BBa_B0102 1 SapI SapI restriction enzyme site (offset cutter) 2007-03-05T12:00:00Z 2015-08-31T04:07:21Z NEB Catalog, SapI is part #R0569S. SapI is an offsite cutter restriction site. The recognition sequence for this enzyme is the 8 bp sequence GCTCTTCN (on the 5' strand). Cleavage at this site results in a 3 bp, 3' overhang (right side, bottom strand) directly downstream of this sequence. This specific part has a T in the 8th position. false false _11_ 0 571 10 Not in stock false This part was designed for use with Heather Keller's alternative assembly method for blunt cloning RBS sequences upstream of a coding sequence. false Heather Keller annotation1920042 1 SapI recognition range1920042 1 1 7 BBa_B0102_sequence 1 gctcttct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z