_sequence/1

BBa_P10401
type: ComponentDefinition
persistent identity: BBa P10401
display id: BBa_P10401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nos
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T06:03:01Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP41421
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10401_sequence/1

BBa_K427005
type: ComponentDefinition
persistent identity: BBa K427005
display id: BBa_K427005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MuC sensitivity tuner (PoPS->PoPS)
creator: Jan Marte Contreras Ortiz
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a sensitivity tuner created form the C protein and Pmom promoter of the Mu bacteriophage it can be used to increase the PoPS of a construct. The C protein encoded in the first part of the construct is the activator of the Pmom promoter, which does not begin transcription until the C protein binds to its consensus sequence and attracts the polymerase.
mutable notes: No special design considerations
mutable provenance: We built this part form C and Pmom sequences of Mu bacetriophage and included the bidirectional terminator BBa_B0014 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K427004
component: BBa_B0014
component: BBa_K427001
sequence annotation: BBa_B0014
sequence annotation: BBa_K427001
sequence annotation: BBa_K427004
sequence: https://synbiohub.org/public/igem/BBa_K427005_sequence/1

BBa_K427004
type: Component
persistent identity: component2092384
display id: component2092384
version: 1
top level: BBa_K427005
access: public
definition: https://synbiohub.org/public/igem/BBa_K427004/1

BBa_B0014
type: Component
persistent identity: component2092382
display id: component2092382
version: 1
top level: BBa_K427005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K427001
type: Component
persistent identity: component2092375
display id: component2092375
version: 1
top level: BBa_K427005
access: public
definition: https://synbiohub.org/public/igem/BBa_K427001/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092382
display id: annotation2092382
version: 1
top level: BBa_K427005
location: https://synbiohub.org/public/igem/BBa_K427005/annotation2092382/range2092382/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K427005/annotation2092382/range2092382/1
type: Range
persistent identity: range2092382
display id: range2092382
version: 1
direction: direction/reverse
top level: BBa_K427005
start: 447
end: 541
orientation: inline

BBa_K427001
type: SequenceAnnotation
persistent identity: annotation2092375
display id: annotation2092375
version: 1
top level: BBa_K427005
location: https://synbiohub.org/public/igem/BBa_K427005/annotation2092375/range2092375/1
component: BBa_K427001

https://synbiohub.org/public/igem/BBa_K427005/annotation2092375/range2092375/1
type: Range
persistent identity: range2092375
display id: range2092375
version: 1
direction: direction/reverse
top level: BBa_K427005
start: 1
end: 438
orientation: inline

BBa_K427004
type: SequenceAnnotation
persistent identity: annotation2092384
display id: annotation2092384
version: 1
top level: BBa_K427005
location: https://synbiohub.org/public/igem/BBa_K427005/annotation2092384/range2092384/1
component: BBa_K427004

https://synbiohub.org/public/igem/BBa_K427005/annotation2092384/range2092384/1
type: Range
persistent identity: range2092384
display id: range2092384
version: 1
direction: direction/reverse
top level: BBa_K427005
start: 550
end: 628
orientation: inline

"G"
type: ComponentDefinition
persistent identity: BBa K1080009
display id: BBa_K1080009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ChlG
creator: Macquarie University
created: 2013-09-21T11:00:00Z
modified: 2016-10-20T07:21:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 7708
owner id: 18612
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: FSDBSDGBGDS
mutable notes: DFSHGDSS
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: ChlG
sequence: https://synbiohub.org/public/igem/BBa_K1080009_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2525691
display id: annotation2525691
version: 1
top level: "G"
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1080009/annotation2525691/range2525691/1

https://synbiohub.org/public/igem/BBa_K1080009/annotation2525691/range2525691/1
type: Range
persistent identity: range2525691
display id: range2525691
version: 1
direction: direction/forward
top level: "G"
start: 6
end: 16
orientation: inline

ChlG
type: SequenceAnnotation
persistent identity: annotation2525692
display id: annotation2525692
version: 1
top level: "G"
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1080009/annotation2525692/range2525692/1

https://synbiohub.org/public/igem/BBa_K1080009/annotation2525692/range2525692/1
type: Range
persistent identity: range2525692
display id: range2525692
version: 1
direction: direction/forward
top level: "G"
start: 25
end: 1044
orientation: inline

EFE
type: ComponentDefinition
persistent identity: BBa K1065000
display id: BBa_K1065000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 2-oxoglutarate oxygenase/decarboxylase (EFE)
creator: Thomas Perli
created: 2013-06-19T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1373_
m user id: 0
owner id: 15969
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: xxx
mutable notes: xxx
mutable provenance: xxxx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_K1065000
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: CDS - Ethylene Forming Enzyme
sequence annotation: RBS
sequence annotation: Spacer
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K1065000_sequence/1

BBa_K1065000
type: SequenceAnnotation
persistent identity: annotation2331673
display id: annotation2331673
version: 1
top level: EFE
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331673/range2331673/1

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331673/range2331673/1
type: Range
persistent identity: range2331673
display id: range2331673
version: 1
direction: direction/reverse
top level: EFE
start: 1
end: 1078
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2331678
display id: annotation2331678
version: 1
top level: EFE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331678/range2331678/1

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331678/range2331678/1
type: Range
persistent identity: range2331678
display id: range2331678
version: 1
direction: direction/forward
top level: EFE
start: 1070
end: 1075
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2331677
display id: annotation2331677
version: 1
top level: EFE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331677/range2331677/1

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331677/range2331677/1
type: Range
persistent identity: range2331677
display id: range2331677
version: 1
direction: direction/forward
top level: EFE
start: 17
end: 22
orientation: inline

CDS - Ethylene Forming Enzyme
type: SequenceAnnotation
persistent identity: annotation2331675
display id: annotation2331675
version: 1
top level: EFE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331675/range2331675/1

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331675/range2331675/1
type: Range
persistent identity: range2331675
display id: range2331675
version: 1
direction: direction/forward
top level: EFE
start: 14
end: 1078
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2331672
display id: annotation2331672
version: 1
top level: EFE
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331672/range2331672/1

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331672/range2331672/1
type: Range
persistent identity: range2331672
display id: range2331672
version: 1
direction: direction/forward
top level: EFE
start: 1
end: 6
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2331674
display id: annotation2331674
version: 1
top level: EFE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331674/range2331674/1

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331674/range2331674/1
type: Range
persistent identity: range2331674
display id: range2331674
version: 1
direction: direction/forward
top level: EFE
start: 7
end: 13
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2331676
display id: annotation2331676
version: 1
top level: EFE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331676/range2331676/1

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331676/range2331676/1
type: Range
persistent identity: range2331676
display id: range2331676
version: 1
direction: direction/forward
top level: EFE
start: 14
end: 16
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2331679
display id: annotation2331679
version: 1
top level: EFE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331679/range2331679/1

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331679/range2331679/1
type: Range
persistent identity: range2331679
display id: range2331679
version: 1
direction: direction/forward
top level: EFE
start: 1076
end: 1078
orientation: inline

BBa_K228236
type: ComponentDefinition
persistent identity: BBa K228236
display id: BBa_K228236
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI->Terminator
creator: Guosheng Zhang
created: 2009-09-19T11:00:00Z
modified: 2015-05-08T01:11:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4413
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We have attached the cI insert to a terminator vector, which made the next step construction more convenient.
mutable notes: The length of terminator part is quite small, so it should be digested to a vector and attached to the cI insert.
mutable provenance: Built by joining different standardized biobricks together
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0051
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K228236_sequence/1

BBa_B0015
type: Component
persistent identity: component2246575
display id: component2246575
version: 1
top level: BBa_K228236
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0051
type: Component
persistent identity: component2246568
display id: component2246568
version: 1
top level: BBa_K228236
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2246568
display id: annotation2246568
version: 1
top level: BBa_K228236
location: https://synbiohub.org/public/igem/BBa_K228236/annotation2246568/range2246568/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K228236/annotation2246568/range2246568/1
type: Range
persistent identity: range2246568
display id: range2246568
version: 1
direction: direction/reverse
top level: BBa_K228236
start: 1
end: 775
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2246575
display id: annotation2246575
version: 1
top level: BBa_K228236
location: https://synbiohub.org/public/igem/BBa_K228236/annotation2246575/range2246575/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K228236/annotation2246575/range2246575/1
type: Range
persistent identity: range2246575
display id: range2246575
version: 1
direction: direction/reverse
top level: BBa_K228236
start: 784
end: 912
orientation: inline

BBa_K592015
type: ComponentDefinition
persistent identity: BBa K592015
display id: BBa_K592015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP Blue Coding Device
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2016-10-14T06:58:34Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _763_
m user id: 31248
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This neatly designed color coding cassette is a prime candidate to replace BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacteria colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized. Promoter is constitutive, the RBS is strong, the double terminator is not B0015 but B0014.
mutable notes: B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times.
mutable provenance: amilCP comes from the genome of Acropora millepora. All other parts are assembled from the iGEM 2011 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592009
component: BBa_J23101
component: BBa_B0034
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_K592009
sequence annotation: BBa_B0034
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K592015_sequence/1

BBa_K592009
type: Component
persistent identity: component2222898
display id: component2222898
version: 1
top level: BBa_K592015
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_J23101
type: Component
persistent identity: component2222894
display id: component2222894
version: 1
top level: BBa_K592015
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_B0034
type: Component
persistent identity: component2222896
display id: component2222896
version: 1
top level: BBa_K592015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0014
type: Component
persistent identity: component2222905
display id: component2222905
version: 1
top level: BBa_K592015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2222905
display id: annotation2222905
version: 1
top level: BBa_K592015
location: https://synbiohub.org/public/igem/BBa_K592015/annotation2222905/range2222905/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K592015/annotation2222905/range2222905/1
type: Range
persistent identity: range2222905
display id: range2222905
version: 1
direction: direction/reverse
top level: BBa_K592015
start: 739
end: 833
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2222898
display id: annotation2222898
version: 1
top level: BBa_K592015
location: https://synbiohub.org/public/igem/BBa_K592015/annotation2222898/range2222898/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K592015/annotation2222898/range2222898/1
type: Range
persistent identity: range2222898
display id: range2222898
version: 1
direction: direction/reverse
top level: BBa_K592015
start: 62
end: 730
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222896
display id: annotation2222896
version: 1
top level: BBa_K592015
location: https://synbiohub.org/public/igem/BBa_K592015/annotation2222896/range2222896/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592015/annotation2222896/range2222896/1
type: Range
persistent identity: range2222896
display id: range2222896
version: 1
direction: direction/reverse
top level: BBa_K592015
start: 44
end: 55
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2222894
display id: annotation2222894
version: 1
top level: BBa_K592015
location: https://synbiohub.org/public/igem/BBa_K592015/annotation2222894/range2222894/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K592015/annotation2222894/range2222894/1
type: Range
persistent identity: range2222894
display id: range2222894
version: 1
direction: direction/reverse
top level: BBa_K592015
start: 1
end: 35
orientation: inline

BBa_K255000
type: ComponentDefinition
persistent identity: BBa K255000
display id: BBa_K255000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: laci-cfp fusion
creator: Navneet Rai
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
group u list: _355_
m user id: 0
owner id: 2363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: this part is laci-cfp fusion protein. laci sequence was obtained from pubmed and was synthesized.
mutable notes: we followed up all the parameters needed to make it as a registry part.
mutable provenance: DNA-synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K255000_sequence/1

BBa_K566023
type: ComponentDefinition
persistent identity: BBa K566023
display id: BBa_K566023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcyA gene (constitutive expression)
creator: Daniel Rodriguez
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pcyA gene transcribed from constitutive promoter.
mutable notes: For the project that this part was made, it was needed to use other Assambly Standard.
mutable provenance: CDS was extracted by PCR, promoter and terminator were Classic BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K566021
component: BBa_K081005
sequence annotation: BBa_K566021
sequence annotation: BBa_B0015
sequence annotation: BBa_K081005
sequence: https://synbiohub.org/public/igem/BBa_K566023_sequence/1

BBa_B0015
type: Component
persistent identity: component2142757
display id: component2142757
version: 1
top level: BBa_K566023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K566021
type: Component
persistent identity: component2142750
display id: component2142750
version: 1
top level: BBa_K566023
access: public
definition: https://synbiohub.org/public/igem/BBa_K566021/1

BBa_K081005
type: Component
persistent identity: component2142748
display id: component2142748
version: 1
top level: BBa_K566023
access: public
definition: https://synbiohub.org/public/igem/BBa_K081005/1

BBa_K566021
type: SequenceAnnotation
persistent identity: annotation2142750
display id: annotation2142750
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142750/range2142750/1
component: BBa_K566021

https://synbiohub.org/public/igem/BBa_K566023/annotation2142750/range2142750/1
type: Range
persistent identity: range2142750
display id: range2142750
version: 1
direction: direction/reverse
top level: BBa_K566023
start: 65
end: 811
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2142757
display id: annotation2142757
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142757/range2142757/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K566023/annotation2142757/range2142757/1
type: Range
persistent identity: range2142757
display id: range2142757
version: 1
direction: direction/reverse
top level: BBa_K566023
start: 820
end: 948
orientation: inline

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2142748
display id: annotation2142748
version: 1
top level: BBa_K566023
location: https://synbiohub.org/public/igem/BBa_K566023/annotation2142748/range2142748/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K566023/annotation2142748/range2142748/1
type: Range
persistent identity: range2142748
display id: range2142748
version: 1
direction: direction/reverse
top level: BBa_K566023
start: 1
end: 58
orientation: inline

BBa_K342003
type: ComponentDefinition
persistent identity: BBa K342003
display id: BBa_K342003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR234 protein, with higher effect on Curli promoter
creator: Nathalie Isorce
created: 2010-10-23T11:00:00Z
modified: 2016-02-12T09:01:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _471_
m user id: 4206
owner id: 6842
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is coding for a mutated protein OmpR. This protein will be phosphorilated by the complex Envz. The phosphorilated protein is an activator of the Curli promoter
mutable notes: enter
mutable provenance: pcr
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K342003_sequence/1

BBa_J04051
type: ComponentDefinition
persistent identity: BBa J04051
display id: BBa_J04051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP with tag
creator: tammy674
created: 2005-06-07T11:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_E1010 with an LVA tag added via PCR.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Start
sequence annotation: mrfp1
sequence annotation: Stop
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J04051_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation1508160
display id: annotation1508160
version: 1
top level: BBa_J04051
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J04051/annotation1508160/range1508160/1

https://synbiohub.org/public/igem/BBa_J04051/annotation1508160/range1508160/1
type: Range
persistent identity: range1508160
display id: range1508160
version: 1
direction: direction/forward
top level: BBa_J04051
start: 1
end: 3
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1507347
display id: annotation1507347
version: 1
top level: BBa_J04051
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J04051/annotation1507347/range1507347/1

https://synbiohub.org/public/igem/BBa_J04051/annotation1507347/range1507347/1
type: Range
persistent identity: range1507347
display id: range1507347
version: 1
direction: direction/forward
top level: BBa_J04051
start: 1
end: 675
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation1507863
display id: annotation1507863
version: 1
top level: BBa_J04051
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J04051/annotation1507863/range1507863/1

https://synbiohub.org/public/igem/BBa_J04051/annotation1507863/range1507863/1
type: Range
persistent identity: range1507863
display id: range1507863
version: 1
direction: direction/forward
top level: BBa_J04051
start: 715
end: 720
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1507968
display id: annotation1507968
version: 1
top level: BBa_J04051
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J04051/annotation1507968/range1507968/1

https://synbiohub.org/public/igem/BBa_J04051/annotation1507968/range1507968/1
type: Range
persistent identity: range1507968
display id: range1507968
version: 1
direction: direction/forward
top level: BBa_J04051
start: 676
end: 714
orientation: inline

BBa_K831009
type: ComponentDefinition
persistent identity: BBa K831009
display id: BBa_K831009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible HipB antitoxin under the control of lac promoter
creator: Silvia J Ca??as
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1090_
m user id: 0
owner id: 9809
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: HipB is the antitoxin of the HipAB toxin-antitoxin (TA) module. HipB neutralizes the toxin effect of HipA due to protein-protein interactions.
mutable notes: None
mutable provenance: This part was extracted of the genome of Escherichia coli K12 MG1655 using high fidelity PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K831001
component: BBa_R0010
sequence annotation: BBa_K831001
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K831009_sequence/1

BBa_K831001
type: Component
persistent identity: component2199424
display id: component2199424
version: 1
top level: BBa_K831009
access: public
definition: https://synbiohub.org/public/igem/BBa_K831001/1

BBa_R0010
type: Component
persistent identity: component2199416
display id: component2199416
version: 1
top level: BBa_K831009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K831001
type: SequenceAnnotation
persistent identity: annotation2199424
display id: annotation2199424
version: 1
top level: BBa_K831009
location: https://synbiohub.org/public/igem/BBa_K831009/annotation2199424/range2199424/1
component: BBa_K831001

https://synbiohub.org/public/igem/BBa_K831009/annotation2199424/range2199424/1
type: Range
persistent identity: range2199424
display id: range2199424
version: 1
direction: direction/reverse
top level: BBa_K831009
start: 207
end: 473
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2199416
display id: annotation2199416
version: 1
top level: BBa_K831009
location: https://synbiohub.org/public/igem/BBa_K831009/annotation2199416/range2199416/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K831009/annotation2199416/range2199416/1
type: Range
persistent identity: range2199416
display id: range2199416
version: 1
direction: direction/forward
top level: BBa_K831009
start: 1
end: 200
orientation: inline

BBa_K123002
type: ComponentDefinition
persistent identity: BBa K123002
display id: BBa_K123002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacIQ ERE TetR
creator: Jason Gardiner
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase.
mutable notes: There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.
mutable provenance: The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: barcode
sequence annotation: tetR
sequence annotation: BBa_C0040
sequence annotation: ERE
sequence annotation: P(Lac) IQ
sequence annotation: SsrA
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_I14032
sequence: https://synbiohub.org/public/igem/BBa_K123002_sequence/1

barcode
type: SequenceAnnotation
persistent identity: annotation1992724
display id: annotation1992724
version: 1
top level: BBa_K123002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992724/range1992724/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992724/range1992724/1
type: Range
persistent identity: range1992724
display id: range1992724
version: 1
direction: direction/reverse
top level: BBa_K123002
start: 704
end: 728
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation1992721
display id: annotation1992721
version: 1
top level: BBa_K123002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992721/range1992721/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992721/range1992721/1
type: Range
persistent identity: range1992721
display id: range1992721
version: 1
direction: direction/forward
top level: BBa_K123002
start: 47
end: 663
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1992723
display id: annotation1992723
version: 1
top level: BBa_K123002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992723/range1992723/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992723/range1992723/1
type: Range
persistent identity: range1992723
display id: range1992723
version: 1
direction: direction/reverse
top level: BBa_K123002
start: 44
end: 703
orientation: inline

ERE
type: SequenceAnnotation
persistent identity: annotation1992733
display id: annotation1992733
version: 1
top level: BBa_K123002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992733/range1992733/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992733/range1992733/1
type: Range
persistent identity: range1992733
display id: range1992733
version: 1
direction: direction/forward
top level: BBa_K123002
start: 43
end: 57
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1992717
display id: annotation1992717
version: 1
top level: BBa_K123002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992717/range1992717/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992717/range1992717/1
type: Range
persistent identity: range1992717
display id: range1992717
version: 1
direction: direction/forward
top level: BBa_K123002
start: 1
end: 37
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1992722
display id: annotation1992722
version: 1
top level: BBa_K123002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992722/range1992722/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992722/range1992722/1
type: Range
persistent identity: range1992722
display id: range1992722
version: 1
direction: direction/forward
top level: BBa_K123002
start: 664
end: 697
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1992719
display id: annotation1992719
version: 1
top level: BBa_K123002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992719/range1992719/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992719/range1992719/1
type: Range
persistent identity: range1992719
display id: range1992719
version: 1
direction: direction/forward
top level: BBa_K123002
start: 26
end: 31
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1992718
display id: annotation1992718
version: 1
top level: BBa_K123002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992718/range1992718/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992718/range1992718/1
type: Range
persistent identity: range1992718
display id: range1992718
version: 1
direction: direction/forward
top level: BBa_K123002
start: 3
end: 8
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992720
display id: annotation1992720
version: 1
top level: BBa_K123002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992720/range1992720/1

https://synbiohub.org/public/igem/BBa_K123002/annotation1992720/range1992720/1
type: Range
persistent identity: range1992720
display id: range1992720
version: 1
direction: direction/reverse
top level: BBa_K123002
start: 1
end: 37
orientation: inline

BBa_K156009
type: ComponentDefinition
persistent identity: BBa K156009
display id: BBa_K156009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OFP (orange fluorescent protein)
creator: George McArthur IV
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter.
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: OFP was cloned from the tentacles of Cerianthus sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156009_sequence/1

BBa_K274382
type: ComponentDefinition
persistent identity: BBa K274382
display id: BBa_K274382
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> PSP3 pag - PP promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746362
component: BBa_B0012
component: BBa_I746351
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746351
sequence annotation: BBa_B0010
sequence annotation: BBa_I746362
sequence: https://synbiohub.org/public/igem/BBa_K274382_sequence/1

BBa_I746362
type: Component
persistent identity: component2055362
display id: component2055362
version: 1
top level: BBa_K274382
access: public
definition: https://synbiohub.org/public/igem/BBa_I746362/1

BBa_B0012
type: Component
persistent identity: component2055357
display id: component2055357
version: 1
top level: BBa_K274382
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746351
type: Component
persistent identity: component2055354
display id: component2055354
version: 1
top level: BBa_K274382
access: public
definition: https://synbiohub.org/public/igem/BBa_I746351/1

BBa_B0010
type: Component
persistent identity: component2055355
display id: component2055355
version: 1
top level: BBa_K274382
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055357
display id: annotation2055357
version: 1
top level: BBa_K274382
location: https://synbiohub.org/public/igem/BBa_K274382/annotation2055357/range2055357/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274382/annotation2055357/range2055357/1
type: Range
persistent identity: range2055357
display id: range2055357
version: 1
direction: direction/forward
top level: BBa_K274382
start: 334
end: 374
orientation: inline

BBa_I746351
type: SequenceAnnotation
persistent identity: annotation2055354
display id: annotation2055354
version: 1
top level: BBa_K274382
location: https://synbiohub.org/public/igem/BBa_K274382/annotation2055354/range2055354/1
component: BBa_I746351

https://synbiohub.org/public/igem/BBa_K274382/annotation2055354/range2055354/1
type: Range
persistent identity: range2055354
display id: range2055354
version: 1
direction: direction/reverse
top level: BBa_K274382
start: 1
end: 237
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055355
display id: annotation2055355
version: 1
top level: BBa_K274382
location: https://synbiohub.org/public/igem/BBa_K274382/annotation2055355/range2055355/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274382/annotation2055355/range2055355/1
type: Range
persistent identity: range2055355
display id: range2055355
version: 1
direction: direction/forward
top level: BBa_K274382
start: 246
end: 325
orientation: inline

BBa_I746362
type: SequenceAnnotation
persistent identity: annotation2055362
display id: annotation2055362
version: 1
top level: BBa_K274382
location: https://synbiohub.org/public/igem/BBa_K274382/annotation2055362/range2055362/1
component: BBa_I746362

https://synbiohub.org/public/igem/BBa_K274382/annotation2055362/range2055362/1
type: Range
persistent identity: range2055362
display id: range2055362
version: 1
direction: direction/reverse
top level: BBa_K274382
start: 383
end: 474
orientation: inline

BBa_K801100
type: ComponentDefinition
persistent identity: BBa K801100
display id: BBa_K801100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device - RFC10 and RFC25 compatible
creator: TU Munich
created: 2012-10-20T11:00:00Z
modified: 2016-10-14T06:54:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 31248
owner id: 14732
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Promoter (lacI regulated) BBa_R0010
sequence annotation: Double Terminator BBa_B0015
sequence annotation: RBS (Elowitz 1999) BBa_B0034
sequence annotation: Barcode
sequence annotation: Red Fluorescent Protein from Discosoma striata BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K801100_sequence/1

Promoter (lacI regulated) BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2213050
display id: annotation2213050
version: 1
top level: BBa_K801100
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213050/range2213050/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213050/range2213050/1
type: Range
persistent identity: range2213050
display id: range2213050
version: 1
direction: direction/forward
top level: BBa_K801100
start: 1
end: 200
orientation: inline

Double Terminator BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2213054
display id: annotation2213054
version: 1
top level: BBa_K801100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213054/range2213054/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213054/range2213054/1
type: Range
persistent identity: range2213054
display id: range2213054
version: 1
direction: direction/forward
top level: BBa_K801100
start: 941
end: 1069
orientation: inline

RBS (Elowitz 1999) BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2213051
display id: annotation2213051
version: 1
top level: BBa_K801100
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213051/range2213051/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213051/range2213051/1
type: Range
persistent identity: range2213051
display id: range2213051
version: 1
direction: direction/forward
top level: BBa_K801100
start: 209
end: 220
orientation: inline

Barcode
type: SequenceAnnotation
persistent identity: annotation2213053
display id: annotation2213053
version: 1
top level: BBa_K801100
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213053/range2213053/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213053/range2213053/1
type: Range
persistent identity: range2213053
display id: range2213053
version: 1
direction: direction/forward
top level: BBa_K801100
start: 908
end: 932
orientation: inline

Red Fluorescent Protein from Discosoma striata BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2213052
display id: annotation2213052
version: 1
top level: BBa_K801100
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213052/range2213052/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213052/range2213052/1
type: Range
persistent identity: range2213052
display id: range2213052
version: 1
direction: direction/forward
top level: BBa_K801100
start: 227
end: 902
orientation: inline

BBa_K1100006
type: ComponentDefinition
persistent identity: BBa K1100006
display id: BBa_K1100006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized sfGFP with Csy4 insulator loci
creator: Hao-tian Guo
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1410_
m user id: 0
owner id: 14489
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Csy4 is a member of CRISPR pathway discovered in Pseudomonas aeruginosa. It is a single endoRNase that recognizes and cleaves a 28-nucleotide repetitive sequence and produces stable transcripts with a 5???hydroxylgroup, which can eliminate unwanted interactions between 5???UTRs and translational elements such as RBSs to standardize the expression of the elements.
mutable notes: Csy4 is a protein used to cleavage the mRNA so that the influence from UTR will be eliminated
mutable provenance: The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: insulator: Csy4 cleavage loci
sequence annotation: sfGFP
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1100006_sequence/1

insulator: Csy4 cleavage loci
type: SequenceAnnotation
persistent identity: annotation2339478
display id: annotation2339478
version: 1
top level: BBa_K1100006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1100006/annotation2339478/range2339478/1

https://synbiohub.org/public/igem/BBa_K1100006/annotation2339478/range2339478/1
type: Range
persistent identity: range2339478
display id: range2339478
version: 1
direction: direction/forward
top level: BBa_K1100006
start: 7
end: 22
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2339688
display id: annotation2339688
version: 1
top level: BBa_K1100006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1100006/annotation2339688/range2339688/1

https://synbiohub.org/public/igem/BBa_K1100006/annotation2339688/range2339688/1
type: Range
persistent identity: range2339688
display id: range2339688
version: 1
direction: direction/forward
top level: BBa_K1100006
start: 41
end: 739
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2339687
display id: annotation2339687
version: 1
top level: BBa_K1100006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1100006/annotation2339687/range2339687/1

https://synbiohub.org/public/igem/BBa_K1100006/annotation2339687/range2339687/1
type: Range
persistent identity: range2339687
display id: range2339687
version: 1
direction: direction/forward
top level: BBa_K1100006
start: 22
end: 34
orientation: inline

BBa_K360121
type: ComponentDefinition
persistent identity: BBa K360121
display id: BBa_K360121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + LovTAP Photosensor + terminator
creator: Claudia Ivonne Hernandez Armenta
created: 2010-09-08T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _485_
m user id: 0
owner id: 6618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This photoreceptor was assembled by Strickland et al., and consists of a LOV (light-oxygen-voltage) domain of Avena sativa phototropin1 (AsLOV2) that senses blue light, fused to the trpR- DNA binding domain of the transcription factor trp repressor. The resulting protein is named LovTAP: LOV- and tryptophan-activated protein. LOV domains bind a flavin-mononucleotide (FMN) or flavin-adenine-dinucleotide (FAD) cofactor, which are used in a wide variety of metabolic pathways as cofactors in redox reactions and are available in most organisms. The cofactor has a broad absorption spectrum, with a maximum at 450 nm. Besides, the core of the LOV domain is often flanked by amino- or carboxy-terminal helices, termed A???α and Jα, respectively In the LovTAP construction, AsLOV2 domain via its carboxyl-terminal Jα ???helix was ligated to an amino-terminal truncation of TrpR. The resulting protein has a domain-domain overlap with a shared helix. Thus, photoexcitation would change the conformation of the protein, in turn changing the stability of the shared-helix-domain contacts. Under the presence of light, absorption of a photon leads to the formation of a covalent adduct between the flavin mononucleotide (FMN) cofactor and a conserved cysteine residue in the AsLOV2 domain, which results in conformational rearrangements in LovTAP. This change impacts the affinity of the shared helix for the two domains: disrupting the contacts between the shared helix and the LOV domain and enabling the association of the shared helix with the TrpR domain, which establishes DNA-binding affinity and LovTAP can then bind DNA as an homodimer, repressing the transcription of the genes downstream of its binding sites. In the dark, when the shared helix contacts the LOV domain, the TrpR domain's DNA-binding affinity decreases and LovTAP is in an inactive conformation.
mutable notes: We decided to synthesize a new LovTAP part, that in comparison with the Part:BBa_K191003 that is already at the registry, has the following differences: 1. The 2 PstI restriction sites were removed from the coding region of LovTap. 2. We included a punctual mutation to change the ILE427 by a PHE427, as was proposed by the model results of the team iGEM09_EPF-Lausanne. With this mutation LovTAP should react faster and the conformational change should be more stable (the protein stays in the active form for longer, under light induction). 3. The part does not include a promoter. We eliminated the inverting regulator sensitive to LacI and CAP protein(Part:BBa_R0010). According to the report of EPF-Laussane team, the expression levels of LovTAP under the inverting regulator, doesn??t seem to show differences to the induction with IPTG. Thus, we decided to remove it and as we know that the level expression of LovTAP must be low (because at high levels of expresion, there is unspecific regulation under darkness and light states), we plan to test weak promoters to choose the best option and include it in the construction. 4. The RBS was changed from a strong (Part: BBa_B0030) to a medium strength (Part:BBa_B0032), thus expecting lower levels of lovTAP protein. 5. The stop codon tga was changed for two taa.
mutable provenance: Chiameric protein assembled by Strickland et al. Reference: Strickland, D., Moffat, K., & Sosnick, T. (2008). Light-activated DNA binding in a designed allosteric protein. Proceedings of the National Academy of Sciences, 105(31), 10709. National Acad Sciences. Retrieved from http://www.pnas.org/content/105/31/10709.full.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: AAA
sequence annotation: stop codon
sequence annotation: RBS B0032
sequence annotation: Terminator B0010
sequence annotation: Terminator B0012
sequence annotation: LovTAP K360022
sequence: https://synbiohub.org/public/igem/BBa_K360121_sequence/1

