iGEM 2019 Distribution Plate 6 Well 13D
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iGEM 2019 Distribution Plate 6 Well 13C
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iGEM 2019 Distribution Plate 6 Well 21P
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iGEM 2019 Distribution Plate 6 Well 23O
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iGEM 2019 Distribution Plate 6 Well 8D
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iGEM 2019 Distribution Plate 6 Well 10P
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iGEM 2019 Distribution Plate 6 Well 21G
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iGEM 2019 Distribution Plate 6 Well 13F
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iGEM 2019 Distribution Plate 6 Well 17G
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iGEM 2019 Distribution Plate 6 Well 17A
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iGEM 2019 Distribution Plate 6 Well 10N
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iGEM 2019 Distribution Plate 6 Well 8B
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iGEM 2019 Distribution Plate 6 Well 15D
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iGEM 2019 Distribution Plate 6 Well 12A
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iGEM 2019 Distribution Plate 6 Well 1A
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iGEM 2019 Distribution Plate 6 Well 8A
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iGEM 2019 Distribution Plate 6 Well 10J
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iGEM 2019 Distribution Plate 6 Well 7E
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iGEM 2019 Distribution Plate 6 Well 1O
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iGEM 2019 Distribution Plate 6 Well 3C
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iGEM 2019 Distribution Plate 6 Well 5A
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iGEM 2019 Distribution Plate 6 Well 5G
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iGEM 2019 Distribution Plate 6 Well 3F
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iGEM 2019 Distribution Plate 6 Well 21F
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iGEM 2019 Distribution Plate 6 Well 19G
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iGEM 2019 Distribution Plate 6 Well 2I
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iGEM 2019 Distribution Plate 6 Well 17I
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iGEM 2019 Distribution Plate 6 Well 9D
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iGEM 2019 Distribution Plate 6 Well 10L
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iGEM 2019 Distribution Plate 6 Well 3N
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iGEM 2019 Distribution Plate 6 Well 15L
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iGEM 2019 Distribution Plate 6 Well 19F
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iGEM 2019 Distribution Plate 6 Well 4J
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iGEM 2019 Distribution Plate 6 Well 17L
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iGEM 2019 Distribution Plate 6 Well 12C
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iGEM 2019 Distribution Plate 6 Well 15F
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iGEM 2019 Distribution Plate 6 Well 15A
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iGEM 2019 Distribution Plate 6 Well 11J
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iGEM 2019 Distribution Plate 6 Well 2P
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iGEM 2019 Distribution Plate 6 Well 3P
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iGEM 2019 Distribution Plate 6 Well 1G
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iGEM 2019 Distribution Plate 6 Well 17E
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iGEM 2019 Distribution Plate 6 Well 1E
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iGEM 2019 Distribution Plate 6 Well 8C
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iGEM 2019 Distribution Plate 6 Well 19K
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iGEM 2019 Distribution Plate 6 Well 10F
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iGEM 2019 Distribution Plate 6 Well 5B
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iGEM 2019 Distribution Plate 6 Well 23G
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iGEM 2019 Distribution Plate 6 Well 7D
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iGEM 2019 Distribution Plate 6 Well 6B
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iGEM 2019 Distribution Plate 6 Well 1H
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iGEM 2019 Distribution Plate 6 Well 4B
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iGEM 2019 Distribution Plate 6 Well 1I
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iGEM 2019 Distribution Plate 6 Well 23M
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iGEM 2019 Distribution Plate 6 Well 17N
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iGEM 2019 Distribution Plate 6 Well 23I
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iGEM 2019 Distribution Plate 6 Well 3H
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iGEM 2019 Distribution Plate 6 Well 21B
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iGEM 2019 Distribution Plate 6 Well 15H
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iGEM 2019 Distribution Plate 6 Well 6P
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iGEM 2019 Distribution Plate 6 Well 18F
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iGEM 2019 Distribution Plate 6 Well 2B
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iGEM 2019 Distribution Plate 6 Well 1C
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iGEM 2019 Distribution Plate 6 Well 11E
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iGEM 2019 Distribution Plate 6 Well 23J
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iGEM 2019 Distribution Plate 6 Well 19H
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iGEM 2019 Distribution Plate 6 Well 2N
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iGEM 2019 Distribution Plate 6 Well 9K
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iGEM 2019 Distribution Plate 6 Well 19I
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iGEM 2019 Distribution Plate 6 Well 6D
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iGEM 2019 Distribution Plate 6 Well 14A
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iGEM 2019 Distribution Plate 6 Well 12B
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iGEM 2019 Distribution Plate 6 Well 5I
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iGEM 2019 Distribution Plate 6 Well 21D
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iGEM 2019 Distribution Plate 6 Well 3E
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iGEM 2019 Distribution Plate 6 Well 15B
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iGEM 2019 Distribution Plate 6 Well 2K
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iGEM 2019 Distribution Plate 6 Well 15E
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iGEM 2019 Distribution Plate 6 Well 23A
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iGEM 2019 Distribution Plate 6 Well 4L
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iGEM 2019 Distribution Plate 6 Well 5P
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iGEM 2019 Distribution Plate 6 Well 4H
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iGEM 2019 Distribution Plate 6 Well 15C
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iGEM 2019 Distribution Plate 6 Well 17H
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iGEM 2019 Distribution Plate 6 Well 19N
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iGEM 2019 Distribution Plate 6 Well 5L
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iGEM 2019 Distribution Plate 6 Well 5M
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iGEM 2019 Distribution Plate 6 Well 7F
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iGEM 2019 Distribution Plate 6 Well 10B
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version: 1
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member: BBa_K1467102

iGEM 2019 Distribution Plate 6 Well 22D
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version: 1
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member: https://synbiohub.org/public/igem/BBa_J04454/1

iGEM 2019 Distribution Plate 6 Well 6H
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iGEM 2019 Distribution Plate 6 Well 23L
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member: https://synbiohub.org/public/igem/BBa_J364009/1

iGEM 2019 Distribution Plate 6 Well 21J
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member: BBa_K1333005

iGEM 2019 Distribution Plate 6 Well 12J
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iGEM 2019 Distribution Plate 6 Well 13B
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member: BBa_K1073024

iGEM 2019 Distribution Plate 6 Well 23E
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iGEM 2019 Distribution Plate 6 Well 3I
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member: c2 P22

iGEM 2019 Distribution Plate 6 Well 21C
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member: BBa_I13452

iGEM 2019 Distribution Plate 6 Well 19P
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member: BBa_K1614000

iGEM 2019 Distribution Plate 6 Well 8N
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iGEM 2019 Distribution Plate 6 Well 19A
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iGEM 2019 Distribution Plate 6 Well 8H
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iGEM 2019 Distribution Plate 6 Well 21I
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iGEM 2019 Distribution Plate 6 Well 8J
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iGEM 2019 Distribution Plate 6 Well 3G
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iGEM 2019 Distribution Plate 6 Well 13E
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member: BBa_S03154

iGEM 2019 Distribution Plate 6 Well 6F
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member: BBa_K1114102

iGEM 2019 Distribution Plate 6 Well 17C
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member: BBa_J23100

iGEM 2019 Distribution Plate 6 Well 10D
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member: BBa_K1467103

iGEM 2019 Distribution Plate 6 Well 7H
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member: BBa_K1033915

iGEM 2019 Distribution Plate 6 Well 3D
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member: BBa_K797002

iGEM 2019 Distribution Plate 6 Well 10A
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version: 1
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member: BBa_J04450

iGEM 2019 Distribution Plate 6 Well 5E
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iGEM 2019 Distribution Plate 6 Well 6C
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iGEM 2019 Distribution Plate 6 Well 7B
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iGEM 2019 Distribution Plate 6 Well 20F
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iGEM 2019 Distribution Plate 6 Well 9L
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iGEM 2019 Distribution Plate 6 Well 2G
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iGEM 2019 Distribution Plate 6 Well 9M
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iGEM 2019 Distribution Plate 6 Well 13L
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iGEM 2019 Distribution Plate 6 Well 7K
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iGEM 2019 Distribution Plate 6 Well 7I
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iGEM 2019 Distribution Plate 6 Well 2L
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iGEM 2019 Distribution Plate 6 Well 14B
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iGEM 2019 Distribution Plate 6 Well 3L
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iGEM 2019 Distribution Plate 6 Well 7P
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iGEM 2019 Distribution Plate 6 Well 19M
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iGEM 2019 Distribution Plate 6 Well 20D
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iGEM 2019 Distribution Plate 6 Well 7M
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iGEM 2019 Distribution Plate 6 Well 20B
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iGEM 2019 Distribution Plate 6 Well 4E
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iGEM 2019 Distribution Plate 6 Well 2A
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iGEM 2019 Distribution Plate 6 Well 4N
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iGEM 2019 Distribution Plate 6 Well 11M
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iGEM 2019 Distribution Plate 6 Well 21M
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iGEM 2019 Distribution Plate 6 Well 13I
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iGEM 2019 Distribution Plate 6 Well 18P
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iGEM 2019 Distribution Plate 6 Well 9N
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iGEM 2019 Distribution Plate 6 Well 5O
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iGEM 2019 Distribution Plate 6 Well 17D
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iGEM 2019 Distribution Plate 6 Well 12H
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iGEM 2019 Distribution Plate 6 Well 17B
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iGEM 2019 Distribution Plate 6 Well 18J
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iGEM 2019 Distribution Plate 6 Well 6J
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iGEM 2019 Distribution Plate 6 Well 3A
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iGEM 2019 Distribution Plate 6 Well 15O
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iGEM 2019 Distribution Plate 6 Well 21K
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iGEM 2019 Distribution Plate 6 Well 11H
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iGEM 2019 Distribution Plate 6 Well 11P
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iGEM 2019 Distribution Plate 6 Well 19O
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iGEM 2019 Distribution Plate 6 Well 5H
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iGEM 2019 Distribution Plate 6 Well 23F
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iGEM 2019 Distribution Plate 6 Well 13M
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iGEM 2019 Distribution Plate 6 Well 15N
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iGEM 2019 Distribution Plate 6 Well 4C
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iGEM 2019 Distribution Plate 6 Well 5J
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iGEM 2019 Distribution Plate 6 Well 11L
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iGEM 2019 Distribution Plate 6 Well 4F
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iGEM 2019 Distribution Plate 6 Well 11C
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iGEM 2019 Distribution Plate 6 Well 22H
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iGEM 2019 Distribution Plate 6 Well 15P
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iGEM 2019 Distribution Plate 6 Well 1F
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iGEM 2019 Distribution Plate 6 Well 2H
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iGEM 2019 Distribution Plate 6 Well 5F
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iGEM 2019 Distribution Plate 6 Well 9G
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iGEM 2019 Distribution Plate 6 Well 5K
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iGEM 2019 Distribution Plate 6 Well 21A
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iGEM 2019 Distribution Plate 6 Well 13N
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iGEM 2019 Distribution Plate 6 Well 7J
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iGEM 2019 Distribution Plate 6 Well 12L
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iGEM 2019 Distribution Plate 6 Well 23D
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iGEM 2019 Distribution Plate 6 Well 9H
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iGEM 2019 Distribution Plate 6 Well 17F
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iGEM 2019 Distribution Plate 6 Well 1N
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iGEM 2019 Distribution Plate 6 Well 14H
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member: https://synbiohub.org/public/igem/BBa_J04455/1

iGEM 2019 Distribution Plate 6 Well 3O
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iGEM 2019 Distribution Plate 6 Well 12P
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iGEM 2019 Distribution Plate 6 Well 4P
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iGEM 2019 Distribution Plate 6 Well 6G
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iGEM 2019 Distribution Plate 6 Well 13A
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iGEM 2019 Distribution Plate 6 Well 7C
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iGEM 2019 Distribution Plate 6 Well 22F
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iGEM 2019 Distribution Plate 6 Well 9F
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iGEM 2019 Distribution Plate 6 Well 9E
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iGEM 2019 Distribution Plate 6 Well 13J
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iGEM 2019 Distribution Plate 6 Well 1L
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iGEM 2019 Distribution Plate 6 Well 8F
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iGEM 2019 Distribution Plate 6 Well 12F
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iGEM 2019 Distribution Plate 6 Well 10H
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iGEM 2019 Distribution Plate 6 Well 17P
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iGEM 2019 Distribution Plate 6 Well 11N
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iGEM 2019 Distribution Plate 6 Well 11F
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iGEM 2019 Distribution Plate 6 Well 15I
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iGEM 2019 Distribution Plate 6 Well 20H
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iGEM 2019 Distribution Plate 6 Well 13K
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iGEM 2019 Distribution Plate 6 Well 11D
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member: BBa_K1159102

iGEM 2019 Distribution Plate 6 Well 18D
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iGEM 2019 Distribution Plate 6 Well 7N
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iGEM 2019 Distribution Plate 6 Well 2C
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member: BBa_J04450

iGEM 2019 Distribution Plate 6 Well 19C
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iGEM 2019 Distribution Plate 6 Well 4A
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member: BBa_J04450

iGEM 2019 Distribution Plate 6 Well 11B
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member: BBa_K1159101

iGEM 2019 Distribution Plate 6 Well 1D
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member: BBa_K352003

iGEM 2019 Distribution Plate 6 Well 9I
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iGEM 2019 Distribution Plate 6 Well 5N
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iGEM 2019 Distribution Plate 6 Well 17J
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iGEM 2019 Distribution Plate 6 Well 2J
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iGEM 2019 Distribution Plate 6 Well 9J
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member: BBa_K1033929

iGEM 2019 Distribution Plate 6 Well 6E
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member: BBa_J04450

iGEM 2019 Distribution Plate 6 Well 14F
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member: https://synbiohub.org/public/igem/BBa_J04455/1

iGEM 2019 Distribution Plate 6 Well 8L
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iGEM 2019 Distribution Plate 6 Well 1K
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iGEM 2019 Distribution Plate 6 Well 13P
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iGEM 2019 Distribution Plate 6 Well 6A
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iGEM 2019 Distribution Plate 6 Well 23K
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iGEM 2019 Distribution Plate 6 Well 3B
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iGEM 2019 Distribution Plate 6 Well 5C
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iGEM 2019 Distribution Plate 6 Well 21E
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member: BBa_I13500

iGEM 2019 Distribution Plate 6 Well 11A
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iGEM 2019 Distribution Plate 6 Well 9B
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iGEM 2019 Distribution Plate 6 Well 13O
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iGEM 2019 Distribution Plate 6 Well 15J
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member: BBa_K1390004

iGEM 2019 Distribution Plate 6 Well 1B
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member: BBa_K352002

iGEM 2019 Distribution Plate 6 Well 22B
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version: 1
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member: https://synbiohub.org/public/igem/BBa_J04454/1

iGEM 2019 Distribution Plate 6 Well 1M
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persistent identity: iGEM 2019 Plate6 Well1M
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iGEM 2019 Distribution Plate 6 Well 19B
type: Collection
persistent identity: iGEM 2019 Plate6 Well19B
display id: iGEM_2019_Plate6_Well19B
version: 1
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iGEM 2019 Distribution Plate 6
type: Collection
persistent identity: iGEM 2019 Plate6
display id: iGEM_2019_Plate6
version: 1
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member: iGEM 2019 Distribution Plate 6 Well 7M
member: BBa_F2622
member: BBa_J23114
member: BBa_K1033915
member: BBa_K1159101
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member: iGEM 2019 Distribution Plate 6 Well 1M
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member: iGEM 2019 Distribution Plate 6 Well 5I
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member: BBa_S03154
member: iGEM 2019 Distribution Plate 6 Well 9D
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member: BBa_K1159102
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member: iGEM 2019 Distribution Plate 6 Well 11E
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member: iGEM 2019 Distribution Plate 6 Well 17E
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member: BBa_K731500
member: iGEM 2019 Distribution Plate 6 Well 21M
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member: iGEM 2019 Distribution Plate 6 Well 17C
member: cinR
member: iGEM 2019 Distribution Plate 6 Well 19I
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member: https://synbiohub.org/public/igem/BBa_J04452/1
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member: iGEM 2019 Distribution Plate 6 Well 15M
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member: BBa_K1114010
member: iGEM 2019 Distribution Plate 6 Well 23C
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member: iGEM 2019 Distribution Plate 6 Well 19G
member: rib-synth
member: iGEM 2019 Distribution Plate 6 Well 21A
member: iGEM 2019 Distribution Plate 6 Well 13G
member: iGEM 2019 Distribution Plate 6 Well 15O
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member: iGEM 2019 Distribution Plate 6 Well 19J
member: iGEM 2019 Distribution Plate 6 Well 15B
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member: iGEM 2019 Distribution Plate 6 Well 12A

iGEM 2019 Distribution Plate 6 Well 9P
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persistent identity: iGEM 2019 Plate6 Well9P
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iGEM 2019 Distribution Plate 6 Well 15G
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persistent identity: iGEM 2019 Plate6 Well15G
display id: iGEM_2019_Plate6_Well15G
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iGEM 2019 Distribution Plate 6 Well 1J
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persistent identity: iGEM 2019 Plate6 Well1J
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iGEM 2019 Distribution Plate 6 Well 19J
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persistent identity: iGEM 2019 Plate6 Well19J
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iGEM 2019 Distribution Plate 6 Well 9C
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iGEM 2019 Distribution Plate 6 Well 18H
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iGEM 2019 Distribution Plate 6 Well 15K
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iGEM 2019 Distribution Plate 6 Well 18N
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persistent identity: iGEM 2019 Plate6 Well18N
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iGEM 2019 Distribution Plate 6 Well 2E
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persistent identity: iGEM 2019 Plate6 Well2E
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iGEM 2019 Distribution Plate 6 Well 7O
type: Collection
persistent identity: iGEM 2019 Plate6 Well7O
display id: iGEM_2019_Plate6_Well7O
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iGEM 2019 Distribution Plate 6 Well 23H
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persistent identity: iGEM 2019 Plate6 Well23H
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iGEM 2019 Distribution Plate 6 Well 17O
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persistent identity: iGEM 2019 Plate6 Well17O
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iGEM 2019 Distribution Plate 6 Well 21L
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persistent identity: iGEM 2019 Plate6 Well21L
display id: iGEM_2019_Plate6_Well21L
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iGEM 2019 Distribution Plate 6 Well 2F
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persistent identity: iGEM 2019 Plate6 Well2F
display id: iGEM_2019_Plate6_Well2F
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member: BBa_K1114003

iGEM 2019 Distribution Plate 6 Well 19L
type: Collection
persistent identity: iGEM 2019 Plate6 Well19L
display id: iGEM_2019_Plate6_Well19L
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iGEM 2019 Distribution Plate 6 Well 6N
type: Collection
persistent identity: iGEM 2019 Plate6 Well6N
display id: iGEM_2019_Plate6_Well6N
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member: BBa_K1114108

iGEM 2019 Distribution Plate 6 Well 4D
type: Collection
persistent identity: iGEM 2019 Plate6 Well4D
display id: iGEM_2019_Plate6_Well4D
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member: BBa_K1114014

iGEM 2019 Distribution Plate 6 Well 14D
type: Collection
persistent identity: iGEM 2019 Plate6 Well14D
display id: iGEM_2019_Plate6_Well14D
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owned by: myers
member: https://synbiohub.org/public/igem/BBa_J04455/1

iGEM 2019 Distribution Plate 6 Well 21H
type: Collection
persistent identity: iGEM 2019 Plate6 Well21H
display id: iGEM_2019_Plate6_Well21H
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iGEM 2019 Distribution Plate 6 Well 9O
type: Collection
persistent identity: iGEM 2019 Plate6 Well9O
display id: iGEM_2019_Plate6_Well9O
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iGEM 2019 Distribution Plate 6 Well 23B
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persistent identity: iGEM 2019 Plate6 Well23B
display id: iGEM_2019_Plate6_Well23B
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member: https://synbiohub.org/public/igem/BBa_J364000/1

iGEM 2019 Distribution Plate 6 Well 18L
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persistent identity: iGEM 2019 Plate6 Well18L
display id: iGEM_2019_Plate6_Well18L
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member: BBa_I746101

iGEM 2019 Distribution Plate 6 Well 23C
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persistent identity: iGEM 2019 Plate6 Well23C
display id: iGEM_2019_Plate6_Well23C
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member: spacer

iGEM 2019 Distribution Plate 6 Well 12D
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persistent identity: iGEM 2019 Plate6 Well12D
display id: iGEM_2019_Plate6_Well12D
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persistent identity: iGEM 2019 Plate6 Well13G
display id: iGEM_2019_Plate6_Well13G
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member: BBa_E0044

iGEM 2019 Distribution Plate 6 Well 3K
type: Collection
persistent identity: iGEM 2019 Plate6 Well3K
display id: iGEM_2019_Plate6_Well3K
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member: aiiA

iGEM 2019 Distribution Plate 6 Well 8P
type: Collection
persistent identity: iGEM 2019 Plate6 Well8P
display id: iGEM_2019_Plate6_Well8P
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member: BBa_K1467101

iGEM 2019 Distribution Plate 6 Well 19D
type: Collection
persistent identity: iGEM 2019 Plate6 Well19D
display id: iGEM_2019_Plate6_Well19D
version: 1
owned by: myers
member: Const gR4m

iGEM 2019 Distribution Plate 6 Well 13H
type: Collection
persistent identity: iGEM 2019 Plate6 Well13H
display id: iGEM_2019_Plate6_Well13H
version: 1
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member: Val-Ind

iGEM 2019 Distribution Plate 6 Well 11K
type: Collection
persistent identity: iGEM 2019 Plate6 Well11K
display id: iGEM_2019_Plate6_Well11K
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member: BBa_I13401

iGEM 2019 Distribution Plate 6 Well 21O
type: Collection
persistent identity: iGEM 2019 Plate6 Well21O
display id: iGEM_2019_Plate6_Well21O
version: 1
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member: BBa_I13507

iGEM 2019 Distribution Plate 6 Well 1P
type: Collection
persistent identity: iGEM 2019 Plate6 Well1P
display id: iGEM_2019_Plate6_Well1P
version: 1
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member: BBa_K729002

iGEM 2019 Distribution Plate 6 Well 3J
type: Collection
persistent identity: iGEM 2019 Plate6 Well3J
display id: iGEM_2019_Plate6_Well3J
version: 1
owned by: myers
member: BBa_K737016

iGEM 2019 Distribution Plate 6 Well 19E
type: Collection
persistent identity: iGEM 2019 Plate6 Well19E
display id: iGEM_2019_Plate6_Well19E
version: 1
owned by: myers
member: BBa_J23112

iGEM 2019 Distribution Plate 6 Well 11G
type: Collection
persistent identity: iGEM 2019 Plate6 Well11G
display id: iGEM_2019_Plate6_Well11G
version: 1
owned by: myers
member: BBa_F2622

iGEM 2019 Distribution Plate 6 Well 21N
type: Collection
persistent identity: iGEM 2019 Plate6 Well21N
display id: iGEM_2019_Plate6_Well21N
version: 1
owned by: myers
member: BBa_K1319004

iGEM 2019 Distribution Plate 6 Well 18B
type: Collection
persistent identity: iGEM 2019 Plate6 Well18B
display id: iGEM_2019_Plate6_Well18B
version: 1
owned by: myers
member: T7pol

iGEM 2019 Distribution Plate 6 Well 3M
type: Collection
persistent identity: iGEM 2019 Plate6 Well3M
display id: iGEM_2019_Plate6_Well3M
version: 1
owned by: myers
member: luxI

iGEM 2019 Distribution Plate 6 Well 7A
type: Collection
persistent identity: iGEM 2019 Plate6 Well7A
display id: iGEM_2019_Plate6_Well7A
version: 1
owned by: myers
member: lux pL

iGEM 2019 Distribution Plate 6 Well 11I
type: Collection
persistent identity: iGEM 2019 Plate6 Well11I
display id: iGEM_2019_Plate6_Well11I
version: 1
owned by: myers
member: BBa_I13602

iGEM 2019 Distribution Plate 6 Well 17M
type: Collection
persistent identity: iGEM 2019 Plate6 Well17M
display id: iGEM_2019_Plate6_Well17M
version: 1
owned by: myers
member: BBa_J23105

iGEM 2019 Distribution Plate 6 Well 23P
type: Collection
persistent identity: iGEM 2019 Plate6 Well23P
display id: iGEM_2019_Plate6_Well23P
version: 1
owned by: myers
member: BBa_I20270

iGEM 2019 Distribution Plate 6 Well 2D
type: Collection
persistent identity: iGEM 2019 Plate6 Well2D
display id: iGEM_2019_Plate6_Well2D
version: 1
owned by: myers
member: BBa_K1114002

iGEM 2019 Distribution Plate 6 Well 6L
type: Collection
persistent identity: iGEM 2019 Plate6 Well6L
display id: iGEM_2019_Plate6_Well6L
version: 1
owned by: myers
member: BBa_K1114107

iGEM 2019 Distribution Plate 6 Well 5D
type: Collection
persistent identity: iGEM 2019 Plate6 Well5D
display id: iGEM_2019_Plate6_Well5D
version: 1
owned by: myers
member: BBa_K1231000

iGEM 2019 Distribution Plate 6 Well 17K
type: Collection
persistent identity: iGEM 2019 Plate6 Well17K
display id: iGEM_2019_Plate6_Well17K
version: 1
owned by: myers
member: BBa_J23104

iGEM 2019 Distribution Plate 6 Well 7G
type: Collection
persistent identity: iGEM 2019 Plate6 Well7G
display id: iGEM_2019_Plate6_Well7G
version: 1
owned by: myers
member: lux pR

iGEM 2019 Distribution Plate 6 Well 7L
type: Collection
persistent identity: iGEM 2019 Plate6 Well7L
display id: iGEM_2019_Plate6_Well7L
version: 1
owned by: myers
member: BBa_K1033923

iGEM 2019 Distribution Plate 6 Well 15M
type: Collection
persistent identity: iGEM 2019 Plate6 Well15M
display id: iGEM_2019_Plate6_Well15M
version: 1
owned by: myers
member: OmpR

iGEM 2019 Distribution Plate 6 Well 9A
type: Collection
persistent identity: iGEM 2019 Plate6 Well9A
display id: iGEM_2019_Plate6_Well9A
version: 1
owned by: myers
member: BBa_S0105

iGEM 2019 Distribution Plate 6 Well 11O
type: Collection
persistent identity: iGEM 2019 Plate6 Well11O
display id: iGEM_2019_Plate6_Well11O
version: 1
owned by: myers
member: GFP genera

BBa_K1114111
type: ComponentDefinition
persistent identity: BBa K1114111
display id: BBa_K1114111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic design RBS 16, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 16 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 16 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BCD16
sequence annotation: MoClo Fusion Site B
sequence annotation: MoClo Fusion Site C
sequence: https://synbiohub.org/public/igem/BBa_K1114111_sequence/1

BCD16
type: SequenceAnnotation
persistent identity: annotation2361887
display id: annotation2361887
version: 1
top level: BBa_K1114111
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114111/annotation2361887/range2361887/1

https://synbiohub.org/public/igem/BBa_K1114111/annotation2361887/range2361887/1
type: Range
persistent identity: range2361887
display id: range2361887
version: 1
direction: direction/forward
top level: BBa_K1114111
start: 5
end: 88
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361885
display id: annotation2361885
version: 1
top level: BBa_K1114111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114111/annotation2361885/range2361885/1

https://synbiohub.org/public/igem/BBa_K1114111/annotation2361885/range2361885/1
type: Range
persistent identity: range2361885
display id: range2361885
version: 1
direction: direction/forward
top level: BBa_K1114111
start: 1
end: 4
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361886
display id: annotation2361886
version: 1
top level: BBa_K1114111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114111/annotation2361886/range2361886/1

https://synbiohub.org/public/igem/BBa_K1114111/annotation2361886/range2361886/1
type: Range
persistent identity: range2361886
display id: range2361886
version: 1
direction: direction/forward
top level: BBa_K1114111
start: 89
end: 92
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0062
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 55
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
direction: direction/forward
top level: lux pR
start: 53
end: 53
orientation: inline

BBa_K1159101
type: ComponentDefinition
persistent identity: BBa K1159101
display id: BBa_K1159101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: N-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary C-terminal half can be found under BBa_K1159102. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
mutable notes: x
mutable provenance: ''Tabacco Etch Virus''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: N-terminal half of TEV Protease
sequence: https://synbiohub.org/public/igem/BBa_K1159101_sequence/1

N-terminal half of TEV Protease
type: SequenceAnnotation
persistent identity: annotation2341473
display id: annotation2341473
version: 1
top level: BBa_K1159101
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159101/annotation2341473/range2341473/1

https://synbiohub.org/public/igem/BBa_K1159101/annotation2341473/range2341473/1
type: Range
persistent identity: range2341473
display id: range2341473
version: 1
direction: direction/forward
top level: BBa_K1159101
start: 1
end: 354
orientation: inline

BBa_K811005
type: ComponentDefinition
persistent identity: BBa K811005
display id: BBa_K811005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: INPNC-MCS
creator: Avin Veerakumar
created: 2012-10-02T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: false
group u list: _1068_
m user id: 0
owner id: 8242
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: See BBa_K811003 for details. INPNC with a MCS on C terminus with intervening GS linker domain for surface display of large proteins.
mutable notes: Optimized for expression in E. coli.
mutable provenance: Synthesized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K811005_sequence/1

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (RhlR & C4-HSL regulated)
creator: Ronny Krashinsky
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -35
sequence annotation: -10
sequence annotation: RhlR
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
direction: direction/forward
top level: RhlR+C4
start: 53
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
direction: direction/forward
top level: RhlR+C4
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
direction: direction/forward
top level: RhlR+C4
start: 42
end: 47
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
direction: direction/forward
top level: RhlR+C4
start: 1
end: 20
orientation: inline

Const gR4m
type: ComponentDefinition
persistent identity: BBa K1026002
display id: BBa_K1026002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutively Expressed gRNA targeting mRFP
creator: Hongyi WU
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:08:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1333_
m user id: 0
owner id: 12098
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This Constitutively Expressed gRNA targeting mRFP is a no-BioBrick-scars assembly of the constitutive promoter BBa_J23100, DNA sequence of a gRNA that targets mRFP reporter and a reliable terminator BBa_B0015. This gRNA sequence comes from the following literature: QI, LEI S., LARSON, MATTHEW H., GILBERT, LUKE A., DOUDNA, JENNIFER A., WEISSMAN, JONATHAN S., ARKIN, ADAM P. & LIM, WENDELL A. 2013. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell, 152, 1173-1183.
mutable notes: gRNA shall be exactly transcribed out. Any extra nucleotides would potentially jeopardize the normal function of the gRNA. Therefore, BioBrick scars are not allowed here. Instead of cut-and-ligation, inverse PCR or In-Fusion is preferred for the assembly task. In addition, a reliable terminator is applied here to ensure the termination of gRNA.
mutable provenance: The gRNA sequence is de novo synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1026003
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0015
sequence annotation: BBa_K1026003
sequence: https://synbiohub.org/public/igem/BBa_K1026002_sequence/1

BBa_B0015
type: Component
persistent identity: component2347382
display id: component2347382
version: 1
top level: Const gR4m
access: public
definition: BBa_B0015

BBa_K1026003
type: Component
persistent identity: component2347375
display id: component2347375
version: 1
top level: Const gR4m
access: public
definition: https://synbiohub.org/public/igem/BBa_K1026003/1

BBa_J23100
type: Component
persistent identity: component2347374
display id: component2347374
version: 1
top level: Const gR4m
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2347374
display id: annotation2347374
version: 1
top level: Const gR4m
location: https://synbiohub.org/public/igem/BBa_K1026002/annotation2347374/range2347374/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1026002/annotation2347374/range2347374/1
type: Range
persistent identity: range2347374
display id: range2347374
version: 1
direction: direction/reverse
top level: Const gR4m
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2347382
display id: annotation2347382
version: 1
top level: Const gR4m
location: https://synbiohub.org/public/igem/BBa_K1026002/annotation2347382/range2347382/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1026002/annotation2347382/range2347382/1
type: Range
persistent identity: range2347382
display id: range2347382
version: 1
direction: direction/reverse
top level: Const gR4m
start: 138
end: 266
orientation: inline

BBa_K1026003
type: SequenceAnnotation
persistent identity: annotation2347375
display id: annotation2347375
version: 1
top level: Const gR4m
location: https://synbiohub.org/public/igem/BBa_K1026002/annotation2347375/range2347375/1
component: BBa_K1026003

https://synbiohub.org/public/igem/BBa_K1026002/annotation2347375/range2347375/1
type: Range
persistent identity: range2347375
display id: range2347375
version: 1
direction: direction/reverse
top level: Const gR4m
start: 36
end: 137
orientation: inline

BBa_I13003
type: ComponentDefinition
persistent identity: BBa I13003
display id: BBa_I13003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium RBS, YFP (-LVA), double terminator
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0030
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13003_sequence/1

BBa_B0012
type: Component
persistent identity: component943495
display id: component943495
version: 1
top level: BBa_I13003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943485
display id: component943485
version: 1
top level: BBa_I13003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component943480
display id: component943480
version: 1
top level: BBa_I13003
access: public
definition: eyfp

BBa_B0032
type: Component
persistent identity: component943477
display id: component943477
version: 1
top level: BBa_I13003
access: public
definition: BBa_B0032

