BBa_B2003
1
T7 0.5
T7 RBS 0.5
2006-09-27T11:00:00Z
2015-08-31T04:07:21Z
Genome of wild-type bacteriophage T7, positions 1476-1495. Includes the -20 to -1 region of gp0.5.
This is the -20 to -1 region of T7 gp0.5, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part B2103.
false
false
_11_
0
571
10
Not in stock
false
The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons:
1) The Shine-Delgarno region is contained within this defined region for all T7 genes.
2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13 of the gene.
false
Heather Keller
annotation1902509
1
SD region
range1902509
1
13
18
BBa_B2003_sequence
1
actttacttatgagggagta
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z