AAA
type: SequenceAnnotation
persistent identity: annotation2091651
display id: annotation2091651
version: 1
top level: BBa_K360121
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091651/range2091651/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091651/range2091651/1
type: Range
persistent identity: range2091651
display id: range2091651
version: 1
direction: direction/forward
top level: BBa_K360121
start: 831
end: 844
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2091647
display id: annotation2091647
version: 1
top level: BBa_K360121
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091647/range2091647/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091647/range2091647/1
type: Range
persistent identity: range2091647
display id: range2091647
version: 1
direction: direction/forward
top level: BBa_K360121
start: 702
end: 706
orientation: inline

RBS B0032
type: SequenceAnnotation
persistent identity: annotation2091646
display id: annotation2091646
version: 1
top level: BBa_K360121
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091646/range2091646/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091646/range2091646/1
type: Range
persistent identity: range2091646
display id: range2091646
version: 1
direction: direction/forward
top level: BBa_K360121
start: 1
end: 13
orientation: inline

Terminator B0010
type: SequenceAnnotation
persistent identity: annotation2091649
display id: annotation2091649
version: 1
top level: BBa_K360121
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091649/range2091649/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091649/range2091649/1
type: Range
persistent identity: range2091649
display id: range2091649
version: 1
direction: direction/forward
top level: BBa_K360121
start: 725
end: 771
orientation: inline

Terminator B0012
type: SequenceAnnotation
persistent identity: annotation2091650
display id: annotation2091650
version: 1
top level: BBa_K360121
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091650/range2091650/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091650/range2091650/1
type: Range
persistent identity: range2091650
display id: range2091650
version: 1
direction: direction/forward
top level: BBa_K360121
start: 804
end: 830
orientation: inline

LovTAP K360022
type: SequenceAnnotation
persistent identity: annotation2091648
display id: annotation2091648
version: 1
top level: BBa_K360121
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091648/range2091648/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091648/range2091648/1
type: Range
persistent identity: range2091648
display id: range2091648
version: 1
direction: direction/forward
top level: BBa_K360121
start: 18
end: 701
orientation: inline

recAp-cheZ
type: ComponentDefinition
persistent identity: BBa K629010
display id: BBa_K629010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: recAp-cheZ-eGFP
creator: Zilong WANG, Yi ZHENG
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: recA-cheZ-eGFP
mutable notes: none
mutable provenance: TGCGTATGCATTGCAGACCTTGTGGCAACAATTTCTACAAAACACTTGATACTGTATGAGCATACAGTATAATTGCTTCAACAGAACATATTGACTATCCGGTATTACCCGGCATGACAGGAGTAAAAATGGCTATCATGATGCAACCATCAATCAAACCTGCTGACGAGCATTCAGCTGGCGATATCATTGCGCGCATCGGCAGCCTGACGCGTATGCTGCGCGACAGTTTGCGGGAACTGGGGCTGGATCAGGCCATTGCCGAAGCGGCGGAAGCCATCCCCGATGCGCGCGATCGTTTGTACTATGTTGTGCAGATGACCGCCCAGGCTGCGGAGCGGGCGCTGAACAGTGTTGAGGCGTCACAACCGCATCAGGATCAAATGGAGAAATCAGCAAAAGCGTTAACCCAACGTTGGGATGACTGGTTTGCCGATCCGATTGACCTTGCCGACGCCCGTGAACTGGTAACAGATACACGACAATTTCTGGCAGATGTACCCGCGCATACCAGCTTTACTAACGCGCAACTGCTGGAAATCATGATGGCGCAGGATTTTCAGGATCTCACCGGGCAGGTCATTAAGCGGATGATGGATGTCATTCAGGAGATCGAACGCCAGTTGCTGATGGTGCTGTTGGAAAACATCCCGGAACAGGAGTCGCGTCCAAAACGTGAAAACCAGAGTTTGCTTAATGGACCTCAGGTCGATACCAGCAAAGCCGGTGTGGTAGCCAGTCAGGATCAGGTGGACGATTTGTTGGATAGTCTTGGATTTATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K629004
component: BBa_K629001
component: BBa_K629007
sequence annotation: BBa_K629004
sequence annotation: BBa_K629007
sequence annotation: BBa_K629001
sequence: https://synbiohub.org/public/igem/BBa_K629010_sequence/1

BBa_K629004
type: Component
persistent identity: component2152242
display id: component2152242
version: 1
top level: recAp-cheZ
access: public
definition: https://synbiohub.org/public/igem/BBa_K629004/1

BBa_K629001
type: Component
persistent identity: component2152241
display id: component2152241
version: 1
top level: recAp-cheZ
access: public
definition: https://synbiohub.org/public/igem/BBa_K629001/1

BBa_K629007
type: Component
persistent identity: component2152247
display id: component2152247
version: 1
top level: recAp-cheZ
access: public
definition: https://synbiohub.org/public/igem/BBa_K629007/1

BBa_K629004
type: SequenceAnnotation
persistent identity: annotation2152242
display id: annotation2152242
version: 1
top level: recAp-cheZ
location: https://synbiohub.org/public/igem/BBa_K629010/annotation2152242/range2152242/1
component: BBa_K629004

https://synbiohub.org/public/igem/BBa_K629010/annotation2152242/range2152242/1
type: Range
persistent identity: range2152242
display id: range2152242
version: 1
direction: direction/reverse
top level: recAp-cheZ
start: 125
end: 766
orientation: inline

BBa_K629007
type: SequenceAnnotation
persistent identity: annotation2152247
display id: annotation2152247
version: 1
top level: recAp-cheZ
location: https://synbiohub.org/public/igem/BBa_K629010/annotation2152247/range2152247/1
component: BBa_K629007

https://synbiohub.org/public/igem/BBa_K629010/annotation2152247/range2152247/1
type: Range
persistent identity: range2152247
display id: range2152247
version: 1
direction: direction/reverse
top level: recAp-cheZ
start: 767
end: 1486
orientation: inline

BBa_K629001
type: SequenceAnnotation
persistent identity: annotation2152241
display id: annotation2152241
version: 1
top level: recAp-cheZ
location: https://synbiohub.org/public/igem/BBa_K629010/annotation2152241/range2152241/1
component: BBa_K629001

https://synbiohub.org/public/igem/BBa_K629010/annotation2152241/range2152241/1
type: Range
persistent identity: range2152241
display id: range2152241
version: 1
direction: direction/reverse
top level: recAp-cheZ
start: 1
end: 124
orientation: inline

BBa_K1051053
type: ComponentDefinition
persistent identity: BBa K1051053
display id: BBa_K1051053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator)
creator: Jinchun Zhang
created: 2013-07-22T11:00:00Z
modified: 2015-05-08T01:08:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 15923
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Yeast terminator TBY-1 joint with red flourescent protein. After added with 23 suffix and preffix, this composite biobricks can be joint with other coding gene sequence in our project. Thus it would be a reporter showing red flourescent,also as a terminator which can stop the transcription.
mutable notes: No
mutable provenance: Saccharomyces cerevisiae genomics sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1051006
component: BBa_K1051001
sequence annotation: BBa_K1051001
sequence annotation: BBa_K1051006
sequence: https://synbiohub.org/public/igem/BBa_K1051053_sequence/1

BBa_K1051006
type: Component
persistent identity: component2331041
display id: component2331041
version: 1
top level: BBa_K1051053
access: public
definition: https://synbiohub.org/public/igem/BBa_K1051006/1

BBa_K1051001
type: Component
persistent identity: component2331040
display id: component2331040
version: 1
top level: BBa_K1051053
access: public
definition: BBa_K1051001

BBa_K1051001
type: SequenceAnnotation
persistent identity: annotation2331040
display id: annotation2331040
version: 1
top level: BBa_K1051053
location: https://synbiohub.org/public/igem/BBa_K1051053/annotation2331040/range2331040/1
component: BBa_K1051001

https://synbiohub.org/public/igem/BBa_K1051053/annotation2331040/range2331040/1
type: Range
persistent identity: range2331040
display id: range2331040
version: 1
direction: direction/reverse
top level: BBa_K1051053
start: 1
end: 716
orientation: inline

BBa_K1051006
type: SequenceAnnotation
persistent identity: annotation2331041
display id: annotation2331041
version: 1
top level: BBa_K1051053
location: https://synbiohub.org/public/igem/BBa_K1051053/annotation2331041/range2331041/1
component: BBa_K1051006

https://synbiohub.org/public/igem/BBa_K1051053/annotation2331041/range2331041/1
type: Range
persistent identity: range2331041
display id: range2331041
version: 1
direction: direction/reverse
top level: BBa_K1051053
start: 725
end: 755
orientation: inline

BBa_K182005
type: ComponentDefinition
persistent identity: BBa K182005
display id: BBa_K182005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR regulated by CI operator (RBS+, Term-) with LVA tag
creator: MD. RISAT UL HAQUE, Daniel J. Leirer
created: 2009-08-12T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _279_
m user id: 0
owner id: 3989
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a part using the biobrick BBa_K182004 as the upstream part and BBa_S03518 as the downstream part. BBa_K182004 is a cI regulated promoter. The promoter has two binding sites for the cI repressor and is repressed when it binds.It is identical to BBa_R0051 apart from a point mutation in the -35 promoter binding region(15th base is C instead of T). BBa_S03518 is a TetR protein with a Ribosome Binding site upstream and an LVA tag attached to its end. TetR binds to TetR regulated promoters such as BBa_R0040. aTc (anhydrotetracycline) binds to TetR causing it to be released from the promoter and reintroducing transcription. This part comes without a terminator.
mutable notes: BBa_K182004 was used as an upstream part and BBa_S03518 as a downstream part.
mutable provenance: BBa_K182004 , BBa_S03518
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_S03518
component: BBa_K182004
sequence annotation: BBa_S03518
sequence annotation: BBa_K182004
sequence: https://synbiohub.org/public/igem/BBa_K182005_sequence/1

BBa_S03518
type: Component
persistent identity: component2246499
display id: component2246499
version: 1
top level: BBa_K182005
access: public
definition: https://synbiohub.org/public/igem/BBa_S03518/1

BBa_K182004
type: Component
persistent identity: component2246487
display id: component2246487
version: 1
top level: BBa_K182005
access: public
definition: https://synbiohub.org/public/igem/BBa_K182004/1

BBa_S03518
type: SequenceAnnotation
persistent identity: annotation2246499
display id: annotation2246499
version: 1
top level: BBa_K182005
location: https://synbiohub.org/public/igem/BBa_K182005/annotation2246499/range2246499/1
component: BBa_S03518

https://synbiohub.org/public/igem/BBa_K182005/annotation2246499/range2246499/1
type: Range
persistent identity: range2246499
display id: range2246499
version: 1
direction: direction/reverse
top level: BBa_K182005
start: 58
end: 760
orientation: inline

BBa_K182004
type: SequenceAnnotation
persistent identity: annotation2246487
display id: annotation2246487
version: 1
top level: BBa_K182005
location: https://synbiohub.org/public/igem/BBa_K182005/annotation2246487/range2246487/1
component: BBa_K182004

https://synbiohub.org/public/igem/BBa_K182005/annotation2246487/range2246487/1
type: Range
persistent identity: range2246487
display id: range2246487
version: 1
direction: direction/forward
top level: BBa_K182005
start: 1
end: 49
orientation: inline

BBa_K133016
type: ComponentDefinition
persistent identity: BBa K133016
display id: BBa_K133016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CF
creator: Vid Kocar
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3351
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Whole chimeric protein
mutable notes: None
mutable provenance: Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133016_sequence/1

AmCyan
type: ComponentDefinition
persistent identity: BBa J176022
display id: BBa_J176022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AmCyan
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: cyan fluorescent protein from PamCyan-1C1
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176022_sequence/1

Lac
type: ComponentDefinition
persistent identity: BBa K500003
display id: BBa_K500003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lignin degradation 4
creator: Bin Jia
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _596_
m user id: 0
owner id: 4808
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: substrate
mutable notes: no
mutable provenance: trametes veorsicolor
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Glox
sequence annotation: PRO
sequence: https://synbiohub.org/public/igem/BBa_K500003_sequence/1

Glox
type: SequenceAnnotation
persistent identity: annotation2109129
display id: annotation2109129
version: 1
top level: Lac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K500003/annotation2109129/range2109129/1

https://synbiohub.org/public/igem/BBa_K500003/annotation2109129/range2109129/1
type: Range
persistent identity: range2109129
display id: range2109129
version: 1
direction: direction/forward
top level: Lac
start: 4
end: 1677
orientation: inline

PRO
type: SequenceAnnotation
persistent identity: annotation2104211
display id: annotation2104211
version: 1
top level: Lac
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K500003/annotation2104211/range2104211/1

https://synbiohub.org/public/igem/BBa_K500003/annotation2104211/range2104211/1
type: Range
persistent identity: range2104211
display id: range2104211
version: 1
direction: direction/forward
top level: Lac
start: 1
end: 3
orientation: inline

BBa_K228009
type: ComponentDefinition
persistent identity: BBa K228009
display id: BBa_K228009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC protein(reversed sequence) and Pbad promoter
creator: Gao Rencheng
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.
mutable notes: The maximum concentration of L-arabinose we tested is 10^-4 M.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I13453
component: BBa_K228008
sequence annotation: BBa_I13453
sequence annotation: BBa_K228008
sequence: https://synbiohub.org/public/igem/BBa_K228009_sequence/1

BBa_I13453
type: Component
persistent identity: component2244234
display id: component2244234
version: 1
top level: BBa_K228009
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_I13453
type: Component
persistent identity: component2244232
display id: component2244232
version: 1
top level: BBa_K228009
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K228008
type: Component
persistent identity: component2244233
display id: component2244233
version: 1
top level: BBa_K228009
access: public
definition: https://synbiohub.org/public/igem/BBa_K228008/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244232
display id: annotation2244232
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
type: Range
persistent identity: range2244232
display id: range2244232
version: 1
direction: direction/reverse
top level: BBa_K228009
start: 1150
end: 1279
orientation: inline

BBa_K228008
type: SequenceAnnotation
persistent identity: annotation2244233
display id: annotation2244233
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
component: BBa_K228008

https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
type: Range
persistent identity: range2244233
display id: range2244233
version: 1
direction: direction/reverse
top level: BBa_K228009
start: 1
end: 1141
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244234
display id: annotation2244234
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
type: Range
persistent identity: range2244234
display id: range2244234
version: 1
direction: direction/reverse
top level: BBa_K228009
start: 1150
end: 1279
orientation: inline

BBa_P10306
type: ComponentDefinition
persistent identity: BBa P10306
display id: BBa_P10306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tRFP
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T06:01:27Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP50018
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10306_sequence/1

BBa_I20270
type: ComponentDefinition
persistent identity: BBa I20270
display id: BBa_I20270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter MeasKit (J23151)
creator: Jason Kelly
created: 2008-02-06T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: J23108 inserted in the Promoter MeasKit
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_J23151
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_J23151
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I20270_sequence/1

BBa_E0040
type: Component
persistent identity: component1961204
display id: component1961204
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1961205
display id: component1961205
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1961207
display id: component1961207
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23151
type: Component
persistent identity: component1961199
display id: component1961199
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_J23151/1

BBa_B0032
type: Component
persistent identity: component1961201
display id: component1961201
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961205
display id: annotation1961205
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961205/range1961205/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I20270/annotation1961205/range1961205/1
type: Range
persistent identity: range1961205
display id: range1961205
version: 1
direction: direction/forward
top level: BBa_I20270
start: 791
end: 870
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1961201
display id: annotation1961201
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961201/range1961201/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I20270/annotation1961201/range1961201/1
type: Range
persistent identity: range1961201
display id: range1961201
version: 1
direction: direction/forward
top level: BBa_I20270
start: 44
end: 56
orientation: inline

BBa_J23151
type: SequenceAnnotation
persistent identity: annotation1961199
display id: annotation1961199
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961199/range1961199/1
component: BBa_J23151

https://synbiohub.org/public/igem/BBa_I20270/annotation1961199/range1961199/1
type: Range
persistent identity: range1961199
display id: range1961199
version: 1
direction: direction/reverse
top level: BBa_I20270
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961207
display id: annotation1961207
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961207/range1961207/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I20270/annotation1961207/range1961207/1
type: Range
persistent identity: range1961207
display id: range1961207
version: 1
direction: direction/forward
top level: BBa_I20270
start: 879
end: 919
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1961204
display id: annotation1961204
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961204/range1961204/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I20270/annotation1961204/range1961204/1
type: Range
persistent identity: range1961204
display id: range1961204
version: 1
direction: direction/reverse
top level: BBa_I20270
start: 63
end: 782
orientation: inline

ADC
type: ComponentDefinition
persistent identity: BBa K590031
display id: BBa_K590031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aldehyde Decarbonylase (ADC) - Synechococcus elongatus PCC7942
creator: Lei Zheng
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Produces the enzyme that removes the carbonyl group (C=O) from the fatty aldehyde, yielding an alkane one carbon shorter than the original Acyl-ACP and a molecule of formate
mutable notes: Aldehyde decarbonylase gene from synechococcus elongatus
mutable provenance: Ordered from GenScript
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K590031_sequence/1

BBa_J06803
type: ComponentDefinition
persistent identity: BBa J06803
display id: BBa_J06803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0034.J06503.B0015.R0051)
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lambda cI857 QPI w/ strong RBS
mutable notes: First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutate failed. Sent out to Blue Heron for synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0051
component: BBa_B0010
component: BBa_J06503
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_J06503
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J06803_sequence/1

BBa_R0051
type: Component
persistent identity: component1550040
display id: component1550040
version: 1
top level: BBa_J06803
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0010
type: Component
persistent identity: component1550015
display id: component1550015
version: 1
top level: BBa_J06803
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J06503
type: Component
persistent identity: component1550010
display id: component1550010
version: 1
top level: BBa_J06803
access: public
definition: cI857

BBa_B0012
type: Component
persistent identity: component1550025
display id: component1550025
version: 1
top level: BBa_J06803
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1549996
display id: component1549996
version: 1
top level: BBa_J06803
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1550040
display id: annotation1550040
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1550040/range1550040/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J06803/annotation1550040/range1550040/1
type: Range
persistent identity: range1550040
display id: range1550040
version: 1
direction: direction/forward
top level: BBa_J06803
start: 939
end: 987
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1549996
display id: annotation1549996
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1549996/range1549996/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06803/annotation1549996/range1549996/1
type: Range
persistent identity: range1549996
display id: range1549996
version: 1
direction: direction/forward
top level: BBa_J06803
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1550015
display id: annotation1550015
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1550015/range1550015/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06803/annotation1550015/range1550015/1
type: Range
persistent identity: range1550015
display id: range1550015
version: 1
direction: direction/forward
top level: BBa_J06803
start: 802
end: 881
orientation: inline

BBa_J06503
type: SequenceAnnotation
persistent identity: annotation1550010
display id: annotation1550010
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1550010/range1550010/1
component: BBa_J06503

https://synbiohub.org/public/igem/BBa_J06803/annotation1550010/range1550010/1
type: Range
persistent identity: range1550010
display id: range1550010
version: 1
direction: direction/forward
top level: BBa_J06803
start: 19
end: 793
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1550025
display id: annotation1550025
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1550025/range1550025/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06803/annotation1550025/range1550025/1
type: Range
persistent identity: range1550025
display id: range1550025
version: 1
direction: direction/forward
top level: BBa_J06803
start: 890
end: 930
orientation: inline

BBa_K763003
type: ComponentDefinition
persistent identity: BBa K763003
display id: BBa_K763003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGlnA + Gene encoding ZsYellow1
creator: Pedro Luis Dorado Morales
created: 2012-09-06T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1014_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: When there is no ammonia in the media the RNA-polimerase binds to RpoN Promoter and the transcription starts. Why? In gram-negative bacteria, transcriptional activation in response to some external stimuli (absence of nitrogen, for example) often involves the alternative sigma factor, Sigma 54. This factor, also called RpoN or Sigma N, was originally identified as the sigma factor for nitrogen-controlled genes. Sigma 54 works in conjunction with members of the NtrC (Nitrogen regulatory protein C) superfamily of transcriptional activators. ???RpoN Promoter??? contains a binding site which is recognized by the RNA-polimerase when Sigma 54 is binding to this protein. The polimerase binds to RpoN promoter which is present on genes/operons whose products minimize the slowing of growth under nitrogen-limiting conditions (aprox. 2% of the E. coli genome appears to be under NtrC control).
mutable notes: No design considerations needed.
mutable provenance: The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K763003_sequence/1

BBa_K883001
type: ComponentDefinition
persistent identity: BBa K883001
display id: BBa_K883001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCMV wt coat protein under IPTG induced promoter
creator: Jeroen Bosman
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
group u list: _1146_
m user id: 0
owner id: 12652
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: komt nog
mutable notes: none
mutable provenance: this part is created with a part from the parts registry, and a part made by Wageningen_UR 2012 with the template of Dr. Kormelink
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K883000
component: BBa_J04500
sequence annotation: BBa_K883000
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K883001_sequence/1

BBa_K883000
type: Component
persistent identity: component2194186
display id: component2194186
version: 1
top level: BBa_K883001
access: public
definition: https://synbiohub.org/public/igem/BBa_K883000/1

BBa_J04500
type: Component
persistent identity: component2194182
display id: component2194182
version: 1
top level: BBa_K883001
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K883000
type: SequenceAnnotation
persistent identity: annotation2194186
display id: annotation2194186
version: 1
top level: BBa_K883001
location: https://synbiohub.org/public/igem/BBa_K883001/annotation2194186/range2194186/1
component: BBa_K883000

https://synbiohub.org/public/igem/BBa_K883001/annotation2194186/range2194186/1
type: Range
persistent identity: range2194186
display id: range2194186
version: 1
direction: direction/reverse
top level: BBa_K883001
start: 227
end: 799
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2194182
display id: annotation2194182
version: 1
top level: BBa_K883001
location: https://synbiohub.org/public/igem/BBa_K883001/annotation2194182/range2194182/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K883001/annotation2194182/range2194182/1
type: Range
persistent identity: range2194182
display id: range2194182
version: 1
direction: direction/reverse
top level: BBa_K883001
start: 1
end: 220
orientation: inline

DVR1
type: ComponentDefinition
persistent identity: BBa K1080012
display id: BBa_K1080012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DVR1
creator: Macquarie University
created: 2013-09-21T11:00:00Z
modified: 2015-05-08T01:09:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 0
owner id: 18612
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: THSHERRNBHYDF
mutable notes: STHSHTSHD
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DVR1
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1080012_sequence/1

DVR1
type: SequenceAnnotation
persistent identity: annotation2525688
display id: annotation2525688
version: 1
top level: DVR1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1080012/annotation2525688/range2525688/1

https://synbiohub.org/public/igem/BBa_K1080012/annotation2525688/range2525688/1
type: Range
persistent identity: range2525688
display id: range2525688
version: 1
direction: direction/forward
top level: DVR1
start: 25
end: 1000
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2525687
display id: annotation2525687
version: 1
top level: DVR1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1080012/annotation2525687/range2525687/1

https://synbiohub.org/public/igem/BBa_K1080012/annotation2525687/range2525687/1
type: Range
persistent identity: range2525687
display id: range2525687
version: 1
direction: direction/forward
top level: DVR1
start: 6
end: 16
orientation: inline

BBa_K1051259
type: ComponentDefinition
persistent identity: BBa K1051259
display id: BBa_K1051259
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Construct the measurement pathway of K1051208.
creator: Rui Guan
created: 2013-09-01T11:00:00Z
modified: 2015-05-08T01:08:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1358_
m user id: 0
owner id: 14515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Construct the final pathway with M0052
mutable notes: Construct the final pathway with M0052
mutable provenance: Construct the final pathway with M0052
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J04500
component: BBa_K1051208
component: BBa_K1051000
sequence annotation: BBa_K1051208
sequence annotation: BBa_K1051000
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1051259_sequence/1

BBa_J04500
type: Component
persistent identity: component2362676
display id: component2362676
version: 1
top level: BBa_K1051259
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1051208
type: Component
persistent identity: component2362678
display id: component2362678
version: 1
top level: BBa_K1051259
access: public
definition: https://synbiohub.org/public/igem/BBa_K1051208/1

BBa_K1051000
type: Component
persistent identity: component2362677
display id: component2362677
version: 1
top level: BBa_K1051259
access: public
definition: BBa_K1051000

BBa_K1051208
type: SequenceAnnotation
persistent identity: annotation2362678
display id: annotation2362678
version: 1
top level: BBa_K1051259
location: https://synbiohub.org/public/igem/BBa_K1051259/annotation2362678/range2362678/1
component: BBa_K1051208

https://synbiohub.org/public/igem/BBa_K1051259/annotation2362678/range2362678/1
type: Range
persistent identity: range2362678
display id: range2362678
version: 1
direction: direction/reverse
top level: BBa_K1051259
start: 910
end: 948
orientation: inline

BBa_K1051000
type: SequenceAnnotation
persistent identity: annotation2362677
display id: annotation2362677
version: 1
top level: BBa_K1051259
location: https://synbiohub.org/public/igem/BBa_K1051259/annotation2362677/range2362677/1
component: BBa_K1051000

https://synbiohub.org/public/igem/BBa_K1051259/annotation2362677/range2362677/1
type: Range
persistent identity: range2362677
display id: range2362677
version: 1
direction: direction/reverse
top level: BBa_K1051259
start: 227
end: 901
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2362676
display id: annotation2362676
version: 1
top level: BBa_K1051259
location: https://synbiohub.org/public/igem/BBa_K1051259/annotation2362676/range2362676/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1051259/annotation2362676/range2362676/1
type: Range
persistent identity: range2362676
display id: range2362676
version: 1
direction: direction/reverse
top level: BBa_K1051259
start: 1
end: 220
orientation: inline

BBa_J06701
type: ComponentDefinition
persistent identity: BBa J06701
display id: BBa_J06701
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry with RBS and forward terminator
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Combines BBa_B0034 RBS (Elowitz 1999) with BBa_J06601 mCherry with forward terminator
mutable notes: Test constructs involving this part had no fluorescence observed. Sequencing returned nothing resembling the expected part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_E2060
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E2060
sequence: https://synbiohub.org/public/igem/BBa_J06701_sequence/1

BBa_B0012
type: Component
persistent identity: component1531800
display id: component1531800
version: 1
top level: BBa_J06701
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1531771
display id: component1531771
version: 1
top level: BBa_J06701
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1531790
display id: component1531790
version: 1
top level: BBa_J06701
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E2060
type: Component
persistent identity: component1531784
display id: component1531784
version: 1
top level: BBa_J06701
access: public
definition: https://synbiohub.org/public/igem/BBa_E2060/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1531771
display id: annotation1531771
version: 1
top level: BBa_J06701
location: https://synbiohub.org/public/igem/BBa_J06701/annotation1531771/range1531771/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06701/annotation1531771/range1531771/1
type: Range
persistent identity: range1531771
display id: range1531771
version: 1
direction: direction/forward
top level: BBa_J06701
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1531790
display id: annotation1531790
version: 1
top level: BBa_J06701
location: https://synbiohub.org/public/igem/BBa_J06701/annotation1531790/range1531790/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06701/annotation1531790/range1531790/1
type: Range
persistent identity: range1531790
display id: range1531790
version: 1
direction: direction/forward
top level: BBa_J06701
start: 796
end: 875
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1531800
display id: annotation1531800
version: 1
top level: BBa_J06701
location: https://synbiohub.org/public/igem/BBa_J06701/annotation1531800/range1531800/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06701/annotation1531800/range1531800/1
type: Range
persistent identity: range1531800
display id: range1531800
version: 1
direction: direction/forward
top level: BBa_J06701
start: 884
end: 924
orientation: inline

BBa_E2060
type: SequenceAnnotation
persistent identity: annotation1531784
display id: annotation1531784
version: 1
top level: BBa_J06701
location: https://synbiohub.org/public/igem/BBa_J06701/annotation1531784/range1531784/1
component: BBa_E2060

https://synbiohub.org/public/igem/BBa_J06701/annotation1531784/range1531784/1
type: Range
persistent identity: range1531784
display id: range1531784
version: 1
direction: direction/forward
top level: BBa_J06701
start: 19
end: 762
orientation: inline

cheZ Unit
type: ComponentDefinition
persistent identity: BBa K518007
display id: BBa_K518007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ expression cassette (no promoter)
creator: Masato Ohgishi
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. With a promoter of your interest, this device rescues the mobility of cheZ-/- cells.
mutable notes: None.
mutable provenance: cheZ CDS is cloned from Escherichia coli JM109 strain. Other sequences are from BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0014
component: BBa_K518005
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K518005
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K518007_sequence/1

BBa_B0014
type: Component
persistent identity: component2134268
display id: component2134268
version: 1
top level: cheZ Unit
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K518005
type: Component
persistent identity: component2134261
display id: component2134261
version: 1
top level: cheZ Unit
access: public
definition: BBa_K518005

BBa_B0032
type: Component
persistent identity: component2134259
display id: component2134259
version: 1
top level: cheZ Unit
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2134259
display id: annotation2134259
version: 1
top level: cheZ Unit
location: https://synbiohub.org/public/igem/BBa_K518007/annotation2134259/range2134259/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518007/annotation2134259/range2134259/1
type: Range
persistent identity: range2134259
display id: range2134259
version: 1
direction: direction/forward
top level: cheZ Unit
start: 1
end: 13
orientation: inline

BBa_K518005
type: SequenceAnnotation
persistent identity: annotation2134261
display id: annotation2134261
version: 1
top level: cheZ Unit
location: https://synbiohub.org/public/igem/BBa_K518007/annotation2134261/range2134261/1
component: BBa_K518005

https://synbiohub.org/public/igem/BBa_K518007/annotation2134261/range2134261/1
type: Range
persistent identity: range2134261
display id: range2134261
version: 1
direction: direction/reverse
top level: cheZ Unit
start: 22
end: 826
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2134268
display id: annotation2134268
version: 1
top level: cheZ Unit
location: https://synbiohub.org/public/igem/BBa_K518007/annotation2134268/range2134268/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518007/annotation2134268/range2134268/1
type: Range
persistent identity: range2134268
display id: range2134268
version: 1
direction: direction/reverse
top level: cheZ Unit
start: 835
end: 929
orientation: inline

virG
type: ComponentDefinition
persistent identity: BBa K389002
display id: BBa_K389002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated virG
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
mutable notes: synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: virG
sequence annotation: RBS
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389002_sequence/1

virG
type: SequenceAnnotation
persistent identity: annotation2078361
display id: annotation2078361
version: 1
top level: virG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
type: Range
persistent identity: range2078361
display id: range2078361
version: 1
direction: direction/forward
top level: virG
start: 12
end: 737
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078360
display id: annotation2078360
version: 1
top level: virG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
type: Range
persistent identity: range2078360
display id: range2078360
version: 1
direction: direction/forward
top level: virG
start: 4
end: 8
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078362
display id: annotation2078362
version: 1
top level: virG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
type: Range
persistent identity: range2078362
display id: range2078362
version: 1
direction: direction/forward
top level: virG
start: 738
end: 740
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa K143033
display id: BBa_K143033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI (Lva-, N-terminal deletion) regulatory protein
creator: Chris Hirst
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as IPTG or certain sugars. LacI if the regulator protein for the lactose operon in ''E.coli'' and the hyper-spank protein of ''B. subtilis''#1( BBaK143015 ) and is responsible for ensuring that in the absence of lactose (or IPTG) that there is no expression trough these promoter. LacI is not endogenous to ''B. subtilis'', so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes. This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI ( BBa_C0012</bbpart)> and is derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E.coli'' (in differing strains). LacI was used in conjunction with the '''Hyper-spank promoter''' ( BBa_K143015 ) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output ====References==== #1 pmid=16166525
mutable notes: LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks a Ltva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein ( BBa_K143015 ) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene
mutable provenance: The LacI gene was cloned from''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: LacI (Lva-, N-terminal deletion) regulatory protein
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K143033_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1994272
display id: annotation1994272
version: 1
top level: LacI
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1

https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1
type: Range
persistent identity: range1994272
display id: range1994272
version: 1
direction: direction/forward
top level: LacI
start: 1084
end: 1086
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1994271
display id: annotation1994271
version: 1
top level: LacI
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1

https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1
type: Range
persistent identity: range1994271
display id: range1994271
version: 1
direction: direction/forward
top level: LacI
start: 1081
end: 1083
orientation: inline