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943485
display id: annotation943485
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943485/range943485/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13003/annotation943485/range943485/1
type: Range
persistent identity: range943485
display id: range943485
version: 1
direction: direction/forward
top level: BBa_I13003
start: 751
end: 830
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation943477
display id: annotation943477
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943477/range943477/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13003/annotation943477/range943477/1
type: Range
persistent identity: range943477
display id: range943477
version: 1
direction: direction/forward
top level: BBa_I13003
start: 1
end: 13
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943495
display id: annotation943495
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943495/range943495/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13003/annotation943495/range943495/1
type: Range
persistent identity: range943495
display id: range943495
version: 1
direction: direction/forward
top level: BBa_I13003
start: 839
end: 879
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943480
display id: annotation943480
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943480/range943480/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13003/annotation943480/range943480/1
type: Range
persistent identity: range943480
display id: range943480
version: 1
direction: direction/forward
top level: BBa_I13003
start: 20
end: 742
orientation: inline

BBa_K1114109
type: ComponentDefinition
persistent identity: BBa K1114109
display id: BBa_K1114109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic design RBS 12, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: MoClo Fusion Site C
sequence annotation: MoClo Fusion Site B
sequence annotation: BCD12
sequence: https://synbiohub.org/public/igem/BBa_K1114109_sequence/1

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361877
display id: annotation2361877
version: 1
top level: BBa_K1114109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114109/annotation2361877/range2361877/1

https://synbiohub.org/public/igem/BBa_K1114109/annotation2361877/range2361877/1
type: Range
persistent identity: range2361877
display id: range2361877
version: 1
direction: direction/forward
top level: BBa_K1114109
start: 89
end: 92
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361876
display id: annotation2361876
version: 1
top level: BBa_K1114109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114109/annotation2361876/range2361876/1

https://synbiohub.org/public/igem/BBa_K1114109/annotation2361876/range2361876/1
type: Range
persistent identity: range2361876
display id: range2361876
version: 1
direction: direction/forward
top level: BBa_K1114109
start: 1
end: 4
orientation: inline

BCD12
type: SequenceAnnotation
persistent identity: annotation2361880
display id: annotation2361880
version: 1
top level: BBa_K1114109
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114109/annotation2361880/range2361880/1

https://synbiohub.org/public/igem/BBa_K1114109/annotation2361880/range2361880/1
type: Range
persistent identity: range2361880
display id: range2361880
version: 1
direction: direction/forward
top level: BBa_K1114109
start: 5
end: 88
orientation: inline

BBa_K1033928
type: ComponentDefinition
persistent identity: BBa K1033928
display id: BBa_K1033928
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue, blue chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.
mutable notes: Synthesized and codon optimized for E. coli by Bioneer Corp.
mutable provenance: Actinia equina
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23110
component: BBa_K864401
component: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_K864401
sequence: https://synbiohub.org/public/igem/BBa_K1033928_sequence/1

BBa_J23110
type: Component
persistent identity: component2351882
display id: component2351882
version: 1
top level: BBa_K1033928
access: public
definition: BBa_J23110

BBa_K864401
type: Component
persistent identity: component2351886
display id: component2351886
version: 1
top level: BBa_K1033928
access: public
definition: https://synbiohub.org/public/igem/BBa_K864401/1

BBa_B0034
type: Component
persistent identity: component2351884
display id: component2351884
version: 1
top level: BBa_K1033928
access: public
definition: BBa_B0034

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2351882
display id: annotation2351882
version: 1
top level: BBa_K1033928
location: https://synbiohub.org/public/igem/BBa_K1033928/annotation2351882/range2351882/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033928/annotation2351882/range2351882/1
type: Range
persistent identity: range2351882
display id: range2351882
version: 1
direction: direction/reverse
top level: BBa_K1033928
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351884
display id: annotation2351884
version: 1
top level: BBa_K1033928
location: https://synbiohub.org/public/igem/BBa_K1033928/annotation2351884/range2351884/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033928/annotation2351884/range2351884/1
type: Range
persistent identity: range2351884
display id: range2351884
version: 1
direction: direction/reverse
top level: BBa_K1033928
start: 44
end: 55
orientation: inline

BBa_K864401
type: SequenceAnnotation
persistent identity: annotation2351886
display id: annotation2351886
version: 1
top level: BBa_K1033928
location: https://synbiohub.org/public/igem/BBa_K1033928/annotation2351886/range2351886/1
component: BBa_K864401

https://synbiohub.org/public/igem/BBa_K1033928/annotation2351886/range2351886/1
type: Range
persistent identity: range2351886
display id: range2351886
version: 1
direction: direction/reverse
top level: BBa_K1033928
start: 62
end: 760
orientation: inline

BBa_S03119
type: ComponentDefinition
persistent identity: BBa S03119
display id: BBa_S03119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: --Specify Parts List--
creator: Randy Rettberg
created: 2004-04-19T11:00:00Z
modified: 2015-05-08T01:14:19Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from assembly 299
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_C0062
component: BBa_B0010
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03119_sequence/1

BBa_B0012
type: Component
persistent identity: component955067
display id: component955067
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component955049
display id: component955049
version: 1
top level: BBa_S03119
access: public
definition: luxr

BBa_B0010
type: Component
persistent identity: component955057
display id: component955057
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component955034
display id: component955034
version: 1
top level: BBa_S03119
access: public
definition: BBa_B0034

BBa_R0040
type: Component
persistent identity: component955026
display id: component955026
version: 1
top level: BBa_S03119
access: public
definition: p(tetR)

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation955057
display id: annotation955057
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955057/range955057/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03119/annotation955057/range955057/1
type: Range
persistent identity: range955057
display id: range955057
version: 1
direction: direction/forward
top level: BBa_S03119
start: 870
end: 949
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation955067
display id: annotation955067
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955067/range955067/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03119/annotation955067/range955067/1
type: Range
persistent identity: range955067
display id: range955067
version: 1
direction: direction/forward
top level: BBa_S03119
start: 958
end: 998
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation955049
display id: annotation955049
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955049/range955049/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_S03119/annotation955049/range955049/1
type: Range
persistent identity: range955049
display id: range955049
version: 1
direction: direction/forward
top level: BBa_S03119
start: 81
end: 836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation955026
display id: annotation955026
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955026/range955026/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03119/annotation955026/range955026/1
type: Range
persistent identity: range955026
display id: range955026
version: 1
direction: direction/forward
top level: BBa_S03119
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation955034
display id: annotation955034
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955034/range955034/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03119/annotation955034/range955034/1
type: Range
persistent identity: range955034
display id: range955034
version: 1
direction: direction/forward
top level: BBa_S03119
start: 63
end: 74
orientation: inline

BBa_I13601
type: ComponentDefinition
persistent identity: BBa I13601
display id: BBa_I13601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac operator with CFP reporter (without LVA tag) [R/Lc-]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0011
component: BBa_E0420
sequence annotation: BBa_R0011
sequence annotation: BBa_E0420
sequence: https://synbiohub.org/public/igem/BBa_I13601_sequence/1

BBa_R0011
type: Component
persistent identity: component2221463
display id: component2221463
version: 1
top level: BBa_I13601
access: public
definition: lacI+pL

BBa_E0420
type: Component
persistent identity: component2221477
display id: component2221477
version: 1
top level: BBa_I13601
access: public
definition: BBa_E0420

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221463
display id: annotation2221463
version: 1
top level: BBa_I13601
location: https://synbiohub.org/public/igem/BBa_I13601/annotation2221463/range2221463/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13601/annotation2221463/range2221463/1
type: Range
persistent identity: range2221463
display id: range2221463
version: 1
direction: direction/forward
top level: BBa_I13601
start: 1
end: 54
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2221477
display id: annotation2221477
version: 1
top level: BBa_I13601
location: https://synbiohub.org/public/igem/BBa_I13601/annotation2221477/range2221477/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13601/annotation2221477/range2221477/1
type: Range
persistent identity: range2221477
display id: range2221477
version: 1
direction: direction/reverse
top level: BBa_I13601
start: 64
end: 941
orientation: inline

BBa_K1114100
type: ComponentDefinition
persistent identity: BBa K1114100
display id: BBa_K1114100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo format of a modified version of BBa_B0030 with BC fusion sites.
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a modified BBa_B0030 with BC fusion sites. See the BBa_R0010 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_B0030???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of a modified BBa_B0030 with BC fusion sites. See the BBa_R0010 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_B0030???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114100_sequence/1

BBa_K352002
type: ComponentDefinition
persistent identity: BBa K352002
display id: BBa_K352002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCooF Promoter from Rhodospirillum rubrum
creator: Ozkan IS
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
group u list: _474_
m user id: 0
owner id: 5815
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CooA is a CO-sensing protein that activates the transcription of genes encoding the CO-oxidation (coo) regulon, whose polypeptide products are required for utilizing CO as an energy source in Rhodospirillum rubrum. CooA binds to a position overlapping the -35 element of the P(cooF) promoter, similar to the arrangement of class II CRP (cAMP receptor protein)- and FNR (fumarate and nitrate reductase activator protein)-dependent promoters when expressed in Escherichia coli. The CO-dependent anaerobic growth of Rhodospirillum rubrum relies on a CO oxidation system encoded by two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ. The key products of the coo regulon are an O2- sensitive CO dehydrogenase (CooS), a CooS-associated Fe-S protein (CooF), and a CO-tolerant hydrogenase (CooH), and the expression of the genes depends upon the activity of the CooA protein, which senses CO under anaerobic conditions. CO-independent basal level transcription of PcooF is not detectable due to the absence of active CooA.
mutable notes: The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.
mutable provenance: S.W. Singer, M.B. Hirst and P.W. Ludden, CO-dependent H2 evolution by Rhodospirillum rubrum: role of CODH:CooF complex, Biochim Biophys Acta - Bioenerg 1757 (2006), pp. 1582???1591
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Protected by CooA
sequence annotation: CooA binding site
sequence annotation: A+T rich sequence
sequence annotation: Transcription Start
sequence annotation: Protected by CooA+RNAP
sequence annotation: -10 Region
sequence: https://synbiohub.org/public/igem/BBa_K352002_sequence/1

Protected by CooA
type: SequenceAnnotation
persistent identity: annotation2081257
display id: annotation2081257
version: 1
top level: BBa_K352002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K352002/annotation2081257/range2081257/1

https://synbiohub.org/public/igem/BBa_K352002/annotation2081257/range2081257/1
type: Range
persistent identity: range2081257
display id: range2081257
version: 1
direction: direction/forward
top level: BBa_K352002
start: 36
end: 61
orientation: inline

CooA binding site
type: SequenceAnnotation
persistent identity: annotation2081253
display id: annotation2081253
version: 1
top level: BBa_K352002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K352002/annotation2081253/range2081253/1

https://synbiohub.org/public/igem/BBa_K352002/annotation2081253/range2081253/1
type: Range
persistent identity: range2081253
display id: range2081253
version: 1
direction: direction/forward
top level: BBa_K352002
start: 41
end: 44
orientation: inline

A+T rich sequence
type: SequenceAnnotation
persistent identity: annotation2081252
display id: annotation2081252
version: 1
top level: BBa_K352002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K352002/annotation2081252/range2081252/1

https://synbiohub.org/public/igem/BBa_K352002/annotation2081252/range2081252/1
type: Range
persistent identity: range2081252
display id: range2081252
version: 1
direction: direction/forward
top level: BBa_K352002
start: 8
end: 33
orientation: inline

CooA binding site
type: SequenceAnnotation
persistent identity: annotation2081254
display id: annotation2081254
version: 1
top level: BBa_K352002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K352002/annotation2081254/range2081254/1

https://synbiohub.org/public/igem/BBa_K352002/annotation2081254/range2081254/1
type: Range
persistent identity: range2081254
display id: range2081254
version: 1
direction: direction/forward
top level: BBa_K352002
start: 52
end: 56
orientation: inline

Transcription Start
type: SequenceAnnotation
persistent identity: annotation2081256
display id: annotation2081256
version: 1
top level: BBa_K352002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K352002/annotation2081256/range2081256/1

https://synbiohub.org/public/igem/BBa_K352002/annotation2081256/range2081256/1
type: Range
persistent identity: range2081256
display id: range2081256
version: 1
direction: direction/forward
top level: BBa_K352002
start: 92
end: 93
orientation: inline

Protected by CooA+RNAP
type: SequenceAnnotation
persistent identity: annotation2081258
display id: annotation2081258
version: 1
top level: BBa_K352002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K352002/annotation2081258/range2081258/1

https://synbiohub.org/public/igem/BBa_K352002/annotation2081258/range2081258/1
type: Range
persistent identity: range2081258
display id: range2081258
version: 1
direction: direction/forward
top level: BBa_K352002
start: 23
end: 91
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation2081255
display id: annotation2081255
version: 1
top level: BBa_K352002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K352002/annotation2081255/range2081255/1

https://synbiohub.org/public/igem/BBa_K352002/annotation2081255/range2081255/1
type: Range
persistent identity: range2081255
display id: range2081255
version: 1
direction: direction/forward
top level: BBa_K352002
start: 79
end: 84
orientation: inline

BBa_K622006
type: ComponentDefinition
persistent identity: BBa K622006
display id: BBa_K622006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ChitinaseA1
creator: Fumitaka Hashiya
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
group u list: _794_
m user id: 0
owner id: 9320
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes ChiA1 and its RBS. Its start codon is not AUG but GUG.
mutable notes: Start codon of this part is GUG.
mutable provenance: Genomic DNA of Streptomyces avermitilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K622006_sequence/1

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR repressor/activator, (no LVA?)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: BBa_C0062
sequence annotation: A
sequence annotation: Help:Barcodes
sequence annotation: prefix
sequence annotation: T
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 750
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 756
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
direction: direction/forward
top level: luxr
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
direction: direction/forward
top level: luxr
start: 757
end: 781
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 2
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
direction: direction/forward
top level: luxr
start: 174
end: 174
orientation: inline

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

cinR
type: ComponentDefinition
persistent identity: BBa R0077
display id: BBa_R0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated, RBS+)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription. This promoter contains the RBS: TGGAGG
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence annotation: embedded RBS
sequence: https://synbiohub.org/public/igem/BBa_R0077_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation301371
display id: annotation301371
version: 1
top level: cinR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1
type: Range
persistent identity: range301371
display id: range301371
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301205
display id: annotation301205
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1
type: Range
persistent identity: range301205
display id: range301205
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301204
display id: annotation301204
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1
type: Range
persistent identity: range301204
display id: range301204
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

embedded RBS
type: SequenceAnnotation
persistent identity: annotation301150
display id: annotation301150
version: 1
top level: cinR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1
type: Range
persistent identity: range301150
display id: range301150
version: 1
direction: direction/forward
top level: cinR
start: 226
end: 231
orientation: inline

spisPink
type: ComponentDefinition
persistent identity: BBa K1033925
display id: BBa_K1033925
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, pink chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Stylophora pistillata, spisPink, naturally exhibits color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Stylophora pistillata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: spisPink
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1033925_sequence/1

spisPink
type: SequenceAnnotation
persistent identity: annotation2351831
display id: annotation2351831
version: 1
top level: spisPink
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033925/annotation2351831/range2351831/1

https://synbiohub.org/public/igem/BBa_K1033925/annotation2351831/range2351831/1
type: Range
persistent identity: range2351831
display id: range2351831
version: 1
direction: direction/forward
top level: spisPink
start: 20
end: 694
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2351830
display id: annotation2351830
version: 1
top level: spisPink
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033925/annotation2351830/range2351830/1

https://synbiohub.org/public/igem/BBa_K1033925/annotation2351830/range2351830/1
type: Range
persistent identity: range2351830
display id: range2351830
version: 1
direction: direction/forward
top level: spisPink
start: 5
end: 13
orientation: inline

BBa_K729002
type: ComponentDefinition
persistent identity: BBa K729002
display id: BBa_K729002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Laccase for Polyethylene Degradation
creator: Bouran Sohrabi
created: 2012-06-26T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _975_
m user id: 0
owner id: 11516
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: Genomic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K729002_sequence/1

BBa_K1390001
type: ComponentDefinition
persistent identity: BBa K1390001
display id: BBa_K1390001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mmoB
creator: Stefan D??bel
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:13Z
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mmoB is one subunit of the mmo from Methylococcus capsulatus bath
mutable notes: the brick is constructed in the RCF10 standard
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390001_sequence/1

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0022
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
access: public
definition: ECFP

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
direction: direction/forward
top level: BBa_E0422
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
direction: direction/forward
top level: BBa_E0422
start: 19
end: 780
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
direction: direction/forward
top level: BBa_E0422
start: 877
end: 917
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
direction: direction/forward
top level: BBa_E0422
start: 789
end: 868
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: luxI
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
direction: direction/forward
top level: luxI
start: 580
end: 611
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 579
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
direction: direction/forward
top level: luxI
start: 619
end: 643
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 618
orientation: inline

BBa_I13001
type: ComponentDefinition
persistent identity: BBa I13001
display id: BBa_I13001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS B0030 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0030. E0030. B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I13001_sequence/1

BBa_B0030
type: Component
persistent identity: component943428
display id: component943428
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component943446
display id: component943446
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943436
display id: component943436
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component943431
display id: component943431
version: 1
top level: BBa_I13001
access: public
definition: eyfp

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943431
display id: annotation943431
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943431/range943431/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13001/annotation943431/range943431/1
type: Range
persistent identity: range943431
display id: range943431
version: 1
direction: direction/forward
top level: BBa_I13001
start: 22
end: 744
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943446
display id: annotation943446
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943446/range943446/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13001/annotation943446/range943446/1
type: Range
persistent identity: range943446
display id: range943446
version: 1
direction: direction/forward
top level: BBa_I13001
start: 841
end: 881
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943436
display id: annotation943436
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943436/range943436/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13001/annotation943436/range943436/1
type: Range
persistent identity: range943436
display id: range943436
version: 1
direction: direction/forward
top level: BBa_I13001
start: 753
end: 832
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation943428
display id: annotation943428
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943428/range943428/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I13001/annotation943428/range943428/1
type: Range
persistent identity: range943428
display id: range943428
version: 1
direction: direction/forward
top level: BBa_I13001
start: 1
end: 15
orientation: inline

tsPurple
type: ComponentDefinition
persistent identity: BBa K1033906
display id: BBa_K1033906
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple, purple chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from TinselPurple vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tsPurple
sequence: https://synbiohub.org/public/igem/BBa_K1033906_sequence/1

tsPurple
type: SequenceAnnotation
persistent identity: annotation2332100
display id: annotation2332100
version: 1
top level: tsPurple
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033906/annotation2332100/range2332100/1

https://synbiohub.org/public/igem/BBa_K1033906/annotation2332100/range2332100/1
type: Range
persistent identity: range2332100
display id: range2332100
version: 1
direction: direction/forward
top level: tsPurple
start: 1
end: 690
orientation: inline

BBa_K1433005
type: ComponentDefinition
persistent identity: BBa K1433005
display id: BBa_K1433005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-gamma protein-beta protein-exonuclease
creator: Hanqing Liu
created: 2014-09-27T11:00:00Z
modified: 2015-05-08T01:10:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1811_
m user id: 0
owner id: 20519
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is constructed for improve BBa_K1223012, pKD46 functional unit. Lambda red & pKD46 brief introduction. BBa_K1223012 is not conform to any RFC cause there are three restrict enzyme sites of EcoRI and PstI. As for improve the compatibility of this part and make these useful modules be used in future assemblies, we remove these restrict enzyme sites by synonymous mutations
mutable notes: No
mutable provenance: No
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: EXO-CDS
sequence annotation: Beta-CDS
sequence annotation: T to G(removing Pst1 site)
sequence annotation: GAM-CDS
sequence annotation: G to A(removing Pst1 site)
sequence annotation: A to G(removing EcoR1 site)
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1433005_sequence/1

EXO-CDS
type: SequenceAnnotation
persistent identity: annotation2412289
display id: annotation2412289
version: 1
top level: BBa_K1433005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1433005/annotation2412289/range2412289/1

https://synbiohub.org/public/igem/BBa_K1433005/annotation2412289/range2412289/1
type: Range
persistent identity: range2412289
display id: range2412289
version: 1
direction: direction/forward
top level: BBa_K1433005
start: 1224
end: 1904
orientation: inline

Beta-CDS
type: SequenceAnnotation
persistent identity: annotation2412279
display id: annotation2412279
version: 1
top level: BBa_K1433005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1433005/annotation2412279/range2412279/1

https://synbiohub.org/public/igem/BBa_K1433005/annotation2412279/range2412279/1
type: Range
persistent identity: range2412279
display id: range2412279
version: 1
direction: direction/forward
top level: BBa_K1433005
start: 442
end: 1227
orientation: inline

T to G(removing Pst1 site)
type: SequenceAnnotation
persistent identity: annotation2412290
display id: annotation2412290
version: 1
top level: BBa_K1433005
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1433005/annotation2412290/range2412290/1

https://synbiohub.org/public/igem/BBa_K1433005/annotation2412290/range2412290/1
type: Range
persistent identity: range2412290
display id: range2412290
version: 1
direction: direction/forward
top level: BBa_K1433005
start: 996
end: 996
orientation: inline

GAM-CDS
type: SequenceAnnotation
persistent identity: annotation2412278
display id: annotation2412278
version: 1
top level: BBa_K1433005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1433005/annotation2412278/range2412278/1

https://synbiohub.org/public/igem/BBa_K1433005/annotation2412278/range2412278/1
type: Range
persistent identity: range2412278
display id: range2412278
version: 1
direction: direction/forward
top level: BBa_K1433005
start: 20
end: 436
orientation: inline

G to A(removing Pst1 site)
type: SequenceAnnotation
persistent identity: annotation2412291
display id: annotation2412291
version: 1
top level: BBa_K1433005
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1433005/annotation2412291/range2412291/1

https://synbiohub.org/public/igem/BBa_K1433005/annotation2412291/range2412291/1
type: Range
persistent identity: range2412291
display id: range2412291
version: 1
direction: direction/forward
top level: BBa_K1433005
start: 1247
end: 1247
orientation: inline

A to G(removing EcoR1 site)
type: SequenceAnnotation
persistent identity: annotation2412292
display id: annotation2412292
version: 1
top level: BBa_K1433005
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1433005/annotation2412292/range2412292/1

https://synbiohub.org/public/igem/BBa_K1433005/annotation2412292/range2412292/1
type: Range
persistent identity: range2412292
display id: range2412292
version: 1
direction: direction/forward
top level: BBa_K1433005
start: 1502
end: 1502
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2412277
display id: annotation2412277
version: 1
top level: BBa_K1433005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1433005/annotation2412277/range2412277/1

https://synbiohub.org/public/igem/BBa_K1433005/annotation2412277/range2412277/1
type: Range
persistent identity: range2412277
display id: range2412277
version: 1
direction: direction/forward
top level: BBa_K1433005
start: 1
end: 19
orientation: inline

BBa_J23112
type: ComponentDefinition
persistent identity: BBa J23112
display id: BBa_J23112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23112_sequence/1

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
mutable provenance: AF196486 from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0060
sequence annotation: LVA
sequence annotation: 2
sequence annotation: aiiA
sequence annotation: start
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 789
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
direction: direction/forward
top level: aiiA
start: 751
end: 783
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
direction: direction/forward
top level: aiiA
start: 784
end: 789
orientation: inline

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 750
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 3
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
direction: direction/forward
top level: aiiA
start: 790
end: 814
orientation: inline

BBa_K1033923
type: ComponentDefinition
persistent identity: BBa K1033923
display id: BBa_K1033923
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, pink chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Stylophora pistillata, spisPink, naturally exhibits color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Stylophora pistillata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033925
component: BBa_J23110
sequence annotation: BBa_K1033925
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K1033923_sequence/1

BBa_K1033925
type: Component
persistent identity: component2371236
display id: component2371236
version: 1
top level: BBa_K1033923
access: public
definition: spisPink

BBa_J23110
type: Component
persistent identity: component2371233
display id: component2371233
version: 1
top level: BBa_K1033923
access: public
definition: BBa_J23110

BBa_K1033925
type: SequenceAnnotation
persistent identity: annotation2371236
display id: annotation2371236
version: 1
top level: BBa_K1033923
location: https://synbiohub.org/public/igem/BBa_K1033923/annotation2371236/range2371236/1
component: BBa_K1033925

https://synbiohub.org/public/igem/BBa_K1033923/annotation2371236/range2371236/1
type: Range
persistent identity: range2371236
display id: range2371236
version: 1
direction: direction/reverse
top level: BBa_K1033923
start: 44
end: 740
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2371233
display id: annotation2371233
version: 1
top level: BBa_K1033923
location: https://synbiohub.org/public/igem/BBa_K1033923/annotation2371233/range2371233/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033923/annotation2371233/range2371233/1
type: Range
persistent identity: range2371233
display id: range2371233
version: 1
direction: direction/reverse
top level: BBa_K1033923
start: 1
end: 35
orientation: inline

BBa_K1424004
type: ComponentDefinition
persistent identity: BBa K1424004
display id: BBa_K1424004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glucose oxidase codon optimised for Synechocystis
creator: Jacob Lamb
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
group u list: _1802_
m user id: 0
owner id: 20667
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Glucose oxidase gene codon optimised for Synechocystis sp. PCC 6803
mutable notes: n/a
mutable provenance: Synthesised gene product
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Codon-optimized glucose oxidase
sequence: https://synbiohub.org/public/igem/BBa_K1424004_sequence/1

Codon-optimized glucose oxidase
type: SequenceAnnotation
persistent identity: annotation2400766
display id: annotation2400766
version: 1
top level: BBa_K1424004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1424004/annotation2400766/range2400766/1

https://synbiohub.org/public/igem/BBa_K1424004/annotation2400766/range2400766/1
type: Range
persistent identity: range2400766
display id: range2400766
version: 1
direction: direction/forward
top level: BBa_K1424004
start: 1
end: 1818
orientation: inline

BBa_I13452
type: ComponentDefinition
persistent identity: BBa I13452
display id: BBa_I13452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Adds another BioBrick site
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.
mutable notes: 70 bp from Eco to Spe or Xba to Pst, to allow for gel extraction. The middle portion is largely noise, intended as filler to raise the size of the part.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Future Pst site
sequence annotation: BbsI site
sequence annotation: BstXI site
sequence annotation: Future Eco site
sequence: https://synbiohub.org/public/igem/BBa_I13452_sequence/1

Future Pst site
type: SequenceAnnotation
persistent identity: annotation1891622
display id: annotation1891622
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1
type: Range
persistent identity: range1891622
display id: range1891622
version: 1
direction: direction/forward
top level: BBa_I13452
start: 44
end: 49
orientation: inline

BbsI site
type: SequenceAnnotation
persistent identity: annotation1891620
display id: annotation1891620
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1
type: Range
persistent identity: range1891620
display id: range1891620
version: 1
direction: direction/forward
top level: BBa_I13452
start: 8
end: 13
orientation: inline

BstXI site
type: SequenceAnnotation
persistent identity: annotation1891621
display id: annotation1891621
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1
type: Range
persistent identity: range1891621
display id: range1891621
version: 1
direction: direction/forward
top level: BBa_I13452
start: 41
end: 52
orientation: inline

Future Eco site
type: SequenceAnnotation
persistent identity: annotation1891619
display id: annotation1891619
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1
type: Range
persistent identity: range1891619
display id: range1891619
version: 1
direction: direction/forward
top level: BBa_I13452
start: 1
end: 6
orientation: inline

BBa_K1114025
type: ComponentDefinition
persistent identity: BBa K1114025
display id: BBa_K1114025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_R0040 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_R0040 with AB fusion sites. See the BBa_R0040 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_R0040 with AB fusion sites. See the BBa_R0040 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114025_sequence/1

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
component: BBa_B0012
component: BBa_B0010
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: BBa_B0034

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_K1321334
type: ComponentDefinition
persistent identity: BBa K1321334
display id: BBa_K1321334
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB-AcsAB
creator: Laura de Arroyo Garcia
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 21121
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is made up of the coding sequence of the A and B elements of the acs, cellulose-producing operon. AcsA and acsB are transcribed and translated in the form of a single polypeptide, and form the main two domains (regulatory and catalytic) of the Cellulose Synthase enzyme in Gluconacetobacter Xylinus.
mutable notes: The Ribosomal binding site of AcsAB is already included within the sequence. Because we are transferring this element from Gluconacetobacter into simpler organisms, such as E.coli, the native RBS was replaced with a new RBS, that would guarantee strong translation whilst maintaining the native stoichiometry for the final operon system to operate successfully.
mutable provenance: The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Start Codon
sequence annotation: AcsAB Coding Sequence
sequence annotation: RBS - AcsAB
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K1321334_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2428734
display id: annotation2428734
version: 1
top level: BBa_K1321334
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1321334/annotation2428734/range2428734/1

https://synbiohub.org/public/igem/BBa_K1321334/annotation2428734/range2428734/1
type: Range
persistent identity: range2428734
display id: range2428734
version: 1
direction: direction/forward
top level: BBa_K1321334
start: 68
end: 71
orientation: inline

AcsAB Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2428059
display id: annotation2428059
version: 1
top level: BBa_K1321334
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321334/annotation2428059/range2428059/1

https://synbiohub.org/public/igem/BBa_K1321334/annotation2428059/range2428059/1
type: Range
persistent identity: range2428059
display id: range2428059
version: 1
direction: direction/forward
top level: BBa_K1321334
start: 68
end: 4678
orientation: inline

RBS - AcsAB
type: SequenceAnnotation
persistent identity: annotation2428058
display id: annotation2428058
version: 1
top level: BBa_K1321334
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1321334/annotation2428058/range2428058/1

https://synbiohub.org/public/igem/BBa_K1321334/annotation2428058/range2428058/1
type: Range
persistent identity: range2428058
display id: range2428058
version: 1
direction: direction/forward
top level: BBa_K1321334
start: 28
end: 51
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2428733
display id: annotation2428733
version: 1
top level: BBa_K1321334
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1321334/annotation2428733/range2428733/1

https://synbiohub.org/public/igem/BBa_K1321334/annotation2428733/range2428733/1
type: Range
persistent identity: range2428733
display id: range2428733
version: 1
direction: direction/forward
top level: BBa_K1321334
start: 4679
end: 4684
orientation: inline

BBa_K1033913
type: ComponentDefinition
persistent identity: BBa K1033913
display id: BBa_K1033913
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: scOrange, orange chromoprotein (incl. RBS)
creator: Erik Gullberg
created: 2013-08-25T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from ScroogeOrange vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS
sequence annotation: BBa_K1033903
sequence: https://synbiohub.org/public/igem/BBa_K1033913_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2332148
display id: annotation2332148
version: 1
top level: BBa_K1033913
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033913/annotation2332148/range2332148/1

https://synbiohub.org/public/igem/BBa_K1033913/annotation2332148/range2332148/1
type: Range
persistent identity: range2332148
display id: range2332148
version: 1
direction: direction/forward
top level: BBa_K1033913
start: 1
end: 12
orientation: inline

BBa_K1033903
type: SequenceAnnotation
persistent identity: annotation2332149
display id: annotation2332149
version: 1
top level: BBa_K1033913
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033913/annotation2332149/range2332149/1

https://synbiohub.org/public/igem/BBa_K1033913/annotation2332149/range2332149/1
type: Range
persistent identity: range2332149
display id: range2332149
version: 1
direction: direction/forward
top level: BBa_K1033913
start: 19
end: 714
orientation: inline

BBa_K1357010
type: ComponentDefinition
persistent identity: BBa K1357010
display id: BBa_K1357010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eRFP reporter
creator: Matthew Tucker
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _1733_
m user id: 0
owner id: 16772
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS + Coding + Term
mutable notes: None
mutable provenance: jellyfish
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E1010
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1357010_sequence/1

BBa_B0034
type: Component
persistent identity: component2408713
display id: component2408713
version: 1
top level: BBa_K1357010
access: public
definition: BBa_B0034

BBa_E1010
type: Component
persistent identity: component2408716
display id: component2408716
version: 1
top level: BBa_K1357010
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0015
type: Component
persistent identity: component2408723
display id: component2408723
version: 1
top level: BBa_K1357010
access: public
definition: BBa_B0015

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2408713
display id: annotation2408713
version: 1
top level: BBa_K1357010
location: https://synbiohub.org/public/igem/BBa_K1357010/annotation2408713/range2408713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1357010/annotation2408713/range2408713/1
type: Range
persistent identity: range2408713
display id: range2408713
version: 1
direction: direction/reverse
top level: BBa_K1357010
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2408723
display id: annotation2408723
version: 1
top level: BBa_K1357010
location: https://synbiohub.org/public/igem/BBa_K1357010/annotation2408723/range2408723/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1357010/annotation2408723/range2408723/1
type: Range
persistent identity: range2408723
display id: range2408723
version: 1
direction: direction/reverse
top level: BBa_K1357010
start: 733
end: 861
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2408716
display id: annotation2408716
version: 1
top level: BBa_K1357010
location: https://synbiohub.org/public/igem/BBa_K1357010/annotation2408716/range2408716/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1357010/annotation2408716/range2408716/1
type: Range
persistent identity: range2408716
display id: range2408716
version: 1
direction: direction/reverse
top level: BBa_K1357010
start: 19
end: 724
orientation: inline

