iGEM interlab 2016 weak device
type: ComponentDefinition
persistent identity: Weak 2016Interlab
display id: Weak_2016Interlab
version: 1
was generated by: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab_SBOLDesignerActivity/1
was generated by: https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab_SBOLDesignerActivity/1
was generated by: https://synbiohub.org/public/iGEM_2016_interlab/Strong_2016Interlab_SBOLDesignerActivity/1
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
type: SO:0000988
type: DnaRegion
role: SO:0000804
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/CDS_Component/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Pro_Component/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Cir_Component/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Ter_Component/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/RBS_Component/1
sequence annotation: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation/1
sequence annotation: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation3/1
sequence annotation: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation4/1
sequence constraint: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceConstraint2/1
sequence constraint: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceConstraint3/1
sequence constraint: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceConstraint/1
sequence constraint: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceConstraint1/1
sequence: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016InterlabSequence/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/CDS_Component/1
type: Component
persistent identity: CDS Component
display id: CDS_Component
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
definition: GFP
access: public

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Pro_Component/1
type: Component
persistent identity: Pro Component
display id: Pro_Component
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
definition: BBa_J23117
access: public

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Cir_Component/1
type: Component
persistent identity: Cir Component
display id: Cir_Component
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
definition: pSB1C3
access: public

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Ter_Component/1
type: Component
persistent identity: Ter Component
display id: Ter_Component
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
definition: BBa_B0015
access: public

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/RBS_Component/1
type: Component
persistent identity: RBS Component
display id: RBS_Component
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
definition: BBa_B0034
access: public

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation/1
type: SequenceAnnotation
persistent identity: Weak 2016Interlab SequenceAnnotation
display id: Weak_2016Interlab_SequenceAnnotation
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
location: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation/Weak_2016Interlab_SequenceAnnotation_Range/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Cir_Component/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation/Weak_2016Interlab_SequenceAnnotation_Range/1
type: Range
persistent identity: Weak 2016Interlab SequenceAnnotation Range
display id: Weak_2016Interlab_SequenceAnnotation_Range
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
start: 1
end: 2070
orientation: inline

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: Weak 2016Interlab SequenceAnnotation1
display id: Weak_2016Interlab_SequenceAnnotation1
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
location: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation1/Weak_2016Interlab_SequenceAnnotation1_Range/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Pro_Component/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation1/Weak_2016Interlab_SequenceAnnotation1_Range/1
type: Range
persistent identity: Weak 2016Interlab SequenceAnnotation1 Range
display id: Weak_2016Interlab_SequenceAnnotation1_Range
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
start: 2071
end: 2105
orientation: inline

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: Weak 2016Interlab SequenceAnnotation2
display id: Weak_2016Interlab_SequenceAnnotation2
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
location: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation2/Weak_2016Interlab_SequenceAnnotation2_Range/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/RBS_Component/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation2/Weak_2016Interlab_SequenceAnnotation2_Range/1
type: Range
persistent identity: Weak 2016Interlab SequenceAnnotation2 Range
display id: Weak_2016Interlab_SequenceAnnotation2_Range
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
start: 2106
end: 2117
orientation: inline

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation3/1
type: SequenceAnnotation
persistent identity: Weak 2016Interlab SequenceAnnotation3
display id: Weak_2016Interlab_SequenceAnnotation3
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
location: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation3/Weak_2016Interlab_SequenceAnnotation3_Range/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/CDS_Component/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation3/Weak_2016Interlab_SequenceAnnotation3_Range/1
type: Range
persistent identity: Weak 2016Interlab SequenceAnnotation3 Range
display id: Weak_2016Interlab_SequenceAnnotation3_Range
version: 1
top level: iGEM interlab 2016 weak device
owned by: myers
owned by: jakebeal
OBI_0001617: 29928012
start: 2118
end: 2837
orientation: inline

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation4/1
type: SequenceAnnotation
persistent identity: Weak 2016Interlab SequenceAnnotation4
display id: Weak_2016Interlab_SequenceAnnotation4
version: 1
top level: iGEM interlab 2016 weak device
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
location: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation4/Weak_2016Interlab_SequenceAnnotation4_Range/1
component: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Ter_Component/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceAnnotation4/Weak_2016Interlab_SequenceAnnotation4_Range/1
type: Range
persistent identity: Weak 2016Interlab SequenceAnnotation4 Range
display id: Weak_2016Interlab_SequenceAnnotation4_Range
version: 1
top level: iGEM interlab 2016 weak device
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
start: 2838
end: 2966
orientation: inline