LacI (Lva-, N-terminal deletion) regulatory protein
type: SequenceAnnotation
persistent identity: annotation1975974
display id: annotation1975974
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1

https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1
type: Range
persistent identity: range1975974
display id: range1975974
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 1080
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992702
display id: annotation1992702
version: 1
top level: LacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1

https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1
type: Range
persistent identity: range1992702
display id: range1992702
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 3
orientation: inline

BBa_K331025
type: ComponentDefinition
persistent identity: BBa K331025
display id: BBa_K331025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS with C-terminal Oligo Arginine - ECFP Fusion
creator: Adam Smith
created: 2010-09-28T11:00:00Z
modified: 2015-05-08T01:12:04Z
discontinued: false
dominant: false
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a oligo arginine sequence fused to the C-terminus of an enhanced cyan fluorescent protein. This will be a component of the proof-of-concept part BBa_K331019 which will show localization of proteins into the interior of a microcompartment formed by the assemly of lumazine synthase proteins.
mutable notes: We chose not to include a transcriptional promoter to this part because we wanted the freedom to put it under the control of varied promoters. We also chose not to include a transcriptional terminator to this part because we planned to use it in an operon, where we would be expressing multiple proteins from a single transcript.
mutable provenance: This sequence was synthesized by Gene Art
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K331002
sequence annotation: BBa_B0034
sequence annotation: BBa_K331002
sequence: https://synbiohub.org/public/igem/BBa_K331025_sequence/1

BBa_B0034
type: Component
persistent identity: component2081119
display id: component2081119
version: 1
top level: BBa_K331025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K331002
type: Component
persistent identity: component2081123
display id: component2081123
version: 1
top level: BBa_K331025
access: public
definition: https://synbiohub.org/public/igem/BBa_K331002/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2081119
display id: annotation2081119
version: 1
top level: BBa_K331025
location: https://synbiohub.org/public/igem/BBa_K331025/annotation2081119/range2081119/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331025/annotation2081119/range2081119/1
type: Range
persistent identity: range2081119
display id: range2081119
version: 1
direction: direction/reverse
top level: BBa_K331025
start: 1
end: 12
orientation: inline

BBa_K331002
type: SequenceAnnotation
persistent identity: annotation2081123
display id: annotation2081123
version: 1
top level: BBa_K331025
location: https://synbiohub.org/public/igem/BBa_K331025/annotation2081123/range2081123/1
component: BBa_K331002

https://synbiohub.org/public/igem/BBa_K331025/annotation2081123/range2081123/1
type: Range
persistent identity: range2081123
display id: range2081123
version: 1
direction: direction/reverse
top level: BBa_K331025
start: 19
end: 774
orientation: inline

BBa_K283005
type: ComponentDefinition
persistent identity: BBa K283005
display id: BBa_K283005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lpp_ompA_eGFP_streptavidin
creator: Yu bin, Jiang QinQin
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part consists of a fusion protein Lpp-rbs-protein under the pLac promoter. The Lpp is a signal peptide. The ompA domain of the fusion directs the protein to the surface membrane. The pLac promoter can be repressed by LacI protein. This part lacks a terminator.
mutable notes: BBa_K283005
mutable provenance: The pLac promoter, rbs sequence are obtained from the registry The fusion protein coding sequence is self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K283009
component: BBa_J36835
component: BBa_J36837
sequence annotation: BBa_J36837
sequence annotation: BBa_K283009
sequence annotation: BBa_J36835
sequence: https://synbiohub.org/public/igem/BBa_K283005_sequence/1

BBa_K283009
type: Component
persistent identity: component2055627
display id: component2055627
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_K283009/1

BBa_J36835
type: Component
persistent identity: component2055624
display id: component2055624
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_J36835/1

BBa_J36837
type: Component
persistent identity: component2055625
display id: component2055625
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_J36837/1

BBa_J36837
type: SequenceAnnotation
persistent identity: annotation2055625
display id: annotation2055625
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055625/range2055625/1
component: BBa_J36837

https://synbiohub.org/public/igem/BBa_K283005/annotation2055625/range2055625/1
type: Range
persistent identity: range2055625
display id: range2055625
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 88
end: 429
orientation: inline

BBa_K283009
type: SequenceAnnotation
persistent identity: annotation2055627
display id: annotation2055627
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055627/range2055627/1
component: BBa_K283009

https://synbiohub.org/public/igem/BBa_K283005/annotation2055627/range2055627/1
type: Range
persistent identity: range2055627
display id: range2055627
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 430
end: 1533
orientation: inline

BBa_J36835
type: SequenceAnnotation
persistent identity: annotation2055624
display id: annotation2055624
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055624/range2055624/1
component: BBa_J36835

https://synbiohub.org/public/igem/BBa_K283005/annotation2055624/range2055624/1
type: Range
persistent identity: range2055624
display id: range2055624
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 1
end: 87
orientation: inline

BBa_K607040
type: ComponentDefinition
persistent identity: BBa K607040
display id: BBa_K607040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: reverse pBad/araC promoter
creator: Olesja Popow
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
group u list: _779_
m user id: 0
owner id: 9177
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: reversed version of BBa_I0500
mutable notes: none
mutable provenance: distribution 2011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K607040_sequence/1

BBa_P10303
type: ComponentDefinition
persistent identity: BBa P10303
display id: BBa_P10303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:57:59Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP41531
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10303_sequence/1

BBa_I742113
type: ComponentDefinition
persistent identity: BBa I742113
display id: BBa_I742113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ech gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: true
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: encodes enoyl CoA hydratase (cleaves CoA group from feruloyl CoA) ech converts feruloyl CoA to vanillin catalyses the final stage of the vanillin biosynthesis pathway
mutable notes: P. fluorescens has a high GC genome, therefore we had the coding region optimised for expression in E. coli. Gene was synthesised by geneart removed one PstI site
mutable provenance: Pseudomonas fluorescens NCIMB 9046 Gene was synthesised by geneart Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I742112
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_I742112
sequence: https://synbiohub.org/public/igem/BBa_I742113_sequence/1

BBa_I742112
type: Component
persistent identity: component1949791
display id: component1949791
version: 1
top level: BBa_I742113
access: public
definition: https://synbiohub.org/public/igem/BBa_I742112/1

BBa_J15001
type: Component
persistent identity: component1949787
display id: component1949787
version: 1
top level: BBa_I742113
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1949787
display id: annotation1949787
version: 1
top level: BBa_I742113
location: https://synbiohub.org/public/igem/BBa_I742113/annotation1949787/range1949787/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742113/annotation1949787/range1949787/1
type: Range
persistent identity: range1949787
display id: range1949787
version: 1
direction: direction/reverse
top level: BBa_I742113
start: 1
end: 10
orientation: inline

BBa_I742112
type: SequenceAnnotation
persistent identity: annotation1949791
display id: annotation1949791
version: 1
top level: BBa_I742113
location: https://synbiohub.org/public/igem/BBa_I742113/annotation1949791/range1949791/1
component: BBa_I742112

https://synbiohub.org/public/igem/BBa_I742113/annotation1949791/range1949791/1
type: Range
persistent identity: range1949791
display id: range1949791
version: 1
direction: direction/reverse
top level: BBa_I742113
start: 17
end: 850
orientation: inline

BBa_K608016
type: ComponentDefinition
persistent identity: BBa K608016
display id: BBa_K608016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium promoter and strong RBS with RFP
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promotor,RBS with RFP
mutable notes: composite
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E1010
component: BBa_K608005
sequence annotation: BBa_E1010
sequence annotation: BBa_K608005
sequence: https://synbiohub.org/public/igem/BBa_K608016_sequence/1

BBa_E1010
type: Component
persistent identity: component2247914
display id: component2247914
version: 1
top level: BBa_K608016
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K608005
type: Component
persistent identity: component2247911
display id: component2247911
version: 1
top level: BBa_K608016
access: public
definition: https://synbiohub.org/public/igem/BBa_K608005/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247914
display id: annotation2247914
version: 1
top level: BBa_K608016
location: https://synbiohub.org/public/igem/BBa_K608016/annotation2247914/range2247914/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K608016/annotation2247914/range2247914/1
type: Range
persistent identity: range2247914
display id: range2247914
version: 1
direction: direction/reverse
top level: BBa_K608016
start: 62
end: 767
orientation: inline

BBa_K608005
type: SequenceAnnotation
persistent identity: annotation2247911
display id: annotation2247911
version: 1
top level: BBa_K608016
location: https://synbiohub.org/public/igem/BBa_K608016/annotation2247911/range2247911/1
component: BBa_K608005

https://synbiohub.org/public/igem/BBa_K608016/annotation2247911/range2247911/1
type: Range
persistent identity: range2247911
display id: range2247911
version: 1
direction: direction/reverse
top level: BBa_K608016
start: 1
end: 55
orientation: inline

BBa_K222000
type: ComponentDefinition
persistent identity: BBa K222000
display id: BBa_K222000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aequorin
creator: Cristina Vilanova
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _327_
m user id: 0
owner id: 4909
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The aequorin-coelenterazine complex needs calcium to produce light. In the process this calcium enters through a special type of calcium channels present in the cell???s plasma membrane which open or close in response to a change in the transmembrane potential, that is why they are called voltage-dependent calcium channels (VDCC). The equation which describes calcium current through these channels is the following: Where g is the conductance associated with the channel, V is the transmembrane potential and ECa is the Nernst potential, related to the different ionic concentration inside and outside the cell. Considering that these channels are only permeable to calcium and have two states -open or closed-, the total conductance associated with the population of VDCCs can be expressed as the maximal conductance ( ) times the fraction of all channels that are open. This fraction is determined by hypothetical activation and inactivation variables m and h, which depend on voltage and time: VDCCs are well characterized in excitable cells such as neurons, where the value of is known: Budding yeast, Saccharomyces cerevisiae has homologous voltage-dependent channels in its plasma membrane. However, there is a lack of study of the properties of these channels. That is why we have determined their conductance according to our experimental results: Having characterized calcium entry to the cell, we studied the aequorin production of light through a calcium-mediated response after two types of stimulation: ??? Chemical stimulation Alkali shock (performed by the addition of KOH) induced a calcium entry to cytoplasm (where aequorin is located) through voltage-dependent calcium channels. These calcium ions bind the aequorin-coelenterazine complex and photons ???light- are emitted during a short period of time. This graph shows yeasts??? response to the addition of 30 ??L of KOH: Furthermore, we made experiments with increasing KOH volumes (in microliters) and realized that light em…
mutable notes: -
mutable provenance: It comes from the genomic sequence of the luminiscent jellyfish Aequorea victoria.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K222000_sequence/1

BBa_K531006
type: ComponentDefinition
persistent identity: BBa K531006
display id: BBa_K531006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Optimized esp gene for C. crescentus
creator: Qimeng Gao
created: 2011-07-25T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
group u list: _697_
m user id: 0
owner id: 8860
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The wild Type esp is secreted by S. epidermidis, a bacteria that has low G/C content in its genome. It is then optimized to give a high G/C content without changing any amino acids sequence.
mutable notes: DNA codons are optimized for C. crescentus expression.
mutable provenance: Wild type esp sequence from Zhang,Y.Q., Ren,S.X., Li,H.L., Wang,Y.X., Fu,G., Yang,J., Qin,Z.Q., Miao,Y.G., Wang,W.Y., Chen,R.S., Shen,Y., Chen,Z., Yuan,Z.H., Zhao,G.P., Qu,D., Danchin,A. and Wen,Y.M. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49 (6), 1577-1593 (2003). Optimized sequence is based on the wild type sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K531006_sequence/1

BBa_P10304
type: ComponentDefinition
persistent identity: BBa P10304
display id: BBa_P10304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tRFP
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:59:01Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP80004
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10304_sequence/1

BBa_K331030
type: ComponentDefinition
persistent identity: BBa K331030
display id: BBa_K331030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Repressible N-terminal Arg Tagged EYFP (no terminator)
creator: Adam Smith
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is BBa_K331022 with the tetracycline repressible promoter BBa_R0040 added in front. This will be a component of a proof-of-concept part That will show localization of proteins into the interior of a microcompartment formed by the assembly of lumazine synthase proteins.
mutable notes: To come...
mutable provenance: To come...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K249008
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K249008
sequence: https://synbiohub.org/public/igem/BBa_K331030_sequence/1

BBa_K249008
type: Component
persistent identity: component2083731
display id: component2083731
version: 1
top level: BBa_K331030
access: public
definition: BBa_K249008

BBa_B0034
type: Component
persistent identity: component2083730
display id: component2083730
version: 1
top level: BBa_K331030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2083724
display id: component2083724
version: 1
top level: BBa_K331030
access: public
definition: p(tetR)

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2083724
display id: annotation2083724
version: 1
top level: BBa_K331030
location: https://synbiohub.org/public/igem/BBa_K331030/annotation2083724/range2083724/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K331030/annotation2083724/range2083724/1
type: Range
persistent identity: range2083724
display id: range2083724
version: 1
direction: direction/forward
top level: BBa_K331030
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2083730
display id: annotation2083730
version: 1
top level: BBa_K331030
location: https://synbiohub.org/public/igem/BBa_K331030/annotation2083730/range2083730/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331030/annotation2083730/range2083730/1
type: Range
persistent identity: range2083730
display id: range2083730
version: 1
direction: direction/reverse
top level: BBa_K331030
start: 63
end: 74
orientation: inline

BBa_K249008
type: SequenceAnnotation
persistent identity: annotation2083731
display id: annotation2083731
version: 1
top level: BBa_K331030
location: https://synbiohub.org/public/igem/BBa_K331030/annotation2083731/range2083731/1
component: BBa_K249008

https://synbiohub.org/public/igem/BBa_K331030/annotation2083731/range2083731/1
type: Range
persistent identity: range2083731
display id: range2083731
version: 1
direction: direction/reverse
top level: BBa_K331030
start: 81
end: 842
orientation: inline

BBa_P10300
type: ComponentDefinition
persistent identity: BBa P10300
display id: BBa_P10300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XVE
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:54:46Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP80003
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10300_sequence/1

BBa_K133071
type: ComponentDefinition
persistent identity: BBa K133071
display id: BBa_K133071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UreB
creator: Katja Kolar
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cloned into pSB1AK3 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133071_sequence/1

BBa_J36850
type: ComponentDefinition
persistent identity: BBa J36850
display id: BBa_J36850
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin wild-type + His6 tag
creator: Perry Tsai
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1229
owning group id: 65
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for the core part of wild-type streptavidin protein, without the start codon. Streptavidin protein binds strongly to the biotin molecule, and can be used to bind biotinylated nucleic acids or peptides. There is a histidine tag added at the end of the coding sequence right before the stop codons. You can probe for this protein with an anti-his(C-term) antibody. It was used as part of a fusion protein with lipoprotein signal peptide and outer membrane protein A transmembrane domains, to express streptavidin on the outer surface of E. coli.
mutable notes: There was originally a XbaI site TCTAGA at nucleotides 118-123 of the part, which was mutated to AGCCGC. It is missing its start codon at the beginning, as the intention was to fuse this with surface display domains upstream. There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.
mutable provenance: PCR off a plasmid obtained from Alice Ting's lab at MIT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_J36837
component: BBa_J36841
component: BBa_J36835
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_J36835
sequence annotation: BBa_B0034
sequence annotation: BBa_J36841
sequence annotation: BBa_J36837
sequence: https://synbiohub.org/public/igem/BBa_J36850_sequence/1

BBa_R0010
type: Component
persistent identity: component1909403
display id: component1909403
version: 1
top level: BBa_J36850
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_J36837
type: Component
persistent identity: component1909413
display id: component1909413
version: 1
top level: BBa_J36850
access: public
definition: https://synbiohub.org/public/igem/BBa_J36837/1

BBa_J36841
type: Component
persistent identity: component1909414
display id: component1909414
version: 1
top level: BBa_J36850
access: public
definition: https://synbiohub.org/public/igem/BBa_J36841/1

BBa_J36835
type: Component
persistent identity: component1909412
display id: component1909412
version: 1
top level: BBa_J36850
access: public
definition: https://synbiohub.org/public/igem/BBa_J36835/1

BBa_B0034
type: Component
persistent identity: component1909411
display id: component1909411
version: 1
top level: BBa_J36850
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1909403
display id: annotation1909403
version: 1
top level: BBa_J36850
location: https://synbiohub.org/public/igem/BBa_J36850/annotation1909403/range1909403/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J36850/annotation1909403/range1909403/1
type: Range
persistent identity: range1909403
display id: range1909403
version: 1
direction: direction/forward
top level: BBa_J36850
start: 1
end: 200
orientation: inline

BBa_J36835
type: SequenceAnnotation
persistent identity: annotation1909412
display id: annotation1909412
version: 1
top level: BBa_J36850
location: https://synbiohub.org/public/igem/BBa_J36850/annotation1909412/range1909412/1
component: BBa_J36835

https://synbiohub.org/public/igem/BBa_J36850/annotation1909412/range1909412/1
type: Range
persistent identity: range1909412
display id: range1909412
version: 1
direction: direction/reverse
top level: BBa_J36850
start: 227
end: 313
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1909411
display id: annotation1909411
version: 1
top level: BBa_J36850
location: https://synbiohub.org/public/igem/BBa_J36850/annotation1909411/range1909411/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J36850/annotation1909411/range1909411/1
type: Range
persistent identity: range1909411
display id: range1909411
version: 1
direction: direction/reverse
top level: BBa_J36850
start: 209
end: 220
orientation: inline

BBa_J36841
type: SequenceAnnotation
persistent identity: annotation1909414
display id: annotation1909414
version: 1
top level: BBa_J36850
location: https://synbiohub.org/public/igem/BBa_J36850/annotation1909414/range1909414/1
component: BBa_J36841

https://synbiohub.org/public/igem/BBa_J36850/annotation1909414/range1909414/1
type: Range
persistent identity: range1909414
display id: range1909414
version: 1
direction: direction/reverse
top level: BBa_J36850
start: 672
end: 1076
orientation: inline

BBa_J36837
type: SequenceAnnotation
persistent identity: annotation1909413
display id: annotation1909413
version: 1
top level: BBa_J36850
location: https://synbiohub.org/public/igem/BBa_J36850/annotation1909413/range1909413/1
component: BBa_J36837

https://synbiohub.org/public/igem/BBa_J36850/annotation1909413/range1909413/1
type: Range
persistent identity: range1909413
display id: range1909413
version: 1
direction: direction/reverse
top level: BBa_J36850
start: 322
end: 663
orientation: inline

CFP
type: ComponentDefinition
persistent identity: BBa K157043
display id: BBa_K157043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP
creator: iGEM Team Freiburg 2008
created: 2009-12-08T12:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CFP under the control of a CMV promoter.
mutable notes: This part was split from its plasmid backbone BBa_K157040.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: CFP
sequence: https://synbiohub.org/public/igem/BBa_K157043_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2064647
display id: annotation2064647
version: 1
top level: CFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K157043/annotation2064647/range2064647/1

https://synbiohub.org/public/igem/BBa_K157043/annotation2064647/range2064647/1
type: Range
persistent identity: range2064647
display id: range2064647
version: 1
direction: direction/forward
top level: CFP
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2064648
display id: annotation2064648
version: 1
top level: CFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K157043/annotation2064648/range2064648/1

https://synbiohub.org/public/igem/BBa_K157043/annotation2064648/range2064648/1
type: Range
persistent identity: range2064648
display id: range2064648
version: 1
direction: direction/forward
top level: CFP
start: 730
end: 732
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2064646
display id: annotation2064646
version: 1
top level: CFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157043/annotation2064646/range2064646/1

https://synbiohub.org/public/igem/BBa_K157043/annotation2064646/range2064646/1
type: Range
persistent identity: range2064646
display id: range2064646
version: 1
direction: direction/forward
top level: CFP
start: 1
end: 732
orientation: inline

BBa_K404314
type: ComponentDefinition
persistent identity: BBa K404314
display id: BBa_K404314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DARPin-E01
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: DARPin-E01
sequence: https://synbiohub.org/public/igem/BBa_K404314_sequence/1

DARPin-E01
type: SequenceAnnotation
persistent identity: annotation2094760
display id: annotation2094760
version: 1
top level: BBa_K404314
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404314/annotation2094760/range2094760/1

https://synbiohub.org/public/igem/BBa_K404314/annotation2094760/range2094760/1
type: Range
persistent identity: range2094760
display id: range2094760
version: 1
direction: direction/forward
top level: BBa_K404314
start: 1
end: 459
orientation: inline

BBa_K331033
type: ComponentDefinition
persistent identity: BBa K331033
display id: BBa_K331033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Repressible C-terminal Arg Tagged ECFP (no terminator)
creator: Adam Smith
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is BBa_K331025 with the tetracycline repressible promoter BBa_R0040 added in front. This will be a component of a proof-of-concept part that will show localization of proteins into the interior of a microcompartment formed by the assembly of lumazine synthase proteins.
mutable notes: To come..
mutable provenance: To come....
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K331002
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_K331002
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K331033_sequence/1

BBa_K331002
type: Component
persistent identity: component2083761
display id: component2083761
version: 1
top level: BBa_K331033
access: public
definition: https://synbiohub.org/public/igem/BBa_K331002/1

BBa_B0034
type: Component
persistent identity: component2083757
display id: component2083757
version: 1
top level: BBa_K331033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2083751
display id: component2083751
version: 1
top level: BBa_K331033
access: public
definition: p(tetR)

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2083751
display id: annotation2083751
version: 1
top level: BBa_K331033
location: https://synbiohub.org/public/igem/BBa_K331033/annotation2083751/range2083751/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K331033/annotation2083751/range2083751/1
type: Range
persistent identity: range2083751
display id: range2083751
version: 1
direction: direction/forward
top level: BBa_K331033
start: 1
end: 54
orientation: inline

BBa_K331002
type: SequenceAnnotation
persistent identity: annotation2083761
display id: annotation2083761
version: 1
top level: BBa_K331033
location: https://synbiohub.org/public/igem/BBa_K331033/annotation2083761/range2083761/1
component: BBa_K331002

https://synbiohub.org/public/igem/BBa_K331033/annotation2083761/range2083761/1
type: Range
persistent identity: range2083761
display id: range2083761
version: 1
direction: direction/reverse
top level: BBa_K331033
start: 81
end: 836
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2083757
display id: annotation2083757
version: 1
top level: BBa_K331033
location: https://synbiohub.org/public/igem/BBa_K331033/annotation2083757/range2083757/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331033/annotation2083757/range2083757/1
type: Range
persistent identity: range2083757
display id: range2083757
version: 1
direction: direction/reverse
top level: BBa_K331033
start: 63
end: 74
orientation: inline

ARL2A0402
type: ComponentDefinition
persistent identity: BBa I732113
display id: BBa_I732113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0402
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732113_sequence/1

BBa_K575004
type: ComponentDefinition
persistent identity: BBa K575004
display id: BBa_K575004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Continuous expression of LasR receptor protein (with RBS, B0030)
creator: Nirmit Desai
created: 2011-08-10T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _746_
m user id: 0
owner id: 8746
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: With RBS B0030.
mutable notes: Optimal strength of constitutive promoter.
mutable provenance: Composite part consisting of individual parts in the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_J23119
component: BBa_C0179
sequence annotation: BBa_B0030
sequence annotation: BBa_C0179
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_K575004_sequence/1

BBa_B0030
type: Component
persistent identity: component2124800
display id: component2124800
version: 1
top level: BBa_K575004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23119
type: Component
persistent identity: component2124798
display id: component2124798
version: 1
top level: BBa_K575004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_C0179
type: Component
persistent identity: component2124802
display id: component2124802
version: 1
top level: BBa_K575004
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2124800
display id: annotation2124800
version: 1
top level: BBa_K575004
location: https://synbiohub.org/public/igem/BBa_K575004/annotation2124800/range2124800/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K575004/annotation2124800/range2124800/1
type: Range
persistent identity: range2124800
display id: range2124800
version: 1
direction: direction/forward
top level: BBa_K575004
start: 44
end: 58
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2124802
display id: annotation2124802
version: 1
top level: BBa_K575004
location: https://synbiohub.org/public/igem/BBa_K575004/annotation2124802/range2124802/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K575004/annotation2124802/range2124802/1
type: Range
persistent identity: range2124802
display id: range2124802
version: 1
direction: direction/reverse
top level: BBa_K575004
start: 65
end: 787
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2124798
display id: annotation2124798
version: 1
top level: BBa_K575004
location: https://synbiohub.org/public/igem/BBa_K575004/annotation2124798/range2124798/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K575004/annotation2124798/range2124798/1
type: Range
persistent identity: range2124798
display id: range2124798
version: 1
direction: direction/reverse
top level: BBa_K575004
start: 1
end: 35
orientation: inline

BBa_K929202
type: ComponentDefinition
persistent identity: BBa K929202
display id: BBa_K929202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EGF-Receptor_3rd domain with PelB leadersequence, His-tag and C-terminal Sortase motif
creator: Potsdam Bioware 2012 iGEM
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14461
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PelB EGF-Receptor His-Tag
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AgeI
sequence annotation: EGFR 3rd domain
sequence annotation: PelB leadersequence
sequence annotation: C-terminal sortase motif
sequence annotation: CMV+RBS
sequence annotation: His-tag
sequence annotation: NgoMIV
sequence annotation: ApaI
sequence: https://synbiohub.org/public/igem/BBa_K929202_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation2197685
display id: annotation2197685
version: 1
top level: BBa_K929202
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K929202/annotation2197685/range2197685/1

https://synbiohub.org/public/igem/BBa_K929202/annotation2197685/range2197685/1
type: Range
persistent identity: range2197685
display id: range2197685
version: 1
direction: direction/forward
top level: BBa_K929202
start: 1342
end: 1347
orientation: inline

EGFR 3rd domain
type: SequenceAnnotation
persistent identity: annotation2197656
display id: annotation2197656
version: 1
top level: BBa_K929202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K929202/annotation2197656/range2197656/1

https://synbiohub.org/public/igem/BBa_K929202/annotation2197656/range2197656/1
type: Range
persistent identity: range2197656
display id: range2197656
version: 1
direction: direction/forward
top level: BBa_K929202
start: 733
end: 1308
orientation: inline

PelB leadersequence
type: SequenceAnnotation
persistent identity: annotation2197629
display id: annotation2197629
version: 1
top level: BBa_K929202
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K929202/annotation2197629/range2197629/1

https://synbiohub.org/public/igem/BBa_K929202/annotation2197629/range2197629/1
type: Range
persistent identity: range2197629
display id: range2197629
version: 1
direction: direction/forward
top level: BBa_K929202
start: 670
end: 732
orientation: inline

C-terminal sortase motif
type: SequenceAnnotation
persistent identity: annotation2197677
display id: annotation2197677
version: 1
top level: BBa_K929202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K929202/annotation2197677/range2197677/1

https://synbiohub.org/public/igem/BBa_K929202/annotation2197677/range2197677/1
type: Range
persistent identity: range2197677
display id: range2197677
version: 1
direction: direction/forward
top level: BBa_K929202
start: 1327
end: 1341
orientation: inline

CMV+RBS
type: SequenceAnnotation
persistent identity: annotation2197622
display id: annotation2197622
version: 1
top level: BBa_K929202
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K929202/annotation2197622/range2197622/1

https://synbiohub.org/public/igem/BBa_K929202/annotation2197622/range2197622/1
type: Range
persistent identity: range2197622
display id: range2197622
version: 1
direction: direction/forward
top level: BBa_K929202
start: 1
end: 654
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2197657
display id: annotation2197657
version: 1
top level: BBa_K929202
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K929202/annotation2197657/range2197657/1

https://synbiohub.org/public/igem/BBa_K929202/annotation2197657/range2197657/1
type: Range
persistent identity: range2197657
display id: range2197657
version: 1
direction: direction/forward
top level: BBa_K929202
start: 1309
end: 1326
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2197625
display id: annotation2197625
version: 1
top level: BBa_K929202
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K929202/annotation2197625/range2197625/1

https://synbiohub.org/public/igem/BBa_K929202/annotation2197625/range2197625/1
type: Range
persistent identity: range2197625
display id: range2197625
version: 1
direction: direction/forward
top level: BBa_K929202
start: 664
end: 669
orientation: inline

ApaI
type: SequenceAnnotation
persistent identity: annotation2197689
display id: annotation2197689
version: 1
top level: BBa_K929202
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K929202/annotation2197689/range2197689/1

https://synbiohub.org/public/igem/BBa_K929202/annotation2197689/range2197689/1
type: Range
persistent identity: range2197689
display id: range2197689
version: 1
direction: direction/forward
top level: BBa_K929202
start: 1281
end: 1286
orientation: inline

BBa_J04671
type: ComponentDefinition
persistent identity: BBa J04671
display id: BBa_J04671
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BBa_E0032 EYFP (RBS-, LVA+) and B0015 (Intermidiate Building Sequence)
creator: Oscar Hernandez and Matt Gemberling
created: 2005-11-02T12:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: false
group u list: _16_
m user id: 0
owner id: 435
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an EYFP coding sequence plus a LVA tag and the Terminator Sequence B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0032
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J04671_sequence/1

BBa_E0032
type: Component
persistent identity: component1734637
display id: component1734637
version: 1
top level: BBa_J04671
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0010
type: Component
persistent identity: component1734645
display id: component1734645
version: 1
top level: BBa_J04671
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1734655
display id: component1734655
version: 1
top level: BBa_J04671
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1734655
display id: annotation1734655
version: 1
top level: BBa_J04671
location: https://synbiohub.org/public/igem/BBa_J04671/annotation1734655/range1734655/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04671/annotation1734655/range1734655/1
type: Range
persistent identity: range1734655
display id: range1734655
version: 1
direction: direction/forward
top level: BBa_J04671
start: 859
end: 899
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1734637
display id: annotation1734637
version: 1
top level: BBa_J04671
location: https://synbiohub.org/public/igem/BBa_J04671/annotation1734637/range1734637/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J04671/annotation1734637/range1734637/1
type: Range
persistent identity: range1734637
display id: range1734637
version: 1
direction: direction/forward
top level: BBa_J04671
start: 1
end: 762
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1734645
display id: annotation1734645
version: 1
top level: BBa_J04671
location: https://synbiohub.org/public/igem/BBa_J04671/annotation1734645/range1734645/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04671/annotation1734645/range1734645/1
type: Range
persistent identity: range1734645
display id: range1734645
version: 1
direction: direction/forward
top level: BBa_J04671
start: 771
end: 850
orientation: inline