BBa_K783028
type: ComponentDefinition
persistent identity: BBa K783028
display id: BBa_K783028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_J23110
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_J23110. It has two MoClo fusion sites flanking this part.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_J23110
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K783028_sequence/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2214228
display id: annotation2214228
version: 1
top level: BBa_K783028
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K783028/annotation2214228/range2214228/1

https://synbiohub.org/public/igem/BBa_K783028/annotation2214228/range2214228/1
type: Range
persistent identity: range2214228
display id: range2214228
version: 1
direction: direction/reverse
top level: BBa_K783028
start: 1
end: 35
orientation: inline

BBa_K729004
type: ComponentDefinition
persistent identity: BBa K729004
display id: BBa_K729004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nuclease from Staphylococcus aureus
creator: Bouran Sohrabi
created: 2012-06-29T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _975_
m user id: 0
owner id: 11516
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Nuclease from Staphylococcus aureus. Sequence pending.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K729004_sequence/1

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_K1033915
type: ComponentDefinition
persistent identity: BBa K1033915
display id: BBa_K1033915
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia majano
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033916
component: BBa_B0034
sequence annotation: BBa_K1033916
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033915_sequence/1

BBa_K1033916
type: Component
persistent identity: component2350102
display id: component2350102
version: 1
top level: BBa_K1033915
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033916/1

BBa_B0034
type: Component
persistent identity: component2350100
display id: component2350100
version: 1
top level: BBa_K1033915
access: public
definition: BBa_B0034

BBa_K1033916
type: SequenceAnnotation
persistent identity: annotation2350102
display id: annotation2350102
version: 1
top level: BBa_K1033915
location: https://synbiohub.org/public/igem/BBa_K1033915/annotation2350102/range2350102/1
component: BBa_K1033916

https://synbiohub.org/public/igem/BBa_K1033915/annotation2350102/range2350102/1
type: Range
persistent identity: range2350102
display id: range2350102
version: 1
direction: direction/reverse
top level: BBa_K1033915
start: 19
end: 711
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2350100
display id: annotation2350100
version: 1
top level: BBa_K1033915
location: https://synbiohub.org/public/igem/BBa_K1033915/annotation2350100/range2350100/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033915/annotation2350100/range2350100/1
type: Range
persistent identity: range2350100
display id: range2350100
version: 1
direction: direction/reverse
top level: BBa_K1033915
start: 1
end: 12
orientation: inline

BBa_K1114018
type: ComponentDefinition
persistent identity: BBa K1114018
display id: BBa_K1114018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23116 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_J23116 with AB fusion sites. See the BBa_J23116 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_J23116 with AB fusion sites. See the BBa_J23116 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114018_sequence/1

BBa_K1073024
type: ComponentDefinition
persistent identity: BBa K1073024
display id: BBa_K1073024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutively expressed chromoprotein amilGFP
creator: iGEM Team Braunschweig 2013
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The chromoprotein amilGFP is constitutively expressed and can be used for the marking of cells for easier differentiation.
mutable notes: no considerations.
mutable provenance: This part is a combination of BBa_K1073006 and BBa_K592010, which were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592010
component: BBa_K1073006
sequence annotation: BBa_K592010
sequence annotation: BBa_K1073006
sequence: https://synbiohub.org/public/igem/BBa_K1073024_sequence/1

BBa_K592010
type: Component
persistent identity: component2354086
display id: component2354086
version: 1
top level: BBa_K1073024
access: public
definition: https://synbiohub.org/public/igem/BBa_K592010/1

BBa_K1073006
type: Component
persistent identity: component2354084
display id: component2354084
version: 1
top level: BBa_K1073024
access: public
definition: https://synbiohub.org/public/igem/BBa_K1073006/1

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2354086
display id: annotation2354086
version: 1
top level: BBa_K1073024
location: https://synbiohub.org/public/igem/BBa_K1073024/annotation2354086/range2354086/1
component: BBa_K592010

https://synbiohub.org/public/igem/BBa_K1073024/annotation2354086/range2354086/1
type: Range
persistent identity: range2354086
display id: range2354086
version: 1
direction: direction/reverse
top level: BBa_K1073024
start: 63
end: 761
orientation: inline

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354084
display id: annotation2354084
version: 1
top level: BBa_K1073024
location: https://synbiohub.org/public/igem/BBa_K1073024/annotation2354084/range2354084/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073024/annotation2354084/range2354084/1
type: Range
persistent identity: range2354084
display id: range2354084
version: 1
direction: direction/reverse
top level: BBa_K1073024
start: 1
end: 56
orientation: inline

BBa_K1650037
type: ComponentDefinition
persistent identity: BBa K1650037
display id: BBa_K1650037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpyCatcher
creator: Alexandra Richter
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:03:58Z
discontinued: false
dominant: false
group u list: _2067_
m user id: 12520
owner id: 27246
owning group id: 9
status: status/Planning
bookmark: false
mutable description: part of the SpySystem
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1650037
sequence: https://synbiohub.org/public/igem/BBa_K1650037_sequence/1

BBa_K1650037
type: SequenceAnnotation
persistent identity: annotation2474920
display id: annotation2474920
version: 1
top level: BBa_K1650037
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1650037/annotation2474920/range2474920/1

https://synbiohub.org/public/igem/BBa_K1650037/annotation2474920/range2474920/1
type: Range
persistent identity: range2474920
display id: range2474920
version: 1
direction: direction/forward
top level: BBa_K1650037
start: 1
end: 417
orientation: inline

BBa_P0151
type: ComponentDefinition
persistent identity: BBa P0151
display id: BBa_P0151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (lambda) [S0165]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from lambda. Used as the input section for Quad Part Inverters Q01150 and Q01151.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0051
component: BBa_B0010
component: BBa_B0012
component: BBa_B0031
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_P0151_sequence/1

BBa_C0051
type: Component
persistent identity: component944513
display id: component944513
version: 1
top level: BBa_P0151
access: public
definition: cI lam

BBa_B0010
type: Component
persistent identity: component944519
display id: component944519
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component944529
display id: component944529
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: Component
persistent identity: component944503
display id: component944503
version: 1
top level: BBa_P0151
access: public
definition: BBa_B0031

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944513
display id: annotation944513
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
type: Range
persistent identity: range944513
display id: range944513
version: 1
direction: direction/forward
top level: BBa_P0151
start: 21
end: 770
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944529
display id: annotation944529
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
type: Range
persistent identity: range944529
display id: range944529
version: 1
direction: direction/forward
top level: BBa_P0151
start: 892
end: 932
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944503
display id: annotation944503
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
type: Range
persistent identity: range944503
display id: range944503
version: 1
direction: direction/forward
top level: BBa_P0151
start: 1
end: 14
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944519
display id: annotation944519
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
type: Range
persistent identity: range944519
display id: range944519
version: 1
direction: direction/forward
top level: BBa_P0151
start: 804
end: 883
orientation: inline

BBa_K115017
type: ComponentDefinition
persistent identity: BBa K115017
display id: BBa_K115017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (ROSE 32??C)
creator: Bastiaan van den Berg
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:09:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: An RNA thermometer that theoretically switches on translation at 32 degrees Celcius and switched of translation below that temperature. Still to be tested.
mutable notes: a lot, will be added soon.
mutable provenance: Based on ROSE RNA thermometer sequences from Rfam database.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: SD
sequence: https://synbiohub.org/public/igem/BBa_K115017_sequence/1

SD
type: SequenceAnnotation
persistent identity: annotation1972920
display id: annotation1972920
version: 1
top level: BBa_K115017
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115017/annotation1972920/range1972920/1

https://synbiohub.org/public/igem/BBa_K115017/annotation1972920/range1972920/1
type: Range
persistent identity: range1972920
display id: range1972920
version: 1
direction: direction/forward
top level: BBa_K115017
start: 77
end: 82
orientation: inline

T7pol
type: ComponentDefinition
persistent identity: BBa K145001
display id: BBa_K145001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNA polymerase
creator: Jonas Demeulemeester and Benjamien Moeyaert
created: 2008-07-07T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: T7 RNA polymerase with LVA tag
mutable notes: Fuse the LVA tag to the T7 coding region
mutable provenance: BBa_I712022 LVA tag from BBa_E0022
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K145001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1968216
display id: annotation1968216
version: 1
top level: T7pol
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968216/range1968216/1

https://synbiohub.org/public/igem/BBa_K145001/annotation1968216/range1968216/1
type: Range
persistent identity: range1968216
display id: range1968216
version: 1
direction: direction/forward
top level: T7pol
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968218
display id: annotation1968218
version: 1
top level: T7pol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968218/range1968218/1

https://synbiohub.org/public/igem/BBa_K145001/annotation1968218/range1968218/1
type: Range
persistent identity: range1968218
display id: range1968218
version: 1
direction: direction/forward
top level: T7pol
start: 2653
end: 2655
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation1968243
display id: annotation1968243
version: 1
top level: T7pol
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968243/range1968243/1

https://synbiohub.org/public/igem/BBa_K145001/annotation1968243/range1968243/1
type: Range
persistent identity: range1968243
display id: range1968243
version: 1
direction: direction/forward
top level: T7pol
start: 4
end: 2649
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968217
display id: annotation1968217
version: 1
top level: T7pol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968217/range1968217/1

https://synbiohub.org/public/igem/BBa_K145001/annotation1968217/range1968217/1
type: Range
persistent identity: range1968217
display id: range1968217
version: 1
direction: direction/forward
top level: T7pol
start: 2650
end: 2652
orientation: inline

BBa_E0430
type: ComponentDefinition
persistent identity: BBa E0430
display id: BBa_E0430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
creator: Caitlin Conboy
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Standard YFP Output Device -LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0430_sequence/1

BBa_B0012
type: Component
persistent identity: component942902
display id: component942902
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component942892
display id: component942892
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component942885
display id: component942885
version: 1
top level: BBa_E0430
access: public
definition: BBa_B0034

BBa_E0030
type: Component
persistent identity: component942887
display id: component942887
version: 1
top level: BBa_E0430
access: public
definition: eyfp

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942902
display id: annotation942902
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
type: Range
persistent identity: range942902
display id: range942902
version: 1
direction: direction/forward
top level: BBa_E0430
start: 838
end: 878
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation942887
display id: annotation942887
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
type: Range
persistent identity: range942887
display id: range942887
version: 1
direction: direction/forward
top level: BBa_E0430
start: 19
end: 741
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942885
display id: annotation942885
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
type: Range
persistent identity: range942885
display id: range942885
version: 1
direction: direction/forward
top level: BBa_E0430
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942892
display id: annotation942892
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
type: Range
persistent identity: range942892
display id: range942892
version: 1
direction: direction/forward
top level: BBa_E0430
start: 750
end: 829
orientation: inline

BBa_K1418040
type: ComponentDefinition
persistent identity: BBa K1418040
display id: BBa_K1418040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP
creator: Cody Maxfield
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:10:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1796_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CFP
mutable notes: RFC 23
mutable provenance: CFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: atg
sequence annotation: taataa
sequence annotation: CFP
sequence: https://synbiohub.org/public/igem/BBa_K1418040_sequence/1

atg
type: SequenceAnnotation
persistent identity: annotation2421328
display id: annotation2421328
version: 1
top level: BBa_K1418040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1418040/annotation2421328/range2421328/1

https://synbiohub.org/public/igem/BBa_K1418040/annotation2421328/range2421328/1
type: Range
persistent identity: range2421328
display id: range2421328
version: 1
direction: direction/forward
top level: BBa_K1418040
start: 1
end: 3
orientation: inline

taataa
type: SequenceAnnotation
persistent identity: annotation2421352
display id: annotation2421352
version: 1
top level: BBa_K1418040
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1418040/annotation2421352/range2421352/1

https://synbiohub.org/public/igem/BBa_K1418040/annotation2421352/range2421352/1
type: Range
persistent identity: range2421352
display id: range2421352
version: 1
direction: direction/forward
top level: BBa_K1418040
start: 718
end: 723
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2421329
display id: annotation2421329
version: 1
top level: BBa_K1418040
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1418040/annotation2421329/range2421329/1

https://synbiohub.org/public/igem/BBa_K1418040/annotation2421329/range2421329/1
type: Range
persistent identity: range2421329
display id: range2421329
version: 1
direction: direction/forward
top level: BBa_K1418040
start: 4
end: 717
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Replace later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K1122006
type: ComponentDefinition
persistent identity: BBa K1122006
display id: BBa_K1122006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry (BBa_J06504) compatible with Genabler assembly
creator: Lina Gasiūnaitė
created: 2013-08-12T11:00:00Z
modified: 2016-01-26T02:16:52Z
discontinued: false
dominant: false
group u list: _1433_
m user id: 4206
owner id: 18510
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mCherry (BBa_J0654) part with inward facing EarI recognition sites at the ends compatible with Genabler assembly.
mutable notes: To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_E0040.
mutable provenance: mCherry is based on the BioBrick BBa_J06504.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: mCherry
sequence annotation: EarI site
sequence: https://synbiohub.org/public/igem/BBa_K1122006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2334606
display id: annotation2334606
version: 1
top level: BBa_K1122006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334606/range2334606/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334606/range2334606/1
type: Range
persistent identity: range2334606
display id: range2334606
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 724
end: 726
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2334604
display id: annotation2334604
version: 1
top level: BBa_K1122006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334604/range2334604/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334604/range2334604/1
type: Range
persistent identity: range2334604
display id: range2334604
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 1
end: 3
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2346320
display id: annotation2346320
version: 1
top level: BBa_K1122006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2346320/range2346320/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2346320/range2346320/1
type: Range
persistent identity: range2346320
display id: range2346320
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 1
end: 727
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334605
display id: annotation2334605
version: 1
top level: BBa_K1122006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334605/range2334605/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334605/range2334605/1
type: Range
persistent identity: range2334605
display id: range2334605
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 6
end: 11
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334607
display id: annotation2334607
version: 1
top level: BBa_K1122006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334607/range2334607/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334607/range2334607/1
type: Range
persistent identity: range2334607
display id: range2334607
version: 1
direction: direction/reverse
top level: BBa_K1122006
start: 725
end: 730
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0083
display id: BBa_R0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
mutable notes: The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown. The a at base 41 is mutated to t to avoid XbaI site. Speculation that this was a previous assembly site.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: linker
sequence annotation: C1 OmpR
sequence annotation: -35
sequence annotation: -10
sequence annotation: A
sequence: https://synbiohub.org/public/igem/BBa_R0083_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation301223
display id: annotation301223
version: 1
top level: OmpR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
type: Range
persistent identity: range301223
display id: range301223
version: 1
direction: direction/forward
top level: OmpR
start: 37
end: 44
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301221
display id: annotation301221
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
type: Range
persistent identity: range301221
display id: range301221
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301224
display id: annotation301224
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
type: Range
persistent identity: range301224
display id: range301224
version: 1
direction: direction/forward
top level: OmpR
start: 45
end: 50
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301226
display id: annotation301226
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
type: Range
persistent identity: range301226
display id: range301226
version: 1
direction: direction/forward
top level: OmpR
start: 68
end: 73
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation306881
display id: annotation306881
version: 1
top level: OmpR
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1

https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
type: Range
persistent identity: range306881
display id: range306881
version: 1
direction: direction/forward
top level: OmpR
start: 41
end: 41
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0036
display id: BBa_E0036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0036_sequence/1

BBa_K1033225
type: ComponentDefinition
persistent identity: BBa K1033225
display id: BBa_K1033225
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter CP44
creator: Stephanie Herman, Alona Nyberg
created: 2013-08-22T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 17260
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for a promoter that works in both E. coli and L. bacillus. It is characterized agains J23101, when J23101 had 4322 AU, this promoter had 17518 AU.
mutable notes: This part is designed with the same sticky ends as you would get by cutting it with EcoRI-HF and Spel.
mutable provenance: This part is a synthetic promoter based on a promoter library from the article "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." by Peter Ruhdal Jensen and Karin Hammer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1033225_sequence/1

BBa_K1027001
type: ComponentDefinition
persistent identity: BBa K1027001
display id: BBa_K1027001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Delta 9 Desaturase, optimised for expression in Escherichia coli
creator: Robert Harrison, Lorna Hepworth, Vun Hiang Tan
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:08:46Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1334_
m user id: 0
owner id: 15689
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This enzyme is obtained fro the gene Stearoly Co-A Desaturase (SCD). The gene is obtained from Synechocystis sp., a cyanobacterium.It is involved in the biosynthesize of C18:1 from C18:0. C18:1 corresponds to Oleic acid. The delta 9 desaturase introduces cis carbon double bond on the 9th carbon in C18:0.
mutable notes: Codon optimized for expression in E. coli
mutable provenance: Synthesized gene based off the protein sequence from Synechocystis sp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K1027001_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2357078
display id: annotation2357078
version: 1
top level: BBa_K1027001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1027001/annotation2357078/range2357078/1

https://synbiohub.org/public/igem/BBa_K1027001/annotation2357078/range2357078/1
type: Range
persistent identity: range2357078
display id: range2357078
version: 1
direction: direction/forward
top level: BBa_K1027001
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2357079
display id: annotation2357079
version: 1
top level: BBa_K1027001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1027001/annotation2357079/range2357079/1

https://synbiohub.org/public/igem/BBa_K1027001/annotation2357079/range2357079/1
type: Range
persistent identity: range2357079
display id: range2357079
version: 1
direction: direction/forward
top level: BBa_K1027001
start: 955
end: 960
orientation: inline

BBa_K1159001
type: ComponentDefinition
persistent identity: BBa K1159001
display id: BBa_K1159001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NanoLuc Luciferase in RFC[25]
creator: TU Munich 2013
created: 2013-05-16T11:00:00Z
modified: 2016-01-26T02:12:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NanoLuc Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K1159001_sequence/1

NanoLuc Luciferase
type: SequenceAnnotation
persistent identity: annotation2342942
display id: annotation2342942
version: 1
top level: BBa_K1159001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159001/annotation2342942/range2342942/1

https://synbiohub.org/public/igem/BBa_K1159001/annotation2342942/range2342942/1
type: Range
persistent identity: range2342942
display id: range2342942
version: 1
direction: direction/forward
top level: BBa_K1159001
start: 1
end: 510
orientation: inline

BBa_I13504
type: ComponentDefinition
persistent identity: BBa I13504
display id: BBa_I13504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13504_sequence/1

BBa_B0012
type: Component
persistent identity: component1505084
display id: component1505084
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1505074
display id: component1505074
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1505067
display id: component1505067
version: 1
top level: BBa_I13504
access: public
definition: BBa_B0034

BBa_E0040
type: Component
persistent identity: component1505069
display id: component1505069
version: 1
top level: BBa_I13504
access: public
definition: GFP

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505067
display id: annotation1505067
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
type: Range
persistent identity: range1505067
display id: range1505067
version: 1
direction: direction/forward
top level: BBa_I13504
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505074
display id: annotation1505074
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
type: Range
persistent identity: range1505074
display id: range1505074
version: 1
direction: direction/forward
top level: BBa_I13504
start: 747
end: 826
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505069
display id: annotation1505069
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
type: Range
persistent identity: range1505069
display id: range1505069
version: 1
direction: direction/forward
top level: BBa_I13504
start: 19
end: 738
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505084
display id: annotation1505084
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
type: Range
persistent identity: range1505084
display id: range1505084
version: 1
direction: direction/forward
top level: BBa_I13504
start: 835
end: 875
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

CmR
type: ComponentDefinition
persistent identity: BBa J61000
display id: BBa_J61000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol resistance cassette
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol. {pSB1A2-Bca1016}
mutable notes: N/A
mutable provenance: PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Products are Bca1015 and Bca1016 ---- ca1016F Forward EcoRI to biobrick CAT of pKD3 gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg ca1016R Reverse SpeI to biobrick CAT of pKD3 ccgctactagtggcgcgcctacctgtgacgg
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_J61000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1892270
display id: annotation1892270
version: 1
top level: CmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1

https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
type: Range
persistent identity: range1892270
display id: range1892270
version: 1
direction: direction/reverse
top level: CmR
start: 30
end: 687
orientation: inline

BBa_I13401
type: ComponentDefinition
persistent identity: BBa I13401
display id: BBa_I13401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP reporter for RHS of library test constructs
creator: jasonk
created: 2005-04-05T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part will be suffixed to a promoter.RBS library. The purpose of the experiment is to make a first pass at a library-based construction step to identify efficiency issues and also to examine maxPOPS/RIPS level.
mutable notes: This part was built by Jen as an intermediate to their nuts and bolts parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13401_sequence/1

BBa_B0010
type: Component
persistent identity: component1476718
display id: component1476718
version: 1
top level: BBa_I13401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1476713
display id: component1476713
version: 1
top level: BBa_I13401
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component1476728
display id: component1476728
version: 1
top level: BBa_I13401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1476713
display id: annotation1476713
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
type: Range
persistent identity: range1476713
display id: range1476713
version: 1
direction: direction/forward
top level: BBa_I13401
start: 1
end: 720
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1476718
display id: annotation1476718
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
type: Range
persistent identity: range1476718
display id: range1476718
version: 1
direction: direction/forward
top level: BBa_I13401
start: 729
end: 808
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1476728
display id: annotation1476728
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
type: Range
persistent identity: range1476728
display id: range1476728
version: 1
direction: direction/forward
top level: BBa_I13401
start: 817
end: 857
orientation: inline

BBa_K1114003
type: ComponentDefinition
persistent identity: BBa K1114003
display id: BBa_K1114003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23103 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114003_sequence/1

BBa_K1982006
type: ComponentDefinition
persistent identity: BBa K1982006
display id: BBa_K1982006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tCas9-Vp64(Prokaryotic)
creator: Zexu Li
created: 2016-09-11T11:00:00Z
modified: 2016-10-18T10:24:08Z
discontinued: false
dominant: false
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The CRY2/CIBN interaction is entirely genetically encoded. The binding reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark. This fusion protein is for use in LACE(light-activated CRISPR/Cas9 effector) system, and a tCas9 fused to its N terminus. To regulate DNA transcription by blue light, the system is based on CRY2/CIBN interaction in which a light-mediated protein interaction brings together two protein (tCas9 and an activation domain VP64) . If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIBN and shut off transcription. tCas9 can be tagged with transcriptional activators, and targeting these dCas9 fusion proteins to the promoter region results in robust transcription activation of downstream target genes. This tCas9-based activators is the case that tCas9 fused directly to a single transcriptional activator( VP64).
mutable notes: mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg
mutable provenance: tCas9 is from GE Share company. VP64 is synthetic construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: VP16
sequence annotation: Prokaryotic rbs
sequence annotation: SV40 NLS
sequence annotation: HA
sequence annotation: tCas9
sequence annotation: Linker
sequence annotation: FLAG
sequence annotation: ATG
sequence annotation: TAATAA
sequence: https://synbiohub.org/public/igem/BBa_K1982006_sequence/1

VP16
type: SequenceAnnotation
persistent identity: annotation2517248
display id: annotation2517248
version: 1
top level: BBa_K1982006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517248/range2517248/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517248/range2517248/1
type: Range
persistent identity: range2517248
display id: range2517248
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4261
end: 4299
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517247
display id: annotation2517247
version: 1
top level: BBa_K1982006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517247/range2517247/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517247/range2517247/1
type: Range
persistent identity: range2517247
display id: range2517247
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4222
end: 4260
orientation: inline

Prokaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2517241
display id: annotation2517241
version: 1
top level: BBa_K1982006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517241/range2517241/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517241/range2517241/1
type: Range
persistent identity: range2517241
display id: range2517241
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 1
end: 18
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2517244
display id: annotation2517244
version: 1
top level: BBa_K1982006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517244/range2517244/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517244/range2517244/1
type: Range
persistent identity: range2517244
display id: range2517244
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4123
end: 4143
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation2517250
display id: annotation2517250
version: 1
top level: BBa_K1982006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517250/range2517250/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517250/range2517250/1
type: Range
persistent identity: range2517250
display id: range2517250
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4306
end: 4332
orientation: inline

tCas9
type: SequenceAnnotation
persistent identity: annotation2517243
display id: annotation2517243
version: 1
top level: BBa_K1982006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517243/range2517243/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517243/range2517243/1
type: Range
persistent identity: range2517243
display id: range2517243
version: 1
direction: direction/reverse
top level: BBa_K1982006
start: 22
end: 4122
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517245
display id: annotation2517245
version: 1
top level: BBa_K1982006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517245/range2517245/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517245/range2517245/1
type: Range
persistent identity: range2517245
display id: range2517245
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4144
end: 4182
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517246
display id: annotation2517246
version: 1
top level: BBa_K1982006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517246/range2517246/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517246/range2517246/1
type: Range
persistent identity: range2517246
display id: range2517246
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4183
end: 4221
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2517249
display id: annotation2517249
version: 1
top level: BBa_K1982006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517249/range2517249/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517249/range2517249/1
type: Range
persistent identity: range2517249
display id: range2517249
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4300
end: 4305
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2517251
display id: annotation2517251
version: 1
top level: BBa_K1982006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517251/range2517251/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517251/range2517251/1
type: Range
persistent identity: range2517251
display id: range2517251
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4339
end: 4362
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2517242
display id: annotation2517242
version: 1
top level: BBa_K1982006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517242/range2517242/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517242/range2517242/1
type: Range
persistent identity: range2517242
display id: range2517242
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 19
end: 21
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2517252
display id: annotation2517252
version: 1
top level: BBa_K1982006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982006/annotation2517252/range2517252/1

https://synbiohub.org/public/igem/BBa_K1982006/annotation2517252/range2517252/1
type: Range
persistent identity: range2517252
display id: range2517252
version: 1
direction: direction/forward
top level: BBa_K1982006
start: 4363
end: 4368
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.4 (weaker) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0033
sequence annotation: RBS-4\Weaker
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
direction: direction/forward
top level: BBa_B0033
start: 7
end: 10
orientation: inline

BBa_K1104301
type: ComponentDefinition
persistent identity: BBa K1104301
display id: BBa_K1104301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Defensin1
creator: Jou-Chien Liao, Rohan Shina, Yucheng Huang
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1415_
m user id: 0
owner id: 16321
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Defensin1
mutable notes: None
mutable provenance: ''Apis melliferra''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mature peptide
sequence annotation: Methionine
sequence annotation: Term
sequence: https://synbiohub.org/public/igem/BBa_K1104301_sequence/1

mature peptide
type: SequenceAnnotation
persistent identity: annotation2363644
display id: annotation2363644
version: 1
top level: BBa_K1104301
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1104301/annotation2363644/range2363644/1

https://synbiohub.org/public/igem/BBa_K1104301/annotation2363644/range2363644/1
type: Range
persistent identity: range2363644
display id: range2363644
version: 1
direction: direction/forward
top level: BBa_K1104301
start: 4
end: 159
orientation: inline

Methionine
type: SequenceAnnotation
persistent identity: annotation2363581
display id: annotation2363581
version: 1
top level: BBa_K1104301
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1104301/annotation2363581/range2363581/1

https://synbiohub.org/public/igem/BBa_K1104301/annotation2363581/range2363581/1
type: Range
persistent identity: range2363581
display id: range2363581
version: 1
direction: direction/forward
top level: BBa_K1104301
start: 1
end: 3
orientation: inline

Term
type: SequenceAnnotation
persistent identity: annotation2363582
display id: annotation2363582
version: 1
top level: BBa_K1104301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1104301/annotation2363582/range2363582/1

https://synbiohub.org/public/igem/BBa_K1104301/annotation2363582/range2363582/1
type: Range
persistent identity: range2363582
display id: range2363582
version: 1
direction: direction/forward
top level: BBa_K1104301
start: 160
end: 162
orientation: inline

BBa_K1319004
type: ComponentDefinition
persistent identity: BBa K1319004
display id: BBa_K1319004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli
creator: Florian Gohr, Michael Osthege
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.
mutable notes: The part does not contain start or stop codons, because it was designed for RFC25 compability
mutable provenance: The part was codon optimized using the genomic amino acid sequence, designed for RFC25 and mutated S219V for anti-self cleavage properties.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: S219V
sequence annotation: TEV protease
sequence: https://synbiohub.org/public/igem/BBa_K1319004_sequence/1

S219V
type: SequenceAnnotation
persistent identity: annotation2386540
display id: annotation2386540
version: 1
top level: BBa_K1319004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1319004/annotation2386540/range2386540/1

https://synbiohub.org/public/igem/BBa_K1319004/annotation2386540/range2386540/1
type: Range
persistent identity: range2386540
display id: range2386540
version: 1
direction: direction/forward
top level: BBa_K1319004
start: 655
end: 657
orientation: inline

TEV protease
type: SequenceAnnotation
persistent identity: annotation2386539
display id: annotation2386539
version: 1
top level: BBa_K1319004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1319004/annotation2386539/range2386539/1

https://synbiohub.org/public/igem/BBa_K1319004/annotation2386539/range2386539/1
type: Range
persistent identity: range2386539
display id: range2386539
version: 1
direction: direction/forward
top level: BBa_K1319004
start: 1
end: 726
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15017
display id: BBa_I15017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.EYFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to generate EYFP under the medium strength RBS B0032.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0030
component: BBa_B0032
sequence annotation: BBa_E0030
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I15017_sequence/1

BBa_E0030
type: Component
persistent identity: component1478422
display id: component1478422
version: 1
top level: ecfp
access: public
definition: eyfp

BBa_B0032
type: Component
persistent identity: component1478419
display id: component1478419
version: 1
top level: ecfp
access: public
definition: BBa_B0032

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1478422
display id: annotation1478422
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
type: Range
persistent identity: range1478422
display id: range1478422
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478419
display id: annotation1478419
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
type: Range
persistent identity: range1478419
display id: range1478419
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

BBa_K797004
type: ComponentDefinition
persistent identity: BBa K797004
display id: BBa_K797004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tat secretion cassette with constitutive promoter.
creator: Tomohiro Nobeyama
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
group u list: _1053_
m user id: 0
owner id: 11588
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This cassette allow E.coli to secrete proteins with torA signal. Wild type Tat protein secretion system is too week so that Kyoto 2012 construct Tat cassette to reinforce the ability of transportation of Tat system. This parts include tatA,B,C protein coding region and pspA (phage shock protein A). Tat A,B,C protein is the main component of Tat complex where proteins with torA signal go through and pspA can encourage protein secretion via Tat system. Kyoto 2012 suggest iGEMers with this new way of secretion and provide them with this cassette regulated by constitutive promoter.
mutable notes: This Device has too long sequence to read it so that we read each components' sequence separately and conjugated them to make the information about sequence of the device
mutable provenance: tatABCD and pspA are derived from Escherichia coli's genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: tatABCD
sequence annotation: pspA
sequence annotation: J23107
sequence: https://synbiohub.org/public/igem/BBa_K797004_sequence/1

tatABCD
type: SequenceAnnotation
persistent identity: annotation2195217
display id: annotation2195217
version: 1
top level: BBa_K797004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797004/annotation2195217/range2195217/1

https://synbiohub.org/public/igem/BBa_K797004/annotation2195217/range2195217/1
type: Range
persistent identity: range2195217
display id: range2195217
version: 1
direction: direction/forward
top level: BBa_K797004
start: 163
end: 2554
orientation: inline

pspA
type: SequenceAnnotation
persistent identity: annotation2195220
display id: annotation2195220
version: 1
top level: BBa_K797004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K797004/annotation2195220/range2195220/1

https://synbiohub.org/public/igem/BBa_K797004/annotation2195220/range2195220/1
type: Range
persistent identity: range2195220
display id: range2195220
version: 1
direction: direction/forward
top level: BBa_K797004
start: 2718
end: 3386
orientation: inline

J23107
type: SequenceAnnotation
persistent identity: annotation2195216
display id: annotation2195216
version: 1
top level: BBa_K797004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K797004/annotation2195216/range2195216/1

https://synbiohub.org/public/igem/BBa_K797004/annotation2195216/range2195216/1
type: Range
persistent identity: range2195216
display id: range2195216
version: 1
direction: direction/forward
top level: BBa_K797004
start: 1
end: 35
orientation: inline

BBa_K1033929
type: ComponentDefinition
persistent identity: BBa K1033929
display id: BBa_K1033929
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue, blue chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.
mutable notes: Synthesized and codon optimized for E. coli by Bioneer Corp.
mutable provenance: Actinia equina
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K864401
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K864401
sequence: https://synbiohub.org/public/igem/BBa_K1033929_sequence/1

BBa_K864401
type: Component
persistent identity: component2351877
display id: component2351877
version: 1
top level: BBa_K1033929
access: public
definition: https://synbiohub.org/public/igem/BBa_K864401/1

BBa_B0034
type: Component
persistent identity: component2351875
display id: component2351875
version: 1
top level: BBa_K1033929
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351875
display id: annotation2351875
version: 1
top level: BBa_K1033929
location: https://synbiohub.org/public/igem/BBa_K1033929/annotation2351875/range2351875/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033929/annotation2351875/range2351875/1
type: Range
persistent identity: range2351875
display id: range2351875
version: 1
direction: direction/reverse
top level: BBa_K1033929
start: 1
end: 12
orientation: inline

BBa_K864401
type: SequenceAnnotation
persistent identity: annotation2351877
display id: annotation2351877
version: 1
top level: BBa_K1033929
location: https://synbiohub.org/public/igem/BBa_K1033929/annotation2351877/range2351877/1
component: BBa_K864401

https://synbiohub.org/public/igem/BBa_K1033929/annotation2351877/range2351877/1
type: Range
persistent identity: range2351877
display id: range2351877
version: 1
direction: direction/reverse
top level: BBa_K1033929
start: 19
end: 717
orientation: inline