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceConstraint2/1
type: SequenceConstraint
persistent identity: Weak 2016Interlab SequenceConstraint2
display id: Weak_2016Interlab_SequenceConstraint2
version: 1
top level: iGEM interlab 2016 weak device
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
restriction: precedes
subject: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/RBS_Component/1
object: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/CDS_Component/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceConstraint3/1
type: SequenceConstraint
persistent identity: Weak 2016Interlab SequenceConstraint3
display id: Weak_2016Interlab_SequenceConstraint3
version: 1
top level: iGEM interlab 2016 weak device
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
restriction: precedes
subject: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/CDS_Component/1
object: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Ter_Component/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceConstraint/1
type: SequenceConstraint
persistent identity: Weak 2016Interlab SequenceConstraint
display id: Weak_2016Interlab_SequenceConstraint
version: 1
top level: iGEM interlab 2016 weak device
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
restriction: precedes
subject: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Cir_Component/1
object: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Pro_Component/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Weak_2016Interlab_SequenceConstraint1/1
type: SequenceConstraint
persistent identity: Weak 2016Interlab SequenceConstraint1
display id: Weak_2016Interlab_SequenceConstraint1
version: 1
top level: iGEM interlab 2016 weak device
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
restriction: precedes
subject: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/Pro_Component/1
object: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab/RBS_Component/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

pSB1C3
type: ComponentDefinition
persistent identity: pSB1C3
display id: pSB1C3
version: 1
was generated by: iGEM to SBOL conversion
description: High copy BioBrick assembly plasmid
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: This part was derived from pSB1AC3-P1010.
owned by: james
owned by: myers
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Eliminated ampicillin resistance.
star: true
creator: Austin Che
type: DnaRegion
role: partType/Plasmid Backbone
role: SO:0000755
sequence annotation: BioBrick suffix
sequence annotation: E. coli his operon terminator
sequence annotation: Stem loop
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: rep (pMB1)
sequence annotation: ORI
sequence annotation: T0 terminator
sequence annotation: Chloramphenicol resistance
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: end of terminator
sequence annotation: BioBrick prefix
sequence: https://synbiohub.org/public/igem/pSB1C3_sequence/1

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2119038
display id: annotation2119038
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119038/range2119038/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/pSB1C3/annotation2119038/range2119038/1
type: Range
persistent identity: range2119038
display id: range2119038
version: 1
top level: pSB1C3
direction: direction/forward
start: 1
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2119039
display id: annotation2119039
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119039/range2119039/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/pSB1C3/annotation2119039/range2119039/1
type: Range
persistent identity: range2119039
display id: range2119039
version: 1
top level: pSB1C3
direction: direction/forward
start: 22
end: 93
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2119040
display id: annotation2119040
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119040/range2119040/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/pSB1C3/annotation2119040/range2119040/1
type: Range
persistent identity: range2119040
display id: range2119040
version: 1
top level: pSB1C3
direction: direction/forward
start: 30
end: 64
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2119041
display id: annotation2119041
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119041/range2119041/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/pSB1C3/annotation2119041/range2119041/1
type: Range
persistent identity: range2119041
display id: range2119041
version: 1
top level: pSB1C3
direction: direction/reverse
start: 157
end: 176
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2119042
display id: annotation2119042
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119042/range2119042/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/pSB1C3/annotation2119042/range2119042/1
type: Range
persistent identity: range2119042
display id: range2119042
version: 1
top level: pSB1C3
direction: direction/reverse
start: 261
end: 875
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2119043
display id: annotation2119043
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119043/range2119043/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/pSB1C3/annotation2119043/range2119043/1
type: Range
persistent identity: range2119043
display id: range2119043
version: 1
top level: pSB1C3
direction: direction/forward
start: 276
end: 276
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2119044
display id: annotation2119044
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119044/range2119044/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/pSB1C3/annotation2119044/range2119044/1
type: Range
persistent identity: range2119044
display id: range2119044
version: 1
top level: pSB1C3
direction: direction/forward
start: 1042
end: 1147
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2119045
display id: annotation2119045
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119045/range2119045/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/pSB1C3/annotation2119045/range2119045/1
type: Range
persistent identity: range2119045
display id: range2119045
version: 1
top level: pSB1C3
direction: direction/reverse
start: 1160
end: 1819
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation2119046
display id: annotation2119046
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119046/range2119046/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/pSB1C3/annotation2119046/range2119046/1
type: Range
persistent identity: range2119046
display id: range2119046
version: 1
top level: pSB1C3
direction: direction/forward
start: 1933
end: 1952
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2119047
display id: annotation2119047
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119047/range2119047/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/pSB1C3/annotation2119047/range2119047/1
type: Range
persistent identity: range2119047
display id: range2119047
version: 1
top level: pSB1C3
direction: direction/reverse
start: 2009
end: 2012
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2119048
display id: annotation2119048
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119048/range2119048/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/pSB1C3/annotation2119048/range2119048/1
type: Range
persistent identity: range2119048
display id: range2119048
version: 1
top level: pSB1C3
direction: direction/reverse
start: 2022
end: 2041
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2119049
display id: annotation2119049
version: 1
top level: pSB1C3
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119049/range2119049/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/pSB1C3/annotation2119049/range2119049/1
type: Range
persistent identity: range2119049
display id: range2119049
version: 1
top level: pSB1C3
direction: direction/forward
start: 2051
end: 2072
orientation: inline