BBa_K364309
type: ComponentDefinition
persistent identity: BBa K364309
display id: BBa_K364309
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human ER LBD
creator: Lior Malka
created: 2010-09-28T11:00:00Z
modified: 2015-05-08T01:12:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _486_
m user id: 0
owner id: 6778
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Estrogen receptor refers to a group of receptors that are activated by the hormone 17β-estradiol(estrogen). member of the nuclear hormone family of intracellular receptors.
mutable notes: The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25
mutable provenance: Human ER (of the nuclear hormone receptor superfamily)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K364309_sequence/1

BBa_P10202
type: ComponentDefinition
persistent identity: BBa P10202
display id: BBa_P10202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: omega TMV + RAmy3A
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:53:40Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks parts from plasmid pUAP78141
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10202_sequence/1

BBa_K395400
type: ComponentDefinition
persistent identity: BBa K395400
display id: BBa_K395400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-lacIM1-ter
creator: Mitsuhiko Odera
created: 2010-10-04T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _505_
m user id: 0
owner id: 6396
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: resister this part since the sequence of BBa_K082026 is wrong.lacIM1 is a codon-modified lacI designed to reduce the likelihood of recombination with the wild-type lacI(Subhayu Basu,et al.,2005).
mutable notes: test
mutable provenance: AAAGAGGAGAAATACTAGATGAAGCCGGTGACCCTGTATGACGTGGCGGAATACGCGGGCGTGAGCTACCAAACGGTGAGCCGTGTTGTTAATCAAGCGTCTCATGTGAGCGCCAAGACCCGCGAGAAGGTTGAGGCCGCCATGGCCGAATTAAACTATATCCCGAATCGTGTTGCGCAGCAGTTAGCCGGTAAGCAAAGCCTGTTAATCGGTGTGGCGACGAGCAGCTTAGCGTCACATGCCCCAAGCCAGATCGTGGCCGCCATCAAGAGCCGTGCGGACCAGTTAGGCGCGTCTGTTGTTGTTAGAATGGTGGAGCGCTCTGGTGTGGAGGCGTGCAAGGCCGCCGTTCATAACCTGCTGGCCCAGCGTGTGAGCGGCTTAATTATCAATTACCCATTAGACGATCAAGACGCGATCGCGGTTGAGGCGGCGTGTACCAACGTGCCAGCCCTGTTCCTGGACGTGAGCGATCAAACCCCGATTAATAGCATCATCTTTAGCCACGAGAATGGCACCCGCTTAGGTGTTGAACACTTAGTGGCCCTGGGCCATCAACAGATTGCCCTACTGGCCGGTCCGCTGAGCAGCGTGAGCGCCCGCTTACGCTTAGCGGGTTGGCACAAGTACCTGACCCGTAACCAGATCCAACCAATTGCCGAGCGCGAGGGTGATTGGAGCGCGATGAGCGGCTTCCAGCAGACGATGCAGATGTTAAACGAAGGTATTGTGCCGACCGCCATGTTAGTGGCGAATGACCAAATGGCCTTAGGTGCGATGCGTGCGATCACGGAAAGCGGCTTACGTGTGGGCGCCGACATTAGCGTGGTTGGCTATGATGACACGGAGGATTCTAGCTGCTACATTCCACCAAGCACGACGATTAAGCAAGACTTCCGTTTATTAGGCCAGACGTCTGTTGATCGTCTGTTACAGCTGAGCCAAGGTCAAGCCGTTAAAGGTAACCAATTACTGCCGGTGAGCTTAGTTAAGCGCAAGACGACGTTAGCCCCGAACACCCAGACGGCGAGCCCGCGTGCCCTGGCGGACAGCCTGATGCAATTAGCGCGCCAAGTGAGCCGCTTAGAGTCTGGCCAATGATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_K082005
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K082005
sequence: https://synbiohub.org/public/igem/BBa_K395400_sequence/1

BBa_B0010
type: Component
persistent identity: component2082237
display id: component2082237
version: 1
top level: BBa_K395400
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2082239
display id: component2082239
version: 1
top level: BBa_K395400
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2082235
display id: component2082235
version: 1
top level: BBa_K395400
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K082005
type: Component
persistent identity: component2082236
display id: component2082236
version: 1
top level: BBa_K395400
access: public
definition: https://synbiohub.org/public/igem/BBa_K082005/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2082237
display id: annotation2082237
version: 1
top level: BBa_K395400
location: https://synbiohub.org/public/igem/BBa_K395400/annotation2082237/range2082237/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K395400/annotation2082237/range2082237/1
type: Range
persistent identity: range2082237
display id: range2082237
version: 1
direction: direction/forward
top level: BBa_K395400
start: 1110
end: 1189
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2082235
display id: annotation2082235
version: 1
top level: BBa_K395400
location: https://synbiohub.org/public/igem/BBa_K395400/annotation2082235/range2082235/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K395400/annotation2082235/range2082235/1
type: Range
persistent identity: range2082235
display id: range2082235
version: 1
direction: direction/reverse
top level: BBa_K395400
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2082239
display id: annotation2082239
version: 1
top level: BBa_K395400
location: https://synbiohub.org/public/igem/BBa_K395400/annotation2082239/range2082239/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K395400/annotation2082239/range2082239/1
type: Range
persistent identity: range2082239
display id: range2082239
version: 1
direction: direction/forward
top level: BBa_K395400
start: 1198
end: 1238
orientation: inline

BBa_K082005
type: SequenceAnnotation
persistent identity: annotation2082236
display id: annotation2082236
version: 1
top level: BBa_K395400
location: https://synbiohub.org/public/igem/BBa_K395400/annotation2082236/range2082236/1
component: BBa_K082005

https://synbiohub.org/public/igem/BBa_K395400/annotation2082236/range2082236/1
type: Range
persistent identity: range2082236
display id: range2082236
version: 1
direction: direction/reverse
top level: BBa_K395400
start: 19
end: 1101
orientation: inline

BBa_K1189009
type: ComponentDefinition
persistent identity: BBa K1189009
display id: BBa_K1189009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-lactamase with His Tag
creator: Taylor Remondini
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1502_
m user id: 0
owner id: 13453
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdasdas
mutable notes: dasdasdas
mutable provenance: adasdasd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: BBa_J18909
sequence annotation: PolyHis
sequence annotation: BsaI cut site (not removed)
sequence annotation: BBa_K1189008
sequence: https://synbiohub.org/public/igem/BBa_K1189009_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2365964
display id: annotation2365964
version: 1
top level: BBa_K1189009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1189009/annotation2365964/range2365964/1

https://synbiohub.org/public/igem/BBa_K1189009/annotation2365964/range2365964/1
type: Range
persistent identity: range2365964
display id: range2365964
version: 1
direction: direction/forward
top level: BBa_K1189009
start: 1
end: 3
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2365967
display id: annotation2365967
version: 1
top level: BBa_K1189009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1189009/annotation2365967/range2365967/1

https://synbiohub.org/public/igem/BBa_K1189009/annotation2365967/range2365967/1
type: Range
persistent identity: range2365967
display id: range2365967
version: 1
direction: direction/reverse
top level: BBa_K1189009
start: 867
end: 884
orientation: inline

PolyHis
type: SequenceAnnotation
persistent identity: annotation2365966
display id: annotation2365966
version: 1
top level: BBa_K1189009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1189009/annotation2365966/range2365966/1

https://synbiohub.org/public/igem/BBa_K1189009/annotation2365966/range2365966/1
type: Range
persistent identity: range2365966
display id: range2365966
version: 1
direction: direction/forward
top level: BBa_K1189009
start: 867
end: 884
orientation: inline

BsaI cut site (not removed)
type: SequenceAnnotation
persistent identity: annotation2366242
display id: annotation2366242
version: 1
top level: BBa_K1189009
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1189009/annotation2366242/range2366242/1

https://synbiohub.org/public/igem/BBa_K1189009/annotation2366242/range2366242/1
type: Range
persistent identity: range2366242
display id: range2366242
version: 1
direction: direction/forward
top level: BBa_K1189009
start: 712
end: 717
orientation: inline

BBa_K1189008
type: SequenceAnnotation
persistent identity: annotation2365965
display id: annotation2365965
version: 1
top level: BBa_K1189009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1189009/annotation2365965/range2365965/1

https://synbiohub.org/public/igem/BBa_K1189009/annotation2365965/range2365965/1
type: Range
persistent identity: range2365965
display id: range2365965
version: 1
direction: direction/reverse
top level: BBa_K1189009
start: 1
end: 858
orientation: inline

mCitrine
type: ComponentDefinition
persistent identity: BBa J18931
display id: BBa_J18931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCitrine
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Yellow fluorescent protein mCitrine. ===See also=== * BBa_J18930 ===References=== * Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY. (2001) Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. PMID: 11387331
mutable notes: * starts with consensus VSKGEEL * ends with consensus DELYK
mutable provenance: gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18931_sequence/1

BBa_K1051500
type: ComponentDefinition
persistent identity: BBa K1051500
display id: BBa_K1051500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sic1 mutation
creator: Lin Qiongfen
created: 2013-07-01T11:00:00Z
modified: 2015-05-08T01:08:55Z
discontinued: false
dominant: false
group u list: _1358_
m user id: 0
owner id: 15918
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mutate seven phosphorylation site of Sic1, stopping the cell cycle in the G1 phase.
mutable notes: Mutate seven phosphorylation site of Sic1 and one site of Spe1's restriction site.
mutable provenance: Come from saccharomyces cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1051500_sequence/1

BBa_K500002
type: ComponentDefinition
persistent identity: BBa K500002
display id: BBa_K500002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lignin degradation 3
creator: Bin Jia
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _596_
m user id: 0
owner id: 4808
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: substrate
mutable notes: no
mutable provenance: phanerochaete chrysosporium
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PRO
sequence annotation: Lac
sequence: https://synbiohub.org/public/igem/BBa_K500002_sequence/1

PRO
type: SequenceAnnotation
persistent identity: annotation2104212
display id: annotation2104212
version: 1
top level: BBa_K500002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K500002/annotation2104212/range2104212/1

https://synbiohub.org/public/igem/BBa_K500002/annotation2104212/range2104212/1
type: Range
persistent identity: range2104212
display id: range2104212
version: 1
direction: direction/forward
top level: BBa_K500002
start: 1
end: 3
orientation: inline

Lac
type: SequenceAnnotation
persistent identity: annotation2109107
display id: annotation2109107
version: 1
top level: BBa_K500002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K500002/annotation2109107/range2109107/1

https://synbiohub.org/public/igem/BBa_K500002/annotation2109107/range2109107/1
type: Range
persistent identity: range2109107
display id: range2109107
version: 1
direction: direction/forward
top level: BBa_K500002
start: 4
end: 1557
orientation: inline

BBa_K216003
type: ComponentDefinition
persistent identity: BBa K216003
display id: BBa_K216003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TNT receptor TNT-R3
creator: Edinburgh iGEM 2009
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: TNT-R3: a computationally designed receptor binding TNT, made by mutation of the Escherichia coli periplasmic ribose-binding protein: see Looger, L., Dwyer, M.A., Smit, J.J., and Wellinga, H.W. 2003. Computational design of receptor proteins with novel functions. Nature 423, 185-190. This receptor interacts with a hybrid transmembrane protein Trz, created by combining the normal partner of ribose-binding-protein, Trg, with the intracellular part of 2-component sensor protein EnvZ, which normally phosphorylates response regulator protein OmpR, allowing it to activate promoters such as PompC (see Baumgartner, J.W., Kim, C., Brisette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg thate recognizes sugar-binding proteins to the kinase/phosphatase domainof Osmosensor EnvZ. J. Bacteriol. 176, 1157-1163). To use this receptor, include it in a system with Trz and a reporter gene controlled by PompC.To avoid cross-talk, it may be necessary to use a chassis lacking EnvZ and ribose-binding protein.
mutable notes: No special considerations.
mutable provenance: Derived by mutagenesis of the Escherichia coli periplasmic ribose-binding protein: see Looger, L., Dwyer, M.A., Smit, J.J., and Wellinga, H.W. 2003. Computational design of receptor proteins with novel functions. Nature 423, 185-190.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: START
sequence annotation: RBS
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K216003_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2027065
display id: annotation2027065
version: 1
top level: BBa_K216003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K216003/annotation2027065/range2027065/1

https://synbiohub.org/public/igem/BBa_K216003/annotation2027065/range2027065/1
type: Range
persistent identity: range2027065
display id: range2027065
version: 1
direction: direction/forward
top level: BBa_K216003
start: 12
end: 14
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2027064
display id: annotation2027064
version: 1
top level: BBa_K216003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K216003/annotation2027064/range2027064/1

https://synbiohub.org/public/igem/BBa_K216003/annotation2027064/range2027064/1
type: Range
persistent identity: range2027064
display id: range2027064
version: 1
direction: direction/forward
top level: BBa_K216003
start: 1
end: 7
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2027066
display id: annotation2027066
version: 1
top level: BBa_K216003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K216003/annotation2027066/range2027066/1

https://synbiohub.org/public/igem/BBa_K216003/annotation2027066/range2027066/1
type: Range
persistent identity: range2027066
display id: range2027066
version: 1
direction: direction/forward
top level: BBa_K216003
start: 846
end: 851
orientation: inline

BBa_K292006
type: ComponentDefinition
persistent identity: BBa K292006
display id: BBa_K292006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS + LacI repressor + double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: true
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. The LacI repressor represses pLac promoter from the E.coli lactose operon. So LacI repressor and this bio-brick are really reliable to use when we want to control a gene expression by using pLac promoter. In fact when LacI is not expressed, pLac is activated, and when there is an expression of LacI, pLac is inhibited. We can easily design a system to induce the transcription of LacI by using another operon as the tetracycline operon and creates a failed feedback mechanism for pLac inhibition and so, for gene expression inhibition. The LacI (BBa_K292006) already contains a strong RBS and a double terminator, so this brick is ready to use! History: In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
mutable notes: N/A
mutable provenance: Part:BBa_B0030, Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab Part:BBa_C0012, Designed by Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton Group: Registry Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_B0014
component: BBa_C0012
sequence annotation: BBa_B0014
sequence annotation: BBa_C0012
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K292006_sequence/1

BBa_B0030
type: Component
persistent identity: component2249929
display id: component2249929
version: 1
top level: BBa_K292006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0014
type: Component
persistent identity: component2249941
display id: component2249941
version: 1
top level: BBa_K292006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_C0012
type: Component
persistent identity: component2249932
display id: component2249932
version: 1
top level: BBa_K292006
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2249941
display id: annotation2249941
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249941/range2249941/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K292006/annotation2249941/range2249941/1
type: Range
persistent identity: range2249941
display id: range2249941
version: 1
direction: direction/reverse
top level: BBa_K292006
start: 1183
end: 1277
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2249932
display id: annotation2249932
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249932/range2249932/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K292006/annotation2249932/range2249932/1
type: Range
persistent identity: range2249932
display id: range2249932
version: 1
direction: direction/forward
top level: BBa_K292006
start: 22
end: 1149
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249929
display id: annotation2249929
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249929/range2249929/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292006/annotation2249929/range2249929/1
type: Range
persistent identity: range2249929
display id: range2249929
version: 1
direction: direction/forward
top level: BBa_K292006
start: 1
end: 15
orientation: inline

BBa_P10001
type: ComponentDefinition
persistent identity: BBa P10001
display id: BBa_P10001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pro+5U_At6GAL4UAS
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T05:42:40Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP12013
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10001_sequence/1

BBa_K542000
type: ComponentDefinition
persistent identity: BBa K542000
display id: BBa_K542000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lumazine Synthase with Transciptional Terminator (No Promoter)
creator: Anthony Vuong
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Composite part: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002] + [http://partsregistry.org/wiki/index.php/Part:BBa_B0010 BBa_B0010] + [http://partsregistry.org/wiki/index.php/Part:BBa_B0010 BBa_B0010] + [http://partsregistry.org/wiki/index.php/Part:BBa_B0012 BBa_B0012] Made by assembling [http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002] with [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Since this part is lacking the promoter, Lumazine Synthase production may be regulated by the addition different promoters upstream. Regulation of Lumazine Synthase will be dependent on the promoter being utilized.
mutable notes: Intermediate part to assemble the "Co-localization Construct".
mutable provenance: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002] and [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] were obtained from iGEM distribution plates.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_K249002
sequence annotation: BBa_B0015
sequence annotation: BBa_K249002
sequence: https://synbiohub.org/public/igem/BBa_K542000_sequence/1

BBa_B0015
type: Component
persistent identity: component2137880
display id: component2137880
version: 1
top level: BBa_K542000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K249002
type: Component
persistent identity: component2137873
display id: component2137873
version: 1
top level: BBa_K542000
access: public
definition: BBa_K249002

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2137880
display id: annotation2137880
version: 1
top level: BBa_K542000
location: https://synbiohub.org/public/igem/BBa_K542000/annotation2137880/range2137880/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K542000/annotation2137880/range2137880/1
type: Range
persistent identity: range2137880
display id: range2137880
version: 1
direction: direction/reverse
top level: BBa_K542000
start: 474
end: 602
orientation: inline

BBa_K249002
type: SequenceAnnotation
persistent identity: annotation2137873
display id: annotation2137873
version: 1
top level: BBa_K542000
location: https://synbiohub.org/public/igem/BBa_K542000/annotation2137873/range2137873/1
component: BBa_K249002

https://synbiohub.org/public/igem/BBa_K542000/annotation2137873/range2137873/1
type: Range
persistent identity: range2137873
display id: range2137873
version: 1
direction: direction/reverse
top level: BBa_K542000
start: 1
end: 465
orientation: inline

BBa_I757011
type: ComponentDefinition
persistent identity: BBa I757011
display id: BBa_I757011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bla_frag1(aa 26-199) Fusion Part
creator: Freiburg iGEM 2007 team
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _125_
m user id: 0
owner id: 2006
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: * first fragment of the split enzyme beta-lactamase * NgoMIV / AgeI protein fusion part * iGEM Team Freiburg 2007
mutable notes: between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions
mutable provenance: synthetic DNA by GeneArt
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: bla_frag1
sequence: https://synbiohub.org/public/igem/BBa_I757011_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation1954442
display id: annotation1954442
version: 1
top level: BBa_I757011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I757011/annotation1954442/range1954442/1

https://synbiohub.org/public/igem/BBa_I757011/annotation1954442/range1954442/1
type: Range
persistent identity: range1954442
display id: range1954442
version: 1
direction: direction/forward
top level: BBa_I757011
start: 535
end: 540
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation1954441
display id: annotation1954441
version: 1
top level: BBa_I757011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I757011/annotation1954441/range1954441/1

https://synbiohub.org/public/igem/BBa_I757011/annotation1954441/range1954441/1
type: Range
persistent identity: range1954441
display id: range1954441
version: 1
direction: direction/forward
top level: BBa_I757011
start: 4
end: 9
orientation: inline

bla_frag1
type: SequenceAnnotation
persistent identity: annotation1954942
display id: annotation1954942
version: 1
top level: BBa_I757011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I757011/annotation1954942/range1954942/1

https://synbiohub.org/public/igem/BBa_I757011/annotation1954942/range1954942/1
type: Range
persistent identity: range1954942
display id: range1954942
version: 1
direction: direction/forward
top level: BBa_I757011
start: 10
end: 534
orientation: inline

BBa_K258004
type: ComponentDefinition
persistent identity: BBa K258004
display id: BBa_K258004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Keratinocyte growth factor (KGF)
creator: Ozkan IS
created: 2009-10-03T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Keratinocyte growth factor (KGF) is a locally acting epithelial mitogen that is produced by cells of mesenchymal origin and has an important role in protecting and repairing epithelial tissues.Use of recombinant human KGF (palifermin) in patients with hematologic malignancies reduces the incidence and duration of severe oral mucositis experienced after intensive chemoradiotherapy. These results suggest that KGF may be useful in the treatment of patients with other kinds of tumors, including those of epithelial origin.[1]The potential effect of KGF on wound healing was assessed in vitro by measuring randomized migration and plasminogen activator (PA) activity of keratinocytes in response to the growth factor. Incubation of normal human keratinocytes with KGF in modified MCDB 153 medium significantly stimulated cell migration[2] The Keratinocyte Growth Factor (KGF), also known as FGF7, is a growth factor present in the epithelialization-phase of wound healing. In this phase, keratinocytes are covering the wound, forming the epithelium.[3] . KGF is weakly expressed in human skin, but is strongly upregulated in dermal fibroblasts after skin injury. Its binding to a transmembrane receptor on keratinocytes induces proliferation and migration of these cells. Furthermore, KGF has been shown to protect epithelial cells from the toxic effects of reactive oxygen species. We have identified a series of KGF-regulated genes that are likely to play a role in these processes. In addition to KGF, activin seems to be a novel player in wound healing.[4]
mutable notes: The gene is in pMA vector having biobrick restriction sites(Ecor1, Xba1 and Spe1,Pst1,respectively)
mutable provenance: Gene was synthesized at GENEART.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K258004_sequence/1

BBa_P10305
type: ComponentDefinition
persistent identity: BBa P10305
display id: BBa_P10305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T06:00:10Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PhytoBricks part from plasmid pUAP50008
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10305_sequence/1

IFP
type: ComponentDefinition
persistent identity: BBa K256008
display id: BBa_K256008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Infrared Protein (IFP 1.4)
creator: iGEM09_NTU-Singapore
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Sequence from Tsien's lab; Code for the expression of Infrared protein; must co-express with Heme Oxygenase-1 (HO1) to produce infrared signal
mutable notes: --
mutable provenance: Tsien's lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K256008_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2056817
display id: annotation2056817
version: 1
top level: IFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K256008/annotation2056817/range2056817/1

https://synbiohub.org/public/igem/BBa_K256008/annotation2056817/range2056817/1
type: Range
persistent identity: range2056817
display id: range2056817
version: 1
direction: direction/forward
top level: IFP
start: 1
end: 969
orientation: inline

LRLc
type: ComponentDefinition
persistent identity: BBa I732103
display id: BBa_I732103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLc
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732103_sequence/1

BBa_K407008
type: ComponentDefinition
persistent identity: BBa K407008
display id: BBa_K407008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter + RBS + ecfp
creator: Syed Habib Tahir Bukhari, Ashwini Rahul Akkineni, Adithya Ananth, Victor Gordeev, Svea Grieb, Sarah Mansour, Mareike Roth, Charanya Sampathkumar, Lucas Schirmer, Jonathan Tam
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _521_
m user id: 0
owner id: 7360
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a Tetracycline repressive promoter, RBS and the reporter ecfp (cyan fluorescent protein).
mutable notes: Will express ecfp constitutively without repression of pTet
mutable provenance: genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0034
component: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K407008_sequence/1

BBa_R0040
type: Component
persistent identity: component2096066
display id: component2096066
version: 1
top level: BBa_K407008
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component2096072
display id: component2096072
version: 1
top level: BBa_K407008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component2096073
display id: component2096073
version: 1
top level: BBa_K407008
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2096072
display id: annotation2096072
version: 1
top level: BBa_K407008
location: https://synbiohub.org/public/igem/BBa_K407008/annotation2096072/range2096072/1
component: BBa_B0034

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type: Range
persistent identity: range2096072
display id: range2096072
version: 1
direction: direction/reverse
top level: BBa_K407008
start: 63
end: 74
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2096073
display id: annotation2096073
version: 1
top level: BBa_K407008
location: https://synbiohub.org/public/igem/BBa_K407008/annotation2096073/range2096073/1
component: BBa_E0020

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type: Range
persistent identity: range2096073
display id: range2096073
version: 1
direction: direction/reverse
top level: BBa_K407008
start: 81
end: 803
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2096066
display id: annotation2096066
version: 1
top level: BBa_K407008
location: https://synbiohub.org/public/igem/BBa_K407008/annotation2096066/range2096066/1
component: BBa_R0040

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type: Range
persistent identity: range2096066
display id: range2096066
version: 1
direction: direction/forward
top level: BBa_K407008
start: 1
end: 54
orientation: inline

BBa_I732092
type: ComponentDefinition
persistent identity: BBa I732092
display id: BBa_I732092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Repoters (LacZ-alpha and RFP)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The activity of upstream promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I732018
component: BBa_B0015
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_I732018
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I732092_sequence/1

BBa_I732018
type: Component
persistent identity: component2261109
display id: component2261109
version: 1
top level: BBa_I732092
access: public
definition: https://synbiohub.org/public/igem/BBa_I732018/1

BBa_B0015
type: Component
persistent identity: component2261121
display id: component2261121
version: 1
top level: BBa_I732092
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2261111
display id: component2261111
version: 1
top level: BBa_I732092
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2261114
display id: component2261114
version: 1
top level: BBa_I732092
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2261111
display id: annotation2261111
version: 1
top level: BBa_I732092
location: https://synbiohub.org/public/igem/BBa_I732092/annotation2261111/range2261111/1
component: BBa_B0034

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type: Range
persistent identity: range2261111
display id: range2261111
version: 1
direction: direction/reverse
top level: BBa_I732092
start: 261
end: 272
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2261114
display id: annotation2261114
version: 1
top level: BBa_I732092
location: https://synbiohub.org/public/igem/BBa_I732092/annotation2261114/range2261114/1
component: BBa_E1010

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type: Range
persistent identity: range2261114
display id: range2261114
version: 1
direction: direction/reverse
top level: BBa_I732092
start: 279
end: 984
orientation: inline

BBa_I732018
type: SequenceAnnotation
persistent identity: annotation2261109
display id: annotation2261109
version: 1
top level: BBa_I732092
location: https://synbiohub.org/public/igem/BBa_I732092/annotation2261109/range2261109/1
component: BBa_I732018

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type: Range
persistent identity: range2261109
display id: range2261109
version: 1
direction: direction/reverse
top level: BBa_I732092
start: 1
end: 252
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2261121
display id: annotation2261121
version: 1
top level: BBa_I732092
location: https://synbiohub.org/public/igem/BBa_I732092/annotation2261121/range2261121/1
component: BBa_B0015

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type: Range
persistent identity: range2261121
display id: range2261121
version: 1
direction: direction/reverse
top level: BBa_I732092
start: 993
end: 1121
orientation: inline

BBa_K133139
type: ComponentDefinition
persistent identity: BBa K133139
display id: BBa_K133139
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: multiHP
creator: Katja Kolar
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133139_sequence/1

BBa_K274374
type: ComponentDefinition
persistent identity: BBa K274374
display id: BBa_K274374
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> P2 ogr - Psid promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746350
component: BBa_I746364
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_I746364
sequence annotation: BBa_B0012
sequence annotation: BBa_I746350
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K274374_sequence/1

BBa_I746350
type: Component
persistent identity: component2055306
display id: component2055306
version: 1
top level: BBa_K274374
access: public
definition: https://synbiohub.org/public/igem/BBa_I746350/1

BBa_I746364
type: Component
persistent identity: component2055314
display id: component2055314
version: 1
top level: BBa_K274374
access: public
definition: https://synbiohub.org/public/igem/BBa_I746364/1

BBa_B0010
type: Component
persistent identity: component2055307
display id: component2055307
version: 1
top level: BBa_K274374
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2055309
display id: component2055309
version: 1
top level: BBa_K274374
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746364
type: SequenceAnnotation
persistent identity: annotation2055314
display id: annotation2055314
version: 1
top level: BBa_K274374
location: https://synbiohub.org/public/igem/BBa_K274374/annotation2055314/range2055314/1
component: BBa_I746364

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type: Range
persistent identity: range2055314
display id: range2055314
version: 1
direction: direction/reverse
top level: BBa_K274374
start: 383
end: 475
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055309
display id: annotation2055309
version: 1
top level: BBa_K274374
location: https://synbiohub.org/public/igem/BBa_K274374/annotation2055309/range2055309/1
component: BBa_B0012

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type: Range
persistent identity: range2055309
display id: range2055309
version: 1
direction: direction/forward
top level: BBa_K274374
start: 334
end: 374
orientation: inline

BBa_I746350
type: SequenceAnnotation
persistent identity: annotation2055306
display id: annotation2055306
version: 1
top level: BBa_K274374
location: https://synbiohub.org/public/igem/BBa_K274374/annotation2055306/range2055306/1
component: BBa_I746350

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type: Range
persistent identity: range2055306
display id: range2055306
version: 1
direction: direction/reverse
top level: BBa_K274374
start: 1
end: 237
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055307
display id: annotation2055307
version: 1
top level: BBa_K274374
location: https://synbiohub.org/public/igem/BBa_K274374/annotation2055307/range2055307/1
component: BBa_B0010

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type: Range
persistent identity: range2055307
display id: range2055307
version: 1
direction: direction/forward
top level: BBa_K274374
start: 246
end: 325
orientation: inline

BBa_K091171
type: ComponentDefinition
persistent identity: BBa K091171
display id: BBa_K091171
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+RBS+cI+TT
creator: Xiao Zhu
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: for lambda cI.
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_P0455
component: BBa_I13453
sequence annotation: BBa_P0455
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K091171_sequence/1

BBa_P0455
type: Component
persistent identity: component2254241
display id: component2254241
version: 1
top level: BBa_K091171
access: public
definition: BBa_P0455

BBa_I13453
type: Component
persistent identity: component2254228
display id: component2254228
version: 1
top level: BBa_K091171
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_P0455
type: SequenceAnnotation
persistent identity: annotation2254241
display id: annotation2254241
version: 1
top level: BBa_K091171
location: https://synbiohub.org/public/igem/BBa_K091171/annotation2254241/range2254241/1
component: BBa_P0455

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type: Range
persistent identity: range2254241
display id: range2254241
version: 1
direction: direction/reverse
top level: BBa_K091171
start: 139
end: 1034
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2254228
display id: annotation2254228
version: 1
top level: BBa_K091171
location: https://synbiohub.org/public/igem/BBa_K091171/annotation2254228/range2254228/1
component: BBa_I13453

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type: Range
persistent identity: range2254228
display id: range2254228
version: 1
direction: direction/reverse
top level: BBa_K091171
start: 1
end: 130
orientation: inline

BBa_J70312
type: ComponentDefinition
persistent identity: BBa J70312
display id: BBa_J70312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E0031 (RBS+EYFP), B0014 (terminator)
creator: Julie Norville
created: 2009-06-16T11:00:00Z
modified: 2015-05-08T01:08:21Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: analogue of BBa_I13507 (rbs+FPa, terminator) using a new fluorescent protein and terminator
mutable notes: none
mutable provenance: E0031 and B0014
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0012
component: BBa_B0011
component: BBa_E0031
sequence annotation: BBa_E0031
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_J70312_sequence/1