BBa_I13500
type: ComponentDefinition
persistent identity: BBa I13500
display id: BBa_I13500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13500_sequence/1

BBa_E0040
type: Component
persistent identity: component1505002
display id: component1505002
version: 1
top level: BBa_I13500
access: public
definition: GFP

BBa_B0034
type: Component
persistent identity: component1505000
display id: component1505000
version: 1
top level: BBa_I13500
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505000
display id: annotation1505000
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
type: Range
persistent identity: range1505000
display id: range1505000
version: 1
direction: direction/forward
top level: BBa_I13500
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505002
display id: annotation1505002
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
type: Range
persistent identity: range1505002
display id: range1505002
version: 1
direction: direction/forward
top level: BBa_I13500
start: 19
end: 738
orientation: inline

BBa_K783031
type: ComponentDefinition
persistent identity: BBa K783031
display id: BBa_K783031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_J23114
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_J23114. It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_J23114
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K783031_sequence/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2214229
display id: annotation2214229
version: 1
top level: BBa_K783031
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K783031/annotation2214229/range2214229/1

https://synbiohub.org/public/igem/BBa_K783031/annotation2214229/range2214229/1
type: Range
persistent identity: range2214229
display id: range2214229
version: 1
direction: direction/reverse
top level: BBa_K783031
start: 1
end: 35
orientation: inline

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

BBa_K1114108
type: ComponentDefinition
persistent identity: BBa K1114108
display id: BBa_K1114108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic design RBS 8, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BCD8
sequence annotation: MoClo Fusion Site B
sequence annotation: MoClo Fusion Site C
sequence: https://synbiohub.org/public/igem/BBa_K1114108_sequence/1

BCD8
type: SequenceAnnotation
persistent identity: annotation2361723
display id: annotation2361723
version: 1
top level: BBa_K1114108
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114108/annotation2361723/range2361723/1

https://synbiohub.org/public/igem/BBa_K1114108/annotation2361723/range2361723/1
type: Range
persistent identity: range2361723
display id: range2361723
version: 1
direction: direction/forward
top level: BBa_K1114108
start: 5
end: 88
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361725
display id: annotation2361725
version: 1
top level: BBa_K1114108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114108/annotation2361725/range2361725/1

https://synbiohub.org/public/igem/BBa_K1114108/annotation2361725/range2361725/1
type: Range
persistent identity: range2361725
display id: range2361725
version: 1
direction: direction/forward
top level: BBa_K1114108
start: 1
end: 4
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361726
display id: annotation2361726
version: 1
top level: BBa_K1114108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114108/annotation2361726/range2361726/1

https://synbiohub.org/public/igem/BBa_K1114108/annotation2361726/range2361726/1
type: Range
persistent identity: range2361726
display id: range2361726
version: 1
direction: direction/forward
top level: BBa_K1114108
start: 89
end: 92
orientation: inline

BBa_K1033907
type: ComponentDefinition
persistent identity: BBa K1033907
display id: BBa_K1033907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from FezziwigYFP vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033909
component: BBa_J23110
sequence annotation: BBa_K1033909
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K1033907_sequence/1

BBa_K1033909
type: Component
persistent identity: component2371228
display id: component2371228
version: 1
top level: BBa_K1033907
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033909/1

BBa_J23110
type: Component
persistent identity: component2371224
display id: component2371224
version: 1
top level: BBa_K1033907
access: public
definition: BBa_J23110

BBa_K1033909
type: SequenceAnnotation
persistent identity: annotation2371228
display id: annotation2371228
version: 1
top level: BBa_K1033907
location: https://synbiohub.org/public/igem/BBa_K1033907/annotation2371228/range2371228/1
component: BBa_K1033909

https://synbiohub.org/public/igem/BBa_K1033907/annotation2371228/range2371228/1
type: Range
persistent identity: range2371228
display id: range2371228
version: 1
direction: direction/reverse
top level: BBa_K1033907
start: 44
end: 775
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2371224
display id: annotation2371224
version: 1
top level: BBa_K1033907
location: https://synbiohub.org/public/igem/BBa_K1033907/annotation2371224/range2371224/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033907/annotation2371224/range2371224/1
type: Range
persistent identity: range2371224
display id: range2371224
version: 1
direction: direction/reverse
top level: BBa_K1033907
start: 1
end: 35
orientation: inline

BBa_K1033926
type: ComponentDefinition
persistent identity: BBa K1033926
display id: BBa_K1033926
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asPink, pink chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia sulcata, asCP, naturally exhibits color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia sulcata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23110
component: BBa_K1033927
sequence annotation: BBa_J23110
sequence annotation: BBa_K1033927
sequence: https://synbiohub.org/public/igem/BBa_K1033926_sequence/1

BBa_J23110
type: Component
persistent identity: component2351865
display id: component2351865
version: 1
top level: BBa_K1033926
access: public
definition: BBa_J23110

BBa_K1033927
type: Component
persistent identity: component2351869
display id: component2351869
version: 1
top level: BBa_K1033926
access: public
definition: asPink

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2351865
display id: annotation2351865
version: 1
top level: BBa_K1033926
location: https://synbiohub.org/public/igem/BBa_K1033926/annotation2351865/range2351865/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033926/annotation2351865/range2351865/1
type: Range
persistent identity: range2351865
display id: range2351865
version: 1
direction: direction/reverse
top level: BBa_K1033926
start: 1
end: 35
orientation: inline

BBa_K1033927
type: SequenceAnnotation
persistent identity: annotation2351869
display id: annotation2351869
version: 1
top level: BBa_K1033926
location: https://synbiohub.org/public/igem/BBa_K1033926/annotation2351869/range2351869/1
component: BBa_K1033927

https://synbiohub.org/public/igem/BBa_K1033926/annotation2351869/range2351869/1
type: Range
persistent identity: range2351869
display id: range2351869
version: 1
direction: direction/reverse
top level: BBa_K1033926
start: 44
end: 763
orientation: inline

BBa_K1150000
type: ComponentDefinition
persistent identity: BBa K1150000
display id: BBa_K1150000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9
creator: Freiburg 2013
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The human codon optimized Cas9 from the organism Streptococcus pyogenes was standardized (RFC 25). Additionally we mutated the Nickase function. In the end we engineered a DNA binding protein, that can be fused with different effectors in order to regulate gene expression.
mutable notes: Restriction sites were deleted by primer overhangs. Parts were fused together by fusion PCR. The nickase function was mutated in order to abbolish the demolition of DNA.
mutable provenance: Streptococcus pyogenes, Zhang lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Nickaseless mutation
sequence annotation: dCas9
sequence: https://synbiohub.org/public/igem/BBa_K1150000_sequence/1

Nickaseless mutation
type: SequenceAnnotation
persistent identity: annotation2354601
display id: annotation2354601
version: 1
top level: BBa_K1150000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1150000/annotation2354601/range2354601/1

https://synbiohub.org/public/igem/BBa_K1150000/annotation2354601/range2354601/1
type: Range
persistent identity: range2354601
display id: range2354601
version: 1
direction: direction/forward
top level: BBa_K1150000
start: 2515
end: 2517
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2347618
display id: annotation2347618
version: 1
top level: BBa_K1150000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150000/annotation2347618/range2347618/1

https://synbiohub.org/public/igem/BBa_K1150000/annotation2347618/range2347618/1
type: Range
persistent identity: range2347618
display id: range2347618
version: 1
direction: direction/forward
top level: BBa_K1150000
start: 1
end: 4101
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR Protein Generator
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0034
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
access: public
definition: BBa_B0034

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
access: public
definition: luxr

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
direction: direction/forward
top level: LuxR
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
direction: direction/forward
top level: LuxR
start: 896
end: 936
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
direction: direction/forward
top level: LuxR
start: 19
end: 774
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
direction: direction/forward
top level: LuxR
start: 1
end: 12
orientation: inline

sfCFP
type: ComponentDefinition
persistent identity: BBa K1093002
display id: BBa_K1093002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfCFP
creator: Anna Miścicka
created: 2013-09-19T11:00:00Z
modified: 2016-01-26T03:05:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1403_
m user id: 4206
owner id: 18431
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Superfolder Cyan Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to CFP it displays improved stability and strong cyan fluorescence.
mutable notes: Derivative of sfGFP, made by PCR
mutable provenance: PartsRegistry BBa_I746909, originally come from Aequorea victoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sfCFP
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1093002_sequence/1

sfCFP
type: SequenceAnnotation
persistent identity: annotation2367769
display id: annotation2367769
version: 1
top level: sfCFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1093002/annotation2367769/range2367769/1

https://synbiohub.org/public/igem/BBa_K1093002/annotation2367769/range2367769/1
type: Range
persistent identity: range2367769
display id: range2367769
version: 1
direction: direction/forward
top level: sfCFP
start: 1
end: 715
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2367767
display id: annotation2367767
version: 1
top level: sfCFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1093002/annotation2367767/range2367767/1

https://synbiohub.org/public/igem/BBa_K1093002/annotation2367767/range2367767/1
type: Range
persistent identity: range2367767
display id: range2367767
version: 1
direction: direction/forward
top level: sfCFP
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2367768
display id: annotation2367768
version: 1
top level: sfCFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1093002/annotation2367768/range2367768/1

https://synbiohub.org/public/igem/BBa_K1093002/annotation2367768/range2367768/1
type: Range
persistent identity: range2367768
display id: range2367768
version: 1
direction: direction/forward
top level: sfCFP
start: 715
end: 720
orientation: inline

BBa_K1467102
type: ComponentDefinition
persistent identity: BBa K1467102
display id: BBa_K1467102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible BS3 promoter + 5
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:36:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: BS3 is a plant specific promoter which is induced by the TALE AvrBS3. This part is compatible with the GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences).
mutable notes: Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences).
mutable provenance: Capsicum annum
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467102_sequence/1

BBa_K115001
type: ComponentDefinition
persistent identity: BBa K115001
display id: BBa_K115001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (ROSE)
creator: O.M.J.A. Stassen
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3007
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is designed as a temperature inducible RBS. Only designed so far.
mutable notes: The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
mutable provenance: This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein (prfA).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Predicted Stem Loop extending to startcodon
sequence annotation: Predicted Stem Loop
sequence annotation: Biobrick-Scar-adaptation
sequence annotation: SD
sequence: https://synbiohub.org/public/igem/BBa_K115001_sequence/1

Predicted Stem Loop extending to startcodon
type: SequenceAnnotation
persistent identity: annotation1966929
display id: annotation1966929
version: 1
top level: BBa_K115001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966929/range1966929/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966929/range1966929/1
type: Range
persistent identity: range1966929
display id: range1966929
version: 1
direction: direction/forward
top level: BBa_K115001
start: 72
end: 96
orientation: inline

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966927
display id: annotation1966927
version: 1
top level: BBa_K115001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966927/range1966927/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966927/range1966927/1
type: Range
persistent identity: range1966927
display id: range1966927
version: 1
direction: direction/forward
top level: BBa_K115001
start: 16
end: 36
orientation: inline

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966928
display id: annotation1966928
version: 1
top level: BBa_K115001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966928/range1966928/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966928/range1966928/1
type: Range
persistent identity: range1966928
display id: range1966928
version: 1
direction: direction/forward
top level: BBa_K115001
start: 39
end: 71
orientation: inline

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966926
display id: annotation1966926
version: 1
top level: BBa_K115001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966926/range1966926/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966926/range1966926/1
type: Range
persistent identity: range1966926
display id: range1966926
version: 1
direction: direction/forward
top level: BBa_K115001
start: 1
end: 15
orientation: inline

Biobrick-Scar-adaptation
type: SequenceAnnotation
persistent identity: annotation1966898
display id: annotation1966898
version: 1
top level: BBa_K115001
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966898/range1966898/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966898/range1966898/1
type: Range
persistent identity: range1966898
display id: range1966898
version: 1
direction: direction/forward
top level: BBa_K115001
start: 74
end: 79
orientation: inline

SD
type: SequenceAnnotation
persistent identity: annotation1966896
display id: annotation1966896
version: 1
top level: BBa_K115001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966896/range1966896/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966896/range1966896/1
type: Range
persistent identity: range1966896
display id: range1966896
version: 1
direction: direction/forward
top level: BBa_K115001
start: 90
end: 95
orientation: inline

Val-Ind
type: ComponentDefinition
persistent identity: BBa K1152006
display id: BBa_K1152006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Valine-Indigoidine fusion - NRPS
creator: Philipp D. Walch
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:09:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1464_
m user id: 0
owner id: 16422
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: X
mutable notes: X
mutable provenance: X
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TycC4dC
sequence annotation: indC
sequence annotation: -35
sequence annotation: -10
sequence annotation: C(TycC2)
sequence annotation: stop
sequence annotation: start
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_K1152006_sequence/1

TycC4dC
type: SequenceAnnotation
persistent identity: annotation2368763
display id: annotation2368763
version: 1
top level: Val-Ind
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1152006/annotation2368763/range2368763/1

https://synbiohub.org/public/igem/BBa_K1152006/annotation2368763/range2368763/1
type: Range
persistent identity: range2368763
display id: range2368763
version: 1
direction: direction/forward
top level: Val-Ind
start: 228
end: 1958
orientation: inline

indC
type: SequenceAnnotation
persistent identity: annotation2368779
display id: annotation2368779
version: 1
top level: Val-Ind
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1152006/annotation2368779/range2368779/1

https://synbiohub.org/public/igem/BBa_K1152006/annotation2368779/range2368779/1
type: Range
persistent identity: range2368779
display id: range2368779
version: 1
direction: direction/forward
top level: Val-Ind
start: 2880
end: 6734
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2368730
display id: annotation2368730
version: 1
top level: Val-Ind
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1152006/annotation2368730/range2368730/1

https://synbiohub.org/public/igem/BBa_K1152006/annotation2368730/range2368730/1
type: Range
persistent identity: range2368730
display id: range2368730
version: 1
direction: direction/forward
top level: Val-Ind
start: 138
end: 143
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2368761
display id: annotation2368761
version: 1
top level: Val-Ind
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1152006/annotation2368761/range2368761/1

https://synbiohub.org/public/igem/BBa_K1152006/annotation2368761/range2368761/1
type: Range
persistent identity: range2368761
display id: range2368761
version: 1
direction: direction/forward
top level: Val-Ind
start: 162
end: 167
orientation: inline

C(TycC2)
type: SequenceAnnotation
persistent identity: annotation2368764
display id: annotation2368764
version: 1
top level: Val-Ind
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1152006/annotation2368764/range2368764/1

https://synbiohub.org/public/igem/BBa_K1152006/annotation2368764/range2368764/1
type: Range
persistent identity: range2368764
display id: range2368764
version: 1
direction: direction/forward
top level: Val-Ind
start: 1959
end: 2879
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2368791
display id: annotation2368791
version: 1
top level: Val-Ind
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1152006/annotation2368791/range2368791/1

https://synbiohub.org/public/igem/BBa_K1152006/annotation2368791/range2368791/1
type: Range
persistent identity: range2368791
display id: range2368791
version: 1
direction: direction/forward
top level: Val-Ind
start: 6895
end: 6895
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2368762
display id: annotation2368762
version: 1
top level: Val-Ind
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1152006/annotation2368762/range2368762/1

https://synbiohub.org/public/igem/BBa_K1152006/annotation2368762/range2368762/1
type: Range
persistent identity: range2368762
display id: range2368762
version: 1
direction: direction/forward
top level: Val-Ind
start: 174
end: 174
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2368790
display id: annotation2368790
version: 1
top level: Val-Ind
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1152006/annotation2368790/range2368790/1

https://synbiohub.org/public/igem/BBa_K1152006/annotation2368790/range2368790/1
type: Range
persistent identity: range2368790
display id: range2368790
version: 1
direction: direction/forward
top level: Val-Ind
start: 6869
end: 6888
orientation: inline

gldA
type: ComponentDefinition
persistent identity: BBa K1172201
display id: BBa_K1172201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GldA - glycerol dehydrogenase from Escherichia coli
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Gene gldA encodes glycerol dehydrogenase from Escherichicha coli. The overexpression of glycerol dehydrogenase in E. coli can be used as a way for endogenous mediator production. Many derivates of glycerol dehydrogenase are small, water-soluble redoxmolecules, which have mediator properties. In enzymology, a glycerol dehydrogenase (EC 1.1.1.6) is an enzyme belonging to the family of oxireductases that catalyzes the chemical reaction: Glycerol + NAD --> Glycerone + NADH + H
mutable notes: Primeroverlap for Gibson-Assembly
mutable provenance: Escherichicha coli KRX genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: gldA
sequence: https://synbiohub.org/public/igem/BBa_K1172201_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2370298
display id: annotation2370298
version: 1
top level: gldA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2370298/range2370298/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2370298/range2370298/1
type: Range
persistent identity: range2370298
display id: range2370298
version: 1
direction: direction/forward
top level: gldA
start: 1102
end: 1104
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370297
display id: annotation2370297
version: 1
top level: gldA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2370297/range2370297/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2370297/range2370297/1
type: Range
persistent identity: range2370297
display id: range2370297
version: 1
direction: direction/forward
top level: gldA
start: 1
end: 3
orientation: inline

gldA
type: SequenceAnnotation
persistent identity: annotation2332911
display id: annotation2332911
version: 1
top level: gldA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2332911/range2332911/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2332911/range2332911/1
type: Range
persistent identity: range2332911
display id: range2332911
version: 1
direction: direction/forward
top level: gldA
start: 1
end: 1104
orientation: inline

BBa_K1114010
type: ComponentDefinition
persistent identity: BBa K1114010
display id: BBa_K1114010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23107 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23107 with AB fusion sites. See the BBa_J23107 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable notes: This is the MoClo formatted part of BBa_J23107 with AB fusion sites. See the BBa_J23107 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114010_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa I13502
display id: BBa_I13502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I13502_sequence/1

BBa_B0034
type: Component
persistent identity: component1505038
display id: component1505038
version: 1
top level: mRFP1
access: public
definition: BBa_B0034

BBa_E1010
type: Component
persistent identity: component1505045
display id: component1505045
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505038
display id: annotation1505038
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
type: Range
persistent identity: range1505038
display id: range1505038
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505045
display id: annotation1505045
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
type: Range
persistent identity: range1505045
display id: range1505045
version: 1
direction: direction/forward
top level: mRFP1
start: 19
end: 699
orientation: inline

BBa_K1073026
type: ComponentDefinition
persistent identity: BBa K1073026
display id: BBa_K1073026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutively expressed chromoprotein cjBlue
creator: iGEM Team Braunschweig 2013
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The chromoprotein cjblue is constitutively expressed and can be used for the marking of cells for easier differentiation.
mutable notes: no considerations.
mutable provenance: This part is a combination of BBa_K1073006 and BBa_K592011, which were derived from 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1073006
component: BBa_K592011
sequence annotation: BBa_K592011
sequence annotation: BBa_K1073006
sequence: https://synbiohub.org/public/igem/BBa_K1073026_sequence/1

BBa_K1073006
type: Component
persistent identity: component2354091
display id: component2354091
version: 1
top level: BBa_K1073026
access: public
definition: https://synbiohub.org/public/igem/BBa_K1073006/1

BBa_K592011
type: Component
persistent identity: component2354093
display id: component2354093
version: 1
top level: BBa_K1073026
access: public
definition: https://synbiohub.org/public/igem/BBa_K592011/1

BBa_K592011
type: SequenceAnnotation
persistent identity: annotation2354093
display id: annotation2354093
version: 1
top level: BBa_K1073026
location: https://synbiohub.org/public/igem/BBa_K1073026/annotation2354093/range2354093/1
component: BBa_K592011

https://synbiohub.org/public/igem/BBa_K1073026/annotation2354093/range2354093/1
type: Range
persistent identity: range2354093
display id: range2354093
version: 1
direction: direction/reverse
top level: BBa_K1073026
start: 63
end: 764
orientation: inline

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354091
display id: annotation2354091
version: 1
top level: BBa_K1073026
location: https://synbiohub.org/public/igem/BBa_K1073026/annotation2354091/range2354091/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073026/annotation2354091/range2354091/1
type: Range
persistent identity: range2354091
display id: range2354091
version: 1
direction: direction/reverse
top level: BBa_K1073026
start: 1
end: 56
orientation: inline

BBa_K1231000
type: ComponentDefinition
persistent identity: BBa K1231000
display id: BBa_K1231000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The asr promoter is a pH-responsive promoter.
creator: Viral Patel
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1545_
m user id: 0
owner id: 18773
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains the asr promoter with its native RBS. The asr promoter is a pH-responsive promoter native to E. coli. It induces transcription in acidic conditions (~pH 5.5).
mutable notes: None.
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1231000_sequence/1

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.2 (weak) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
direction: direction/forward
top level: BBa_B0031
start: 7
end: 10
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: engineered cyan fluorescent protein derived from A. victoria GFP
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_P0440
type: ComponentDefinition
persistent identity: BBa P0440
display id: BBa_P0440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0151]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_P0440_sequence/1

BBa_B0010
type: Component
persistent identity: component944902
display id: component944902
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component944886
display id: component944886
version: 1
top level: BBa_P0440
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component944912
display id: component944912
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component944896
display id: component944896
version: 1
top level: BBa_P0440
access: public
definition: tetR

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944902
display id: annotation944902
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
type: Range
persistent identity: range944902
display id: range944902
version: 1
direction: direction/forward
top level: BBa_P0440
start: 712
end: 791
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944912
display id: annotation944912
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
type: Range
persistent identity: range944912
display id: range944912
version: 1
direction: direction/forward
top level: BBa_P0440
start: 800
end: 840
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944896
display id: annotation944896
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
type: Range
persistent identity: range944896
display id: range944896
version: 1
direction: direction/forward
top level: BBa_P0440
start: 19
end: 678
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944886
display id: annotation944886
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
type: Range
persistent identity: range944886
display id: range944886
version: 1
direction: direction/forward
top level: BBa_P0440
start: 1
end: 12
orientation: inline

BBa_F2622
type: ComponentDefinition
persistent identity: BBa F2622
display id: BBa_F2622
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Receiver Device
creator: Barry Canton
created: 2004-12-14T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the lac repressible R0011.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_R0011
component: BBa_C0062
component: BBa_R0062
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_F2622_sequence/1

BBa_B0010
type: Component
persistent identity: component1484671
display id: component1484671
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1484639
display id: component1484639
version: 1
top level: BBa_F2622
access: public
definition: lacI+pL

BBa_C0062
type: Component
persistent identity: component1484663
display id: component1484663
version: 1
top level: BBa_F2622
access: public
definition: luxr

BBa_R0062
type: Component
persistent identity: component1484696
display id: component1484696
version: 1
top level: BBa_F2622
access: public
definition: lux pR

BBa_B0034
type: Component
persistent identity: component1484648
display id: component1484648
version: 1
top level: BBa_F2622
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component1484681
display id: component1484681
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1484696
display id: annotation1484696
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484696/range1484696/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_F2622/annotation1484696/range1484696/1
type: Range
persistent identity: range1484696
display id: range1484696
version: 1
direction: direction/forward
top level: BBa_F2622
start: 1008
end: 1062
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484671
display id: annotation1484671
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484671/range1484671/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2622/annotation1484671/range1484671/1
type: Range
persistent identity: range1484671
display id: range1484671
version: 1
direction: direction/forward
top level: BBa_F2622
start: 871
end: 950
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484681
display id: annotation1484681
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484681/range1484681/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F2622/annotation1484681/range1484681/1
type: Range
persistent identity: range1484681
display id: range1484681
version: 1
direction: direction/forward
top level: BBa_F2622
start: 959
end: 999
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1484639
display id: annotation1484639
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484639/range1484639/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_F2622/annotation1484639/range1484639/1
type: Range
persistent identity: range1484639
display id: range1484639
version: 1
direction: direction/forward
top level: BBa_F2622
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484648
display id: annotation1484648
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484648/range1484648/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2622/annotation1484648/range1484648/1
type: Range
persistent identity: range1484648
display id: range1484648
version: 1
direction: direction/forward
top level: BBa_F2622
start: 64
end: 75
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1484663
display id: annotation1484663
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484663/range1484663/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2622/annotation1484663/range1484663/1
type: Range
persistent identity: range1484663
display id: range1484663
version: 1
direction: direction/forward
top level: BBa_F2622
start: 82
end: 837
orientation: inline

BBa_K1132020
type: ComponentDefinition
persistent identity: BBa K1132020
display id: BBa_K1132020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-YF1-rbs-FixJ-term-pFixK2-mRFP1
creator: iGEM Toulouse
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:09:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1444_
m user id: 0
owner id: 16633
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: later
mutable notes: later
mutable provenance: later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592006
component: BBa_B1010
component: BBa_K592016
component: BBa_K081014
sequence annotation: BBa_K592016
sequence annotation: BBa_K081014
sequence annotation: BBa_K592006
sequence annotation: BBa_B1010
sequence: https://synbiohub.org/public/igem/BBa_K1132020_sequence/1

BBa_K592006
type: Component
persistent identity: component2356556
display id: component2356556
version: 1
top level: BBa_K1132020
access: public
definition: https://synbiohub.org/public/igem/BBa_K592006/1

BBa_B1010
type: Component
persistent identity: component2356554
display id: component2356554
version: 1
top level: BBa_K1132020
access: public
definition: https://synbiohub.org/public/igem/BBa_B1010/1

BBa_K592016
type: Component
persistent identity: component2356549
display id: component2356549
version: 1
top level: BBa_K1132020
access: public
definition: https://synbiohub.org/public/igem/BBa_K592016/1

BBa_K081014
type: Component
persistent identity: component2356568
display id: component2356568
version: 1
top level: BBa_K1132020
access: public
definition: https://synbiohub.org/public/igem/BBa_K081014/1

BBa_K592016
type: SequenceAnnotation
persistent identity: annotation2356549
display id: annotation2356549
version: 1
top level: BBa_K1132020
location: https://synbiohub.org/public/igem/BBa_K1132020/annotation2356549/range2356549/1
component: BBa_K592016

https://synbiohub.org/public/igem/BBa_K1132020/annotation2356549/range2356549/1
type: Range
persistent identity: range2356549
display id: range2356549
version: 1
direction: direction/reverse
top level: BBa_K1132020
start: 1
end: 1796
orientation: inline

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2356568
display id: annotation2356568
version: 1
top level: BBa_K1132020
location: https://synbiohub.org/public/igem/BBa_K1132020/annotation2356568/range2356568/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K1132020/annotation2356568/range2356568/1
type: Range
persistent identity: range2356568
display id: range2356568
version: 1
direction: direction/reverse
top level: BBa_K1132020
start: 2111
end: 2884
orientation: inline

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2356556
display id: annotation2356556
version: 1
top level: BBa_K1132020
location: https://synbiohub.org/public/igem/BBa_K1132020/annotation2356556/range2356556/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_K1132020/annotation2356556/range2356556/1
type: Range
persistent identity: range2356556
display id: range2356556
version: 1
direction: direction/reverse
top level: BBa_K1132020
start: 1853
end: 2102
orientation: inline

BBa_B1010
type: SequenceAnnotation
persistent identity: annotation2356554
display id: annotation2356554
version: 1
top level: BBa_K1132020
location: https://synbiohub.org/public/igem/BBa_K1132020/annotation2356554/range2356554/1
component: BBa_B1010

https://synbiohub.org/public/igem/BBa_K1132020/annotation2356554/range2356554/1
type: Range
persistent identity: range2356554
display id: range2356554
version: 1
direction: direction/reverse
top level: BBa_K1132020
start: 1805
end: 1844
orientation: inline

BBa_K1742004
type: ComponentDefinition
persistent identity: BBa K1742004
display id: BBa_K1742004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhiC31 Plant codon optimized
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2015-12-16T01:52:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: It is a CDS of PhiC31 integrase from Streptomyces phage. It has a nuclear location sequence in the N-terminus.
mutable notes: It has a nuclear location sequence (NLS) and its codons are optimized for plants.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742004_sequence/1

BBa_J23117
type: ComponentDefinition
persistent identity: BBa J23117
display id: BBa_J23117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23117_sequence/1

BBa_K1390004
type: ComponentDefinition
persistent identity: BBa K1390004
display id: BBa_K1390004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mmoX
creator: Stefan D??bel
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mmoX is one subunit of the mmo from Methylococcus capsulatus bath
mutable notes: the brick is constructed in the RCF10 standard
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath without any PstI restriction site
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390004_sequence/1

BBa_S03154
type: ComponentDefinition
persistent identity: BBa S03154
display id: BBa_S03154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0078
creator: Jennifer Braff
created: 2004-07-22T11:00:00Z
modified: 2015-05-08T01:14:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0078
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0078
sequence: https://synbiohub.org/public/igem/BBa_S03154_sequence/1

BBa_C0078
type: Component
persistent identity: component940972
display id: component940972
version: 1
top level: BBa_S03154
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_B0034
type: Component
persistent identity: component940962
display id: component940962
version: 1
top level: BBa_S03154
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation940962
display id: annotation940962
version: 1
top level: BBa_S03154
location: https://synbiohub.org/public/igem/BBa_S03154/annotation940962/range940962/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03154/annotation940962/range940962/1
type: Range
persistent identity: range940962
display id: range940962
version: 1
direction: direction/forward
top level: BBa_S03154
start: 1
end: 12
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation940972
display id: annotation940972
version: 1
top level: BBa_S03154
location: https://synbiohub.org/public/igem/BBa_S03154/annotation940972/range940972/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_S03154/annotation940972/range940972/1
type: Range
persistent identity: range940972
display id: range940972
version: 1
direction: direction/forward
top level: BBa_S03154
start: 19
end: 660
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: BBa_R1062
sequence annotation: -35
sequence annotation: LuxR/HSL
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
direction: direction/forward
top level: lux pR
start: 54
end: 54
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 48
orientation: inline

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
creator: jcbraff
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP (mut3b) [note that this part does not have a barcode]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
direction: direction/forward
top level: GFP
start: 1
end: 720
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

BBa_J23070
type: ComponentDefinition
persistent identity: BBa J23070
display id: BBa_J23070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ser2 derived key
creator: bryan hernandez
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 936
owning group id: 52
sample status: In stock
status: status/Available
bookmark: true
mutable description: contains the J23060 loop on a tRNA. more on this later
mutable notes: no
mutable provenance: Ser2 tRNA and J23060
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: binding site
sequence annotation: loop
sequence: https://synbiohub.org/public/igem/BBa_J23070_sequence/1

binding site
type: SequenceAnnotation
persistent identity: annotation1897362
display id: annotation1897362
version: 1
top level: BBa_J23070
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J23070/annotation1897362/range1897362/1

https://synbiohub.org/public/igem/BBa_J23070/annotation1897362/range1897362/1
type: Range
persistent identity: range1897362
display id: range1897362
version: 1
direction: direction/forward
top level: BBa_J23070
start: 43
end: 69
orientation: inline

loop
type: SequenceAnnotation
persistent identity: annotation1897361
display id: annotation1897361
version: 1
top level: BBa_J23070
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J23070/annotation1897361/range1897361/1

https://synbiohub.org/public/igem/BBa_J23070/annotation1897361/range1897361/1
type: Range
persistent identity: range1897361
display id: range1897361
version: 1
direction: direction/forward
top level: BBa_J23070
start: 37
end: 74
orientation: inline

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spacer.1 (generic)
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BBa_B0040
sequence annotation: Spacer-1
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
direction: direction/forward
top level: spacer
start: 1
end: 70
orientation: inline

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
direction: direction/forward
top level: spacer
start: 1
end: 70
orientation: inline

BBa_K1114000
type: ComponentDefinition
persistent identity: BBa K1114000
display id: BBa_K1114000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23100 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part.
mutable notes: This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part. Summary of Modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114000_sequence/1

rib-synth
type: ComponentDefinition
persistent identity: BBa K1172303
display id: BBa_K1172303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboflavin synthesis gene cluster from shewanella oneidensis
creator: Thorben Meyer
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 17241
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This gene cluster consists of four different genes that form a single operon.
mutable notes: we eliminated three illegal restriction sites.
mutable provenance: genome of shewanella oneidensis MR-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SO_3468
sequence annotation: taa
sequence annotation: atg
sequence annotation: Pst1 C->T
sequence annotation: tga
sequence annotation: ribBA
sequence annotation: ribE
sequence annotation: ribD
sequence annotation: EcoR1 C->T
sequence annotation: tag
sequence: https://synbiohub.org/public/igem/BBa_K1172303_sequence/1

SO_3468
type: SequenceAnnotation
persistent identity: annotation2370311
display id: annotation2370311
version: 1
top level: rib-synth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370311/range2370311/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370311/range2370311/1
type: Range
persistent identity: range2370311
display id: range2370311
version: 1
direction: direction/forward
top level: rib-synth
start: 1154
end: 1810
orientation: inline

taa
type: SequenceAnnotation
persistent identity: annotation2370723
display id: annotation2370723
version: 1
top level: rib-synth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370723/range2370723/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370723/range2370723/1
type: Range
persistent identity: range2370723
display id: range2370723
version: 1
direction: direction/forward
top level: rib-synth
start: 2978
end: 2980
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2370720
display id: annotation2370720
version: 1
top level: rib-synth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370720/range2370720/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370720/range2370720/1
type: Range
persistent identity: range2370720
display id: range2370720
version: 1
direction: direction/forward
top level: rib-synth
start: 3121
end: 3123
orientation: inline