BBa_J23117
type: ComponentDefinition
persistent identity: BBa J23117
display id: BBa_J23117
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23117_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016InterlabSequence/1
type: Sequence
persistent identity: Weak 2016InterlabSequence
display id: Weak_2016InterlabSequence
version: 1
was generated by: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab_SBOLDesignerActivity/1
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
elements: tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/pSB1C3_sequence/1
type: Sequence
persistent identity: pSB1C3 sequence
display id: pSB1C3_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23117_sequence/1
type: Sequence
persistent identity: BBa J23117 sequence
display id: BBa_J23117_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggattgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab_SBOLDesignerActivity/1
type: Activity
persistent identity: Medium 2016Interlab SBOLDesignerActivity
display id: Medium_2016Interlab_SBOLDesignerActivity
version: 1
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
creator: Jacob Beal
ended at time: 2018-05-27T11:06:59.843Z
qualified association: https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
top level: https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab_SBOLDesignerActivity/1
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
agent: SBOLDesigner CAD Tool
had role: sequenceEditor

https://synbiohub.org/public/iGEM_2016_interlab/Strong_2016Interlab_SBOLDesignerActivity/1
type: Activity
persistent identity: Strong 2016Interlab SBOLDesignerActivity
display id: Strong_2016Interlab_SBOLDesignerActivity
version: 1
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
creator: Jacob Beal
ended at time: 2018-05-27T11:13:09.084Z
qualified association: https://synbiohub.org/public/iGEM_2016_interlab/Strong_2016Interlab_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/iGEM_2016_interlab/Strong_2016Interlab_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
top level: https://synbiohub.org/public/iGEM_2016_interlab/Strong_2016Interlab_SBOLDesignerActivity/1
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
agent: SBOLDesigner CAD Tool
had role: sequenceEditor

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab_SBOLDesignerActivity/1
type: Activity
persistent identity: Weak 2016Interlab SBOLDesignerActivity
display id: Weak_2016Interlab_SBOLDesignerActivity
version: 1
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
creator: Jacob Beal
ended at time: 2018-05-27T11:16:57.791Z
qualified association: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
top level: https://synbiohub.org/public/iGEM_2016_interlab/Weak_2016Interlab_SBOLDesignerActivity/1
owned by: jakebeal
owned by: myers
OBI_0001617: 29928012
agent: SBOLDesigner CAD Tool
had role: sequenceEditor

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z

SBOLDesigner CAD Tool
type: Agent
persistent identity: SBOLDesigner
display id: SBOLDesigner
version: 3.0
description: SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
modified: 2018-03-02T02:47:25
owned by: myers
OBI_0001617: 28441476
creator: John Gennari
creator: Evren Sirin
creator: Bryan Bartley
creator: Chris Myers
creator: Michael Zhang
creator: Sean Sleight
creator: Michal Galdzicki