BBa_B0012
type: Component
persistent identity: component2005579
display id: component2005579
version: 1
top level: BBa_J70312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2005583
display id: component2005583
version: 1
top level: BBa_J70312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_E0031
type: Component
persistent identity: component2005578
display id: component2005578
version: 1
top level: BBa_J70312
access: public
definition: https://synbiohub.org/public/igem/BBa_E0031/1

BBa_E0031
type: SequenceAnnotation
persistent identity: annotation2005578
display id: annotation2005578
version: 1
top level: BBa_J70312
location: https://synbiohub.org/public/igem/BBa_J70312/annotation2005578/range2005578/1
component: BBa_E0031

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type: Range
persistent identity: range2005578
display id: range2005578
version: 1
direction: direction/reverse
top level: BBa_J70312
start: 1
end: 740
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2005579
display id: annotation2005579
version: 1
top level: BBa_J70312
location: https://synbiohub.org/public/igem/BBa_J70312/annotation2005579/range2005579/1
component: BBa_B0012

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type: Range
persistent identity: range2005579
display id: range2005579
version: 1
direction: direction/forward
top level: BBa_J70312
start: 749
end: 789
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2005583
display id: annotation2005583
version: 1
top level: BBa_J70312
location: https://synbiohub.org/public/igem/BBa_J70312/annotation2005583/range2005583/1
component: BBa_B0011

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type: Range
persistent identity: range2005583
display id: range2005583
version: 1
direction: direction/forward
top level: BBa_J70312
start: 798
end: 843
orientation: inline

BBa_K352001
type: ComponentDefinition
persistent identity: BBa K352001
display id: BBa_K352001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CooA from Rhodospirillum rubrum
creator: Cihan Tastan
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _474_606_
m user id: 0
owner id: 5815
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. CooA is a member of a family of transcriptional regulators similar to the cAMP receptor protein and fumavate nitrate reduction from Escherichia coli. The protein is active in sequence-specific DNA binding in the presence of CO, but not in the absence of CO. The protein to be a dimer in the absence of CO. The product, CooA, is 28% identical (51% similar) to CRP(cAMP receptor protein) and 18% identical (45% similar) to FNR(fumavate nitrate reduction) from Escherichia coli. Inactive Fe(II) CooA structure adapted from that of the strain with PDB identification no. 1FT9. The protein consists of two monomers, shaded differently, which dimerize along the central C-helices of adjacent effector-binding domains. The solved structure is asymmetric, in which one monomer contains fused C- and D-helices. Nonetheless, both F-helices that interact with DNA in a sequence-specific manner are buried from the surface in the structure. The 4/5 loop is noted and so are the Pro2 and His77 heme Fe(II) ligands.
mutable notes: The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.
mutable provenance: Hwan Youn, Robert L. Kerby, Mary Conrad, et al. 2004. Functionally Critical Elements of CooA-Related CO Sensors. J. Bacteriol. 186(5):1320-1329. doi:10.1128/JB.186.5.1320-1329.2004.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K352001_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2081251
display id: annotation2081251
version: 1
top level: BBa_K352001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K352001/annotation2081251/range2081251/1

https://synbiohub.org/public/igem/BBa_K352001/annotation2081251/range2081251/1
type: Range
persistent identity: range2081251
display id: range2081251
version: 1
direction: direction/forward
top level: BBa_K352001
start: 1
end: 3
orientation: inline

BBa_K1031441
type: ComponentDefinition
persistent identity: BBa K1031441
display id: BBa_K1031441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23106-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 106-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: HcaR
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K1031441_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357454
display id: annotation2357454
version: 1
top level: BBa_K1031441
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357454/range2357454/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357454/range2357454/1
type: Range
persistent identity: range2357454
display id: range2357454
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 44
end: 55
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357455
display id: annotation2357455
version: 1
top level: BBa_K1031441
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357455/range2357455/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357455/range2357455/1
type: Range
persistent identity: range2357455
display id: range2357455
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 62
end: 952
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2357453
display id: annotation2357453
version: 1
top level: BBa_K1031441
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357453/range2357453/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357453/range2357453/1
type: Range
persistent identity: range2357453
display id: range2357453
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 1
end: 35
orientation: inline

BBa_K352011
type: ComponentDefinition
persistent identity: BBa K352011
display id: BBa_K352011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCooM Promoter coupled with RBS+RFP+Double terminator
creator: Ş.Serap Memik
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _474_
m user id: 0
owner id: 6078
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pCooM Promoter coupled with RBS, RFP,double terminator
mutable notes: Device created from a composite of parts.
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K352003
sequence annotation: BBa_K352003
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K352011_sequence/1

BBa_I13507
type: Component
persistent identity: component2258097
display id: component2258097
version: 1
top level: BBa_K352011
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K352003
type: Component
persistent identity: component2258085
display id: component2258085
version: 1
top level: BBa_K352011
access: public
definition: https://synbiohub.org/public/igem/BBa_K352003/1

BBa_K352003
type: SequenceAnnotation
persistent identity: annotation2258085
display id: annotation2258085
version: 1
top level: BBa_K352011
location: https://synbiohub.org/public/igem/BBa_K352011/annotation2258085/range2258085/1
component: BBa_K352003

https://synbiohub.org/public/igem/BBa_K352011/annotation2258085/range2258085/1
type: Range
persistent identity: range2258085
display id: range2258085
version: 1
direction: direction/reverse
top level: BBa_K352011
start: 1
end: 66
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2258097
display id: annotation2258097
version: 1
top level: BBa_K352011
location: https://synbiohub.org/public/igem/BBa_K352011/annotation2258097/range2258097/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K352011/annotation2258097/range2258097/1
type: Range
persistent identity: range2258097
display id: range2258097
version: 1
direction: direction/reverse
top level: BBa_K352011
start: 75
end: 935
orientation: inline

BBa_K777001
type: ComponentDefinition
persistent identity: BBa K777001
display id: BBa_K777001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23100
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K777000
sequence annotation: BBa_K777000
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K777001_sequence/1

BBa_J23100
type: Component
persistent identity: component2189122
display id: component2189122
version: 1
top level: BBa_K777001
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K777000
type: Component
persistent identity: component2189126
display id: component2189126
version: 1
top level: BBa_K777001
access: public
definition: BBa_K777000

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189126
display id: annotation2189126
version: 1
top level: BBa_K777001
location: https://synbiohub.org/public/igem/BBa_K777001/annotation2189126/range2189126/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777001/annotation2189126/range2189126/1
type: Range
persistent identity: range2189126
display id: range2189126
version: 1
direction: direction/reverse
top level: BBa_K777001
start: 42
end: 1703
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189122
display id: annotation2189122
version: 1
top level: BBa_K777001
location: https://synbiohub.org/public/igem/BBa_K777001/annotation2189122/range2189122/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777001/annotation2189122/range2189122/1
type: Range
persistent identity: range2189122
display id: range2189122
version: 1
direction: direction/reverse
top level: BBa_K777001
start: 1
end: 35
orientation: inline

BBa_K1033932
type: ComponentDefinition
persistent identity: BBa K1033932
display id: BBa_K1033932
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, pink chromoprotein
creator: Erik Lundin
created: 2014-04-20T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This chromoprotein from the coral Stylophora pistillata, spisPink (also known as spisCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 560 nm giving it a pink color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein spisPink has significant sequence homologies with proteins in the GFP family.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Stylophora pistillata. The protein was first extracted and characterized by Alieva et. al. under the name spisCP (GenBank: ABB17971.1). This version is codon optimized for E coli by Genscript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spisPink
sequence: https://synbiohub.org/public/igem/BBa_K1033932_sequence/1

spisPink
type: SequenceAnnotation
persistent identity: annotation2372666
display id: annotation2372666
version: 1
top level: BBa_K1033932
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033932/annotation2372666/range2372666/1

https://synbiohub.org/public/igem/BBa_K1033932/annotation2372666/range2372666/1
type: Range
persistent identity: range2372666
display id: range2372666
version: 1
direction: direction/forward
top level: BBa_K1033932
start: 1
end: 678
orientation: inline

cI
type: ComponentDefinition
persistent identity: BBa I9200
display id: BBa_I9200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI lambda
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: cI sequence slightly different from part C0051. NCBI locus: NC_001416
mutable provenance: lambda bacteriophage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I9200_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation302655
display id: annotation302655
version: 1
top level: cI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I9200/annotation302655/range302655/1

https://synbiohub.org/public/igem/BBa_I9200/annotation302655/range302655/1
type: Range
persistent identity: range302655
display id: range302655
version: 1
direction: direction/forward
top level: cI
start: 1
end: 717
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214008
display id: annotation2214008
version: 1
top level: cI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I9200/annotation2214008/range2214008/1

https://synbiohub.org/public/igem/BBa_I9200/annotation2214008/range2214008/1
type: Range
persistent identity: range2214008
display id: range2214008
version: 1
direction: direction/forward
top level: cI
start: 718
end: 742
orientation: inline

BBa_K112624
type: ComponentDefinition
persistent identity: BBa K112624
display id: BBa_K112624
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {Pbad}.{rbs_pelB>}.{<phoA>} in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is assembled with pBad promoter, rbs.pelB> (pelB prepro with native rbs and start codon, but no stop), and a <phoA> region (no start or stop codon). ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: This part was made using AKL41, which consisted of {Pbad}.{rbs_pelB>}.{<phoA!}.{<b1006>}. The stop codon of the phoA region and the <b1006> terminator were removed. PCR with ca998 Forward oligo, and mea37 reverse oligo for {<phoA>}.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112624_sequence/1

BBa_J33207
type: ComponentDefinition
persistent identity: BBa J33207
display id: BBa_J33207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lac promoter and lacZ
creator: Chris French
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part (submitted in pSB1A2) consists of the lac promoter and lacZ' gene, encoding the N-terminal 76 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation for blue-white selection on Xgal plates. A SacI site has been introduced at the 3' end, overlapping the XbaI site of the Biobrick prefix. This is designed to be used as a cloning vector for making new biobricks. PCR primers can be designed with a SacI site in one primer and an SpeI site in the other. This removes the necessity for an excessively long non-complementary tail on one primer bearing either the full biobrick prefix or suffix. The PCR product can then be digested with SacI and SpeI for insertion into this plasmid, replacing the Plac-lacZ' cassette. Recombinant plasmids will then be white on IPTG/Xgal plates, whereas any that still contain the original insert will be blue. We have used this strategy to prepare several biobricks, including BBa_J33204, which contains the xylE gene encoding catechol-2,3-dioxygenase. (For making biobricks that contain lacZ', BBa_J33204 can be used in the same way; in this case, clones with plasmids that still contain xylE will turn yellow on addition of a drop of 10 mM catechol.)
mutable notes: Note that the SacI site overlaps the SpeI site. The Biobrick prefix ends ...TCTAGAG. When this is added to the CTC at the start of the sequence shown here, the SacI site, GAGCTC, is generated.
mutable provenance: The DNA was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). The annotation shown here is based on that associated with this Genbank entry. The sequence shown here is derived by sequencing the construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lacZ'
sequence annotation: -10
sequence annotation: rbs
sequence annotation: LacI binding site
sequence annotation: SacI
sequence annotation: -35
sequence annotation: CAP binding site
sequence: https://synbiohub.org/public/igem/BBa_J33207_sequence/1

lacZ'
type: SequenceAnnotation
persistent identity: annotation1907858
display id: annotation1907858
version: 1
top level: BBa_J33207
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907858/range1907858/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907858/range1907858/1
type: Range
persistent identity: range1907858
display id: range1907858
version: 1
direction: direction/forward
top level: BBa_J33207
start: 370
end: 600
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1907859
display id: annotation1907859
version: 1
top level: BBa_J33207
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907859/range1907859/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907859/range1907859/1
type: Range
persistent identity: range1907859
display id: range1907859
version: 1
direction: direction/forward
top level: BBa_J33207
start: 320
end: 325
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1907857
display id: annotation1907857
version: 1
top level: BBa_J33207
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907857/range1907857/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907857/range1907857/1
type: Range
persistent identity: range1907857
display id: range1907857
version: 1
direction: direction/forward
top level: BBa_J33207
start: 359
end: 362
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1907856
display id: annotation1907856
version: 1
top level: BBa_J33207
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907856/range1907856/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907856/range1907856/1
type: Range
persistent identity: range1907856
display id: range1907856
version: 1
direction: direction/forward
top level: BBa_J33207
start: 332
end: 352
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1907854
display id: annotation1907854
version: 1
top level: BBa_J33207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907854/range1907854/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907854/range1907854/1
type: Range
persistent identity: range1907854
display id: range1907854
version: 1
direction: direction/forward
top level: BBa_J33207
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1907860
display id: annotation1907860
version: 1
top level: BBa_J33207
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907860/range1907860/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907860/range1907860/1
type: Range
persistent identity: range1907860
display id: range1907860
version: 1
direction: direction/forward
top level: BBa_J33207
start: 297
end: 302
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1907855
display id: annotation1907855
version: 1
top level: BBa_J33207
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907855/range1907855/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907855/range1907855/1
type: Range
persistent identity: range1907855
display id: range1907855
version: 1
direction: direction/forward
top level: BBa_J33207
start: 248
end: 285
orientation: inline

Δ15de
type: ComponentDefinition
persistent identity: BBa K925001
display id: BBa_K925001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Delta 15 desaturase
creator: Veronica Rezelj
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1190_
m user id: 0
owner id: 12788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a delta-15 desaturase derived from Synechocystis sp PCC 6803. The enzyme is able to introduce a double bond at the Δ-15 site in the hydrocarbon chain of linoleic acid (18:2 ; Δ9,12), for its conversion into alpha- linoleic acid (18:3 ; Δ9,12,15), an Omega-3 polyunsaturated fatty acid (PUFA). Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part in addition to delta-12 desaturase (BBa_K925000), alpha-linoleic acid is observed in membrane lipids.
mutable notes: .
mutable provenance: This desaturase comes from Synechocystis sp PCC 6803 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: d15des
sequence: https://synbiohub.org/public/igem/BBa_K925001_sequence/1

d15des
type: SequenceAnnotation
persistent identity: annotation2196209
display id: annotation2196209
version: 1
top level: Δ15de
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K925001/annotation2196209/range2196209/1

https://synbiohub.org/public/igem/BBa_K925001/annotation2196209/range2196209/1
type: Range
persistent identity: range2196209
display id: range2196209
version: 1
direction: direction/forward
top level: Δ15de
start: 1
end: 1080
orientation: inline

RFP-BA-Bbs
type: ComponentDefinition
persistent identity: BBa K349003
display id: BBa_K349003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-BA-BbsI (for construction of BA BioBytes 2.0)
creator: David Lloyd
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _453_
m user id: 0
owner id: 4034
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Test plasmid for the construction of new Bytes-v2. The RFP cassette can be removed using the BbsI enzyme and a new ORF or cassette may be inserted into the plasmid allowing for testing of the part with the BioBytes Assembly System-v2.
mutable notes: None
mutable provenance: pSB1C3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A
sequence annotation: j04450
sequence annotation: BbsI
sequence annotation: B
sequence: https://synbiohub.org/public/igem/BBa_K349003_sequence/1

A
type: SequenceAnnotation
persistent identity: annotation2110098
display id: annotation2110098
version: 1
top level: RFP-BA-Bbs
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K349003/annotation2110098/range2110098/1

https://synbiohub.org/public/igem/BBa_K349003/annotation2110098/range2110098/1
type: Range
persistent identity: range2110098
display id: range2110098
version: 1
direction: direction/reverse
top level: RFP-BA-Bbs
start: 1082
end: 1085
orientation: inline

j04450
type: SequenceAnnotation
persistent identity: annotation2100721
display id: annotation2100721
version: 1
top level: RFP-BA-Bbs
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K349003/annotation2100721/range2100721/1

https://synbiohub.org/public/igem/BBa_K349003/annotation2100721/range2100721/1
type: Range
persistent identity: range2100721
display id: range2100721
version: 1
direction: direction/forward
top level: RFP-BA-Bbs
start: 13
end: 1081
orientation: inline

BbsI
type: SequenceAnnotation
persistent identity: annotation2110097
display id: annotation2110097
version: 1
top level: RFP-BA-Bbs
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K349003/annotation2110097/range2110097/1

https://synbiohub.org/public/igem/BBa_K349003/annotation2110097/range2110097/1
type: Range
persistent identity: range2110097
display id: range2110097
version: 1
direction: direction/reverse
top level: RFP-BA-Bbs
start: 1088
end: 1093
orientation: inline

BbsI
type: SequenceAnnotation
persistent identity: annotation2110095
display id: annotation2110095
version: 1
top level: RFP-BA-Bbs
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K349003/annotation2110095/range2110095/1

https://synbiohub.org/public/igem/BBa_K349003/annotation2110095/range2110095/1
type: Range
persistent identity: range2110095
display id: range2110095
version: 1
direction: direction/forward
top level: RFP-BA-Bbs
start: 1
end: 6
orientation: inline

B
type: SequenceAnnotation
persistent identity: annotation2110096
display id: annotation2110096
version: 1
top level: RFP-BA-Bbs
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K349003/annotation2110096/range2110096/1

https://synbiohub.org/public/igem/BBa_K349003/annotation2110096/range2110096/1
type: Range
persistent identity: range2110096
display id: range2110096
version: 1
direction: direction/forward
top level: RFP-BA-Bbs
start: 9
end: 12
orientation: inline

ClpX
type: ComponentDefinition
persistent identity: BBa K365004
display id: BBa_K365004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ClpX from E.coli (aa 61-425)
creator: RENAULT Renaud
created: 2010-09-13T11:00:00Z
modified: 2015-05-08T01:12:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _477_
m user id: 0
owner id: 6082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: In wild type E. coli, ClpX forms a ring of hexamer and binds to a double heptamer of ClpP. ClpX recognizes a specific C-terminal degradation tag called SsrA and starts to unfold the tagged protein. The denatured polypeptide is translocated into the ClpP subunit, which breaks peptide bonds. The N terminal end of ClpX contains a domain which is responsible for interaction with its natural adaptator protein (SspB) that we would avoid. As this N-terminal domain is also partially responsible for ClpX subunits complexation into an hexamer, fusing three C-terminal end of ClpX together with appropriate linkers increases the stability of the system (T. Baker et al.) in the absence of this N-terminal domain.
mutable notes: We deleted 2 EcoRI sites and 2 Age1 sites by directed mutagenis at positions 522 and 967, and 183 and 921 respectively. This biobrick has standard RFC-25.
mutable provenance: Genomic sequence from E.coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C > T
sequence annotation: A > G
sequence: https://synbiohub.org/public/igem/BBa_K365004_sequence/1

C > T
type: SequenceAnnotation
persistent identity: annotation2079948
display id: annotation2079948
version: 1
top level: ClpX
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K365004/annotation2079948/range2079948/1

https://synbiohub.org/public/igem/BBa_K365004/annotation2079948/range2079948/1
type: Range
persistent identity: range2079948
display id: range2079948
version: 1
direction: direction/forward
top level: ClpX
start: 921
end: 921
orientation: inline

C > T
type: SequenceAnnotation
persistent identity: annotation2079945
display id: annotation2079945
version: 1
top level: ClpX
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K365004/annotation2079945/range2079945/1

https://synbiohub.org/public/igem/BBa_K365004/annotation2079945/range2079945/1
type: Range
persistent identity: range2079945
display id: range2079945
version: 1
direction: direction/forward
top level: ClpX
start: 183
end: 183
orientation: inline

A > G
type: SequenceAnnotation
persistent identity: annotation2079946
display id: annotation2079946
version: 1
top level: ClpX
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K365004/annotation2079946/range2079946/1

https://synbiohub.org/public/igem/BBa_K365004/annotation2079946/range2079946/1
type: Range
persistent identity: range2079946
display id: range2079946
version: 1
direction: direction/forward
top level: ClpX
start: 522
end: 522
orientation: inline

A > G
type: SequenceAnnotation
persistent identity: annotation2079947
display id: annotation2079947
version: 1
top level: ClpX
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K365004/annotation2079947/range2079947/1

https://synbiohub.org/public/igem/BBa_K365004/annotation2079947/range2079947/1
type: Range
persistent identity: range2079947
display id: range2079947
version: 1
direction: direction/forward
top level: ClpX
start: 867
end: 867
orientation: inline

BBa_K360021
type: ComponentDefinition
persistent identity: BBa K360021
display id: BBa_K360021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LovTAP: Blue Light Photosensor and Transcriptional Regulator
creator: Claudia Ivonne Hernandez Armenta
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _485_
m user id: 0
owner id: 6618
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: hola
mutable notes: sip
mutable provenance: wow
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K360021_sequence/1

BBa_K173019
type: ComponentDefinition
persistent identity: BBa K173019
display id: BBa_K173019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: adhB protein generator
creator: Lorenzo Pasotti, Susanna Zucca, Paolo Magni
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _281_
m user id: 0
owner id: 2583
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_K173017
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence annotation: BBa_K173017
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K173019_sequence/1

BBa_B0030
type: Component
persistent identity: component2044277
display id: component2044277
version: 1
top level: BBa_K173019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K173017
type: Component
persistent identity: component2044279
display id: component2044279
version: 1
top level: BBa_K173019
access: public
definition: https://synbiohub.org/public/igem/BBa_K173017/1

BBa_B0010
type: Component
persistent identity: component2044280
display id: component2044280
version: 1
top level: BBa_K173019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2044282
display id: component2044282
version: 1
top level: BBa_K173019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2044277
display id: annotation2044277
version: 1
top level: BBa_K173019
location: https://synbiohub.org/public/igem/BBa_K173019/annotation2044277/range2044277/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K173019/annotation2044277/range2044277/1
type: Range
persistent identity: range2044277
display id: range2044277
version: 1
direction: direction/forward
top level: BBa_K173019
start: 1
end: 15
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2044280
display id: annotation2044280
version: 1
top level: BBa_K173019
location: https://synbiohub.org/public/igem/BBa_K173019/annotation2044280/range2044280/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K173019/annotation2044280/range2044280/1
type: Range
persistent identity: range2044280
display id: range2044280
version: 1
direction: direction/forward
top level: BBa_K173019
start: 1185
end: 1264
orientation: inline

BBa_K173017
type: SequenceAnnotation
persistent identity: annotation2044279
display id: annotation2044279
version: 1
top level: BBa_K173019
location: https://synbiohub.org/public/igem/BBa_K173019/annotation2044279/range2044279/1
component: BBa_K173017

https://synbiohub.org/public/igem/BBa_K173019/annotation2044279/range2044279/1
type: Range
persistent identity: range2044279
display id: range2044279
version: 1
direction: direction/reverse
top level: BBa_K173019
start: 22
end: 1176
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2044282
display id: annotation2044282
version: 1
top level: BBa_K173019
location: https://synbiohub.org/public/igem/BBa_K173019/annotation2044282/range2044282/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K173019/annotation2044282/range2044282/1
type: Range
persistent identity: range2044282
display id: range2044282
version: 1
direction: direction/forward
top level: BBa_K173019
start: 1273
end: 1313
orientation: inline

BBa_I714894
type: ComponentDefinition
persistent identity: BBa I714894
display id: BBa_I714894
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SDA_CI
creator: Chunbo Lou
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SDA+CI coding region
mutable notes: another repressor in our switch,
mutable provenance: lambda genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: lambda CI
sequence annotation: SDA
sequence: https://synbiohub.org/public/igem/BBa_I714894_sequence/1

lambda CI
type: SequenceAnnotation
persistent identity: annotation1951569
display id: annotation1951569
version: 1
top level: BBa_I714894
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714894/annotation1951569/range1951569/1

https://synbiohub.org/public/igem/BBa_I714894/annotation1951569/range1951569/1
type: Range
persistent identity: range1951569
display id: range1951569
version: 1
direction: direction/reverse
top level: BBa_I714894
start: 1
end: 714
orientation: inline

SDA
type: SequenceAnnotation
persistent identity: annotation1951568
display id: annotation1951568
version: 1
top level: BBa_I714894
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I714894/annotation1951568/range1951568/1

https://synbiohub.org/public/igem/BBa_I714894/annotation1951568/range1951568/1
type: Range
persistent identity: range1951568
display id: range1951568
version: 1
direction: direction/reverse
top level: BBa_I714894
start: 715
end: 726
orientation: inline

BBa_K309020
type: ComponentDefinition
persistent identity: BBa K309020
display id: BBa_K309020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP (for use in C. elegans chassis)
creator: Thai Phi
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:11:53Z
discontinued: false
dominant: false
group u list: _428_
m user id: 0
owner id: 6985
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a reporter for use in a C. elegans chassis. Excitation wavelength of 434 nm and emission wavelength of 477 nm.
mutable notes: This part was PCRed out of a plasmid.
mutable provenance: Genetic mutant of GFP (Green Fluorescent Protein) from Aequorea victoria. (pIC517).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Start Codon
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K309020_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2108982
display id: annotation2108982
version: 1
top level: BBa_K309020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K309020/annotation2108982/range2108982/1

https://synbiohub.org/public/igem/BBa_K309020/annotation2108982/range2108982/1
type: Range
persistent identity: range2108982
display id: range2108982
version: 1
direction: direction/forward
top level: BBa_K309020
start: 10
end: 12
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2108971
display id: annotation2108971
version: 1
top level: BBa_K309020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K309020/annotation2108971/range2108971/1

https://synbiohub.org/public/igem/BBa_K309020/annotation2108971/range2108971/1
type: Range
persistent identity: range2108971
display id: range2108971
version: 1
direction: direction/forward
top level: BBa_K309020
start: 1
end: 9
orientation: inline

BBa_K398018
type: ComponentDefinition
persistent identity: BBa K398018
display id: BBa_K398018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH generator
creator: Kira Schipper
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6322
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: ADH, an alcohol dehydrogenase isolated from Bacillus thermoleovorans B23, is capable of converting n-alkanols into the corresponding n-alkanal, the second step in the biodegradation of alkanes.
mutable notes: Nucleotide sequence optimized for expression in E.coli
mutable provenance: Genebank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J23100
component: BBa_K398005
component: BBa_J61101
sequence annotation: BBa_J23100
sequence annotation: BBa_J61101
sequence annotation: BBa_K398005
sequence: https://synbiohub.org/public/igem/BBa_K398018_sequence/1

BBa_J23100
type: Component
persistent identity: component2083527
display id: component2083527
version: 1
top level: BBa_K398018
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K398005
type: Component
persistent identity: component2083532
display id: component2083532
version: 1
top level: BBa_K398018
access: public
definition: https://synbiohub.org/public/igem/BBa_K398005/1

BBa_J61101
type: Component
persistent identity: component2083528
display id: component2083528
version: 1
top level: BBa_K398018
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2083527
display id: annotation2083527
version: 1
top level: BBa_K398018
location: https://synbiohub.org/public/igem/BBa_K398018/annotation2083527/range2083527/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K398018/annotation2083527/range2083527/1
type: Range
persistent identity: range2083527
display id: range2083527
version: 1
direction: direction/reverse
top level: BBa_K398018
start: 1
end: 35
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2083528
display id: annotation2083528
version: 1
top level: BBa_K398018
location: https://synbiohub.org/public/igem/BBa_K398018/annotation2083528/range2083528/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K398018/annotation2083528/range2083528/1
type: Range
persistent identity: range2083528
display id: range2083528
version: 1
direction: direction/reverse
top level: BBa_K398018
start: 44
end: 55
orientation: inline

BBa_K398005
type: SequenceAnnotation
persistent identity: annotation2083532
display id: annotation2083532
version: 1
top level: BBa_K398018
location: https://synbiohub.org/public/igem/BBa_K398018/annotation2083532/range2083532/1
component: BBa_K398005

https://synbiohub.org/public/igem/BBa_K398018/annotation2083532/range2083532/1
type: Range
persistent identity: range2083532
display id: range2083532
version: 1
direction: direction/reverse
top level: BBa_K398018
start: 62
end: 811
orientation: inline

BBa_K249006
type: ComponentDefinition
persistent identity: BBa K249006
display id: BBa_K249006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion EYFP with C-terminal Arginine Tag
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This Yellow Fluorescent protein has been fused with 10 C-terminal Arginines to target it into the Lumazine-based microcompartment.
mutable notes: Standard
mutable provenance: BioBrick Foundation EYFP fused with a synthesized Arginine tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K249006_sequence/1

sacB
type: ComponentDefinition
persistent identity: BBa K322921
display id: BBa_K322921
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.
creator: Chris French and Maria Kowal
created: 2010-09-23T11:00:00Z
modified: 2015-05-08T01:12:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: sacB encodes the Bacillus subtilis levansucrase,which is the enzyme catalyzing hydrolysis of sucrose and synthesis of levans(high-molecular-weight fructose polymers). It is lethal to gram-negative bacteria E-coli. It works with cat as an alternative method for inserting BioBricks into the genome by using homologous recombination instead of restriction digestion, with the added bonus of not leaving a marker behind in the product. Protocol is shown here: http://2010.igem.org/Team:Edinburgh/Project/Protocol
mutable notes: Note: MABEL was used to mutate an internal EcoRI site to GAGTTC (underlined). Features: coding sequence runs from 13 to 1434, RBS 1 to 3, EcoRI site mutated 211 to 216.
mutable provenance: Source: Bacillus subtilis 168 genomic DNA. Sequence (from SacBPlan11June10.docx)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: Coding sequence
sequence annotation: Removed EcoRI site
sequence: https://synbiohub.org/public/igem/BBa_K322921_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2098459
display id: annotation2098459
version: 1
top level: sacB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K322921/annotation2098459/range2098459/1

https://synbiohub.org/public/igem/BBa_K322921/annotation2098459/range2098459/1
type: Range
persistent identity: range2098459
display id: range2098459
version: 1
direction: direction/forward
top level: sacB
start: 1
end: 3
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2098480
display id: annotation2098480
version: 1
top level: sacB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K322921/annotation2098480/range2098480/1

https://synbiohub.org/public/igem/BBa_K322921/annotation2098480/range2098480/1
type: Range
persistent identity: range2098480
display id: range2098480
version: 1
direction: direction/forward
top level: sacB
start: 13
end: 1434
orientation: inline

Removed EcoRI site
type: SequenceAnnotation
persistent identity: annotation2098481
display id: annotation2098481
version: 1
top level: sacB
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K322921/annotation2098481/range2098481/1

https://synbiohub.org/public/igem/BBa_K322921/annotation2098481/range2098481/1
type: Range
persistent identity: range2098481
display id: range2098481
version: 1
direction: direction/forward
top level: sacB
start: 211
end: 216
orientation: inline

BBa_K554008
type: ComponentDefinition
persistent identity: BBa K554008
display id: BBa_K554008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hemolysin D - HlyD
creator: UNICAMP EMSE Brazil team
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _722_
m user id: 0
owner id: 7971
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: HlyD is ...
mutable provenance: Synthesized sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HlyD
sequence annotation: stop
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K554008_sequence/1