Pst1 C->T
type: SequenceAnnotation
persistent identity: annotation2370714
display id: annotation2370714
version: 1
top level: rib-synth
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370714/range2370714/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370714/range2370714/1
type: Range
persistent identity: range2370714
display id: range2370714
version: 1
direction: direction/forward
top level: rib-synth
start: 234
end: 239
orientation: inline

tga
type: SequenceAnnotation
persistent identity: annotation2370721
display id: annotation2370721
version: 1
top level: rib-synth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370721/range2370721/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370721/range2370721/1
type: Range
persistent identity: range2370721
display id: range2370721
version: 1
direction: direction/forward
top level: rib-synth
start: 1144
end: 1146
orientation: inline

Pst1 C->T
type: SequenceAnnotation
persistent identity: annotation2370715
display id: annotation2370715
version: 1
top level: rib-synth
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370715/range2370715/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370715/range2370715/1
type: Range
persistent identity: range2370715
display id: range2370715
version: 1
direction: direction/forward
top level: rib-synth
start: 1644
end: 1649
orientation: inline

ribBA
type: SequenceAnnotation
persistent identity: annotation2370312
display id: annotation2370312
version: 1
top level: rib-synth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370312/range2370312/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370312/range2370312/1
type: Range
persistent identity: range2370312
display id: range2370312
version: 1
direction: direction/forward
top level: rib-synth
start: 1877
end: 2980
orientation: inline

ribE
type: SequenceAnnotation
persistent identity: annotation2370313
display id: annotation2370313
version: 1
top level: rib-synth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370313/range2370313/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370313/range2370313/1
type: Range
persistent identity: range2370313
display id: range2370313
version: 1
direction: direction/forward
top level: rib-synth
start: 3121
end: 3597
orientation: inline

ribD
type: SequenceAnnotation
persistent identity: annotation2370310
display id: annotation2370310
version: 1
top level: rib-synth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370310/range2370310/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370310/range2370310/1
type: Range
persistent identity: range2370310
display id: range2370310
version: 1
direction: direction/forward
top level: rib-synth
start: 1
end: 1146
orientation: inline

EcoR1 C->T
type: SequenceAnnotation
persistent identity: annotation2370716
display id: annotation2370716
version: 1
top level: rib-synth
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370716/range2370716/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370716/range2370716/1
type: Range
persistent identity: range2370716
display id: range2370716
version: 1
direction: direction/forward
top level: rib-synth
start: 1840
end: 1845
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2370719
display id: annotation2370719
version: 1
top level: rib-synth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370719/range2370719/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370719/range2370719/1
type: Range
persistent identity: range2370719
display id: range2370719
version: 1
direction: direction/forward
top level: rib-synth
start: 1877
end: 1879
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2370718
display id: annotation2370718
version: 1
top level: rib-synth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370718/range2370718/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370718/range2370718/1
type: Range
persistent identity: range2370718
display id: range2370718
version: 1
direction: direction/forward
top level: rib-synth
start: 1154
end: 1156
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2370717
display id: annotation2370717
version: 1
top level: rib-synth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370717/range2370717/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370717/range2370717/1
type: Range
persistent identity: range2370717
display id: range2370717
version: 1
direction: direction/forward
top level: rib-synth
start: 1
end: 3
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2370722
display id: annotation2370722
version: 1
top level: rib-synth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370722/range2370722/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370722/range2370722/1
type: Range
persistent identity: range2370722
display id: range2370722
version: 1
direction: direction/forward
top level: rib-synth
start: 1808
end: 1810
orientation: inline

taa
type: SequenceAnnotation
persistent identity: annotation2370724
display id: annotation2370724
version: 1
top level: rib-synth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370724/range2370724/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370724/range2370724/1
type: Range
persistent identity: range2370724
display id: range2370724
version: 1
direction: direction/forward
top level: rib-synth
start: 3595
end: 3597
orientation: inline

norB
type: ComponentDefinition
persistent identity: BBa K1356005
display id: BBa_K1356005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitric oxide reductase (norB) from Pseudomonas aeruginosa PAO1
creator: Cameron Sargent
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1732_
m user id: 0
owner id: 20075
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Blah
mutable notes: Mutagenesis
mutable provenance: P.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1356005_sequence/1

BBa_K737016
type: ComponentDefinition
persistent identity: BBa K737016
display id: BBa_K737016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This part conteins gvpA protein's coding sequence.
creator: Jiaheng Li
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part conteins gvpA protein's coding sequence. This gene from Planktothrix rubescens can be succesfully expressed in E. coli. The gvpA's expression have been examined by another part " J23106+B0034+gvpA+B0015". (Since it was ligated on cloning vector pSB4A5, we didn't register this part. The gvpA protein conteins a typical domain of the superfamily 'Gas vesicle'(NCBI CDD cl02594). This gene is quiet conserved in most gvp's polycistron. According to Anthony E. Walsby's research, gas vesicles have a similar morphology. GvpA protein is a kind of small hydrophobic protein which forms the ribs of the main structure. 'All cyanobacterial gas vesicles so far analyzed contain a protein (GvpA) of about 7.4 kDa that forms the main mass of the structure and must be responsible for many of its properties.' (Gas Vesicles. Microbiological Reviews. A. E. Walsby)
mutable notes: According to Anthony E. Walsby's research, gas vesicles have a similar morphology. GvpA protein is a kind of small hydrophobic protein which forms the ribs of the main structure. 'All cyanobacterial gas vesicles so far analyzed contain a protein (GvpA) of about 7.4 kDa that forms the main mass of the structure and must be responsible for many of its properties.' (Gas Vesicles. Microbiological Reviews. A. E. Walsby)
mutable provenance: Yes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K737016_sequence/1

BBa_K1114101
type: ComponentDefinition
persistent identity: BBa K1114101
display id: BBa_K1114101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo format of a modified version of BBa_B0031 with BC fusion sites.
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a modified BBa_B0031 with BC fusion sites. See the BBa_B0031 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of a modified BBa_B0031 with BC fusion sites. See the BBa_B0031 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114101_sequence/1

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (p22 cII regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: OR3
sequence annotation: BBa_R0053
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
direction: direction/forward
top level: cII p22
start: 34
end: 51
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
direction: direction/forward
top level: cII p22
start: 11
end: 28
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 3
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
direction: direction/forward
top level: cII p22
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
direction: direction/forward
top level: cII p22
start: 30
end: 35
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
direction: direction/forward
top level: cII p22
start: 8
end: 13
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

BBa_K783024
type: ComponentDefinition
persistent identity: BBa K783024
display id: BBa_K783024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_R0063
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo converted version of BBa_R0063, which is the negatively regulated pLuxR promoter. It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_R0063
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: Putative LuxR/HSL
sequence annotation: -10
sequence annotation: BBa_R0063
sequence annotation: LuxR/HSL
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K783024_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2214224
display id: annotation2214224
version: 1
top level: BBa_K783024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214224/range2214224/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214224/range2214224/1
type: Range
persistent identity: range2214224
display id: range2214224
version: 1
direction: direction/forward
top level: BBa_K783024
start: 89
end: 94
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2214227
display id: annotation2214227
version: 1
top level: BBa_K783024
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214227/range2214227/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214227/range2214227/1
type: Range
persistent identity: range2214227
display id: range2214227
version: 1
direction: direction/forward
top level: BBa_K783024
start: 130
end: 149
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2214225
display id: annotation2214225
version: 1
top level: BBa_K783024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214225/range2214225/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214225/range2214225/1
type: Range
persistent identity: range2214225
display id: range2214225
version: 1
direction: direction/forward
top level: BBa_K783024
start: 115
end: 122
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2214223
display id: annotation2214223
version: 1
top level: BBa_K783024
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214223/range2214223/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214223/range2214223/1
type: Range
persistent identity: range2214223
display id: range2214223
version: 1
direction: direction/forward
top level: BBa_K783024
start: 1
end: 151
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2214222
display id: annotation2214222
version: 1
top level: BBa_K783024
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214222/range2214222/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214222/range2214222/1
type: Range
persistent identity: range2214222
display id: range2214222
version: 1
direction: direction/forward
top level: BBa_K783024
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2214226
display id: annotation2214226
version: 1
top level: BBa_K783024
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K783024/annotation2214226/range2214226/1

https://synbiohub.org/public/igem/BBa_K783024/annotation2214226/range2214226/1
type: Range
persistent identity: range2214226
display id: range2214226
version: 1
direction: direction/forward
top level: BBa_K783024
start: 128
end: 128
orientation: inline

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PenI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
mutable notes: extends slightly past -50
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: dimer right half
sequence annotation: -35
sequence annotation: PenI
sequence annotation: dimer left half
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
direction: direction/forward
top level: PenI
start: 55
end: 76
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
direction: direction/forward
top level: PenI
start: 23
end: 28
orientation: inline

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
direction: direction/forward
top level: PenI
start: 1
end: 77
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
direction: direction/forward
top level: PenI
start: 31
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
direction: direction/forward
top level: PenI
start: 46
end: 51
orientation: inline

BBa_K1033914
type: ComponentDefinition
persistent identity: BBa K1033914
display id: BBa_K1033914
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein (incl RBS & J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia majano
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23110
component: BBa_K1033916
component: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_K1033916
sequence: https://synbiohub.org/public/igem/BBa_K1033914_sequence/1

BBa_J23110
type: Component
persistent identity: component2350103
display id: component2350103
version: 1
top level: BBa_K1033914
access: public
definition: BBa_J23110

BBa_K1033916
type: Component
persistent identity: component2350107
display id: component2350107
version: 1
top level: BBa_K1033914
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033916/1

BBa_B0034
type: Component
persistent identity: component2350105
display id: component2350105
version: 1
top level: BBa_K1033914
access: public
definition: BBa_B0034

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2350103
display id: annotation2350103
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350103/range2350103/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350103/range2350103/1
type: Range
persistent identity: range2350103
display id: range2350103
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2350105
display id: annotation2350105
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350105/range2350105/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350105/range2350105/1
type: Range
persistent identity: range2350105
display id: range2350105
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 44
end: 55
orientation: inline

BBa_K1033916
type: SequenceAnnotation
persistent identity: annotation2350107
display id: annotation2350107
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350107/range2350107/1
component: BBa_K1033916

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350107/range2350107/1
type: Range
persistent identity: range2350107
display id: range2350107
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 62
end: 754
orientation: inline

BBa_K801041
type: ComponentDefinition
persistent identity: BBa K801041
display id: BBa_K801041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-PhyB-linker-LexA (part for a LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033
mutable notes: -
mutable provenance: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K801036
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: BBa_K801030
sequence annotation: RFC25 fusion scar
sequence annotation: BBa_K801031
sequence annotation: BBa_K801033
sequence annotation: BBa_K365005
sequence: https://synbiohub.org/public/igem/BBa_K801041_sequence/1

BBa_K801036
type: SequenceAnnotation
persistent identity: annotation2199718
display id: annotation2199718
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199718/range2199718/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199718/range2199718/1
type: Range
persistent identity: range2199718
display id: range2199718
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2791
end: 3459
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199712
display id: annotation2199712
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199712/range2199712/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199712/range2199712/1
type: Range
persistent identity: range2199712
display id: range2199712
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1114
end: 1116
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199707
display id: annotation2199707
version: 1
top level: BBa_K801041
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199707/range2199707/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199707/range2199707/1
type: Range
persistent identity: range2199707
display id: range2199707
version: 1
direction: direction/forward
top level: BBa_K801041
start: 22
end: 42
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199710
display id: annotation2199710
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199710/range2199710/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199710/range2199710/1
type: Range
persistent identity: range2199710
display id: range2199710
version: 1
direction: direction/forward
top level: BBa_K801041
start: 391
end: 393
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2199713
display id: annotation2199713
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199713/range2199713/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199713/range2199713/1
type: Range
persistent identity: range2199713
display id: range2199713
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1129
end: 1131
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199709
display id: annotation2199709
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199709/range2199709/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199709/range2199709/1
type: Range
persistent identity: range2199709
display id: range2199709
version: 1
direction: direction/forward
top level: BBa_K801041
start: 388
end: 390
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199711
display id: annotation2199711
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199711/range2199711/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199711/range2199711/1
type: Range
persistent identity: range2199711
display id: range2199711
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1102
end: 1104
orientation: inline

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2199708
display id: annotation2199708
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199708/range2199708/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199708/range2199708/1
type: Range
persistent identity: range2199708
display id: range2199708
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1
end: 57
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199716
display id: annotation2199716
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199716/range2199716/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199716/range2199716/1
type: Range
persistent identity: range2199716
display id: range2199716
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2851
end: 2856
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2199714
display id: annotation2199714
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199714/range2199714/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199714/range2199714/1
type: Range
persistent identity: range2199714
display id: range2199714
version: 1
direction: direction/forward
top level: BBa_K801041
start: 64
end: 2784
orientation: inline

BBa_K801033
type: SequenceAnnotation
persistent identity: annotation2199717
display id: annotation2199717
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199717/range2199717/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199717/range2199717/1
type: Range
persistent identity: range2199717
display id: range2199717
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2857
end: 3459
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2199715
display id: annotation2199715
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199715/range2199715/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199715/range2199715/1
type: Range
persistent identity: range2199715
display id: range2199715
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2791
end: 2851
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199724
display id: annotation2199724
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199724/range2199724/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199724/range2199724/1
type: Range
persistent identity: range2199724
display id: range2199724
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2785
end: 2790
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199722
display id: annotation2199722
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199722/range2199722/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199722/range2199722/1
type: Range
persistent identity: range2199722
display id: range2199722
version: 1
direction: direction/forward
top level: BBa_K801041
start: 58
end: 63
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from E. coli phage lambda (+LVA)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
direction: direction/forward
top level: cI lam
start: 712
end: 744
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
direction: direction/forward
top level: cI lam
start: 4
end: 711
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
direction: direction/forward
top level: cI lam
start: 751
end: 775
orientation: inline

R6K
type: ComponentDefinition
persistent identity: BBa J61001
display id: BBa_J61001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [R6K] Origin of replication
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011}
mutable notes: N/A
mutable provenance: PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is Bca1011 ---- ca1011F Forward Biobricking of R6K GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg ca1011R Reverse biobricking of R6K ccgctactagtaGCAGTTCAACCTGTTGATAG
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K
sequence: https://synbiohub.org/public/igem/BBa_J61001_sequence/1

R6K
type: SequenceAnnotation
persistent identity: annotation1892271
display id: annotation1892271
version: 1
top level: R6K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1

https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
type: Range
persistent identity: range1892271
display id: range1892271
version: 1
direction: direction/forward
top level: R6K
start: 27
end: 406
orientation: inline

BBa_K731500
type: ComponentDefinition
persistent identity: BBa K731500
display id: BBa_K731500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward
creator: Francesco Guzzonato
created: 2012-08-21T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12142
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is E.coli lactose-inducibile promoter. lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow.
mutable provenance: pET21b
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: rbs
sequence annotation: start
sequence annotation: BBa_K731300
sequence annotation: lacO operator
sequence annotation: Ptac promoter
sequence annotation: stop
sequence annotation: LacI
sequence annotation: LacIq promoter
sequence: https://synbiohub.org/public/igem/BBa_K731500_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2181542
display id: annotation2181542
version: 1
top level: BBa_K731500
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K731500/annotation2181542/range2181542/1

https://synbiohub.org/public/igem/BBa_K731500/annotation2181542/range2181542/1
type: Range
persistent identity: range2181542
display id: range2181542
version: 1
direction: direction/forward
top level: BBa_K731500
start: 1085
end: 1092
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2181541
display id: annotation2181541
version: 1
top level: BBa_K731500
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K731500/annotation2181541/range2181541/1

https://synbiohub.org/public/igem/BBa_K731500/annotation2181541/range2181541/1
type: Range
persistent identity: range2181541
display id: range2181541
version: 1
direction: direction/reverse
top level: BBa_K731500
start: 1081
end: 1084
orientation: inline

BBa_K731300
type: SequenceAnnotation
persistent identity: annotation2181544
display id: annotation2181544
version: 1
top level: BBa_K731500
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K731500/annotation2181544/range2181544/1

https://synbiohub.org/public/igem/BBa_K731500/annotation2181544/range2181544/1
type: Range
persistent identity: range2181544
display id: range2181544
version: 1
direction: direction/reverse
top level: BBa_K731500
start: 1
end: 1153
orientation: inline

lacO operator
type: SequenceAnnotation
persistent identity: annotation2181545
display id: annotation2181545
version: 1
top level: BBa_K731500
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K731500/annotation2181545/range2181545/1

https://synbiohub.org/public/igem/BBa_K731500/annotation2181545/range2181545/1
type: Range
persistent identity: range2181545
display id: range2181545
version: 1
direction: direction/forward
top level: BBa_K731500
start: 1194
end: 1219
orientation: inline

Ptac promoter
type: SequenceAnnotation
persistent identity: annotation2181546
display id: annotation2181546
version: 1
top level: BBa_K731500
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731500/annotation2181546/range2181546/1

https://synbiohub.org/public/igem/BBa_K731500/annotation2181546/range2181546/1
type: Range
persistent identity: range2181546
display id: range2181546
version: 1
direction: direction/forward
top level: BBa_K731500
start: 1162
end: 1188
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2181539
display id: annotation2181539
version: 1
top level: BBa_K731500
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K731500/annotation2181539/range2181539/1

https://synbiohub.org/public/igem/BBa_K731500/annotation2181539/range2181539/1
type: Range
persistent identity: range2181539
display id: range2181539
version: 1
direction: direction/reverse
top level: BBa_K731500
start: 1
end: 3
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2181540
display id: annotation2181540
version: 1
top level: BBa_K731500
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K731500/annotation2181540/range2181540/1

https://synbiohub.org/public/igem/BBa_K731500/annotation2181540/range2181540/1
type: Range
persistent identity: range2181540
display id: range2181540
version: 1
direction: direction/reverse
top level: BBa_K731500
start: 1
end: 1084
orientation: inline

LacIq promoter
type: SequenceAnnotation
persistent identity: annotation2181543
display id: annotation2181543
version: 1
top level: BBa_K731500
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731500/annotation2181543/range2181543/1

https://synbiohub.org/public/igem/BBa_K731500/annotation2181543/range2181543/1
type: Range
persistent identity: range2181543
display id: range2181543
version: 1
direction: direction/reverse
top level: BBa_K731500
start: 1093
end: 1153
orientation: inline

asPink
type: ComponentDefinition
persistent identity: BBa K1033927
display id: BBa_K1033927
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asPink, pink chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia sulcata, asCP, naturally exhibits color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia sulcata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033933
component: BBa_B0034
sequence annotation: BBa_K1033933
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033927_sequence/1

BBa_K1033933
type: Component
persistent identity: component2379051
display id: component2379051
version: 1
top level: asPink
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033933/1

BBa_B0034
type: Component
persistent identity: component2379049
display id: component2379049
version: 1
top level: asPink
access: public
definition: BBa_B0034

BBa_K1033933
type: SequenceAnnotation
persistent identity: annotation2379051
display id: annotation2379051
version: 1
top level: asPink
location: https://synbiohub.org/public/igem/BBa_K1033927/annotation2379051/range2379051/1
component: BBa_K1033933

https://synbiohub.org/public/igem/BBa_K1033927/annotation2379051/range2379051/1
type: Range
persistent identity: range2379051
display id: range2379051
version: 1
direction: direction/reverse
top level: asPink
start: 19
end: 720
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2379049
display id: annotation2379049
version: 1
top level: asPink
location: https://synbiohub.org/public/igem/BBa_K1033927/annotation2379049/range2379049/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033927/annotation2379049/range2379049/1
type: Range
persistent identity: range2379049
display id: range2379049
version: 1
direction: direction/reverse
top level: asPink
start: 1
end: 12
orientation: inline

BBa_S0105
type: ComponentDefinition
persistent identity: BBa S0105
display id: BBa_S0105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0053
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_C0053
sequence annotation: BBa_B0034
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_S0105_sequence/1

BBa_B0034
type: Component
persistent identity: component939777
display id: component939777
version: 1
top level: BBa_S0105
access: public
definition: BBa_B0034

BBa_C0053
type: Component
persistent identity: component939789
display id: component939789
version: 1
top level: BBa_S0105
access: public
definition: c2 P22

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939777
display id: annotation939777
version: 1
top level: BBa_S0105
location: https://synbiohub.org/public/igem/BBa_S0105/annotation939777/range939777/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0105/annotation939777/range939777/1
type: Range
persistent identity: range939777
display id: range939777
version: 1
direction: direction/forward
top level: BBa_S0105
start: 1
end: 12
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation939789
display id: annotation939789
version: 1
top level: BBa_S0105
location: https://synbiohub.org/public/igem/BBa_S0105/annotation939789/range939789/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_S0105/annotation939789/range939789/1
type: Range
persistent identity: range939789
display id: range939789
version: 1
direction: direction/forward
top level: BBa_S0105
start: 19
end: 705
orientation: inline

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.3 (medium) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0032
sequence annotation: RBS-3\Weak
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
direction: direction/forward
top level: BBa_B0032
start: 7
end: 10
orientation: inline

BBa_C0056
type: ComponentDefinition
persistent identity: BBa C0056
display id: BBa_C0056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (no LVA)
creator: Reshma Shetty
created: 2004-09-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Removed LVA tag from BBa_C0052 via PCR. In pSB1AK3 currently.
mutable provenance: Removed LVA tag from BBa_C0052
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI 434
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C0056_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation1063931
display id: annotation1063931
version: 1
top level: BBa_C0056
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
type: Range
persistent identity: range1063931
display id: range1063931
version: 1
direction: direction/forward
top level: BBa_C0056
start: 1
end: 636
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1063930
display id: annotation1063930
version: 1
top level: BBa_C0056
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
type: Range
persistent identity: range1063930
display id: range1063930
version: 1
direction: direction/forward
top level: BBa_C0056
start: 631
end: 636
orientation: inline

BBa_K1216008
type: ComponentDefinition
persistent identity: BBa K1216008
display id: BBa_K1216008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Variant of the wild-type pLuxR promoter with lower sensitivity
creator: Meret Arter
created: 2013-10-29T12:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1530_
m user id: 0
owner id: 18108
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1216007 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.
mutable notes: LuxR binding site were mutated to reduce the sensitivity of the promoter.
mutable provenance: The promoter was built through site-saturation mutagenesis of the wild-type promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1216008_sequence/1

BBa_K797002
type: ComponentDefinition
persistent identity: BBa K797002
display id: BBa_K797002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TorA signal with RBS
creator: Tomohiro Nobeyama
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1053_
m user id: 0
owner id: 11588
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is coding N-terminal torA signal region. Proteins conjugated torA signal are recognized by Tat secretion pathway and they are secreted from cytoplasm to periplasm while maintaining their holdings. This parts include RBS so that you don't bother to insert new RBS between this signal and your promoter. ( Note: Depending on your purpose, you might use BBa_K797004 when you use this signal because wild type Tat pathway is relatively saturated easily.)
mutable notes: When you combine two iGEM part by ligation following cutting by Xbal and Spe1, a stop codon sometimes appear. But don't worry. We modified the sequence of Tat signal so that this signal can always combine your protein.
mutable provenance: This parts is derived from Escherichia coli???s genomic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: Modified torA with RBS
sequence: https://synbiohub.org/public/igem/BBa_K797002_sequence/1

Modified torA with RBS
type: SequenceAnnotation
persistent identity: annotation2195230
display id: annotation2195230
version: 1
top level: BBa_K797002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K797002/annotation2195230/range2195230/1

https://synbiohub.org/public/igem/BBa_K797002/annotation2195230/range2195230/1
type: Range
persistent identity: range2195230
display id: range2195230
version: 1
direction: direction/forward
top level: BBa_K797002
start: 1
end: 192
orientation: inline

BBa_K1114016
type: ComponentDefinition
persistent identity: BBa K1114016
display id: BBa_K1114016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23113 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_J23113 with AB fusion sites. See the BBa_J23113 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_J23113 with AB fusion sites. See the BBa_J23113 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114016_sequence/1

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0040
sequence annotation: TetR 2
sequence annotation: TetR 1
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

BBa_I13002
type: ComponentDefinition
persistent identity: BBa I13002
display id: BBa_I13002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS B0031 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_B0031
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_I13002_sequence/1

BBa_E0030
type: Component
persistent identity: component943454
display id: component943454
version: 1
top level: BBa_I13002
access: public
definition: eyfp

BBa_B0031
type: Component
persistent identity: component943452
display id: component943452
version: 1
top level: BBa_I13002
access: public
definition: BBa_B0031

BBa_B0010
type: Component
persistent identity: component943459
display id: component943459
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component943469
display id: component943469
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943459
display id: annotation943459
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943459/range943459/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13002/annotation943459/range943459/1
type: Range
persistent identity: range943459
display id: range943459
version: 1
direction: direction/forward
top level: BBa_I13002
start: 752
end: 831
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943469
display id: annotation943469
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943469/range943469/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13002/annotation943469/range943469/1
type: Range
persistent identity: range943469
display id: range943469
version: 1
direction: direction/forward
top level: BBa_I13002
start: 840
end: 880
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943454
display id: annotation943454
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943454/range943454/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13002/annotation943454/range943454/1
type: Range
persistent identity: range943454
display id: range943454
version: 1
direction: direction/forward
top level: BBa_I13002
start: 21
end: 743
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation943452
display id: annotation943452
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943452/range943452/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I13002/annotation943452/range943452/1
type: Range
persistent identity: range943452
display id: range943452
version: 1
direction: direction/forward
top level: BBa_I13002
start: 1
end: 14
orientation: inline

BBa_B0024
type: ComponentDefinition
persistent identity: BBa B0024
display id: BBa_B0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0012-B0011), reversed
creator: Caitlin Conboy
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0024_sequence/1

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0030.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
component: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
direction: direction/forward
top level: GFP genera
start: 838
end: 878
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
direction: direction/forward
top level: GFP genera
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
direction: direction/forward
top level: GFP genera
start: 22
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
direction: direction/forward
top level: GFP genera
start: 750
end: 829
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: F2 OmpR
sequence annotation: -10
sequence annotation: F3 OmpR
sequence annotation: -35
sequence annotation: F1 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
direction: direction/forward
top level: OmpR
start: 31
end: 48
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
direction: direction/forward
top level: OmpR
start: 88
end: 93
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
direction: direction/forward
top level: OmpR
start: 51
end: 68
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
direction: direction/forward
top level: OmpR
start: 73
end: 78
orientation: inline

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
direction: direction/forward
top level: OmpR
start: 11
end: 28
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R1051
sequence annotation: OR1
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

BBa_K1114103
type: ComponentDefinition
persistent identity: BBa K1114103
display id: BBa_K1114103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo formatted version of B0033 with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_B0033 with BC fusion sites. See the BBa_B0033 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of BBa_B0033 with BC fusion sites. See the BBa_B0033 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114103_sequence/1

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (AraC regulated)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp1
sequence annotation: c-amp2
sequence annotation: -10
sequence annotation: -35
sequence annotation: araO1
sequence annotation: ara1 and ara2
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
direction: direction/forward
top level: AraC
start: 43
end: 72
orientation: inline

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
direction: direction/forward
top level: AraC
start: 4
end: 29
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
direction: direction/forward
top level: AraC
start: 136
end: 141
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
direction: direction/forward
top level: AraC
start: 113
end: 118
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
direction: direction/forward
top level: AraC
start: 6
end: 44
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
direction: direction/forward
top level: AraC
start: 73
end: 101
orientation: inline

BBa_K1114021
type: ComponentDefinition
persistent identity: BBa K1114021
display id: BBa_K1114021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23118 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_J23118 with AB fusion sites. See the BBa_J23118 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_J23118 with AB fusion sites. See the BBa_J23118 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1114021_sequence/1

ECFP
type: ComponentDefinition
persistent identity: BBa E0024
display id: BBa_E0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0024_sequence/1

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

BBa_K115003
type: ComponentDefinition
persistent identity: BBa K115003
display id: BBa_K115003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (PrfA)
creator: O.M.J.A. Stassen
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3007
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is designed as a temperature inducible RBS. Only designed so far.
mutable notes: The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
mutable provenance: This sequence is taken from the Listeria Monocytogenes (EU372032.). It's the 5'UTR of a TF induced at 37 degrees.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Predicted stem loop up to A of AUG
sequence annotation: SD
sequence: https://synbiohub.org/public/igem/BBa_K115003_sequence/1

Predicted stem loop up to A of AUG
type: SequenceAnnotation
persistent identity: annotation1967003
display id: annotation1967003
version: 1
top level: BBa_K115003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115003/annotation1967003/range1967003/1

https://synbiohub.org/public/igem/BBa_K115003/annotation1967003/range1967003/1
type: Range
persistent identity: range1967003
display id: range1967003
version: 1
direction: direction/forward
top level: BBa_K115003
start: 28
end: 109
orientation: inline

SD
type: SequenceAnnotation
persistent identity: annotation1966983
display id: annotation1966983
version: 1
top level: BBa_K115003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115003/annotation1966983/range1966983/1

https://synbiohub.org/public/igem/BBa_K115003/annotation1966983/range1966983/1
type: Range
persistent identity: range1966983
display id: range1966983
version: 1
direction: direction/forward
top level: BBa_K115003
start: 106
end: 109
orientation: inline

BBa_K1357009
type: ComponentDefinition
persistent identity: BBa K1357009
display id: BBa_K1357009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP Reporter (RBS+Coding+Term)
creator: Matthew Tucker
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _1733_
m user id: 0
owner id: 16772
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Show functionality of gene circuit
mutable notes: None
mutable provenance: jellyfish
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K592009
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K592009
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1357009_sequence/1

BBa_B0034
type: Component
persistent identity: component2408702
display id: component2408702
version: 1
top level: BBa_K1357009
access: public
definition: BBa_B0034

BBa_K592009
type: Component
persistent identity: component2408704
display id: component2408704
version: 1
top level: BBa_K1357009
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_B0015
type: Component
persistent identity: component2408711
display id: component2408711
version: 1
top level: BBa_K1357009
access: public
definition: BBa_B0015

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2408711
display id: annotation2408711
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408711/range2408711/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408711/range2408711/1
type: Range
persistent identity: range2408711
display id: range2408711
version: 1
direction: direction/reverse
top level: BBa_K1357009
start: 696
end: 824
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2408704
display id: annotation2408704
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408704/range2408704/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408704/range2408704/1
type: Range
persistent identity: range2408704
display id: range2408704
version: 1
direction: direction/reverse
top level: BBa_K1357009
start: 19
end: 687
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2408702
display id: annotation2408702
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408702/range2408702/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408702/range2408702/1
type: Range
persistent identity: range2408702
display id: range2408702
version: 1
direction: direction/reverse
top level: BBa_K1357009
start: 1
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0010-B0012)
creator: Reshma Shetty
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
direction: direction/forward
top level: BBa_B0015
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
direction: direction/forward
top level: BBa_B0015
start: 89
end: 129
orientation: inline

ATF1 gener
type: ComponentDefinition
persistent identity: BBa J45199
display id: BBa_J45199
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor enzyme (ATF1) generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45200.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_J45014
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J45014
sequence: https://synbiohub.org/public/igem/BBa_J45199_sequence/1

BBa_B0030
type: Component
persistent identity: component1908009
display id: component1908009
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45014
type: Component
persistent identity: component1908015
display id: component1908015
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_J45014/1

BBa_B0010
type: Component
persistent identity: component1908016
display id: component1908016
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1908018
display id: component1908018
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908016
display id: annotation1908016
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
type: Range
persistent identity: range1908016
display id: range1908016
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1611
end: 1690
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908018
display id: annotation1908018
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
type: Range
persistent identity: range1908018
display id: range1908018
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1699
end: 1739
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1908009
display id: annotation1908009
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
type: Range
persistent identity: range1908009
display id: range1908009
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1
end: 15
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908015
display id: annotation1908015
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
type: Range
persistent identity: range1908015
display id: range1908015
version: 1
direction: direction/reverse
top level: ATF1 gener
start: 22
end: 1602
orientation: inline

BBa_K352003
type: ComponentDefinition
persistent identity: BBa K352003
display id: BBa_K352003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCooM Promoter from Rhodospirillum rubrum
creator: Ceren Seref
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _474_
m user id: 0
owner id: 5815
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The two CooA-regulated R. rubrum promoters, PcooF and PcooM, contain 2-fold symmetric DNA sequences that serve as CooA-binding sites and are similar to the CRP consensus sequence. This is consistent with the similarity between CooA and CRP in their helix-turn-helix motifs. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo. The CO-dependent anaerobic growth of Rhodospirillum rubrum relies on a CO oxidation system encoded by two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ. The key products of the coo regulon are an O2- sensitive CO dehydrogenase (CooS), a CooS-associated Fe-S protein (CooF), and a CO-tolerant hydrogenase (CooH), and the expression of the genes depends upon the activity of the CooA protein, which senses CO under anaerobic conditions.
mutable notes: The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.
mutable provenance: Y. He, T. Gaal, R. Karls, T. Donohue, R. Gourse and G.P. Roberts, J. Biol. Chem. 274 (1999), pp. 10840???10846.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CooA binding Site
sequence: https://synbiohub.org/public/igem/BBa_K352003_sequence/1

CooA binding Site
type: SequenceAnnotation
persistent identity: annotation2081260
display id: annotation2081260
version: 1
top level: BBa_K352003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K352003/annotation2081260/range2081260/1

https://synbiohub.org/public/igem/BBa_K352003/annotation2081260/range2081260/1
type: Range
persistent identity: range2081260
display id: range2081260
version: 1
direction: direction/forward
top level: BBa_K352003
start: 25
end: 29
orientation: inline

CooA binding Site
type: SequenceAnnotation
persistent identity: annotation2081259
display id: annotation2081259
version: 1
top level: BBa_K352003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K352003/annotation2081259/range2081259/1

https://synbiohub.org/public/igem/BBa_K352003/annotation2081259/range2081259/1
type: Range
persistent identity: range2081259
display id: range2081259
version: 1
direction: direction/forward
top level: BBa_K352003
start: 14
end: 18
orientation: inline

BBa_K1104300
type: ComponentDefinition
persistent identity: BBa K1104300
display id: BBa_K1104300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Abaecin
creator: Jou-Chien Liao, Rohan Shina, Yucheng Huang
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1415_
m user id: 0
owner id: 16321
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Abaecin
mutable notes: None
mutable provenance: ''Apis melliferra''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Term
sequence annotation: mature peptide
sequence annotation: Methionine
sequence: https://synbiohub.org/public/igem/BBa_K1104300_sequence/1

Term
type: SequenceAnnotation
persistent identity: annotation2363580
display id: annotation2363580
version: 1
top level: BBa_K1104300
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1104300/annotation2363580/range2363580/1

https://synbiohub.org/public/igem/BBa_K1104300/annotation2363580/range2363580/1
type: Range
persistent identity: range2363580
display id: range2363580
version: 1
direction: direction/forward
top level: BBa_K1104300
start: 106
end: 108
orientation: inline

mature peptide
type: SequenceAnnotation
persistent identity: annotation2363643
display id: annotation2363643
version: 1
top level: BBa_K1104300
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1104300/annotation2363643/range2363643/1

https://synbiohub.org/public/igem/BBa_K1104300/annotation2363643/range2363643/1
type: Range
persistent identity: range2363643
display id: range2363643
version: 1
direction: direction/forward
top level: BBa_K1104300
start: 4
end: 105
orientation: inline

Methionine
type: SequenceAnnotation
persistent identity: annotation2363579
display id: annotation2363579
version: 1
top level: BBa_K1104300
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1104300/annotation2363579/range2363579/1

https://synbiohub.org/public/igem/BBa_K1104300/annotation2363579/range2363579/1
type: Range
persistent identity: range2363579
display id: range2363579
version: 1
direction: direction/forward
top level: BBa_K1104300
start: 1
end: 3
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

BBa_K1467103
type: ComponentDefinition
persistent identity: BBa K1467103
display id: BBa_K1467103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible PDF1.2 promotor
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:37:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PDF1.2 is a plant specific promoter and 5??? untranslated region which is induced by methyl jasmonate. The part is compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences.
mutable notes: Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences).
mutable provenance: Arabidopsis thaliana
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467103_sequence/1

BBa_E0420
type: ComponentDefinition
persistent identity: BBa E0420
display id: BBa_E0420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
creator: Caitlin Conboy
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Standard CFP output device w/o LVA tag.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0020
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_E0420_sequence/1

BBa_B0010
type: Component
persistent identity: component942787
display id: component942787
version: 1
top level: BBa_E0420
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0020
type: Component
persistent identity: component942782
display id: component942782
version: 1
top level: BBa_E0420
access: public
definition: ecfp

BBa_B0034
type: Component
persistent identity: component942780
display id: component942780
version: 1
top level: BBa_E0420
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component942797
display id: component942797
version: 1
top level: BBa_E0420
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942797
display id: annotation942797
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
type: Range
persistent identity: range942797
display id: range942797
version: 1
direction: direction/forward
top level: BBa_E0420
start: 838
end: 878
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation942782
display id: annotation942782
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
type: Range
persistent identity: range942782
display id: range942782
version: 1
direction: direction/forward
top level: BBa_E0420
start: 19
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942787
display id: annotation942787
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
type: Range
persistent identity: range942787
display id: range942787
version: 1
direction: direction/forward
top level: BBa_E0420
start: 750
end: 829
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942780
display id: annotation942780
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
type: Range
persistent identity: range942780
display id: range942780
version: 1
direction: direction/forward
top level: BBa_E0420
start: 1
end: 12
orientation: inline

BBa_K1484103
type: ComponentDefinition
persistent identity: BBa K1484103
display id: BBa_K1484103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Benzalacetone Synthase, compatible with MoClo
creator: Guy Lewy
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1864_
m user id: 0
owner id: 21057
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Synthesis of BA
mutable notes: Restriction sites
mutable provenance: Raspberry genome, lab in Kyoto
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Benzalacetone Synthase
sequence annotation: MoClo fusion site
sequence: https://synbiohub.org/public/igem/BBa_K1484103_sequence/1

Benzalacetone Synthase
type: SequenceAnnotation
persistent identity: annotation2416922
display id: annotation2416922
version: 1
top level: BBa_K1484103
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1484103/annotation2416922/range2416922/1

https://synbiohub.org/public/igem/BBa_K1484103/annotation2416922/range2416922/1
type: Range
persistent identity: range2416922
display id: range2416922
version: 1
direction: direction/forward
top level: BBa_K1484103
start: 9
end: 1163
orientation: inline

MoClo fusion site
type: SequenceAnnotation
persistent identity: annotation2416923
display id: annotation2416923
version: 1
top level: BBa_K1484103
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484103/annotation2416923/range2416923/1

https://synbiohub.org/public/igem/BBa_K1484103/annotation2416923/range2416923/1
type: Range
persistent identity: range2416923
display id: range2416923
version: 1
direction: direction/forward
top level: BBa_K1484103
start: 1
end: 11
orientation: inline

MoClo fusion site
type: SequenceAnnotation
persistent identity: annotation2416924
display id: annotation2416924
version: 1
top level: BBa_K1484103
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484103/annotation2416924/range2416924/1

https://synbiohub.org/public/igem/BBa_K1484103/annotation2416924/range2416924/1
type: Range
persistent identity: range2416924
display id: range2416924
version: 1
direction: direction/forward
top level: BBa_K1484103
start: 1164
end: 1174
orientation: inline

BBa_K1467104
type: ComponentDefinition
persistent identity: BBa K1467104
display id: BBa_K1467104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible MAS promoter
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:22:30Z
discontinued: false
dominant: false
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: MAS is a plant specific promoter which is constitutively expressed. This part is compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences.
mutable notes: Domestication for GoldenGate by removal of BsaI and BpiI recognition sequences.
mutable provenance: Agrobacterium tumefaciens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467104_sequence/1

BBa_K1114002
type: ComponentDefinition
persistent identity: BBa K1114002
display id: BBa_K1114002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23102 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23102 with EB fusion sites. See the BBa_J23102 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of BBa_J23102 with EB fusion sites. See the BBa_J23102 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114002_sequence/1

BBa_K1847008
type: ComponentDefinition
persistent identity: BBa K1847008
display id: BBa_K1847008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lldRO1-J23117-lldRO2
creator: Anna Fomitcheva Khartchenko
created: 2015-08-25T11:00:00Z
modified: 2015-09-17T08:54:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Collection of promoters regulated by LldR.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lldRO2
sequence annotation: J23117
sequence annotation: lldRO1
sequence: https://synbiohub.org/public/igem/BBa_K1847008_sequence/1

lldRO2
type: SequenceAnnotation
persistent identity: annotation2438077
display id: annotation2438077
version: 1
top level: BBa_K1847008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847008/annotation2438077/range2438077/1

https://synbiohub.org/public/igem/BBa_K1847008/annotation2438077/range2438077/1
type: Range
persistent identity: range2438077
display id: range2438077
version: 1
direction: direction/forward
top level: BBa_K1847008
start: 127
end: 143
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2438076
display id: annotation2438076
version: 1
top level: BBa_K1847008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847008/annotation2438076/range2438076/1

https://synbiohub.org/public/igem/BBa_K1847008/annotation2438076/range2438076/1
type: Range
persistent identity: range2438076
display id: range2438076
version: 1
direction: direction/forward
top level: BBa_K1847008
start: 72
end: 106
orientation: inline

lldRO1
type: SequenceAnnotation
persistent identity: annotation2438075
display id: annotation2438075
version: 1
top level: BBa_K1847008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1847008/annotation2438075/range2438075/1

https://synbiohub.org/public/igem/BBa_K1847008/annotation2438075/range2438075/1
type: Range
persistent identity: range2438075
display id: range2438075
version: 1
direction: direction/forward
top level: BBa_K1847008
start: 1
end: 17
orientation: inline

BBa_K1114009
type: ComponentDefinition
persistent identity: BBa K1114009
display id: BBa_K1114009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23106 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23106 with AB fusion sites. See the BBa_J23106 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable notes: This is the MoClo formatted part of BBa_J23106 with AB fusion sites. See the BBa_J23106 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114009_sequence/1

BBa_K1114014
type: ComponentDefinition
persistent identity: BBa K1114014
display id: BBa_K1114014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23112 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: __NOTOC__ BBa_K1114014 short BBa_K1114014 SequenceAndFeatures ===Design Notes=== This is the MoClo formatted part of BBa_J23112 with AB fusion sites. See the BBa_J23112 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: __NOTOC__ BBa_K1114014 short BBa_K1114014 SequenceAndFeatures ===Design Notes=== This is the MoClo formatted part of BBa_J23112 with AB fusion sites. See the BBa_J23112 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114014_sequence/1

BBa_K115002
type: ComponentDefinition
persistent identity: BBa K115002
display id: BBa_K115002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (FourU)
creator: Bastiaan van den Berg
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator.
mutable notes: Part of the sequence is altered because of the scar...
mutable provenance: Salmonella Enterica Tyhpy (CP000886.1)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: scar adaptation
sequence annotation: predicted stem-loop extending to one position before start codon
sequence annotation: stem_loop
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K115002_sequence/1

scar adaptation
type: SequenceAnnotation
persistent identity: annotation1966899
display id: annotation1966899
version: 1
top level: BBa_K115002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1

https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
type: Range
persistent identity: range1966899
display id: range1966899
version: 1
direction: direction/forward
top level: BBa_K115002
start: 23
end: 28
orientation: inline

predicted stem-loop extending to one position before start codon
type: SequenceAnnotation
persistent identity: annotation1966931
display id: annotation1966931
version: 1
top level: BBa_K115002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1

https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
type: Range
persistent identity: range1966931
display id: range1966931
version: 1
direction: direction/forward
top level: BBa_K115002
start: 24
end: 52
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1966930
display id: annotation1966930
version: 1
top level: BBa_K115002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1

https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
type: Range
persistent identity: range1966930
display id: range1966930
version: 1
direction: direction/forward
top level: BBa_K115002
start: 1
end: 18
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1966897
display id: annotation1966897
version: 1
top level: BBa_K115002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1

https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
type: Range
persistent identity: range1966897
display id: range1966897
version: 1
direction: direction/forward
top level: BBa_K115002
start: 47
end: 52
orientation: inline

Switch
type: ComponentDefinition
persistent identity: BBa K1185000
display id: BBa_K1185000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B.subtilis Rod to L-form Switch
creator: Vincent Leonardo, Christopher Wall and Robert Mckiernan
created: 2013-08-14T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
m user id: 0
owner id: 16794
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This BioBrick facilitates the switching of Bacillus subtilis cells between rod-shape to L-form, a cell wall-less form of bacteria. It consists of chloramphenicol acetyl-transferase (cat) gene and a xylose-controlled promoter (PxylR) which are flanked by ~300bp pbpB gene at the 5???end and ~300bp of murE gene at 3???end. These flanking sequences allows the integration of the BioBrick into the chromosome by homologous recombination replacing the region containing the end of pbpb gene and the beginning of the murE gene, which are involved in peptidoglycan biosynthesis. This event replaces the constituitive murE promoter with a PxylR upstream of the murE gene. Therefore, when xylose is present cell wall biosynthesis is switched on, giving rod cells. When xylose is not present, the cells will become L-forms. Simply transform the BioBrick into competent B. subtilis cells and through recombination it will integrate into cells chromosome. Plate out the transformants onto 1.5% lysogeny broth agar containing 5??g/ml Chloramphenicol and 0.8 %( w/v) xylose. This will give you rod cells containing biobrick. To get L-forms, inoculate some colonies into a solution containing an equal amount of nutrient broth and MSM. Also inoculate some transformants into the same media with the addition of 0.8 %( w/v) xylose to clarify that the switch BioBrick is working. Use microscopy to check the morphology of the cells in each media. L-forms will appear near spherical and can vary in size considerably.
mutable notes: There were 3 restriction sites that did not meet the RFC10 requirements, two EcoRI sites and one PstI. The first EcoRI restriction site was located 218bp into the whole BioBrick (inside pbpb region), we changed the sequence from GAATTC to GCATTC. The second restriction site was located 2287bp into the BioBrick in the spacer region between PxylR and murE, we changed it from GAATTC to GTATTC. The PstI site was located 197bp into the BioBrick in the pbpb region and was change from CTGCAG to CTTCAG.
mutable provenance: The part is the courtesy of Prof. Jeff Errington research group, and all the sequences come from the Bacillus subtilis genomic sequence. The pbpb section in the BioBrick was taken from end of the pbpb gene 1841-2151bp (309bp). Sequence acquired through microbes online VIMSS38051. The murE section was taken from the start of the murE gene 1-331bp. Sequence from microbes online VIMSS38053. The cat gene and PxylR gene were both come from the B. subtilis genome, but can also be found in the BioBrick Registry (BBa_K823020 and BBa_K143014).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BBa_K823020
sequence annotation: pbpb Homologous region
sequence annotation: murE RBS with spacers
sequence annotation: xylose-controlled promoter (PxylR)
sequence annotation: murE Homologous region
sequence: https://synbiohub.org/public/igem/BBa_K1185000_sequence/1

BBa_K823020
type: SequenceAnnotation
persistent identity: annotation2331841
display id: annotation2331841
version: 1
top level: Switch
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331841/range2331841/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331841/range2331841/1
type: Range
persistent identity: range2331841
display id: range2331841
version: 1
direction: direction/forward
top level: Switch
start: 794
end: 1444
orientation: inline

pbpb Homologous region
type: SequenceAnnotation
persistent identity: annotation2331840
display id: annotation2331840
version: 1
top level: Switch
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331840/range2331840/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331840/range2331840/1
type: Range
persistent identity: range2331840
display id: range2331840
version: 1
direction: direction/forward
top level: Switch
start: 1
end: 309
orientation: inline

murE RBS with spacers
type: SequenceAnnotation
persistent identity: annotation2331843
display id: annotation2331843
version: 1
top level: Switch
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331843/range2331843/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331843/range2331843/1
type: Range
persistent identity: range2331843
display id: range2331843
version: 1
direction: direction/forward
top level: Switch
start: 2228
end: 2292
orientation: inline

xylose-controlled promoter (PxylR)
type: SequenceAnnotation
persistent identity: annotation2331842
display id: annotation2331842
version: 1
top level: Switch
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331842/range2331842/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331842/range2331842/1
type: Range
persistent identity: range2331842
display id: range2331842
version: 1
direction: direction/forward
top level: Switch
start: 2147
end: 2227
orientation: inline

murE Homologous region
type: SequenceAnnotation
persistent identity: annotation2331844
display id: annotation2331844
version: 1
top level: Switch
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331844/range2331844/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331844/range2331844/1
type: Range
persistent identity: range2331844
display id: range2331844
version: 1
direction: direction/forward
top level: Switch
start: 2293
end: 2623
orientation: inline

BBa_K1033917
type: ComponentDefinition
persistent identity: BBa K1033917
display id: BBa_K1033917
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Galaxea fascicularis, gfasPurple, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Galaxea fascicularis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1033919
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_K1033919
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033917_sequence/1

BBa_B0034
type: Component
persistent identity: component2351855
display id: component2351855
version: 1
top level: BBa_K1033917
access: public
definition: BBa_B0034

BBa_K1033919
type: Component
persistent identity: component2351857
display id: component2351857
version: 1
top level: BBa_K1033917
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033919/1

BBa_J23110
type: Component
persistent identity: component2351853
display id: component2351853
version: 1
top level: BBa_K1033917
access: public
definition: BBa_J23110

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2351853
display id: annotation2351853
version: 1
top level: BBa_K1033917
location: https://synbiohub.org/public/igem/BBa_K1033917/annotation2351853/range2351853/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033917/annotation2351853/range2351853/1
type: Range
persistent identity: range2351853
display id: range2351853
version: 1
direction: direction/reverse
top level: BBa_K1033917
start: 1
end: 35
orientation: inline

BBa_K1033919
type: SequenceAnnotation
persistent identity: annotation2351857
display id: annotation2351857
version: 1
top level: BBa_K1033917
location: https://synbiohub.org/public/igem/BBa_K1033917/annotation2351857/range2351857/1
component: BBa_K1033919

https://synbiohub.org/public/igem/BBa_K1033917/annotation2351857/range2351857/1
type: Range
persistent identity: range2351857
display id: range2351857
version: 1
direction: direction/reverse
top level: BBa_K1033917
start: 62
end: 730
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351855
display id: annotation2351855
version: 1
top level: BBa_K1033917
location: https://synbiohub.org/public/igem/BBa_K1033917/annotation2351855/range2351855/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033917/annotation2351855/range2351855/1
type: Range
persistent identity: range2351855
display id: range2351855
version: 1
direction: direction/reverse
top level: BBa_K1033917
start: 44
end: 55
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0034
display id: BBa_E0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: AAV
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_E0034_sequence/1

AAV
type: SequenceAnnotation
persistent identity: annotation1245672
display id: annotation1245672
version: 1
top level: EYFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
type: Range
persistent identity: range1245672
display id: range1245672
version: 1
direction: direction/forward
top level: EYFP
start: 718
end: 756
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1245671
display id: annotation1245671
version: 1
top level: EYFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
type: Range
persistent identity: range1245671
display id: range1245671
version: 1
direction: direction/forward
top level: EYFP
start: 757
end: 762
orientation: inline

BBa_I746101
type: ComponentDefinition
persistent identity: BBa I746101
display id: BBa_I746101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I746100 (AIP sensor infrastructure - agrC + agrA with RBSes) + B0015 (Terminator)
creator: Lovelace Soirez
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2127
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is simply the AIP sensor infrastructure ([http://partsregistry.org/wiki/index.php/Part:BBa_I746001 I746001]) from the agr system with a standard registry terminator ([http://partsregistry.org/wiki/index.php/Part:BBa_B0015 B0015]) downstream. The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. This part contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.
mutable notes: N/A
mutable provenance: The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_I746100
sequence annotation: BBa_B0012
sequence annotation: BBa_I746100
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I746101_sequence/1

BBa_B0010
type: Component
persistent identity: component1952629
display id: component1952629
version: 1
top level: BBa_I746101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1952631
display id: component1952631
version: 1
top level: BBa_I746101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746100
type: Component
persistent identity: component1952628
display id: component1952628
version: 1
top level: BBa_I746101
access: public
definition: https://synbiohub.org/public/igem/BBa_I746100/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1952631
display id: annotation1952631
version: 1
top level: BBa_I746101
location: https://synbiohub.org/public/igem/BBa_I746101/annotation1952631/range1952631/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746101/annotation1952631/range1952631/1
type: Range
persistent identity: range1952631
display id: range1952631
version: 1
direction: direction/forward
top level: BBa_I746101
start: 2017
end: 2057
orientation: inline

BBa_I746100
type: SequenceAnnotation
persistent identity: annotation1952628
display id: annotation1952628
version: 1
top level: BBa_I746101
location: https://synbiohub.org/public/igem/BBa_I746101/annotation1952628/range1952628/1
component: BBa_I746100

https://synbiohub.org/public/igem/BBa_I746101/annotation1952628/range1952628/1
type: Range
persistent identity: range1952628
display id: range1952628
version: 1
direction: direction/reverse
top level: BBa_I746101
start: 1
end: 1920
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1952629
display id: annotation1952629
version: 1
top level: BBa_I746101
location: https://synbiohub.org/public/igem/BBa_I746101/annotation1952629/range1952629/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746101/annotation1952629/range1952629/1
type: Range
persistent identity: range1952629
display id: range1952629
version: 1
direction: direction/forward
top level: BBa_I746101
start: 1929
end: 2008
orientation: inline

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter for expression of functional RNA
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Test
mutable notes: None.
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
direction: direction/forward
top level: BBa_K1614000
start: 1
end: 17
orientation: inline

BBa_K1033931
type: ComponentDefinition
persistent identity: BBa K1033931
display id: BBa_K1033931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilGFP, yellow chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.
mutable notes: Two illegal internal restriction sites (EcoRI and PstI) were removed.
mutable provenance: Acropora millepora. The DNA was provided by Jeffrey Miller at UCLA, and was made BioBrick-compatible by removing two illegal internal restriction sites (EcoRI and PstI).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K592010
sequence: https://synbiohub.org/public/igem/BBa_K1033931_sequence/1

BBa_K592010
type: Component
persistent identity: component2351898
display id: component2351898
version: 1
top level: BBa_K1033931
access: public
definition: https://synbiohub.org/public/igem/BBa_K592010/1

BBa_B0034
type: Component
persistent identity: component2351896
display id: component2351896
version: 1
top level: BBa_K1033931
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351896
display id: annotation2351896
version: 1
top level: BBa_K1033931
location: https://synbiohub.org/public/igem/BBa_K1033931/annotation2351896/range2351896/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033931/annotation2351896/range2351896/1
type: Range
persistent identity: range2351896
display id: range2351896
version: 1
direction: direction/reverse
top level: BBa_K1033931
start: 1
end: 12
orientation: inline

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2351898
display id: annotation2351898
version: 1
top level: BBa_K1033931
location: https://synbiohub.org/public/igem/BBa_K1033931/annotation2351898/range2351898/1
component: BBa_K592010

https://synbiohub.org/public/igem/BBa_K1033931/annotation2351898/range2351898/1
type: Range
persistent identity: range2351898
display id: range2351898
version: 1
direction: direction/reverse
top level: BBa_K1033931
start: 19
end: 717
orientation: inline

BBa_K1390002
type: ComponentDefinition
persistent identity: BBa K1390002
display id: BBa_K1390002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mmoC
creator: Stefan D??bel
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:13Z
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mmoC is one subunit of the mmo from Methylococcus capsulatus bath
mutable notes: the brick is constructed in the RCF10 standard
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390002_sequence/1

BBa_K950000
type: ComponentDefinition
persistent identity: BBa K950000
display id: BBa_K950000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast fet3 promotor
creator: Jakob Matthes
created: 2012-07-09T11:00:00Z
modified: 2015-09-25T08:36:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1218_
m user id: 26366
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: promotor of the fet3 gene from S. cerevisiae, fet3 is repressed on high iron levels.
mutable notes: length of promoter was chosen to include binding sites and to obtain good primers
mutable provenance: genomic upstream sequence of fet3 gene from the Saccharomyces Genome Database (http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=fet3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: binding site
sequence: https://synbiohub.org/public/igem/BBa_K950000_sequence/1

binding site
type: SequenceAnnotation
persistent identity: annotation2182746
display id: annotation2182746
version: 1
top level: BBa_K950000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950000/annotation2182746/range2182746/1

https://synbiohub.org/public/igem/BBa_K950000/annotation2182746/range2182746/1
type: Range
persistent identity: range2182746
display id: range2182746
version: 1
direction: direction/forward
top level: BBa_K950000
start: 264
end: 268
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL-making Enzyme, luxI (+RBS)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0034.C0061
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
access: public
definition: luxI

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
access: public
definition: BBa_B0034

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
direction: direction/forward
top level: BBa_C0261
start: 19
end: 636
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
direction: direction/forward
top level: BBa_C0261
start: 1
end: 12
orientation: inline

BBa_K1073002
type: ComponentDefinition
persistent identity: BBa K1073002
display id: BBa_K1073002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible promoter of the lux quorum sensing system with RBS
creator: iGEM Team Braunschweig 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is derived from BBa_R0062 and BBa_B0032. It combines the lux induced promoter with an RBS and can be used to clone it in front of a desired gene of interest, which shall be induced by lux.
mutable notes: no considerations
mutable provenance: The construct was combined from BBa_R0062 and BBa_B0032, which were taken from the 2013 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0032
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1073002_sequence/1

BBa_B0032
type: Component
persistent identity: component2346222
display id: component2346222
version: 1
top level: BBa_K1073002
access: public
definition: BBa_B0032

BBa_R0062
type: Component
persistent identity: component2346216
display id: component2346216
version: 1
top level: BBa_K1073002
access: public
definition: lux pR

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2346216
display id: annotation2346216
version: 1
top level: BBa_K1073002
location: https://synbiohub.org/public/igem/BBa_K1073002/annotation2346216/range2346216/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1073002/annotation2346216/range2346216/1
type: Range
persistent identity: range2346216
display id: range2346216
version: 1
direction: direction/forward
top level: BBa_K1073002
start: 1
end: 55
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2346222
display id: annotation2346222
version: 1
top level: BBa_K1073002
location: https://synbiohub.org/public/igem/BBa_K1073002/annotation2346222/range2346222/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073002/annotation2346222/range2346222/1
type: Range
persistent identity: range2346222
display id: range2346222
version: 1
direction: direction/forward
top level: BBa_K1073002
start: 64
end: 76
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: SsrA
sequence annotation: 2
sequence annotation: A (Q->L)
sequence annotation: BBa_E0032
sequence annotation: YFP (LVA)
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
direction: direction/forward
top level: YFP
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
direction: direction/forward
top level: YFP
start: 757
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
direction: direction/forward
top level: YFP
start: 242
end: 242
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxICDABEG (+/-)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
direction: direction/forward
top level: BBa_B0011
start: 13
end: 35
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
direction: direction/forward
top level: BBa_B0011
start: 1
end: 46
orientation: inline

BBa_J23113
type: ComponentDefinition
persistent identity: BBa J23113
display id: BBa_J23113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23113_sequence/1

BBa_K1150017
type: ComponentDefinition
persistent identity: BBa K1150017
display id: BBa_K1150017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9 with CMV promoter
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ..
mutable notes: ..
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1150012
component: BBa_K1150010
component: BBa_K1150016
component: BBa_K1150000
component: BBa_K747096
sequence annotation: BBa_K1150016
sequence annotation: BBa_K1150012
sequence annotation: BBa_K1150010
sequence annotation: BBa_K1150000
sequence annotation: BBa_K747096
sequence: https://synbiohub.org/public/igem/BBa_K1150017_sequence/1

BBa_K1150012
type: Component
persistent identity: component2354373
display id: component2354373
version: 1
top level: BBa_K1150017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1150012/1

BBa_K1150010
type: Component
persistent identity: component2354371
display id: component2354371
version: 1
top level: BBa_K1150017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1150010/1

BBa_K1150016
type: Component
persistent identity: component2354365
display id: component2354365
version: 1
top level: BBa_K1150017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1150016/1

BBa_K1150000
type: Component
persistent identity: component2354369
display id: component2354369
version: 1
top level: BBa_K1150017
access: public
definition: BBa_K1150000

BBa_K1150010
type: Component
persistent identity: component2354367
display id: component2354367
version: 1
top level: BBa_K1150017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1150010/1

BBa_K747096
type: Component
persistent identity: component2354363
display id: component2354363
version: 1
top level: BBa_K1150017
access: public
definition: https://synbiohub.org/public/igem/BBa_K747096/1

BBa_K1150016
type: SequenceAnnotation
persistent identity: annotation2354365
display id: annotation2354365
version: 1
top level: BBa_K1150017
location: https://synbiohub.org/public/igem/BBa_K1150017/annotation2354365/range2354365/1
component: BBa_K1150016

https://synbiohub.org/public/igem/BBa_K1150017/annotation2354365/range2354365/1
type: Range
persistent identity: range2354365
display id: range2354365
version: 1
direction: direction/reverse
top level: BBa_K1150017
start: 589
end: 615
orientation: inline

BBa_K1150012
type: SequenceAnnotation
persistent identity: annotation2354373
display id: annotation2354373
version: 1
top level: BBa_K1150017
location: https://synbiohub.org/public/igem/BBa_K1150017/annotation2354373/range2354373/1
component: BBa_K1150012

https://synbiohub.org/public/igem/BBa_K1150017/annotation2354373/range2354373/1
type: Range
persistent identity: range2354373
display id: range2354373
version: 1
direction: direction/reverse
top level: BBa_K1150017
start: 4791
end: 5012
orientation: inline

BBa_K1150010
type: SequenceAnnotation
persistent identity: annotation2354367
display id: annotation2354367
version: 1
top level: BBa_K1150017
location: https://synbiohub.org/public/igem/BBa_K1150017/annotation2354367/range2354367/1
component: BBa_K1150010

https://synbiohub.org/public/igem/BBa_K1150017/annotation2354367/range2354367/1
type: Range
persistent identity: range2354367
display id: range2354367
version: 1
direction: direction/reverse
top level: BBa_K1150017
start: 624
end: 644
orientation: inline

BBa_K1150010
type: SequenceAnnotation
persistent identity: annotation2354371
display id: annotation2354371
version: 1
top level: BBa_K1150017
location: https://synbiohub.org/public/igem/BBa_K1150017/annotation2354371/range2354371/1
component: BBa_K1150010

https://synbiohub.org/public/igem/BBa_K1150017/annotation2354371/range2354371/1
type: Range
persistent identity: range2354371
display id: range2354371
version: 1
direction: direction/reverse
top level: BBa_K1150017
start: 4762
end: 4782
orientation: inline

BBa_K1150000
type: SequenceAnnotation
persistent identity: annotation2354369
display id: annotation2354369
version: 1
top level: BBa_K1150017
location: https://synbiohub.org/public/igem/BBa_K1150017/annotation2354369/range2354369/1
component: BBa_K1150000

https://synbiohub.org/public/igem/BBa_K1150017/annotation2354369/range2354369/1
type: Range
persistent identity: range2354369
display id: range2354369
version: 1
direction: direction/reverse
top level: BBa_K1150017
start: 653
end: 4753
orientation: inline

BBa_K747096
type: SequenceAnnotation
persistent identity: annotation2354363
display id: annotation2354363
version: 1
top level: BBa_K1150017
location: https://synbiohub.org/public/igem/BBa_K1150017/annotation2354363/range2354363/1
component: BBa_K747096

https://synbiohub.org/public/igem/BBa_K1150017/annotation2354363/range2354363/1
type: Range
persistent identity: range2354363
display id: range2354363
version: 1
direction: direction/reverse
top level: BBa_K1150017
start: 1
end: 580
orientation: inline

BBa_P0312
type: ComponentDefinition
persistent identity: BBa P0312
display id: BBa_P0312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> LacI [S0156]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0012
component: BBa_B0012
component: BBa_B0010
component: BBa_B0033
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0012
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_P0312_sequence/1

BBa_C0012
type: Component
persistent identity: component944617
display id: component944617
version: 1
top level: BBa_P0312
access: public
definition: lacI

BBa_B0012
type: Component
persistent identity: component944634
display id: component944634
version: 1
top level: BBa_P0312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944624
display id: component944624
version: 1
top level: BBa_P0312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0033
type: Component
persistent identity: component944606
display id: component944606
version: 1
top level: BBa_P0312
access: public
definition: BBa_B0033

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944634
display id: annotation944634
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944634/range944634/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0312/annotation944634/range944634/1
type: Range
persistent identity: range944634
display id: range944634
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1267
end: 1307
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944624
display id: annotation944624
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944624/range944624/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0312/annotation944624/range944624/1
type: Range
persistent identity: range944624
display id: range944624
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1179
end: 1258
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation944617
display id: annotation944617
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944617/range944617/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_P0312/annotation944617/range944617/1
type: Range
persistent identity: range944617
display id: range944617
version: 1
direction: direction/forward
top level: BBa_P0312
start: 18
end: 1145
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944606
display id: annotation944606
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944606/range944606/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0312/annotation944606/range944606/1
type: Range
persistent identity: range944606
display id: range944606
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1
end: 11
orientation: inline

BBa_K1033930
type: ComponentDefinition
persistent identity: BBa K1033930
display id: BBa_K1033930
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP, blue/purple chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation.
mutable notes: Illegal internal restriction site had to be removed (EcoRI).
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K592009
sequence annotation: BBa_K592009
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033930_sequence/1

BBa_B0034
type: Component
persistent identity: component2351892
display id: component2351892
version: 1
top level: BBa_K1033930
access: public
definition: BBa_B0034

BBa_K592009
type: Component
persistent identity: component2351894
display id: component2351894
version: 1
top level: BBa_K1033930
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2351894
display id: annotation2351894
version: 1
top level: BBa_K1033930
location: https://synbiohub.org/public/igem/BBa_K1033930/annotation2351894/range2351894/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1033930/annotation2351894/range2351894/1
type: Range
persistent identity: range2351894
display id: range2351894
version: 1
direction: direction/reverse
top level: BBa_K1033930
start: 19
end: 687
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351892
display id: annotation2351892
version: 1
top level: BBa_K1033930
location: https://synbiohub.org/public/igem/BBa_K1033930/annotation2351892/range2351892/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033930/annotation2351892/range2351892/1
type: Range
persistent identity: range2351892
display id: range2351892
version: 1
direction: direction/reverse
top level: BBa_K1033930
start: 1
end: 12
orientation: inline