HlyD
type: SequenceAnnotation
persistent identity: annotation2136918
display id: annotation2136918
version: 1
top level: BBa_K554008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K554008/annotation2136918/range2136918/1

https://synbiohub.org/public/igem/BBa_K554008/annotation2136918/range2136918/1
type: Range
persistent identity: range2136918
display id: range2136918
version: 1
direction: direction/forward
top level: BBa_K554008
start: 16
end: 1449
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2136919
display id: annotation2136919
version: 1
top level: BBa_K554008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K554008/annotation2136919/range2136919/1

https://synbiohub.org/public/igem/BBa_K554008/annotation2136919/range2136919/1
type: Range
persistent identity: range2136919
display id: range2136919
version: 1
direction: direction/forward
top level: BBa_K554008
start: 1450
end: 1455
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136917
display id: annotation2136917
version: 1
top level: BBa_K554008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K554008/annotation2136917/range2136917/1

https://synbiohub.org/public/igem/BBa_K554008/annotation2136917/range2136917/1
type: Range
persistent identity: range2136917
display id: range2136917
version: 1
direction: direction/forward
top level: BBa_K554008
start: 1
end: 15
orientation: inline

BBa_K274394
type: ComponentDefinition
persistent identity: BBa K274394
display id: BBa_K274394
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> PhiR73 pag - Psid promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_I746352
component: BBa_I746364
component: BBa_B0010
sequence annotation: BBa_I746364
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I746352
sequence: https://synbiohub.org/public/igem/BBa_K274394_sequence/1

BBa_B0012
type: Component
persistent identity: component2055408
display id: component2055408
version: 1
top level: BBa_K274394
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746352
type: Component
persistent identity: component2055405
display id: component2055405
version: 1
top level: BBa_K274394
access: public
definition: https://synbiohub.org/public/igem/BBa_I746352/1

BBa_I746364
type: Component
persistent identity: component2055413
display id: component2055413
version: 1
top level: BBa_K274394
access: public
definition: https://synbiohub.org/public/igem/BBa_I746364/1

BBa_B0010
type: Component
persistent identity: component2055406
display id: component2055406
version: 1
top level: BBa_K274394
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746364
type: SequenceAnnotation
persistent identity: annotation2055413
display id: annotation2055413
version: 1
top level: BBa_K274394
location: https://synbiohub.org/public/igem/BBa_K274394/annotation2055413/range2055413/1
component: BBa_I746364

https://synbiohub.org/public/igem/BBa_K274394/annotation2055413/range2055413/1
type: Range
persistent identity: range2055413
display id: range2055413
version: 1
direction: direction/reverse
top level: BBa_K274394
start: 410
end: 502
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055408
display id: annotation2055408
version: 1
top level: BBa_K274394
location: https://synbiohub.org/public/igem/BBa_K274394/annotation2055408/range2055408/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274394/annotation2055408/range2055408/1
type: Range
persistent identity: range2055408
display id: range2055408
version: 1
direction: direction/forward
top level: BBa_K274394
start: 361
end: 401
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055406
display id: annotation2055406
version: 1
top level: BBa_K274394
location: https://synbiohub.org/public/igem/BBa_K274394/annotation2055406/range2055406/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274394/annotation2055406/range2055406/1
type: Range
persistent identity: range2055406
display id: range2055406
version: 1
direction: direction/forward
top level: BBa_K274394
start: 273
end: 352
orientation: inline

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2055405
display id: annotation2055405
version: 1
top level: BBa_K274394
location: https://synbiohub.org/public/igem/BBa_K274394/annotation2055405/range2055405/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K274394/annotation2055405/range2055405/1
type: Range
persistent identity: range2055405
display id: range2055405
version: 1
direction: direction/reverse
top level: BBa_K274394
start: 1
end: 264
orientation: inline

BBa_K331031
type: ComponentDefinition
persistent identity: BBa K331031
display id: BBa_K331031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Repressible C-terminal Arg Tagged EYFP (no terminator)
creator: Adam Smith
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is BBa_K331023 with the tetracycline repressible promoter BBa_R0040 added in front. This will be a component of a proof-of-concept part that will show localization of proteins into the interior of a microcompartment formed by the assembly of lumazine synthase proteins.
mutable notes: To come...
mutable provenance: To come...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0040
component: BBa_B0034
component: BBa_K249006
sequence annotation: BBa_B0034
sequence annotation: BBa_K249006
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K331031_sequence/1

BBa_R0040
type: Component
persistent identity: component2083732
display id: component2083732
version: 1
top level: BBa_K331031
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component2083738
display id: component2083738
version: 1
top level: BBa_K331031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K249006
type: Component
persistent identity: component2083739
display id: component2083739
version: 1
top level: BBa_K331031
access: public
definition: BBa_K249006

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2083738
display id: annotation2083738
version: 1
top level: BBa_K331031
location: https://synbiohub.org/public/igem/BBa_K331031/annotation2083738/range2083738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331031/annotation2083738/range2083738/1
type: Range
persistent identity: range2083738
display id: range2083738
version: 1
direction: direction/reverse
top level: BBa_K331031
start: 63
end: 74
orientation: inline

BBa_K249006
type: SequenceAnnotation
persistent identity: annotation2083739
display id: annotation2083739
version: 1
top level: BBa_K331031
location: https://synbiohub.org/public/igem/BBa_K331031/annotation2083739/range2083739/1
component: BBa_K249006

https://synbiohub.org/public/igem/BBa_K331031/annotation2083739/range2083739/1
type: Range
persistent identity: range2083739
display id: range2083739
version: 1
direction: direction/reverse
top level: BBa_K331031
start: 81
end: 836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2083732
display id: annotation2083732
version: 1
top level: BBa_K331031
location: https://synbiohub.org/public/igem/BBa_K331031/annotation2083732/range2083732/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K331031/annotation2083732/range2083732/1
type: Range
persistent identity: range2083732
display id: range2083732
version: 1
direction: direction/forward
top level: BBa_K331031
start: 1
end: 54
orientation: inline

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
creator: Sabriya Rosemond, Erin Zwack
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
mutable notes: It was cloned into PsB1A2 plasmids.
mutable provenance: PsB1AT3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rev primer J31006
sequence annotation: tetA(C) backwards
sequence annotation: stop
sequence annotation: Fwd primer J31006
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
direction: direction/forward
top level: tetA(C)b
start: 1
end: 20
orientation: inline

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
direction: direction/reverse
top level: tetA(C)b
start: 1
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
direction: direction/reverse
top level: tetA(C)b
start: 1
end: 3
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
direction: direction/reverse
top level: tetA(C)b
start: 1168
end: 1191
orientation: inline

BBa_K091109
type: ComponentDefinition
persistent identity: BBa K091109
display id: BBa_K091109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxS
creator: Andrew Gordon
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LuxS is a synthase for the AI-2 quorum sensing system. This protein is involved in the production of small molecules that are readily absorbed by multiple species of bacteria. LuxS can be used as part of a positive feedback loop in cell to cell signaling.
mutable notes: Nucleotide position 54 changed from A to T in order to destroy a PstI site; this is a sense mutation Also changed stop codon from TAG to TAA (recommended in Registry Help)
mutable provenance: The LuxS sequence was pulled from E. coli MG1655 by predesigned primers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091109_sequence/1

BBa_K874000
type: ComponentDefinition
persistent identity: BBa K874000
display id: BBa_K874000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M.ScaI Methyltransferase
creator: Ernst Bank
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1135_
m user id: 0
owner id: 12435
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: The M.ScaI protein is a type II methyltransferase (subtype beta) that recognises site on the DNA of the following sequence 5..AGTACT..3. It methylates this site at the 5th (Cytosine) nucleotide leaving ao an N4-methylcytosine (m4). This methylation type (m4) is not found in native E. coli nor is the recognition site methylated by any of E. coli's native methylation systems (Dam, Dcm). This part also includes a flexible linker with incorporated myc-tag at the N terminus of the protein. This will allow for easier creation of fusion proteins and expression verification using westerblot (using the myc antibodies). It can be assumed that M.ScaI (with linker) is expressed and folds properly in E. coli because its function has been verified by the iGEM Amsterdam 2012 team.
mutable notes: Contains no forbidden sites (according to the RFC-10 protocol). Contains no sites vulnerable to E. coli's native restriction systems.
mutable provenance: Natively found in Streptomyces caespitosus. Sequence obtained from REBASE. Synthesized by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M.ScaI
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K874000_sequence/1

M.ScaI
type: SequenceAnnotation
persistent identity: annotation2187004
display id: annotation2187004
version: 1
top level: BBa_K874000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K874000/annotation2187004/range2187004/1

https://synbiohub.org/public/igem/BBa_K874000/annotation2187004/range2187004/1
type: Range
persistent identity: range2187004
display id: range2187004
version: 1
direction: direction/forward
top level: BBa_K874000
start: 1
end: 912
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2187005
display id: annotation2187005
version: 1
top level: BBa_K874000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K874000/annotation2187005/range2187005/1

https://synbiohub.org/public/igem/BBa_K874000/annotation2187005/range2187005/1
type: Range
persistent identity: range2187005
display id: range2187005
version: 1
direction: direction/forward
top level: BBa_K874000
start: 913
end: 915
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2187003
display id: annotation2187003
version: 1
top level: BBa_K874000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K874000/annotation2187003/range2187003/1

https://synbiohub.org/public/igem/BBa_K874000/annotation2187003/range2187003/1
type: Range
persistent identity: range2187003
display id: range2187003
version: 1
direction: direction/forward
top level: BBa_K874000
start: 1
end: 3
orientation: inline

BBa_K899001
type: ComponentDefinition
persistent identity: BBa K899001
display id: BBa_K899001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fructan fructan fructosyltransferase (FFT)
creator: Andreas Kj??r
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1164_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Elongates a trisaccharide with the fructose unit of a sucrose substrate into an inulin polymer. Works together with part BBa_K899000 (SST).
mutable notes: 7 BP from start codon we embedded an shine dalgarno sequence.
mutable provenance: Extracted from Jesrusalem Artichoke (helianthus tuberosus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K899001_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2193673
display id: annotation2193673
version: 1
top level: BBa_K899001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K899001/annotation2193673/range2193673/1

https://synbiohub.org/public/igem/BBa_K899001/annotation2193673/range2193673/1
type: Range
persistent identity: range2193673
display id: range2193673
version: 1
direction: direction/forward
top level: BBa_K899001
start: 1846
end: 1848
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2193672
display id: annotation2193672
version: 1
top level: BBa_K899001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K899001/annotation2193672/range2193672/1

https://synbiohub.org/public/igem/BBa_K899001/annotation2193672/range2193672/1
type: Range
persistent identity: range2193672
display id: range2193672
version: 1
direction: direction/forward
top level: BBa_K899001
start: 1
end: 3
orientation: inline

SBFP2
type: ComponentDefinition
persistent identity: BBa K156010
display id: BBa_K156010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SBFP2 (strongly enhanced blue fluorescent protein)
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2016-01-26T09:51:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 4206
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part codes for orange fluorescent protein (OFP). Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter.
mutable notes: This part was codon-optimized for expression in ''E. coli''. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: OFP was cloned from the tentacles of ''Cerianthus'' sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156010_sequence/1

BBa_K1124110
type: ComponentDefinition
persistent identity: BBa K1124110
display id: BBa_K1124110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilGFP(+LVA) generator(+RBS, +terminator)
creator: Takahiro Yamada
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coming soon!
mutable notes: Coming soon!
mutable provenance: Coming soon!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1124000
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1124000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1124110_sequence/1

BBa_K1124000
type: Component
persistent identity: component2341936
display id: component2341936
version: 1
top level: BBa_K1124110
access: public
definition: https://synbiohub.org/public/igem/BBa_K1124000/1

BBa_B0015
type: Component
persistent identity: component2341943
display id: component2341943
version: 1
top level: BBa_K1124110
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2341931
display id: component2341931
version: 1
top level: BBa_K1124110
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2341931
display id: annotation2341931
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341931/range2341931/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341931/range2341931/1
type: Range
persistent identity: range2341931
display id: range2341931
version: 1
direction: direction/reverse
top level: BBa_K1124110
start: 1
end: 12
orientation: inline

BBa_K1124000
type: SequenceAnnotation
persistent identity: annotation2341936
display id: annotation2341936
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341936/range2341936/1
component: BBa_K1124000

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341936/range2341936/1
type: Range
persistent identity: range2341936
display id: range2341936
version: 1
direction: direction/reverse
top level: BBa_K1124110
start: 19
end: 756
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2341943
display id: annotation2341943
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341943/range2341943/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341943/range2341943/1
type: Range
persistent identity: range2341943
display id: range2341943
version: 1
direction: direction/reverse
top level: BBa_K1124110
start: 765
end: 893
orientation: inline

BBa_K274384
type: ComponentDefinition
persistent identity: BBa K274384
display id: BBa_K274384
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> PSP3 pag - Psid promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_I746351
component: BBa_I746364
sequence annotation: BBa_I746351
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746364
sequence: https://synbiohub.org/public/igem/BBa_K274384_sequence/1

BBa_B0012
type: Component
persistent identity: component2055369
display id: component2055369
version: 1
top level: BBa_K274384
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2055367
display id: component2055367
version: 1
top level: BBa_K274384
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746351
type: Component
persistent identity: component2055366
display id: component2055366
version: 1
top level: BBa_K274384
access: public
definition: https://synbiohub.org/public/igem/BBa_I746351/1

BBa_I746364
type: Component
persistent identity: component2055374
display id: component2055374
version: 1
top level: BBa_K274384
access: public
definition: https://synbiohub.org/public/igem/BBa_I746364/1

BBa_I746351
type: SequenceAnnotation
persistent identity: annotation2055366
display id: annotation2055366
version: 1
top level: BBa_K274384
location: https://synbiohub.org/public/igem/BBa_K274384/annotation2055366/range2055366/1
component: BBa_I746351

https://synbiohub.org/public/igem/BBa_K274384/annotation2055366/range2055366/1
type: Range
persistent identity: range2055366
display id: range2055366
version: 1
direction: direction/reverse
top level: BBa_K274384
start: 1
end: 237
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055367
display id: annotation2055367
version: 1
top level: BBa_K274384
location: https://synbiohub.org/public/igem/BBa_K274384/annotation2055367/range2055367/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274384/annotation2055367/range2055367/1
type: Range
persistent identity: range2055367
display id: range2055367
version: 1
direction: direction/forward
top level: BBa_K274384
start: 246
end: 325
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055369
display id: annotation2055369
version: 1
top level: BBa_K274384
location: https://synbiohub.org/public/igem/BBa_K274384/annotation2055369/range2055369/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274384/annotation2055369/range2055369/1
type: Range
persistent identity: range2055369
display id: range2055369
version: 1
direction: direction/forward
top level: BBa_K274384
start: 334
end: 374
orientation: inline

BBa_I746364
type: SequenceAnnotation
persistent identity: annotation2055374
display id: annotation2055374
version: 1
top level: BBa_K274384
location: https://synbiohub.org/public/igem/BBa_K274384/annotation2055374/range2055374/1
component: BBa_I746364

https://synbiohub.org/public/igem/BBa_K274384/annotation2055374/range2055374/1
type: Range
persistent identity: range2055374
display id: range2055374
version: 1
direction: direction/reverse
top level: BBa_K274384
start: 383
end: 475
orientation: inline

BBa_K132018
type: ComponentDefinition
persistent identity: BBa K132018
display id: BBa_K132018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC
creator: Du Jin,Hou Nan,Liu Duo,Zhang Weiran,Yuan Fang
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _219_
m user id: 0
owner id: 2835
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: araC+B0010+B0012
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_S03519
sequence annotation: BBa_S03519
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K132018_sequence/1

BBa_B0015
type: Component
persistent identity: component2249368
display id: component2249368
version: 1
top level: BBa_K132018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_S03519
type: Component
persistent identity: component2249361
display id: component2249361
version: 1
top level: BBa_K132018
access: public
definition: https://synbiohub.org/public/igem/BBa_S03519/1

BBa_S03519
type: SequenceAnnotation
persistent identity: annotation2249361
display id: annotation2249361
version: 1
top level: BBa_K132018
location: https://synbiohub.org/public/igem/BBa_K132018/annotation2249361/range2249361/1
component: BBa_S03519

https://synbiohub.org/public/igem/BBa_K132018/annotation2249361/range2249361/1
type: Range
persistent identity: range2249361
display id: range2249361
version: 1
direction: direction/reverse
top level: BBa_K132018
start: 1
end: 958
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249368
display id: annotation2249368
version: 1
top level: BBa_K132018
location: https://synbiohub.org/public/igem/BBa_K132018/annotation2249368/range2249368/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K132018/annotation2249368/range2249368/1
type: Range
persistent identity: range2249368
display id: range2249368
version: 1
direction: direction/reverse
top level: BBa_K132018
start: 967
end: 1095
orientation: inline

BBa_K1129039
type: ComponentDefinition
persistent identity: BBa K1129039
display id: BBa_K1129039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cinnamoyl-CoA reductase from Arabidopsis thaliana under pTET constitutive promoter
creator: UBC iGEM 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1441_
m user id: 0
owner id: 9774
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Arab.+rbs+AtCCR1+term
mutable notes: Arab.+rbs+AtCCR1+term
mutable provenance: genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I13453
component: BBa_K1129005
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_K1129005
sequence annotation: BBa_I13453
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1129039_sequence/1

BBa_I13453
type: Component
persistent identity: component2353719
display id: component2353719
version: 1
top level: BBa_K1129039
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K1129005
type: Component
persistent identity: component2353722
display id: component2353722
version: 1
top level: BBa_K1129039
access: public
definition: https://synbiohub.org/public/igem/BBa_K1129005/1

BBa_B0015
type: Component
persistent identity: component2353729
display id: component2353729
version: 1
top level: BBa_K1129039
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2353721
display id: component2353721
version: 1
top level: BBa_K1129039
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1129005
type: SequenceAnnotation
persistent identity: annotation2353722
display id: annotation2353722
version: 1
top level: BBa_K1129039
location: https://synbiohub.org/public/igem/BBa_K1129039/annotation2353722/range2353722/1
component: BBa_K1129005

https://synbiohub.org/public/igem/BBa_K1129039/annotation2353722/range2353722/1
type: Range
persistent identity: range2353722
display id: range2353722
version: 1
direction: direction/reverse
top level: BBa_K1129039
start: 157
end: 1191
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2353719
display id: annotation2353719
version: 1
top level: BBa_K1129039
location: https://synbiohub.org/public/igem/BBa_K1129039/annotation2353719/range2353719/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1129039/annotation2353719/range2353719/1
type: Range
persistent identity: range2353719
display id: range2353719
version: 1
direction: direction/reverse
top level: BBa_K1129039
start: 1
end: 130
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2353729
display id: annotation2353729
version: 1
top level: BBa_K1129039
location: https://synbiohub.org/public/igem/BBa_K1129039/annotation2353729/range2353729/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1129039/annotation2353729/range2353729/1
type: Range
persistent identity: range2353729
display id: range2353729
version: 1
direction: direction/reverse
top level: BBa_K1129039
start: 1200
end: 1328
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2353721
display id: annotation2353721
version: 1
top level: BBa_K1129039
location: https://synbiohub.org/public/igem/BBa_K1129039/annotation2353721/range2353721/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1129039/annotation2353721/range2353721/1
type: Range
persistent identity: range2353721
display id: range2353721
version: 1
direction: direction/reverse
top level: BBa_K1129039
start: 139
end: 150
orientation: inline

BBa_K390501
type: ComponentDefinition
persistent identity: BBa K390501
display id: BBa_K390501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacPromoter/RBS/PhaP/HlyA/Terminator
creator: Utah State iGEM
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _518_
m user id: 0
owner id: 4773
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Expresses phasin fused to the HlyA signal peptide. Secretion target.
mutable notes: none
mutable provenance: See intermediate parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K208029
component: BBa_K208010
sequence annotation: BBa_K208010
sequence annotation: BBa_K208029
sequence: https://synbiohub.org/public/igem/BBa_K390501_sequence/1

BBa_K208029
type: Component
persistent identity: component2099650
display id: component2099650
version: 1
top level: BBa_K390501
access: public
definition: https://synbiohub.org/public/igem/BBa_K208029/1

BBa_K208010
type: Component
persistent identity: component2099640
display id: component2099640
version: 1
top level: BBa_K390501
access: public
definition: https://synbiohub.org/public/igem/BBa_K208010/1

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2099640
display id: annotation2099640
version: 1
top level: BBa_K390501
location: https://synbiohub.org/public/igem/BBa_K390501/annotation2099640/range2099640/1
component: BBa_K208010

https://synbiohub.org/public/igem/BBa_K390501/annotation2099640/range2099640/1
type: Range
persistent identity: range2099640
display id: range2099640
version: 1
direction: direction/reverse
top level: BBa_K390501
start: 1
end: 220
orientation: inline

BBa_K208029
type: SequenceAnnotation
persistent identity: annotation2099650
display id: annotation2099650
version: 1
top level: BBa_K390501
location: https://synbiohub.org/public/igem/BBa_K390501/annotation2099650/range2099650/1
component: BBa_K208029

https://synbiohub.org/public/igem/BBa_K390501/annotation2099650/range2099650/1
type: Range
persistent identity: range2099650
display id: range2099650
version: 1
direction: direction/reverse
top level: BBa_K390501
start: 227
end: 1136
orientation: inline

BBa_K332033
type: ComponentDefinition
persistent identity: BBa K332033
display id: BBa_K332033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
mutable notes: To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
mutable provenance: the 37℃ induced RBS cames from iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K115002
component: BBa_J61048
component: BBa_C0040
sequence annotation: BBa_K115002
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332033_sequence/1

BBa_K115002
type: Component
persistent identity: component2247611
display id: component2247611
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J61048
type: Component
persistent identity: component2247616
display id: component2247616
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_C0040
type: Component
persistent identity: component2247615
display id: component2247615
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2247611
display id: annotation2247611
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
type: Range
persistent identity: range2247611
display id: range2247611
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 1
end: 52
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2247615
display id: annotation2247615
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
type: Range
persistent identity: range2247615
display id: range2247615
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 59
end: 743
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2247616
display id: annotation2247616
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
type: Range
persistent identity: range2247616
display id: range2247616
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 752
end: 864
orientation: inline

PEST
type: ComponentDefinition
persistent identity: BBa J176018
display id: BBa_J176018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PEST
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: degradation tag
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176018_sequence/1

BBa_P0478
type: ComponentDefinition
persistent identity: BBa P0478
display id: BBa_P0478
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0078.B0015
creator: cconboy
created: 2004-05-24T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_C0078
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0078
sequence: https://synbiohub.org/public/igem/BBa_P0478_sequence/1

BBa_B0012
type: Component
persistent identity: component945180
display id: component945180
version: 1
top level: BBa_P0478
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0078
type: Component
persistent identity: component945164
display id: component945164
version: 1
top level: BBa_P0478
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_B0010
type: Component
persistent identity: component945170
display id: component945170
version: 1
top level: BBa_P0478
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component945154
display id: component945154
version: 1
top level: BBa_P0478
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation945170
display id: annotation945170
version: 1
top level: BBa_P0478
location: https://synbiohub.org/public/igem/BBa_P0478/annotation945170/range945170/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0478/annotation945170/range945170/1
type: Range
persistent identity: range945170
display id: range945170
version: 1
direction: direction/forward
top level: BBa_P0478
start: 694
end: 773
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation945180
display id: annotation945180
version: 1
top level: BBa_P0478
location: https://synbiohub.org/public/igem/BBa_P0478/annotation945180/range945180/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0478/annotation945180/range945180/1
type: Range
persistent identity: range945180
display id: range945180
version: 1
direction: direction/forward
top level: BBa_P0478
start: 782
end: 822
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation945154
display id: annotation945154
version: 1
top level: BBa_P0478
location: https://synbiohub.org/public/igem/BBa_P0478/annotation945154/range945154/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0478/annotation945154/range945154/1
type: Range
persistent identity: range945154
display id: range945154
version: 1
direction: direction/forward
top level: BBa_P0478
start: 1
end: 12
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation945164
display id: annotation945164
version: 1
top level: BBa_P0478
location: https://synbiohub.org/public/igem/BBa_P0478/annotation945164/range945164/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_P0478/annotation945164/range945164/1
type: Range
persistent identity: range945164
display id: range945164
version: 1
direction: direction/forward
top level: BBa_P0478
start: 19
end: 660
orientation: inline

BBa_K676002
type: ComponentDefinition
persistent identity: BBa K676002
display id: BBa_K676002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mNARK with YFP.LVA
creator: Kheng Tee Ng
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:13:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _859_
m user id: 0
owner id: 9831
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A more sensitive oxygen detector in bioreactor. Constructed with mNark promoter and enhanced YFP with LVA tag.
mutable notes: and hence also stronger activity under anaerobic conditions. It is also shorter than the NarK promoter (BBa_K239005)
mutable provenance: mNarK promoter is designed to have better affinity to activated Fnr than the NarK promoter (BBa_K239005) and hence also stronger activity under anaerobic conditions. It is also shorter than the NarK promoter (BBa_K239005).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K239006
component: BBa_E0432
sequence annotation: BBa_E0432
sequence annotation: BBa_K239006
sequence: https://synbiohub.org/public/igem/BBa_K676002_sequence/1

BBa_K239006
type: Component
persistent identity: component2131544
display id: component2131544
version: 1
top level: BBa_K676002
access: public
definition: https://synbiohub.org/public/igem/BBa_K239006/1

BBa_E0432
type: Component
persistent identity: component2131558
display id: component2131558
version: 1
top level: BBa_K676002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0432/1

BBa_E0432
type: SequenceAnnotation
persistent identity: annotation2131558
display id: annotation2131558
version: 1
top level: BBa_K676002
location: https://synbiohub.org/public/igem/BBa_K676002/annotation2131558/range2131558/1
component: BBa_E0432

https://synbiohub.org/public/igem/BBa_K676002/annotation2131558/range2131558/1
type: Range
persistent identity: range2131558
display id: range2131558
version: 1
direction: direction/reverse
top level: BBa_K676002
start: 98
end: 1014
orientation: inline

BBa_K239006
type: SequenceAnnotation
persistent identity: annotation2131544
display id: annotation2131544
version: 1
top level: BBa_K676002
location: https://synbiohub.org/public/igem/BBa_K676002/annotation2131544/range2131544/1
component: BBa_K239006

https://synbiohub.org/public/igem/BBa_K676002/annotation2131544/range2131544/1
type: Range
persistent identity: range2131544
display id: range2131544
version: 1
direction: direction/reverse
top level: BBa_K676002
start: 1
end: 89
orientation: inline

BBa_K364310
type: ComponentDefinition
persistent identity: BBa K364310
display id: BBa_K364310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EcR LBD
creator: Lior Malka
created: 2010-09-28T11:00:00Z
modified: 2015-05-08T01:12:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _486_
m user id: 0
owner id: 6778
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The ecdysone receptor is a nuclear receptor found in arthropods, where it controls development and contributes to other processes such as reproduction. The receptor is a non-covalent heterodimer of two proteins, the EcR protein and ultraspiracle protein (USP). It binds to and is activated by ecdysteroids. Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes,WHICH Ultimately causes physiological changes that result in ecdysis (moulting).
mutable notes: The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25
mutable provenance: Human EcR (of the nuclear hormone receptor superfamily)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K364310_sequence/1

BBa_I746916
type: ComponentDefinition
persistent identity: BBa I746916
display id: BBa_I746916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: superfolder GFP coding sequence
creator: Stefan Milde
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven).
mutable notes: Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis.
mutable provenance: Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: superfolder GFP coding region
sequence: https://synbiohub.org/public/igem/BBa_I746916_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1977535
display id: annotation1977535
version: 1
top level: BBa_I746916
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1

https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
type: Range
persistent identity: range1977535
display id: range1977535
version: 1
direction: direction/forward
top level: BBa_I746916
start: 715
end: 720
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1977533
display id: annotation1977533
version: 1
top level: BBa_I746916
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1

https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
type: Range
persistent identity: range1977533
display id: range1977533
version: 1
direction: direction/forward
top level: BBa_I746916
start: 1
end: 3
orientation: inline

superfolder GFP coding region
type: SequenceAnnotation
persistent identity: annotation1977534
display id: annotation1977534
version: 1
top level: BBa_I746916
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1

https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
type: Range
persistent identity: range1977534
display id: range1977534
version: 1
direction: direction/forward
top level: BBa_I746916
start: 1
end: 720
orientation: inline

BBa_K1175006
type: ComponentDefinition
persistent identity: BBa K1175006
display id: BBa_K1175006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: endo-beta-1,3-1,4 glucanase (BglS) from Bacillus Subtilis 168
creator: Nicholas K. Goldner
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
group u list: _1488_
m user id: 0
owner id: 14098
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BglS This gene encodes an endo-beta-1,3-1,4-glucanase (bglS), which is from the bacterium Bacillus subtilis subtilis 168. The enzyme will hydrolyze and thereby cleave internal 1,4 linkages adjacent to 1,3 linkages. Usage and Biology The substrates vulnerable to the bglS encoded enzyme are mixed linked beta-glucans. These glucans would have 1,3 and 1,4 beta linkages within the polysaccharide linking together the glucose monomers. Examples of these glucans can be found in oats, maize, and barley. (1) http://mic.sgmjournals.org/content/141/2/281.long (2) http://onlinelibrary.wiley.com/doi/10.1002/j.2050-0416.1984.tb04259.x/pdf
mutable notes: No design constraints were needed for RF10 insertion, a second stop codon was also added to the end of the coding region.
mutable provenance: Bacillus Subtilis 168
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: coding region
sequence annotation: Start Codon
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K1175006_sequence/1

coding region
type: SequenceAnnotation
persistent identity: annotation2350684
display id: annotation2350684
version: 1
top level: BBa_K1175006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1175006/annotation2350684/range2350684/1

https://synbiohub.org/public/igem/BBa_K1175006/annotation2350684/range2350684/1
type: Range
persistent identity: range2350684
display id: range2350684
version: 1
direction: direction/forward
top level: BBa_K1175006
start: 4
end: 726
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2350683
display id: annotation2350683
version: 1
top level: BBa_K1175006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1175006/annotation2350683/range2350683/1

https://synbiohub.org/public/igem/BBa_K1175006/annotation2350683/range2350683/1
type: Range
persistent identity: range2350683
display id: range2350683
version: 1
direction: direction/forward
top level: BBa_K1175006
start: 1
end: 3
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2350746
display id: annotation2350746
version: 1
top level: BBa_K1175006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1175006/annotation2350746/range2350746/1

https://synbiohub.org/public/igem/BBa_K1175006/annotation2350746/range2350746/1
type: Range
persistent identity: range2350746
display id: range2350746
version: 1
direction: direction/forward
top level: BBa_K1175006
start: 727
end: 732
orientation: inline

BBa_K779312
type: ComponentDefinition
persistent identity: BBa K779312
display id: BBa_K779312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mKate-HH - Hammerhead attached behind mKate MammoBlock
creator: Katie Bodner
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1033_
m user id: 0
owner id: 12603
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to be done by MIT iGEM 2012
mutable notes: to be done
mutable provenance: to be done
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mKate
sequence annotation: Spacer
sequence annotation: N117 Hammerhead
sequence: https://synbiohub.org/public/igem/BBa_K779312_sequence/1

mKate
type: SequenceAnnotation
persistent identity: annotation2204049
display id: annotation2204049
version: 1
top level: BBa_K779312
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K779312/annotation2204049/range2204049/1

https://synbiohub.org/public/igem/BBa_K779312/annotation2204049/range2204049/1
type: Range
persistent identity: range2204049
display id: range2204049
version: 1
direction: direction/forward
top level: BBa_K779312
start: 1
end: 718
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2204054
display id: annotation2204054
version: 1
top level: BBa_K779312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779312/annotation2204054/range2204054/1

https://synbiohub.org/public/igem/BBa_K779312/annotation2204054/range2204054/1
type: Range
persistent identity: range2204054
display id: range2204054
version: 1
direction: direction/forward
top level: BBa_K779312
start: 719
end: 722
orientation: inline