BBa_K1742002
type: ComponentDefinition
persistent identity: BBa K1742002
display id: BBa_K1742002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145N
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:27:00Z
discontinued: false
dominant: false
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn.
mutable notes: It is codon optimized for plants
mutable provenance: Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742002_sequence/1

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: cI 434
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
direction: direction/forward
top level: cI 434
start: 631
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
direction: direction/forward
top level: cI 434
start: 670
end: 694
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

nirS
type: ComponentDefinition
persistent identity: BBa K1356003
display id: BBa_K1356003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitrite Reductase (nirS) from Pseudomonas aeruginosa PAO1
creator: Cameron Sargent
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1732_
m user id: 0
owner id: 20075
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This gene codes for the nitrite reductase (nirS) that converts nitrite (NO2-) into nitric oxide (NO). It is cloned into the pSB1C3
mutable notes: Nothing
mutable provenance: P. aeruginosa PAO1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1356003_sequence/1

BBa_K1114011
type: ComponentDefinition
persistent identity: BBa K1114011
display id: BBa_K1114011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23108 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K1114011 BBa_K1114011 SequenceAndFeatures This is the MoClo formatted part of BBa_J23108 with AB fusion sites. See the BBa_J23108 page for full information on the part. This is a MoClo level 0 promoter with a flanking site A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: BBa_K1114011 BBa_K1114011 SequenceAndFeatures This is the MoClo formatted part of BBa_J23108 with AB fusion sites. See the BBa_J23108 page for full information on the part. This is a MoClo level 0 promoter with a flanking site A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114011_sequence/1

BBa_K1017726
type: ComponentDefinition
persistent identity: BBa K1017726
display id: BBa_K1017726
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0030+pcya+B0032+ho1+B0030
creator: Wen-Hui Cheng
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1323_
m user id: 0
owner id: 17894
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: enzyme that can change heme into PCB
mutable notes: N.A.
mutable provenance: assemble parts form iGEM distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I15008
component: BBa_J23101
component: BBa_B0030
component: BBa_I15009
component: BBa_B0032
sequence annotation: BBa_B0030
sequence annotation: BBa_I15008
sequence annotation: BBa_I15009
sequence annotation: BBa_B0032
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1017726_sequence/1

BBa_I15008
type: Component
persistent identity: component2352259
display id: component2352259
version: 1
top level: BBa_K1017726
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_J23101
type: Component
persistent identity: component2352248
display id: component2352248
version: 1
top level: BBa_K1017726
access: public
definition: BBa_J23101

BBa_B0030
type: Component
persistent identity: component2352250
display id: component2352250
version: 1
top level: BBa_K1017726
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I15009
type: Component
persistent identity: component2352254
display id: component2352254
version: 1
top level: BBa_K1017726
access: public
definition: https://synbiohub.org/public/igem/BBa_I15009/1

BBa_B0032
type: Component
persistent identity: component2352256
display id: component2352256
version: 1
top level: BBa_K1017726
access: public
definition: BBa_B0032

BBa_B0030
type: Component
persistent identity: component2352261
display id: component2352261
version: 1
top level: BBa_K1017726
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2352261
display id: annotation2352261
version: 1
top level: BBa_K1017726
location: https://synbiohub.org/public/igem/BBa_K1017726/annotation2352261/range2352261/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1017726/annotation2352261/range2352261/1
type: Range
persistent identity: range2352261
display id: range2352261
version: 1
direction: direction/forward
top level: BBa_K1017726
start: 1626
end: 1640
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2352259
display id: annotation2352259
version: 1
top level: BBa_K1017726
location: https://synbiohub.org/public/igem/BBa_K1017726/annotation2352259/range2352259/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K1017726/annotation2352259/range2352259/1
type: Range
persistent identity: range2352259
display id: range2352259
version: 1
direction: direction/reverse
top level: BBa_K1017726
start: 867
end: 1617
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2352254
display id: annotation2352254
version: 1
top level: BBa_K1017726
location: https://synbiohub.org/public/igem/BBa_K1017726/annotation2352254/range2352254/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K1017726/annotation2352254/range2352254/1
type: Range
persistent identity: range2352254
display id: range2352254
version: 1
direction: direction/reverse
top level: BBa_K1017726
start: 65
end: 839
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2352250
display id: annotation2352250
version: 1
top level: BBa_K1017726
location: https://synbiohub.org/public/igem/BBa_K1017726/annotation2352250/range2352250/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1017726/annotation2352250/range2352250/1
type: Range
persistent identity: range2352250
display id: range2352250
version: 1
direction: direction/forward
top level: BBa_K1017726
start: 44
end: 58
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2352256
display id: annotation2352256
version: 1
top level: BBa_K1017726
location: https://synbiohub.org/public/igem/BBa_K1017726/annotation2352256/range2352256/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1017726/annotation2352256/range2352256/1
type: Range
persistent identity: range2352256
display id: range2352256
version: 1
direction: direction/forward
top level: BBa_K1017726
start: 848
end: 860
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2352248
display id: annotation2352248
version: 1
top level: BBa_K1017726
location: https://synbiohub.org/public/igem/BBa_K1017726/annotation2352248/range2352248/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1017726/annotation2352248/range2352248/1
type: Range
persistent identity: range2352248
display id: range2352248
version: 1
direction: direction/reverse
top level: BBa_K1017726
start: 1
end: 35
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: BBa_R0051
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

BBa_K1159102
type: ComponentDefinition
persistent identity: BBa K1159102
display id: BBa_K1159102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: C-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary N-terminal half can be found under BBa_K1159101. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
mutable notes: x
mutable provenance: Tabacco Etch Virus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C-terminal half of TEV Protease
sequence annotation: Ser --> Val for autolysis resistance
sequence: https://synbiohub.org/public/igem/BBa_K1159102_sequence/1

C-terminal half of TEV Protease
type: SequenceAnnotation
persistent identity: annotation2341474
display id: annotation2341474
version: 1
top level: BBa_K1159102
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159102/annotation2341474/range2341474/1

https://synbiohub.org/public/igem/BBa_K1159102/annotation2341474/range2341474/1
type: Range
persistent identity: range2341474
display id: range2341474
version: 1
direction: direction/forward
top level: BBa_K1159102
start: 1
end: 354
orientation: inline

Ser --> Val for autolysis resistance
type: SequenceAnnotation
persistent identity: annotation2341475
display id: annotation2341475
version: 1
top level: BBa_K1159102
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1159102/annotation2341475/range2341475/1

https://synbiohub.org/public/igem/BBa_K1159102/annotation2341475/range2341475/1
type: Range
persistent identity: range2341475
display id: range2341475
version: 1
direction: direction/forward
top level: BBa_K1159102
start: 301
end: 303
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15016
display id: BBa_I15016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.ECFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to produce ECFP under the medium RBS B0032
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0020
component: BBa_B0032
sequence annotation: BBa_E0020
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I15016_sequence/1

BBa_E0020
type: Component
persistent identity: component1478408
display id: component1478408
version: 1
top level: ecfp
access: public
definition: ecfp

BBa_B0032
type: Component
persistent identity: component1478405
display id: component1478405
version: 1
top level: ecfp
access: public
definition: BBa_B0032

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1478408
display id: annotation1478408
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
type: Range
persistent identity: range1478408
display id: range1478408
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478405
display id: annotation1478405
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
type: Range
persistent identity: range1478405
display id: range1478405
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

BBa_K1114104
type: ComponentDefinition
persistent identity: BBa K1114104
display id: BBa_K1114104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo formatted version of B0034 with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_B0034 with BC fusion sites. See the BBa_B0034 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of BBa_B0034 with BC fusion sites. See the BBa_B0034 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114104_sequence/1

rbs+LIMS1
type: ComponentDefinition
persistent identity: BBa I742111
display id: BBa_I742111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Limonene synthase gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Ribosome binding site attached to Limonene synthase gene. Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (intermediate in mevlonate pathway). (+) Limonene is a component of lemon scent.
mutable notes: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG. LIMS cDNA was used as genomic DNA contains introns.
mutable provenance: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002) Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990) Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742110
component: BBa_J15001
sequence annotation: BBa_I742110
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_I742111_sequence/1

BBa_I742110
type: Component
persistent identity: component1955374
display id: component1955374
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_I742110/1

BBa_J15001
type: Component
persistent identity: component1955370
display id: component1955370
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1955374
display id: annotation1955374
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
component: BBa_I742110

https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
type: Range
persistent identity: range1955374
display id: range1955374
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 17
end: 1687
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1955370
display id: annotation1955370
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
type: Range
persistent identity: range1955370
display id: range1955370
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 1
end: 10
orientation: inline

BBa_K823015
type: ComponentDefinition
persistent identity: BBa K823015
display id: BBa_K823015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pxyl, a xylose inducible promoter + its regulator XylR
creator: Jara Radeck
created: 2012-09-09T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pxyl is a promoter which is repressed by XylR. In presence of Xylose, XylR leaves the operator sequence and the Pxyl is active. XylR is present in most B. subtilis strains, so this would be a second copy of the gene. XylR is provided with constittive promoter, RBS and terminator.
mutable notes: Inducible promoter, with its regulator.
mutable provenance: PCR with pXT as a template.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: XylR-operator
sequence annotation: -10 extended
sequence annotation: xylR
sequence: https://synbiohub.org/public/igem/BBa_K823015_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2182453
display id: annotation2182453
version: 1
top level: BBa_K823015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823015/annotation2182453/range2182453/1

https://synbiohub.org/public/igem/BBa_K823015/annotation2182453/range2182453/1
type: Range
persistent identity: range2182453
display id: range2182453
version: 1
direction: direction/forward
top level: BBa_K823015
start: 1433
end: 1438
orientation: inline

XylR-operator
type: SequenceAnnotation
persistent identity: annotation2200462
display id: annotation2200462
version: 1
top level: BBa_K823015
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K823015/annotation2200462/range2200462/1

https://synbiohub.org/public/igem/BBa_K823015/annotation2200462/range2200462/1
type: Range
persistent identity: range2200462
display id: range2200462
version: 1
direction: direction/forward
top level: BBa_K823015
start: 1474
end: 1496
orientation: inline

-10 extended
type: SequenceAnnotation
persistent identity: annotation2200460
display id: annotation2200460
version: 1
top level: BBa_K823015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823015/annotation2200460/range2200460/1

https://synbiohub.org/public/igem/BBa_K823015/annotation2200460/range2200460/1
type: Range
persistent identity: range2200460
display id: range2200460
version: 1
direction: direction/forward
top level: BBa_K823015
start: 1451
end: 1458
orientation: inline

xylR
type: SequenceAnnotation
persistent identity: annotation2200461
display id: annotation2200461
version: 1
top level: BBa_K823015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823015/annotation2200461/range2200461/1

https://synbiohub.org/public/igem/BBa_K823015/annotation2200461/range2200461/1
type: Range
persistent identity: range2200461
display id: range2200461
version: 1
direction: direction/reverse
top level: BBa_K823015
start: 173
end: 1224
orientation: inline

BBa_I13600
type: ComponentDefinition
persistent identity: BBa I13600
display id: BBa_I13600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet with CFP reporter (without LVA tag)
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_R0040
component: BBa_B0034
component: BBa_E0020
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13600_sequence/1

BBa_B0012
type: Component
persistent identity: component947209
display id: component947209
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component947184
display id: component947184
version: 1
top level: BBa_I13600
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component947192
display id: component947192
version: 1
top level: BBa_I13600
access: public
definition: BBa_B0034

BBa_E0020
type: Component
persistent identity: component947194
display id: component947194
version: 1
top level: BBa_I13600
access: public
definition: ecfp

BBa_B0010
type: Component
persistent identity: component947199
display id: component947199
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947199
display id: annotation947199
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
type: Range
persistent identity: range947199
display id: range947199
version: 1
direction: direction/forward
top level: BBa_I13600
start: 812
end: 891
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation947194
display id: annotation947194
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
type: Range
persistent identity: range947194
display id: range947194
version: 1
direction: direction/forward
top level: BBa_I13600
start: 81
end: 803
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947184
display id: annotation947184
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
type: Range
persistent identity: range947184
display id: range947184
version: 1
direction: direction/forward
top level: BBa_I13600
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947209
display id: annotation947209
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
type: Range
persistent identity: range947209
display id: range947209
version: 1
direction: direction/forward
top level: BBa_I13600
start: 900
end: 940
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947192
display id: annotation947192
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
type: Range
persistent identity: range947192
display id: range947192
version: 1
direction: direction/forward
top level: BBa_I13600
start: 63
end: 74
orientation: inline

BBa_K1114007
type: ComponentDefinition
persistent identity: BBa K1114007
display id: BBa_K1114007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23105 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_J23105 with AB fusion sites. See the BBa_J23105 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable notes: This is the MoClo formatted part of BBa_J23105 with AB fusion sites. See the BBa_J23105 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114007_sequence/1

BBa_I746200
type: ComponentDefinition
persistent identity: BBa I746200
display id: BBa_I746200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
creator: Narin Hengrung
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2118
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted.
mutable notes: The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method.
mutable provenance: The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I746200_sequence/1

BBa_K325101
type: ComponentDefinition
persistent identity: BBa K325101
display id: BBa_K325101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EPIC Firefly Luciferase Photinus Pyralis (E. coli optimised)
creator: Ben Reeve and Hannah Copley
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: EPIC luciferase (Photinus pyralis)
sequence annotation: stop
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K325101_sequence/1

EPIC luciferase (Photinus pyralis)
type: SequenceAnnotation
persistent identity: annotation2097938
display id: annotation2097938
version: 1
top level: BBa_K325101
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325101/annotation2097938/range2097938/1

https://synbiohub.org/public/igem/BBa_K325101/annotation2097938/range2097938/1
type: Range
persistent identity: range2097938
display id: range2097938
version: 1
direction: direction/forward
top level: BBa_K325101
start: 18
end: 1667
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2097944
display id: annotation2097944
version: 1
top level: BBa_K325101
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325101/annotation2097944/range2097944/1

https://synbiohub.org/public/igem/BBa_K325101/annotation2097944/range2097944/1
type: Range
persistent identity: range2097944
display id: range2097944
version: 1
direction: direction/forward
top level: BBa_K325101
start: 1668
end: 1673
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2097943
display id: annotation2097943
version: 1
top level: BBa_K325101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325101/annotation2097943/range2097943/1

https://synbiohub.org/public/igem/BBa_K325101/annotation2097943/range2097943/1
type: Range
persistent identity: range2097943
display id: range2097943
version: 1
direction: direction/forward
top level: BBa_K325101
start: 1
end: 17
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

REACh2
type: ComponentDefinition
persistent identity: BBa K1319002
display id: BBa_K1319002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFC[25]-compatible dark quencher based on K1319000 (E0030)
creator: Michael Osthege, Florian Gohr
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP). Three mutations were introduced that eliminated fluorescence: * L90I * Y145W * H148R
mutable notes: Point mutations were introduced, starting from K1319000 (RFC25-YFP) as the template.
mutable provenance: The part is derived from K1319000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: REACh2
sequence: https://synbiohub.org/public/igem/BBa_K1319002_sequence/1

REACh2
type: SequenceAnnotation
persistent identity: annotation2390763
display id: annotation2390763
version: 1
top level: REACh2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1319002/annotation2390763/range2390763/1

https://synbiohub.org/public/igem/BBa_K1319002/annotation2390763/range2390763/1
type: Range
persistent identity: range2390763
display id: range2390763
version: 1
direction: direction/forward
top level: REACh2
start: 1
end: 711
orientation: inline

BBa_K1333005
type: ComponentDefinition
persistent identity: BBa K1333005
display id: BBa_K1333005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13 bacteriophage g8p with a FLAG tag on C-terminal
creator: Huang Junxiang, Wang Xizi
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: pVIII with a FLAG tag on C-terminal
mutable notes: pVIII with a FLAG tag on C-terminal
mutable provenance: pVIII with a FLAG tag on C-terminal
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FLAG
sequence annotation: g8p
sequence: https://synbiohub.org/public/igem/BBa_K1333005_sequence/1

FLAG
type: SequenceAnnotation
persistent identity: annotation2386089
display id: annotation2386089
version: 1
top level: BBa_K1333005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1333005/annotation2386089/range2386089/1

https://synbiohub.org/public/igem/BBa_K1333005/annotation2386089/range2386089/1
type: Range
persistent identity: range2386089
display id: range2386089
version: 1
direction: direction/forward
top level: BBa_K1333005
start: 229
end: 255
orientation: inline

g8p
type: SequenceAnnotation
persistent identity: annotation2386088
display id: annotation2386088
version: 1
top level: BBa_K1333005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1333005/annotation2386088/range2386088/1

https://synbiohub.org/public/igem/BBa_K1333005/annotation2386088/range2386088/1
type: Range
persistent identity: range2386088
display id: range2386088
version: 1
direction: direction/forward
top level: BBa_K1333005
start: 1
end: 219
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pL)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
mutable provenance: V. fischeri.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: BBa_R0063
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: Putative LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
direction: direction/forward
top level: lux pL
start: 128
end: 128
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 151
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
direction: direction/forward
top level: lux pL
start: 115
end: 122
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
direction: direction/forward
top level: lux pL
start: 89
end: 94
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
direction: direction/forward
top level: lux pL
start: 130
end: 149
orientation: inline

BBa_K1114107
type: ComponentDefinition
persistent identity: BBa K1114107
display id: BBa_K1114107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic Design RBS 2, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BCD2
sequence annotation: MoClo Fusion Site B
sequence annotation: MoClo Fusion Site C
sequence: https://synbiohub.org/public/igem/BBa_K1114107_sequence/1

BCD2
type: SequenceAnnotation
persistent identity: annotation2361724
display id: annotation2361724
version: 1
top level: BBa_K1114107
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361724/range2361724/1

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361724/range2361724/1
type: Range
persistent identity: range2361724
display id: range2361724
version: 1
direction: direction/forward
top level: BBa_K1114107
start: 5
end: 88
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361727
display id: annotation2361727
version: 1
top level: BBa_K1114107
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361727/range2361727/1

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361727/range2361727/1
type: Range
persistent identity: range2361727
display id: range2361727
version: 1
direction: direction/forward
top level: BBa_K1114107
start: 1
end: 4
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361728
display id: annotation2361728
version: 1
top level: BBa_K1114107
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361728/range2361728/1

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361728/range2361728/1
type: Range
persistent identity: range2361728
display id: range2361728
version: 1
direction: direction/forward
top level: BBa_K1114107
start: 89
end: 92
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0026
display id: BBa_E0026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0026_sequence/1

BBa_K801011
type: ComponentDefinition
persistent identity: BBa K801011
display id: BBa_K801011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF1 yeast terminator
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TEF1 terminator of ''S. cervisiae''.
mutable notes: Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: TEF1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801011_sequence/1

TEF1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202142
display id: annotation2202142
version: 1
top level: BBa_K801011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1

https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1
type: Range
persistent identity: range2202142
display id: range2202142
version: 1
direction: direction/forward
top level: BBa_K801011
start: 1
end: 507
orientation: inline

BBa_K311004
type: ComponentDefinition
persistent identity: BBa K311004
display id: BBa_K311004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complement of Ethanolamine Utilization Microcompartment proteins from Salmonella LT2
creator: Matthew Adams, Swati Choudhary, Anthony Goering, Rachel Farr, Ethan Johnson, Annie Kathuria, Poonam Srivastava, Ian Windsor
created: 2010-10-14T11:00:00Z
modified: 2015-05-08T01:11:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _430_
m user id: 0
owner id: 7095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Three proteins subunits of a bacterial micro-compartment. The genes are in the pSB1C3 plasmid. A total of 5 micro-compartment genes are cloned in a single plasmid. They are arranged as two sets of two genes (in a single operon) with one gene which is upstream to the pairs. They are flanked directly upstream by a mutated Lac promoter to make them constitutively expressed. The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes.
mutable notes: The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes. The genes were ligated into a modified pUC biobrick vector containing a constitutive promoter. The genes were taking in their native operons and ligated sequential according to size.
mutable provenance: Salmonella enterica LT2 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: EutK
sequence annotation: modified plac
sequence annotation: EutL
sequence annotation: EutN
sequence annotation: EutM
sequence annotation: Eut S
sequence annotation: BBa_K311004
sequence: https://synbiohub.org/public/igem/BBa_K311004_sequence/1

EutK
type: SequenceAnnotation
persistent identity: annotation2099766
display id: annotation2099766
version: 1
top level: BBa_K311004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099766/range2099766/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099766/range2099766/1
type: Range
persistent identity: range2099766
display id: range2099766
version: 1
direction: direction/forward
top level: BBa_K311004
start: 2277
end: 2771
orientation: inline

modified plac
type: SequenceAnnotation
persistent identity: annotation2099764
display id: annotation2099764
version: 1
top level: BBa_K311004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099764/range2099764/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099764/range2099764/1
type: Range
persistent identity: range2099764
display id: range2099764
version: 1
direction: direction/forward
top level: BBa_K311004
start: 1466
end: 1589
orientation: inline

modified plac
type: SequenceAnnotation
persistent identity: annotation2099761
display id: annotation2099761
version: 1
top level: BBa_K311004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099761/range2099761/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099761/range2099761/1
type: Range
persistent identity: range2099761
display id: range2099761
version: 1
direction: direction/forward
top level: BBa_K311004
start: 556
end: 679
orientation: inline

EutL
type: SequenceAnnotation
persistent identity: annotation2099765
display id: annotation2099765
version: 1
top level: BBa_K311004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099765/range2099765/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099765/range2099765/1
type: Range
persistent identity: range2099765
display id: range2099765
version: 1
direction: direction/forward
top level: BBa_K311004
start: 1605
end: 2264
orientation: inline

EutN
type: SequenceAnnotation
persistent identity: annotation2099763
display id: annotation2099763
version: 1
top level: BBa_K311004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099763/range2099763/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099763/range2099763/1
type: Range
persistent identity: range2099763
display id: range2099763
version: 1
direction: direction/forward
top level: BBa_K311004
start: 1086
end: 1385
orientation: inline

EutM
type: SequenceAnnotation
persistent identity: annotation2099762
display id: annotation2099762
version: 1
top level: BBa_K311004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099762/range2099762/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099762/range2099762/1
type: Range
persistent identity: range2099762
display id: range2099762
version: 1
direction: direction/forward
top level: BBa_K311004
start: 695
end: 985
orientation: inline

Eut S
type: SequenceAnnotation
persistent identity: annotation2099760
display id: annotation2099760
version: 1
top level: BBa_K311004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099760/range2099760/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099760/range2099760/1
type: Range
persistent identity: range2099760
display id: range2099760
version: 1
direction: direction/forward
top level: BBa_K311004
start: 140
end: 475
orientation: inline

modified plac
type: SequenceAnnotation
persistent identity: annotation2099759
display id: annotation2099759
version: 1
top level: BBa_K311004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099759/range2099759/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099759/range2099759/1
type: Range
persistent identity: range2099759
display id: range2099759
version: 1
direction: direction/forward
top level: BBa_K311004
start: 1
end: 124
orientation: inline

BBa_K311004
type: SequenceAnnotation
persistent identity: annotation2099767
display id: annotation2099767
version: 1
top level: BBa_K311004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K311004/annotation2099767/range2099767/1

https://synbiohub.org/public/igem/BBa_K311004/annotation2099767/range2099767/1
type: Range
persistent identity: range2099767
display id: range2099767
version: 1
direction: direction/reverse
top level: BBa_K311004
start: 1
end: 2771
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: SsrA
sequence annotation: CFP (LVA)
sequence annotation: BBa_E0022
sequence annotation: A
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
direction: direction/forward
top level: ECFP
start: 719
end: 756
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
direction: direction/forward
top level: ECFP
start: 69
end: 69
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
direction: direction/forward
top level: ECFP
start: 757
end: 762
orientation: inline

BBa_I0466
type: ComponentDefinition
persistent identity: BBa I0466
display id: BBa_I0466
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR Protein Generator
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR can sense N-butyryl-HSL in the media and can activate transcription from R0071. It can be used as part of a receiver device.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_C0071
sequence annotation: BBa_B0010
sequence annotation: BBa_C0071
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0466_sequence/1

BBa_B0012
type: Component
persistent identity: component943330
display id: component943330
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component943304
display id: component943304
version: 1
top level: BBa_I0466
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component943320
display id: component943320
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0071
type: Component
persistent identity: component943314
display id: component943314
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_C0071/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943320
display id: annotation943320
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
type: Range
persistent identity: range943320
display id: range943320
version: 1
direction: direction/forward
top level: BBa_I0466
start: 814
end: 893
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation943314
display id: annotation943314
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
type: Range
persistent identity: range943314
display id: range943314
version: 1
direction: direction/forward
top level: BBa_I0466
start: 19
end: 780
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943304
display id: annotation943304
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
type: Range
persistent identity: range943304
display id: range943304
version: 1
direction: direction/forward
top level: BBa_I0466
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943330
display id: annotation943330
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
type: Range
persistent identity: range943330
display id: range943330
version: 1
direction: direction/forward
top level: BBa_I0466
start: 902
end: 942
orientation: inline

BBa_P0153
type: ComponentDefinition
persistent identity: BBa P0153
display id: BBa_P0153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0167]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cII from p22. Used as the input section for Quad Part Inverters Q01530.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0053
component: BBa_B0031
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_C0053
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0153_sequence/1

BBa_C0053
type: Component
persistent identity: component944580
display id: component944580
version: 1
top level: BBa_P0153
access: public
definition: c2 P22

BBa_B0031
type: Component
persistent identity: component944568
display id: component944568
version: 1
top level: BBa_P0153
access: public
definition: BBa_B0031

BBa_B0012
type: Component
persistent identity: component944598
display id: component944598
version: 1
top level: BBa_P0153
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944588
display id: component944588
version: 1
top level: BBa_P0153
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944588
display id: annotation944588
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
type: Range
persistent identity: range944588
display id: range944588
version: 1
direction: direction/forward
top level: BBa_P0153
start: 741
end: 820
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation944580
display id: annotation944580
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
type: Range
persistent identity: range944580
display id: range944580
version: 1
direction: direction/forward
top level: BBa_P0153
start: 21
end: 707
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944598
display id: annotation944598
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
type: Range
persistent identity: range944598
display id: range944598
version: 1
direction: direction/forward
top level: BBa_P0153
start: 829
end: 869
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944568
display id: annotation944568
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
type: Range
persistent identity: range944568
display id: range944568
version: 1
direction: direction/forward
top level: BBa_P0153
start: 1
end: 14
orientation: inline

BBa_K1033903
type: ComponentDefinition
persistent identity: BBa K1033903
display id: BBa_K1033903
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple, purple chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from TinselPurple vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23110
component: BBa_K1033905
sequence annotation: BBa_J23110
sequence annotation: BBa_K1033905
sequence: https://synbiohub.org/public/igem/BBa_K1033903_sequence/1

BBa_J23110
type: Component
persistent identity: component2371213
display id: component2371213
version: 1
top level: BBa_K1033903
access: public
definition: BBa_J23110

BBa_K1033905
type: Component
persistent identity: component2371217
display id: component2371217
version: 1
top level: BBa_K1033903
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033905/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2371213
display id: annotation2371213
version: 1
top level: BBa_K1033903
location: https://synbiohub.org/public/igem/BBa_K1033903/annotation2371213/range2371213/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033903/annotation2371213/range2371213/1
type: Range
persistent identity: range2371213
display id: range2371213
version: 1
direction: direction/reverse
top level: BBa_K1033903
start: 1
end: 35
orientation: inline

BBa_K1033905
type: SequenceAnnotation
persistent identity: annotation2371217
display id: annotation2371217
version: 1
top level: BBa_K1033903
location: https://synbiohub.org/public/igem/BBa_K1033903/annotation2371217/range2371217/1
component: BBa_K1033905

https://synbiohub.org/public/igem/BBa_K1033903/annotation2371217/range2371217/1
type: Range
persistent identity: range2371217
display id: range2371217
version: 1
direction: direction/reverse
top level: BBa_K1033903
start: 44
end: 751
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0160
display id: BBa_C0160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme aiiA (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0060 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aiiA
sequence: https://synbiohub.org/public/igem/BBa_C0160_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1891585
display id: annotation1891585
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1

https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1
type: Range
persistent identity: range1891585
display id: range1891585
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 756
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (LasR & PAI regulated)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Binding region for LasR protein (positive regulation)
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
direction: direction/forward
top level: LasR+PAI
start: 46
end: 63
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
direction: direction/forward
top level: LasR+PAI
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
direction: direction/forward
top level: LasR+PAI
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
direction: direction/forward
top level: LasR+PAI
start: 140
end: 145
orientation: inline

BBa_K118005
type: ComponentDefinition
persistent identity: BBa K118005
display id: BBa_K118005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtI (phytoene dehydrogenase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) preceded by an E. coli synthetic ribosome binding site (rbs). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
mutable provenance: rbs: Escherichia coli JM109 genomic DNA. crtI: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118003
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118003
sequence: https://synbiohub.org/public/igem/BBa_K118005_sequence/1

BBa_K118003
type: Component
persistent identity: component1970360
display id: component1970360
version: 1
top level: BBa_K118005
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_J15001
type: Component
persistent identity: component1970357
display id: component1970357
version: 1
top level: BBa_K118005
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970357
display id: annotation1970357
version: 1
top level: BBa_K118005
location: https://synbiohub.org/public/igem/BBa_K118005/annotation1970357/range1970357/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118005/annotation1970357/range1970357/1
type: Range
persistent identity: range1970357
display id: range1970357
version: 1
direction: direction/reverse
top level: BBa_K118005
start: 1
end: 10
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation1970360
display id: annotation1970360
version: 1
top level: BBa_K118005
location: https://synbiohub.org/public/igem/BBa_K118005/annotation1970360/range1970360/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K118005/annotation1970360/range1970360/1
type: Range
persistent identity: range1970360
display id: range1970360
version: 1
direction: direction/reverse
top level: BBa_K118005
start: 17
end: 1498
orientation: inline

BBa_K1033922
type: ComponentDefinition
persistent identity: BBa K1033922
display id: BBa_K1033922
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: meffRed, red chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Montipora efflorescens, meffRed, naturally exhibits color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Montipora efflorescens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: meffRed
sequence: https://synbiohub.org/public/igem/BBa_K1033922_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351818
display id: annotation2351818
version: 1
top level: BBa_K1033922
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033922/annotation2351818/range2351818/1

https://synbiohub.org/public/igem/BBa_K1033922/annotation2351818/range2351818/1
type: Range
persistent identity: range2351818
display id: range2351818
version: 1
direction: direction/reverse
top level: BBa_K1033922
start: 1
end: 12
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2351817
display id: annotation2351817
version: 1
top level: BBa_K1033922
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1033922/annotation2351817/range2351817/1

https://synbiohub.org/public/igem/BBa_K1033922/annotation2351817/range2351817/1
type: Range
persistent identity: range2351817
display id: range2351817
version: 1
direction: direction/forward
top level: BBa_K1033922
start: 5
end: 8
orientation: inline

meffRed
type: SequenceAnnotation
persistent identity: annotation2351819
display id: annotation2351819
version: 1
top level: BBa_K1033922
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033922/annotation2351819/range2351819/1

https://synbiohub.org/public/igem/BBa_K1033922/annotation2351819/range2351819/1
type: Range
persistent identity: range2351819
display id: range2351819
version: 1
direction: direction/forward
top level: BBa_K1033922
start: 19
end: 726
orientation: inline

BBa_K783032
type: ComponentDefinition
persistent identity: BBa K783032
display id: BBa_K783032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_J23115
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_J23115. It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_J23115
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_J23115
sequence: https://synbiohub.org/public/igem/BBa_K783032_sequence/1

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2214233
display id: annotation2214233
version: 1
top level: BBa_K783032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K783032/annotation2214233/range2214233/1

https://synbiohub.org/public/igem/BBa_K783032/annotation2214233/range2214233/1
type: Range
persistent identity: range2214233
display id: range2214233
version: 1
direction: direction/reverse
top level: BBa_K783032
start: 1
end: 35
orientation: inline

BBa_K808027
type: ComponentDefinition
persistent identity: BBa K808027
display id: BBa_K808027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EstA : inactive fusion protein for bacterial surface expression
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Esterase A is a inactivated membrane bound esterase from Pseudomonas putida. Its active site is mutated so it shows no more hydrolytic activity. It can be used for cell surface display like its shown in the following paper: Taek Ho Yang, Jae Gu Pan, Yeon Soo Seo, Joon Shick Rhee Use of Pseudomonas putida EstA as an Anchoring Motif for Display of a Periplasmic Enzyme on the Surface of Escherichia coli Appl Environ Microbiol. 2004 December; 70(12): 6968???6976. doi: 10.1128/AEM.70.12.6968-6976.2004
mutable notes: This certain variant of EstA should be used for cell surface expression and displaying of almost every kind of enzyme. There is no ATG at its 5' because its an C-terminal fusion protein. If you want to use you need to design it in the following way: N - Peri plasmatic signal sequence - Protein of interest - EstA - C We did not use any kind of BioBrick Assembly due to the generation of stop codons. If you would like to fuse EstA to any kind of protein, please use SOE PCR or an alternative kind of assembly strategy.
mutable provenance: Our parts derives from an established inactive EstA mutant on pEST100 vector, which was used in the following paper: Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808027_sequence/1

BBa_I13602
type: ComponentDefinition
persistent identity: BBa I13602
display id: BBa_I13602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet operator with CFP reporter (with LVA tag) [R/Tc+]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_E0022
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13602_sequence/1

BBa_R0040
type: Component
persistent identity: component947254
display id: component947254
version: 1
top level: BBa_I13602
access: public
definition: p(tetR)

BBa_E0022
type: Component
persistent identity: component947274
display id: component947274
version: 1
top level: BBa_I13602
access: public
definition: ECFP

BBa_B0034
type: Component
persistent identity: component947262
display id: component947262
version: 1
top level: BBa_I13602
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component947282
display id: component947282
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component947292
display id: component947292
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947292
display id: annotation947292
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
type: Range
persistent identity: range947292
display id: range947292
version: 1
direction: direction/forward
top level: BBa_I13602
start: 939
end: 979
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation947274
display id: annotation947274
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
type: Range
persistent identity: range947274
display id: range947274
version: 1
direction: direction/forward
top level: BBa_I13602
start: 81
end: 842
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947262
display id: annotation947262
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
type: Range
persistent identity: range947262
display id: range947262
version: 1
direction: direction/forward
top level: BBa_I13602
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947282
display id: annotation947282
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
type: Range
persistent identity: range947282
display id: range947282
version: 1
direction: direction/forward
top level: BBa_I13602
start: 851
end: 930
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947254
display id: annotation947254
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
type: Range
persistent identity: range947254
display id: range947254
version: 1
direction: direction/forward
top level: BBa_I13602
start: 1
end: 54
orientation: inline

BBa_K1073022
type: ComponentDefinition
persistent identity: BBa K1073022
display id: BBa_K1073022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutively expressed chromoprotein eforRed
creator: iGEM Team Braunschweig 2013
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The chromoprotein eforRed is constitutively expressed and can be used for the marking of cells for easier differentiation.
mutable notes: no considerations.
mutable provenance: This part is a combination of BBa_K1073006 and BBa_K592012, which were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592012
component: BBa_K1073006
sequence annotation: BBa_K1073006
sequence annotation: BBa_K592012
sequence: https://synbiohub.org/public/igem/BBa_K1073022_sequence/1

BBa_K592012
type: Component
persistent identity: component2354079
display id: component2354079
version: 1
top level: BBa_K1073022
access: public
definition: https://synbiohub.org/public/igem/BBa_K592012/1

BBa_K1073006
type: Component
persistent identity: component2354077
display id: component2354077
version: 1
top level: BBa_K1073022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1073006/1

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354077
display id: annotation2354077
version: 1
top level: BBa_K1073022
location: https://synbiohub.org/public/igem/BBa_K1073022/annotation2354077/range2354077/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073022/annotation2354077/range2354077/1
type: Range
persistent identity: range2354077
display id: range2354077
version: 1
direction: direction/reverse
top level: BBa_K1073022
start: 1
end: 56
orientation: inline

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2354079
display id: annotation2354079
version: 1
top level: BBa_K1073022
location: https://synbiohub.org/public/igem/BBa_K1073022/annotation2354079/range2354079/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K1073022/annotation2354079/range2354079/1
type: Range
persistent identity: range2354079
display id: range2354079
version: 1
direction: direction/reverse
top level: BBa_K1073022
start: 63
end: 743
orientation: inline

BBa_I20270
type: ComponentDefinition
persistent identity: BBa I20270
display id: BBa_I20270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter MeasKit (J23151)
creator: Jason Kelly
created: 2008-02-06T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: J23108 inserted in the Promoter MeasKit
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J23151
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
component: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_J23151
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I20270_sequence/1

BBa_J23151
type: Component
persistent identity: component1961199
display id: component1961199
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_J23151/1

BBa_E0040
type: Component
persistent identity: component1961204
display id: component1961204
version: 1
top level: BBa_I20270
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component1961207
display id: component1961207
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1961205
display id: component1961205
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1961201
display id: component1961201
version: 1
top level: BBa_I20270
access: public
definition: BBa_B0032

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1961204
display id: annotation1961204
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961204/range1961204/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I20270/annotation1961204/range1961204/1
type: Range
persistent identity: range1961204
display id: range1961204
version: 1
direction: direction/reverse
top level: BBa_I20270
start: 63
end: 782
orientation: inline

BBa_J23151
type: SequenceAnnotation
persistent identity: annotation1961199
display id: annotation1961199
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961199/range1961199/1
component: BBa_J23151

https://synbiohub.org/public/igem/BBa_I20270/annotation1961199/range1961199/1
type: Range
persistent identity: range1961199
display id: range1961199
version: 1
direction: direction/reverse
top level: BBa_I20270
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961207
display id: annotation1961207
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961207/range1961207/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I20270/annotation1961207/range1961207/1
type: Range
persistent identity: range1961207
display id: range1961207
version: 1
direction: direction/forward
top level: BBa_I20270
start: 879
end: 919
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1961201
display id: annotation1961201
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961201/range1961201/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I20270/annotation1961201/range1961201/1
type: Range
persistent identity: range1961201
display id: range1961201
version: 1
direction: direction/forward
top level: BBa_I20270
start: 44
end: 56
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961205
display id: annotation1961205
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961205/range1961205/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I20270/annotation1961205/range1961205/1
type: Range
persistent identity: range1961205
display id: range1961205
version: 1
direction: direction/forward
top level: BBa_I20270
start: 791
end: 870
orientation: inline

BBa_K1321335
type: ComponentDefinition
persistent identity: BBa K1321335
display id: BBa_K1321335
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: acsCD
creator: Laura de Arroyo Garcia
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 23299
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Proteins C and D from the acs cellulose producing operon of Gluconacetobacter Xylinus
mutable notes: Both genes have RBS
mutable provenance: Protein coding sequence from genome of Gluconacetobacter Xylinus, resynthesized and codon optimised for E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: AcsC Start Codon
sequence annotation: AcsD Coding Sequence
sequence annotation: RBS - AcsD
sequence annotation: AcsC Coding Sequence
sequence annotation: Double Stop Codon
sequence annotation: RBS -AcsC
sequence: https://synbiohub.org/public/igem/BBa_K1321335_sequence/1

AcsC Start Codon
type: SequenceAnnotation
persistent identity: annotation2428800
display id: annotation2428800
version: 1
top level: BBa_K1321335
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1321335/annotation2428800/range2428800/1

https://synbiohub.org/public/igem/BBa_K1321335/annotation2428800/range2428800/1
type: Range
persistent identity: range2428800
display id: range2428800
version: 1
direction: direction/forward
top level: BBa_K1321335
start: 29
end: 31
orientation: inline

AcsD Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2428801
display id: annotation2428801
version: 1
top level: BBa_K1321335
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321335/annotation2428801/range2428801/1

https://synbiohub.org/public/igem/BBa_K1321335/annotation2428801/range2428801/1
type: Range
persistent identity: range2428801
display id: range2428801
version: 1
direction: direction/forward
top level: BBa_K1321335
start: 3978
end: 4445
orientation: inline

RBS - AcsD
type: SequenceAnnotation
persistent identity: annotation2428797
display id: annotation2428797
version: 1
top level: BBa_K1321335
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1321335/annotation2428797/range2428797/1

https://synbiohub.org/public/igem/BBa_K1321335/annotation2428797/range2428797/1
type: Range
persistent identity: range2428797
display id: range2428797
version: 1
direction: direction/forward
top level: BBa_K1321335
start: 3956
end: 3979
orientation: inline

AcsC Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2428799
display id: annotation2428799
version: 1
top level: BBa_K1321335
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321335/annotation2428799/range2428799/1

https://synbiohub.org/public/igem/BBa_K1321335/annotation2428799/range2428799/1
type: Range
persistent identity: range2428799
display id: range2428799
version: 1
direction: direction/forward
top level: BBa_K1321335
start: 29
end: 3934
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2428798
display id: annotation2428798
version: 1
top level: BBa_K1321335
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1321335/annotation2428798/range2428798/1

https://synbiohub.org/public/igem/BBa_K1321335/annotation2428798/range2428798/1
type: Range
persistent identity: range2428798
display id: range2428798
version: 1
direction: direction/forward
top level: BBa_K1321335
start: 3935
end: 3941
orientation: inline

RBS -AcsC
type: SequenceAnnotation
persistent identity: annotation2428796
display id: annotation2428796
version: 1
top level: BBa_K1321335
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1321335/annotation2428796/range2428796/1

https://synbiohub.org/public/igem/BBa_K1321335/annotation2428796/range2428796/1
type: Range
persistent identity: range2428796
display id: range2428796
version: 1
direction: direction/forward
top level: BBa_K1321335
start: 5
end: 22
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2428802
display id: annotation2428802
version: 1
top level: BBa_K1321335
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1321335/annotation2428802/range2428802/1

https://synbiohub.org/public/igem/BBa_K1321335/annotation2428802/range2428802/1
type: Range
persistent identity: range2428802
display id: range2428802
version: 1
direction: direction/forward
top level: BBa_K1321335
start: 4446
end: 4451
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 cI
sequence annotation: BBa_R0065
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR2 cI
sequence annotation: Lux Box
sequence annotation: lux p(R) start
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

dxs
type: ComponentDefinition
persistent identity: BBa K118000
display id: BBa_K118000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dxs coding sequence encoding 1-deoxyxylulose-5-phosphate synthase
creator: Chris French
created: 2008-07-08T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of dxs from Escherichia coli JM109. It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses teh first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dxs coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118000_sequence/1

dxs coding sequence
type: SequenceAnnotation
persistent identity: annotation1995699
display id: annotation1995699
version: 1
top level: dxs
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1

https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1
type: Range
persistent identity: range1995699
display id: range1995699
version: 1
direction: direction/forward
top level: dxs
start: 1
end: 1863
orientation: inline

BBa_K1033900
type: ComponentDefinition
persistent identity: BBa K1033900
display id: BBa_K1033900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: meffBlue, blue chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Montipora efflorescens, meffBlue, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Montipora efflorescens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23110
component: BBa_K1033901
sequence annotation: BBa_K1033901
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K1033900_sequence/1

BBa_J23110
type: Component
persistent identity: component2371203
display id: component2371203
version: 1
top level: BBa_K1033900
access: public
definition: BBa_J23110

BBa_K1033901
type: Component
persistent identity: component2371207
display id: component2371207
version: 1
top level: BBa_K1033900
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033901/1

BBa_K1033901
type: SequenceAnnotation
persistent identity: annotation2371207
display id: annotation2371207
version: 1
top level: BBa_K1033900
location: https://synbiohub.org/public/igem/BBa_K1033900/annotation2371207/range2371207/1
component: BBa_K1033901

https://synbiohub.org/public/igem/BBa_K1033900/annotation2371207/range2371207/1
type: Range
persistent identity: range2371207
display id: range2371207
version: 1
direction: direction/reverse
top level: BBa_K1033900
start: 44
end: 751
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2371203
display id: annotation2371203
version: 1
top level: BBa_K1033900
location: https://synbiohub.org/public/igem/BBa_K1033900/annotation2371203/range2371203/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033900/annotation2371203/range2371203/1
type: Range
persistent identity: range2371203
display id: range2371203
version: 1
direction: direction/reverse
top level: BBa_K1033900
start: 1
end: 35
orientation: inline

BBa_K1033282
type: ComponentDefinition
persistent identity: BBa K1033282
display id: BBa_K1033282
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP blue chromoprotein with strong promoter
creator: Anders Edlund, Mikael Strandgren
created: 2013-09-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1340_
m user id: 0
owner id: 17708
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Blue/purple chromoprotein (amilCP: BBa_K592009) with strong promotor (CP29). The promoter is supposed to work in E. coli as well as Lactobacillus. (Construct name: pSB1C3-CP29-B0034-amilCP)
mutable notes: Coming soon...
mutable provenance: Coming soon...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033222
component: BBa_K1033930
sequence annotation: BBa_K1033222
sequence annotation: BBa_K1033930
sequence: https://synbiohub.org/public/igem/BBa_K1033282_sequence/1

BBa_K1033222
type: Component
persistent identity: component2362556
display id: component2362556
version: 1
top level: BBa_K1033282
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033222/1

BBa_K1033930
type: Component
persistent identity: component2362561
display id: component2362561
version: 1
top level: BBa_K1033282
access: public
definition: BBa_K1033930

BBa_K1033222
type: SequenceAnnotation
persistent identity: annotation2362556
display id: annotation2362556
version: 1
top level: BBa_K1033282
location: https://synbiohub.org/public/igem/BBa_K1033282/annotation2362556/range2362556/1
component: BBa_K1033222

https://synbiohub.org/public/igem/BBa_K1033282/annotation2362556/range2362556/1
type: Range
persistent identity: range2362556
display id: range2362556
version: 1
direction: direction/reverse
top level: BBa_K1033282
start: 1
end: 59
orientation: inline

BBa_K1033930
type: SequenceAnnotation
persistent identity: annotation2362561
display id: annotation2362561
version: 1
top level: BBa_K1033282
location: https://synbiohub.org/public/igem/BBa_K1033282/annotation2362561/range2362561/1
component: BBa_K1033930

https://synbiohub.org/public/igem/BBa_K1033282/annotation2362561/range2362561/1
type: Range
persistent identity: range2362561
display id: range2362561
version: 1
direction: direction/reverse
top level: BBa_K1033282
start: 68
end: 754
orientation: inline

BBa_K1321333
type: ComponentDefinition
persistent identity: BBa K1321333
display id: BBa_K1321333
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB-AraC-pBAD
creator: Laura de Arroyo Garcia
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:41Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 21121
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream.
mutable provenance: This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PC
sequence annotation: pBAD
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_K1321333_sequence/1

PC
type: SequenceAnnotation
persistent identity: annotation2429238
display id: annotation2429238
version: 1
top level: BBa_K1321333
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429238/range2429238/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429238/range2429238/1
type: Range
persistent identity: range2429238
display id: range2429238
version: 1
direction: direction/reverse
top level: BBa_K1321333
start: 1042
end: 1115
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2429239
display id: annotation2429239
version: 1
top level: BBa_K1321333
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429239/range2429239/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429239/range2429239/1
type: Range
persistent identity: range2429239
display id: range2429239
version: 1
direction: direction/forward
top level: BBa_K1321333
start: 1116
end: 1210
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2429237
display id: annotation2429237
version: 1
top level: BBa_K1321333
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429237/range2429237/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429237/range2429237/1
type: Range
persistent identity: range2429237
display id: range2429237
version: 1
direction: direction/reverse
top level: BBa_K1321333
start: 1
end: 1041
orientation: inline

BBa_K387003
type: ComponentDefinition
persistent identity: BBa K387003
display id: BBa_K387003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PfdhF: fdhF promoter, a hypoxia inducible promoter
creator: Yupeng He
created: 2010-10-05T11:00:00Z
modified: 2015-05-08T01:12:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _499_
m user id: 0
owner id: 6355
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Hypoxia-inducible promoter
mutable notes: no
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K387003_sequence/1

BBa_K1401008
type: ComponentDefinition
persistent identity: BBa K1401008
display id: BBa_K1401008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tandem Promoter pBad-pTet
creator: Kathleen Lewis
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1779_
m user id: 0
owner id: 22443
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. The promoter is induced by atc and arabinose. The characterization data will be updated before the jamboree.
mutable notes: We looked through the literature and decided to attach the two promoters without any extra sequence between them
mutable provenance: This was formed using BBa_I13453 and BBa_R0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1401008_sequence/1

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0015), reversed
creator: Caitlin Conboy
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010
sequence annotation: B0012
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
direction: direction/reverse
top level: BBa_B0025
start: 50
end: 129
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
direction: direction/reverse
top level: BBa_B0025
start: 1
end: 41
orientation: inline

BBa_I13507
type: ComponentDefinition
persistent identity: BBa I13507
display id: BBa_I13507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E1010
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13507_sequence/1

BBa_B0034
type: Component
persistent identity: component1524815
display id: component1524815
version: 1
top level: BBa_I13507
access: public
definition: BBa_B0034

BBa_E1010
type: Component
persistent identity: component1524822
display id: component1524822
version: 1
top level: BBa_I13507
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1524838
display id: component1524838
version: 1
top level: BBa_I13507
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1524828
display id: component1524828
version: 1
top level: BBa_I13507
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524838
display id: annotation1524838
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
type: Range
persistent identity: range1524838
display id: range1524838
version: 1
direction: direction/forward
top level: BBa_I13507
start: 821
end: 861
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524815
display id: annotation1524815
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
type: Range
persistent identity: range1524815
display id: range1524815
version: 1
direction: direction/forward
top level: BBa_I13507
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524822
display id: annotation1524822
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
type: Range
persistent identity: range1524822
display id: range1524822
version: 1
direction: direction/forward
top level: BBa_I13507
start: 19
end: 699
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524828
display id: annotation1524828
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
type: Range
persistent identity: range1524828
display id: range1524828
version: 1
direction: direction/forward
top level: BBa_I13507
start: 733
end: 812
orientation: inline

BBa_K1152008
type: ComponentDefinition
persistent identity: BBa K1152008
display id: BBa_K1152008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IndC Indigoidine Synthetase (cds)
creator: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1464_
m user id: 0
owner id: 16405
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The IndC Indigoidine Synthetase from Photorhabdus luminescens laumondii TT01 is a nonribosomal peptide synthetase (NRPS) which produces the blue pigment indigoidine. Indigoidine itself consists of two cyclic glutamines. IndC is a single module NRPS and consists of an Adenylation-, Thiolation- and a Thioesterase-domain. The A-domain thus contains an internal Oxidation domain.
mutable notes: The native indC contains an internal EcoRI and SpeI cutting site which was mutated using a CPEC approach.
mutable provenance: The coding sequence was amplified from Photorhabdus luminescens laumondii TT01 DSM15139.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: indC indigoidine synthetase (cds)
sequence annotation: silent mutation a-to-g
sequence annotation: silent mutation t-to-g
sequence: https://synbiohub.org/public/igem/BBa_K1152008_sequence/1

indC indigoidine synthetase (cds)
type: SequenceAnnotation
persistent identity: annotation2369272
display id: annotation2369272
version: 1
top level: BBa_K1152008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1152008/annotation2369272/range2369272/1

https://synbiohub.org/public/igem/BBa_K1152008/annotation2369272/range2369272/1
type: Range
persistent identity: range2369272
display id: range2369272
version: 1
direction: direction/forward
top level: BBa_K1152008
start: 1
end: 3855
orientation: inline

silent mutation a-to-g
type: SequenceAnnotation
persistent identity: annotation2369455
display id: annotation2369455
version: 1
top level: BBa_K1152008
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1152008/annotation2369455/range2369455/1

https://synbiohub.org/public/igem/BBa_K1152008/annotation2369455/range2369455/1
type: Range
persistent identity: range2369455
display id: range2369455
version: 1
direction: direction/forward
top level: BBa_K1152008
start: 212
end: 212
orientation: inline

silent mutation t-to-g
type: SequenceAnnotation
persistent identity: annotation2369456
display id: annotation2369456
version: 1
top level: BBa_K1152008
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1152008/annotation2369456/range2369456/1

https://synbiohub.org/public/igem/BBa_K1152008/annotation2369456/range2369456/1
type: Range
persistent identity: range2369456
display id: range2369456
version: 1
direction: direction/forward
top level: BBa_K1152008
start: 513
end: 513
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline repressor from transposon Tn10 (+LVA)
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SsrA
sequence annotation: tetR
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
direction: direction/forward
top level: tetR
start: 621
end: 654
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
direction: direction/forward
top level: tetR
start: 4
end: 620
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
direction: direction/forward
top level: tetR
start: 661
end: 685
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (Elowitz 1999) -- defines RBS efficiency
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS based on Elowitz repressilator.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
direction: direction/forward
top level: BBa_B0034
start: 5
end: 8
orientation: inline

BBa_K1467101
type: ComponentDefinition
persistent identity: BBa K1467101
display id: BBa_K1467101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible 35s Promoter
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:36:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: 35S is a plant specific promoter obtained from Cauliflower Mosaic Virus. The part is intended for use as a constitutive promoter for gene expression experiments. The part is compatible with GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences).
mutable notes: Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences).
mutable provenance: Cauliflower Mosaic Virus (CaMV)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467101_sequence/1

BBa_K1114012
type: ComponentDefinition
persistent identity: BBa K1114012
display id: BBa_K1114012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23109 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: __NOTOC__ BBa_K1114012 short BBa_K1114012 SequenceAndFeatures ===Design Notes=== This is the MoClo formatted part of BBa_J23109 with AB fusion sites. See the BBa_J23109 page for full information on the part. This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. ===Source=== iGEM Distribution Kit ===References===
mutable notes: __NOTOC__ BBa_K1114033 short BBa_K1114033 SequenceAndFeatures ===Design Notes=== This is the MoClo formatted part of BBa_J23109 with FB fusion sites. See the BBa_J23109 page for full information on the part. This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites. ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114012_sequence/1

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lacI regulated, lambda pL hybrid)
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lac O1
sequence annotation: -10
sequence annotation: BBa_R0011
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
direction: direction/forward
top level: lacI+pL
start: 26
end: 42
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
direction: direction/forward
top level: lacI+pL
start: 3
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
direction: direction/forward
top level: lacI+pL
start: 43
end: 48
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
direction: direction/forward
top level: lacI+pL
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
direction: direction/forward
top level: lacI+pL
start: 20
end: 25
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI repressor from E. coli (+LVA)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0012
sequence annotation: LVA
sequence annotation: lacI-LVA
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
direction: direction/forward
top level: lacI
start: 1129
end: 1153
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
direction: direction/forward
top level: lacI
start: 1090
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

BBa_K1114020
type: ComponentDefinition
persistent identity: BBa K1114020
display id: BBa_K1114020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23117 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_J23117 with AB fusion sites. See the BBa_J23117 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_J23117 with AB fusion sites. See the BBa_J23117 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114020_sequence/1

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

BBa_K1390003
type: ComponentDefinition
persistent identity: BBa K1390003
display id: BBa_K1390003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mmoD
creator: Stefan D??bel
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mmoD is one subunit of the mmo from Methylococcus capsulatus bath
mutable notes: the brick is constructed in the RCF10 standard
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390003_sequence/1

BBa_K1385000
type: ComponentDefinition
persistent identity: BBa K1385000
display id: BBa_K1385000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CPCG2 promoter -> TetR
creator: Benjamin Huang
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1762_
m user id: 0
owner id: 20607
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: CcaR-TetR
mutable notes: RBS
mutable provenance: Genes from PJT122 (Tabor lab)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
sequence annotation: TetR Stop Codon
sequence annotation: TetR Start Codon
sequence annotation: TetR
sequence annotation: CpcG2 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1385000_sequence/1

TetR Stop Codon
type: SequenceAnnotation
persistent identity: annotation2418611
display id: annotation2418611
version: 1
top level: BBa_K1385000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1385000/annotation2418611/range2418611/1

https://synbiohub.org/public/igem/BBa_K1385000/annotation2418611/range2418611/1
type: Range
persistent identity: range2418611
display id: range2418611
version: 1
direction: direction/forward
top level: BBa_K1385000
start: 948
end: 950
orientation: inline

TetR Start Codon
type: SequenceAnnotation
persistent identity: annotation2418610
display id: annotation2418610
version: 1
top level: BBa_K1385000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1385000/annotation2418610/range2418610/1

https://synbiohub.org/public/igem/BBa_K1385000/annotation2418610/range2418610/1
type: Range
persistent identity: range2418610
display id: range2418610
version: 1
direction: direction/forward
top level: BBa_K1385000
start: 327
end: 329
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation2418609
display id: annotation2418609
version: 1
top level: BBa_K1385000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1385000/annotation2418609/range2418609/1

https://synbiohub.org/public/igem/BBa_K1385000/annotation2418609/range2418609/1
type: Range
persistent identity: range2418609
display id: range2418609
version: 1
direction: direction/forward
top level: BBa_K1385000
start: 327
end: 950
orientation: inline

CpcG2 Promoter
type: SequenceAnnotation
persistent identity: annotation2418608
display id: annotation2418608
version: 1
top level: BBa_K1385000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1385000/annotation2418608/range2418608/1

https://synbiohub.org/public/igem/BBa_K1385000/annotation2418608/range2418608/1
type: Range
persistent identity: range2418608
display id: range2418608
version: 1
direction: direction/forward
top level: BBa_K1385000
start: 1
end: 238
orientation: inline

BBa_E0044
type: ComponentDefinition
persistent identity: BBa E0044
display id: BBa_E0044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
creator: jcbraff
created: 2004-09-29T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP-AAV [Pst1 site in earlier version knocked out]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0044_sequence/1

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c2 repressor from Salmonella phage P22 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0053
sequence annotation: stop
sequence annotation: LVA
sequence annotation: cII p22
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 687
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
direction: direction/forward
top level: c2 P22
start: 682
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
direction: direction/forward
top level: c2 P22
start: 649
end: 681
orientation: inline

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 648
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
direction: direction/forward
top level: c2 P22
start: 688
end: 712
orientation: inline

BBa_K1114005
type: ComponentDefinition
persistent identity: BBa K1114005
display id: BBa_K1114005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_J23104 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of BBa_R0079 with EB fusion sites. See the BBa_R0079 page for full information on the part. Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MoClo Fusion Site A
sequence annotation: MoClo Fusion Site B
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K1114005_sequence/1

MoClo Fusion Site A
type: SequenceAnnotation
persistent identity: annotation2361746
display id: annotation2361746
version: 1
top level: BBa_K1114005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114005/annotation2361746/range2361746/1

https://synbiohub.org/public/igem/BBa_K1114005/annotation2361746/range2361746/1
type: Range
persistent identity: range2361746
display id: range2361746
version: 1
direction: direction/forward
top level: BBa_K1114005
start: 1
end: 4
orientation: inline

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361747
display id: annotation2361747
version: 1
top level: BBa_K1114005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114005/annotation2361747/range2361747/1

https://synbiohub.org/public/igem/BBa_K1114005/annotation2361747/range2361747/1
type: Range
persistent identity: range2361747
display id: range2361747
version: 1
direction: direction/forward
top level: BBa_K1114005
start: 40
end: 43
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2361748
display id: annotation2361748
version: 1
top level: BBa_K1114005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1114005/annotation2361748/range2361748/1

https://synbiohub.org/public/igem/BBa_K1114005/annotation2361748/range2361748/1
type: Range
persistent identity: range2361748
display id: range2361748
version: 1
direction: direction/forward
top level: BBa_K1114005
start: 5
end: 39
orientation: inline

BBa_K1114023
type: ComponentDefinition
persistent identity: BBa K1114023
display id: BBa_K1114023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The MoClo format of BBa_I13453 with AB fusion sites.
creator: Jake Awtry
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 17243
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ===Design Notes=== This is the MoClo formatted part of BBa_I13453 with AB fusion sites. See the BBa_I13453 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable notes: ===Design Notes=== This is the MoClo formatted part of BBa_I13453 with AB fusion sites. See the BBa_I13453 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 . ===Source=== iGEM Distribution Kit ===References===
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1114023_sequence/1

BBa_K1114110
type: ComponentDefinition
persistent identity: BBa K1114110
display id: BBa_K1114110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bicistronic design RBS 13, MoClo Format with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 13 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 13 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: MoClo Fusion Site B
sequence annotation: MoClo Fusion Site C
sequence annotation: BCD13
sequence: https://synbiohub.org/public/igem/BBa_K1114110_sequence/1

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361882
display id: annotation2361882
version: 1
top level: BBa_K1114110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114110/annotation2361882/range2361882/1

https://synbiohub.org/public/igem/BBa_K1114110/annotation2361882/range2361882/1
type: Range
persistent identity: range2361882
display id: range2361882
version: 1
direction: direction/forward
top level: BBa_K1114110
start: 1
end: 4
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361883
display id: annotation2361883
version: 1
top level: BBa_K1114110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1114110/annotation2361883/range2361883/1

https://synbiohub.org/public/igem/BBa_K1114110/annotation2361883/range2361883/1
type: Range
persistent identity: range2361883
display id: range2361883
version: 1
direction: direction/forward
top level: BBa_K1114110
start: 89
end: 92
orientation: inline

BCD13
type: SequenceAnnotation
persistent identity: annotation2361884
display id: annotation2361884
version: 1
top level: BBa_K1114110
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1114110/annotation2361884/range2361884/1

https://synbiohub.org/public/igem/BBa_K1114110/annotation2361884/range2361884/1
type: Range
persistent identity: range2361884
display id: range2361884
version: 1
direction: direction/forward
top level: BBa_K1114110
start: 5
end: 88
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: later
mutable notes: N/A
mutable provenance: later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_P0152
type: ComponentDefinition
persistent identity: BBa P0152
display id: BBa_P0152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (434) [S0166]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from 434. Used as the input section for Quad Part Inverter Q01520.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0031
component: BBa_C0052
component: BBa_B0012
sequence annotation: BBa_B0031
sequence annotation: BBa_C0052
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0152_sequence/1

BBa_B0010
type: Component
persistent identity: component944552
display id: component944552
version: 1
top level: BBa_P0152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0031
type: Component
persistent identity: component944535
display id: component944535
version: 1
top level: BBa_P0152
access: public
definition: BBa_B0031

BBa_C0052
type: Component
persistent identity: component944545
display id: component944545
version: 1
top level: BBa_P0152
access: public
definition: cI 434

BBa_B0012
type: Component
persistent identity: component944562
display id: component944562
version: 1
top level: BBa_P0152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944535
display id: annotation944535
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
type: Range
persistent identity: range944535
display id: range944535
version: 1
direction: direction/forward
top level: BBa_P0152
start: 1
end: 14
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation944545
display id: annotation944545
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
type: Range
persistent identity: range944545
display id: range944545
version: 1
direction: direction/forward
top level: BBa_P0152
start: 21
end: 689
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944552
display id: annotation944552
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
type: Range
persistent identity: range944552
display id: range944552
version: 1
direction: direction/forward
top level: BBa_P0152
start: 723
end: 802
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944562
display id: annotation944562
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
type: Range
persistent identity: range944562
display id: range944562
version: 1
direction: direction/forward
top level: BBa_P0152
start: 811
end: 851
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C3 OmpR
sequence annotation: -10
sequence annotation: C2 OmpR
sequence annotation: C1 OmpR
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
direction: direction/forward
top level: OmpR
start: 54
end: 71
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
direction: direction/forward
top level: OmpR
start: 98
end: 103
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
direction: direction/forward
top level: OmpR
start: 34
end: 51
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
direction: direction/forward
top level: OmpR
start: 75
end: 80
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0032.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_E0040
component: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
access: public
definition: GFP

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
access: public
definition: BBa_B0032

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
direction: direction/forward
top level: GFP report
start: 836
end: 876
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
direction: direction/forward
top level: GFP report
start: 748
end: 827
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
direction: direction/forward
top level: GFP report
start: 20
end: 739
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
direction: direction/forward
top level: GFP report
start: 1
end: 13
orientation: inline

BBa_K1680009
type: ComponentDefinition
persistent identity: BBa K1680009
display id: BBa_K1680009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NanoLuc Luciferase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:37:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the NanoLuc(tm) Luziferase (designed by the Promega Corporation, free for research use), Codon optimized for yeast. The Nanoluc is smaller than other luziferase proteins and yields equal or stronger luminescence readings.
mutable notes: Part is shipped in RFC10.
mutable provenance: gBlock designed by Team Tuebingen, sequence by Promega
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NanoLuc
sequence: https://synbiohub.org/public/igem/BBa_K1680009_sequence/1

NanoLuc
type: SequenceAnnotation
persistent identity: annotation2477413
display id: annotation2477413
version: 1
top level: BBa_K1680009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680009/annotation2477413/range2477413/1

https://synbiohub.org/public/igem/BBa_K1680009/annotation2477413/range2477413/1
type: Range
persistent identity: range2477413
display id: range2477413
version: 1
direction: direction/forward
top level: BBa_K1680009
start: 1
end: 516
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage HK022 (+LVA?)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from STHK022N . Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It looks like this extra …
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SsrA
sequence annotation: cI HK022
sequence annotation: OR3 partial
sequence annotation: Help:Barcodes
sequence annotation: 2
sequence annotation: BBa_C0050
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
direction: direction/forward
top level: cI HK022
start: 706
end: 738
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 8
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
direction: direction/forward
top level: cI HK022
start: 745
end: 769
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
direction: direction/forward
top level: cI HK022
start: 739
end: 744
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

BBa_K1114102
type: ComponentDefinition
persistent identity: BBa K1114102
display id: BBa_K1114102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoClo format of a modified version of BBa_B0032 with BC fusion sites
creator: Traci Haddock
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the MoClo formatted part of a modified BBa_B0032 with BC fusion sites. See the BBa_B0032 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable notes: This is the MoClo formatted part of a modified BBa_B0032 with BC fusion sites. See the BBa_B0032 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See <a href: http://parts.igem.org/Part:BBa_K783058???>BBA_K783058 Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
mutable provenance: iGEM Distribution Kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1114102_sequence/1