N117 Hammerhead
type: SequenceAnnotation
persistent identity: annotation2204063
display id: annotation2204063
version: 1
top level: BBa_K779312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779312/annotation2204063/range2204063/1

https://synbiohub.org/public/igem/BBa_K779312/annotation2204063/range2204063/1
type: Range
persistent identity: range2204063
display id: range2204063
version: 1
direction: direction/forward
top level: BBa_K779312
start: 723
end: 804
orientation: inline

BBa_K914007
type: ComponentDefinition
persistent identity: BBa K914007
display id: BBa_K914007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Meganuclease I-SceI controlled by pBad
creator: Denis Samuylov
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1179_
m user id: 0
owner id: 13487
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: I-SceI homing endonuclease expression is controlled by pBad promoter. Expression is induced with IPTG if LacI is present in the cell. I-SceI doesn't have an LVA degradation tag.
mutable notes: The system is leaky and meganuclease cut RS with high efficiency even without pBad induction.
mutable provenance: Promoter pBad and RBS was taken from an iGEM distribution plate (BBa_I13453 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_K914006
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_B0032
sequence annotation: BBa_K914006
sequence: https://synbiohub.org/public/igem/BBa_K914007_sequence/1

BBa_B0032
type: Component
persistent identity: component2188810
display id: component2188810
version: 1
top level: BBa_K914007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K914006
type: Component
persistent identity: component2188813
display id: component2188813
version: 1
top level: BBa_K914007
access: public
definition: https://synbiohub.org/public/igem/BBa_K914006/1

BBa_I13453
type: Component
persistent identity: component2188808
display id: component2188808
version: 1
top level: BBa_K914007
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2188808
display id: annotation2188808
version: 1
top level: BBa_K914007
location: https://synbiohub.org/public/igem/BBa_K914007/annotation2188808/range2188808/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K914007/annotation2188808/range2188808/1
type: Range
persistent identity: range2188808
display id: range2188808
version: 1
direction: direction/reverse
top level: BBa_K914007
start: 1
end: 130
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2188810
display id: annotation2188810
version: 1
top level: BBa_K914007
location: https://synbiohub.org/public/igem/BBa_K914007/annotation2188810/range2188810/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K914007/annotation2188810/range2188810/1
type: Range
persistent identity: range2188810
display id: range2188810
version: 1
direction: direction/forward
top level: BBa_K914007
start: 139
end: 151
orientation: inline

BBa_K914006
type: SequenceAnnotation
persistent identity: annotation2188813
display id: annotation2188813
version: 1
top level: BBa_K914007
location: https://synbiohub.org/public/igem/BBa_K914007/annotation2188813/range2188813/1
component: BBa_K914006

https://synbiohub.org/public/igem/BBa_K914007/annotation2188813/range2188813/1
type: Range
persistent identity: range2188813
display id: range2188813
version: 1
direction: direction/reverse
top level: BBa_K914007
start: 158
end: 869
orientation: inline

BBa_K750104
type: ComponentDefinition
persistent identity: BBa K750104
display id: BBa_K750104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBADcIT-1.0:cI protein expression system activated by arabinose(RBS strength:1.0)
creator: Sifan Wang,Yizhen Yan,Jianzhao Chi,Qingshu Wu
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1001_
m user id: 0
owner id: 14212
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: editing
mutable notes: editing
mutable provenance: editing
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K206000
component: BBa_B0015
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K750104_sequence/1

BBa_K206000
type: Component
persistent identity: component2259527
display id: component2259527
version: 1
top level: BBa_K750104
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_B0015
type: Component
persistent identity: component2259540
display id: component2259540
version: 1
top level: BBa_K750104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0051
type: Component
persistent identity: component2259533
display id: component2259533
version: 1
top level: BBa_K750104
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component2259529
display id: component2259529
version: 1
top level: BBa_K750104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2259533
display id: annotation2259533
version: 1
top level: BBa_K750104
location: https://synbiohub.org/public/igem/BBa_K750104/annotation2259533/range2259533/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K750104/annotation2259533/range2259533/1
type: Range
persistent identity: range2259533
display id: range2259533
version: 1
direction: direction/reverse
top level: BBa_K750104
start: 157
end: 931
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259540
display id: annotation2259540
version: 1
top level: BBa_K750104
location: https://synbiohub.org/public/igem/BBa_K750104/annotation2259540/range2259540/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K750104/annotation2259540/range2259540/1
type: Range
persistent identity: range2259540
display id: range2259540
version: 1
direction: direction/reverse
top level: BBa_K750104
start: 940
end: 1068
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2259529
display id: annotation2259529
version: 1
top level: BBa_K750104
location: https://synbiohub.org/public/igem/BBa_K750104/annotation2259529/range2259529/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K750104/annotation2259529/range2259529/1
type: Range
persistent identity: range2259529
display id: range2259529
version: 1
direction: direction/reverse
top level: BBa_K750104
start: 139
end: 150
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2259527
display id: annotation2259527
version: 1
top level: BBa_K750104
location: https://synbiohub.org/public/igem/BBa_K750104/annotation2259527/range2259527/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K750104/annotation2259527/range2259527/1
type: Range
persistent identity: range2259527
display id: range2259527
version: 1
direction: direction/reverse
top level: BBa_K750104
start: 1
end: 130
orientation: inline

BBa_K777000
type: ComponentDefinition
persistent identity: BBa K777000
display id: BBa_K777000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor
creator: Bianca Genenncher
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: The transmembrane receptor Tar of E. coli mediates chemotaxis towards aspartate.
mutable notes: One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
mutable provenance: The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B chromosome (NC_010473.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: TGA
sequence annotation: Tar coding region
sequence: https://synbiohub.org/public/igem/BBa_K777000_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2182659
display id: annotation2182659
version: 1
top level: BBa_K777000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182659/range2182659/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182659/range2182659/1
type: Range
persistent identity: range2182659
display id: range2182659
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1
end: 3
orientation: inline

TGA
type: SequenceAnnotation
persistent identity: annotation2182661
display id: annotation2182661
version: 1
top level: BBa_K777000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182661/range2182661/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182661/range2182661/1
type: Range
persistent identity: range2182661
display id: range2182661
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1660
end: 1662
orientation: inline

Tar coding region
type: SequenceAnnotation
persistent identity: annotation2182660
display id: annotation2182660
version: 1
top level: BBa_K777000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182660/range2182660/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182660/range2182660/1
type: Range
persistent identity: range2182660
display id: range2182660
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1
end: 1662
orientation: inline

ARL2A0104
type: ComponentDefinition
persistent identity: BBa I732108
display id: BBa_I732108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0104
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732108_sequence/1

LRLd
type: ComponentDefinition
persistent identity: BBa I732104
display id: BBa_I732104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLd
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732104_sequence/1

BBa_K1031302
type: ComponentDefinition
persistent identity: BBa K1031302
display id: BBa_K1031302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc-B0032-sfGFP-Terminator (HbpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-32-sfGFP-TT (HbpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: BBa_B0015
sequence annotation: Ph
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1031302_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360438
display id: annotation2360438
version: 1
top level: BBa_K1031302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1
type: Range
persistent identity: range2360438
display id: range2360438
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 254
end: 976
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360439
display id: annotation2360439
version: 1
top level: BBa_K1031302
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1
type: Range
persistent identity: range2360439
display id: range2360439
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 983
end: 1111
orientation: inline

Ph
type: SequenceAnnotation
persistent identity: annotation2360436
display id: annotation2360436
version: 1
top level: BBa_K1031302
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1
type: Range
persistent identity: range2360436
display id: range2360436
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 1
end: 229
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2360437
display id: annotation2360437
version: 1
top level: BBa_K1031302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1
type: Range
persistent identity: range2360437
display id: range2360437
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 236
end: 247
orientation: inline

BBa_K274391
type: ComponentDefinition
persistent identity: BBa K274391
display id: BBa_K274391
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> PhiR73 pag - PO promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_I746361
component: BBa_I746352
sequence annotation: BBa_I746352
sequence annotation: BBa_B0012
sequence annotation: BBa_I746361
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K274391_sequence/1

BBa_B0012
type: Component
persistent identity: component2055382
display id: component2055382
version: 1
top level: BBa_K274391
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2055380
display id: component2055380
version: 1
top level: BBa_K274391
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746361
type: Component
persistent identity: component2055387
display id: component2055387
version: 1
top level: BBa_K274391
access: public
definition: https://synbiohub.org/public/igem/BBa_I746361/1

BBa_I746352
type: Component
persistent identity: component2055379
display id: component2055379
version: 1
top level: BBa_K274391
access: public
definition: https://synbiohub.org/public/igem/BBa_I746352/1

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2055379
display id: annotation2055379
version: 1
top level: BBa_K274391
location: https://synbiohub.org/public/igem/BBa_K274391/annotation2055379/range2055379/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K274391/annotation2055379/range2055379/1
type: Range
persistent identity: range2055379
display id: range2055379
version: 1
direction: direction/reverse
top level: BBa_K274391
start: 1
end: 264
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055382
display id: annotation2055382
version: 1
top level: BBa_K274391
location: https://synbiohub.org/public/igem/BBa_K274391/annotation2055382/range2055382/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274391/annotation2055382/range2055382/1
type: Range
persistent identity: range2055382
display id: range2055382
version: 1
direction: direction/forward
top level: BBa_K274391
start: 361
end: 401
orientation: inline

BBa_I746361
type: SequenceAnnotation
persistent identity: annotation2055387
display id: annotation2055387
version: 1
top level: BBa_K274391
location: https://synbiohub.org/public/igem/BBa_K274391/annotation2055387/range2055387/1
component: BBa_I746361

https://synbiohub.org/public/igem/BBa_K274391/annotation2055387/range2055387/1
type: Range
persistent identity: range2055387
display id: range2055387
version: 1
direction: direction/reverse
top level: BBa_K274391
start: 410
end: 501
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055380
display id: annotation2055380
version: 1
top level: BBa_K274391
location: https://synbiohub.org/public/igem/BBa_K274391/annotation2055380/range2055380/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274391/annotation2055380/range2055380/1
type: Range
persistent identity: range2055380
display id: range2055380
version: 1
direction: direction/forward
top level: BBa_K274391
start: 273
end: 352
orientation: inline

BBa_K249002
type: ComponentDefinition
persistent identity: BBa K249002
display id: BBa_K249002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lumazine Synthase
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _242_342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lumazine Synthase is an enzyme which creates Lumazine, a product which aggregates forming a hollow spheroid which can act as a mirocompartment, or artificial organelle. The Lumazine forms negatively charged pores, which can be used to introduce proteins. The proteins which are being introduced into the microcompartment must be equipped with an Arginine Tag.
mutable notes: point mutations of EcoRI and PstI sites?
mutable provenance: A Science Paper?
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K249002_sequence/1

BBa_K953004
type: ComponentDefinition
persistent identity: BBa K953004
display id: BBa_K953004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hemeoxygenase with RBS (E. coli Codon Optimised)
creator: David Dubow
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1221_
m user id: 0
owner id: 10455
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
mutable notes: Codon optimisation for E. coli, whilst staying within the GC constraints of gibson assembly.
mutable provenance: Gibson assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence annotation: Heme Oxygenase
sequence: https://synbiohub.org/public/igem/BBa_K953004_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2199030
display id: annotation2199030
version: 1
top level: BBa_K953004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K953004/annotation2199030/range2199030/1

https://synbiohub.org/public/igem/BBa_K953004/annotation2199030/range2199030/1
type: Range
persistent identity: range2199030
display id: range2199030
version: 1
direction: direction/forward
top level: BBa_K953004
start: 1
end: 21
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2199031
display id: annotation2199031
version: 1
top level: BBa_K953004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K953004/annotation2199031/range2199031/1

https://synbiohub.org/public/igem/BBa_K953004/annotation2199031/range2199031/1
type: Range
persistent identity: range2199031
display id: range2199031
version: 1
direction: direction/forward
top level: BBa_K953004
start: 23
end: 25
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2199033
display id: annotation2199033
version: 1
top level: BBa_K953004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K953004/annotation2199033/range2199033/1

https://synbiohub.org/public/igem/BBa_K953004/annotation2199033/range2199033/1
type: Range
persistent identity: range2199033
display id: range2199033
version: 1
direction: direction/forward
top level: BBa_K953004
start: 743
end: 748
orientation: inline

Heme Oxygenase
type: SequenceAnnotation
persistent identity: annotation2199032
display id: annotation2199032
version: 1
top level: BBa_K953004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K953004/annotation2199032/range2199032/1

https://synbiohub.org/public/igem/BBa_K953004/annotation2199032/range2199032/1
type: Range
persistent identity: range2199032
display id: range2199032
version: 1
direction: direction/forward
top level: BBa_K953004
start: 26
end: 742
orientation: inline

BBa_K274395
type: ComponentDefinition
persistent identity: BBa K274395
display id: BBa_K274395
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> PhiR73 pag - PLL promoter -> PoPS
creator: Vivian Mullin
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: n/a
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746352
component: BBa_I746365
component: BBa_B0015
sequence annotation: BBa_I746365
sequence annotation: BBa_B0015
sequence annotation: BBa_I746352
sequence: https://synbiohub.org/public/igem/BBa_K274395_sequence/1

BBa_I746352
type: Component
persistent identity: component2373182
display id: component2373182
version: 1
top level: BBa_K274395
access: public
definition: https://synbiohub.org/public/igem/BBa_I746352/1

BBa_I746365
type: Component
persistent identity: component2373191
display id: component2373191
version: 1
top level: BBa_K274395
access: public
definition: https://synbiohub.org/public/igem/BBa_I746365/1

BBa_B0015
type: Component
persistent identity: component2373189
display id: component2373189
version: 1
top level: BBa_K274395
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I746365
type: SequenceAnnotation
persistent identity: annotation2373191
display id: annotation2373191
version: 1
top level: BBa_K274395
location: https://synbiohub.org/public/igem/BBa_K274395/annotation2373191/range2373191/1
component: BBa_I746365

https://synbiohub.org/public/igem/BBa_K274395/annotation2373191/range2373191/1
type: Range
persistent identity: range2373191
display id: range2373191
version: 1
direction: direction/reverse
top level: BBa_K274395
start: 410
end: 501
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2373189
display id: annotation2373189
version: 1
top level: BBa_K274395
location: https://synbiohub.org/public/igem/BBa_K274395/annotation2373189/range2373189/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K274395/annotation2373189/range2373189/1
type: Range
persistent identity: range2373189
display id: range2373189
version: 1
direction: direction/reverse
top level: BBa_K274395
start: 273
end: 401
orientation: inline

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2373182
display id: annotation2373182
version: 1
top level: BBa_K274395
location: https://synbiohub.org/public/igem/BBa_K274395/annotation2373182/range2373182/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K274395/annotation2373182/range2373182/1
type: Range
persistent identity: range2373182
display id: range2373182
version: 1
direction: direction/reverse
top level: BBa_K274395
start: 1
end: 264
orientation: inline

BBa_K1051502
type: ComponentDefinition
persistent identity: BBa K1051502
display id: BBa_K1051502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sic1 mutation, non stop codon
creator: Lin Qiongfen
created: 2013-08-05T11:00:00Z
modified: 2015-05-08T01:08:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1358_
m user id: 0
owner id: 15918
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the mutation of Sic1, we mainly mutate the polyphosphoric acid sites of the Sic1. Here we just use it as a cell cycle regulate switch, it may let the cell cycle stop at G1 phase.
mutable notes: Mutate the polyphosphoric acid sites of the Sic1, at least seven sites of it.
mutable provenance: It come from saccharomyces cerevisiae's whole genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1051502_sequence/1

BBa_K318514
type: ComponentDefinition
persistent identity: BBa K318514
display id: BBa_K318514
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: acrRA
creator: Sarah R. Sandock
created: 2010-09-25T11:00:00Z
modified: 2015-05-08T01:11:58Z
discontinued: false
dominant: false
group u list: _438_
m user id: 0
owner id: 4504
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: uc
mutable notes: uc
mutable provenance: uc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K318514_sequence/1

BBa_K216006
type: ComponentDefinition
persistent identity: BBa K216006
display id: BBa_K216006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pentaerythritol tetranitrate reductase with native rbs
creator: Edinburgh iGEM 2009
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for the enzyme pentaerythritol tetranitrate reductase (PETN reductase) of ''Enterobacter cloacae'' PB2. This enzyme reductively denitrates nitrate esters such as PETN (pentaerythritol tetranitrate, penthrite) GTN (glycerol trinitrate, nitroglycerine) and EGDN (ethylene glycol dinitrate) yielding the alcohol and nitrite ion. It can also reductively attack nitroaromatic compounds such as TNT (2,4,6-trinitrotoluene) forming initially hydride-Meisenheimer complexes which break down via an unknown route to yield nitrite and non-aromatic products. Reference: Binks, P.R., French, C.E., Nicklin, S., & Bruce, N.C. (1996). Degradation of pentaerythritol tetranitrate by ''Enterobacter cloacae'' PB2. Applied and Environmental Microbiology 62(4), 1214-1219. Reference 2: French,C.E., Nicklin,S. and Bruce,N.C. 1996. Sequence and properties of pentaerythritol tetranitrate reductase from ''Enterobacter cloacae'' PB2. J. Bacteriol. 178 (22), 6623-6627 (1996).
mutable notes: No special considerations, but due to an error, the primers were actually designed based on the homologous gene of another strain of ''Enterobacter cloacae'', which has a very similar coding sequence but different sequence in the upstream region. The sequence shown here is the correct sequence determined by sequencing the construct.
mutable provenance: This part was amplified from plasmid pONR1 which bears the PETN reductase gene originally cloned from ''Enterobacter cloacae'' PB2.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: stop
sequence annotation: RBS
sequence annotation: PETN reductase coding region
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K216006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2046803
display id: annotation2046803
version: 1
top level: BBa_K216006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K216006/annotation2046803/range2046803/1

https://synbiohub.org/public/igem/BBa_K216006/annotation2046803/range2046803/1
type: Range
persistent identity: range2046803
display id: range2046803
version: 1
direction: direction/forward
top level: BBa_K216006
start: 1104
end: 1109
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2046801
display id: annotation2046801
version: 1
top level: BBa_K216006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K216006/annotation2046801/range2046801/1

https://synbiohub.org/public/igem/BBa_K216006/annotation2046801/range2046801/1
type: Range
persistent identity: range2046801
display id: range2046801
version: 1
direction: direction/forward
top level: BBa_K216006
start: 1
end: 3
orientation: inline

PETN reductase coding region
type: SequenceAnnotation
persistent identity: annotation2046804
display id: annotation2046804
version: 1
top level: BBa_K216006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K216006/annotation2046804/range2046804/1

https://synbiohub.org/public/igem/BBa_K216006/annotation2046804/range2046804/1
type: Range
persistent identity: range2046804
display id: range2046804
version: 1
direction: direction/forward
top level: BBa_K216006
start: 9
end: 1106
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2046802
display id: annotation2046802
version: 1
top level: BBa_K216006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K216006/annotation2046802/range2046802/1

https://synbiohub.org/public/igem/BBa_K216006/annotation2046802/range2046802/1
type: Range
persistent identity: range2046802
display id: range2046802
version: 1
direction: direction/forward
top level: BBa_K216006
start: 9
end: 11
orientation: inline

BBa_J97000
type: ComponentDefinition
persistent identity: BBa J97000
display id: BBa_J97000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IP-Free EiraCFP (Cyan Fluorescent Protein)
creator: Drew Endy
created: 2014-02-04T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement. Excitation 398nm, Emission 498nm Molecular Weight: 26.0kDA, Length: 233 amino acids
mutable notes: DNA 2.0 have created a large set of new colored and fluorescent proteins by back-translating a set of sequences from Genbank, using their proprietary GeneDesigner software. To create these proteins, they simultaneously minimized sequence differences and and derived an E. coli codon bias for high protein expression. Gene fragments were designed to create chimeric genes when pooled. A subset of the resultant chimeras were selected as base genes from which multiple iterations of libraries with targeted substitutions were made. (from DNA 2.0)
mutable provenance: Non-Aequorea based sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97000_sequence/1

BBa_K957000
type: ComponentDefinition
persistent identity: BBa K957000
display id: BBa_K957000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arsenic inducible mtrB with cut sites flanking RBS
creator: Dylan Patrick Webster
created: 2012-07-25T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1228_
m user id: 0
owner id: 13504
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The purpose of this part is to upregulate mtrB expression in response to arsenic. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to arsenic. Within the genetic circuit, arsR acts as a negative autoregulator, repressing not only the expression of downstream mtrB, but also its own expression. Upon association with arsenic salts, ArsR dissociates from the operator of the arsenic inducible promoter, upregulating expression of downstream protein. Because mtrB activity is requisite for functionality of the mtr electron pathway in Shewanella spp., arsenic thus induces electron shuttling through the mtr pathway.
mutable notes: Primers were designed such that the wild-type Shine Dalgarno sequence was included upstream of the mtrB start codon, maintaining spacing. However, the appended BamHI cutsite downstream of the Shine Dalgarno sequence may alter translational efficiency.
mutable provenance: Arsenic-sensitive component of this composite part was originally derived from the arsenic detoxification operon of E. coli strain JM109; BioBrick part BBa_J33201 was the source of DNA. mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: mtrB
sequence annotation: -35 sequence
sequence annotation: rbs
sequence annotation: mtrB rbs
sequence annotation: BamHI cut site
sequence annotation: arsR
sequence annotation: AscI cut site
sequence annotation: ArsR binding site
sequence annotation: BBa_J33201
sequence annotation: BBa_K098989
sequence annotation: KpnI cut site
sequence annotation: -10 sequence
sequence: https://synbiohub.org/public/igem/BBa_K957000_sequence/1

mtrB
type: SequenceAnnotation
persistent identity: annotation2178743
display id: annotation2178743
version: 1
top level: BBa_K957000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178743/range2178743/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178743/range2178743/1
type: Range
persistent identity: range2178743
display id: range2178743
version: 1
direction: direction/forward
top level: BBa_K957000
start: 497
end: 2590
orientation: inline

-35 sequence
type: SequenceAnnotation
persistent identity: annotation2178736
display id: annotation2178736
version: 1
top level: BBa_K957000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178736/range2178736/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178736/range2178736/1
type: Range
persistent identity: range2178736
display id: range2178736
version: 1
direction: direction/forward
top level: BBa_K957000
start: 57
end: 62
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2178738
display id: annotation2178738
version: 1
top level: BBa_K957000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178738/range2178738/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178738/range2178738/1
type: Range
persistent identity: range2178738
display id: range2178738
version: 1
direction: direction/forward
top level: BBa_K957000
start: 108
end: 112
orientation: inline

mtrB rbs
type: SequenceAnnotation
persistent identity: annotation2178741
display id: annotation2178741
version: 1
top level: BBa_K957000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178741/range2178741/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178741/range2178741/1
type: Range
persistent identity: range2178741
display id: range2178741
version: 1
direction: direction/forward
top level: BBa_K957000
start: 484
end: 489
orientation: inline

BamHI cut site
type: SequenceAnnotation
persistent identity: annotation2178742
display id: annotation2178742
version: 1
top level: BBa_K957000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178742/range2178742/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178742/range2178742/1
type: Range
persistent identity: range2178742
display id: range2178742
version: 1
direction: direction/forward
top level: BBa_K957000
start: 491
end: 496
orientation: inline

arsR
type: SequenceAnnotation
persistent identity: annotation2178739
display id: annotation2178739
version: 1
top level: BBa_K957000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178739/range2178739/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178739/range2178739/1
type: Range
persistent identity: range2178739
display id: range2178739
version: 1
direction: direction/forward
top level: BBa_K957000
start: 119
end: 472
orientation: inline

AscI cut site
type: SequenceAnnotation
persistent identity: annotation2178740
display id: annotation2178740
version: 1
top level: BBa_K957000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178740/range2178740/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178740/range2178740/1
type: Range
persistent identity: range2178740
display id: range2178740
version: 1
direction: direction/forward
top level: BBa_K957000
start: 474
end: 481
orientation: inline

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation2178735
display id: annotation2178735
version: 1
top level: BBa_K957000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178735/range2178735/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178735/range2178735/1
type: Range
persistent identity: range2178735
display id: range2178735
version: 1
direction: direction/forward
top level: BBa_K957000
start: 36
end: 54
orientation: inline

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation2178734
display id: annotation2178734
version: 1
top level: BBa_K957000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178734/range2178734/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178734/range2178734/1
type: Range
persistent identity: range2178734
display id: range2178734
version: 1
direction: direction/forward
top level: BBa_K957000
start: 1
end: 472
orientation: inline

BBa_K098989
type: SequenceAnnotation
persistent identity: annotation2178744
display id: annotation2178744
version: 1
top level: BBa_K957000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178744/range2178744/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178744/range2178744/1
type: Range
persistent identity: range2178744
display id: range2178744
version: 1
direction: direction/forward
top level: BBa_K957000
start: 497
end: 2590
orientation: inline

KpnI cut site
type: SequenceAnnotation
persistent identity: annotation2179155
display id: annotation2179155
version: 1
top level: BBa_K957000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2179155/range2179155/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2179155/range2179155/1
type: Range
persistent identity: range2179155
display id: range2179155
version: 1
direction: direction/forward
top level: BBa_K957000
start: 2620
end: 2625
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation2178737
display id: annotation2178737
version: 1
top level: BBa_K957000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K957000/annotation2178737/range2178737/1

https://synbiohub.org/public/igem/BBa_K957000/annotation2178737/range2178737/1
type: Range
persistent identity: range2178737
display id: range2178737
version: 1
direction: direction/forward
top level: BBa_K957000
start: 80
end: 85
orientation: inline

BBa_K173003
type: ComponentDefinition
persistent identity: BBa K173003
display id: BBa_K173003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pdc and adhB protein generator
creator: Lorenzo Pasotti, Giulio Chiesa, Manuel Lupotto, Paolo Magni
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _281_
m user id: 0
owner id: 2583
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K173017
component: BBa_B0010
component: BBa_B0030
component: BBa_K173016
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_K173016
sequence annotation: BBa_K173017
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K173003_sequence/1

BBa_K173017
type: Component
persistent identity: component2044293
display id: component2044293
version: 1
top level: BBa_K173003
access: public
definition: https://synbiohub.org/public/igem/BBa_K173017/1

BBa_B0010
type: Component
persistent identity: component2044294
display id: component2044294
version: 1
top level: BBa_K173003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component2044291
display id: component2044291
version: 1
top level: BBa_K173003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: Component
persistent identity: component2044287
display id: component2044287
version: 1
top level: BBa_K173003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K173016
type: Component
persistent identity: component2044289
display id: component2044289
version: 1
top level: BBa_K173003
access: public
definition: https://synbiohub.org/public/igem/BBa_K173016/1

BBa_B0012
type: Component
persistent identity: component2044296
display id: component2044296
version: 1
top level: BBa_K173003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2044291
display id: annotation2044291
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044291/range2044291/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K173003/annotation2044291/range2044291/1
type: Range
persistent identity: range2044291
display id: range2044291
version: 1
direction: direction/forward
top level: BBa_K173003
start: 1740
end: 1754
orientation: inline

BBa_K173016
type: SequenceAnnotation
persistent identity: annotation2044289
display id: annotation2044289
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044289/range2044289/1
component: BBa_K173016

https://synbiohub.org/public/igem/BBa_K173003/annotation2044289/range2044289/1
type: Range
persistent identity: range2044289
display id: range2044289
version: 1
direction: direction/reverse
top level: BBa_K173003
start: 22
end: 1731
orientation: inline

BBa_K173017
type: SequenceAnnotation
persistent identity: annotation2044293
display id: annotation2044293
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044293/range2044293/1
component: BBa_K173017

https://synbiohub.org/public/igem/BBa_K173003/annotation2044293/range2044293/1
type: Range
persistent identity: range2044293
display id: range2044293
version: 1
direction: direction/reverse
top level: BBa_K173003
start: 1761
end: 2915
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2044287
display id: annotation2044287
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044287/range2044287/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K173003/annotation2044287/range2044287/1
type: Range
persistent identity: range2044287
display id: range2044287
version: 1
direction: direction/forward
top level: BBa_K173003
start: 1
end: 15
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2044296
display id: annotation2044296
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044296/range2044296/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K173003/annotation2044296/range2044296/1
type: Range
persistent identity: range2044296
display id: range2044296
version: 1
direction: direction/forward
top level: BBa_K173003
start: 3012
end: 3052
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2044294
display id: annotation2044294
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044294/range2044294/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K173003/annotation2044294/range2044294/1
type: Range
persistent identity: range2044294
display id: range2044294
version: 1
direction: direction/forward
top level: BBa_K173003
start: 2924
end: 3003
orientation: inline

BBa_K398206
type: ComponentDefinition
persistent identity: BBa K398206
display id: BBa_K398206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AlnA generator (IPTG inducible)
creator: Eva Brinkman
created: 2010-10-09T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: AlnA is a protein with emulsification activity originated from the natural oil degrading bacterium Acinetobacter radioresistens. It has been shown that this protein keeps its emulsifying capacitiesis when it is produced by other organisms, such as Escherichia coli. AlnA has an amphipathic structure, which allows it to form micelles that accumulate at the interface between liquids of different polarities such as water and oil. This process is based upon the ability of biosurfactants to reduce surface tension, blocking the formation of hydrogen bridges and certain hydrophilic and hydrophobic interactions. The emulsifier helps to disperse the oil by emulsifying the oil, thus increasing the surface area for the growth of microorganisms on hydrocarbons
mutable notes: none
mutable provenance: Accession no. AY033946
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K398200
component: BBa_B0012
component: BBa_R0011
component: BBa_B0032
component: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K398200
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K398206_sequence/1

BBa_K398200
type: Component
persistent identity: component2088282
display id: component2088282
version: 1
top level: BBa_K398206
access: public
definition: https://synbiohub.org/public/igem/BBa_K398200/1

BBa_B0012
type: Component
persistent identity: component2088285
display id: component2088285
version: 1
top level: BBa_K398206
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component2088271
display id: component2088271
version: 1
top level: BBa_K398206
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0032
type: Component
persistent identity: component2088277
display id: component2088277
version: 1
top level: BBa_K398206
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component2088283
display id: component2088283
version: 1
top level: BBa_K398206
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2088271
display id: annotation2088271
version: 1
top level: BBa_K398206
location: https://synbiohub.org/public/igem/BBa_K398206/annotation2088271/range2088271/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K398206/annotation2088271/range2088271/1
type: Range
persistent identity: range2088271
display id: range2088271
version: 1
direction: direction/forward
top level: BBa_K398206
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2088283
display id: annotation2088283
version: 1
top level: BBa_K398206
location: https://synbiohub.org/public/igem/BBa_K398206/annotation2088283/range2088283/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K398206/annotation2088283/range2088283/1
type: Range
persistent identity: range2088283
display id: range2088283
version: 1
direction: direction/forward
top level: BBa_K398206
start: 1138
end: 1217
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2088285
display id: annotation2088285
version: 1
top level: BBa_K398206
location: https://synbiohub.org/public/igem/BBa_K398206/annotation2088285/range2088285/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K398206/annotation2088285/range2088285/1
type: Range
persistent identity: range2088285
display id: range2088285
version: 1
direction: direction/forward
top level: BBa_K398206
start: 1226
end: 1266
orientation: inline

BBa_K398200
type: SequenceAnnotation
persistent identity: annotation2088282
display id: annotation2088282
version: 1
top level: BBa_K398206
location: https://synbiohub.org/public/igem/BBa_K398206/annotation2088282/range2088282/1
component: BBa_K398200

https://synbiohub.org/public/igem/BBa_K398206/annotation2088282/range2088282/1
type: Range
persistent identity: range2088282
display id: range2088282
version: 1
direction: direction/reverse
top level: BBa_K398206
start: 83
end: 1129
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2088277
display id: annotation2088277
version: 1
top level: BBa_K398206
location: https://synbiohub.org/public/igem/BBa_K398206/annotation2088277/range2088277/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K398206/annotation2088277/range2088277/1
type: Range
persistent identity: range2088277
display id: range2088277
version: 1
direction: direction/forward
top level: BBa_K398206
start: 64
end: 76
orientation: inline

CmR-T
type: ComponentDefinition
persistent identity: BBa K143064
display id: BBa_K143064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chloramphenicol resistance protein - Terminator
creator: Chris Hirst
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Chloraphemicol acetyltransferase protein( BBa_J31005 ) coupled to the double terminator ( BBa_B0015 ). Chloraphemicol acetyltransferase confers resistance to Chloraphemicol. The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012 . The double terminator allows the CAT to be incorporated into a closed transcriptional unit.
mutable notes: Chloraphemicol acetyltransferase is an exisiting registry protein. The double terminator is the most commonly used registry termiantor.
mutable provenance: The Chloraphemicol acetyltransferase and double terminator were taken both taken from the registry.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_J31005
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J31005
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143064_sequence/1

BBa_B0010
type: Component
persistent identity: component1980004
display id: component1980004
version: 1
top level: CmR-T
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J31005
type: Component
persistent identity: component1980003
display id: component1980003
version: 1
top level: CmR-T
access: public
definition: https://synbiohub.org/public/igem/BBa_J31005/1

BBa_B0012
type: Component
persistent identity: component1980006
display id: component1980006
version: 1
top level: CmR-T
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1980004
display id: annotation1980004
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980004/range1980004/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143064/annotation1980004/range1980004/1
type: Range
persistent identity: range1980004
display id: range1980004
version: 1
direction: direction/forward
top level: CmR-T
start: 669
end: 748
orientation: inline

BBa_J31005
type: SequenceAnnotation
persistent identity: annotation1980003
display id: annotation1980003
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980003/range1980003/1
component: BBa_J31005

https://synbiohub.org/public/igem/BBa_K143064/annotation1980003/range1980003/1
type: Range
persistent identity: range1980003
display id: range1980003
version: 1
direction: direction/reverse
top level: CmR-T
start: 1
end: 660
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1980006
display id: annotation1980006
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980006/range1980006/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143064/annotation1980006/range1980006/1
type: Range
persistent identity: range1980006
display id: range1980006
version: 1
direction: direction/forward
top level: CmR-T
start: 757
end: 797
orientation: inline

BBa_K779311
type: ComponentDefinition
persistent identity: BBa K779311
display id: BBa_K779311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HH-mKate - Hammerhead attached to front of mKate MammoBlock
creator: Katie Bodner
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1033_
m user id: 0
owner id: 12603
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be filled out by MIT iGEM 2012
mutable notes: to be filled out
mutable provenance: To be filled out by MIT iGEM 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Spacer
sequence annotation: Hammerhead N117
sequence annotation: Kozak
sequence annotation: mKate
sequence: https://synbiohub.org/public/igem/BBa_K779311_sequence/1

Spacer
type: SequenceAnnotation
persistent identity: annotation2203984
display id: annotation2203984
version: 1
top level: BBa_K779311
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779311/annotation2203984/range2203984/1

https://synbiohub.org/public/igem/BBa_K779311/annotation2203984/range2203984/1
type: Range
persistent identity: range2203984
display id: range2203984
version: 1
direction: direction/forward
top level: BBa_K779311
start: 84
end: 88
orientation: inline

Hammerhead N117
type: SequenceAnnotation
persistent identity: annotation2203982
display id: annotation2203982
version: 1
top level: BBa_K779311
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779311/annotation2203982/range2203982/1

https://synbiohub.org/public/igem/BBa_K779311/annotation2203982/range2203982/1
type: Range
persistent identity: range2203982
display id: range2203982
version: 1
direction: direction/forward
top level: BBa_K779311
start: 1
end: 83
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2203985
display id: annotation2203985
version: 1
top level: BBa_K779311
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K779311/annotation2203985/range2203985/1

https://synbiohub.org/public/igem/BBa_K779311/annotation2203985/range2203985/1
type: Range
persistent identity: range2203985
display id: range2203985
version: 1
direction: direction/forward
top level: BBa_K779311
start: 89
end: 94
orientation: inline

mKate
type: SequenceAnnotation
persistent identity: annotation2203999
display id: annotation2203999
version: 1
top level: BBa_K779311
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K779311/annotation2203999/range2203999/1

https://synbiohub.org/public/igem/BBa_K779311/annotation2203999/range2203999/1
type: Range
persistent identity: range2203999
display id: range2203999
version: 1
direction: direction/forward
top level: BBa_K779311
start: 95
end: 802
orientation: inline

BBa_K1185001
type: ComponentDefinition
persistent identity: BBa K1185001
display id: BBa_K1185001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HBsu-sfGFP
creator: Vincent Leonardo
created: 2013-08-28T11:00:00Z
modified: 2015-06-08T09:31:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
m user id: 4206
owner id: 16794
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick codes for a HBsu protein attached to a superfolded green fluorescent protein (sfGFP). HBsu is a non-specific DNA binding protein that binds to DNA as a homodimer. The HBsu is joined to the sfGFP through ten amino acid flexible linker sequence. This allows the observation of ''Bacillus subtilis'' DNA using fluorescence microscopy. The integration strategy that we opted for was to clone in the BioBrick into the Multiple cloning site of pMutin4 backbone. First we attached HindIII restriction sites on 5'end and SacII restriction sites on 3'end of the BioBrick. We then cut the pMutin4 backbone and BioBrick using the previously mentioned restriction enzymes, this allowed us to ligate the plasmid and BioBrick together. We also attached a ~300bp amyE homology region onto the 3'end of the BioBrick after the SacII to allow the single cross over and integration of the whole plasmid into the genome. The pMutin4 plasmid contains a ery+ resistance marker for ''B.subtilis'' and amp+ for ''E.coli'' and also contains lacI, lacZ and Pspac promoter which is an IPTG induced promoter which regulated the transcription of this BioBrick. An alternative method to use this part would be to clone this BioBrick out and use any Assembly protocol to attach a desired promoter, RBS and antibiotic resistance genes.
mutable notes: None
mutable provenance: The source of the HBsu coding sequence was the ''B. subtilis'' strain 168 genome, SwissProtTM accession number: P08821. The sfGFP sequence was sourced from BBa_E0040 and the linker from: BBa_K105012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP_BBa_I746916
sequence annotation: RBS-with post spacer
sequence annotation: linker sequence BBa_K105012
sequence annotation: 3-frame stop codon
sequence annotation: hbs
sequence: https://synbiohub.org/public/igem/BBa_K1185001_sequence/1

sfGFP_BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2333335
display id: annotation2333335
version: 1
top level: BBa_K1185001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333335/range2333335/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333335/range2333335/1
type: Range
persistent identity: range2333335
display id: range2333335
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 327
end: 1046
orientation: inline

RBS-with post spacer
type: SequenceAnnotation
persistent identity: annotation2333331
display id: annotation2333331
version: 1
top level: BBa_K1185001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333331/range2333331/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333331/range2333331/1
type: Range
persistent identity: range2333331
display id: range2333331
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 1
end: 20
orientation: inline

linker sequence BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2333334
display id: annotation2333334
version: 1
top level: BBa_K1185001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333334/range2333334/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333334/range2333334/1
type: Range
persistent identity: range2333334
display id: range2333334
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 297
end: 326
orientation: inline

3-frame stop codon
type: SequenceAnnotation
persistent identity: annotation2333336
display id: annotation2333336
version: 1
top level: BBa_K1185001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333336/range2333336/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333336/range2333336/1
type: Range
persistent identity: range2333336
display id: range2333336
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 1047
end: 1054
orientation: inline

hbs
type: SequenceAnnotation
persistent identity: annotation2333332
display id: annotation2333332
version: 1
top level: BBa_K1185001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333332/range2333332/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333332/range2333332/1
type: Range
persistent identity: range2333332
display id: range2333332
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 21
end: 296
orientation: inline

BBa_K515005
type: ComponentDefinition
persistent identity: BBa K515005
display id: BBa_K515005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: superfolder GFP (sfGFP)
creator: Atipat Patharagulpong
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: a translational unit of superfolded GFP
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K515005_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2126852
display id: annotation2126852
version: 1
top level: BBa_K515005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K515005/annotation2126852/range2126852/1

https://synbiohub.org/public/igem/BBa_K515005/annotation2126852/range2126852/1
type: Range
persistent identity: range2126852
display id: range2126852
version: 1
direction: direction/forward
top level: BBa_K515005
start: 25
end: 744
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2126851
display id: annotation2126851
version: 1
top level: BBa_K515005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K515005/annotation2126851/range2126851/1

https://synbiohub.org/public/igem/BBa_K515005/annotation2126851/range2126851/1
type: Range
persistent identity: range2126851
display id: range2126851
version: 1
direction: direction/forward
top level: BBa_K515005
start: 1
end: 24
orientation: inline

BBa_M36042
type: ComponentDefinition
persistent identity: BBa M36042
display id: BBa_M36042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EfeU elemental iron (II) transporter
creator: Tom McLaughlin
created: 2011-04-30T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9545
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the reconstructed gene for the EfeU iron transporter. This gene is inactive in K-12 strain E. coli, but has been characterized in the Nissle 1917 strain. (Grobe, Scherer et al. PMID: 16987175.)
mutable notes: The sequence was conceptually reconstructed from the frame-shifted version. The Ecogene entry has an "N" for the nucleotide which is uncertain, but a comment in that entry says that the correct amino acid is likely glycine. Thus we chose N = G in the final sequence.
mutable provenance: From wild-type Nissle 1917 E. coli. The sequence came from the Ecogene entry EG13860.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: REGLE motif
sequence annotation: Start codon
sequence annotation: Stop codon
sequence annotation: Repaired frameshift (see notes)
sequence: https://synbiohub.org/public/igem/BBa_M36042_sequence/1

REGLE motif
type: SequenceAnnotation
persistent identity: annotation2119195
display id: annotation2119195
version: 1
top level: BBa_M36042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119195/range2119195/1

https://synbiohub.org/public/igem/BBa_M36042/annotation2119195/range2119195/1
type: Range
persistent identity: range2119195
display id: range2119195
version: 1
direction: direction/forward
top level: BBa_M36042
start: 385
end: 399
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2119094
display id: annotation2119094
version: 1
top level: BBa_M36042
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119094/range2119094/1

https://synbiohub.org/public/igem/BBa_M36042/annotation2119094/range2119094/1
type: Range
persistent identity: range2119094
display id: range2119094
version: 1
direction: direction/forward
top level: BBa_M36042
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2119197
display id: annotation2119197
version: 1
top level: BBa_M36042
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119197/range2119197/1

https://synbiohub.org/public/igem/BBa_M36042/annotation2119197/range2119197/1
type: Range
persistent identity: range2119197
display id: range2119197
version: 1
direction: direction/forward
top level: BBa_M36042
start: 829
end: 831
orientation: inline

REGLE motif
type: SequenceAnnotation
persistent identity: annotation2119177
display id: annotation2119177
version: 1
top level: BBa_M36042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119177/range2119177/1

https://synbiohub.org/public/igem/BBa_M36042/annotation2119177/range2119177/1
type: Range
persistent identity: range2119177
display id: range2119177
version: 1
direction: direction/forward
top level: BBa_M36042
start: 28
end: 42
orientation: inline

Repaired frameshift (see notes)
type: SequenceAnnotation
persistent identity: annotation2119198
display id: annotation2119198
version: 1
top level: BBa_M36042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119198/range2119198/1

https://synbiohub.org/public/igem/BBa_M36042/annotation2119198/range2119198/1
type: Range
persistent identity: range2119198
display id: range2119198
version: 1
direction: direction/forward
top level: BBa_M36042
start: 106
end: 106
orientation: inline

BBa_K613013
type: ComponentDefinition
persistent identity: BBa K613013
display id: BBa_K613013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR V36F mutant
creator: Lilia Salimova
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _785_
m user id: 0
owner id: 9696
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a TetR mutant, who carries the V36F mutation.
mutable notes: The mutation was introduced by site-directed mutagenesis
mutable provenance: The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36F mutation by site-directed mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: V to F
sequence: https://synbiohub.org/public/igem/BBa_K613013_sequence/1

V to F
type: SequenceAnnotation
persistent identity: annotation2136974
display id: annotation2136974
version: 1
top level: BBa_K613013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K613013/annotation2136974/range2136974/1

https://synbiohub.org/public/igem/BBa_K613013/annotation2136974/range2136974/1
type: Range
persistent identity: range2136974
display id: range2136974
version: 1
direction: direction/forward
top level: BBa_K613013
start: 106
end: 108
orientation: inline

BBa_K590016
type: ComponentDefinition
persistent identity: BBa K590016
display id: BBa_K590016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP_mamI_pGA1C3
creator: Michael Brasino, Alicia Wong, Rashmi Ravichandran
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Fluoroescent(sfGFP) membrane localization (mamI)
mutable notes: sequence confirmed
mutable provenance: Made from Gibson reaction (Miniprepped)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K590081
component: BBa_R0011
component: BBa_I746916
component: BBa_B0034
component: BBa_K590082
sequence annotation: BBa_K590081
sequence annotation: BBa_I746916
sequence annotation: BBa_R0011
sequence annotation: BBa_K590082
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K590016_sequence/1

BBa_K590081
type: Component
persistent identity: component2146000
display id: component2146000
version: 1
top level: BBa_K590016
access: public
definition: https://synbiohub.org/public/igem/BBa_K590081/1

BBa_R0011
type: Component
persistent identity: component2145987
display id: component2145987
version: 1
top level: BBa_K590016
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_I746916
type: Component
persistent identity: component2145997
display id: component2145997
version: 1
top level: BBa_K590016
access: public
definition: BBa_I746916

BBa_B0034
type: Component
persistent identity: component2145993
display id: component2145993
version: 1
top level: BBa_K590016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K590082
type: Component
persistent identity: component2145999
display id: component2145999
version: 1
top level: BBa_K590016
access: public
definition: https://synbiohub.org/public/igem/BBa_K590082/1

BBa_K590081
type: SequenceAnnotation
persistent identity: annotation2146000
display id: annotation2146000
version: 1
top level: BBa_K590016
location: https://synbiohub.org/public/igem/BBa_K590016/annotation2146000/range2146000/1
component: BBa_K590081

https://synbiohub.org/public/igem/BBa_K590016/annotation2146000/range2146000/1
type: Range
persistent identity: range2146000
display id: range2146000
version: 1
direction: direction/reverse
top level: BBa_K590016
start: 812
end: 1021
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2145997
display id: annotation2145997
version: 1
top level: BBa_K590016
location: https://synbiohub.org/public/igem/BBa_K590016/annotation2145997/range2145997/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K590016/annotation2145997/range2145997/1
type: Range
persistent identity: range2145997
display id: range2145997
version: 1
direction: direction/reverse
top level: BBa_K590016
start: 68
end: 787
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2145987
display id: annotation2145987
version: 1
top level: BBa_K590016
location: https://synbiohub.org/public/igem/BBa_K590016/annotation2145987/range2145987/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K590016/annotation2145987/range2145987/1
type: Range
persistent identity: range2145987
display id: range2145987
version: 1
direction: direction/forward
top level: BBa_K590016
start: 1
end: 54
orientation: inline

BBa_K590082
type: SequenceAnnotation
persistent identity: annotation2145999
display id: annotation2145999
version: 1
top level: BBa_K590016
location: https://synbiohub.org/public/igem/BBa_K590016/annotation2145999/range2145999/1
component: BBa_K590082

https://synbiohub.org/public/igem/BBa_K590016/annotation2145999/range2145999/1
type: Range
persistent identity: range2145999
display id: range2145999
version: 1
direction: direction/reverse
top level: BBa_K590016
start: 788
end: 811
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2145993
display id: annotation2145993
version: 1
top level: BBa_K590016
location: https://synbiohub.org/public/igem/BBa_K590016/annotation2145993/range2145993/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K590016/annotation2145993/range2145993/1
type: Range
persistent identity: range2145993
display id: range2145993
version: 1
direction: direction/reverse
top level: BBa_K590016
start: 56
end: 67
orientation: inline

bbc1
type: ComponentDefinition
persistent identity: BBa K398108
display id: BBa_K398108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salt tolerance cluster
creator: Ramon van der Valk
created: 2010-10-16T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A protein from Chlamydomonas sp. W80 that increases the salt tolerance of E.coli. This protein is called bbc1. The exact mechanism of the increased salt tolerance is as of yet unknown. But since bbc1 shows a high homology to RBS binding proteins, it is theorized that it may prevent/stabilize the folding structure of the ribosome under high salt stress conditions.
mutable notes: Nucleotide sequence optimized for expression in E.coli
mutable provenance: Genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J13002
component: BBa_K398100
component: BBa_B0015
sequence annotation: BBa_J13002
sequence annotation: BBa_K398100
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K398108_sequence/1

BBa_J13002
type: Component
persistent identity: component2093215
display id: component2093215
version: 1
top level: bbc1
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_K398100
type: Component
persistent identity: component2093219
display id: component2093219
version: 1
top level: bbc1
access: public
definition: https://synbiohub.org/public/igem/BBa_K398100/1

BBa_B0015
type: Component
persistent identity: component2093226
display id: component2093226
version: 1
top level: bbc1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2093215
display id: annotation2093215
version: 1
top level: bbc1
location: https://synbiohub.org/public/igem/BBa_K398108/annotation2093215/range2093215/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_K398108/annotation2093215/range2093215/1
type: Range
persistent identity: range2093215
display id: range2093215
version: 1
direction: direction/reverse
top level: bbc1
start: 1
end: 74
orientation: inline

BBa_K398100
type: SequenceAnnotation
persistent identity: annotation2093219
display id: annotation2093219
version: 1
top level: bbc1
location: https://synbiohub.org/public/igem/BBa_K398108/annotation2093219/range2093219/1
component: BBa_K398100

https://synbiohub.org/public/igem/BBa_K398108/annotation2093219/range2093219/1
type: Range
persistent identity: range2093219
display id: range2093219
version: 1
direction: direction/reverse
top level: bbc1
start: 81
end: 707
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2093226
display id: annotation2093226
version: 1
top level: bbc1
location: https://synbiohub.org/public/igem/BBa_K398108/annotation2093226/range2093226/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K398108/annotation2093226/range2093226/1
type: Range
persistent identity: range2093226
display id: range2093226
version: 1
direction: direction/reverse
top level: bbc1
start: 716
end: 844
orientation: inline

"M"
type: ComponentDefinition
persistent identity: BBa K1080004
display id: BBa_K1080004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ChlM
creator: Macquarie University
created: 2013-09-21T11:00:00Z
modified: 2016-10-20T07:01:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 7708
owner id: 18612
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sdgegter
mutable notes: sdrgdtr
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ChlM
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1080004_sequence/1

ChlM
type: SequenceAnnotation
persistent identity: annotation2525676
display id: annotation2525676
version: 1
top level: "M"
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1080004/annotation2525676/range2525676/1

https://synbiohub.org/public/igem/BBa_K1080004/annotation2525676/range2525676/1
type: Range
persistent identity: range2525676
display id: range2525676
version: 1
direction: direction/forward
top level: "M"
start: 25
end: 867
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2525675
display id: annotation2525675
version: 1
top level: "M"
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1080004/annotation2525675/range2525675/1

https://synbiohub.org/public/igem/BBa_K1080004/annotation2525675/range2525675/1
type: Range
persistent identity: range2525675
display id: range2525675
version: 1
direction: direction/forward
top level: "M"
start: 6
end: 16
orientation: inline

BBa_P10500
type: ComponentDefinition
persistent identity: BBa P10500
display id: BBa_P10500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhytoBricks Universal Acceptor
creator: Traci Haddock-Angelli
created: 2016-05-24T11:00:00Z
modified: 2016-05-25T06:06:05Z
discontinued: false
dominant: false
group u list: _2659_
m user id: 8248
owner id: 8248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the universal acceptor plasmid for creating new PhytoBricks parts. This sequence is contained within the BioBricks vector pSB1C3.
mutable notes: More information to come
mutable provenance: More information to come
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_P10500_sequence/1

BBa_K1051203
type: ComponentDefinition
persistent identity: BBa K1051203
display id: BBa_K1051203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cln2-PEST
creator: wanling ZHOU
created: 2013-06-29T11:00:00Z
modified: 2015-05-08T01:08:54Z
discontinued: false
dominant: false
group u list: _1358_
m user id: 0
owner id: 18073
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PEST
mutable notes: PEST
mutable provenance: PEST
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1051203_sequence/1

BBa_K343004
type: ComponentDefinition
persistent identity: BBa K343004
display id: BBa_K343004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Flagella overekspression
creator: Louise Linnebjerg Bohn Christoffersen, Pernille Marie Madsen, Sheila Maibom-Thomsen
created: 2010-07-04T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _465_
m user id: 0
owner id: 6087
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part entails a sigma 70 promoter, a RBS, the FlhDC master operon and a dual-terminator. This part will cause hyperflagellated cells.
mutable notes: It has not been tested yet
mutable provenance: The promoter, the terminater and the RBS are all in the spring 2010 kit. The FlhDC mater operon is fron E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_J13002
component: BBa_K343000
sequence annotation: BBa_B0015
sequence annotation: BBa_J13002
sequence annotation: BBa_K343000
sequence: https://synbiohub.org/public/igem/BBa_K343004_sequence/1

BBa_B0015
type: Component
persistent identity: component2092214
display id: component2092214
version: 1
top level: BBa_K343004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J13002
type: Component
persistent identity: component2092199
display id: component2092199
version: 1
top level: BBa_K343004
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_K343000
type: Component
persistent identity: component2092207
display id: component2092207
version: 1
top level: BBa_K343004
access: public
definition: BBa_K343000

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2092214
display id: annotation2092214
version: 1
top level: BBa_K343004
location: https://synbiohub.org/public/igem/BBa_K343004/annotation2092214/range2092214/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K343004/annotation2092214/range2092214/1
type: Range
persistent identity: range2092214
display id: range2092214
version: 1
direction: direction/reverse
top level: BBa_K343004
start: 1021
end: 1149
orientation: inline

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2092199
display id: annotation2092199
version: 1
top level: BBa_K343004
location: https://synbiohub.org/public/igem/BBa_K343004/annotation2092199/range2092199/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_K343004/annotation2092199/range2092199/1
type: Range
persistent identity: range2092199
display id: range2092199
version: 1
direction: direction/reverse
top level: BBa_K343004
start: 1
end: 74
orientation: inline

BBa_K343000
type: SequenceAnnotation
persistent identity: annotation2092207
display id: annotation2092207
version: 1
top level: BBa_K343004
location: https://synbiohub.org/public/igem/BBa_K343004/annotation2092207/range2092207/1
component: BBa_K343000

https://synbiohub.org/public/igem/BBa_K343004/annotation2092207/range2092207/1
type: Range
persistent identity: range2092207
display id: range2092207
version: 1
direction: direction/reverse
top level: BBa_K343004
start: 81
end: 1012
orientation: inline

BBa_K208000
type: ComponentDefinition
persistent identity: BBa K208000
display id: BBa_K208000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP
creator: Elisabeth Linton
created: 2009-10-07T11:00:00Z
modified: 2015-05-08T01:11:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 2425
owning group id: 303
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: GFP
mutable notes: Mutate PstI sites
mutable provenance: plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: GFP Coding Region
sequence: https://synbiohub.org/public/igem/BBa_K208000_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2062216
display id: annotation2062216
version: 1
top level: BBa_K208000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K208000/annotation2062216/range2062216/1

https://synbiohub.org/public/igem/BBa_K208000/annotation2062216/range2062216/1
type: Range
persistent identity: range2062216
display id: range2062216
version: 1
direction: direction/forward
top level: BBa_K208000
start: 1
end: 3
orientation: inline

GFP Coding Region
type: SequenceAnnotation
persistent identity: annotation2062217
display id: annotation2062217
version: 1
top level: BBa_K208000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K208000/annotation2062217/range2062217/1

https://synbiohub.org/public/igem/BBa_K208000/annotation2062217/range2062217/1
type: Range
persistent identity: range2062217
display id: range2062217
version: 1
direction: direction/forward
top level: BBa_K208000
start: 1
end: 720
orientation: inline

BBa_K912001
type: ComponentDefinition
persistent identity: BBa K912001
display id: BBa_K912001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli phytase (appa phytase)
creator: Grant Nicholas
created: 2012-07-08T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1177_
m user id: 0
owner id: 12923
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part to produce the appa e. coli phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.
mutable notes: In progress.
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/341941295?report=fasta Genomic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K912001_sequence/1

BBa_K157004
type: ComponentDefinition
persistent identity: BBa K157004
display id: BBa_K157004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluoresceine-A-binding
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from pieris brassicae), originally designed by Arne Skerra [see Ref. 1,2].
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Lipocalin FluA
sequence: https://synbiohub.org/public/igem/BBa_K157004_sequence/1

Lipocalin FluA
type: SequenceAnnotation
persistent identity: annotation2040659
display id: annotation2040659
version: 1
top level: BBa_K157004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157004/annotation2040659/range2040659/1

https://synbiohub.org/public/igem/BBa_K157004/annotation2040659/range2040659/1
type: Range
persistent identity: range2040659
display id: range2040659
version: 1
direction: direction/forward
top level: BBa_K157004
start: 1
end: 522
orientation: inline

BBa_K1031444
type: ComponentDefinition
persistent identity: BBa K1031444
display id: BBa_K1031444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J23114
sequence annotation: BBa_B0034
sequence annotation: HcaR
sequence: https://synbiohub.org/public/igem/BBa_K1031444_sequence/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2357463
display id: annotation2357463
version: 1
top level: BBa_K1031444
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031444/annotation2357463/range2357463/1

https://synbiohub.org/public/igem/BBa_K1031444/annotation2357463/range2357463/1
type: Range
persistent identity: range2357463
display id: range2357463
version: 1
direction: direction/forward
top level: BBa_K1031444
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357465
display id: annotation2357465
version: 1
top level: BBa_K1031444
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031444/annotation2357465/range2357465/1

https://synbiohub.org/public/igem/BBa_K1031444/annotation2357465/range2357465/1
type: Range
persistent identity: range2357465
display id: range2357465
version: 1
direction: direction/forward
top level: BBa_K1031444
start: 44
end: 55
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357467
display id: annotation2357467
version: 1
top level: BBa_K1031444
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031444/annotation2357467/range2357467/1

https://synbiohub.org/public/igem/BBa_K1031444/annotation2357467/range2357467/1
type: Range
persistent identity: range2357467
display id: range2357467
version: 1
direction: direction/forward
top level: BBa_K1031444
start: 62
end: 952
orientation: inline

BBa_K608017
type: ComponentDefinition
persistent identity: BBa K608017
display id: BBa_K608017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium promoter and medium RBS with RFP
creator: Julia M??ller
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promotor,RBS with RFP
mutable notes: composite
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E1010
component: BBa_K608006
sequence annotation: BBa_K608006
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K608017_sequence/1

BBa_E1010
type: Component
persistent identity: component2247929
display id: component2247929
version: 1
top level: BBa_K608017
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K608006
type: Component
persistent identity: component2247926
display id: component2247926
version: 1
top level: BBa_K608017
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2247926
display id: annotation2247926
version: 1
top level: BBa_K608017
location: https://synbiohub.org/public/igem/BBa_K608017/annotation2247926/range2247926/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K608017/annotation2247926/range2247926/1
type: Range
persistent identity: range2247926
display id: range2247926
version: 1
direction: direction/reverse
top level: BBa_K608017
start: 1
end: 56
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247929
display id: annotation2247929
version: 1
top level: BBa_K608017
location: https://synbiohub.org/public/igem/BBa_K608017/annotation2247929/range2247929/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K608017/annotation2247929/range2247929/1
type: Range
persistent identity: range2247929
display id: range2247929
version: 1
direction: direction/reverse
top level: BBa_K608017
start: 63
end: 768
orientation: inline

BBa_K199021
type: ComponentDefinition
persistent identity: BBa K199021
display id: BBa_K199021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-RFP (all inverted)
creator: Kin Lau
created: 2009-07-07T11:00:00Z
modified: 2015-05-08T01:11:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5395
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is oriented opposite to typical BioBrick arrangement in terms of placement of BioBrick prefix and suffix. This part can be ligated to a promoter to measure promoter activity.
mutable notes: none
mutable provenance: Ligation of existing parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J44001
component: BBa_J31008
sequence annotation: BBa_J44001
sequence annotation: BBa_J31008
sequence: https://synbiohub.org/public/igem/BBa_K199021_sequence/1

BBa_J44001
type: Component
persistent identity: component2010329
display id: component2010329
version: 1
top level: BBa_K199021
access: public
definition: https://synbiohub.org/public/igem/BBa_J44001/1

BBa_J31008
type: Component
persistent identity: component2010327
display id: component2010327
version: 1
top level: BBa_K199021
access: public
definition: mRFPr

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2010329
display id: annotation2010329
version: 1
top level: BBa_K199021
location: https://synbiohub.org/public/igem/BBa_K199021/annotation2010329/range2010329/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_K199021/annotation2010329/range2010329/1
type: Range
persistent identity: range2010329
display id: range2010329
version: 1
direction: direction/reverse
top level: BBa_K199021
start: 687
end: 701
orientation: inline

BBa_J31008
type: SequenceAnnotation
persistent identity: annotation2010327
display id: annotation2010327
version: 1
top level: BBa_K199021
location: https://synbiohub.org/public/igem/BBa_K199021/annotation2010327/range2010327/1
component: BBa_J31008

https://synbiohub.org/public/igem/BBa_K199021/annotation2010327/range2010327/1
type: Range
persistent identity: range2010327
display id: range2010327
version: 1
direction: direction/reverse
top level: BBa_K199021
start: 1
end: 678
orientation: inline